FvH4_5g07280

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002691
Physical Location & Seq
Forward (+)
4258696 .. 4267992
9297 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07280.t3

Sequence Viewer

Length: 2979 bp
ATGGCTCACGAGTTTGAAGCTTGTGCAGAATGCACTCACAGGTGCCAGCTGGCTCATGGGAAAAAGAAAAGCGGTTCCCATATTGCCAACTCTTTTTTCAAAATCATGATTGGAAAACAGTTTTCTAAATTTATGGAGTTGCCTCCAAAAGTGGCTCGCATTGTGTTATTGTCTGATCGGAAAACTTTTCTTGAAGATTTAACCAGACAGCGGTGGAACGTTGCAATATCTACTGTTAATGGTTCCTTGGCCTTTCAACAAGGATGGAATGCTTTTGCATTAGACCATGACTTGCAAGTAGGAGATTTTCTGGTCTTCAATTACGTTGTGGGATCACATTTTACTGTTAAGATTTATGACAATTCTGGGTGTGAACTGAATCTTTTCGAAACCATACATCAGCACAACATAAATAGGGATCAGTGCCACACATTTGTTAAAAGTACAGCGAGCAAATCCGGTTTAGTCACCTTTGCTGGTCCAGATGCAGAAATAAGTAAATGCTTGAATGAACTGAATGGCATGAACAAGCCTCTGATTATCACAGAATATGCTTCAACTCATGATGATGATAATGAGAGGTCAAAGGGCAAGCGCAAAGCAGAATTTGCTGAAGACATGTCATGTATGATGAAAAGAGATTATGGAAACCAACCAGAAGAGAACAGAGCCCATATTCTCGATTTGTCTAGTGTTGAATCGTTCAAAAAGATAAGAACCTGTGGCAGTGATGGAACTAAAGTTTCTGTAGTGAATGAAACACATTCATATTGTGCTGATTTATCACTCAAATTGAGAAGTGAAGCTGACTCGGTAAAAATCCCAGTGGCTAAAGAGGTACCGAATAGAGTAATTCCTTTGGATGCTGCTGAGCTTGAAAGGACTGAGAAAAATAACTATTCTGAATCCAAGGTAAAGGGGGCATCCCTGTCTGATAAAGATTCATCCACCAATGTGACCTCGGGACATCTATTTCCAACTCTTCCTGATAACAATCAACAAAATGATATCTTCTCTGGTACTTTGATTATAGCAGCCCAGAAATGCCATTCTGCTAATGCATCAGGTAATGATCATCAGGTAGCTGTATCAGGACAAGAAAATGATTTGATGGATGACACTTGTCAAAGTGCCAACAAGAATATTACAATTAGTGGAATTTCAAAGTCTTGTGATACAGCGATGGAGGTCATTGAAATTGAGCCTTTGGAGTTGACTTCACATCCATGCAGTAAGGAGGTCATTCCAGAAAGTAATGATGATGCGATGATCATTACTGAAAAAGAGTTTAAGGAGTTTAGTTCAGATCCATGCAGTCAGGAGGTCATTCCAGAAAGAAAGGATGAAACTACAAGAGCCATTGAAAATGTTATTGTGGACTTAACTTCAGATGAATGCAGAAAGCAGGTCATTGAAAAAGAGGAATCACCAGATGTTTCAGCCAAGGTGGATAATCAGAGTTCTAATAGCCAACGAAGAGATATTCCTTCAGATCAAGCTGAGTTTGAAAGGACTGAGGAACATAACAAGTCTGAAGCCAAGGTCAATAAAGTACCGTCTGATAAAGATTCATGCACTGAGACCTCTGGGCATCCCTTCCCAACACACTCCCAAACTCCTGAAGAAAATGGAGAGACCATCACTGATACTTCAAAGCCCACCTCTGATAATTCAAAGAAACCAACAAAAGAATGCCAGAATGCTGATGGATCAGAATTATTATTAAGCAATGATCAGGTGACACTAGTACAGATGGAACCTAATTTGATGGATGACAGCGTCCCAAGAGACAACAAGGAATTGACTGGAATTTCAGAGTCAATTCATGATCCCATTACTTTTGAAAGCCAAAATCTCTACGATACGATCATCAACGAGAAAGAGTCTCTTGAGGCGACTTGTCAAGAGGCCATTCCACAAAGAAAGGATACGATCATCATTGAAAGCGAGACTGTTGAGGTGACTTTAGAACCATGCAGTCAGGAGGTCATTCCAGAAATAAAGGAAACAATCATCATTGAAGATGGGTCTGCTGAAGTGACTTTAGATCCAAGCTGTCAGGAGACCATTCCAGAAAGAGAGAGGGAATCATTAATGAGTTTCAATAGGTCATACAGAAAGCGTGCTGATACTAAAATAAGAACAGCTTCTTCAGATGCATCTGACCTTGAAAGGACTGAGGAAAGTAATTATTCTGATGTTAAGGTCCGAAAGGTATCAACTTCTGATGAAGTACCACACACCAACCAGACCTCTTGTCATCTCAACCCAATATGTCCTGTAAATGTTGAAGAAGATACAACAAGCATTGCTAAGATTTCAAATGAAGGTTTAAACAAACTCAAGACTGCTGATAGATCAGTCATTTCAGGCAATGTTCAGGTGGCTGTATCCCAAAAGGAAAACGTTGTAATTGATGACAGCTGCCAACAAAAGAGATTTACTGGAGTTTCAAAGGTGTTTGAAGTTCCGGTTCCTTTTGGATCCCAAGATTATTCAGCACAACCATCGAAGATTTCTAACAAGTTGCAGAGCCAGAGTAATTTTGCCAGAGAAAATGAGACCACTCTTCCCGTGGTAAAATCTGAACCTGTTGAAGATGCAGTTGCAACCTTCCCAAGTTTCACGATTTCCTGTTTGGCAACAGACCGTCAATCTTATCTCGAGTTGCCAAAGAGCTTGCCAGTATCCTTGAAAGCCACGAACGGAAGGTCTAAAATGGATAGGAGGGTAGTTTATCTTCAAGGTCCCGATAACAGATTGTGGCCAGTTCTCTACATTCAGAAATTTTTCTTCAGAACTTTGGCAAATGGGTGGGAAGCTTTCAGCATGGCAAATAATATTGAAAAAGGAGATCGATGTACCATTGCGGTCATAGATCAAACTAAAGGCATATGTTCTGTGAACGTAGTAAAGATGAGAGATGATATGGAATTCTATGAAGAGTGGGAGCAGCAGCCTTTAATCAACCTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

993

Amino Acids

110.71

Weight (kDa)

4.92

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 32 - 121 6.3e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1396
Acc65I GGTACC 1 cut(s) 838
AccB1I GGYRCC 2 cut(s) 42, 838
AciI CCGC 3 cut(s) 72, 211, 2870
AclI AACGTT 2 cut(s) 219, 2406
AclWI GGATC 8 cut(s) 340, 426, 1301, 1717, 1823, 2042, 2478, 2491
AcoI YGGCCR 1 cut(s) 2765
AcsI RAATTY 6 cut(s) 128, 605, 1158, 1809, 2786, 2933
AcuI CTGAAG 8 cut(s) 633, 1371, 1473, 1554, 1641, 2055, 2136, 2779
AfaI GTAC 7 cut(s) 445, 840, 1021, 1554, 1749, 2235, 2863
AfiI CCNNNNNNNGG 1 cut(s) 210
AflIII ACRYGT 1 cut(s) 618
AhdI GACNNNNNGTC 1 cut(s) 2256
AhlI ACTAGT 1 cut(s) 1744
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AleI CACNNNNGTG 1 cut(s) 953
Alw26I GTCTC 7 cut(s) 1574, 1628, 1782, 1890, 1943, 2057, 2555
AlwI GGATC 8 cut(s) 340, 426, 1301, 1717, 1823, 2042, 2478, 2491
Ama87I CYCGRG 2 cut(s) 961, 2663
AoxI GGCC 3 cut(s) 249, 1908, 2765
ApeKI GCWGC 5 cut(s) 866, 1034, 2424, 2953, 2956
ApoI RAATTY 6 cut(s) 128, 605, 1158, 1809, 2786, 2933
ArsI GACNNNNNNTTYG 2 cut(s) 2696, 2728
AseI ATTAAT 1 cut(s) 2093
Asp700I GAANNNNTTC 3 cut(s) 383, 2146, 2789
Asp718I GGTACC 1 cut(s) 838
AspLEI GCGC 1 cut(s) 597
AspS9I GGNCC 3 cut(s) 479, 2206, 2747
AsuHPI GGTGA 4 cut(s) 460, 1419, 1750, 1972
AsuII TTCGAA 1 cut(s) 387
AvaI CYCGRG 2 cut(s) 961, 2663
AvaII GGWCC 3 cut(s) 479, 2206, 2747
BalI TGGCCA 1 cut(s) 2767
BamHI GGATCC 1 cut(s) 2483
BanI GGYRCC 2 cut(s) 42, 838
BanII GRGCYC 1 cut(s) 673
BarI GAAGNNNNNNTAC 2 cut(s) 2724, 2756
BauI CACGAG 1 cut(s) 8
BbsI GAAGAC 2 cut(s) 307, 621
BbvI GCAGC 5 cut(s) 853, 1046, 2411, 2965, 2968
BcgI CGANNNNNNTGC 2 cut(s) 2490, 2524
BciVI GTATCC 3 cut(s) 1921, 2401, 2698
BclI TGATCA 3 cut(s) 1072, 1269, 1732
BcoDI GTCTC 7 cut(s) 1574, 1628, 1782, 1890, 1943, 2057, 2555
BcuI ACTAGT 1 cut(s) 1744
BfaI CTAG 2 cut(s) 690, 1745
BfmI CTRYAG 1 cut(s) 747
BfuAI ACCTGC 1 cut(s) 1396
BfuI GTATCC 3 cut(s) 1921, 2401, 2698
BisI GCNGC 5 cut(s) 867, 1035, 2425, 2954, 2957
BlpI GCTNAGC 1 cut(s) 870
BlsI GCNGC 5 cut(s) 868, 1036, 2426, 2955, 2958
Bme18I GGWCC 3 cut(s) 479, 2206, 2747
BmeRI GACNNNNNGTC 1 cut(s) 2256
BmeT110I CYCGRG 2 cut(s) 961, 2663
BmgT120I GGNCC 3 cut(s) 479, 2206, 2747
BmiI GGNNCC 8 cut(s) 44, 76, 244, 840, 1758, 2475, 2485, 2749
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 9 cut(s) 475, 853, 932, 1070, 1252, 1600, 2146, 2168, 2590
BmuI ACTGGG 1 cut(s) 818
BoxI GACNNNNGTC 1 cut(s) 1122
BpiI GAAGAC 2 cut(s) 307, 621
BpmI CTGGAG 1 cut(s) 2466
Bpu1102I GCTNAGC 1 cut(s) 870
Bpu14I TTCGAA 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 1910, 2327
Bsa29I ATCGAT 1 cut(s) 2857
BsaBI GATNNNNATC 1 cut(s) 993
BsaI GGTCTC 4 cut(s) 1574, 1628, 2057, 2555
BsaJI CCNNGG 6 cut(s) 246, 909, 960, 1443, 1539, 2574
BsaWI WCCGGW 2 cut(s) 458, 2470
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse1I ACTGG 5 cut(s) 824, 1810, 2449, 2684, 2768
Bse3DI GCAATG 4 cut(s) 1735, 2307, 2380, 2865
Bse8I GATNNNNATC 1 cut(s) 993
BseCI ATCGAT 1 cut(s) 2857
BseDI CCNNGG 6 cut(s) 246, 909, 960, 1443, 1539, 2574
BseGI GGATG 9 cut(s) 269, 868, 923, 944, 1120, 1222, 1348, 1591, 1777
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 1 cut(s) 210
BseMI GCAATG 4 cut(s) 1735, 2307, 2380, 2865
BseMII CTCAG 6 cut(s) 861, 876, 1491, 1506, 1569, 2169
BseNI ACTGG 5 cut(s) 824, 1810, 2449, 2684, 2768
BseXI GCAGC 5 cut(s) 853, 1046, 2411, 2965, 2968
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 3 cut(s) 251, 1910, 2767
BshNI GGYRCC 2 cut(s) 42, 838
BshVI ATCGAT 1 cut(s) 2857
BsiHKCI CYCGRG 2 cut(s) 961, 2663
BsiSI CCGG 2 cut(s) 459, 2471
BslFI GGGAC 3 cut(s) 978, 1766, 2733
BslI CCNNNNNNNGG 1 cut(s) 210
BsmAI GTCTC 7 cut(s) 1574, 1628, 1782, 1890, 1943, 2057, 2555
BsmFI GGGAC 3 cut(s) 978, 1766, 2733
BsmI GAATGC 5 cut(s) 35, 274, 1400, 1697, 1705
BsnI GGCC 3 cut(s) 251, 1910, 2767
Bso31I GGTCTC 4 cut(s) 1574, 1628, 2057, 2555
BsoBI CYCGRG 2 cut(s) 961, 2663
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 673
Bsp1720I GCTNAGC 1 cut(s) 870
BspACI CCGC 3 cut(s) 72, 211, 2870
BspANI GGCC 3 cut(s) 251, 1910, 2767
BspCNI CTCAG 6 cut(s) 862, 877, 1492, 1507, 1570, 2170
BspDI ATCGAT 1 cut(s) 2857
BspHI TCATGA 3 cut(s) 105, 562, 1825
BspLI GGNNCC 8 cut(s) 44, 76, 244, 840, 1758, 2475, 2485, 2749
BspMI ACCTGC 1 cut(s) 1396
BspPI GGATC 8 cut(s) 340, 426, 1301, 1717, 1823, 2042, 2478, 2491
BspT104I TTCGAA 1 cut(s) 387
BspT107I GGYRCC 2 cut(s) 42, 838
BspTNI GGTCTC 4 cut(s) 1574, 1628, 2057, 2555
BsrDI GCAATG 4 cut(s) 1735, 2307, 2380, 2865
BsrI ACTGG 5 cut(s) 824, 1810, 2449, 2684, 2768
BssECI CCNNGG 6 cut(s) 246, 909, 960, 1443, 1539, 2574
BssSI CACGAG 1 cut(s) 8
BssT1I CCWWGG 4 cut(s) 246, 909, 1443, 1539
Bst2BI CACGAG 1 cut(s) 8
Bst4CI ACNGT 6 cut(s) 120, 235, 346, 1557, 1954, 2651
Bst6I CTCTTC 5 cut(s) 654, 987, 1471, 2574, 2937
BstAPI GCANNNNNTGC 1 cut(s) 608
BstBI TTCGAA 1 cut(s) 387
BstC8I GCNNGC 7 cut(s) 47, 51, 157, 451, 593, 2124, 2681
BstDEI CTNAG 7 cut(s) 870, 885, 1500, 1515, 1578, 2178, 2313
BstDSI CCRYGG 1 cut(s) 2574
BstF5I GGATG 9 cut(s) 269, 868, 923, 944, 1120, 1222, 1348, 1591, 1777
BstHHI GCGC 1 cut(s) 597
BstMAI GTCTC 7 cut(s) 1574, 1628, 1782, 1890, 1943, 2057, 2555
BstMWI GCNNNNNNNGC 1 cut(s) 608
BstNSI RCATGY 1 cut(s) 622
BstPAI GACNNNNGTC 1 cut(s) 1122
BstSFI CTRYAG 1 cut(s) 747
BstV1I GCAGC 5 cut(s) 853, 1046, 2411, 2965, 2968
BstV2I GAAGAC 2 cut(s) 307, 621
BstX2I RGATCY 3 cut(s) 1306, 2047, 2483
BstYI RGATCY 3 cut(s) 1306, 2047, 2483
Bsu15I ATCGAT 1 cut(s) 2857
BsuI GTATCC 3 cut(s) 1921, 2401, 2698
BsuRI GGCC 3 cut(s) 251, 1910, 2767
BsuTUI ATCGAT 1 cut(s) 2857
BtgI CCRYGG 1 cut(s) 2574
BtgZI GCGATG 2 cut(s) 1196, 1280
BtsCI GGATG 9 cut(s) 269, 868, 923, 944, 1120, 1222, 1348, 1591, 1777
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 5 cut(s) 428, 733, 831, 1575, 1641
BveI ACCTGC 1 cut(s) 1396
Cac8I GCNNGC 7 cut(s) 47, 51, 157, 451, 593, 2124, 2681
CciI TCATGA 3 cut(s) 105, 562, 1825
CfoI GCGC 1 cut(s) 597
Cfr13I GGNCC 3 cut(s) 479, 2206, 2747
ClaI ATCGAT 1 cut(s) 2857
CseI GACGC 1 cut(s) 1768
Csp6I GTAC 7 cut(s) 444, 839, 1020, 1553, 1748, 2234, 2862
CviQI GTAC 7 cut(s) 444, 839, 1020, 1553, 1748, 2234, 2862
DdeI CTNAG 7 cut(s) 870, 885, 1500, 1515, 1578, 2178, 2313
DraI TTTAAA 1 cut(s) 2334
DriI GACNNNNNGTC 1 cut(s) 2256
EaeI YGGCCR 1 cut(s) 2765
Eam1104I CTCTTC 5 cut(s) 654, 987, 1471, 2574, 2937
Eam1105I GACNNNNNGTC 1 cut(s) 2256
EarI CTCTTC 5 cut(s) 654, 987, 1471, 2574, 2937
Eco130I CCWWGG 4 cut(s) 246, 909, 1443, 1539
Eco24I GRGCYC 1 cut(s) 673
Eco31I GGTCTC 4 cut(s) 1574, 1628, 2057, 2555
Eco32I GATATC 1 cut(s) 1009
Eco47I GGWCC 3 cut(s) 479, 2206, 2747
Eco57I CTGAAG 8 cut(s) 633, 1371, 1473, 1554, 1641, 2055, 2136, 2779
Eco88I CYCGRG 2 cut(s) 961, 2663
EcoO109I RGGNCCY 1 cut(s) 2747
EcoRI GAATTC 1 cut(s) 2933
EcoRV GATATC 1 cut(s) 1009
EcoT14I CCWWGG 4 cut(s) 246, 909, 1443, 1539
EcoT22I ATGCAT 2 cut(s) 1063, 2161
EcoT38I GRGCYC 1 cut(s) 673
ErhI CCWWGG 4 cut(s) 246, 909, 1443, 1539
FalI AAGNNNNNCTT 4 cut(s) 1872, 1904, 2131, 2163
FaqI GGGAC 3 cut(s) 978, 1766, 2733
FauNDI CATATG 1 cut(s) 2894
FbaI TGATCA 3 cut(s) 1072, 1269, 1732
Fnu4HI GCNGC 5 cut(s) 867, 1035, 2425, 2954, 2957
FokI GGATG 9 cut(s) 276, 875, 910, 931, 1127, 1209, 1355, 1578, 1784
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 5 cut(s) 867, 1035, 2425, 2954, 2957
FspBI CTAG 2 cut(s) 690, 1745
GlaI GCGC 1 cut(s) 596
GluI GCNGC 5 cut(s) 867, 1035, 2425, 2954, 2957
GsuI CTGGAG 1 cut(s) 2466
HaeIII GGCC 3 cut(s) 251, 1910, 2767
HapII CCGG 2 cut(s) 459, 2471
HgaI GACGC 1 cut(s) 1768
HhaI GCGC 1 cut(s) 597
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HincII GTYRAC 1 cut(s) 1215
HindII GTYRAC 1 cut(s) 1215
HindIII AAGCTT 2 cut(s) 18, 2820
HpaII CCGG 2 cut(s) 459, 2471
HphI GGTGA 4 cut(s) 460, 1419, 1750, 1972
Hpy166II GTNNAC 4 cut(s) 374, 1215, 1378, 2905
Hpy8I GTNNAC 4 cut(s) 374, 1215, 1378, 2905
HpyAV CCTTC 5 cut(s) 1497, 1606, 2321, 2623, 2703
HpyCH4III ACNGT 6 cut(s) 120, 235, 346, 1557, 1954, 2651
HpyCH4IV ACGT 4 cut(s) 219, 324, 2406, 2907
HpyF10VI GCNNNNNNNGC 1 cut(s) 608
HpyF3I CTNAG 7 cut(s) 870, 885, 1500, 1515, 1578, 2178, 2313
HpySE526I ACGT 4 cut(s) 219, 324, 2406, 2907
HspAI GCGC 1 cut(s) 595
KpnI GGTACC 1 cut(s) 842
Ksp22I TGATCA 3 cut(s) 1072, 1269, 1732
LmnI GCTCC 1 cut(s) 2950
Lsp1109I GCAGC 5 cut(s) 853, 1046, 2411, 2965, 2968
LweI GCATC 9 cut(s) 475, 853, 932, 1070, 1252, 1600, 2146, 2168, 2590
MaeI CTAG 2 cut(s) 690, 1745
MaeII ACGT 4 cut(s) 219, 324, 2406, 2907
MaeIII GTNAC 5 cut(s) 466, 955, 1738, 1960, 2038
MflI RGATCY 3 cut(s) 1306, 2047, 2483
MhlI GDGCHC 1 cut(s) 673
MlsI TGGCCA 1 cut(s) 2767
MluNI TGGCCA 1 cut(s) 2767
MlyI GAGTC 3 cut(s) 803, 1826, 1892
MmeI TCCRAC 1 cut(s) 1001
Mox20I TGGCCA 1 cut(s) 2767
Mph1103I ATGCAT 2 cut(s) 1063, 2161
MroXI GAANNNNTTC 3 cut(s) 383, 2146, 2789
MscI TGGCCA 1 cut(s) 2767
MslI CAYNNNNRTG 3 cut(s) 567, 953, 1225
Msp20I TGGCCA 1 cut(s) 2767
MspA1I CMGCKG 3 cut(s) 49, 211, 2424
MspI CCGG 2 cut(s) 459, 2471
MssI GTTTAAAC 1 cut(s) 2334
Mva1269I GAATGC 5 cut(s) 35, 274, 1400, 1697, 1705
MwoI GCNNNNNNNGC 1 cut(s) 608
NdeI CATATG 1 cut(s) 2894
NlaIV GGNNCC 8 cut(s) 44, 76, 244, 840, 1758, 2475, 2485, 2749
NmuCI GTSAC 5 cut(s) 466, 955, 1738, 1960, 2038
NsiI ATGCAT 2 cut(s) 1063, 2161
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 387
OliI CACNNNNGTG 1 cut(s) 953
PaeR7I CTCGAG 1 cut(s) 2663
PagI TCATGA 3 cut(s) 105, 562, 1825
PciI ACATGT 1 cut(s) 618
PctI GAATGC 5 cut(s) 35, 274, 1400, 1697, 1705
PdmI GAANNNNTTC 3 cut(s) 383, 2146, 2789
PfeI GAWTC 7 cut(s) 379, 698, 905, 941, 1424, 1568, 2087
PflFI GACNNNGTC 1 cut(s) 1778
PkrI GCNGC 5 cut(s) 868, 1036, 2426, 2955, 2958
PleI GAGTC 3 cut(s) 803, 1825, 1891
PmeI GTTTAAAC 1 cut(s) 2334
PpsI GAGTC 3 cut(s) 803, 1825, 1891
PpuMI RGGWCCY 1 cut(s) 2747
PscI ACATGT 1 cut(s) 618
PshAI GACNNNNGTC 1 cut(s) 1122
PshBI ATTAAT 1 cut(s) 2093
Psp1406I AACGTT 2 cut(s) 219, 2406
Psp5II RGGWCCY 1 cut(s) 2747
PspN4I GGNNCC 8 cut(s) 44, 76, 244, 840, 1758, 2475, 2485, 2749
PspPI GGNCC 3 cut(s) 479, 2206, 2747
PspPPI RGGWCCY 1 cut(s) 2747
PsuI RGATCY 3 cut(s) 1306, 2047, 2483
PsyI GACNNNGTC 1 cut(s) 1778
PvuII CAGCTG 2 cut(s) 49, 2424
RsaI GTAC 7 cut(s) 445, 840, 1021, 1554, 1749, 2235, 2863
RsaNI GTAC 7 cut(s) 444, 839, 1020, 1553, 1748, 2234, 2862
RseI CAYNNNNRTG 3 cut(s) 567, 953, 1225
SatI GCNGC 5 cut(s) 867, 1035, 2425, 2954, 2957
Sau96I GGNCC 3 cut(s) 479, 2206, 2747
SchI GAGTC 3 cut(s) 803, 1826, 1892
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 9 cut(s) 475, 853, 932, 1070, 1252, 1600, 2146, 2168, 2590
SfcI CTRYAG 1 cut(s) 747
Sfr274I CTCGAG 1 cut(s) 2663
SfuI TTCGAA 1 cut(s) 387
SinI GGWCC 3 cut(s) 479, 2206, 2747
SlaI CTCGAG 1 cut(s) 2663
SmiMI CAYNNNNRTG 3 cut(s) 567, 953, 1225
SmlI CTYRAG 3 cut(s) 1889, 2342, 2663
SmoI CTYRAG 3 cut(s) 1889, 2342, 2663
SpeI ACTAGT 1 cut(s) 1744
SsiI CCGC 3 cut(s) 72, 211, 2870
SspI AATATT 2 cut(s) 1144, 2842
SspMI CTAG 2 cut(s) 690, 1745
StyI CCWWGG 4 cut(s) 246, 909, 1443, 1539
TaaI ACNGT 6 cut(s) 120, 235, 346, 1557, 1954, 2651
TaiI ACGT 4 cut(s) 222, 327, 2409, 2910
TaqI TCGA 5 cut(s) 387, 681, 2510, 2664, 2857
TatI WGTACW 2 cut(s) 443, 1747
TfiI GAWTC 7 cut(s) 379, 698, 905, 941, 1424, 1568, 2087
TscAI CASTG 5 cut(s) 428, 733, 831, 1582, 1648
TseFI GTSAC 5 cut(s) 466, 955, 1738, 1960, 2038
TseI GCWGC 5 cut(s) 866, 1034, 2424, 2953, 2956
Tsp45I GTSAC 5 cut(s) 466, 955, 1738, 1960, 2038
TspGWI ACGGA 1 cut(s) 2721
TspRI CASTG 5 cut(s) 428, 733, 831, 1582, 1648
Tth111I GACNNNGTC 1 cut(s) 1778
VpaK11BI GGWCC 3 cut(s) 479, 2206, 2747
VspI ATTAAT 1 cut(s) 2093
XapI RAATTY 6 cut(s) 128, 605, 1158, 1809, 2786, 2933
XceI RCATGY 1 cut(s) 622
XcmI CCANNNNNNNNNTGG 3 cut(s) 53, 1703, 2572
XhoI CTCGAG 1 cut(s) 2663
XmnI GAANNNNTTC 3 cut(s) 383, 2146, 2789
XspI CTAG 2 cut(s) 690, 1745
Zsp2I ATGCAT 2 cut(s) 1063, 2161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.