Rroxscaffold_3G00261910

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006012
Physical Location & Seq
Forward (+)
56049507 .. 56051513
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00261910.1

Sequence Viewer

Length: 852 bp
ATGGATGCCCTGAAAGGCAAGCCAGCCATGAAGAAATCCTCCTCCATCAACTCCGACGACTCCGCCTGCACCGCCGGCTCCCCCTCCGCTACCTCTCCCCATGCGATCCGACGCCGGCGACCCCTCCGAATCCCCGCCCTACGCCTCCCCGGAGCTCTCCCCCAGAAGCCGCCCCCCCGACAACTCCAAGGCCATCATGGCCGTCGACAAGTTCACGCAGTACTCCCCCTCCCCCTTCCGCCCTCAGAAACCGCCCGAGAAGGCGCCCTCGCCGATCGTCGTCTACAACAGCAACCGCGCCGCCAGGGAGGACCCCCGCCCTCCGTGCCCAAGGCGGCCGCTCCTAACGGCGTGCCCTGGCCGGCTGGAGGCGGCGCCGGGGTTGGCAGATCGAGGCGGCCGCCGAGGAGAGACGATGTGTTGACGACGGCGGCGCTAGGGTTTCGGTTACAACTTGAAACCGATTTGATTGCTCTGATTGATTTAGGGAGTTCAAATGGTTGGATTAGCAAAAATGAAAAGTATTGTTTAGCTGTGACTGTTATTGGATTTGCATATGCCGCTTTTCAAGCATACAATCTATCCTACCAGCTAGTCACCGGGAATTATGTGATCAGGCACCACCTTCGTCACCACTTTGATTTCTTCATGGATCAGGTACTGGCATATCTTCTCATTTCAGCATCATCATCGGCAGCCACCAGGGTCGACGACTGGCAATCAAATTGGGGAAAAGATGAGTTCACGGAGATGGCTACTGCTTCAGTCAGCATGGCCTTCCTCGCTTTCATTGCCTTTGCACTAAGCTCCCTGATCTCTGGTTACAACCTATGTACCCATGATTCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

283

Amino Acids

30.7

Weight (kDa)

10.0

Isoelectric Point (pI)

65.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 174 - 265 1.1e-17 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 263, 374, 618
AccBSI CCGCTC 1 cut(s) 341
AccI GTMKAC 3 cut(s) 205, 283, 708
AccII CGCG 1 cut(s) 298
AclWI GGATC 2 cut(s) 100, 660
AcoI YGGCCR 4 cut(s) 199, 336, 359, 398
AcuI CTGAAG 1 cut(s) 747
AcyI GRCGYC 3 cut(s) 112, 264, 375
AfaI GTAC 3 cut(s) 222, 660, 835
AfiI CCNNNNNNNGG 1 cut(s) 368
AgsI TTSAA 3 cut(s) 458, 495, 569
AjnI CCWGG 3 cut(s) 303, 356, 701
AluBI AGCT 4 cut(s) 155, 533, 592, 807
AluI AGCT 4 cut(s) 155, 533, 592, 807
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 1 cut(s) 405
AlwI GGATC 2 cut(s) 100, 660
AlwNI CAGNNNCTG 1 cut(s) 661
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 6 cut(s) 190, 199, 336, 359, 398, 774
ApeKI GCWGC 1 cut(s) 695
AspLEI GCGC 4 cut(s) 266, 300, 377, 436
AspS9I GGNCC 1 cut(s) 311
AsuC2I CCSGG 3 cut(s) 150, 379, 601
AsuHPI GGTGA 2 cut(s) 589, 623
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 1 cut(s) 311
BaeGI GKGCMC 2 cut(s) 330, 357
BaeI ACNNNNGTAYC 2 cut(s) 817, 850
BanI GGYRCC 3 cut(s) 263, 374, 618
BanII GRGCYC 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 1 cut(s) 707
BccI CCATC 3 cut(s) 53, 201, 745
BceAI ACGGC 3 cut(s) 186, 364, 444
BcgI CGANNNNNNTGC 6 cut(s) 452, 486, 608, 642, 672, 706
BciT130I CCWGG 3 cut(s) 305, 358, 703
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 3 cut(s) 150, 379, 601
BcoDI GTCTC 1 cut(s) 405
BfaI CTAG 2 cut(s) 437, 593
BfoI RGCGCY 3 cut(s) 267, 378, 437
BglI GCCNNNNNGGC 1 cut(s) 198
BmcAI AGTACT 1 cut(s) 222
Bme1390I CCNGG 6 cut(s) 150, 305, 358, 379, 601, 703
Bme18I GGWCC 1 cut(s) 311
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 5 cut(s) 79, 265, 313, 376, 620
BmrFI CCNGG 6 cut(s) 150, 305, 358, 379, 601, 703
BmsI GCATC 1 cut(s) 692
BpmI CTGGAG 1 cut(s) 387
BpuMI CCSGG 3 cut(s) 150, 379, 601
BsaAI YACGTR 1 cut(s) 849
BsaHI GRCGYC 3 cut(s) 112, 264, 375
BsaJI CCNNGG 8 cut(s) 148, 187, 304, 330, 356, 378, 404, 702
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 1 cut(s) 368
Bse118I RCCGGY 3 cut(s) 74, 114, 361
Bse1I ACTGG 2 cut(s) 666, 719
Bse3DI GCAATG 1 cut(s) 789
BseBI CCWGG 3 cut(s) 305, 358, 703
BseDI CCNNGG 8 cut(s) 148, 187, 304, 330, 356, 378, 404, 702
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 368
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 2 cut(s) 666, 719
BseRI GAGGAG 2 cut(s) 31, 421
BseSI GKGCMC 2 cut(s) 330, 357
BseX3I CGGCCG 2 cut(s) 336, 398
BseXI GCAGC 1 cut(s) 707
BsgI GTGCAG 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 298
Bsh1285I CGRYCG 3 cut(s) 277, 339, 401
BshFI GGCC 6 cut(s) 192, 201, 338, 361, 400, 776
BshNI GGYRCC 3 cut(s) 263, 374, 618
BsiEI CGRYCG 3 cut(s) 277, 339, 401
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 6 cut(s) 75, 115, 150, 362, 378, 600
BslI CCNNNNNNNGG 1 cut(s) 368
BsmAI GTCTC 1 cut(s) 405
BsmBI CGTCTC 1 cut(s) 405
BsnI GGCC 6 cut(s) 192, 201, 338, 361, 400, 776
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 3 cut(s) 157, 330, 357
Bsp143I GATC 6 cut(s) 105, 274, 389, 612, 652, 813
BspANI GGCC 6 cut(s) 192, 201, 338, 361, 400, 776
BspCNI CTCAG 1 cut(s) 257
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 5 cut(s) 79, 265, 313, 376, 620
BspPI GGATC 2 cut(s) 100, 660
BspT107I GGYRCC 3 cut(s) 263, 374, 618
BsrBI CCGCTC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 789
BsrFI RCCGGY 3 cut(s) 74, 114, 361
BsrI ACTGG 2 cut(s) 666, 719
BssAI RCCGGY 3 cut(s) 74, 114, 361
BssECI CCNNGG 8 cut(s) 148, 187, 304, 330, 356, 378, 404, 702
BssMI GATC 6 cut(s) 105, 274, 389, 612, 652, 813
BssNI GRCGYC 3 cut(s) 112, 264, 375
BssT1I CCWWGG 2 cut(s) 187, 330
Bst2UI CCWGG 3 cut(s) 305, 358, 703
Bst4CI ACNGT 1 cut(s) 541
BstACI GRCGYC 3 cut(s) 112, 264, 375
BstBAI YACGTR 1 cut(s) 849
BstC8I GCNNGC 7 cut(s) 20, 24, 67, 76, 116, 353, 363
BstDEI CTNAG 2 cut(s) 244, 803
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 298
BstH2I RGCGCY 3 cut(s) 267, 378, 437
BstHHI GCGC 4 cut(s) 266, 300, 377, 436
BstKTI GATC 6 cut(s) 108, 277, 392, 615, 655, 816
BstMAI GTCTC 1 cut(s) 405
BstMBI GATC 6 cut(s) 105, 274, 389, 612, 652, 813
BstMCI CGRYCG 3 cut(s) 277, 339, 401
BstMWI GCNNNNNNNGC 8 cut(s) 71, 75, 198, 325, 560, 569, 782, 791
BstNI CCWGG 3 cut(s) 305, 358, 703
BstSCI CCNGG 6 cut(s) 148, 303, 356, 377, 599, 701
BstSLI GKGCMC 2 cut(s) 330, 357
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 1 cut(s) 707
BstZI CGGCCG 2 cut(s) 336, 398
BsuRI GGCC 6 cut(s) 192, 201, 338, 361, 400, 776
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 7 cut(s) 20, 24, 67, 76, 116, 353, 363
CaiI CAGNNNCTG 1 cut(s) 661
CciNI GCGGCCGC 2 cut(s) 336, 398
CfoI GCGC 4 cut(s) 266, 300, 377, 436
Cfr10I RCCGGY 3 cut(s) 74, 114, 361
Cfr13I GGNCC 1 cut(s) 311
CseI GACGC 1 cut(s) 120
Csp6I GTAC 3 cut(s) 221, 659, 834
CviAII CATG 6 cut(s) 28, 101, 197, 649, 772, 839
CviQI GTAC 3 cut(s) 221, 659, 834
DdeI CTNAG 2 cut(s) 244, 803
DinI GGCGCC 2 cut(s) 265, 376
DpnI GATC 6 cut(s) 107, 276, 391, 614, 654, 815
DpnII GATC 6 cut(s) 105, 274, 389, 612, 652, 813
EaeI YGGCCR 4 cut(s) 199, 336, 359, 398
EagI CGGCCG 2 cut(s) 336, 398
EciI GGCGGA 2 cut(s) 52, 228
Ecl136II GAGCTC 1 cut(s) 155
EclXI CGGCCG 2 cut(s) 336, 398
Eco130I CCWWGG 2 cut(s) 187, 330
Eco24I GRGCYC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 311
Eco52I CGGCCG 2 cut(s) 336, 398
Eco53kI GAGCTC 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 747
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 311
EcoRII CCWGG 3 cut(s) 303, 356, 701
EcoT14I CCWWGG 2 cut(s) 187, 330
EcoT38I GRGCYC 1 cut(s) 157
EgeI GGCGCC 2 cut(s) 265, 376
EheI GGCGCC 2 cut(s) 265, 376
ErhI CCWWGG 2 cut(s) 187, 330
Esp3I CGTCTC 1 cut(s) 405
FaeI CATG 6 cut(s) 31, 104, 200, 652, 775, 842
FatI CATG 6 cut(s) 27, 100, 196, 648, 771, 838
FauI CCCGC 2 cut(s) 142, 324
FauNDI CATATG 1 cut(s) 556
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 3 cut(s) 205, 283, 708
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 157
FspBI CTAG 2 cut(s) 437, 593
GlaI GCGC 4 cut(s) 265, 299, 376, 435
GsuI CTGGAG 1 cut(s) 387
HaeII RGCGCY 3 cut(s) 267, 378, 437
HaeIII GGCC 6 cut(s) 192, 201, 338, 361, 400, 776
HapII CCGG 6 cut(s) 75, 115, 150, 362, 378, 600
HgaI GACGC 1 cut(s) 120
HhaI GCGC 4 cut(s) 266, 300, 377, 436
Hin1I GRCGYC 3 cut(s) 112, 264, 375
Hin1II CATG 6 cut(s) 31, 104, 200, 652, 775, 842
Hin6I GCGC 4 cut(s) 264, 298, 375, 434
HinP1I GCGC 4 cut(s) 264, 298, 375, 434
HincII GTYRAC 3 cut(s) 206, 423, 709
HindII GTYRAC 3 cut(s) 206, 423, 709
HinfI GANTC 3 cut(s) 59, 129, 842
HpaII CCGG 6 cut(s) 75, 115, 150, 362, 378, 600
HphI GGTGA 2 cut(s) 589, 623
Hpy166II GTNNAC 6 cut(s) 206, 214, 284, 423, 709, 744
Hpy188I TCNGA 5 cut(s) 55, 110, 128, 247, 477
Hpy8I GTNNAC 6 cut(s) 206, 214, 284, 423, 709, 744
Hpy99I CGWCG 6 cut(s) 59, 114, 207, 282, 430, 713
HpyAV CCTTC 4 cut(s) 245, 254, 635, 787
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4IV ACGT 1 cut(s) 848
HpyCH4V TGCA 3 cut(s) 69, 554, 800
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 75, 198, 325, 560, 569, 782, 791
HpyF3I CTNAG 2 cut(s) 244, 803
HpySE526I ACGT 1 cut(s) 848
Hsp92I GRCGYC 3 cut(s) 112, 264, 375
Hsp92II CATG 6 cut(s) 31, 104, 200, 652, 775, 842
HspAI GCGC 4 cut(s) 264, 298, 375, 434
KasI GGCGCC 2 cut(s) 263, 374
KroI GCCGGC 3 cut(s) 74, 114, 361
KroNI GCCGGC 3 cut(s) 76, 116, 363
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 6 cut(s) 105, 274, 389, 612, 652, 813
LmnI GCTCC 4 cut(s) 83, 152, 346, 812
Lsp1109I GCAGC 1 cut(s) 707
LweI GCATC 1 cut(s) 692
MaeI CTAG 2 cut(s) 437, 593
MaeII ACGT 1 cut(s) 848
MaeIII GTNAC 5 cut(s) 447, 535, 595, 629, 821
MalI GATC 6 cut(s) 107, 276, 391, 614, 654, 815
MbiI CCGCTC 1 cut(s) 341
MboI GATC 6 cut(s) 105, 274, 389, 612, 652, 813
MboII GAAGA 3 cut(s) 43, 637, 662
MhlI GDGCHC 3 cut(s) 157, 330, 357
MluCI AATT 2 cut(s) 604, 724
Mly113I GGCGCC 2 cut(s) 264, 375
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 3 cut(s) 78, 133, 482
MreI CGCCGGCG 1 cut(s) 114
MroNI GCCGGC 3 cut(s) 74, 114, 361
MslI CAYNNNNRTG 1 cut(s) 749
MspI CCGG 6 cut(s) 75, 115, 150, 362, 378, 600
MspR9I CCNGG 6 cut(s) 150, 305, 358, 379, 601, 703
MvaI CCWGG 3 cut(s) 305, 358, 703
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 8 cut(s) 71, 75, 198, 325, 560, 569, 782, 791
NaeI GCCGGC 3 cut(s) 76, 116, 363
NarI GGCGCC 2 cut(s) 264, 375
NciI CCSGG 3 cut(s) 150, 379, 601
NdeI CATATG 1 cut(s) 556
NdeII GATC 6 cut(s) 105, 274, 389, 612, 652, 813
NgoMIV GCCGGC 3 cut(s) 74, 114, 361
NlaIII CATG 6 cut(s) 31, 104, 200, 652, 775, 842
NlaIV GGNNCC 5 cut(s) 79, 265, 313, 376, 620
NmeAIII GCCGAG 1 cut(s) 429
NmuCI GTSAC 3 cut(s) 535, 595, 629
NotI GCGGCCGC 2 cut(s) 336, 398
PdiI GCCGGC 3 cut(s) 76, 116, 363
PfeI GAWTC 2 cut(s) 129, 842
Ple19I CGATCG 1 cut(s) 277
PleI GAGTC 1 cut(s) 53
PluTI GGCGCC 2 cut(s) 267, 378
PpsI GAGTC 1 cut(s) 53
Ppu21I YACGTR 1 cut(s) 849
PpuMI RGGWCCY 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 311
Psp6I CCWGG 3 cut(s) 303, 356, 701
PspGI CCWGG 3 cut(s) 303, 356, 701
PspN4I GGNNCC 5 cut(s) 79, 265, 313, 376, 620
PspPI GGNCC 1 cut(s) 311
PspPPI RGGWCCY 1 cut(s) 311
PstNI CAGNNNCTG 1 cut(s) 661
PvuI CGATCG 1 cut(s) 277
RsaI GTAC 3 cut(s) 222, 660, 835
RsaNI GTAC 3 cut(s) 221, 659, 834
RseI CAYNNNNRTG 1 cut(s) 749
SacI GAGCTC 1 cut(s) 157
SalI GTCGAC 2 cut(s) 204, 707
Sau3AI GATC 6 cut(s) 105, 274, 389, 612, 652, 813
Sau96I GGNCC 1 cut(s) 311
ScaI AGTACT 1 cut(s) 222
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 6 cut(s) 150, 305, 358, 379, 601, 703
SduI GDGCHC 3 cut(s) 157, 330, 357
SetI ASST 9 cut(s) 95, 157, 535, 594, 627, 660, 809, 831, 851
SfaNI GCATC 1 cut(s) 692
SfiI GGCCNNNNNGGCC 1 cut(s) 198
SfoI GGCGCC 2 cut(s) 265, 376
SgrAI CRCCGGYG 1 cut(s) 114
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 749
Sse9I AATT 2 cut(s) 604, 724
SspDI GGCGCC 2 cut(s) 263, 374
SspMI CTAG 2 cut(s) 437, 593
SstI GAGCTC 1 cut(s) 157
StyD4I CCNGG 6 cut(s) 148, 303, 356, 377, 599, 701
StyI CCWWGG 2 cut(s) 187, 330
TaaI ACNGT 1 cut(s) 541
TaiI ACGT 1 cut(s) 851
TaqI TCGA 3 cut(s) 205, 392, 708
TasI AATT 2 cut(s) 604, 724
TatI WGTACW 1 cut(s) 220
TauI GCSGC 9 cut(s) 172, 303, 338, 341, 375, 400, 403, 434, 563
TfiI GAWTC 2 cut(s) 129, 842
TseFI GTSAC 3 cut(s) 535, 595, 629
TseI GCWGC 1 cut(s) 695
Tsp45I GTSAC 3 cut(s) 535, 595, 629
TspDTI ATGAA 4 cut(s) 44, 531, 637, 778
TspGWI ACGGA 2 cut(s) 313, 761
VpaK11BI GGWCC 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmiI GTMKAC 3 cut(s) 205, 283, 708
XspI CTAG 2 cut(s) 437, 593
ZrmI AGTACT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.