Rroxscaffold_3G00263910

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002691
Physical Location & Seq
Forward (+)
57712368 .. 57719380
7013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263910.1

Sequence Viewer

Length: 2949 bp
ATGGTTCAAGATTTCGAAGCTTGTGCAGATTGCACTCAGAAGTGTTTGCTGATTCATAGGAATAAGAAAAATGGTTCCCATATCGAAACCTCTTTTTTCAAAATCATGTTTGGAAAACAGTTTTCTAAAGTTATGGAATTGCCTCCAAAAGTTGCTCGTATTTTGTTATTGTCTGATCGGAGAACTTTTCTTGAAGATTCACGTGGACTGCGGTGGAAAGTTGCAATATCTAGTGTTAATGGTTCCTGGGCCTTTCAACAAGGATGGAATGCTTTTGCACTAGACCATGACCTACAAGTAGGAGATTTTCTGGTCTTCAATTATGTTGTGGGTTCACATTTTACTGTTACGATATATGACAACTCTGGGTGTGAAGTGAATCTTTTTGAGACCATACATCAGAACAACAGAAATAGGGTTCACACAGATGGTCAGTGCCACACCATTGGTAAAAGTACAGTGAGCAAATCCGTTTTAGATACCTTTGCTGGTCCAGATGCAGAAATAAGTAATTGCTTGAATGAAGTGCATGGTATGAAGAAACCTCTAATTATCACAGAAAATGCTTCAGATCACGATGATGATTATGAAAGGTCAAAGTGCAAGCGCAAAGCAGAATTTGCCGACGACATGTCATGTATGATGGAAAGAGAATATGGACACCAACAAGGAGAGAACAGAGCCCGTATTCTTGATTTGTCTAGTTTTGAATCATTCAAAAAGATAAAAACCTGTGGCAGTGATGGAAGCAATAAAGTTGCTGCAGTGAATGAAACACATTCTCATCATGCTGATTCATCACTGAAATTGAGAAATGAAGTTAGCTCAGTTAAAATTCCAGTGGCCAAAGAGTTAGTGGATAGAGTAATTCCTTCAGATGCATCTGAGATTGAAAGGACTCAGAAAAATAACTATTCTGAATACAAGGTAAAGGGGGCACCCCTGTCTGATAAAGATTCATCCACCAATGTGACCTCTGGACATCTCTTTCCAACACTTCCTGAAAATCCTGAACAAAAGGAAGAGATCTTCTCTGGTTTTTCGAACAGAGCAGCCAAAAAATGCCAGTCTGCTAATGTATCAGGCAATGATCATCAGGTAGCAGTACCAGGAAATGAACATGATTTGATTGATGACACTTGTCAAAGTGCCAACAAAAAAATTACTGAAATTTCAAAGTCCTGCAGTATTCCCATTACTTTCCTCTCCCAAAATGATAAAGTGATGAAGGTCATTGAAAACAAGCCTGTGGAGTTGACTTCACATCCATGCGGTCAGGAGGTCATTCCAGAAAATATTGTGATGATGAACATTGGAAAAGAGTCTGTGGCTTTGACTTCAGATCCATGCAGTCGGGAGGTCATTCCAGAAAGAAAGGATAAAGCTGTGACCATCATTGAAAATGTTATTGTGGACTTGACTTCAGATCAATGCAGTCAGGAGGTCATTGAAAAAGAGGAATCATTAGATCTTTCCACCAAGGTTGGAATAGAAATTCCTTCAGGTCCAGCTGAGTTTGAAAGGACTGAGGAATGTAACAAATCTGAAGCCAAGGTCAAAAGGGTGCCGTCTGATAAAGATTCATGCACTGAGACCTCTGGGCATCCCTTCCCAACACATTCTGAAAATCCTGAAGAAAATGGAGAGACCACCTCAAAGAAAGCAACAAAAGAATGCCAGAATGCTGATGGATCAGAATTATTATCAGGCAAGGATCAGGTGGTACTATCACAAAAGGAACCTGATTTAATGTATGCTAGTTCCTCAAGAGACAACAAGAAATTGACTGGGATTTCAGAGTCTATTCATAATCCCCTTTCTTTTGAATGCCAAAATTATGATGTGATCATCAATGAGAAGGAGTCTGCTGAGGTGCCTTGTCAGGAGGTCATTCCACAGAGAAAGGATACAATCGTCATTGGAGAAGAGTCTGTTGATTTGACTTTAGATCCAAGCTGTCAAGAGTTCATTCCAGAAACAAAGGGGGAATCATTAAAGGTTTTCAACAGGGTACACAGACAGCGTGCTAATACCAATATAAGAGCAGTTCCTTCAGATGCATCTGACCTTGAAAGGACTGAGAAAAGAAATTATTCTGAAGTTAAGGTCAGAAAGGTATCAACTTCTGATAAAGTTTCACACACCAAGCAGACCTCTTGTCATCTCAACCCAATACGTCATGTACATGTTGAAGAAGATAAAACAAACATCCCTAAGATTTCAAACAGAGGAATAAACAAACATAAGACTACTGATGGATCAGTGGCTGCATCCCAAAAGGAACAAGTTTCTATCGATGACAGCTGCCAACAAGCCAAAAAGAAATTTACTGGAATTTCAGAGTCTTTTGAAGCTCCGGTTACTTCTGGATCCCAATATTATTCAGCTAAAATGTCAAAGATCATGGAAAAGGAGTTGGTAAACTCCAGTTCAGATCCATGCAGTCAGGAGATTATTCCAGAAGTTGATGCCCTATCATCAAAGATCTCTAACAAGTTGCAGAGTCAGAGTAATTTTGCAAGAAAAAATGACAGCGCTCTTTCAGTGGTAAAACCTGAACCCATTGAAGATGCAGGTGCAACCTTTCCAAGTTTCACAATCTCCTGTTTGGCAACTGACCGTCAATCTTTTCTGGAGTTGCCAAAGAGTTTGCCATTACCCTTGAACACCATGAAGGGAAGGTCTAAACTGGATAGGAGGATAGTTTATCTTCAAGGTCCTGATAAGAGATTGTGGCCAGTCCTCTACATTCAGAAATTTTTCTTCAGAACTTTGGCAAATGGTTGGGAGGCTTTCAGCATGGCAAATAACATTGAACAAGGAGATCGATGTACTATTGAGGTAACAAATCAAACTGAAGGCATATGTAGTGTGCACATAGTTAAGATGAGAGATGATATGGAATTCTATGCAGAGAATGAGCACAGTCCTCTCATTGACCTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

982

Amino Acids

109.65

Weight (kDa)

5.64

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 42 - 121 2.4e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2564
Acc36I ACCTGC 1 cut(s) 2564
AccB1I GGYRCC 3 cut(s) 937, 1564, 1873
AciI CCGC 2 cut(s) 211, 1272
AclWI GGATC 8 cut(s) 1337, 1699, 1722, 1943, 2266, 2364, 2377, 2429
AcoI YGGCCR 2 cut(s) 843, 2735
AcsI RAATTY 8 cut(s) 617, 834, 1170, 1494, 2324, 2334, 2756, 2903
AcvI CACGTG 1 cut(s) 203
AfaI GTAC 6 cut(s) 457, 1107, 1725, 2013, 2184, 2833
AfeI AGCGCT 1 cut(s) 2536
AflIII ACRYGT 2 cut(s) 630, 2185
AhdI GACNNNNNGTC 2 cut(s) 631, 2157
AjnI CCWGG 2 cut(s) 245, 1108
AleI CACNNNNGTG 1 cut(s) 968
AluBI AGCT 8 cut(s) 20, 825, 1385, 1511, 1956, 2304, 2354, 2387
AluI AGCT 8 cut(s) 20, 825, 1385, 1511, 1956, 2304, 2354, 2387
Alw21I GWGCWC 2 cut(s) 2877, 2925
Alw26I GTCTC 4 cut(s) 383, 1586, 1640, 1764
Alw44I GTGCAC 1 cut(s) 2873
AlwI GGATC 8 cut(s) 1337, 1699, 1722, 1943, 2266, 2364, 2377, 2429
AlwNI CAGNNNCTG 1 cut(s) 2267
Aor51HI AGCGCT 1 cut(s) 2536
AoxI GGCC 3 cut(s) 249, 843, 2735
ApaLI GTGCAC 1 cut(s) 2873
ApeKI GCWGC 4 cut(s) 761, 1052, 2267, 2304
ApoI RAATTY 8 cut(s) 617, 834, 1170, 1494, 2324, 2334, 2756, 2903
Asp700I GAANNNNTTC 2 cut(s) 2092, 2759
AspLEI GCGC 2 cut(s) 609, 2537
AspS9I GGNCC 4 cut(s) 249, 491, 1505, 2717
AsuII TTCGAA 2 cut(s) 15, 1043
AvaII GGWCC 3 cut(s) 491, 1505, 2717
BaeGI GKGCMC 2 cut(s) 940, 2877
BaeI ACNNNNGTAYC 2 cut(s) 1899, 1932
BalI TGGCCA 2 cut(s) 845, 2737
BamHI GGATCC 1 cut(s) 2369
BanI GGYRCC 3 cut(s) 937, 1564, 1873
BanII GRGCYC 1 cut(s) 685
BbrPI CACGTG 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 307
Bbv12I GWGCWC 2 cut(s) 2877, 2925
BbvCI CCTCAGC 1 cut(s) 1869
BbvI GCAGC 4 cut(s) 748, 1064, 2254, 2291
BccI CCATC 7 cut(s) 258, 422, 637, 737, 1400, 1682, 2249
BceAI ACGGC 1 cut(s) 1552
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 247, 1110
BciVI GTATCC 1 cut(s) 1900
BclI TGATCA 2 cut(s) 1090, 1845
BcoDI GTCTC 4 cut(s) 383, 1586, 1640, 1764
BfaI CTAG 4 cut(s) 231, 281, 702, 1758
BfmI CTRYAG 2 cut(s) 762, 1183
BfoI RGCGCY 1 cut(s) 2538
BfuAI ACCTGC 1 cut(s) 2564
BfuI GTATCC 1 cut(s) 1900
BglII AGATCT 3 cut(s) 1026, 1468, 2484
BisI GCNGC 4 cut(s) 762, 1053, 2268, 2305
BlsI GCNGC 4 cut(s) 763, 1054, 2269, 2306
Bme1390I CCNGG 2 cut(s) 247, 1110
Bme18I GGWCC 3 cut(s) 491, 1505, 2717
BmeRI GACNNNNNGTC 2 cut(s) 631, 2157
BmgT120I GGNCC 4 cut(s) 249, 491, 1505, 2717
BmiI GGNNCC 7 cut(s) 76, 244, 939, 1566, 1740, 1875, 2371
BmrFI CCNGG 2 cut(s) 247, 1110
BmrI ACTGGG 1 cut(s) 1797
BmsI GCATC 9 cut(s) 487, 868, 890, 1612, 2047, 2069, 2279, 2458, 2560
BmuI ACTGGG 1 cut(s) 1797
BoxI GACNNNNGTC 1 cut(s) 1140
BpiI GAAGAC 1 cut(s) 307
BplI GAGNNNNNCTC 4 cut(s) 1016, 1048, 1637, 1669
BpmI CTGGAG 2 cut(s) 2410, 2654
Bpu10I CCTNAGC 1 cut(s) 1869
Bpu14I TTCGAA 2 cut(s) 15, 1043
BpuEI CTTGAG 1 cut(s) 1750
Bsa29I ATCGAT 2 cut(s) 2295, 2827
BsaAI YACGTR 1 cut(s) 203
BsaI GGTCTC 3 cut(s) 383, 1586, 1640
BsaJI CCNNGG 3 cut(s) 246, 1479, 1551
BsaWI WCCGGW 1 cut(s) 2356
Bse1I ACTGG 7 cut(s) 839, 1066, 1792, 2335, 2427, 2694, 2738
Bse3DI GCAATG 1 cut(s) 1093
BseBI CCWGG 2 cut(s) 247, 1110
BseCI ATCGAT 2 cut(s) 2295, 2827
BseDI CCNNGG 3 cut(s) 246, 1479, 1551
BseGI GGATG 6 cut(s) 269, 959, 1264, 1603, 2208, 2270
BseMI GCAATG 1 cut(s) 1093
BseMII CTCAG 9 cut(s) 50, 840, 876, 914, 1503, 1518, 1581, 1860, 2070
BseNI ACTGG 7 cut(s) 839, 1066, 1792, 2335, 2427, 2694, 2738
BseSI GKGCMC 2 cut(s) 940, 2877
BseXI GCAGC 4 cut(s) 748, 1064, 2254, 2291
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 3 cut(s) 251, 845, 2737
BshNI GGYRCC 3 cut(s) 937, 1564, 1873
BshVI ATCGAT 2 cut(s) 2295, 2827
BsiHKAI GWGCWC 2 cut(s) 2877, 2925
BsiSI CCGG 1 cut(s) 2357
BsmAI GTCTC 4 cut(s) 383, 1586, 1640, 1764
BsmI GAATGC 4 cut(s) 274, 1679, 1687, 1832
BsnI GGCC 3 cut(s) 251, 845, 2737
Bso31I GGTCTC 3 cut(s) 383, 1586, 1640
Bsp119I TTCGAA 2 cut(s) 15, 1043
Bsp1286I GDGCHC 4 cut(s) 685, 940, 2877, 2925
Bsp1407I TGTACA 1 cut(s) 2182
BspACI CCGC 2 cut(s) 211, 1272
BspANI GGCC 3 cut(s) 251, 845, 2737
BspCNI CTCAG 9 cut(s) 49, 839, 877, 913, 1504, 1519, 1582, 1861, 2071
BspDI ATCGAT 2 cut(s) 2295, 2827
BspLI GGNNCC 7 cut(s) 76, 244, 939, 1566, 1740, 1875, 2371
BspMAI CTGCAG 2 cut(s) 766, 1187
BspMI ACCTGC 1 cut(s) 2564
BspPI GGATC 8 cut(s) 1337, 1699, 1722, 1943, 2266, 2364, 2377, 2429
BspT104I TTCGAA 2 cut(s) 15, 1043
BspT107I GGYRCC 3 cut(s) 937, 1564, 1873
BspTNI GGTCTC 3 cut(s) 383, 1586, 1640
BsrDI GCAATG 1 cut(s) 1093
BsrGI TGTACA 1 cut(s) 2182
BsrI ACTGG 7 cut(s) 839, 1066, 1792, 2335, 2427, 2694, 2738
BssECI CCNNGG 3 cut(s) 246, 1479, 1551
BssT1I CCWWGG 2 cut(s) 1479, 1551
Bst2UI CCWGG 2 cut(s) 247, 1110
Bst4CI ACNGT 5 cut(s) 120, 346, 460, 2621, 2927
Bst6I CTCTTC 2 cut(s) 1017, 1920
BstAPI GCANNNNNTGC 1 cut(s) 620
BstAUI TGTACA 1 cut(s) 2182
BstBAI YACGTR 1 cut(s) 203
BstBI TTCGAA 2 cut(s) 15, 1043
BstC8I GCNNGC 2 cut(s) 605, 2025
BstF5I GGATG 6 cut(s) 269, 959, 1264, 1603, 2208, 2270
BstH2I RGCGCY 1 cut(s) 2538
BstHHI GCGC 2 cut(s) 609, 2537
BstMAI GTCTC 4 cut(s) 383, 1586, 1640, 1764
BstMWI GCNNNNNNNGC 1 cut(s) 620
BstNI CCWGG 2 cut(s) 247, 1110
BstNSI RCATGY 2 cut(s) 634, 2189
BstPAI GACNNNNGTC 1 cut(s) 1140
BstSCI CCNGG 2 cut(s) 245, 1108
BstSFI CTRYAG 2 cut(s) 762, 1183
BstSLI GKGCMC 2 cut(s) 940, 2877
BstV1I GCAGC 4 cut(s) 748, 1064, 2254, 2291
BstV2I GAAGAC 1 cut(s) 307
BstX2I RGATCY 7 cut(s) 1026, 1342, 1468, 1948, 2369, 2434, 2484
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 7 cut(s) 1026, 1342, 1468, 1948, 2369, 2434, 2484
Bsu15I ATCGAT 2 cut(s) 2295, 2827
BsuI GTATCC 1 cut(s) 1900
BsuRI GGCC 3 cut(s) 251, 845, 2737
BsuTUI ATCGAT 2 cut(s) 2295, 2827
BtsCI GGATG 6 cut(s) 269, 959, 1264, 1603, 2208, 2270
BtsI GCAGTG 2 cut(s) 745, 771
BtsIMutI CAGTG 9 cut(s) 440, 465, 745, 771, 800, 846, 1587, 2268, 2550
BveI ACCTGC 1 cut(s) 2564
Cac8I GCNNGC 2 cut(s) 605, 2025
CaiI CAGNNNCTG 1 cut(s) 2267
CfoI GCGC 2 cut(s) 609, 2537
Cfr13I GGNCC 4 cut(s) 249, 491, 1505, 2717
ClaI ATCGAT 2 cut(s) 2295, 2827
Csp6I GTAC 6 cut(s) 456, 1106, 1724, 2012, 2183, 2832
CspCI CAANNNNNGTGG 2 cut(s) 1465, 1500
CviQI GTAC 6 cut(s) 456, 1106, 1724, 2012, 2183, 2832
DriI GACNNNNNGTC 2 cut(s) 631, 2157
EaeI YGGCCR 2 cut(s) 843, 2735
Eam1104I CTCTTC 2 cut(s) 1017, 1920
Eam1105I GACNNNNNGTC 2 cut(s) 631, 2157
EarI CTCTTC 2 cut(s) 1017, 1920
Eco130I CCWWGG 2 cut(s) 1479, 1551
Eco24I GRGCYC 1 cut(s) 685
Eco31I GGTCTC 3 cut(s) 383, 1586, 1640
Eco47I GGWCC 3 cut(s) 491, 1505, 2717
Eco47III AGCGCT 1 cut(s) 2536
Eco72I CACGTG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 2717
EcoRI GAATTC 1 cut(s) 2903
EcoRII CCWGG 2 cut(s) 245, 1108
EcoT14I CCWWGG 2 cut(s) 1479, 1551
EcoT22I ATGCAT 2 cut(s) 883, 2062
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 2 cut(s) 1479, 1551
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FauNDI CATATG 1 cut(s) 2864
FbaI TGATCA 2 cut(s) 1090, 1845
Fnu4HI GCNGC 4 cut(s) 762, 1053, 2268, 2305
FokI GGATG 6 cut(s) 276, 946, 1251, 1590, 2195, 2257
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 4 cut(s) 762, 1053, 2268, 2305
FspBI CTAG 4 cut(s) 231, 281, 702, 1758
GlaI GCGC 2 cut(s) 608, 2536
GluI GCNGC 4 cut(s) 762, 1053, 2268, 2305
GsuI CTGGAG 2 cut(s) 2410, 2654
HaeII RGCGCY 1 cut(s) 2538
HaeIII GGCC 3 cut(s) 251, 845, 2737
HapII CCGG 1 cut(s) 2357
HhaI GCGC 2 cut(s) 609, 2537
Hin6I GCGC 2 cut(s) 607, 2535
HinP1I GCGC 2 cut(s) 607, 2535
HincII GTYRAC 1 cut(s) 1257
HindII GTYRAC 1 cut(s) 1257
HindIII AAGCTT 1 cut(s) 18
HpaII CCGG 1 cut(s) 2357
Hpy166II GTNNAC 8 cut(s) 206, 335, 421, 1257, 1414, 2014, 2422, 2875
Hpy8I GTNNAC 8 cut(s) 206, 335, 421, 1257, 1414, 2014, 2422, 2875
Hpy99I CGWCG 1 cut(s) 629
HpyAV CCTTC 9 cut(s) 882, 1222, 1509, 1618, 1852, 2061, 2668, 2673, 2852
HpyCH4III ACNGT 5 cut(s) 120, 346, 460, 2621, 2927
HpyCH4IV ACGT 2 cut(s) 202, 2176
HpyF10VI GCNNNNNNNGC 1 cut(s) 620
HpySE526I ACGT 2 cut(s) 202, 2176
HspAI GCGC 2 cut(s) 607, 2535
Ksp22I TGATCA 2 cut(s) 1090, 1845
LmnI GCTCC 1 cut(s) 2359
Lsp1109I GCAGC 4 cut(s) 748, 1064, 2254, 2291
LweI GCATC 9 cut(s) 487, 868, 890, 1612, 2047, 2069, 2279, 2458, 2560
MaeI CTAG 4 cut(s) 231, 281, 702, 1758
MaeII ACGT 2 cut(s) 202, 2176
MaeIII GTNAC 6 cut(s) 346, 970, 1387, 1535, 2359, 2842
MflI RGATCY 7 cut(s) 1026, 1342, 1468, 1948, 2369, 2434, 2484
MhlI GDGCHC 4 cut(s) 685, 940, 2877, 2925
MlsI TGGCCA 2 cut(s) 845, 2737
MluNI TGGCCA 2 cut(s) 845, 2737
MlyI GAGTC 7 cut(s) 892, 1331, 1808, 1871, 1937, 2351, 2512
MmeI TCCRAC 2 cut(s) 1016, 1465
Mox20I TGGCCA 2 cut(s) 845, 2737
Mph1103I ATGCAT 2 cut(s) 883, 2062
MroXI GAANNNNTTC 2 cut(s) 2092, 2759
MscI TGGCCA 2 cut(s) 845, 2737
MseI TTAA 6 cut(s) 237, 831, 1748, 1994, 2103, 2883
MslI CAYNNNNRTG 5 cut(s) 426, 579, 968, 1267, 2184
Msp20I TGGCCA 2 cut(s) 845, 2737
MspA1I CMGCKG 2 cut(s) 1511, 2304
MspI CCGG 1 cut(s) 2357
MspR9I CCNGG 2 cut(s) 247, 1110
Mva1269I GAATGC 4 cut(s) 274, 1679, 1687, 1832
MvaI CCWGG 2 cut(s) 247, 1110
MwoI GCNNNNNNNGC 1 cut(s) 620
NdeI CATATG 1 cut(s) 2864
NlaIV GGNNCC 7 cut(s) 76, 244, 939, 1566, 1740, 1875, 2371
NmuCI GTSAC 2 cut(s) 970, 1387
NsiI ATGCAT 2 cut(s) 883, 2062
NspI RCATGY 2 cut(s) 634, 2189
NspV TTCGAA 2 cut(s) 15, 1043
OliI CACNNNNGTG 1 cut(s) 968
PaqCI CACCTGC 1 cut(s) 2564
PciI ACATGT 2 cut(s) 630, 2185
PctI GAATGC 4 cut(s) 274, 1679, 1687, 1832
PdmI GAANNNNTTC 2 cut(s) 2092, 2759
PfeI GAWTC 9 cut(s) 52, 197, 379, 710, 794, 956, 1460, 1580, 1988
PkrI GCNGC 4 cut(s) 763, 1054, 2269, 2306
PleI GAGTC 7 cut(s) 892, 1330, 1807, 1870, 1936, 2350, 2511
PmaCI CACGTG 1 cut(s) 203
PmlI CACGTG 1 cut(s) 203
PpsI GAGTC 7 cut(s) 892, 1330, 1807, 1870, 1936, 2350, 2511
Ppu21I YACGTR 1 cut(s) 203
PpuMI RGGWCCY 1 cut(s) 2717
PscI ACATGT 2 cut(s) 630, 2185
PshAI GACNNNNGTC 1 cut(s) 1140
Psp5II RGGWCCY 1 cut(s) 2717
Psp6I CCWGG 2 cut(s) 245, 1108
PspCI CACGTG 1 cut(s) 203
PspGI CCWGG 2 cut(s) 245, 1108
PspN4I GGNNCC 7 cut(s) 76, 244, 939, 1566, 1740, 1875, 2371
PspPI GGNCC 4 cut(s) 249, 491, 1505, 2717
PspPPI RGGWCCY 1 cut(s) 2717
PstI CTGCAG 2 cut(s) 766, 1187
PstNI CAGNNNCTG 1 cut(s) 2267
PsuI RGATCY 7 cut(s) 1026, 1342, 1468, 1948, 2369, 2434, 2484
PvuII CAGCTG 2 cut(s) 1511, 2304
RsaI GTAC 6 cut(s) 457, 1107, 1725, 2013, 2184, 2833
RsaNI GTAC 6 cut(s) 456, 1106, 1724, 2012, 2183, 2832
RseI CAYNNNNRTG 5 cut(s) 426, 579, 968, 1267, 2184
SaqAI TTAA 6 cut(s) 237, 831, 1748, 1994, 2103, 2883
SatI GCNGC 4 cut(s) 762, 1053, 2268, 2305
Sau96I GGNCC 4 cut(s) 249, 491, 1505, 2717
SchI GAGTC 7 cut(s) 892, 1331, 1808, 1871, 1937, 2351, 2512
ScrFI CCNGG 2 cut(s) 247, 1110
SduI GDGCHC 4 cut(s) 685, 940, 2877, 2925
SfaNI GCATC 9 cut(s) 487, 868, 890, 1612, 2047, 2069, 2279, 2458, 2560
SfcI CTRYAG 2 cut(s) 762, 1183
SfuI TTCGAA 2 cut(s) 15, 1043
SinI GGWCC 3 cut(s) 491, 1505, 2717
SmiMI CAYNNNNRTG 5 cut(s) 426, 579, 968, 1267, 2184
SmlI CTYRAG 1 cut(s) 1765
SmoI CTYRAG 1 cut(s) 1765
SsiI CCGC 2 cut(s) 211, 1272
SspI AATATT 2 cut(s) 1297, 2378
SspMI CTAG 4 cut(s) 231, 281, 702, 1758
StyD4I CCNGG 2 cut(s) 245, 1108
StyI CCWWGG 2 cut(s) 1479, 1551
TaaI ACNGT 5 cut(s) 120, 346, 460, 2621, 2927
TaiI ACGT 2 cut(s) 205, 2179
TaqI TCGA 5 cut(s) 15, 84, 1043, 2295, 2827
TatI WGTACW 3 cut(s) 455, 2182, 2831
TfiI GAWTC 9 cut(s) 52, 197, 379, 710, 794, 956, 1460, 1580, 1988
Tru1I TTAA 6 cut(s) 237, 831, 1748, 1994, 2103, 2883
Tru9I TTAA 6 cut(s) 237, 831, 1748, 1994, 2103, 2883
TscAI CASTG 9 cut(s) 440, 465, 745, 771, 807, 846, 1594, 2268, 2550
TseFI GTSAC 2 cut(s) 970, 1387
TseI GCWGC 4 cut(s) 761, 1052, 2267, 2304
Tsp45I GTSAC 2 cut(s) 970, 1387
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 9 cut(s) 440, 465, 745, 771, 807, 846, 1594, 2268, 2550
VneI GTGCAC 1 cut(s) 2873
VpaK11BI GGWCC 3 cut(s) 491, 1505, 2717
XapI RAATTY 8 cut(s) 617, 834, 1170, 1494, 2324, 2334, 2756, 2903
XceI RCATGY 2 cut(s) 634, 2189
XcmI CCANNNNNNNNNTGG 2 cut(s) 853, 1685
XmnI GAANNNNTTC 2 cut(s) 2092, 2759
XspI CTAG 4 cut(s) 231, 281, 702, 1758
Zsp2I ATGCAT 2 cut(s) 883, 2062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.