FvH4_5g18190

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006034
Physical Location & Seq
Forward (+)
10461877 .. 10465181
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18190.t1

Sequence Viewer

Length: 1209 bp
ATGAGCAGTAAATCATTATCACCAGTATCAGCACTGGTATCTGTAACAGTTATTGGGCATAACAGTACCAGTGTTATGGAGAATCTTCATAACACTACAGCTGCAACACCAAAGGCTGGTGGTGTTCATCACAGTTATCATCATCATGGATTGGTCAGGAAGATTATAAAGATTGCTACGGTGTGTGTTGCTGTTACTCTGGTTTCCCTTCTCTTCTATATCTGTGTCTTCCCCGGCGGTGGATCTTATTATGATCCTTTGTTCTCAGCTAAATCTCAGGATTCCTTGGATGCAGTTCTGAGAAATGCATCCATGAAGGACAAGACCATAATCGTCACAACACTGAATGATGCGTGGGCAGAACCCAACTCCATATTCGATCTGTTTCTCGAGAGCTTTCGTATCGGAAACAACACGAAAAGACTACTGAAGCATCTGGTAGTGATCTGCTTGGACCAAAAAGCATACGCTCGTTGCGTGGCTCTTCACCCACATTGCTACCAACTCTACACTGAAGGTGCCAACTTCACCAGCGAGGCAGCCTTCATGTCTTCCGATTACTTGCACATGATGTGGAGAAGAATCCAGTTTTTGACTTTCATTCTCGAAAAAGGATACAGCTTTGTCTTCACGGACACTGACATAATGTGGCTTAGGGATCCATTCCCAAAATTCTTCCCAGATGCAGACTTCCAAATTGCCTGTGACTATTTCCAAGGCGATTCTTACGATGTGAACAACCTTCCCAACGGAGGATTTACCTATGTAATCTCTAATAAACGAACCACCAAATTCTACAACTTTTGGTACTTCTCAAGGCAGGCTTATCCAAACAACCACGACCAGGACGTCCTCAACAAGATCAAATCTGATCCCTTCATCACCACCATCGGATTGAAAATGAGATTCTTGGACACCAAGTACTTTGGCGGCTTCTGTCAACCCAGTAAAGATCTTAACCTGGTTTGCACAATGCATGCTAATTGCTGCGTTGGTCTCGATAACAAAGTCAATGACCTCAAAATTCTGCTACAAGATTGGAGAAACTACATGGCACTGCCTCCTTCTTCATCATCTTCTTCTTCCTTGTCTTCCAGTAGTCCTAGTTCTAATTCCACAACGACTCCAACAACTATGCCCTCGTGGACAGTCCCACAGAACTGCAGTACGTCCTTCCAACGCCAACGCTCAGGCCGGCACTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

403

Amino Acids

45.32

Weight (kDa)

8.65

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 142 - 340 4.9e-68 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 167
AasI GACNNNNNNGTC 1 cut(s) 848
AatII GACGTC 1 cut(s) 852
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 116
AciI CCGC 2 cut(s) 237, 930
AclWI GGATC 5 cut(s) 248, 250, 653, 666, 866
AcsI RAATTY 3 cut(s) 671, 791, 1023
AcuI CTGAAG 2 cut(s) 449, 534
AcyI GRCGYC 1 cut(s) 849
AfaI GTAC 4 cut(s) 67, 809, 923, 1168
AfiI CCNNNNNNNGG 4 cut(s) 116, 239, 752, 844
AgsI TTSAA 1 cut(s) 898
AjnI CCWGG 2 cut(s) 843, 960
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 4 cut(s) 101, 269, 396, 621
AluI AGCT 4 cut(s) 101, 269, 396, 621
Alw26I GTCTC 1 cut(s) 1001
AlwI GGATC 5 cut(s) 248, 250, 653, 666, 866
Ama87I CYCGRG 1 cut(s) 389
AoxI GGCC 1 cut(s) 1192
ApeKI GCWGC 3 cut(s) 101, 539, 987
ApoI RAATTY 3 cut(s) 671, 791, 1023
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 4 cut(s) 12, 479, 520, 874
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 1 cut(s) 454
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 518
BauI CACGAG 1 cut(s) 1141
BbsI GAAGAC 4 cut(s) 220, 543, 619, 1083
BbvI GCAGC 3 cut(s) 88, 551, 974
BccI CCATC 1 cut(s) 896
BciT130I CCWGG 2 cut(s) 845, 962
BciVI GTATCC 1 cut(s) 608
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 1001
BfaI CTAG 1 cut(s) 1104
BfmI CTRYAG 2 cut(s) 96, 1162
BfuI GTATCC 1 cut(s) 608
BglII AGATCT 1 cut(s) 952
BisI GCNGC 4 cut(s) 102, 540, 931, 988
BlsI GCNGC 4 cut(s) 103, 541, 932, 989
BmcAI AGTACT 1 cut(s) 923
Bme1390I CCNGG 3 cut(s) 234, 845, 962
Bme18I GGWCC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 2 cut(s) 520, 660
BmrFI CCNGG 3 cut(s) 234, 845, 962
BmrI ACTGGG 1 cut(s) 939
BmsI GCATC 5 cut(s) 280, 317, 340, 442, 673
BmuI ACTGGG 1 cut(s) 939
BpiI GAAGAC 4 cut(s) 220, 543, 619, 1083
Bpu10I CCTNAGC 2 cut(s) 653, 1189
BpuEI CTTGAG 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 849
BsaI GGTCTC 1 cut(s) 1001
BsaJI CCNNGG 3 cut(s) 232, 285, 715
BsaXI ACNNNNNCTCC 2 cut(s) 1108, 1138
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 239, 752, 844
Bse118I RCCGGY 1 cut(s) 1194
Bse1I ACTGG 6 cut(s) 23, 39, 69, 586, 945, 1095
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 2 cut(s) 845, 962
BseDI CCNNGG 3 cut(s) 232, 285, 715
BseGI GGATG 2 cut(s) 295, 308
BseLI CCNNNNNNNGG 4 cut(s) 116, 239, 752, 844
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 4 cut(s) 279, 290, 290, 1203
BseNI ACTGG 6 cut(s) 23, 39, 69, 586, 945, 1095
BseXI GCAGC 3 cut(s) 88, 551, 974
BshFI GGCC 1 cut(s) 1194
BshNI GGYRCC 1 cut(s) 518
BsiHKCI CYCGRG 1 cut(s) 389
BsiSI CCGG 2 cut(s) 234, 1195
BslFI GGGAC 1 cut(s) 1136
BslI CCNNNNNNNGG 4 cut(s) 116, 239, 752, 844
BsmAI GTCTC 1 cut(s) 1001
BsmFI GGGAC 1 cut(s) 1136
BsnI GGCC 1 cut(s) 1194
Bso31I GGTCTC 1 cut(s) 1001
BsoBI CYCGRG 1 cut(s) 389
Bsp143I GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
BspACI CCGC 2 cut(s) 237, 930
BspANI GGCC 1 cut(s) 1194
BspCNI CTCAG 4 cut(s) 278, 289, 291, 1202
BspLI GGNNCC 2 cut(s) 520, 660
BspMAI CTGCAG 1 cut(s) 1166
BspPI GGATC 5 cut(s) 248, 250, 653, 666, 866
BspQI GCTCTTC 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 1001
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 1194
BsrI ACTGG 6 cut(s) 23, 39, 69, 586, 945, 1095
BssAI RCCGGY 1 cut(s) 1194
BssECI CCNNGG 3 cut(s) 232, 285, 715
BssMI GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
BssNI GRCGYC 1 cut(s) 849
BssSI CACGAG 1 cut(s) 1141
BssT1I CCWWGG 2 cut(s) 285, 715
Bst2BI CACGAG 1 cut(s) 1141
Bst2UI CCWGG 2 cut(s) 845, 962
Bst4CI ACNGT 5 cut(s) 49, 65, 134, 181, 1150
Bst6I CTCTTC 2 cut(s) 218, 489
BstACI GRCGYC 1 cut(s) 849
BstC8I GCNNGC 3 cut(s) 822, 978, 1196
BstDEI CTNAG 5 cut(s) 265, 276, 299, 653, 1189
BstF5I GGATG 2 cut(s) 295, 308
BstKTI GATC 8 cut(s) 245, 256, 382, 447, 661, 864, 874, 955
BstMAI GTCTC 1 cut(s) 1001
BstMBI GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
BstNI CCWGG 2 cut(s) 845, 962
BstNSI RCATGY 1 cut(s) 980
BstSCI CCNGG 3 cut(s) 232, 843, 960
BstSFI CTRYAG 2 cut(s) 96, 1162
BstV1I GCAGC 3 cut(s) 88, 551, 974
BstV2I GAAGAC 4 cut(s) 220, 543, 619, 1083
BstX2I RGATCY 3 cut(s) 242, 658, 952
BstXI CCANNNNNNTGG 1 cut(s) 76
BstYI RGATCY 3 cut(s) 242, 658, 952
BsuI GTATCC 1 cut(s) 608
BsuRI GGCC 1 cut(s) 1194
BtsCI GGATG 2 cut(s) 295, 308
BtsI GCAGTG 1 cut(s) 1055
BtsIMutI CAGTG 6 cut(s) 32, 76, 341, 510, 636, 1055
Cac8I GCNNGC 3 cut(s) 822, 978, 1196
Cfr10I RCCGGY 1 cut(s) 1194
Cfr13I GGNCC 1 cut(s) 454
CsiI ACCWGGT 1 cut(s) 960
Csp6I GTAC 4 cut(s) 66, 808, 922, 1167
CviAII CATG 6 cut(s) 146, 313, 547, 568, 977, 1051
CviQI GTAC 4 cut(s) 66, 808, 922, 1167
DdeI CTNAG 5 cut(s) 265, 276, 299, 653, 1189
DpnI GATC 8 cut(s) 244, 255, 381, 446, 660, 863, 873, 954
DpnII GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
DrdI GACNNNNNNGTC 1 cut(s) 848
DseDI GACNNNNNNGTC 1 cut(s) 848
Eam1104I CTCTTC 2 cut(s) 218, 489
EarI CTCTTC 2 cut(s) 218, 489
Eco130I CCWWGG 2 cut(s) 285, 715
Eco31I GGTCTC 1 cut(s) 1001
Eco47I GGWCC 1 cut(s) 454
Eco57I CTGAAG 2 cut(s) 449, 534
Eco88I CYCGRG 1 cut(s) 389
EcoRII CCWGG 2 cut(s) 843, 960
EcoT14I CCWWGG 2 cut(s) 285, 715
EcoT22I ATGCAT 2 cut(s) 310, 978
ErhI CCWWGG 2 cut(s) 285, 715
FaeI CATG 6 cut(s) 149, 316, 550, 571, 980, 1054
FalI AAGNNNNNCTT 2 cut(s) 808, 840
FaqI GGGAC 1 cut(s) 1136
FatI CATG 6 cut(s) 145, 312, 546, 567, 976, 1050
Fnu4HI GCNGC 4 cut(s) 102, 540, 931, 988
FokI GGATG 2 cut(s) 295, 302
Fsp4HI GCNGC 4 cut(s) 102, 540, 931, 988
FspBI CTAG 1 cut(s) 1104
GluI GCNGC 4 cut(s) 102, 540, 931, 988
HaeIII GGCC 1 cut(s) 1194
HapII CCGG 2 cut(s) 234, 1195
Hin1I GRCGYC 1 cut(s) 849
Hin1II CATG 6 cut(s) 149, 316, 550, 571, 980, 1054
HincII GTYRAC 1 cut(s) 941
HindII GTYRAC 1 cut(s) 941
HinfI GANTC 6 cut(s) 82, 281, 582, 722, 906, 1123
HpaII CCGG 2 cut(s) 234, 1195
HphI GGTGA 4 cut(s) 12, 479, 520, 874
Hpy166II GTNNAC 3 cut(s) 736, 941, 1146
Hpy188I TCNGA 5 cut(s) 300, 407, 556, 871, 893
Hpy188III TCNNGA 6 cut(s) 157, 278, 389, 391, 605, 998
Hpy8I GTNNAC 3 cut(s) 736, 941, 1146
HpyAV CCTTC 8 cut(s) 218, 310, 509, 553, 752, 886, 1074, 1183
HpyCH4III ACNGT 5 cut(s) 49, 65, 134, 181, 1150
HpyCH4IV ACGT 2 cut(s) 849, 1169
HpyCH4V TGCA 8 cut(s) 104, 293, 308, 565, 686, 969, 976, 1164
HpyF3I CTNAG 5 cut(s) 265, 276, 299, 653, 1189
HpySE526I ACGT 2 cut(s) 849, 1169
Hsp92I GRCGYC 1 cut(s) 849
Hsp92II CATG 6 cut(s) 149, 316, 550, 571, 980, 1054
KroI GCCGGC 1 cut(s) 1194
KroNI GCCGGC 1 cut(s) 1196
Kzo9I GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
LguI GCTCTTC 1 cut(s) 489
Lsp1109I GCAGC 3 cut(s) 88, 551, 974
LweI GCATC 5 cut(s) 280, 317, 340, 442, 673
MabI ACCWGGT 1 cut(s) 960
MaeI CTAG 1 cut(s) 1104
MaeII ACGT 2 cut(s) 849, 1169
MaeIII GTNAC 4 cut(s) 43, 193, 334, 704
MalI GATC 8 cut(s) 244, 255, 381, 446, 660, 863, 873, 954
MboI GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
MflI RGATCY 3 cut(s) 242, 658, 952
MluCI AATT 6 cut(s) 671, 696, 791, 982, 1023, 1111
MlyI GAGTC 1 cut(s) 1117
MmeI TCCRAC 2 cut(s) 1151, 1201
MnlI CCTC 6 cut(s) 529, 746, 863, 1028, 1071, 1150
Mph1103I ATGCAT 2 cut(s) 310, 978
MroNI GCCGGC 1 cut(s) 1194
MseI TTAA 1 cut(s) 957
MslI CAYNNNNRTG 1 cut(s) 144
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 2 cut(s) 234, 1195
MspR9I CCNGG 3 cut(s) 234, 845, 962
MvaI CCWGG 2 cut(s) 845, 962
NaeI GCCGGC 1 cut(s) 1196
NciI CCSGG 1 cut(s) 234
NdeII GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
NgoMIV GCCGGC 1 cut(s) 1194
NlaIII CATG 6 cut(s) 149, 316, 550, 571, 980, 1054
NlaIV GGNNCC 2 cut(s) 520, 660
NmuCI GTSAC 2 cut(s) 334, 704
NsiI ATGCAT 2 cut(s) 310, 978
NspI RCATGY 1 cut(s) 980
PaeI GCATGC 1 cut(s) 980
PaeR7I CTCGAG 1 cut(s) 389
PciSI GCTCTTC 1 cut(s) 489
PcsI WCGNNNNNNNCGW 2 cut(s) 474, 846
PdiI GCCGGC 1 cut(s) 1196
PfeI GAWTC 5 cut(s) 82, 281, 582, 722, 906
PflMI CCANNNNNTGG 1 cut(s) 116
PkrI GCNGC 4 cut(s) 103, 541, 932, 989
PleI GAGTC 1 cut(s) 1117
PpsI GAGTC 1 cut(s) 1117
PsiI TTATAA 1 cut(s) 167
Psp6I CCWGG 2 cut(s) 843, 960
PspGI CCWGG 2 cut(s) 843, 960
PspN4I GGNNCC 2 cut(s) 520, 660
PspPI GGNCC 1 cut(s) 454
PsrI GAACNNNNNNTAC 2 cut(s) 1090, 1122
PstI CTGCAG 1 cut(s) 1166
PsuI RGATCY 3 cut(s) 242, 658, 952
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 4 cut(s) 67, 809, 923, 1168
RsaNI GTAC 4 cut(s) 66, 808, 922, 1167
RseI CAYNNNNRTG 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 1 cut(s) 957
SatI GCNGC 4 cut(s) 102, 540, 931, 988
Sau3AI GATC 8 cut(s) 242, 253, 379, 444, 658, 861, 871, 952
Sau96I GGNCC 1 cut(s) 454
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 1 cut(s) 1117
ScrFI CCNGG 3 cut(s) 234, 845, 962
SexAI ACCWGGT 1 cut(s) 960
SfaNI GCATC 5 cut(s) 280, 317, 340, 442, 673
SfcI CTRYAG 2 cut(s) 96, 1162
Sfr274I CTCGAG 1 cut(s) 389
SinI GGWCC 1 cut(s) 454
SlaI CTCGAG 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 144
SmlI CTYRAG 2 cut(s) 389, 814
SmoI CTYRAG 2 cut(s) 389, 814
SphI GCATGC 1 cut(s) 980
Sse9I AATT 6 cut(s) 671, 696, 791, 982, 1023, 1111
SsiI CCGC 2 cut(s) 237, 930
SspMI CTAG 1 cut(s) 1104
StyD4I CCNGG 3 cut(s) 232, 843, 960
StyI CCWWGG 2 cut(s) 285, 715
TaaI ACNGT 5 cut(s) 49, 65, 134, 181, 1150
TaiI ACGT 2 cut(s) 852, 1172
TaqI TCGA 4 cut(s) 378, 390, 606, 999
TasI AATT 6 cut(s) 671, 696, 791, 982, 1023, 1111
TatI WGTACW 1 cut(s) 921
TauI GCSGC 1 cut(s) 933
TfiI GAWTC 5 cut(s) 82, 281, 582, 722, 906
Tru1I TTAA 1 cut(s) 957
Tru9I TTAA 1 cut(s) 957
TscAI CASTG 6 cut(s) 39, 76, 348, 517, 643, 1062
TseFI GTSAC 2 cut(s) 334, 704
TseI GCWGC 3 cut(s) 101, 539, 987
Tsp45I GTSAC 2 cut(s) 334, 704
TspDTI ATGAA 7 cut(s) 77, 116, 329, 535, 589, 868, 1059
TspGWI ACGGA 2 cut(s) 647, 765
TspRI CASTG 6 cut(s) 39, 76, 348, 517, 643, 1062
Van91I CCANNNNNTGG 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 3 cut(s) 671, 791, 1023
XceI RCATGY 1 cut(s) 980
XhoI CTCGAG 1 cut(s) 389
XspI CTAG 1 cut(s) 1104
ZraI GACGTC 1 cut(s) 850
ZrmI AGTACT 1 cut(s) 923
Zsp2I ATGCAT 2 cut(s) 310, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.