pycom06g21330

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006034
Physical Location & Seq
Forward (+)
23993138 .. 23996165
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g21330.1

Sequence Viewer

Length: 1122 bp
ATGAGAAACTCTGTGAATCTTGATATTACTGGGAAGAGGATTAATGCACGGACGATACTGGTGTTTATGGCGGCAGCAGCTGTCGCCGGTTGCTTTATTGTCTACAATTTTGTGTATCCTTCTGGATTTCGTTATGATGGCTTGGCAACACCCCTCCCCTCCTGCAGAATCACAACTCCTCCGCTTCAGCAGGCGAATACCACAACCCCTCCGCTTCAGCAGGCGAATACCACAACCCCTCCACTTCAGCAGGCCAGTACAGGCAACCAACAGGAGGATATGGATCCATTGGATAAAGTTCTGAAAAATGCATGCATGAAGAACAAAACGGTTATAATAACTACGTTAAATGATGCATGGGCAGAGCCCAATTCAATATTTGATCTCTTTCTTGAGAGCTTTAACATCGGAAGCAACACGAAATGGCTGCTCAAGCATTTGCTAGTCATCTGCGTGGACCAAAAAGCACATGCTCGTTGCTTAGCATTACACCCTCATTGTTACTTACTTTACACTCAAGGCGCTAACTTTACCGGCGAGGTATTTTATCTGACTCCCAATTACCTACAGATGATGTGGAGAAGAACCCAGATTTTGTCTTCCATTCTCGACAAAGGCTACAACTTTGTCTTTACGGATACCGATATAATGTGGCTTCGAGATCCATTTCCACAATTCTATCCAGATGCAGATTTTCAAATTGCAACCGATCAATTCCTAGCTGATTCTTATAGTTTTGACAATCCTCCTAATTGTGGATTTGTCAATGTAAAATCCAATGATAGAACTATTGGGTTTTACAAATTTTGGTACCTCTCCAAATACACATATCCAGATACGAATGAACAAGAAGTGCTTAATAGGATTAAATTTGATCCCTTCATTGCCAAGATTGGATTGAAAGTGAGATTCTTGGATACAAAATACTTTGGTGGCTTCTGTGAGCCAAGTAAGGATTTTAAACAAGTTTGCACAATGCATGCTAATTGTTGTGTTGGTCTTGAAAACAAAGTGAACGATCTCAAAGCTTTGCTTCAAGTTTGGAAAAATTACATGTCATCGCCTCGCAACGCCACAGGCACATCACAAGCTTTATGGACCGTCCCACAAAAATGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

374

Amino Acids

42.56

Weight (kDa)

8.5

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 143 - 341 1.4e-62 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 1107
Acc65I GGTACC 1 cut(s) 810
AccB1I GGYRCC 1 cut(s) 810
AccI GTMKAC 1 cut(s) 102
AciI CCGC 3 cut(s) 71, 182, 212
AclWI GGATC 4 cut(s) 278, 291, 656, 869
AcsI RAATTY 2 cut(s) 803, 869
AcuI CTGAAG 3 cut(s) 170, 200, 230
AfaI GTAC 2 cut(s) 259, 812
AfiI CCNNNNNNNGG 2 cut(s) 274, 755
AflIII ACRYGT 1 cut(s) 1053
AgsI TTSAA 5 cut(s) 375, 698, 901, 1004, 1037
AluBI AGCT 5 cut(s) 80, 399, 722, 1028, 1091
AluI AGCT 5 cut(s) 80, 399, 722, 1028, 1091
AlwI GGATC 4 cut(s) 278, 291, 656, 869
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 74, 77, 427
ApoI RAATTY 2 cut(s) 803, 869
AseI ATTAAT 1 cut(s) 42
Asp718I GGTACC 1 cut(s) 810
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 2 cut(s) 457, 1098
AvaII GGWCC 2 cut(s) 457, 1098
BaeI ACNNNNGTAYC 2 cut(s) 47, 80
BamHI GGATCC 1 cut(s) 283
BanI GGYRCC 1 cut(s) 810
BanII GRGCYC 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 86, 89, 414
BccI CCATC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciVI GTATCC 3 cut(s) 126, 631, 910
BfaI CTAG 2 cut(s) 443, 719
BfmI CTRYAG 2 cut(s) 163, 566
BfoI RGCGCY 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 1107
BfuI GTATCC 3 cut(s) 126, 631, 910
BisI GCNGC 4 cut(s) 72, 75, 78, 428
BlpI GCTNAGC 1 cut(s) 481
BlsI GCNGC 4 cut(s) 73, 76, 79, 429
Bme18I GGWCC 2 cut(s) 457, 1098
BmgT120I GGNCC 2 cut(s) 457, 1098
BmiI GGNNCC 2 cut(s) 285, 812
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 2 cut(s) 343, 676
BmuI ACTGGG 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 591
Bpu1102I GCTNAGC 1 cut(s) 481
BpuEI CTTGAG 3 cut(s) 413, 416, 501
BsaBI GATNNNNATC 1 cut(s) 282
BsaXI ACNNNNNCTCC 6 cut(s) 163, 193, 193, 223, 223, 253
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 755
Bse118I RCCGGY 2 cut(s) 86, 533
Bse1I ACTGG 3 cut(s) 34, 63, 255
Bse3DI GCAATG 1 cut(s) 882
Bse8I GATNNNNATC 1 cut(s) 282
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 274, 755
BseMI GCAATG 1 cut(s) 882
BseNI ACTGG 3 cut(s) 34, 63, 255
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 3 cut(s) 86, 89, 414
BshFI GGCC 1 cut(s) 254
BshNI GGYRCC 1 cut(s) 810
BsiSI CCGG 2 cut(s) 87, 534
BslFI GGGAC 1 cut(s) 1088
BslI CCNNNNNNNGG 2 cut(s) 274, 755
BsmFI GGGAC 1 cut(s) 1088
BsnI GGCC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
Bsp1720I GCTNAGC 1 cut(s) 481
BspACI CCGC 3 cut(s) 71, 182, 212
BspANI GGCC 1 cut(s) 254
BspLI GGNNCC 2 cut(s) 285, 812
BspMAI CTGCAG 1 cut(s) 167
BspMI ACCTGC 1 cut(s) 1107
BspPI GGATC 4 cut(s) 278, 291, 656, 869
BspT107I GGYRCC 1 cut(s) 810
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 2 cut(s) 86, 533
BsrI ACTGG 3 cut(s) 34, 63, 255
BssAI RCCGGY 2 cut(s) 86, 533
BssMI GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
Bst4CI ACNGT 2 cut(s) 331, 1102
Bst6I CTCTTC 1 cut(s) 29
BstC8I GCNNGC 5 cut(s) 192, 222, 252, 313, 981
BstDEI CTNAG 1 cut(s) 481
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 6 cut(s) 286, 385, 664, 712, 877, 1021
BstMBI GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
BstMWI GCNNNNNNNGC 3 cut(s) 77, 83, 433
BstNSI RCATGY 4 cut(s) 315, 473, 983, 1057
BstSFI CTRYAG 2 cut(s) 163, 566
BstV1I GCAGC 3 cut(s) 86, 89, 414
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 2 cut(s) 283, 661
BstYI RGATCY 2 cut(s) 283, 661
BsuI GTATCC 3 cut(s) 126, 631, 910
BsuRI GGCC 1 cut(s) 254
BtgZI GCGATG 1 cut(s) 1044
BveI ACCTGC 1 cut(s) 1107
Cac8I GCNNGC 5 cut(s) 192, 222, 252, 313, 981
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 524
Cfr10I RCCGGY 2 cut(s) 86, 533
Cfr13I GGNCC 2 cut(s) 457, 1098
Csp6I GTAC 2 cut(s) 258, 811
CviAII CATG 6 cut(s) 312, 316, 357, 470, 980, 1054
CviQI GTAC 2 cut(s) 258, 811
DdeI CTNAG 1 cut(s) 481
DpnI GATC 6 cut(s) 285, 384, 663, 711, 876, 1020
DpnII GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
DraI TTTAAA 1 cut(s) 961
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco24I GRGCYC 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 457, 1098
Eco57I CTGAAG 3 cut(s) 170, 200, 230
EcoT22I ATGCAT 4 cut(s) 313, 317, 358, 981
EcoT38I GRGCYC 1 cut(s) 369
FaeI CATG 6 cut(s) 315, 319, 360, 473, 983, 1057
FalI AAGNNNNNCTT 4 cut(s) 840, 872, 1017, 1049
FaqI GGGAC 1 cut(s) 1088
FatI CATG 6 cut(s) 311, 315, 356, 469, 979, 1053
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 4 cut(s) 72, 75, 78, 428
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 4 cut(s) 72, 75, 78, 428
FspBI CTAG 2 cut(s) 443, 719
GlaI GCGC 1 cut(s) 523
GluI GCNGC 4 cut(s) 72, 75, 78, 428
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 1 cut(s) 254
HapII CCGG 2 cut(s) 87, 534
HhaI GCGC 1 cut(s) 524
Hin1II CATG 6 cut(s) 315, 319, 360, 473, 983, 1057
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HindIII AAGCTT 2 cut(s) 1026, 1089
HinfI GANTC 5 cut(s) 16, 168, 553, 725, 909
HpaII CCGG 2 cut(s) 87, 534
Hpy166II GTNNAC 3 cut(s) 103, 457, 1015
Hpy188I TCNGA 3 cut(s) 303, 410, 552
Hpy188III TCNNGA 8 cut(s) 20, 123, 392, 608, 659, 683, 833, 1001
Hpy8I GTNNAC 3 cut(s) 103, 457, 1015
HpyAV CCTTC 2 cut(s) 129, 889
HpyCH4III ACNGT 2 cut(s) 331, 1102
HpyCH4IV ACGT 1 cut(s) 344
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 83, 433
HpyF3I CTNAG 1 cut(s) 481
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 6 cut(s) 315, 319, 360, 473, 983, 1057
HspAI GCGC 1 cut(s) 522
KpnI GGTACC 1 cut(s) 814
Kzo9I GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
Lsp1109I GCAGC 3 cut(s) 86, 89, 414
LweI GCATC 2 cut(s) 343, 676
MaeI CTAG 2 cut(s) 443, 719
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 500
MalI GATC 6 cut(s) 285, 384, 663, 711, 876, 1020
MboI GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
MboII GAAGA 4 cut(s) 46, 331, 591, 594
MflI RGATCY 2 cut(s) 283, 661
MhlI GDGCHC 1 cut(s) 369
MlyI GAGTC 1 cut(s) 547
Mph1103I ATGCAT 4 cut(s) 313, 317, 358, 981
MseI TTAA 6 cut(s) 42, 347, 402, 858, 867, 960
MslI CAYNNNNRTG 2 cut(s) 452, 1111
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 2 cut(s) 87, 534
MwoI GCNNNNNNNGC 3 cut(s) 77, 83, 433
NdeII GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
NlaIII CATG 6 cut(s) 315, 319, 360, 473, 983, 1057
NlaIV GGNNCC 2 cut(s) 285, 812
NsiI ATGCAT 4 cut(s) 313, 317, 358, 981
NspI RCATGY 4 cut(s) 315, 473, 983, 1057
PaeI GCATGC 2 cut(s) 315, 983
PciI ACATGT 1 cut(s) 1053
PfeI GAWTC 4 cut(s) 16, 168, 725, 909
PkrI GCNGC 4 cut(s) 73, 76, 79, 429
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PscI ACATGT 1 cut(s) 1053
PshBI ATTAAT 1 cut(s) 42
PsiI TTATAA 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 285, 812
PspPI GGNCC 2 cut(s) 457, 1098
PstI CTGCAG 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 2 cut(s) 283, 661
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 2 cut(s) 259, 812
RsaNI GTAC 2 cut(s) 258, 811
RseI CAYNNNNRTG 2 cut(s) 452, 1111
SaqAI TTAA 6 cut(s) 42, 347, 402, 858, 867, 960
SatI GCNGC 4 cut(s) 72, 75, 78, 428
Sau3AI GATC 6 cut(s) 283, 382, 661, 709, 874, 1018
Sau96I GGNCC 2 cut(s) 457, 1098
SchI GAGTC 1 cut(s) 547
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 2 cut(s) 343, 676
SfcI CTRYAG 2 cut(s) 163, 566
SinI GGWCC 2 cut(s) 457, 1098
SmiMI CAYNNNNRTG 2 cut(s) 452, 1111
SmlI CTYRAG 3 cut(s) 392, 431, 516
SmoI CTYRAG 3 cut(s) 392, 431, 516
SphI GCATGC 2 cut(s) 315, 983
SsiI CCGC 3 cut(s) 71, 182, 212
SspI AATATT 1 cut(s) 378
SspMI CTAG 2 cut(s) 443, 719
TaaI ACNGT 2 cut(s) 331, 1102
TaiI ACGT 1 cut(s) 347
TaqI TCGA 2 cut(s) 609, 658
TatI WGTACW 1 cut(s) 257
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 4 cut(s) 16, 168, 725, 909
Tru1I TTAA 6 cut(s) 42, 347, 402, 858, 867, 960
Tru9I TTAA 6 cut(s) 42, 347, 402, 858, 867, 960
TseI GCWGC 3 cut(s) 74, 77, 427
TspDTI ATGAA 3 cut(s) 332, 858, 871
TspGWI ACGGA 2 cut(s) 64, 650
VpaK11BI GGWCC 2 cut(s) 457, 1098
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 2 cut(s) 803, 869
XceI RCATGY 4 cut(s) 315, 473, 983, 1057
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 2 cut(s) 443, 719
Zsp2I ATGCAT 4 cut(s) 313, 317, 358, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.