RLG00000004463

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002691
Physical Location & Seq
Forward (+)
59579974 .. 59588512
8539 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004463

Sequence Viewer

Length: 3138 bp
ATGGGGCCCAAAGCACCGGACAGAATCGGATTTCAAATTCCATTCCCCGTCGTGTCCAATAAGATAGAGTTGAAAGAGTTGTTGACAAGCTCAAAATCACTTAAAAGATCCAAGAGTTTCCTCTGGGTAGCAATAATCAACCGAGCACTGGAAGAGATGGTTCATGATTTCGAAGCTTGTGCAGATTGCACTCAGAATTGTTTGCTGATTCATGGGAATAAGAAAAGTGGTTCCCATATCGAAACCTCTTTTTTCAAAATCATGTTTGGAAAACAGTTTTCTAAATTTATGGAATTGCCTCCAAAAGTTGCTCGTATTTTGTTATTGTCTGATCGGAGAACTTTTCTTGAAGATTCACGTGGACTCCGGTGGAAAGTTGCAATATCTAGTGTTAATGGTTCCTGGGCCTTTCAACAAGGATGGAATGCTTTTGCACTAGACCATGACCTACAAGTAGGAGATTTTCTGGTCTTCAATTACGTTGTGGGTTCACATTTTACTGTTAAGATATATGACAACTCTGGGTGTGAAGTGAATCTTTTTGAGACCATACATCAGAACAACAGAAATAGGGTTCACAGAGATGGTCAGTGCCACACCATTGGTACAAGTACAGTGAGCAAATCCGTTTCAGATGCCTTTGCTGGTCCAGATGCAGAAATAAGTAAATGCTTGAATGAAGTGCATGGTATGAAGAAACCTCTAATTATCACAGAAAATGCTTCAGATCACGATGATGATTATGAAAGGTCAAAGTGCAAGCGCAAAGCAGAATTTGCTGACGACATGTCATGTATGATGGAAAGAGAATATGGACACCAACAAGGAGAGAACAGAGCCCGTATTCTTGATTTGTCTAGTTTTGAATCATTCAAAAAGATAAAAACCTGTGGCAGTGATGGAAGCAATAAAGTTGCTGCACTGAATGAAACACATTCTCATCATGCTGATTCATCACTGAAATTGAGAAATGAAGTTAGCTCAGTTAAAATTCCAGTGGCTAAAGAGTTAGTGGATAGAGTAATTCCTTCAGATGCATCTGAGATTGAAAGGACTCAGAAAAATAACTATTCTGAATACAAGGTAAAGGGGGCACCCCTGTCTGATAAAGATTCATCCACCAATGTGACCTCTGGACATCTCTTTCCAACACTTCCTGAAAACCCTGAACAAAAGGAACAGATCTTCTCTGGTATTTCGAACAGAGCAGCCAAAAAATGCCAGTCTGCTAATGTATCAGGCAATGATCATCAGGTAGCAGTATCAGGAAATGAACATGATTTGATTGATGACACTTGTCAAAGTGCCAACAAAAAATTTACTGAAATTTCAAAGTCCTGCGATAAAGTGATGAAGGTCATTGAAAACAAGCCTGTGGAGTTGACTTCACATCCATGCGGTCAGGAGTTCATTCCAGAAAGTAATGATATTGTGATGATGAACATTGGAAAAGAGTCTGTGGATTTGACTTCAGATCCATGCAGTCGGGAGGTCATTCCAGAAAGAAAGGATAAAGCTGGGACCATCATTGAAAATGTTATTGTGGACTTGACTTCAGATCAATGCAGTCAGGAGGTCATTGAAAAAGAGGAATCATTAGATCTGTCCACCAAGGTTGGAATAGAAATTTCTTCAGGTCCAGCTGAGTTTGAAAGGACTGAGGAATGTAACAAATCTGAAGCCAAGGTCAATAGGGTGCCGTCTGATAAAGATTCATGCACTGAGACCTCTGGGCATCCCTTCCCAACACATTCTGAAAATCCTGAAGAGAATGGAGAGACCACCTCAAACAAAGCAGCAAAAGAATGCCGGAATGCTGATGGATCAGAATTATTATCAGGCAAGGATCAGCTGGTACTATCACAAAAGGAACCTGATTTAATGTATGCTAGTTCCCCAAGAGACAACAAGAAATTGACTGGGATTTCAGAGTCTATTCATAATCCCCTTACTTTTGAATGCCAAAATTATGATGTGATCATCAATGAGGAGTCTGTTGAGGTGACTTGTCAGAAGGTCATTCCACAGAGAAAGGATGTGATCATCAATGAGAAGGAGTCTGTTGAGGTGACTTGTCAGAAGGTCATTCCACAGAGAAAGGATACCATCATCATTGAAAACAAGTCTGTTGAGGTGACTTTAGAACCATGCAGCCAGGAGGTTATTCCAGAAAGAAAGGATACAATCGTCATTGGAGAAGAGTCTGTTGATTTGACTTTAGATCCAAGCTGTCAAGAGTTCATTCCAGAAACAAAGGGGGAATCATTAAAGGTTTTCAACAGGGTACACAGACAGCGTGCTAATACAAATATAAGAGCAGTTCCTTCAGATGCATCTGACCTTGAAAGGACTGAGAAAAGAAATTATTCTGAAGTTAAGGCCAGAAAGGTATCAACTTCTGATAAAGTTCCACACACCAAGCAGACCTCTTGTCATCTCAACCCAATACGTCATGTACATGTTGAAGAAGATAAAACAAACATCCCTAAGATTTCAAACAGAGGAATAAACAAACTTAAGACTACTGATGGATCAGTGGCTGCATCCCAAAAGGAACAAGTTTCTATCGATGACAGCTGCCAACAAGCGAAAAAGAAATTTACTGGAATTTCAAAGTCTTTTGAAGCTTCGGTTACTTCTGGATCCCAATATTATTCAGCCCTATCATCAAAGATCTCTAACAAGTTGCAGAGTCAGAGTAATATTGCAAGAAAAAATGACAGCGCTCTTTCAGTGGTAAAACCTGAACCCATTGAAGATGCAGGTGCAACCTTTCCAAGTTTCACAATCTCCTGTTTGGCAACTGACCGTCAATCTTTTCTGGAGTTGCCAAAGAGTTTGCCATTACCCTTGAACACCATGAAGGGAAGGTCTAAACTGGATAGGAGGATAGTTTATCTTCAAGGTCCTGATAAGAGATTGTGGCCGGTCCTCTACATTCAGAAATTTTTCTTCAGAACTTTGGCAAATGGTTGGGAAGCTTTCAGCATGGCAAATAACATTGAACAAGGAGATCGATGTACTATTGAGGTCACAAATCAAACTGAAGGCATATGTGGTGTGCACATAGTTAAGATGAGAGATGATATGGAATTCTATGAAGAGTATGAACACAGTCCTCTCATTGACCTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

1046

Amino Acids

116.89

Weight (kDa)

5.79

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 84 - 173 8.2e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2753
Acc36I ACCTGC 1 cut(s) 2753
AccB1I GGYRCC 2 cut(s) 1093, 1696
AciI CCGC 1 cut(s) 1398
AclWI GGATC 8 cut(s) 102, 1469, 1831, 1854, 2216, 2539, 2637, 2650
AcoI YGGCCR 1 cut(s) 2924
AcvI CACGTG 1 cut(s) 359
AfaI GTAC 6 cut(s) 607, 613, 1857, 2286, 2457, 3022
AfeI AGCGCT 1 cut(s) 2725
AfiI CCNNNNNNNGG 1 cut(s) 148
AflII CTTAAG 1 cut(s) 2516
AflIII ACRYGT 2 cut(s) 786, 2458
AhdI GACNNNNNGTC 2 cut(s) 787, 2430
AjnI CCWGG 2 cut(s) 401, 2154
AleI CACNNNNGTG 1 cut(s) 1124
Alw21I GWGCWC 2 cut(s) 148, 3066
Alw26I GTCTC 4 cut(s) 539, 1718, 1772, 1896
Alw44I GTGCAC 1 cut(s) 3062
AlwI GGATC 8 cut(s) 102, 1469, 1831, 1854, 2216, 2539, 2637, 2650
AlwNI CAGNNNCTG 1 cut(s) 2540
Aor51HI AGCGCT 1 cut(s) 2725
AoxI GGCC 4 cut(s) 5, 405, 2379, 2924
ApaI GGGCCC 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 3062
ApeKI GCWGC 6 cut(s) 917, 1208, 1796, 2151, 2540, 2577
Asp700I GAANNNNTTC 2 cut(s) 2365, 2948
AspLEI GCGC 2 cut(s) 765, 2726
AspS9I GGNCC 8 cut(s) 5, 6, 405, 647, 1521, 1637, 2906, 2929
AsuHPI GGTGA 3 cut(s) 2014, 2080, 2146
AsuII TTCGAA 2 cut(s) 171, 1199
AvaII GGWCC 5 cut(s) 647, 1521, 1637, 2906, 2929
BaeGI GKGCMC 3 cut(s) 9, 1096, 3066
BaeI ACNNNNGTAYC 2 cut(s) 2172, 2205
BamHI GGATCC 1 cut(s) 2642
BanI GGYRCC 2 cut(s) 1093, 1696
BanII GRGCYC 2 cut(s) 9, 841
BbrPI CACGTG 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 463
Bbv12I GWGCWC 2 cut(s) 148, 3066
BbvI GCAGC 6 cut(s) 904, 1220, 1808, 2163, 2527, 2564
BccI CCATC 9 cut(s) 151, 414, 578, 793, 893, 1532, 1814, 2114, 2522
BceAI ACGGC 1 cut(s) 1684
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciT130I CCWGG 2 cut(s) 403, 2156
BciVI GTATCC 2 cut(s) 2095, 2173
BclI TGATCA 3 cut(s) 1246, 1977, 2040
BcoDI GTCTC 4 cut(s) 539, 1718, 1772, 1896
BfaI CTAG 4 cut(s) 387, 437, 858, 1890
BfoI RGCGCY 1 cut(s) 2727
BfrI CTTAAG 1 cut(s) 2516
BfuAI ACCTGC 1 cut(s) 2753
BfuI GTATCC 2 cut(s) 2095, 2173
BglII AGATCT 3 cut(s) 1182, 1600, 2673
BisI GCNGC 6 cut(s) 918, 1209, 1797, 2152, 2541, 2578
BlsI GCNGC 6 cut(s) 919, 1210, 1798, 2153, 2542, 2579
Bme1390I CCNGG 2 cut(s) 403, 2156
Bme18I GGWCC 5 cut(s) 647, 1521, 1637, 2906, 2929
BmeRI GACNNNNNGTC 2 cut(s) 787, 2430
BmgT120I GGNCC 8 cut(s) 5, 6, 405, 647, 1521, 1637, 2906, 2929
BmiI GGNNCC 9 cut(s) 6, 7, 232, 400, 1095, 1522, 1698, 1872, 2644
BmrFI CCNGG 2 cut(s) 403, 2156
BmrI ACTGGG 1 cut(s) 1929
BmsI GCATC 9 cut(s) 625, 643, 1024, 1046, 1744, 2320, 2342, 2552, 2749
BmuI ACTGGG 1 cut(s) 1929
BoxI GACNNNNGTC 1 cut(s) 1296
BpiI GAAGAC 1 cut(s) 463
BplI GAGNNNNNCTC 2 cut(s) 1769, 1801
BpmI CTGGAG 1 cut(s) 2843
Bpu14I TTCGAA 2 cut(s) 171, 1199
Bsa29I ATCGAT 2 cut(s) 2568, 3016
BsaAI YACGTR 1 cut(s) 359
BsaI GGTCTC 3 cut(s) 539, 1718, 1772
BsaJI CCNNGG 3 cut(s) 402, 1611, 1683
BsaWI WCCGGW 2 cut(s) 16, 366
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse118I RCCGGY 1 cut(s) 2926
Bse1I ACTGG 6 cut(s) 153, 995, 1222, 1924, 2608, 2883
Bse3DI GCAATG 1 cut(s) 1249
BseBI CCWGG 2 cut(s) 403, 2156
BseCI ATCGAT 2 cut(s) 2568, 3016
BseDI CCNNGG 3 cut(s) 402, 1611, 1683
BseGI GGATG 7 cut(s) 425, 1115, 1390, 1735, 2041, 2481, 2543
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMI GCAATG 1 cut(s) 1249
BseMII CTCAG 8 cut(s) 206, 996, 1032, 1070, 1635, 1650, 1713, 2343
BseNI ACTGG 6 cut(s) 153, 995, 1222, 1924, 2608, 2883
BseRI GAGGAG 1 cut(s) 2003
BseSI GKGCMC 3 cut(s) 9, 1096, 3066
BseXI GCAGC 6 cut(s) 904, 1220, 1808, 2163, 2527, 2564
BseYI CCCAGC 1 cut(s) 1517
BsgI GTGCAG 2 cut(s) 201, 903
BshFI GGCC 4 cut(s) 7, 407, 2381, 2926
BshNI GGYRCC 2 cut(s) 1093, 1696
BshVI ATCGAT 2 cut(s) 2568, 3016
BsiHKAI GWGCWC 2 cut(s) 148, 3066
BsiSI CCGG 4 cut(s) 17, 367, 1810, 2927
BslFI GGGAC 1 cut(s) 1534
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 4 cut(s) 539, 1718, 1772, 1896
BsmFI GGGAC 1 cut(s) 1534
BsmI GAATGC 4 cut(s) 430, 1811, 1819, 1964
BsnI GGCC 4 cut(s) 7, 407, 2381, 2926
Bso31I GGTCTC 3 cut(s) 539, 1718, 1772
Bsp119I TTCGAA 2 cut(s) 171, 1199
Bsp120I GGGCCC 1 cut(s) 5
Bsp1286I GDGCHC 5 cut(s) 9, 148, 841, 1096, 3066
Bsp1407I TGTACA 1 cut(s) 2455
BspACI CCGC 1 cut(s) 1398
BspANI GGCC 4 cut(s) 7, 407, 2381, 2926
BspCNI CTCAG 8 cut(s) 205, 995, 1033, 1069, 1636, 1651, 1714, 2344
BspDI ATCGAT 2 cut(s) 2568, 3016
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 9 cut(s) 6, 7, 232, 400, 1095, 1522, 1698, 1872, 2644
BspMI ACCTGC 1 cut(s) 2753
BspPI GGATC 8 cut(s) 102, 1469, 1831, 1854, 2216, 2539, 2637, 2650
BspT104I TTCGAA 2 cut(s) 171, 1199
BspT107I GGYRCC 2 cut(s) 1093, 1696
BspTI CTTAAG 1 cut(s) 2516
BspTNI GGTCTC 3 cut(s) 539, 1718, 1772
BsrDI GCAATG 1 cut(s) 1249
BsrFI RCCGGY 1 cut(s) 2926
BsrGI TGTACA 1 cut(s) 2455
BsrI ACTGG 6 cut(s) 153, 995, 1222, 1924, 2608, 2883
BssAI RCCGGY 1 cut(s) 2926
BssECI CCNNGG 3 cut(s) 402, 1611, 1683
BssT1I CCWWGG 2 cut(s) 1611, 1683
Bst2UI CCWGG 2 cut(s) 403, 2156
Bst4CI ACNGT 5 cut(s) 276, 502, 616, 2810, 3116
Bst6I CTCTTC 4 cut(s) 147, 1761, 2193, 3096
BstAFI CTTAAG 1 cut(s) 2516
BstAPI GCANNNNNTGC 1 cut(s) 776
BstAUI TGTACA 1 cut(s) 2455
BstBAI YACGTR 1 cut(s) 359
BstBI TTCGAA 2 cut(s) 171, 1199
BstC8I GCNNGC 2 cut(s) 761, 2298
BstDEI CTNAG 9 cut(s) 192, 982, 1041, 1056, 1644, 1659, 1722, 2352, 2487
BstF5I GGATG 7 cut(s) 425, 1115, 1390, 1735, 2041, 2481, 2543
BstH2I RGCGCY 1 cut(s) 2727
BstHHI GCGC 2 cut(s) 765, 2726
BstMAI GTCTC 4 cut(s) 539, 1718, 1772, 1896
BstMWI GCNNNNNNNGC 1 cut(s) 776
BstNI CCWGG 2 cut(s) 403, 2156
BstNSI RCATGY 2 cut(s) 790, 2462
BstPAI GACNNNNGTC 1 cut(s) 1296
BstSCI CCNGG 2 cut(s) 401, 2154
BstSLI GKGCMC 3 cut(s) 9, 1096, 3066
BstV1I GCAGC 6 cut(s) 904, 1220, 1808, 2163, 2527, 2564
BstV2I GAAGAC 1 cut(s) 463
BstX2I RGATCY 7 cut(s) 107, 1182, 1474, 1600, 2221, 2642, 2673
BstXI CCANNNNNNTGG 1 cut(s) 602
BstYI RGATCY 7 cut(s) 107, 1182, 1474, 1600, 2221, 2642, 2673
Bsu15I ATCGAT 2 cut(s) 2568, 3016
BsuI GTATCC 2 cut(s) 2095, 2173
BsuRI GGCC 4 cut(s) 7, 407, 2381, 2926
BsuTUI ATCGAT 2 cut(s) 2568, 3016
BtsCI GGATG 7 cut(s) 425, 1115, 1390, 1735, 2041, 2481, 2543
BtsI GCAGTG 1 cut(s) 901
BveI ACCTGC 1 cut(s) 2753
Cac8I GCNNGC 2 cut(s) 761, 2298
CaiI CAGNNNCTG 1 cut(s) 2540
CciI TCATGA 1 cut(s) 163
CfoI GCGC 2 cut(s) 765, 2726
Cfr10I RCCGGY 1 cut(s) 2926
Cfr13I GGNCC 8 cut(s) 5, 6, 405, 647, 1521, 1637, 2906, 2929
ClaI ATCGAT 2 cut(s) 2568, 3016
Csp6I GTAC 6 cut(s) 606, 612, 1856, 2285, 2456, 3021
CspCI CAANNNNNGTGG 2 cut(s) 1597, 1632
CviQI GTAC 6 cut(s) 606, 612, 1856, 2285, 2456, 3021
DdeI CTNAG 9 cut(s) 192, 982, 1041, 1056, 1644, 1659, 1722, 2352, 2487
DriI GACNNNNNGTC 2 cut(s) 787, 2430
EaeI YGGCCR 1 cut(s) 2924
Eam1104I CTCTTC 4 cut(s) 147, 1761, 2193, 3096
Eam1105I GACNNNNNGTC 2 cut(s) 787, 2430
EarI CTCTTC 4 cut(s) 147, 1761, 2193, 3096
Eco130I CCWWGG 2 cut(s) 1611, 1683
Eco24I GRGCYC 2 cut(s) 9, 841
Eco31I GGTCTC 3 cut(s) 539, 1718, 1772
Eco47I GGWCC 5 cut(s) 647, 1521, 1637, 2906, 2929
Eco47III AGCGCT 1 cut(s) 2725
Eco72I CACGTG 1 cut(s) 359
EcoO109I RGGNCCY 2 cut(s) 5, 2906
EcoRI GAATTC 1 cut(s) 3092
EcoRII CCWGG 2 cut(s) 401, 2154
EcoT14I CCWWGG 2 cut(s) 1611, 1683
EcoT22I ATGCAT 2 cut(s) 1039, 2335
EcoT38I GRGCYC 2 cut(s) 9, 841
ErhI CCWWGG 2 cut(s) 1611, 1683
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 1 cut(s) 1534
FauNDI CATATG 1 cut(s) 3053
FbaI TGATCA 3 cut(s) 1246, 1977, 2040
Fnu4HI GCNGC 6 cut(s) 918, 1209, 1797, 2152, 2541, 2578
FokI GGATG 7 cut(s) 432, 1102, 1377, 1722, 2048, 2468, 2530
FriOI GRGCYC 2 cut(s) 9, 841
Fsp4HI GCNGC 6 cut(s) 918, 1209, 1797, 2152, 2541, 2578
FspBI CTAG 4 cut(s) 387, 437, 858, 1890
GlaI GCGC 2 cut(s) 764, 2725
GluI GCNGC 6 cut(s) 918, 1209, 1797, 2152, 2541, 2578
GsaI CCCAGC 1 cut(s) 1521
GsuI CTGGAG 1 cut(s) 2843
HaeII RGCGCY 1 cut(s) 2727
HaeIII GGCC 4 cut(s) 7, 407, 2381, 2926
HapII CCGG 4 cut(s) 17, 367, 1810, 2927
HhaI GCGC 2 cut(s) 765, 2726
Hin6I GCGC 2 cut(s) 763, 2724
HinP1I GCGC 2 cut(s) 763, 2724
HincII GTYRAC 2 cut(s) 84, 1383
HindII GTYRAC 2 cut(s) 84, 1383
HindIII AAGCTT 3 cut(s) 174, 2625, 2979
HpaII CCGG 4 cut(s) 17, 367, 1810, 2927
HphI GGTGA 3 cut(s) 2014, 2080, 2146
Hpy166II GTNNAC 9 cut(s) 84, 362, 491, 577, 1383, 1546, 1608, 2287, 3064
Hpy8I GTNNAC 9 cut(s) 84, 362, 491, 577, 1383, 1546, 1608, 2287, 3064
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 5 cut(s) 276, 502, 616, 2810, 3116
HpyCH4IV ACGT 3 cut(s) 358, 480, 2449
HpyF10VI GCNNNNNNNGC 1 cut(s) 776
HpyF3I CTNAG 9 cut(s) 192, 982, 1041, 1056, 1644, 1659, 1722, 2352, 2487
HpySE526I ACGT 3 cut(s) 358, 480, 2449
HspAI GCGC 2 cut(s) 763, 2724
Ksp22I TGATCA 3 cut(s) 1246, 1977, 2040
Lsp1109I GCAGC 6 cut(s) 904, 1220, 1808, 2163, 2527, 2564
LweI GCATC 9 cut(s) 625, 643, 1024, 1046, 1744, 2320, 2342, 2552, 2749
MaeI CTAG 4 cut(s) 387, 437, 858, 1890
MaeII ACGT 3 cut(s) 358, 480, 2449
MaeIII GTNAC 7 cut(s) 1126, 1667, 2002, 2068, 2134, 2632, 3031
MflI RGATCY 7 cut(s) 107, 1182, 1474, 1600, 2221, 2642, 2673
MhlI GDGCHC 5 cut(s) 9, 148, 841, 1096, 3066
MlyI GAGTC 8 cut(s) 357, 1048, 1463, 1940, 2000, 2066, 2210, 2701
MmeI TCCRAC 2 cut(s) 1172, 1597
Mph1103I ATGCAT 2 cut(s) 1039, 2335
MroXI GAANNNNTTC 2 cut(s) 2365, 2948
MseI TTAA 9 cut(s) 102, 393, 504, 987, 1880, 2267, 2376, 2517, 3072
MslI CAYNNNNRTG 5 cut(s) 582, 735, 1124, 1393, 2457
MspA1I CMGCKG 3 cut(s) 1643, 1852, 2577
MspCI CTTAAG 1 cut(s) 2516
MspI CCGG 4 cut(s) 17, 367, 1810, 2927
MspR9I CCNGG 2 cut(s) 403, 2156
Mva1269I GAATGC 4 cut(s) 430, 1811, 1819, 1964
MvaI CCWGG 2 cut(s) 403, 2156
MwoI GCNNNNNNNGC 1 cut(s) 776
NdeI CATATG 1 cut(s) 3053
NlaIV GGNNCC 9 cut(s) 6, 7, 232, 400, 1095, 1522, 1698, 1872, 2644
NmuCI GTSAC 5 cut(s) 1126, 2002, 2068, 2134, 3031
NsiI ATGCAT 2 cut(s) 1039, 2335
NspI RCATGY 2 cut(s) 790, 2462
NspV TTCGAA 2 cut(s) 171, 1199
OliI CACNNNNGTG 1 cut(s) 1124
PagI TCATGA 1 cut(s) 163
PaqCI CACCTGC 1 cut(s) 2753
PciI ACATGT 2 cut(s) 786, 2458
PctI GAATGC 4 cut(s) 430, 1811, 1819, 1964
PdmI GAANNNNTTC 2 cut(s) 2365, 2948
PkrI GCNGC 6 cut(s) 919, 1210, 1798, 2153, 2542, 2579
PleI GAGTC 8 cut(s) 357, 1048, 1462, 1939, 1999, 2065, 2209, 2700
PmaCI CACGTG 1 cut(s) 359
PmlI CACGTG 1 cut(s) 359
PpsI GAGTC 8 cut(s) 357, 1048, 1462, 1939, 1999, 2065, 2209, 2700
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 2906
PscI ACATGT 2 cut(s) 786, 2458
PshAI GACNNNNGTC 1 cut(s) 1296
Psp5II RGGWCCY 1 cut(s) 2906
Psp6I CCWGG 2 cut(s) 401, 2154
PspCI CACGTG 1 cut(s) 359
PspFI CCCAGC 1 cut(s) 1517
PspGI CCWGG 2 cut(s) 401, 2154
PspN4I GGNNCC 9 cut(s) 6, 7, 232, 400, 1095, 1522, 1698, 1872, 2644
PspOMI GGGCCC 1 cut(s) 5
PspPI GGNCC 8 cut(s) 5, 6, 405, 647, 1521, 1637, 2906, 2929
PspPPI RGGWCCY 1 cut(s) 2906
PstNI CAGNNNCTG 1 cut(s) 2540
PsuI RGATCY 7 cut(s) 107, 1182, 1474, 1600, 2221, 2642, 2673
PvuII CAGCTG 3 cut(s) 1643, 1852, 2577
RsaI GTAC 6 cut(s) 607, 613, 1857, 2286, 2457, 3022
RsaNI GTAC 6 cut(s) 606, 612, 1856, 2285, 2456, 3021
RseI CAYNNNNRTG 5 cut(s) 582, 735, 1124, 1393, 2457
SaqAI TTAA 9 cut(s) 102, 393, 504, 987, 1880, 2267, 2376, 2517, 3072
SatI GCNGC 6 cut(s) 918, 1209, 1797, 2152, 2541, 2578
Sau96I GGNCC 8 cut(s) 5, 6, 405, 647, 1521, 1637, 2906, 2929
SchI GAGTC 8 cut(s) 357, 1048, 1463, 1940, 2000, 2066, 2210, 2701
ScrFI CCNGG 2 cut(s) 403, 2156
SduI GDGCHC 5 cut(s) 9, 148, 841, 1096, 3066
SfaNI GCATC 9 cut(s) 625, 643, 1024, 1046, 1744, 2320, 2342, 2552, 2749
SfuI TTCGAA 2 cut(s) 171, 1199
SinI GGWCC 5 cut(s) 647, 1521, 1637, 2906, 2929
SmiMI CAYNNNNRTG 5 cut(s) 582, 735, 1124, 1393, 2457
SmlI CTYRAG 1 cut(s) 2516
SmoI CTYRAG 1 cut(s) 2516
SsiI CCGC 1 cut(s) 1398
SspI AATATT 2 cut(s) 2651, 2704
SspMI CTAG 4 cut(s) 387, 437, 858, 1890
StyD4I CCNGG 2 cut(s) 401, 2154
StyI CCWWGG 2 cut(s) 1611, 1683
TaaI ACNGT 5 cut(s) 276, 502, 616, 2810, 3116
TaiI ACGT 3 cut(s) 361, 483, 2452
TaqI TCGA 5 cut(s) 171, 240, 1199, 2568, 3016
TatI WGTACW 3 cut(s) 611, 2455, 3020
Tru1I TTAA 9 cut(s) 102, 393, 504, 987, 1880, 2267, 2376, 2517, 3072
Tru9I TTAA 9 cut(s) 102, 393, 504, 987, 1880, 2267, 2376, 2517, 3072
TseFI GTSAC 5 cut(s) 1126, 2002, 2068, 2134, 3031
TseI GCWGC 6 cut(s) 917, 1208, 1796, 2151, 2540, 2577
Tsp45I GTSAC 5 cut(s) 1126, 2002, 2068, 2134, 3031
TspGWI ACGGA 1 cut(s) 616
Vha464I CTTAAG 1 cut(s) 2516
VneI GTGCAC 1 cut(s) 3062
VpaK11BI GGWCC 5 cut(s) 647, 1521, 1637, 2906, 2929
XceI RCATGY 2 cut(s) 790, 2462
XmnI GAANNNNTTC 2 cut(s) 2365, 2948
XspI CTAG 4 cut(s) 387, 437, 858, 1890
Zsp2I ATGCAT 2 cut(s) 1039, 2335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.