FvH4_5g05230

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006012
Physical Location & Seq
Forward (+)
3048675 .. 3051044
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05230.t2

Sequence Viewer

Length: 894 bp
ATGGAAGCCCAGAAATCCAAGCCAAGCATGAAGAAATCCTCCTCCATAAACTCCGACGACTCCGCCTACACCGCCGAATCCCCCCTCCGCTACCTCTCCCCCATGCGATCCGACGCCTTCGACAGCCCCCCCTACGCCTCCCCGGAGCTCTCCCTCCAGAAGACACCTCCCGACAACTCCAAGGCCATCATTTCCGTCGACAAGCACCCTCCCTTCCGCCCGCAGAAACAGCCGGAGAAAGCTCCGGAGAAATCGCCGTCGCCGATGGTGGTGTACAACAGCCACCGCGCGGCCAGGGAGGACCCGCCGCCTTCCGTGCCGAAGGTGGCGGCGGCTCCCAACGGCGTGGCGAGACAGGCGGGGGACGGCGTTGACGGCGCCGGGGTTGGGAGATCGAGGGCGGCGAGGCGGCCGCCGAGGAGGAGGGAGGATGTGGTGAAGACGGCGGCGCTAGGGTTTCGTGTTAGCGAGGTGGTGCTGTGTTTGGTTTCGTTTTCGGTTATGGCTGCTGATAAAACTCAGGGATGGAGCGGCGACTCGTTTGATCGCTACAAGGAATACAGGTATTGTTTAGCTGTGGCTGTTATTGGATTTGCGTATGCTGCCTTTCAAGCATACAGTCTATCGTATCTACTAGTCACCGGGAAGTATGTGATCAGCCACCACCTCCGTCGCCACTTTGATTTCTTCATGGATCAGGTACTAGCATATCTTCTCATTTCAGCATCATCATCGGCAGCCACCAGGGTCGATGACTGGCAATCGAATTGGGGGAAAGATGAGTTCACGGAGATGGCCACTGCTTCAGTTAGCATGGCCTTCCTTGCTTTCATTGCCTTTGCACTAAGCTCCCTGATCTCTGGTTACGACCTCTTTACCCAAGATTCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

298

Amino Acids

32.59

Weight (kDa)

8.9

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 145 - 235 1.4e-17 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 377
AccBSI CCGCTC 1 cut(s) 531
AccI GTMKAC 1 cut(s) 198
AccII CGCG 2 cut(s) 288, 290
AccIII TCCGGA 1 cut(s) 244
AclWI GGATC 2 cut(s) 102, 702
AcoI YGGCCR 3 cut(s) 291, 410, 795
AcuI CTGAAG 1 cut(s) 789
AcyI GRCGYC 2 cut(s) 114, 378
AfaI GTAC 2 cut(s) 275, 702
AfiI CCNNNNNNNGG 2 cut(s) 289, 387
AgsI TTSAA 1 cut(s) 611
AhlI ACTAGT 1 cut(s) 634
AjnI CCWGG 2 cut(s) 293, 743
AluBI AGCT 4 cut(s) 148, 242, 575, 849
AluI AGCT 4 cut(s) 148, 242, 575, 849
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 2 cut(s) 102, 702
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 5 cut(s) 183, 291, 410, 795, 816
ApeKI GCWGC 3 cut(s) 506, 602, 737
AspLEI GCGC 3 cut(s) 290, 380, 451
AspS9I GGNCC 1 cut(s) 301
AsuC2I CCSGG 3 cut(s) 143, 382, 643
AsuHPI GGTGA 2 cut(s) 448, 631
AvaII GGWCC 1 cut(s) 301
BalI TGGCCA 1 cut(s) 797
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 2 cut(s) 167, 446
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 493, 589, 749
BccI CCATC 4 cut(s) 194, 259, 519, 787
BceAI ACGGC 5 cut(s) 241, 358, 382, 391, 459
BcgI CGANNNNNNTGC 2 cut(s) 714, 748
BciT130I CCWGG 2 cut(s) 295, 745
BclI TGATCA 1 cut(s) 654
BcnI CCSGG 3 cut(s) 143, 382, 643
BcoDI GTCTC 1 cut(s) 346
BcuI ACTAGT 1 cut(s) 634
BfaI CTAG 3 cut(s) 452, 635, 704
BfoI RGCGCY 2 cut(s) 381, 452
Bme1390I CCNGG 5 cut(s) 143, 295, 382, 643, 745
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 3 cut(s) 303, 336, 379
BmrFI CCNGG 5 cut(s) 143, 295, 382, 643, 745
BmsI GCATC 1 cut(s) 734
BpiI GAAGAC 2 cut(s) 167, 446
BpmI CTGGAG 1 cut(s) 140
BpuMI CCSGG 3 cut(s) 143, 382, 643
BsaHI GRCGYC 2 cut(s) 114, 378
BsaJI CCNNGG 6 cut(s) 141, 180, 294, 381, 416, 744
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 387
Bse1I ACTGG 1 cut(s) 761
Bse3DI GCAATG 1 cut(s) 831
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 2 cut(s) 295, 745
BseDI CCNNGG 6 cut(s) 141, 180, 294, 381, 416, 744
BseGI GGATG 2 cut(s) 436, 530
BseLI CCNNNNNNNGG 2 cut(s) 289, 387
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 1 cut(s) 761
BseRI GAGGAG 3 cut(s) 31, 433, 436
BseX3I CGGCCG 1 cut(s) 410
BseXI GCAGC 3 cut(s) 493, 589, 749
Bsh1236I CGCG 2 cut(s) 288, 290
Bsh1285I CGRYCG 1 cut(s) 413
BshFI GGCC 5 cut(s) 185, 293, 412, 797, 818
BshNI GGYRCC 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 5 cut(s) 143, 233, 245, 381, 642
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 289, 387
BsmAI GTCTC 1 cut(s) 346
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 5 cut(s) 185, 293, 412, 797, 818
Bsp1286I GDGCHC 1 cut(s) 150
Bsp13I TCCGGA 1 cut(s) 244
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 6 cut(s) 107, 392, 544, 654, 694, 855
BspANI GGCC 5 cut(s) 185, 293, 412, 797, 818
BspCNI CTCAG 1 cut(s) 532
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 2 cut(s) 288, 290
BspLI GGNNCC 3 cut(s) 303, 336, 379
BspPI GGATC 2 cut(s) 102, 702
BspT107I GGYRCC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 531
BsrDI GCAATG 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 1 cut(s) 761
BssECI CCNNGG 6 cut(s) 141, 180, 294, 381, 416, 744
BssMI GATC 6 cut(s) 107, 392, 544, 654, 694, 855
BssNI GRCGYC 2 cut(s) 114, 378
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 2 cut(s) 295, 745
Bst4CI ACNGT 1 cut(s) 620
BstACI GRCGYC 2 cut(s) 114, 378
BstAUI TGTACA 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 519, 845
BstF5I GGATG 2 cut(s) 436, 530
BstFNI CGCG 2 cut(s) 288, 290
BstH2I RGCGCY 2 cut(s) 381, 452
BstHHI GCGC 3 cut(s) 290, 380, 451
BstKTI GATC 6 cut(s) 110, 395, 547, 657, 697, 858
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 6 cut(s) 107, 392, 544, 654, 694, 855
BstMCI CGRYCG 1 cut(s) 413
BstMWI GCNNNNNNNGC 9 cut(s) 71, 229, 316, 356, 375, 602, 611, 824, 833
BstNI CCWGG 2 cut(s) 295, 745
BstSCI CCNGG 5 cut(s) 141, 293, 380, 641, 743
BstUI CGCG 2 cut(s) 288, 290
BstV1I GCAGC 3 cut(s) 493, 589, 749
BstV2I GAAGAC 2 cut(s) 167, 446
BstXI CCANNNNNNTGG 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 410
BsuRI GGCC 5 cut(s) 185, 293, 412, 797, 818
BtsCI GGATG 2 cut(s) 436, 530
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 1 cut(s) 798
Cac8I GCNNGC 1 cut(s) 221
CciNI GCGGCCGC 1 cut(s) 410
CfoI GCGC 3 cut(s) 290, 380, 451
Cfr13I GGNCC 1 cut(s) 301
CseI GACGC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 274, 701
CviAII CATG 5 cut(s) 28, 103, 691, 814, 891
CviQI GTAC 2 cut(s) 274, 701
DdeI CTNAG 2 cut(s) 519, 845
DinI GGCGCC 1 cut(s) 379
DpnI GATC 6 cut(s) 109, 394, 546, 656, 696, 857
DpnII GATC 6 cut(s) 107, 392, 544, 654, 694, 855
EaeI YGGCCR 3 cut(s) 291, 410, 795
EagI CGGCCG 1 cut(s) 410
EciI GGCGGA 2 cut(s) 52, 206
Ecl136II GAGCTC 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 301
Eco52I CGGCCG 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 789
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 293, 743
EcoT14I CCWWGG 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 5 cut(s) 31, 106, 694, 817, 894
FaqI GGGAC 1 cut(s) 377
FatI CATG 5 cut(s) 27, 102, 690, 813, 890
FauI CCCGC 3 cut(s) 228, 312, 352
FbaI TGATCA 1 cut(s) 654
FblI GTMKAC 1 cut(s) 198
FokI GGATG 2 cut(s) 443, 537
FriOI GRGCYC 1 cut(s) 150
FspBI CTAG 3 cut(s) 452, 635, 704
GlaI GCGC 3 cut(s) 289, 379, 450
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 2 cut(s) 381, 452
HaeIII GGCC 5 cut(s) 185, 293, 412, 797, 818
HapII CCGG 5 cut(s) 143, 233, 245, 381, 642
HgaI GACGC 1 cut(s) 122
HhaI GCGC 3 cut(s) 290, 380, 451
Hin1I GRCGYC 2 cut(s) 114, 378
Hin1II CATG 5 cut(s) 31, 106, 694, 817, 894
Hin6I GCGC 3 cut(s) 288, 378, 449
HinP1I GCGC 3 cut(s) 288, 378, 449
HincII GTYRAC 2 cut(s) 199, 373
HindII GTYRAC 2 cut(s) 199, 373
HinfI GANTC 4 cut(s) 59, 77, 536, 884
HpaII CCGG 5 cut(s) 143, 233, 245, 381, 642
HphI GGTGA 2 cut(s) 448, 631
Hpy166II GTNNAC 4 cut(s) 199, 274, 373, 786
Hpy188I TCNGA 2 cut(s) 55, 112
Hpy188III TCNNGA 3 cut(s) 157, 170, 245
Hpy8I GTNNAC 4 cut(s) 199, 274, 373, 786
Hpy99I CGWCG 5 cut(s) 59, 116, 200, 262, 675
HpyAV CCTTC 5 cut(s) 127, 223, 316, 321, 829
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4V TGCA 1 cut(s) 842
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 229, 316, 356, 375, 602, 611, 824, 833
HpyF3I CTNAG 2 cut(s) 519, 845
Hsp92I GRCGYC 2 cut(s) 114, 378
Hsp92II CATG 5 cut(s) 31, 106, 694, 817, 894
HspAI GCGC 3 cut(s) 288, 378, 449
KasI GGCGCC 1 cut(s) 377
Kpn2I TCCGGA 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 654
Kzo9I GATC 6 cut(s) 107, 392, 544, 654, 694, 855
LmnI GCTCC 5 cut(s) 145, 247, 340, 528, 854
Lsp1109I GCAGC 3 cut(s) 493, 589, 749
LweI GCATC 1 cut(s) 734
MaeI CTAG 3 cut(s) 452, 635, 704
MaeIII GTNAC 2 cut(s) 637, 863
MalI GATC 6 cut(s) 109, 394, 546, 656, 696, 857
MbiI CCGCTC 1 cut(s) 531
MboI GATC 6 cut(s) 107, 392, 544, 654, 694, 855
MboII GAAGA 5 cut(s) 43, 172, 451, 679, 704
MhlI GDGCHC 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 1 cut(s) 766
MluNI TGGCCA 1 cut(s) 797
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 2 cut(s) 53, 530
MmeI TCCRAC 2 cut(s) 78, 135
Mox20I TGGCCA 1 cut(s) 797
MroI TCCGGA 1 cut(s) 244
MscI TGGCCA 1 cut(s) 797
MslI CAYNNNNRTG 1 cut(s) 791
Msp20I TGGCCA 1 cut(s) 797
MspI CCGG 5 cut(s) 143, 233, 245, 381, 642
MspR9I CCNGG 5 cut(s) 143, 295, 382, 643, 745
MvaI CCWGG 2 cut(s) 295, 745
MvnI CGCG 2 cut(s) 288, 290
MwoI GCNNNNNNNGC 9 cut(s) 71, 229, 316, 356, 375, 602, 611, 824, 833
NarI GGCGCC 1 cut(s) 378
NciI CCSGG 3 cut(s) 143, 382, 643
NdeII GATC 6 cut(s) 107, 392, 544, 654, 694, 855
NlaIII CATG 5 cut(s) 31, 106, 694, 817, 894
NlaIV GGNNCC 3 cut(s) 303, 336, 379
NmeAIII GCCGAG 1 cut(s) 441
NmuCI GTSAC 1 cut(s) 637
NotI GCGGCCGC 1 cut(s) 410
PcsI WCGNNNNNNNCGW 2 cut(s) 260, 401
PfeI GAWTC 2 cut(s) 77, 884
PleI GAGTC 2 cut(s) 53, 530
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 2 cut(s) 53, 530
PpuMI RGGWCCY 1 cut(s) 301
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 293, 743
PspGI CCWGG 2 cut(s) 293, 743
PspN4I GGNNCC 3 cut(s) 303, 336, 379
PspPI GGNCC 1 cut(s) 301
PspPPI RGGWCCY 1 cut(s) 301
RsaI GTAC 2 cut(s) 275, 702
RsaNI GTAC 2 cut(s) 274, 701
RseI CAYNNNNRTG 1 cut(s) 791
SacI GAGCTC 1 cut(s) 150
SalI GTCGAC 1 cut(s) 197
Sau3AI GATC 6 cut(s) 107, 392, 544, 654, 694, 855
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 2 cut(s) 53, 530
ScrFI CCNGG 5 cut(s) 143, 295, 382, 643, 745
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 1 cut(s) 734
SfoI GGCGCC 1 cut(s) 379
SinI GGWCC 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 791
SpeI ACTAGT 1 cut(s) 634
Sse9I AATT 1 cut(s) 766
SspDI GGCGCC 1 cut(s) 377
SspMI CTAG 3 cut(s) 452, 635, 704
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 5 cut(s) 141, 293, 380, 641, 743
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 620
TaqI TCGA 5 cut(s) 120, 198, 395, 750, 764
TasI AATT 1 cut(s) 766
TatI WGTACW 1 cut(s) 273
TauI GCSGC 9 cut(s) 293, 310, 332, 335, 404, 412, 415, 449, 534
TfiI GAWTC 2 cut(s) 77, 884
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 3 cut(s) 506, 602, 737
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 44, 679, 820
TspGWI ACGGA 4 cut(s) 184, 304, 659, 803
TspRI CASTG 1 cut(s) 805
VpaK11BI GGWCC 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 3 cut(s) 452, 635, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.