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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 129 |
| AgsI | TTSAA | 2 cut(s) | 44, 112 |
| Alw26I | GTCTC | 1 cut(s) | 32 |
| BcoDI | GTCTC | 1 cut(s) | 32 |
| BfuAI | ACCTGC | 1 cut(s) | 129 |
| BpmI | CTGGAG | 1 cut(s) | 118 |
| BseMII | CTCAG | 1 cut(s) | 75 |
| BsgI | GTGCAG | 1 cut(s) | 68 |
| BsmAI | GTCTC | 1 cut(s) | 32 |
| Bsp143I | GATC | 1 cut(s) | 75 |
| BspCNI | CTCAG | 1 cut(s) | 76 |
| BspMI | ACCTGC | 1 cut(s) | 129 |
| BssMI | GATC | 1 cut(s) | 75 |
| BstDEI | CTNAG | 2 cut(s) | 31, 84 |
| BstKTI | GATC | 1 cut(s) | 78 |
| BstMAI | GTCTC | 1 cut(s) | 32 |
| BstMBI | GATC | 1 cut(s) | 75 |
| BveI | ACCTGC | 1 cut(s) | 129 |
| CviJI | RGCY | 1 cut(s) | 99 |
| CviKI_1 | RGCY | 1 cut(s) | 99 |
| DdeI | CTNAG | 2 cut(s) | 31, 84 |
| DpnI | GATC | 1 cut(s) | 77 |
| DpnII | GATC | 1 cut(s) | 75 |
| FaiI | YATR | 4 cut(s) | 66, 68, 144, 182 |
| GsuI | CTGGAG | 1 cut(s) | 118 |
| Hpy188I | TCNGA | 1 cut(s) | 61 |
| Hpy188III | TCNNGA | 1 cut(s) | 79 |
| HpyCH4V | TGCA | 2 cut(s) | 49, 192 |
| HpyF3I | CTNAG | 2 cut(s) | 31, 84 |
| Kzo9I | GATC | 1 cut(s) | 75 |
| LpnPI | CCDG | 3 cut(s) | 64, 124, 148 |
| MaeIII | GTNAC | 1 cut(s) | 128 |
| MalI | GATC | 1 cut(s) | 77 |
| MboI | GATC | 1 cut(s) | 75 |
| MnlI | CCTC | 1 cut(s) | 48 |
| MseI | TTAA | 1 cut(s) | 14 |
| NdeII | GATC | 1 cut(s) | 75 |
| SaqAI | TTAA | 1 cut(s) | 14 |
| Sau3AI | GATC | 1 cut(s) | 75 |
| SetI | ASST | 2 cut(s) | 59, 143 |
| SgeI | CNNG | 5 cut(s) | 91, 135, 147, 151, 159 |
| Tru1I | TTAA | 1 cut(s) | 14 |
| Tru9I | TTAA | 1 cut(s) | 14 |
| TspDTI | ATGAA | 1 cut(s) | 186 |