MD13G1274000.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006012
Physical Location & Seq
Forward (+)
37140509 .. 37140790
282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1274000.v1.1.491

Sequence Viewer

Length: 282 bp
ATGGAGAATCTATCCTACCATCTAGTCACCGGGAAGTTCGTGATCAGCCACCGTCTTCGTTGCAACTTTGATTTTTTCATGGATCAGGTGTTGGCATACCTTCTCATTTCTGCATCATCATCTGCAGCCACTTGGGTCGATGAATGGCAATCGAATTGGGGGAAAGATGAGTTCATGGAGATGGTCATTGCTTCGGTTAGCATGGCATTCTTGGCTTTTGTTGCCTTTGCGCTTAACTCTCTCATATCTGGTTACATCCTTTGTACCCACGACTCTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

94

Amino Acids

10.51

Weight (kDa)

4.76

Isoelectric Point (pI)

16.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 6 - 75 1.7e-12 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 90
AfaI GTAC 1 cut(s) 265
AlwI GGATC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 232
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 137
BccI CCATC 2 cut(s) 27, 175
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 1 cut(s) 31
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 186
BseGI GGATG 1 cut(s) 255
BseMI GCAATG 1 cut(s) 186
BseXI GCAGC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 30
BsmI GAATGC 1 cut(s) 206
Bsp143I GATC 2 cut(s) 42, 82
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 186
BssMI GATC 2 cut(s) 42, 82
Bst4CI ACNGT 1 cut(s) 53
BstF5I GGATG 1 cut(s) 255
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 2 cut(s) 45, 85
BstMBI GATC 2 cut(s) 42, 82
BstMWI GCNNNNNNNGC 2 cut(s) 212, 221
BstSCI CCNGG 1 cut(s) 29
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 137
BstV2I GAAGAC 1 cut(s) 47
BtsCI GGATG 1 cut(s) 255
CfoI GCGC 1 cut(s) 232
Csp6I GTAC 1 cut(s) 264
CviAII CATG 3 cut(s) 79, 175, 202
CviJI RGCY 3 cut(s) 48, 128, 215
CviKI_1 RGCY 3 cut(s) 48, 128, 215
CviQI GTAC 1 cut(s) 264
DpnI GATC 2 cut(s) 44, 84
DpnII GATC 2 cut(s) 42, 82
FaeI CATG 3 cut(s) 82, 178, 205
FaiI YATR 5 cut(s) 80, 97, 176, 203, 245
FatI CATG 3 cut(s) 78, 174, 201
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 231
GluI GCNGC 1 cut(s) 126
HapII CCGG 1 cut(s) 30
HhaI GCGC 1 cut(s) 232
Hin1II CATG 3 cut(s) 82, 178, 205
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 2 cut(s) 7, 272
HpaII CCGG 1 cut(s) 30
HphI GGTGA 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4V TGCA 3 cut(s) 63, 113, 125
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 221
Hsp92II CATG 3 cut(s) 82, 178, 205
HspAI GCGC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 2 cut(s) 42, 82
LpnPI CCDG 3 cut(s) 43, 71, 234
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 1 cut(s) 122
MaeI CTAG 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 25, 251
MalI GATC 2 cut(s) 44, 84
MboI GATC 2 cut(s) 42, 82
MboII GAAGA 1 cut(s) 47
MluCI AATT 1 cut(s) 154
MlyI GAGTC 1 cut(s) 266
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 179
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 212, 221
NciI CCSGG 1 cut(s) 31
NdeII GATC 2 cut(s) 42, 82
NlaIII CATG 3 cut(s) 82, 178, 205
NmuCI GTSAC 1 cut(s) 25
PctI GAATGC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PstI CTGCAG 1 cut(s) 127
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 2 cut(s) 42, 82
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 31
SetI ASST 2 cut(s) 90, 102
SfaNI GCATC 1 cut(s) 122
SfcI CTRYAG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 1 cut(s) 154
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 29
TaaI ACNGT 1 cut(s) 53
TaqI TCGA 2 cut(s) 138, 152
TasI AATT 1 cut(s) 154
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 125
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 3 cut(s) 67, 156, 163
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.