FvH4_5g18040

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002045
Physical Location & Seq
Forward (+)
10368550 .. 10374860
6311 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18040.t1

Sequence Viewer

Length: 3516 bp
ATGCATTGTGCCCTTCGAAGAGCTAATAGCGATTTTCAGAAGGGTGCAGACAGAGGAAGAGATTCCTTATCAAGTAAAGAACAAAGTAGTTTCAGGACATCTTTAGAGGAATGCGAAGTGCCTTCTGTCACATGGAGGAACTCCGATAGTAGGTGTGCTATCTTTAAATTCCTTTCCCTTGAACCTGACGGACGTTGGAGAATTGTGGCACTACCATTGCAGTGTACTGATAATATAAATAATCTGGTATCGGGTGCTCTGGTGAATATGGAAAGTCTTCATCTTGCTTACTCGCCACCAATTAGTCCTTTCAAGTTTAATCGACAGAAGGTGCAGAAGGGTCCTCCTCTTGATGTTGTTTATTCTGTCAAGTCATTTACAAATAGAAGATTTACTGATTCAACTAAGAGACACCAATCTCGAAATAAGACCTTGGTAAATAAAGCTACCAAATGGAATGTTTCCAGGAAATTTCAGAAGTCATTAACTTCCGGTGATTCCTTGGCTATCATTGCAAATGATTCTAATGCAATCATATCGTCAGATGTGGTTAGTAATAGAAAAATAGACAGCATTGTGAAGAGAAGTTCAAGAAAGAAGAGCAGAAAGAAGGGAAAACAGAGCAAGAAAGTTTTATGCAATGTTCGCTCTACTGATCCTGAAGTGATTTCTGAGGAATATGGAAATGTAAGTTCAGCTTCTGAAACCTGCGGTAACAATGAAGGGGATGGACCAGCATTGTCTTCAACTGCACCAGTATTTTTATTGCTAGATGCTAAGAGGACATGTACCTCTTCCCCTGATGAGTTAGGCACTGTGAGTAATCTTGAAAATCAAATTATACTAAAAGATTCTGCATTTCCCATCTTAGATGGGGTGGAGGGTATTCACCACACAAAGGCGAGCGATTGCAGTGATCTCTATACCAAAGGTTACTCTGAGATGCATGACTCCTTCATATTGGATTCGATCTCAATTGGCTCAAACAGTGATGGTAGTATTAATCTTGGTCATGATGAAAAACATGCTGACAAGGAAATTTACAACACAGATATATCTGAACCACCAAATTCGAATTCTAGAAAGGTATATTTCACTCGTCAAAGCTCACTGAATGATTTTGTAAACACCTATAATCACACTGAGGGAGCCAGGCAGTGTACTCATGGTTGTAGCAGTAGTACTGATATGAAATATGTTGTGCCTAACAAGAGAAGTAGGCAAAACAAAGTAGGACAACGAAGTGCAAATGTTCCCAAATCTGGTAGTGTTGGAAATATGCGTACAGGGAAGGAAAATATTCATTCCGTCTGGCAAAAGGTCCAGAAGAATGACGCCAATGATTGCACTGGTGAATTGAAGACAGCAAGTTCTGTTTACTCAAGGCTTGATCTCCCTTTGAAAGAGGCTCCCATGATTAATAGAACCTGTAATTCTGTTGATATAGATGTCTTTTTAAAGAGTGAGAATAGGAAACAGCAAAAAGATAAGGTCTCAAAAAAGTTGAAAAGAAGAAACGCTCCAGCTTTGAAACGAGAGTACAGATGTTATTCCAGGAAAGGATCTCATGCTAGCTTGGCTGGCTCAGATGGTTCTTTGAAGCTTCGGATGGATCAGAGTGACATATCAGATATCTTGACTCAGGCGAAGGACAAGAAAGGGTTAAGTCTTGTTTCGACATCTTGTTCTCAGCCTAGTTGTCCAACAGCTGGATTTCAGACCAGCAAAGTAGAATGCAAGTCTGAATCAGTTCAAAGCATGCAACTCTGTCCAAATGAGATTGGTCATCTCGAAAATGTCTGTAAGACTGTCTCAGTCATGAATGATCAAAATGTAGGAAATGATGATGGTTCAATGCAAAAGATGTCAAATCTACTTCAGATGCAATCTCTAGTATACCTTCCCCATCTACTTCATGATGCAGCAAGTCAAGAAGTACAGAGACAAATCTCTCTTGCAGAGAGCAGCAAGCAAAACCGTAGTAGTTCTGGATCTCTTACGCAGAAGTGGATGCCAATTGGGTTGAAGGATTCTGAGTTGGCAAGCTCTACTAGATCTGAAAGTTCATCCCTTGAACATTCTGATGAGGGAGCTTCTAAGAGATGGACTATTAAAGACACTATTAAAGGCAATGTGGTTTCTAAAGAAGCCGTTGAGTCCACGACTCAGGGTTCTATAGACGTAACTTGTTCTTCTGATGACACCGAGGGCAGGCTTCTTATATCAAATGCAGTCAAAGAGCTGACCAACAACAAGCTTGATGCAGCTAACTACGTGAATAGTTCTGATGTATCCAAAGGGCTAAATGCTTTTGAAGCTTATTCAAACAGATTATTAGAAGCAGTGAGTGATGCTTGTAGGGCACAGCTCGCATCTGAAACTGTTCAAATGATCACTGGTCAACCAATTGCCGAATTTGAAAGACTTCTTTATTATTCATCTCCCGTTATACATCAATCACCTAGCTGCATAAGTTGCCACACTTGCTGCTCACGGAACCTGTCTGACCAGGTTGGTGGAGCGTCATTGTGCAGACATGAATCACCAAATGTCACTTTAAAATGCCTTTGGGAGTGGTATGAGAAATATGGGAGCTATGGGATGGAAATAAGGGGAGAGGAACTTGGGAATTCAAAGAGATTGGGTACTGATTGTTTCGCATTTCGTGCTTATTTTGTCCCTTATTTGTCAGGAATTCAGCTTTTCAAGAATGGTAGGGGTACTGGTGCTGGAGATATTAACAATGGACTTCACAGTGATGAAGTGTTAGACCCATGCCCGAATGGTGAGATATCCAGAAAATCCTCTAGCATTGGTGGTCTTCCGATATATTCACTACTTTTTTCTCAGCCTGATTGTAAGGAAGATGCAATCACCCCACCACTTGTAAACGAATTGGCTATTCCTGAAGCTTTTGCCAAAGATGTACTTGTTCAATCAGCTGACACAAAATATTTCAGTGATATAGAGCTGCTTTTAGAATATTTTGAATATGAACAACCTCGACAGAGACGTCCTCTATATGACAAGATAAAAGAGCTGGTTAGAGGTGATGGAAACTCACATTCAAAAGTGTATGGCGATCCAACTAAACTAGACTCTATAAATCTAAATGATCTACATCCCAGATCTTGGTACTCTGTGGCATGGTATCCTATTTACAGGATACCTGATGGTAACTTCCGAGCAGCATTTTTGACTTACCATTCACTTGGTTGTTTGGTTCGTAGAAGTGCCAACTTTGAGTCTCCTAGTATGGATGACAGTATTGTATCTCCAGTTGTGGGCCTTCAGAGTTACAACGCTCAGAGTGAATGCTGGTTTCAGCTAAGGCCCTCAGCACCAGCACAGACAATAGTAACAAACTTAAACCCTTGTGGAGTACTGGAAGAGCGTTTGAGGACACTTGAGGAGACAGCATCTGTTATGGCAAGAGCTGTTGTGAGCAAAGGAGGTGTGACATCTGTAAACATGCATCCAGACTATGAATTTTTTCTCTCTAGGCGGCGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

1172

Amino Acids

129.76

Weight (kDa)

8.34

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 804 - 1165 3.3e-61 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3012
AatII GACGTC 1 cut(s) 3016
Acc36I ACCTGC 1 cut(s) 716
AccB7I CCANNNNNTGG 2 cut(s) 1709, 3132
AccI GTMKAC 1 cut(s) 1896
AciI CCGC 2 cut(s) 711, 3505
AclWI GGATC 5 cut(s) 650, 1570, 1620, 1999, 3077
AcsI RAATTY 9 cut(s) 167, 470, 1038, 1069, 1075, 2414, 2629, 2694, 3488
AcuI CTGAAG 4 cut(s) 681, 1862, 2928, 3275
AcyI GRCGYC 2 cut(s) 1335, 3013
AfiI CCNNNNNNNGG 7 cut(s) 150, 898, 1262, 1709, 2211, 3132, 3284
AflIII ACRYGT 1 cut(s) 785
AjnI CCWGG 4 cut(s) 464, 1151, 1553, 2508
AjuI GAANNNNNNNTTGG 2 cut(s) 1060, 1092
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 7 cut(s) 403, 1498, 1816, 1936, 3004, 3252, 3407
AlwI GGATC 5 cut(s) 650, 1570, 1620, 1999, 3077
AlwNI CAGNNNCTG 2 cut(s) 701, 3422
AoxI GGCC 2 cut(s) 3286, 3332
ApeKI GCWGC 7 cut(s) 1922, 1965, 2264, 2466, 2487, 2971, 3188
ApoI RAATTY 9 cut(s) 167, 470, 1038, 1069, 1075, 2414, 2629, 2694, 3488
AseI ATTAAT 2 cut(s) 1002, 1419
Asp700I GAANNNNTTC 7 cut(s) 61, 276, 1299, 1749, 2382, 2424, 3492
AspLEI GCGC 1 cut(s) 3510
AspS9I GGNCC 5 cut(s) 341, 731, 1321, 3286, 3333
AsuHPI GGTGA 9 cut(s) 274, 506, 881, 1364, 2451, 2535, 2798, 2866, 3062
AsuII TTCGAA 2 cut(s) 16, 1073
AsuNHI GCTAGC 1 cut(s) 1571
AvaII GGWCC 3 cut(s) 341, 731, 1321
BaeGI GKGCMC 2 cut(s) 13, 2365
BbsI GAAGAC 4 cut(s) 269, 735, 1367, 2813
Bbv12I GWGCWC 1 cut(s) 259
BbvCI CCTCAGC 1 cut(s) 3337
BbvI GCAGC 7 cut(s) 1934, 1977, 2276, 2453, 2474, 2958, 3200
BceAI ACGGC 1 cut(s) 2135
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BciT130I CCWGG 4 cut(s) 466, 1153, 1555, 2510
BciVI GTATCC 3 cut(s) 2302, 3159, 3162
BclI TGATCA 2 cut(s) 1825, 2391
BcoDI GTCTC 7 cut(s) 403, 1498, 1816, 1936, 3004, 3252, 3407
BfmI CTRYAG 1 cut(s) 2175
BfoI RGCGCY 1 cut(s) 3511
BfuAI ACCTGC 1 cut(s) 716
BfuI GTATCC 3 cut(s) 2302, 3159, 3162
BglII AGATCT 2 cut(s) 2054, 3128
BisI GCNGC 8 cut(s) 1923, 1966, 2265, 2467, 2488, 2972, 3189, 3506
BlsI GCNGC 8 cut(s) 1924, 1967, 2266, 2468, 2489, 2973, 3190, 3507
BmcAI AGTACT 2 cut(s) 1183, 3384
Bme1390I CCNGG 4 cut(s) 466, 1153, 1555, 2510
Bme18I GGWCC 3 cut(s) 341, 731, 1321
BmgT120I GGNCC 5 cut(s) 341, 731, 1321, 3286, 3333
BmiI GGNNCC 4 cut(s) 342, 1150, 1410, 2498
BmrFI CCNGG 4 cut(s) 466, 1153, 1555, 2510
BmtI GCTAGC 1 cut(s) 1575
BpiI GAAGAC 4 cut(s) 269, 735, 1367, 2813
BplI GAGNNNNNCTC 2 cut(s) 3001, 3033
BpmI CTGGAG 3 cut(s) 1506, 2751, 3261
Bpu10I CCTNAGC 2 cut(s) 3329, 3337
Bpu14I TTCGAA 2 cut(s) 16, 1073
BpuEI CTTGAG 2 cut(s) 1366, 3428
BsaAI YACGTR 1 cut(s) 2275
BsaBI GATNNNNATC 1 cut(s) 3120
BsaHI GRCGYC 2 cut(s) 1335, 3013
BsaI GGTCTC 1 cut(s) 1498
BsaJI CCNNGG 3 cut(s) 432, 501, 2205
BsaWI WCCGGW 1 cut(s) 491
Bsc4I CCNNNNNNNGG 7 cut(s) 150, 898, 1262, 1709, 2211, 3132, 3284
Bse1I ACTGG 6 cut(s) 755, 1354, 2401, 2728, 3278, 3390
Bse3DI GCAATG 4 cut(s) 215, 510, 646, 2137
Bse8I GATNNNNATC 1 cut(s) 3120
BseBI CCWGG 4 cut(s) 466, 1153, 1555, 2510
BseDI CCNNGG 3 cut(s) 432, 501, 2205
BseGI GGATG 8 cut(s) 733, 1614, 2016, 2066, 2607, 3121, 3265, 3475
BseJI GATNNNNATC 1 cut(s) 3120
BseLI CCNNNNNNNGG 7 cut(s) 150, 898, 1262, 1709, 2211, 3132, 3284
BseMI GCAATG 4 cut(s) 215, 510, 646, 2137
BseNI ACTGG 6 cut(s) 755, 1354, 2401, 2728, 3278, 3390
BseRI GAGGAG 2 cut(s) 336, 3425
BseSI GKGCMC 2 cut(s) 13, 2365
BseXI GCAGC 7 cut(s) 1934, 1977, 2276, 2453, 2474, 2958, 3200
BsgI GTGCAG 4 cut(s) 66, 353, 735, 2551
BshFI GGCC 2 cut(s) 3288, 3334
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 1 cut(s) 492
BslFI GGGAC 1 cut(s) 2663
BslI CCNNNNNNNGG 7 cut(s) 150, 898, 1262, 1709, 2211, 3132, 3284
BsmAI GTCTC 7 cut(s) 403, 1498, 1816, 1936, 3004, 3252, 3407
BsmBI CGTCTC 1 cut(s) 3004
BsmFI GGGAC 1 cut(s) 2663
BsmI GAATGC 3 cut(s) 116, 1739, 3320
BsnI GGCC 2 cut(s) 3288, 3334
Bso31I GGTCTC 1 cut(s) 1498
Bsp119I TTCGAA 2 cut(s) 16, 1073
Bsp1286I GDGCHC 3 cut(s) 13, 259, 2365
BspACI CCGC 2 cut(s) 711, 3505
BspANI GGCC 2 cut(s) 3288, 3334
BspHI TCATGA 3 cut(s) 1012, 1818, 1915
BspLI GGNNCC 4 cut(s) 342, 1150, 1410, 2498
BspMI ACCTGC 1 cut(s) 716
BspOI GCTAGC 1 cut(s) 1575
BspPI GGATC 5 cut(s) 650, 1570, 1620, 1999, 3077
BspQI GCTCTTC 3 cut(s) 13, 593, 3384
BspT104I TTCGAA 2 cut(s) 16, 1073
BspTNI GGTCTC 1 cut(s) 1498
BsrDI GCAATG 4 cut(s) 215, 510, 646, 2137
BsrI ACTGG 6 cut(s) 755, 1354, 2401, 2728, 3278, 3390
BssECI CCNNGG 3 cut(s) 432, 501, 2205
BssNAI GTATAC 1 cut(s) 1897
BssNI GRCGYC 2 cut(s) 1335, 3013
BssT1I CCWWGG 2 cut(s) 432, 501
Bst1107I GTATAC 1 cut(s) 1897
Bst2UI CCWGG 4 cut(s) 466, 1153, 1555, 2510
Bst4CI ACNGT 7 cut(s) 817, 989, 1810, 1979, 2383, 2756, 3266
Bst6I CTCTTC 6 cut(s) 13, 52, 575, 593, 799, 3384
BstACI GRCGYC 2 cut(s) 1335, 3013
BstAPI GCANNNNNTGC 2 cut(s) 2475, 2666
BstBAI YACGTR 1 cut(s) 2275
BstBI TTCGAA 2 cut(s) 16, 1073
BstC8I GCNNGC 8 cut(s) 904, 1573, 1582, 1760, 1970, 2044, 2213, 2370
BstF5I GGATG 8 cut(s) 733, 1614, 2016, 2066, 2607, 3121, 3265, 3475
BstH2I RGCGCY 1 cut(s) 3511
BstHHI GCGC 1 cut(s) 3510
BstMAI GTCTC 7 cut(s) 403, 1498, 1816, 1936, 3004, 3252, 3407
BstNI CCWGG 4 cut(s) 466, 1153, 1555, 2510
BstNSI RCATGY 4 cut(s) 789, 1028, 1762, 3475
BstSCI CCNGG 4 cut(s) 464, 1151, 1553, 2508
BstSFI CTRYAG 1 cut(s) 2175
BstSLI GKGCMC 2 cut(s) 13, 2365
BstV1I GCAGC 7 cut(s) 1934, 1977, 2276, 2453, 2474, 2958, 3200
BstV2I GAAGAC 4 cut(s) 269, 735, 1367, 2813
BstX2I RGATCY 4 cut(s) 1562, 1991, 2054, 3128
BstXI CCANNNNNNTGG 2 cut(s) 2516, 3212
BstYI RGATCY 4 cut(s) 1562, 1991, 2054, 3128
BstZ17I GTATAC 1 cut(s) 1897
BsuI GTATCC 3 cut(s) 2302, 3159, 3162
BsuRI GGCC 2 cut(s) 3288, 3334
BtsCI GGATG 8 cut(s) 733, 1614, 2016, 2066, 2607, 3121, 3265, 3475
BtsI GCAGTG 4 cut(s) 227, 919, 1163, 2349
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 8 cut(s) 904, 1573, 1582, 1760, 1970, 2044, 2213, 2370
CaiI CAGNNNCTG 2 cut(s) 701, 3422
CciI TCATGA 3 cut(s) 1012, 1818, 1915
CfoI GCGC 1 cut(s) 3510
Cfr13I GGNCC 5 cut(s) 341, 731, 1321, 3286, 3333
CseI GACGC 2 cut(s) 1343, 2511
CsiI ACCWGGT 1 cut(s) 2508
DraI TTTAAA 3 cut(s) 166, 1458, 2559
DrdI GACNNNNNNGTC 1 cut(s) 3012
DseDI GACNNNNNNGTC 1 cut(s) 3012
Eam1104I CTCTTC 6 cut(s) 13, 52, 575, 593, 799, 3384
EarI CTCTTC 6 cut(s) 13, 52, 575, 593, 799, 3384
Eco130I CCWWGG 2 cut(s) 432, 501
Eco31I GGTCTC 1 cut(s) 1498
Eco32I GATATC 2 cut(s) 1633, 2793
Eco47I GGWCC 3 cut(s) 341, 731, 1321
Eco57I CTGAAG 4 cut(s) 681, 1862, 2928, 3275
EcoO109I RGGNCCY 2 cut(s) 341, 3333
EcoRI GAATTC 3 cut(s) 1075, 2629, 2694
EcoRII CCWGG 4 cut(s) 464, 1151, 1553, 2508
EcoRV GATATC 2 cut(s) 1633, 2793
EcoT14I CCWWGG 2 cut(s) 432, 501
EcoT22I ATGCAT 3 cut(s) 6, 948, 3477
ErhI CCWWGG 2 cut(s) 432, 501
Esp3I CGTCTC 1 cut(s) 3004
FalI AAGNNNNNCTT 4 cut(s) 682, 714, 2913, 2945
FaqI GGGAC 1 cut(s) 2663
FbaI TGATCA 2 cut(s) 1825, 2391
FblI GTMKAC 1 cut(s) 1896
Fnu4HI GCNGC 8 cut(s) 1923, 1966, 2265, 2467, 2488, 2972, 3189, 3506
FokI GGATG 8 cut(s) 740, 1621, 2023, 2053, 2614, 3108, 3272, 3462
Fsp4HI GCNGC 8 cut(s) 1923, 1966, 2265, 2467, 2488, 2972, 3189, 3506
GlaI GCGC 1 cut(s) 3509
GluI GCNGC 8 cut(s) 1923, 1966, 2265, 2467, 2488, 2972, 3189, 3506
GsuI CTGGAG 3 cut(s) 1506, 2751, 3261
HaeII RGCGCY 1 cut(s) 3511
HaeIII GGCC 2 cut(s) 3288, 3334
HapII CCGG 1 cut(s) 492
HgaI GACGC 2 cut(s) 1343, 2511
HhaI GCGC 1 cut(s) 3510
Hin1I GRCGYC 2 cut(s) 1335, 3013
Hin6I GCGC 1 cut(s) 3508
HinP1I GCGC 1 cut(s) 3508
HincII GTYRAC 1 cut(s) 2402
HindII GTYRAC 1 cut(s) 2402
HindIII AAGCTT 4 cut(s) 1601, 2255, 2316, 2910
HpaII CCGG 1 cut(s) 492
HphI GGTGA 9 cut(s) 274, 506, 881, 1364, 2451, 2535, 2798, 2866, 3062
Hpy166II GTNNAC 9 cut(s) 225, 1126, 1161, 1378, 1897, 2160, 2402, 2890, 3469
Hpy8I GTNNAC 9 cut(s) 225, 1126, 1161, 1378, 1897, 2160, 2402, 2890, 3469
HpyCH4III ACNGT 7 cut(s) 817, 989, 1810, 1979, 2383, 2756, 3266
HpyCH4IV ACGT 4 cut(s) 193, 2181, 2274, 3013
HpySE526I ACGT 4 cut(s) 193, 2181, 2274, 3013
Hsp92I GRCGYC 2 cut(s) 1335, 3013
HspAI GCGC 1 cut(s) 3508
Ksp22I TGATCA 2 cut(s) 1825, 2391
LguI GCTCTTC 3 cut(s) 13, 593, 3384
LmnI GCTCC 6 cut(s) 1148, 1414, 1525, 2090, 2519, 2592
Lsp1109I GCAGC 7 cut(s) 1934, 1977, 2276, 2453, 2474, 2958, 3200
MabI ACCWGGT 1 cut(s) 2508
MaeII ACGT 4 cut(s) 193, 2181, 2274, 3013
MfeI CAATTG 3 cut(s) 975, 2016, 2406
MflI RGATCY 4 cut(s) 1562, 1991, 2054, 3128
MhlI GDGCHC 3 cut(s) 13, 259, 2365
MlyI GAGTC 6 cut(s) 944, 1633, 2158, 2165, 3092, 3254
MmeI TCCRAC 4 cut(s) 176, 1252, 1727, 3110
Mph1103I ATGCAT 3 cut(s) 6, 948, 3477
MroXI GAANNNNTTC 7 cut(s) 61, 276, 1299, 1749, 2382, 2424, 3492
MslI CAYNNNNRTG 3 cut(s) 220, 2082, 2757
MspA1I CMGCKG 2 cut(s) 1709, 2942
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 4 cut(s) 466, 1153, 1555, 2510
MunI CAATTG 3 cut(s) 975, 2016, 2406
Mva1269I GAATGC 3 cut(s) 116, 1739, 3320
MvaI CCWGG 4 cut(s) 466, 1153, 1555, 2510
NheI GCTAGC 1 cut(s) 1571
NlaIV GGNNCC 4 cut(s) 342, 1150, 1410, 2498
NmuCI GTSAC 4 cut(s) 127, 1619, 2551, 3457
NsiI ATGCAT 3 cut(s) 6, 948, 3477
NspI RCATGY 4 cut(s) 789, 1028, 1762, 3475
NspV TTCGAA 2 cut(s) 16, 1073
PaeI GCATGC 1 cut(s) 1762
PagI TCATGA 3 cut(s) 1012, 1818, 1915
PciI ACATGT 1 cut(s) 785
PciSI GCTCTTC 3 cut(s) 13, 593, 3384
PcsI WCGNNNNNNNCGW 1 cut(s) 3010
PctI GAATGC 3 cut(s) 116, 1739, 3320
PdmI GAANNNNTTC 7 cut(s) 61, 276, 1299, 1749, 2382, 2424, 3492
PfeI GAWTC 9 cut(s) 62, 398, 497, 521, 851, 965, 1745, 2030, 2540
PflMI CCANNNNNTGG 2 cut(s) 1709, 3132
PfoI TCCNGGA 2 cut(s) 464, 1553
PkrI GCNGC 8 cut(s) 1924, 1967, 2266, 2468, 2489, 2973, 3190, 3507
PleI GAGTC 6 cut(s) 944, 1633, 2158, 2164, 3092, 3253
PpsI GAGTC 6 cut(s) 944, 1633, 2158, 2164, 3092, 3253
Ppu21I YACGTR 1 cut(s) 2275
PpuMI RGGWCCY 1 cut(s) 341
PscI ACATGT 1 cut(s) 785
PshBI ATTAAT 2 cut(s) 1002, 1419
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 4 cut(s) 464, 1151, 1553, 2508
PspGI CCWGG 4 cut(s) 464, 1151, 1553, 2508
PspN4I GGNNCC 4 cut(s) 342, 1150, 1410, 2498
PspPI GGNCC 5 cut(s) 341, 731, 1321, 3286, 3333
PspPPI RGGWCCY 1 cut(s) 341
PstNI CAGNNNCTG 2 cut(s) 701, 3422
PsuI RGATCY 4 cut(s) 1562, 1991, 2054, 3128
PvuII CAGCTG 2 cut(s) 1709, 2942
RseI CAYNNNNRTG 3 cut(s) 220, 2082, 2757
SapI GCTCTTC 3 cut(s) 13, 593, 3384
SatI GCNGC 8 cut(s) 1923, 1966, 2265, 2467, 2488, 2972, 3189, 3506
Sau96I GGNCC 5 cut(s) 341, 731, 1321, 3286, 3333
ScaI AGTACT 2 cut(s) 1183, 3384
SchI GAGTC 6 cut(s) 944, 1633, 2158, 2165, 3092, 3254
ScrFI CCNGG 4 cut(s) 466, 1153, 1555, 2510
SduI GDGCHC 3 cut(s) 13, 259, 2365
SexAI ACCWGGT 1 cut(s) 2508
SfcI CTRYAG 1 cut(s) 2175
SfuI TTCGAA 2 cut(s) 16, 1073
SinI GGWCC 3 cut(s) 341, 731, 1321
SmiMI CAYNNNNRTG 3 cut(s) 220, 2082, 2757
SmlI CTYRAG 2 cut(s) 1381, 3407
SmoI CTYRAG 2 cut(s) 1381, 3407
SphI GCATGC 1 cut(s) 1762
SsiI CCGC 2 cut(s) 711, 3505
SspI AATATT 3 cut(s) 1300, 2954, 2984
StyD4I CCNGG 4 cut(s) 464, 1151, 1553, 2508
StyI CCWWGG 2 cut(s) 432, 501
TaaI ACNGT 7 cut(s) 817, 989, 1810, 1979, 2383, 2756, 3266
TaiI ACGT 4 cut(s) 196, 2184, 2277, 3016
TaqI TCGA 8 cut(s) 16, 322, 421, 968, 1073, 1676, 1791, 3004
TatI WGTACW 7 cut(s) 224, 1160, 1181, 1539, 1936, 2926, 3382
TauI GCSGC 1 cut(s) 3508
TfiI GAWTC 9 cut(s) 62, 398, 497, 521, 851, 965, 1745, 2030, 2540
TseFI GTSAC 4 cut(s) 127, 1619, 2551, 3457
TseI GCWGC 7 cut(s) 1922, 1965, 2264, 2466, 2487, 2971, 3188
Tsp45I GTSAC 4 cut(s) 127, 1619, 2551, 3457
TspGWI ACGGA 3 cut(s) 204, 1297, 2509
Van91I CCANNNNNTGG 2 cut(s) 1709, 3132
VpaK11BI GGWCC 3 cut(s) 341, 731, 1321
VspI ATTAAT 2 cut(s) 1002, 1419
XapI RAATTY 9 cut(s) 167, 470, 1038, 1069, 1075, 2414, 2629, 2694, 3488
XbaI TCTAGA 1 cut(s) 1079
XceI RCATGY 4 cut(s) 789, 1028, 1762, 3475
XmiI GTMKAC 1 cut(s) 1896
XmnI GAANNNNTTC 7 cut(s) 61, 276, 1299, 1749, 2382, 2424, 3492
ZraI GACGTC 1 cut(s) 3014
ZrmI AGTACT 2 cut(s) 1183, 3384
Zsp2I ATGCAT 3 cut(s) 6, 948, 3477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.