Rh7CG141900

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006012
Physical Location & Seq
Reverse (-)
11529656 .. 11530602
947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG141900.1

Sequence Viewer

Length: 645 bp
ATGGATGCCCTGAAAGGCAAGCCAGCCATGAAGAAATCCTCCTCTATCAACTCCGACGACTCCGCCTGCACCGCCGGCTCCCCCCTCCGCTACCTCTCCCCCATGCGATCCGACGCCGGCGACCCCTCCGAATCCCCGCCCTACGCCTCCCCGGAGCTCTCCCCCCAGAAGCAGCCGCCCCCCGACAACTCCAAGGCCATCATGGCCGTCGACAAGTTCACGCAGTACTCCCCCTCCCCCTTCCGCCCTCAGAAACCGCCCGAGAAGGCGCCCTCGCCGATCGTCCTCTACAACAGCAACCGGGCCGCCAGGGAGGACCCCCCGCCGCCCTCGGTGCCCAAGGCGGCCGCTCCTAACGGCGTGCCCTGGCCGGCCGGAGGCGGCGCCGGGGTTGGGAGATCGAGGCGGCCGTCGAGGAGAGCCGATGTGTTGACGACGGCGGCGCTAGGGTTTCGGGTTAGCGAGTTGGTGCTATGCTTGATTTCGTTCTCGGTTATGGCCGCTGATAAAACTCAGGGCTGGAGCGGCGATTCCTTTGATCGCTACAAGGAATACAGGTTTTCCATCTCTCTCTCTCTCTCTCTCTCTCTCTCAGTTTTGAATTCTCAATTGGAATTTCAAGTTTTGGCCAACAATTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

214

Amino Acids

22.93

Weight (kDa)

9.39

Isoelectric Point (pI)

68.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 143 - 190 2.5e-07 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 268, 334, 383
AccBSI CCGCTC 2 cut(s) 350, 525
AccI GTMKAC 1 cut(s) 210
AclWI GGATC 1 cut(s) 102
AcoI YGGCCR 7 cut(s) 204, 345, 368, 372, 407, 498, 627
AcsI RAATTY 2 cut(s) 601, 614
AcyI GRCGYC 3 cut(s) 114, 269, 384
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 377, 393
AgsI TTSAA 3 cut(s) 601, 620, 640
AjnI CCWGG 2 cut(s) 308, 365
AjuI GAANNNNNNNTTGG 2 cut(s) 593, 625
AluBI AGCT 1 cut(s) 157
AluI AGCT 1 cut(s) 157
Alw21I GWGCWC 1 cut(s) 159
AlwI GGATC 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 9 cut(s) 195, 204, 303, 345, 368, 372, 407, 498, 627
ApeKI GCWGC 1 cut(s) 172
ApoI RAATTY 2 cut(s) 601, 614
AspLEI GCGC 3 cut(s) 271, 386, 445
AspS9I GGNCC 2 cut(s) 303, 316
AsuC2I CCSGG 3 cut(s) 152, 302, 388
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 2 cut(s) 339, 366
BalI TGGCCA 1 cut(s) 629
BanI GGYRCC 3 cut(s) 268, 334, 383
BanII GRGCYC 1 cut(s) 159
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 184
BccI CCATC 2 cut(s) 206, 572
BceAI ACGGC 4 cut(s) 191, 373, 394, 453
BciT130I CCWGG 2 cut(s) 310, 367
BcnI CCSGG 3 cut(s) 152, 302, 388
BfaI CTAG 1 cut(s) 446
BfoI RGCGCY 3 cut(s) 272, 387, 446
BglI GCCNNNNNGGC 1 cut(s) 203
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 5 cut(s) 152, 302, 310, 367, 388
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 303, 316
BmiI GGNNCC 5 cut(s) 79, 270, 318, 336, 385
BmrFI CCNGG 5 cut(s) 152, 302, 310, 367, 388
BpmI CTGGAG 1 cut(s) 541
BpuMI CCSGG 3 cut(s) 152, 302, 388
BsaHI GRCGYC 3 cut(s) 114, 269, 384
BsaJI CCNNGG 7 cut(s) 150, 192, 309, 330, 339, 365, 387
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 393
Bse118I RCCGGY 3 cut(s) 74, 116, 370
BseBI CCWGG 2 cut(s) 310, 367
BseDI CCNNGG 7 cut(s) 150, 192, 309, 330, 339, 365, 387
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 377, 393
BseMII CTCAG 3 cut(s) 263, 527, 606
BseRI GAGGAG 2 cut(s) 31, 430
BseSI GKGCMC 2 cut(s) 339, 366
BseX3I CGGCCG 3 cut(s) 345, 372, 407
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 52
Bsh1285I CGRYCG 4 cut(s) 282, 348, 375, 410
BshFI GGCC 9 cut(s) 197, 206, 305, 347, 370, 374, 409, 500, 629
BshNI GGYRCC 3 cut(s) 268, 334, 383
BsiEI CGRYCG 4 cut(s) 282, 348, 375, 410
BsiHKAI GWGCWC 1 cut(s) 159
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 7 cut(s) 75, 117, 152, 301, 371, 375, 387
BslI CCNNNNNNNGG 2 cut(s) 377, 393
BsnI GGCC 9 cut(s) 197, 206, 305, 347, 370, 374, 409, 500, 629
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 3 cut(s) 159, 339, 366
Bsp143I GATC 4 cut(s) 107, 279, 398, 538
BspANI GGCC 9 cut(s) 197, 206, 305, 347, 370, 374, 409, 500, 629
BspCNI CTCAG 3 cut(s) 262, 526, 605
BspLI GGNNCC 5 cut(s) 79, 270, 318, 336, 385
BspPI GGATC 1 cut(s) 102
BspT107I GGYRCC 3 cut(s) 268, 334, 383
BsrBI CCGCTC 2 cut(s) 350, 525
BsrFI RCCGGY 3 cut(s) 74, 116, 370
BssAI RCCGGY 3 cut(s) 74, 116, 370
BssECI CCNNGG 7 cut(s) 150, 192, 309, 330, 339, 365, 387
BssMI GATC 4 cut(s) 107, 279, 398, 538
BssNI GRCGYC 3 cut(s) 114, 269, 384
BssT1I CCWWGG 2 cut(s) 192, 339
Bst2UI CCWGG 2 cut(s) 310, 367
BstACI GRCGYC 3 cut(s) 114, 269, 384
BstC8I GCNNGC 7 cut(s) 20, 24, 67, 76, 118, 362, 372
BstDEI CTNAG 3 cut(s) 249, 513, 592
BstF5I GGATG 1 cut(s) 10
BstH2I RGCGCY 3 cut(s) 272, 387, 446
BstHHI GCGC 3 cut(s) 271, 386, 445
BstKTI GATC 4 cut(s) 110, 282, 401, 541
BstMBI GATC 4 cut(s) 107, 279, 398, 538
BstMCI CGRYCG 4 cut(s) 282, 348, 375, 410
BstMWI GCNNNNNNNGC 5 cut(s) 71, 75, 203, 334, 525
BstNI CCWGG 2 cut(s) 310, 367
BstSCI CCNGG 5 cut(s) 150, 300, 308, 365, 386
BstSLI GKGCMC 2 cut(s) 339, 366
BstV1I GCAGC 1 cut(s) 184
BstZI CGGCCG 3 cut(s) 345, 372, 407
BsuRI GGCC 9 cut(s) 197, 206, 305, 347, 370, 374, 409, 500, 629
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 7 cut(s) 20, 24, 67, 76, 118, 362, 372
CciNI GCGGCCGC 1 cut(s) 345
CfoI GCGC 3 cut(s) 271, 386, 445
Cfr10I RCCGGY 3 cut(s) 74, 116, 370
Cfr13I GGNCC 2 cut(s) 303, 316
CseI GACGC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 226
CviAII CATG 3 cut(s) 28, 103, 202
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 3 cut(s) 249, 513, 592
DinI GGCGCC 2 cut(s) 270, 385
DpnI GATC 4 cut(s) 109, 281, 400, 540
DpnII GATC 4 cut(s) 107, 279, 398, 538
EaeI YGGCCR 7 cut(s) 204, 345, 368, 372, 407, 498, 627
EagI CGGCCG 3 cut(s) 345, 372, 407
EciI GGCGGA 2 cut(s) 52, 233
Ecl136II GAGCTC 1 cut(s) 157
EclXI CGGCCG 3 cut(s) 345, 372, 407
Eco130I CCWWGG 2 cut(s) 192, 339
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 316
Eco52I CGGCCG 3 cut(s) 345, 372, 407
Eco53kI GAGCTC 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 2 cut(s) 308, 365
EcoT14I CCWWGG 2 cut(s) 192, 339
EcoT38I GRGCYC 1 cut(s) 159
EgeI GGCGCC 2 cut(s) 270, 385
EheI GGCGCC 2 cut(s) 270, 385
ErhI CCWWGG 2 cut(s) 192, 339
FaeI CATG 3 cut(s) 31, 106, 205
FaiI YATR 5 cut(s) 29, 104, 203, 475, 497
FatI CATG 3 cut(s) 27, 102, 201
FauI CCCGC 2 cut(s) 144, 330
FblI GTMKAC 1 cut(s) 210
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 159
FseI GGCCGGCC 1 cut(s) 374
FspBI CTAG 1 cut(s) 446
GlaI GCGC 3 cut(s) 270, 385, 444
GsuI CTGGAG 1 cut(s) 541
HaeII RGCGCY 3 cut(s) 272, 387, 446
HaeIII GGCC 9 cut(s) 197, 206, 305, 347, 370, 374, 409, 500, 629
HapII CCGG 7 cut(s) 75, 117, 152, 301, 371, 375, 387
HgaI GACGC 1 cut(s) 122
HhaI GCGC 3 cut(s) 271, 386, 445
Hin1I GRCGYC 3 cut(s) 114, 269, 384
Hin1II CATG 3 cut(s) 31, 106, 205
Hin6I GCGC 3 cut(s) 269, 384, 443
HinP1I GCGC 3 cut(s) 269, 384, 443
HincII GTYRAC 2 cut(s) 211, 432
HindII GTYRAC 2 cut(s) 211, 432
HinfI GANTC 3 cut(s) 59, 131, 530
HpaII CCGG 7 cut(s) 75, 117, 152, 301, 371, 375, 387
Hpy166II GTNNAC 3 cut(s) 211, 219, 432
Hpy188I TCNGA 4 cut(s) 55, 112, 130, 252
Hpy8I GTNNAC 3 cut(s) 211, 219, 432
Hpy99I CGWCG 5 cut(s) 59, 116, 212, 415, 439
HpyAV CCTTC 2 cut(s) 250, 259
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 75, 203, 334, 525
HpyF3I CTNAG 3 cut(s) 249, 513, 592
Hsp92I GRCGYC 3 cut(s) 114, 269, 384
Hsp92II CATG 3 cut(s) 31, 106, 205
HspAI GCGC 3 cut(s) 269, 384, 443
KasI GGCGCC 2 cut(s) 268, 383
KroI GCCGGC 3 cut(s) 74, 116, 370
KroNI GCCGGC 3 cut(s) 76, 118, 372
Kzo9I GATC 4 cut(s) 107, 279, 398, 538
LmnI GCTCC 4 cut(s) 83, 154, 355, 522
Lsp1109I GCAGC 1 cut(s) 184
MaeI CTAG 1 cut(s) 446
MalI GATC 4 cut(s) 109, 281, 400, 540
MbiI CCGCTC 2 cut(s) 350, 525
MboI GATC 4 cut(s) 107, 279, 398, 538
MboII GAAGA 1 cut(s) 43
MfeI CAATTG 1 cut(s) 608
MhlI GDGCHC 3 cut(s) 159, 339, 366
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 5 cut(s) 601, 608, 614, 634, 640
MluNI TGGCCA 1 cut(s) 629
Mly113I GGCGCC 2 cut(s) 269, 384
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 2 cut(s) 78, 135
Mox20I TGGCCA 1 cut(s) 629
MreI CGCCGGCG 1 cut(s) 116
MroNI GCCGGC 3 cut(s) 74, 116, 370
MscI TGGCCA 1 cut(s) 629
MseI TTAA 1 cut(s) 643
Msp20I TGGCCA 1 cut(s) 629
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 7 cut(s) 75, 117, 152, 301, 371, 375, 387
MspR9I CCNGG 5 cut(s) 152, 302, 310, 367, 388
MunI CAATTG 1 cut(s) 608
MvaI CCWGG 2 cut(s) 310, 367
MwoI GCNNNNNNNGC 5 cut(s) 71, 75, 203, 334, 525
NaeI GCCGGC 3 cut(s) 76, 118, 372
NarI GGCGCC 2 cut(s) 269, 384
NciI CCSGG 3 cut(s) 152, 302, 388
NdeII GATC 4 cut(s) 107, 279, 398, 538
NgoMIV GCCGGC 3 cut(s) 74, 116, 370
NlaIII CATG 3 cut(s) 31, 106, 205
NlaIV GGNNCC 5 cut(s) 79, 270, 318, 336, 385
NotI GCGGCCGC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PdiI GCCGGC 3 cut(s) 76, 118, 372
PfeI GAWTC 2 cut(s) 131, 530
Ple19I CGATCG 1 cut(s) 282
PleI GAGTC 1 cut(s) 53
PluTI GGCGCC 2 cut(s) 272, 387
PpsI GAGTC 1 cut(s) 53
PpuMI RGGWCCY 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 2 cut(s) 308, 365
PspGI CCWGG 2 cut(s) 308, 365
PspN4I GGNNCC 5 cut(s) 79, 270, 318, 336, 385
PspPI GGNCC 2 cut(s) 303, 316
PspPPI RGGWCCY 1 cut(s) 316
PvuI CGATCG 1 cut(s) 282
RigI GGCCGGCC 1 cut(s) 374
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SacI GAGCTC 1 cut(s) 159
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 1 cut(s) 643
Sau3AI GATC 4 cut(s) 107, 279, 398, 538
Sau96I GGNCC 2 cut(s) 303, 316
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 5 cut(s) 152, 302, 310, 367, 388
SduI GDGCHC 3 cut(s) 159, 339, 366
SetI ASST 3 cut(s) 96, 159, 560
SfiI GGCCNNNNNGGCC 1 cut(s) 203
SfoI GGCGCC 2 cut(s) 270, 385
SgrAI CRCCGGYG 1 cut(s) 116
SinI GGWCC 1 cut(s) 316
Sse9I AATT 5 cut(s) 601, 608, 614, 634, 640
SspDI GGCGCC 2 cut(s) 268, 383
SspMI CTAG 1 cut(s) 446
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 5 cut(s) 150, 300, 308, 365, 386
StyI CCWWGG 2 cut(s) 192, 339
TaqI TCGA 3 cut(s) 210, 401, 413
TasI AATT 5 cut(s) 601, 608, 614, 634, 640
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 2 cut(s) 131, 530
Tru1I TTAA 1 cut(s) 643
Tru9I TTAA 1 cut(s) 643
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 2 cut(s) 601, 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 1 cut(s) 446
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.