MD06G1175400.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006012
Physical Location & Seq
Reverse (-)
31501633 .. 31503275
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1175400.v1.1.491

Sequence Viewer

Length: 918 bp
ATGAAGAAGTCCTGGTCTTTGAACTCCGACGACTCACCCCTCCGATTCCACTCCCCGATCCGATCCGACGGCTTCGACCCGACCGACAGCCCGCCTTACCAGTCCCCCGAGAACTCGCCGGAGAAGCCGGTCGACAACTCCAAGGCGATCATGCCCGTCGACAAGTTCAACCAGTACCCGCCGCCCCGGCATCAGAAGCGGCCGGAGAACGCCAAGGCGCTGTCGCCGGTGGTGGTGTACAACCGGTCGGTGAAGGAGGAGGTGGCGCCGTCCGTGTCGAAGGTTGGGCCGACCGGCGTAGTCAACGGTGTTGGAGGCGGGGAGGAAGGCGGAGTCAACGGTGTTGGCGGCGGAGGTGGAGGCGGTGGGAGATCCAAGGGGGTGACGAATTCGATTCTGAGACGGTCGAAGAGGGGGGAGATGATGAACACGGCGGCGCTGGGGTTTAGGGTGAGTGAGTTGGTTTTGTGCTTGATTTCGTTCTCGGTTATGGCGGCGGACAAGACTCAGGGATGGAGCGGCGACTCCTTCGACCGCTACAAGGAATACAGGTATTGCTTAGCTGTGAATGTAATTGGATTTGCATATGCTGCCTTTCAAACCTACAATCTATCCTACCATCTAGTCACCGGGAAGTTCGTGATCAGCCACCGTCTTCGTTGCAACTTTGATTTTTTCATGGATCAGGCAAGCTCCTCATCCCGCCCATCTGTCTTCATCGCTGTGCTGGCATACCTTCTCATTTCTGCATCATCATCTGCAGCCACTCGGGTGGATGACTGGCAATCGAATTGGGGGAAAGATGAGTTCACGGAGATGGCCACTGCTTCGGTTAGCATGGCTTTCCTGGCTTTTGTTGCCTTTGCGCTTAGCTCCCTCATATCTGGTTACAACCTCTGTACCCACGACTCTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

306

Amino Acids

33.04

Weight (kDa)

8.57

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 142 - 287 1.1e-24 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 519
AccI GTMKAC 2 cut(s) 132, 159
AclWI GGATC 4 cut(s) 52, 57, 366, 690
AcoI YGGCCR 2 cut(s) 200, 819
AcsI RAATTY 1 cut(s) 388
AcyI GRCGYC 1 cut(s) 266
AfaI GTAC 3 cut(s) 176, 239, 901
AfiI CCNNNNNNNGG 1 cut(s) 541
AgeI ACCGGT 1 cut(s) 243
AgsI TTSAA 3 cut(s) 22, 169, 599
AjnI CCWGG 2 cut(s) 11, 846
AleI CACNNNNGTG 1 cut(s) 770
AluBI AGCT 3 cut(s) 563, 693, 873
AluI AGCT 3 cut(s) 563, 693, 873
Alw26I GTCTC 1 cut(s) 394
AlwI GGATC 4 cut(s) 52, 57, 366, 690
Ama87I CYCGRG 2 cut(s) 107, 768
AoxI GGCC 3 cut(s) 200, 287, 819
ApeKI GCWGC 2 cut(s) 590, 761
ApoI RAATTY 1 cut(s) 388
AsiGI ACCGGT 1 cut(s) 243
AspLEI GCGC 4 cut(s) 220, 268, 439, 868
AspS9I GGNCC 1 cut(s) 287
AsuC2I CCSGG 2 cut(s) 187, 631
AsuHPI GGTGA 5 cut(s) 27, 262, 394, 463, 619
AvaI CYCGRG 2 cut(s) 107, 768
BaeI ACNNNNGTAYC 2 cut(s) 883, 916
BalI TGGCCA 1 cut(s) 821
BanI GGYRCC 1 cut(s) 265
BbsI GAAGAC 2 cut(s) 647, 706
BbvI GCAGC 2 cut(s) 577, 773
BccI CCATC 4 cut(s) 507, 627, 715, 811
BceAI ACGGC 3 cut(s) 85, 253, 447
BciT130I CCWGG 2 cut(s) 13, 848
BclI TGATCA 1 cut(s) 642
BcnI CCSGG 2 cut(s) 187, 631
BcoDI GTCTC 1 cut(s) 394
BfaI CTAG 1 cut(s) 623
BfmI CTRYAG 1 cut(s) 759
BfoI RGCGCY 3 cut(s) 221, 269, 440
BglI GCCNNNNNGGC 1 cut(s) 187
BisI GCNGC 8 cut(s) 182, 200, 349, 435, 495, 520, 591, 762
BlpI GCTNAGC 2 cut(s) 559, 869
BlsI GCNGC 8 cut(s) 183, 201, 350, 436, 496, 521, 592, 763
Bme1390I CCNGG 4 cut(s) 13, 187, 631, 848
BmeT110I CYCGRG 2 cut(s) 107, 768
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 4 cut(s) 13, 187, 631, 848
BmsI GCATC 2 cut(s) 199, 758
BpiI GAAGAC 2 cut(s) 647, 706
Bpu1102I GCTNAGC 2 cut(s) 559, 869
BpuMI CCSGG 2 cut(s) 187, 631
BsaHI GRCGYC 1 cut(s) 266
BsaJI CCNNGG 4 cut(s) 141, 185, 213, 375
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse118I RCCGGY 4 cut(s) 127, 226, 243, 293
Bse1I ACTGG 3 cut(s) 100, 172, 785
BseBI CCWGG 2 cut(s) 13, 848
BseDI CCNNGG 4 cut(s) 141, 185, 213, 375
BseGI GGATG 3 cut(s) 518, 698, 781
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMII CTCAG 2 cut(s) 389, 521
BseNI ACTGG 3 cut(s) 100, 172, 785
BseRI GAGGAG 2 cut(s) 272, 685
BseX3I CGGCCG 1 cut(s) 200
BseXI GCAGC 2 cut(s) 577, 773
BseYI CCCAGC 1 cut(s) 439
Bsh1285I CGRYCG 7 cut(s) 84, 132, 203, 248, 294, 407, 535
BshFI GGCC 3 cut(s) 202, 289, 821
BshNI GGYRCC 1 cut(s) 265
BshTI ACCGGT 1 cut(s) 243
BsiEI CGRYCG 7 cut(s) 84, 132, 203, 248, 294, 407, 535
BsiHKCI CYCGRG 2 cut(s) 107, 768
BsiSI CCGG 8 cut(s) 119, 128, 187, 203, 227, 244, 294, 630
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 1 cut(s) 394
BsmBI CGTCTC 1 cut(s) 394
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 3 cut(s) 202, 289, 821
BsoBI CYCGRG 2 cut(s) 107, 768
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 6 cut(s) 57, 62, 147, 371, 642, 682
Bsp1720I GCTNAGC 2 cut(s) 559, 869
BspANI GGCC 3 cut(s) 202, 289, 821
BspCNI CTCAG 2 cut(s) 390, 520
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 763
BspPI GGATC 4 cut(s) 52, 57, 366, 690
BspT107I GGYRCC 1 cut(s) 265
BsrBI CCGCTC 1 cut(s) 519
BsrFI RCCGGY 4 cut(s) 127, 226, 243, 293
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 3 cut(s) 100, 172, 785
BssAI RCCGGY 4 cut(s) 127, 226, 243, 293
BssECI CCNNGG 4 cut(s) 141, 185, 213, 375
BssMI GATC 6 cut(s) 57, 62, 147, 371, 642, 682
BssNI GRCGYC 1 cut(s) 266
BssT1I CCWWGG 3 cut(s) 141, 213, 375
Bst2UI CCWGG 2 cut(s) 13, 848
Bst4CI ACNGT 4 cut(s) 308, 341, 405, 653
Bst6I CTCTTC 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 92, 691, 729
BstDEI CTNAG 4 cut(s) 398, 507, 559, 869
BstF5I GGATG 3 cut(s) 518, 698, 781
BstH2I RGCGCY 3 cut(s) 221, 269, 440
BstHHI GCGC 4 cut(s) 220, 268, 439, 868
BstKTI GATC 6 cut(s) 60, 65, 150, 374, 645, 685
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 6 cut(s) 57, 62, 147, 371, 642, 682
BstMCI CGRYCG 7 cut(s) 84, 132, 203, 248, 294, 407, 535
BstMWI GCNNNNNNNGC 7 cut(s) 124, 187, 196, 590, 728, 848, 857
BstNI CCWGG 2 cut(s) 13, 848
BstSCI CCNGG 4 cut(s) 11, 185, 629, 846
BstSFI CTRYAG 1 cut(s) 759
BstV1I GCAGC 2 cut(s) 577, 773
BstV2I GAAGAC 2 cut(s) 647, 706
BstX2I RGATCY 1 cut(s) 371
BstXI CCANNNNNNTGG 1 cut(s) 772
BstYI RGATCY 1 cut(s) 371
BstZI CGGCCG 1 cut(s) 200
BsuRI GGCC 3 cut(s) 202, 289, 821
BtgZI GCGATG 1 cut(s) 703
BtsCI GGATG 3 cut(s) 518, 698, 781
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 1 cut(s) 822
Cac8I GCNNGC 3 cut(s) 92, 691, 729
CfoI GCGC 4 cut(s) 220, 268, 439, 868
Cfr10I RCCGGY 4 cut(s) 127, 226, 243, 293
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 175, 238, 900
CspAI ACCGGT 1 cut(s) 243
CviAII CATG 3 cut(s) 151, 679, 838
CviQI GTAC 3 cut(s) 175, 238, 900
DdeI CTNAG 4 cut(s) 398, 507, 559, 869
DinI GGCGCC 1 cut(s) 267
DpnI GATC 6 cut(s) 59, 64, 149, 373, 644, 684
DpnII GATC 6 cut(s) 57, 62, 147, 371, 642, 682
EaeI YGGCCR 2 cut(s) 200, 819
EagI CGGCCG 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
EciI GGCGGA 3 cut(s) 345, 366, 512
EclXI CGGCCG 1 cut(s) 200
Eco130I CCWWGG 3 cut(s) 141, 213, 375
Eco52I CGGCCG 1 cut(s) 200
Eco88I CYCGRG 2 cut(s) 107, 768
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 11, 846
EcoT14I CCWWGG 3 cut(s) 141, 213, 375
EgeI GGCGCC 1 cut(s) 267
EheI GGCGCC 1 cut(s) 267
ErhI CCWWGG 3 cut(s) 141, 213, 375
Esp3I CGTCTC 1 cut(s) 394
FaeI CATG 3 cut(s) 154, 682, 841
FaiI YATR 8 cut(s) 152, 491, 586, 588, 680, 733, 839, 881
FaqI GGGAC 1 cut(s) 88
FatI CATG 3 cut(s) 150, 678, 837
FauI CCCGC 4 cut(s) 99, 186, 311, 710
FauNDI CATATG 1 cut(s) 586
FbaI TGATCA 1 cut(s) 642
FblI GTMKAC 2 cut(s) 132, 159
Fnu4HI GCNGC 8 cut(s) 182, 200, 349, 435, 495, 520, 591, 762
FokI GGATG 3 cut(s) 525, 685, 788
Fsp4HI GCNGC 8 cut(s) 182, 200, 349, 435, 495, 520, 591, 762
FspBI CTAG 1 cut(s) 623
GlaI GCGC 4 cut(s) 219, 267, 438, 867
GluI GCNGC 8 cut(s) 182, 200, 349, 435, 495, 520, 591, 762
GsaI CCCAGC 1 cut(s) 443
HaeII RGCGCY 3 cut(s) 221, 269, 440
HaeIII GGCC 3 cut(s) 202, 289, 821
HapII CCGG 8 cut(s) 119, 128, 187, 203, 227, 244, 294, 630
HhaI GCGC 4 cut(s) 220, 268, 439, 868
Hin1I GRCGYC 1 cut(s) 266
Hin1II CATG 3 cut(s) 154, 682, 841
Hin6I GCGC 4 cut(s) 218, 266, 437, 866
HinP1I GCGC 4 cut(s) 218, 266, 437, 866
HincII GTYRAC 4 cut(s) 133, 160, 304, 337
HindII GTYRAC 4 cut(s) 133, 160, 304, 337
HinfI GANTC 7 cut(s) 32, 45, 333, 394, 505, 524, 908
HpaII CCGG 8 cut(s) 119, 128, 187, 203, 227, 244, 294, 630
HphI GGTGA 5 cut(s) 27, 262, 394, 463, 619
Hpy166II GTNNAC 6 cut(s) 133, 160, 238, 304, 337, 810
Hpy188I TCNGA 6 cut(s) 28, 44, 62, 67, 195, 399
Hpy188III TCNNGA 1 cut(s) 640
Hpy8I GTNNAC 6 cut(s) 133, 160, 238, 304, 337, 810
Hpy99I CGWCG 3 cut(s) 32, 71, 161
HpyAV CCTTC 5 cut(s) 247, 274, 320, 538, 746
HpyCH4III ACNGT 4 cut(s) 308, 341, 405, 653
HpyCH4V TGCA 4 cut(s) 584, 663, 749, 761
HpyF10VI GCNNNNNNNGC 7 cut(s) 124, 187, 196, 590, 728, 848, 857
HpyF3I CTNAG 4 cut(s) 398, 507, 559, 869
Hsp92I GRCGYC 1 cut(s) 266
Hsp92II CATG 3 cut(s) 154, 682, 841
HspAI GCGC 4 cut(s) 218, 266, 437, 866
KasI GGCGCC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 642
Kzo9I GATC 6 cut(s) 57, 62, 147, 371, 642, 682
LmnI GCTCC 3 cut(s) 516, 698, 878
Lsp1109I GCAGC 2 cut(s) 577, 773
LweI GCATC 2 cut(s) 199, 758
MaeI CTAG 1 cut(s) 623
MaeIII GTNAC 3 cut(s) 382, 625, 887
MalI GATC 6 cut(s) 59, 64, 149, 373, 644, 684
MbiI CCGCTC 1 cut(s) 519
MboI GATC 6 cut(s) 57, 62, 147, 371, 642, 682
MboII GAAGA 4 cut(s) 16, 421, 647, 706
MflI RGATCY 1 cut(s) 371
MlsI TGGCCA 1 cut(s) 821
MluCI AATT 3 cut(s) 388, 573, 790
MluNI TGGCCA 1 cut(s) 821
Mly113I GGCGCC 1 cut(s) 266
MlyI GAGTC 5 cut(s) 26, 342, 499, 518, 902
MmeI TCCRAC 3 cut(s) 51, 90, 292
Mox20I TGGCCA 1 cut(s) 821
MscI TGGCCA 1 cut(s) 821
MslI CAYNNNNRTG 3 cut(s) 722, 770, 815
Msp20I TGGCCA 1 cut(s) 821
MspI CCGG 8 cut(s) 119, 128, 187, 203, 227, 244, 294, 630
MspR9I CCNGG 4 cut(s) 13, 187, 631, 848
MvaI CCWGG 2 cut(s) 13, 848
MwoI GCNNNNNNNGC 7 cut(s) 124, 187, 196, 590, 728, 848, 857
NarI GGCGCC 1 cut(s) 266
NciI CCSGG 2 cut(s) 187, 631
NdeI CATATG 1 cut(s) 586
NdeII GATC 6 cut(s) 57, 62, 147, 371, 642, 682
NlaIII CATG 3 cut(s) 154, 682, 841
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 382, 625
OliI CACNNNNGTG 1 cut(s) 770
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 2 cut(s) 45, 394
PinAI ACCGGT 1 cut(s) 243
PkrI GCNGC 8 cut(s) 183, 201, 350, 436, 496, 521, 592, 763
PleI GAGTC 5 cut(s) 26, 341, 499, 518, 902
PluTI GGCGCC 1 cut(s) 269
PpsI GAGTC 5 cut(s) 26, 341, 499, 518, 902
Psp6I CCWGG 2 cut(s) 11, 846
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 2 cut(s) 11, 846
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 287
PstI CTGCAG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 3 cut(s) 176, 239, 901
RsaNI GTAC 3 cut(s) 175, 238, 900
RseI CAYNNNNRTG 3 cut(s) 722, 770, 815
SalI GTCGAC 2 cut(s) 131, 158
SatI GCNGC 8 cut(s) 182, 200, 349, 435, 495, 520, 591, 762
Sau3AI GATC 6 cut(s) 57, 62, 147, 371, 642, 682
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 5 cut(s) 26, 342, 499, 518, 902
ScrFI CCNGG 4 cut(s) 13, 187, 631, 848
SfaNI GCATC 2 cut(s) 199, 758
SfcI CTRYAG 1 cut(s) 759
SfoI GGCGCC 1 cut(s) 267
SgrAI CRCCGGYG 1 cut(s) 226
SmiMI CAYNNNNRTG 3 cut(s) 722, 770, 815
Sse9I AATT 3 cut(s) 388, 573, 790
SspDI GGCGCC 1 cut(s) 265
SspMI CTAG 1 cut(s) 623
StyD4I CCNGG 4 cut(s) 11, 185, 629, 846
StyI CCWWGG 3 cut(s) 141, 213, 375
TaaI ACNGT 4 cut(s) 308, 341, 405, 653
TaqI TCGA 8 cut(s) 75, 132, 159, 278, 392, 407, 531, 788
TaqII GACCGA 1 cut(s) 98
TasI AATT 3 cut(s) 388, 573, 790
TatI WGTACW 1 cut(s) 237
TauI GCSGC 6 cut(s) 184, 202, 351, 437, 497, 522
TfiI GAWTC 2 cut(s) 45, 394
TscAI CASTG 1 cut(s) 829
TseFI GTSAC 2 cut(s) 382, 625
TseI GCWGC 2 cut(s) 590, 761
Tsp45I GTSAC 2 cut(s) 382, 625
TspDTI ATGAA 4 cut(s) 17, 440, 667, 706
TspGWI ACGGA 2 cut(s) 262, 827
TspRI CASTG 1 cut(s) 829
XapI RAATTY 1 cut(s) 388
XmiI GTMKAC 2 cut(s) 132, 159
XspI CTAG 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.