RchiOBHm_Chr2g0149281

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002691
Physical Location & Seq
Reverse (-)
67138680 .. 67141331
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51869

Sequence Viewer

Length: 594 bp
ATGGGTCACGAATTCAAAGCTTGTGTGGAATGCACTGAGAAGTGTTTGCTGGTTCATGGGAACAAGAGTGATGCTTCACCTGTCATAACTTCCTTTGTCACAGTCATGTCTGGAAACGAGTTTTCTAAATTTATGGAATTGCCTCCAAATGTTGCTCGTGCTGTGTCTTTGGCTGATCAATCGACTGTCATTGAAGATTCACAGGGGCTGCGATGGAACATTGCAATCTCAAGTATGGATGGCTCCTTTGCTTTTCATCATGGATGGAATGCTTTTTCATTAGATCATGACCTACAACTAGGAAATATTCTGGTCTTCACTTACATTAGGGGTTCACATTTTGCTGTTAAAATCTATGATAACTCTGGGTGTGAACCATGTTCTTTTGAGACCGATCAGAAGAAAAGAAAAAGGGATGACAGAGATTCTATTTTCAAAGGTGGTTGGTGCTATGCAACTGATAAAAGTTCAATGAGCAAAGACGGTTCAGGCACCTGTGGTTGCTCAGATGCAGAGATAAGTAAACGGTTGGATGAAGTGAATGGCAAGGGGAAAGCTCCAATCACCACACAGTTAATTTGCTTCAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

197

Amino Acids

21.7

Weight (kDa)

6.06

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 32 - 121 1.1e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 491
AcsI RAATTY 2 cut(s) 11, 128
AgsI TTSAA 5 cut(s) 16, 194, 436, 471, 586
AluBI AGCT 2 cut(s) 20, 557
AluI AGCT 2 cut(s) 20, 557
Alw26I GTCTC 1 cut(s) 383
AlwNI CAGNNNCTG 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 208
ApoI RAATTY 2 cut(s) 11, 128
AsuHPI GGTGA 2 cut(s) 69, 556
BanI GGYRCC 1 cut(s) 491
BauI CACGAG 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 195
BccI CCATC 3 cut(s) 207, 233, 258
BclI TGATCA 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 1 cut(s) 299
BisI GCNGC 1 cut(s) 209
BlsI GCNGC 1 cut(s) 210
BmiI GGNNCC 2 cut(s) 244, 493
BmsI GCATC 2 cut(s) 61, 499
BpiI GAAGAC 1 cut(s) 307
BpuEI CTTGAG 1 cut(s) 214
BsaI GGTCTC 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 219
BseGI GGATG 4 cut(s) 244, 269, 421, 538
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 2 cut(s) 27, 519
BseXI GCAGC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 491
BsmAI GTCTC 1 cut(s) 383
BsmI GAATGC 2 cut(s) 35, 274
Bso31I GGTCTC 1 cut(s) 383
Bsp143I GATC 3 cut(s) 175, 283, 394
BspCNI CTCAG 2 cut(s) 28, 518
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 244, 493
BspT107I GGYRCC 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 219
BssMI GATC 3 cut(s) 175, 283, 394
BssSI CACGAG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 156
Bst4CI ACNGT 5 cut(s) 103, 187, 485, 528, 573
BstDEI CTNAG 2 cut(s) 36, 505
BstF5I GGATG 4 cut(s) 244, 269, 421, 538
BstKTI GATC 3 cut(s) 178, 286, 397
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 3 cut(s) 175, 283, 394
BstV1I GCAGC 1 cut(s) 195
BstV2I GAAGAC 1 cut(s) 307
BtgZI GCGATG 1 cut(s) 226
BtsCI GGATG 4 cut(s) 244, 269, 421, 538
BtsIMutI CAGTG 1 cut(s) 33
CaiI CAGNNNCTG 1 cut(s) 208
CciI TCATGA 1 cut(s) 286
CviAII CATG 5 cut(s) 56, 106, 260, 287, 378
CviJI RGCY 5 cut(s) 20, 173, 208, 243, 557
CviKI_1 RGCY 5 cut(s) 20, 173, 208, 243, 557
DdeI CTNAG 2 cut(s) 36, 505
DpnI GATC 3 cut(s) 177, 285, 396
DpnII GATC 3 cut(s) 175, 283, 394
Eco31I GGTCTC 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 11
FaeI CATG 5 cut(s) 59, 109, 263, 290, 381
FatI CATG 5 cut(s) 55, 105, 259, 286, 377
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 1 cut(s) 209
FokI GGATG 4 cut(s) 251, 276, 428, 545
Fsp4HI GCNGC 1 cut(s) 209
FspBI CTAG 1 cut(s) 299
GluI GCNGC 1 cut(s) 209
Hin1II CATG 5 cut(s) 59, 109, 263, 290, 381
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 2 cut(s) 197, 425
HphI GGTGA 2 cut(s) 69, 556
Hpy166II GTNNAC 3 cut(s) 335, 374, 524
Hpy188I TCNGA 2 cut(s) 399, 508
Hpy188III TCNNGA 3 cut(s) 8, 111, 287
Hpy8I GTNNAC 3 cut(s) 335, 374, 524
HpyCH4III ACNGT 5 cut(s) 103, 187, 485, 528, 573
HpyCH4V TGCA 4 cut(s) 33, 224, 455, 512
HpyF3I CTNAG 2 cut(s) 36, 505
Hsp92II CATG 5 cut(s) 59, 109, 263, 290, 381
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 3 cut(s) 175, 283, 394
LmnI GCTCC 2 cut(s) 248, 562
LpnPI CCDG 8 cut(s) 35, 93, 96, 188, 296, 351, 474, 508
Lsp1109I GCAGC 1 cut(s) 195
LweI GCATC 2 cut(s) 61, 499
MaeI CTAG 1 cut(s) 299
MaeIII GTNAC 2 cut(s) 5, 97
MalI GATC 3 cut(s) 177, 285, 396
MboI GATC 3 cut(s) 175, 283, 394
MboII GAAGA 3 cut(s) 206, 307, 412
MluCI AATT 5 cut(s) 11, 128, 137, 576, 587
MmeI TCCRAC 1 cut(s) 510
MnlI CCTC 1 cut(s) 153
MseI TTAA 2 cut(s) 348, 575
MslI CAYNNNNRTG 1 cut(s) 104
Mva1269I GAATGC 2 cut(s) 35, 274
NdeII GATC 3 cut(s) 175, 283, 394
NlaIII CATG 5 cut(s) 59, 109, 263, 290, 381
NlaIV GGNNCC 2 cut(s) 244, 493
NmuCI GTSAC 2 cut(s) 5, 97
PagI TCATGA 1 cut(s) 286
PctI GAATGC 2 cut(s) 35, 274
PfeI GAWTC 2 cut(s) 197, 425
PkrI GCNGC 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 244, 493
PstNI CAGNNNCTG 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 2 cut(s) 348, 575
SatI GCNGC 1 cut(s) 209
Sau3AI GATC 3 cut(s) 175, 283, 394
SetI ASST 6 cut(s) 22, 82, 294, 442, 497, 559
SfaNI GCATC 2 cut(s) 61, 499
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 5 cut(s) 11, 128, 137, 576, 587
SspI AATATT 1 cut(s) 307
SspMI CTAG 1 cut(s) 299
TaaI ACNGT 5 cut(s) 103, 187, 485, 528, 573
TaqI TCGA 1 cut(s) 182
TaqII GACCGA 1 cut(s) 407
TasI AATT 5 cut(s) 11, 128, 137, 576, 587
TfiI GAWTC 2 cut(s) 197, 425
Tru1I TTAA 2 cut(s) 348, 575
Tru9I TTAA 2 cut(s) 348, 575
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 2 cut(s) 5, 97
TseI GCWGC 1 cut(s) 208
Tsp45I GTSAC 2 cut(s) 5, 97
TspDTI ATGAA 4 cut(s) 44, 245, 267, 549
TspRI CASTG 1 cut(s) 40
XapI RAATTY 2 cut(s) 11, 128
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.