Rh2AG471000

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002691
Physical Location & Seq
Reverse (-)
69066111 .. 69070233
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG471000.1

Sequence Viewer

Length: 1080 bp
ATGTATCTTGCAGGTGTAACAATATTCATCCAAACAGGAACAGAGATGGGTCACGAATTCAAAGCTTGTGTGGAATGCACTGAGAAGTGTTTGCTGGTTCACGGGAACAAGAGTGATGCTTCACCTGTCATAACTTCCTTTGTCACAGTCATGTCTGGAAACGAGTTTTCTAAATTTATGGAATTGCCTCCAAATGTTGCTCGTGCTGTGTCTTTGGCTGATCAATCGACTGTCATTGAAGATTCACAGGGGCTGCGATGGAACGTTGCAATCTCAAGTATGGATGGCTCCTTTGCTTTTCATCATGGATGGAATGCTTTTTCATTAGATCATGACCTACAACTAGGAAATATTCTGGTCTTCACTTACATTAGGGGTTCACATTTTGCTGTTAAAATCTATGATAACTCTGGGTGTGAACTAAGTTCTTTTGAGACCGATCAGAAGAAAAGAAAAAGGGATGACAGAGATTCTATTTTCAAAGGTGGTTGGTGCTATGCAACTGATAAAAGTTCAATGAGCAAAGACGGTTCAGGCACCTGTGGTTGCTCAGATGCAGAGATAAGTAAACGGTTGGATGAAGTGAATGGCAAGGGGAAAGCTCCAATCACCACACAGTTTGCTTCAAATTGTGATGATAGAAAGGAAGCTGGCTCCGTTAACAGCCCAGTGGCTAAAGATGTAGGGAGTACAGTAATTCCTTCAGATGGATCTGAGTTGGAAAGGACACTGAAAAATAGCGATCATGAAGTCAAGGTAAAAAAGGAAGAACCTTCTGAAATACATTCATGCACCGAGACCTCTGGAAAACTCTTATCAACACTTACTGTAATTCCGGAAGAAAATGAAGAGATCATCTCTGCTGTGTCAACCAGAGCTACAAAAAAATGCCGCACTGGCGATGGATCAGGAGGTGAGGAAGCGATTTCAAGAGATCAGAGGCTCAAACCCGATCATCCCACTTTTGTTGTCATGATGCAAGAACCCTCCTACCTTACAACTGCCTTGGTAAGCTTCTTTTATTTGTTTATTCTTTTTCAACAAACCCACTCCTTACAGAAGATAGAGATTTGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

359

Amino Acids

39.52

Weight (kDa)

5.37

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 47 - 136 1.1e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2
Acc36I ACCTGC 1 cut(s) 2
AccB1I GGYRCC 1 cut(s) 536
AccIII TCCGGA 1 cut(s) 835
AciI CCGC 1 cut(s) 892
AclI AACGTT 1 cut(s) 264
AclWI GGATC 2 cut(s) 718, 913
AcsI RAATTY 2 cut(s) 56, 173
AcuI CTGAAG 1 cut(s) 687
AfaI GTAC 1 cut(s) 691
AfiI CCNNNNNNNGG 1 cut(s) 707
AgsI TTSAA 7 cut(s) 61, 239, 481, 516, 627, 930, 1040
AluBI AGCT 5 cut(s) 65, 602, 650, 878, 1014
AluI AGCT 5 cut(s) 65, 602, 650, 878, 1014
Alw26I GTCTC 2 cut(s) 428, 791
AlwI GGATC 2 cut(s) 718, 913
AlwNI CAGNNNCTG 1 cut(s) 253
Aor13HI TCCGGA 1 cut(s) 835
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 2 cut(s) 56, 173
AsuHPI GGTGA 3 cut(s) 114, 601, 926
BanI GGYRCC 1 cut(s) 536
BauI CACGAG 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 352
BbvI GCAGC 1 cut(s) 240
BccI CCATC 6 cut(s) 40, 252, 278, 303, 701, 896
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 2 cut(s) 428, 791
BfaI CTAG 1 cut(s) 344
BfuAI ACCTGC 1 cut(s) 2
BglI GCCNNNNNGGC 1 cut(s) 897
BisI GCNGC 2 cut(s) 254, 892
BlsI GCNGC 2 cut(s) 255, 893
BmiI GGNNCC 3 cut(s) 289, 538, 655
BmrI ACTGGG 1 cut(s) 662
BmsI GCATC 3 cut(s) 106, 544, 966
BmuI ACTGGG 1 cut(s) 662
BpiI GAAGAC 1 cut(s) 352
BplI GAGNNNNNCTC 2 cut(s) 842, 874
BpuEI CTTGAG 1 cut(s) 259
BsaI GGTCTC 2 cut(s) 428, 791
BsaJI CCNNGG 1 cut(s) 1005
BsaWI WCCGGW 1 cut(s) 835
Bsc4I CCNNNNNNNGG 1 cut(s) 707
Bse1I ACTGG 2 cut(s) 668, 901
BseAI TCCGGA 1 cut(s) 835
BseDI CCNNGG 1 cut(s) 1005
BseGI GGATG 6 cut(s) 27, 289, 314, 466, 583, 955
BseLI CCNNNNNNNGG 1 cut(s) 707
BseMII CTCAG 3 cut(s) 72, 564, 705
BseNI ACTGG 2 cut(s) 668, 901
BseXI GCAGC 1 cut(s) 240
BshNI GGYRCC 1 cut(s) 536
BsiSI CCGG 1 cut(s) 836
BslI CCNNNNNNNGG 1 cut(s) 707
BsmAI GTCTC 2 cut(s) 428, 791
BsmI GAATGC 2 cut(s) 80, 319
Bso31I GGTCTC 2 cut(s) 428, 791
Bsp13I TCCGGA 1 cut(s) 835
Bsp143I GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
BspACI CCGC 1 cut(s) 892
BspCNI CTCAG 3 cut(s) 73, 563, 706
BspEI TCCGGA 1 cut(s) 835
BspHI TCATGA 3 cut(s) 331, 745, 972
BspLI GGNNCC 3 cut(s) 289, 538, 655
BspMI ACCTGC 1 cut(s) 2
BspPI GGATC 2 cut(s) 718, 913
BspT107I GGYRCC 1 cut(s) 536
BspTNI GGTCTC 2 cut(s) 428, 791
BsrI ACTGG 2 cut(s) 668, 901
BssECI CCNNGG 1 cut(s) 1005
BssMI GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 1005
Bst2BI CACGAG 1 cut(s) 201
Bst4CI ACNGT 7 cut(s) 148, 232, 530, 573, 618, 694, 829
Bst6I CTCTTC 1 cut(s) 843
BstC8I GCNNGC 1 cut(s) 652
BstDEI CTNAG 4 cut(s) 81, 422, 550, 714
BstF5I GGATG 6 cut(s) 27, 289, 314, 466, 583, 955
BstKTI GATC 9 cut(s) 223, 331, 442, 713, 745, 855, 908, 937, 955
BstMAI GTCTC 2 cut(s) 428, 791
BstMBI GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
BstMWI GCNNNNNNNGC 1 cut(s) 897
BstV1I GCAGC 1 cut(s) 240
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
BtgZI GCGATG 2 cut(s) 271, 915
BtsCI GGATG 6 cut(s) 27, 289, 314, 466, 583, 955
BtsIMutI CAGTG 4 cut(s) 78, 675, 728, 894
BveI ACCTGC 1 cut(s) 2
Cac8I GCNNGC 1 cut(s) 652
CaiI CAGNNNCTG 1 cut(s) 253
CciI TCATGA 3 cut(s) 331, 745, 972
Csp6I GTAC 1 cut(s) 690
CspCI CAANNNNNGTGG 4 cut(s) 601, 636, 949, 984
CviAII CATG 6 cut(s) 151, 305, 332, 746, 789, 973
CviQI GTAC 1 cut(s) 690
DdeI CTNAG 4 cut(s) 81, 422, 550, 714
DpnI GATC 9 cut(s) 222, 330, 441, 712, 744, 854, 907, 936, 954
DpnII GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
Eco130I CCWWGG 1 cut(s) 1005
Eco31I GGTCTC 2 cut(s) 428, 791
Eco57I CTGAAG 1 cut(s) 687
EcoRI GAATTC 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 1005
ErhI CCWWGG 1 cut(s) 1005
FaeI CATG 6 cut(s) 154, 308, 335, 749, 792, 976
FatI CATG 6 cut(s) 150, 304, 331, 745, 788, 972
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 254, 892
FokI GGATG 6 cut(s) 14, 296, 321, 473, 590, 942
Fsp4HI GCNGC 2 cut(s) 254, 892
FspBI CTAG 1 cut(s) 344
GluI GCNGC 2 cut(s) 254, 892
HapII CCGG 1 cut(s) 836
Hin1II CATG 6 cut(s) 154, 308, 335, 749, 792, 976
HincII GTYRAC 2 cut(s) 661, 870
HindII GTYRAC 2 cut(s) 661, 870
HindIII AAGCTT 2 cut(s) 63, 1012
HinfI GANTC 2 cut(s) 242, 470
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 1 cut(s) 836
HphI GGTGA 3 cut(s) 114, 601, 926
Hpy166II GTNNAC 6 cut(s) 100, 380, 419, 569, 661, 870
Hpy188I TCNGA 6 cut(s) 444, 553, 706, 715, 778, 939
Hpy188III TCNNGA 9 cut(s) 53, 156, 332, 746, 804, 836, 909, 930, 973
Hpy8I GTNNAC 6 cut(s) 100, 380, 419, 569, 661, 870
HpyAV CCTTC 2 cut(s) 711, 783
HpyCH4III ACNGT 7 cut(s) 148, 232, 530, 573, 618, 694, 829
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 7 cut(s) 11, 78, 269, 500, 557, 792, 979
HpyF10VI GCNNNNNNNGC 1 cut(s) 897
HpyF3I CTNAG 4 cut(s) 81, 422, 550, 714
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 6 cut(s) 154, 308, 335, 749, 792, 976
Kpn2I TCCGGA 1 cut(s) 835
Ksp22I TGATCA 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
LmnI GCTCC 3 cut(s) 293, 607, 659
Lsp1109I GCAGC 1 cut(s) 240
LweI GCATC 3 cut(s) 106, 544, 966
MaeI CTAG 1 cut(s) 344
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 3 cut(s) 16, 50, 142
MalI GATC 9 cut(s) 222, 330, 441, 712, 744, 854, 907, 936, 954
MboI GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
MboII GAAGA 7 cut(s) 251, 352, 457, 779, 851, 860, 1072
MflI RGATCY 1 cut(s) 710
MluCI AATT 6 cut(s) 56, 173, 182, 628, 696, 831
MmeI TCCRAC 2 cut(s) 555, 699
MnlI CCTC 6 cut(s) 198, 811, 905, 910, 933, 997
MroI TCCGGA 1 cut(s) 835
MseI TTAA 2 cut(s) 393, 660
MslI CAYNNNNRTG 1 cut(s) 149
MspI CCGG 1 cut(s) 836
Mva1269I GAATGC 2 cut(s) 80, 319
MwoI GCNNNNNNNGC 1 cut(s) 897
NdeII GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
NlaIII CATG 6 cut(s) 154, 308, 335, 749, 792, 976
NlaIV GGNNCC 3 cut(s) 289, 538, 655
NmuCI GTSAC 2 cut(s) 50, 142
PagI TCATGA 3 cut(s) 331, 745, 972
PaqCI CACCTGC 1 cut(s) 2
PctI GAATGC 2 cut(s) 80, 319
PfeI GAWTC 2 cut(s) 242, 470
PkrI GCNGC 2 cut(s) 255, 893
Psp1406I AACGTT 1 cut(s) 264
PspN4I GGNNCC 3 cut(s) 289, 538, 655
PstNI CAGNNNCTG 1 cut(s) 253
PsuI RGATCY 1 cut(s) 710
RsaI GTAC 1 cut(s) 691
RsaNI GTAC 1 cut(s) 690
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 2 cut(s) 393, 660
SatI GCNGC 2 cut(s) 254, 892
Sau3AI GATC 9 cut(s) 220, 328, 439, 710, 742, 852, 905, 934, 952
SfaNI GCATC 3 cut(s) 106, 544, 966
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 6 cut(s) 56, 173, 182, 628, 696, 831
SsiI CCGC 1 cut(s) 892
SspI AATATT 2 cut(s) 24, 352
SspMI CTAG 1 cut(s) 344
StyI CCWWGG 1 cut(s) 1005
TaaI ACNGT 7 cut(s) 148, 232, 530, 573, 618, 694, 829
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 227
TaqII GACCGA 1 cut(s) 452
TasI AATT 6 cut(s) 56, 173, 182, 628, 696, 831
TatI WGTACW 1 cut(s) 689
TauI GCSGC 1 cut(s) 894
TfiI GAWTC 2 cut(s) 242, 470
Tru1I TTAA 2 cut(s) 393, 660
Tru9I TTAA 2 cut(s) 393, 660
TscAI CASTG 4 cut(s) 85, 675, 735, 901
TseFI GTSAC 2 cut(s) 50, 142
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 2 cut(s) 50, 142
TspDTI ATGAA 7 cut(s) 16, 290, 312, 594, 762, 777, 861
TspGWI ACGGA 1 cut(s) 646
TspRI CASTG 4 cut(s) 85, 675, 735, 901
XapI RAATTY 2 cut(s) 56, 173
XspI CTAG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.