Rorug02G0410100

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002691
Physical Location & Seq
Forward (+)
52575509 .. 52580983
5475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0410100.1

Sequence Viewer

Length: 1101 bp
ATGACTCGGCGCTGTTCCCACTGCAGCAACAACGGCCACAACTCCCGCACGTGCCCCTCGCGTGGCGGAGGTGGCGGCTCCTCCTCCTGCTCTTCTGGAGCGGGCGGCGGCGTGAAGCTCTTCGGCGTTAGGCTGACGGATGGCTCCTTCATTAAGAAGAGCGCTAGCATGGGCAATTTGTCCACTATGGCCTCGCACTACCAATCCTCGTCTCCCAACCCGGACTCTCCTCCCGGCTACGACCCGCCGCTCCACGACCCGGTTCATGTCCGCGAAGGTTACTTGTCGGACGACCCGGCCCATGCCTCCTCCTCCACCAATCGCCGTGGAGATCGTAAAAAAGGAGTTCCATGGACAGAAGAGGAGCATCGAATGTTCTTAATTGGTCTTCAAAAATTGGGGAAAGGAGACTGGCGCGGGATAGCGCGAAATTATGTAACGACTAGGACTCCTACTCAGGTGGCAAGCCATGCCCAGAAGTATTTTATCCGGCAGAGTAATTCTACCAGAAGAAAGAGACGCTCCAGTCTCTTTGACATGGCCCCTGACATGGCCATGGATCCACCTCACATGCCAGAAGAACAAGCTTTGCTGCCTTCTTGTCAGGAGGAAAGAGAATCTGAAGATTCAAAACCTTCATTAAATCTTTCCCTTGGTTCAGAATGCAGCAGACCTATGGAAAGCACACAAGAAGAAAAAGTGATTATAGAACCCGATAATCAGCCGGTAATGGGATTGAATGGGTACCTGAATGGTTACCCGCCAGTTTTTCCCGGCTTCATCCCAGCGTATTTGCCTTATCCTTATCCTGTATGGCCAAGTGCAGGTCCTCTGATAATAGAAAACGGAGGCGAGGCTTCTCATTATCCGCAGGTCTTAAAGCCGGTTCCTATGCTTCCCAAGGAACCTGTCAACGTTGATGCTCTAGTAGGTATGTCTCAGCTCAGTCTAGTAGAGGCCGAGAGTCTCCAAAGCAATCCTTCCCCTCTCTCTCTGACATCATTAGGAGAGCCGTCAAGGCAGTCGGCATTTCACCCAAATGCTCCGGCCAGTGAACCAGACTTAAGCAAGGGCAAGACCAGTGCCATCCAAGCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

366

Amino Acids

39.61

Weight (kDa)

6.83

Isoelectric Point (pI)

71.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 116 - 162 5.5e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 525
Acc36I ACCTGC 2 cut(s) 815, 862
Acc65I GGTACC 1 cut(s) 744
AccB1I GGYRCC 1 cut(s) 744
AccBSI CCGCTC 2 cut(s) 101, 250
AccII CGCG 4 cut(s) 61, 273, 417, 427
AclI AACGTT 1 cut(s) 915
AclWI GGATC 2 cut(s) 554, 567
AcoI YGGCCR 4 cut(s) 34, 552, 815, 1047
AcuI CTGAAG 1 cut(s) 642
AcvI CACGTG 1 cut(s) 51
AfaI GTAC 1 cut(s) 746
AfeI AGCGCT 1 cut(s) 163
AfiI CCNNNNNNNGG 5 cut(s) 62, 259, 550, 731, 824
AflII CTTAAG 1 cut(s) 1063
AgsI TTSAA 3 cut(s) 392, 630, 739
AluBI AGCT 3 cut(s) 118, 587, 943
AluI AGCT 3 cut(s) 118, 587, 943
Alw26I GTCTC 6 cut(s) 216, 402, 511, 533, 942, 971
AlwI GGATC 2 cut(s) 554, 567
Aor51HI AGCGCT 1 cut(s) 163
AoxI GGCC 8 cut(s) 34, 189, 297, 540, 552, 815, 957, 1047
ApeKI GCWGC 3 cut(s) 24, 592, 666
Asp700I GAANNNNTTC 1 cut(s) 119
Asp718I GGTACC 1 cut(s) 744
AspLEI GCGC 4 cut(s) 12, 164, 417, 427
AspS9I GGNCC 3 cut(s) 298, 541, 827
AsuC2I CCSGG 5 cut(s) 221, 234, 260, 296, 774
AsuHPI GGTGA 1 cut(s) 1025
AsuNHI GCTAGC 1 cut(s) 164
AvaII GGWCC 1 cut(s) 827
BaeGI GKGCMC 1 cut(s) 56
BalI TGGCCA 2 cut(s) 554, 817
BamHI GGATCC 1 cut(s) 559
BanI GGYRCC 1 cut(s) 744
BbrPI CACGTG 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 380
BbvI GCAGC 3 cut(s) 36, 579, 678
BccI CCATC 2 cut(s) 134, 1094
BceAI ACGGC 3 cut(s) 49, 309, 997
BcnI CCSGG 5 cut(s) 221, 234, 260, 296, 774
BcoDI GTCTC 6 cut(s) 216, 402, 511, 533, 942, 971
BfaI CTAG 4 cut(s) 165, 444, 926, 950
BfmI CTRYAG 1 cut(s) 22
BfoI RGCGCY 2 cut(s) 13, 165
BfrI CTTAAG 1 cut(s) 1063
BfuAI ACCTGC 2 cut(s) 815, 862
BglI GCCNNNNNGGC 1 cut(s) 1018
BisI GCNGC 7 cut(s) 25, 76, 106, 109, 248, 593, 667
BlsI GCNGC 7 cut(s) 26, 77, 107, 110, 249, 594, 668
Bme1390I CCNGG 5 cut(s) 221, 234, 260, 296, 774
Bme18I GGWCC 1 cut(s) 827
BmgT120I GGNCC 3 cut(s) 298, 541, 827
BmiI GGNNCC 7 cut(s) 79, 145, 543, 561, 746, 888, 906
BmrFI CCNGG 5 cut(s) 221, 234, 260, 296, 774
BmsI GCATC 2 cut(s) 376, 910
BmtI GCTAGC 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 380
BpmI CTGGAG 2 cut(s) 117, 508
BpuMI CCSGG 5 cut(s) 221, 234, 260, 296, 774
BsaAI YACGTR 1 cut(s) 51
BsaJI CCNNGG 5 cut(s) 325, 350, 555, 652, 900
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 259, 550, 731, 824
Bse118I RCCGGY 2 cut(s) 724, 883
Bse1I ACTGG 5 cut(s) 416, 525, 764, 1050, 1080
BseDI CCNNGG 5 cut(s) 325, 350, 555, 652, 900
BseGI GGATG 3 cut(s) 145, 780, 1086
BseLI CCNNNNNNNGG 5 cut(s) 62, 259, 550, 731, 824
BseMII CTCAG 3 cut(s) 470, 953, 958
BseNI ACTGG 5 cut(s) 416, 525, 764, 1050, 1080
BseRI GAGGAG 6 cut(s) 70, 73, 219, 298, 301, 377
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 3 cut(s) 36, 579, 678
BseYI CCCAGC 1 cut(s) 784
BsgI GTGCAG 1 cut(s) 843
Bsh1236I CGCG 4 cut(s) 61, 273, 417, 427
BshFI GGCC 8 cut(s) 36, 191, 299, 542, 554, 817, 959, 1049
BshNI GGYRCC 1 cut(s) 744
BsiSI CCGG 9 cut(s) 221, 234, 260, 296, 490, 725, 774, 884, 1046
BslI CCNNNNNNNGG 5 cut(s) 62, 259, 550, 731, 824
BsmAI GTCTC 6 cut(s) 216, 402, 511, 533, 942, 971
BsmBI CGTCTC 2 cut(s) 216, 511
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 8 cut(s) 36, 191, 299, 542, 554, 817, 959, 1049
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 2 cut(s) 331, 559
Bsp19I CCATGG 2 cut(s) 350, 555
BspANI GGCC 8 cut(s) 36, 191, 299, 542, 554, 817, 959, 1049
BspCNI CTCAG 3 cut(s) 469, 952, 957
BspFNI CGCG 4 cut(s) 61, 273, 417, 427
BspLI GGNNCC 7 cut(s) 79, 145, 543, 561, 746, 888, 906
BspMAI CTGCAG 1 cut(s) 26
BspMI ACCTGC 2 cut(s) 815, 862
BspOI GCTAGC 1 cut(s) 168
BspPI GGATC 2 cut(s) 554, 567
BspQI GCTCTTC 3 cut(s) 97, 125, 152
BspT107I GGYRCC 1 cut(s) 744
BspTI CTTAAG 1 cut(s) 1063
BsrBI CCGCTC 2 cut(s) 101, 250
BsrFI RCCGGY 2 cut(s) 724, 883
BsrI ACTGG 5 cut(s) 416, 525, 764, 1050, 1080
BssAI RCCGGY 2 cut(s) 724, 883
BssECI CCNNGG 5 cut(s) 325, 350, 555, 652, 900
BssMI GATC 2 cut(s) 331, 559
BssT1I CCWWGG 4 cut(s) 350, 555, 652, 900
Bst6I CTCTTC 4 cut(s) 97, 125, 152, 354
BstAFI CTTAAG 1 cut(s) 1063
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 3 cut(s) 103, 166, 466
BstDEI CTNAG 3 cut(s) 456, 939, 944
BstDSI CCRYGG 3 cut(s) 325, 350, 555
BstEII GGTNACC 1 cut(s) 755
BstF5I GGATG 3 cut(s) 145, 780, 1086
BstFNI CGCG 4 cut(s) 61, 273, 417, 427
BstH2I RGCGCY 2 cut(s) 13, 165
BstHHI GCGC 4 cut(s) 12, 164, 417, 427
BstKTI GATC 2 cut(s) 334, 562
BstMAI GTCTC 6 cut(s) 216, 402, 511, 533, 942, 971
BstMBI GATC 2 cut(s) 331, 559
BstMWI GCNNNNNNNGC 5 cut(s) 33, 72, 470, 1018, 1091
BstNSI RCATGY 1 cut(s) 574
BstPI GGTNACC 1 cut(s) 755
BstSCI CCNGG 5 cut(s) 219, 232, 258, 294, 772
BstSFI CTRYAG 1 cut(s) 22
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 4 cut(s) 61, 273, 417, 427
BstV1I GCAGC 3 cut(s) 36, 579, 678
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 8 cut(s) 36, 191, 299, 542, 554, 817, 959, 1049
BtgI CCRYGG 3 cut(s) 325, 350, 555
BtsCI GGATG 3 cut(s) 145, 780, 1086
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 3 cut(s) 19, 1057, 1087
BveI ACCTGC 2 cut(s) 815, 862
Cac8I GCNNGC 3 cut(s) 103, 166, 466
CfoI GCGC 4 cut(s) 12, 164, 417, 427
Cfr10I RCCGGY 2 cut(s) 724, 883
Cfr13I GGNCC 3 cut(s) 298, 541, 827
CseI GACGC 1 cut(s) 528
Csp6I GTAC 1 cut(s) 745
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 9 cut(s) 169, 266, 302, 351, 470, 538, 550, 556, 571
CviQI GTAC 1 cut(s) 745
DdeI CTNAG 3 cut(s) 456, 939, 944
DpnI GATC 2 cut(s) 333, 561
DpnII GATC 2 cut(s) 331, 559
DrdI GACNNNNNNGTC 1 cut(s) 525
DseDI GACNNNNNNGTC 1 cut(s) 525
EaeI YGGCCR 4 cut(s) 34, 552, 815, 1047
Eam1104I CTCTTC 4 cut(s) 97, 125, 152, 354
EarI CTCTTC 4 cut(s) 97, 125, 152, 354
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 4 cut(s) 350, 555, 652, 900
Eco47I GGWCC 1 cut(s) 827
Eco47III AGCGCT 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 642
Eco72I CACGTG 1 cut(s) 51
Eco91I GGTNACC 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 827
EcoO65I GGTNACC 1 cut(s) 755
EcoT14I CCWWGG 4 cut(s) 350, 555, 652, 900
ErhI CCWWGG 4 cut(s) 350, 555, 652, 900
Esp3I CGTCTC 2 cut(s) 216, 511
FaeI CATG 9 cut(s) 172, 269, 305, 354, 473, 541, 553, 559, 574
FalI AAGNNNNNCTT 2 cut(s) 964, 996
FatI CATG 9 cut(s) 168, 265, 301, 350, 469, 537, 549, 555, 570
FauI CCCGC 5 cut(s) 53, 94, 252, 410, 768
Fnu4HI GCNGC 7 cut(s) 25, 76, 106, 109, 248, 593, 667
FokI GGATG 3 cut(s) 152, 767, 1073
Fsp4HI GCNGC 7 cut(s) 25, 76, 106, 109, 248, 593, 667
FspBI CTAG 4 cut(s) 165, 444, 926, 950
GlaI GCGC 4 cut(s) 11, 163, 416, 426
GluI GCNGC 7 cut(s) 25, 76, 106, 109, 248, 593, 667
GsaI CCCAGC 1 cut(s) 788
GsuI CTGGAG 2 cut(s) 117, 508
HaeII RGCGCY 2 cut(s) 13, 165
HaeIII GGCC 8 cut(s) 36, 191, 299, 542, 554, 817, 959, 1049
HapII CCGG 9 cut(s) 221, 234, 260, 296, 490, 725, 774, 884, 1046
HgaI GACGC 1 cut(s) 528
HhaI GCGC 4 cut(s) 12, 164, 417, 427
Hin1II CATG 9 cut(s) 172, 269, 305, 354, 473, 541, 553, 559, 574
Hin6I GCGC 4 cut(s) 10, 162, 415, 425
HinP1I GCGC 4 cut(s) 10, 162, 415, 425
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 6 cut(s) 4, 224, 448, 617, 626, 964
HpaII CCGG 9 cut(s) 221, 234, 260, 296, 490, 725, 774, 884, 1046
HphI GGTGA 1 cut(s) 1025
Hpy166II GTNNAC 3 cut(s) 183, 913, 1055
Hpy188I TCNGA 6 cut(s) 289, 622, 661, 834, 996, 1100
Hpy188III TCNNGA 2 cut(s) 96, 605
Hpy8I GTNNAC 3 cut(s) 183, 913, 1055
HpyAV CCTTC 5 cut(s) 157, 269, 606, 645, 990
HpyCH4IV ACGT 2 cut(s) 50, 915
HpyCH4V TGCA 3 cut(s) 24, 666, 824
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 72, 470, 1018, 1091
HpyF3I CTNAG 3 cut(s) 456, 939, 944
HpySE526I ACGT 2 cut(s) 50, 915
Hsp92II CATG 9 cut(s) 172, 269, 305, 354, 473, 541, 553, 559, 574
HspAI GCGC 4 cut(s) 10, 162, 415, 425
KpnI GGTACC 1 cut(s) 748
Kzo9I GATC 2 cut(s) 331, 559
LguI GCTCTTC 3 cut(s) 97, 125, 152
LmnI GCTCC 7 cut(s) 83, 98, 149, 255, 364, 527, 1048
Lsp1109I GCAGC 3 cut(s) 36, 579, 678
LweI GCATC 2 cut(s) 376, 910
MaeI CTAG 4 cut(s) 165, 444, 926, 950
MaeII ACGT 2 cut(s) 50, 915
MaeIII GTNAC 3 cut(s) 278, 436, 755
MalI GATC 2 cut(s) 333, 561
MbiI CCGCTC 2 cut(s) 101, 250
MboI GATC 2 cut(s) 331, 559
MboII GAAGA 9 cut(s) 84, 112, 169, 371, 380, 522, 590, 635, 704
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 56
MlsI TGGCCA 2 cut(s) 554, 817
MluCI AATT 5 cut(s) 175, 381, 395, 430, 499
MluNI TGGCCA 2 cut(s) 554, 817
MlyI GAGTC 3 cut(s) 218, 442, 973
MmeI TCCRAC 1 cut(s) 267
Mox20I TGGCCA 2 cut(s) 554, 817
MroXI GAANNNNTTC 1 cut(s) 119
MscI TGGCCA 2 cut(s) 554, 817
MseI TTAA 5 cut(s) 153, 380, 641, 878, 1064
MslI CAYNNNNRTG 2 cut(s) 554, 1038
Msp20I TGGCCA 2 cut(s) 554, 817
MspCI CTTAAG 1 cut(s) 1063
MspI CCGG 9 cut(s) 221, 234, 260, 296, 490, 725, 774, 884, 1046
MspR9I CCNGG 5 cut(s) 221, 234, 260, 296, 774
Mva1269I GAATGC 1 cut(s) 668
MvnI CGCG 4 cut(s) 61, 273, 417, 427
MwoI GCNNNNNNNGC 5 cut(s) 33, 72, 470, 1018, 1091
NciI CCSGG 5 cut(s) 221, 234, 260, 296, 774
NcoI CCATGG 2 cut(s) 350, 555
NdeII GATC 2 cut(s) 331, 559
NheI GCTAGC 1 cut(s) 164
NlaIII CATG 9 cut(s) 172, 269, 305, 354, 473, 541, 553, 559, 574
NlaIV GGNNCC 7 cut(s) 79, 145, 543, 561, 746, 888, 906
NmeAIII GCCGAG 1 cut(s) 985
NspI RCATGY 1 cut(s) 574
PciSI GCTCTTC 3 cut(s) 97, 125, 152
PctI GAATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 617, 626
PkrI GCNGC 7 cut(s) 26, 77, 107, 110, 249, 594, 668
PleI GAGTC 3 cut(s) 218, 442, 972
PmaCI CACGTG 1 cut(s) 51
PmlI CACGTG 1 cut(s) 51
PpsI GAGTC 3 cut(s) 218, 442, 972
Ppu21I YACGTR 1 cut(s) 51
PpuMI RGGWCCY 1 cut(s) 827
Psp1406I AACGTT 1 cut(s) 915
Psp5II RGGWCCY 1 cut(s) 827
PspCI CACGTG 1 cut(s) 51
PspEI GGTNACC 1 cut(s) 755
PspFI CCCAGC 1 cut(s) 784
PspN4I GGNNCC 7 cut(s) 79, 145, 543, 561, 746, 888, 906
PspPI GGNCC 3 cut(s) 298, 541, 827
PspPPI RGGWCCY 1 cut(s) 827
PstI CTGCAG 1 cut(s) 26
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 1 cut(s) 746
RsaNI GTAC 1 cut(s) 745
RseI CAYNNNNRTG 2 cut(s) 554, 1038
SapI GCTCTTC 3 cut(s) 97, 125, 152
SaqAI TTAA 5 cut(s) 153, 380, 641, 878, 1064
SatI GCNGC 7 cut(s) 25, 76, 106, 109, 248, 593, 667
Sau3AI GATC 2 cut(s) 331, 559
Sau96I GGNCC 3 cut(s) 298, 541, 827
SchI GAGTC 3 cut(s) 218, 442, 973
ScrFI CCNGG 5 cut(s) 221, 234, 260, 296, 774
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 2 cut(s) 376, 910
SfcI CTRYAG 1 cut(s) 22
SinI GGWCC 1 cut(s) 827
SmiMI CAYNNNNRTG 2 cut(s) 554, 1038
SmlI CTYRAG 1 cut(s) 1063
SmoI CTYRAG 1 cut(s) 1063
Sse9I AATT 5 cut(s) 175, 381, 395, 430, 499
SspMI CTAG 4 cut(s) 165, 444, 926, 950
StyD4I CCNGG 5 cut(s) 219, 232, 258, 294, 772
StyI CCWWGG 4 cut(s) 350, 555, 652, 900
TaiI ACGT 2 cut(s) 53, 918
TaqI TCGA 1 cut(s) 370
TasI AATT 5 cut(s) 175, 381, 395, 430, 499
TauI GCSGC 4 cut(s) 78, 108, 111, 250
TfiI GAWTC 2 cut(s) 617, 626
Tru1I TTAA 5 cut(s) 153, 380, 641, 878, 1064
Tru9I TTAA 5 cut(s) 153, 380, 641, 878, 1064
TscAI CASTG 3 cut(s) 26, 1057, 1087
TseI GCWGC 3 cut(s) 24, 592, 666
TspDTI ATGAA 4 cut(s) 139, 254, 627, 769
TspGWI ACGGA 2 cut(s) 152, 861
TspRI CASTG 3 cut(s) 26, 1057, 1087
Vha464I CTTAAG 1 cut(s) 1063
VpaK11BI GGWCC 1 cut(s) 827
XceI RCATGY 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 4 cut(s) 165, 444, 926, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.