RLG00000005539

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006034
Physical Location & Seq
Forward (+)
68398527 .. 68400984
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005539

Sequence Viewer

Length: 957 bp
ATGATGATGATACAGGATCCCTTGGATGCAGTTCTGAGAAATGCATCCATGAAGGACAAGACCATAATCGTCACAACACTGAATGATGCGTGGGCAGAACCCAACTCAATATTCGATCTGTTTCTCGAGAGTTTTCGGATCGGAAACAACACGAATCGGCTGCTGAAGCATCTGGTGGTGATCTGCTTGGACCAAAAGGCATACGCTCGTTGCTTGGCTCTTCACCCTCATTGCTACCAACTCTACACTCAAGGTGCTAACTTTACCAGCGAGGCAGCTTTCATGTCCTCCGATTACCTGCACATGATGTGGAGAAGAATCCAGTTTTTGACTTCTATTCTCGAAAAAGGATACAGCTTTGTCTTTACGGACACTGACATTATGTGGCTAAGGGACCCATTCCCACAATTCTTCCCGGATGCAGACTTCCAAATTGCATGTGACTATTTCCGAGGCGATTCTTACGATGTGAACAACCTTCCTAATGGAGGATTTACCTATGTAGTCTCTAATAAACGGACCACCCAATTCTACAACTTTTGGTACTTCTCAAGGCAGGCGTATCCGAATAATCACGACCAGGACGTTCTTAACAAGATCAAATCTGACCCGTTCATCACCAGCATCGGATTGAAAATGAGATTCTTGGACACCAAATACTTTGGCGGCTTCTGTCAGCCCAGTAAAGATTTAAACTTGGTTTGCACAATGCACGCTAATTGCTGCGTTGGTCTCGATAACAAAGTCAATGACCTCAAAATTCTGCTTCAAGATTGGAGAAATTACATGGCATTGCCTCCTCCTCCTTCTTCATCTTCTTCCGGTCCTAATTCTAATACCACCACGCCTCCAACAACTATGCCCTCATGGACCGTTCCACAGAACTGCAGTACTTCCTTCCAACGCCAACGCCAACGCCAACGCCAACGCCAACGCCACGGCCGGCTCTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

319

Amino Acids

36.9

Weight (kDa)

8.67

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 54 - 252 4.8e-69 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 306
AciI CCGC 1 cut(s) 666
AclWI GGATC 3 cut(s) 11, 24, 146
AcoI YGGCCR 1 cut(s) 940
AcsI RAATTY 1 cut(s) 759
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 2 cut(s) 545, 892
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 2 cut(s) 634, 770
AjnI CCWGG 1 cut(s) 579
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 2 cut(s) 278, 357
AluI AGCT 2 cut(s) 278, 357
Alw26I GTCTC 2 cut(s) 511, 737
AlwI GGATC 3 cut(s) 11, 24, 146
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 1 cut(s) 940
ApeKI GCWGC 3 cut(s) 160, 275, 723
ApoI RAATTY 1 cut(s) 759
AspS9I GGNCC 5 cut(s) 190, 394, 519, 824, 870
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 3 cut(s) 190, 215, 610
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 5 cut(s) 190, 394, 519, 824, 870
BamHI GGATCC 1 cut(s) 16
BbvI GCAGC 3 cut(s) 147, 287, 710
BceAI ACGGC 1 cut(s) 955
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 581
BciVI GTATCC 2 cut(s) 344, 573
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 2 cut(s) 511, 737
BfmI CTRYAG 1 cut(s) 886
BfuAI ACCTGC 1 cut(s) 306
BfuI GTATCC 2 cut(s) 344, 573
BisI GCNGC 4 cut(s) 161, 276, 667, 724
BlsI GCNGC 4 cut(s) 162, 277, 668, 725
BmcAI AGTACT 1 cut(s) 892
Bme1390I CCNGG 2 cut(s) 416, 581
Bme18I GGWCC 5 cut(s) 190, 394, 519, 824, 870
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 5 cut(s) 190, 394, 519, 824, 870
BmiI GGNNCC 3 cut(s) 18, 395, 396
BmrFI CCNGG 2 cut(s) 416, 581
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 6 cut(s) 16, 53, 76, 178, 409, 633
BmuI ACTGGG 1 cut(s) 675
Bpu10I CCTNAGC 1 cut(s) 389
BpuEI CTTGAG 2 cut(s) 234, 535
BpuMI CCSGG 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 737
BsaJI CCNNGG 3 cut(s) 21, 451, 937
BsaWI WCCGGW 1 cut(s) 821
BsaXI ACNNNNNCTCC 2 cut(s) 832, 862
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 1 cut(s) 942
Bse1I ACTGG 2 cut(s) 322, 681
Bse3DI GCAATG 2 cut(s) 229, 791
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 3 cut(s) 21, 451, 937
BseGI GGATG 3 cut(s) 31, 44, 424
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMI GCAATG 2 cut(s) 229, 791
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 2 cut(s) 322, 681
BseRI GAGGAG 2 cut(s) 789, 792
BseX3I CGGCCG 1 cut(s) 940
BseXI GCAGC 3 cut(s) 147, 287, 710
BsgI GTGCAG 1 cut(s) 284
Bsh1285I CGRYCG 1 cut(s) 943
BshFI GGCC 1 cut(s) 942
BsiEI CGRYCG 1 cut(s) 943
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 3 cut(s) 416, 822, 943
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 2 cut(s) 511, 737
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 1 cut(s) 942
Bso31I GGTCTC 1 cut(s) 737
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 5 cut(s) 16, 115, 138, 180, 597
BspACI CCGC 1 cut(s) 666
BspANI GGCC 1 cut(s) 942
BspCNI CTCAG 1 cut(s) 27
BspLI GGNNCC 3 cut(s) 18, 395, 396
BspMAI CTGCAG 1 cut(s) 890
BspMI ACCTGC 1 cut(s) 306
BspPI GGATC 3 cut(s) 11, 24, 146
BspQI GCTCTTC 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 229, 791
BsrFI RCCGGY 1 cut(s) 942
BsrI ACTGG 2 cut(s) 322, 681
BssAI RCCGGY 1 cut(s) 942
BssECI CCNNGG 3 cut(s) 21, 451, 937
BssMI GATC 5 cut(s) 16, 115, 138, 180, 597
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 1 cut(s) 225
BstC8I GCNNGC 3 cut(s) 558, 714, 944
BstDEI CTNAG 2 cut(s) 35, 389
BstDSI CCRYGG 1 cut(s) 937
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 3 cut(s) 31, 44, 424
BstKTI GATC 5 cut(s) 19, 118, 141, 183, 600
BstMAI GTCTC 2 cut(s) 511, 737
BstMBI GATC 5 cut(s) 16, 115, 138, 180, 597
BstMCI CGRYCG 1 cut(s) 943
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 414, 579
BstSFI CTRYAG 1 cut(s) 886
BstV1I GCAGC 3 cut(s) 147, 287, 710
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BstZI CGGCCG 1 cut(s) 940
BsuI GTATCC 2 cut(s) 344, 573
BsuRI GGCC 1 cut(s) 942
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 3 cut(s) 31, 44, 424
BtsIMutI CAGTG 2 cut(s) 77, 372
BveI ACCTGC 1 cut(s) 306
Cac8I GCNNGC 3 cut(s) 558, 714, 944
Cfr10I RCCGGY 1 cut(s) 942
Cfr13I GGNCC 5 cut(s) 190, 394, 519, 824, 870
Csp6I GTAC 2 cut(s) 544, 891
CviAII CATG 6 cut(s) 49, 283, 304, 438, 787, 867
CviJI RGCY 9 cut(s) 160, 218, 278, 357, 388, 669, 679, 942, 946
CviKI_1 RGCY 9 cut(s) 160, 218, 278, 357, 388, 669, 679, 942, 946
CviQI GTAC 2 cut(s) 544, 891
DdeI CTNAG 2 cut(s) 35, 389
DpnI GATC 5 cut(s) 18, 117, 140, 182, 599
DpnII GATC 5 cut(s) 16, 115, 138, 180, 597
DraI TTTAAA 1 cut(s) 693
EaeI YGGCCR 1 cut(s) 940
EagI CGGCCG 1 cut(s) 940
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
EclXI CGGCCG 1 cut(s) 940
Eco130I CCWWGG 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 737
Eco47I GGWCC 5 cut(s) 190, 394, 519, 824, 870
Eco52I CGGCCG 1 cut(s) 940
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 1 cut(s) 125
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 6 cut(s) 52, 286, 307, 441, 790, 870
FaqI GGGAC 1 cut(s) 407
FatI CATG 6 cut(s) 48, 282, 303, 437, 786, 866
Fnu4HI GCNGC 4 cut(s) 161, 276, 667, 724
FokI GGATG 3 cut(s) 31, 38, 431
Fsp4HI GCNGC 4 cut(s) 161, 276, 667, 724
GluI GCNGC 4 cut(s) 161, 276, 667, 724
HaeIII GGCC 1 cut(s) 942
HapII CCGG 3 cut(s) 416, 822, 943
Hin1II CATG 6 cut(s) 52, 286, 307, 441, 790, 870
HinfI GANTC 4 cut(s) 154, 318, 458, 642
HpaII CCGG 3 cut(s) 416, 822, 943
HphI GGTGA 3 cut(s) 190, 215, 610
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 8 cut(s) 36, 138, 143, 292, 452, 567, 607, 629
Hpy188III TCNNGA 6 cut(s) 125, 127, 341, 575, 734, 770
Hpy8I GTNNAC 1 cut(s) 472
HpyAV CCTTC 4 cut(s) 46, 488, 816, 907
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 8 cut(s) 29, 44, 301, 422, 437, 705, 712, 888
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 2 cut(s) 35, 389
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 6 cut(s) 52, 286, 307, 441, 790, 870
KflI GGGWCCC 1 cut(s) 394
KroI GCCGGC 1 cut(s) 942
KroNI GCCGGC 1 cut(s) 944
Kzo9I GATC 5 cut(s) 16, 115, 138, 180, 597
LguI GCTCTTC 1 cut(s) 225
Lsp1109I GCAGC 3 cut(s) 147, 287, 710
LweI GCATC 6 cut(s) 16, 53, 76, 178, 409, 633
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 2 cut(s) 70, 440
MalI GATC 5 cut(s) 18, 117, 140, 182, 599
MboI GATC 5 cut(s) 16, 115, 138, 180, 597
MboII GAAGA 6 cut(s) 212, 327, 403, 801, 807, 810
MflI RGATCY 1 cut(s) 16
MluCI AATT 7 cut(s) 407, 432, 527, 718, 759, 781, 829
MmeI TCCRAC 2 cut(s) 875, 925
Mph1103I ATGCAT 1 cut(s) 46
MroNI GCCGGC 1 cut(s) 942
MseI TTAA 2 cut(s) 591, 692
MspI CCGG 3 cut(s) 416, 822, 943
MspR9I CCNGG 2 cut(s) 416, 581
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 1 cut(s) 166
NaeI GCCGGC 1 cut(s) 944
NciI CCSGG 1 cut(s) 416
NdeII GATC 5 cut(s) 16, 115, 138, 180, 597
NgoMIV GCCGGC 1 cut(s) 942
NlaIII CATG 6 cut(s) 52, 286, 307, 441, 790, 870
NlaIV GGNNCC 3 cut(s) 18, 395, 396
NmuCI GTSAC 2 cut(s) 70, 440
NsiI ATGCAT 1 cut(s) 46
NspI RCATGY 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 125
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PdiI GCCGGC 1 cut(s) 944
PfeI GAWTC 4 cut(s) 154, 318, 458, 642
PfoI TCCNGGA 1 cut(s) 414
PkrI GCNGC 4 cut(s) 162, 277, 668, 725
PpuMI RGGWCCY 1 cut(s) 394
Psp5II RGGWCCY 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 3 cut(s) 18, 395, 396
PspPI GGNCC 5 cut(s) 190, 394, 519, 824, 870
PspPPI RGGWCCY 1 cut(s) 394
PstI CTGCAG 1 cut(s) 890
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 2 cut(s) 545, 892
RsaNI GTAC 2 cut(s) 544, 891
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 2 cut(s) 591, 692
SatI GCNGC 4 cut(s) 161, 276, 667, 724
Sau3AI GATC 5 cut(s) 16, 115, 138, 180, 597
Sau96I GGNCC 5 cut(s) 190, 394, 519, 824, 870
ScaI AGTACT 1 cut(s) 892
ScrFI CCNGG 2 cut(s) 416, 581
SetI ASST 8 cut(s) 256, 280, 300, 359, 480, 500, 588, 756
SfaNI GCATC 6 cut(s) 16, 53, 76, 178, 409, 633
SfcI CTRYAG 1 cut(s) 886
Sfr274I CTCGAG 1 cut(s) 125
SinI GGWCC 5 cut(s) 190, 394, 519, 824, 870
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 3 cut(s) 125, 249, 550
SmoI CTYRAG 3 cut(s) 125, 249, 550
Sse9I AATT 7 cut(s) 407, 432, 527, 718, 759, 781, 829
SsiI CCGC 1 cut(s) 666
SspI AATATT 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 414, 579
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 1 cut(s) 588
TaqI TCGA 4 cut(s) 114, 126, 342, 735
TasI AATT 7 cut(s) 407, 432, 527, 718, 759, 781, 829
TatI WGTACW 1 cut(s) 890
TauI GCSGC 1 cut(s) 669
TfiI GAWTC 4 cut(s) 154, 318, 458, 642
Tru1I TTAA 2 cut(s) 591, 692
Tru9I TTAA 2 cut(s) 591, 692
TscAI CASTG 2 cut(s) 84, 379
TseFI GTSAC 2 cut(s) 70, 440
TseI GCWGC 3 cut(s) 160, 275, 723
Tsp45I GTSAC 2 cut(s) 70, 440
TspDTI ATGAA 4 cut(s) 65, 271, 604, 801
TspGWI ACGGA 2 cut(s) 383, 532
TspRI CASTG 2 cut(s) 84, 379
VpaK11BI GGWCC 5 cut(s) 190, 394, 519, 824, 870
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 1 cut(s) 759
XceI RCATGY 1 cut(s) 441
XhoI CTCGAG 1 cut(s) 125
ZrmI AGTACT 1 cut(s) 892
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.