Rw7G000440

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006034
Physical Location & Seq
Reverse (-)
366760 .. 369626
2867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000440.1

Sequence Viewer

Length: 1203 bp
ATGAGCAGTAAATCATTATCACCAGTAGCAGCACTATTATCTGTGACAGTTATTGGGAATAACAGTACTGGTGTTATGGAGATTAACACAACAGCTGCAACACCAAAGGCTGGTGGTGTTCATCACCATTATCATCATCATCATCATCATCACGGATTGGTCAAGAGGATTCTAAATATTGCTCTGGTGTGTGTGGCTGTTACTCTGGCATCCCTTCTCTTCTACATCGCTGTCTTCCCCGGCGGCGGACCTATTTATGATCCTTTGTTCTCAGCTAAATCTCAGGATCCCTTGGATGCAGTTCTGAGAAATGCATCCATGAAGGACAAGACCATAATCGTCACAACACTAAATGATGCGTGGGCAGAACCCAACTCAATATTCGATCTGTTTCTCGAGAGTTTTCGGATCGGAAACAACACGAATCGGCTGCTGAAGCATCTGGTGGTGATCTGCTTGGACCAAAAGGCATACTCTCGTTGCTTGGCTCTTCACCCTCACTGCTACCAACTCTACACTCAAGGTGCCAACTTTACCAGCGAGGCAGCTTTCATGTCCTCCGATTACTTGCACATGATGTGGAGAAGAATCCAGTTTTTGACTTCTATTCTCGAAAAAGGATACAGCTTTGTCTTTACGGATACTGACATTATGTGGCTTAGAGACCCATTCCCACAATTCTTCCCAGATGCAGACTTCCAAATTGCATGTGACTACTTCCGTGGCGATTCTTACGATGTGAACAACCTTCCGAACGGAGGATTTACCTATGTAGTCTCTAATAAACGGACCACCCAATTCTACAACTTTTGGTACTTCTCAAGGCAGGCGTATCCGAATAATCACGACCAGGACGTGCTCAACAAGATCAAATCTGATCCGTTCATCACCAGCATCGGATTGAAAATGAGATTCTTGGACACCAAATACTTTGGCGGCTTCTGTCAGCCCAGTAAAGATTTAAACCTGGTTTGCACAATGCACGCTAATTGCTGTGTTGGTCTCGATAACAAAGTCAATGACCTCAAAATTCTGCTTCAAGATTGGAGAAATTACGTGGCATTGCCTCCTTCTCCTTCTTCATCTTCTTCCGGTCCTACTTCTAATGCCACCACGCCCCCAACAACTATGCCCTCATGGACCGTTCCACAGAACTGCAGTACTTCCTTCCAACGCCAACGCCACGGCCGGCACTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

400

Amino Acids

45.1

Weight (kDa)

8.45

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 144 - 342 7.1e-69 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 524
AccB7I CCANNNNNTGG 1 cut(s) 110
AciI CCGC 3 cut(s) 243, 246, 936
AclWI GGATC 5 cut(s) 254, 281, 294, 416, 872
AcoI YGGCCR 1 cut(s) 1186
AcsI RAATTY 1 cut(s) 1029
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 3 cut(s) 67, 815, 1162
AfiI CCNNNNNNNGG 3 cut(s) 110, 245, 758
AgsI TTSAA 2 cut(s) 904, 1040
AjiI CACGTC 1 cut(s) 856
AjnI CCWGG 2 cut(s) 849, 966
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 4 cut(s) 95, 275, 548, 627
AluI AGCT 4 cut(s) 95, 275, 548, 627
Alw21I GWGCWC 1 cut(s) 861
Alw26I GTCTC 3 cut(s) 657, 781, 1007
AlwI GGATC 5 cut(s) 254, 281, 294, 416, 872
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 1 cut(s) 1186
ApeKI GCWGC 4 cut(s) 29, 95, 430, 545
ApoI RAATTY 1 cut(s) 1029
AspS9I GGNCC 5 cut(s) 248, 460, 789, 1094, 1140
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 5 cut(s) 12, 116, 460, 485, 880
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 5 cut(s) 248, 460, 789, 1094, 1140
BamHI GGATCC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 861
BbvI GCAGC 4 cut(s) 41, 82, 417, 557
BceAI ACGGC 1 cut(s) 1201
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BciT130I CCWGG 2 cut(s) 851, 968
BciVI GTATCC 3 cut(s) 614, 634, 843
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 3 cut(s) 657, 781, 1007
BfmI CTRYAG 1 cut(s) 1156
BfuI GTATCC 3 cut(s) 614, 634, 843
BisI GCNGC 6 cut(s) 30, 96, 244, 431, 546, 937
BlsI GCNGC 6 cut(s) 31, 97, 245, 432, 547, 938
BmcAI AGTACT 2 cut(s) 67, 1162
Bme1390I CCNGG 3 cut(s) 240, 851, 968
Bme18I GGWCC 5 cut(s) 248, 460, 789, 1094, 1140
BmeT110I CYCGRG 1 cut(s) 395
BmgBI CACGTC 1 cut(s) 856
BmgT120I GGNCC 5 cut(s) 248, 460, 789, 1094, 1140
BmiI GGNNCC 2 cut(s) 288, 526
BmrFI CCNGG 3 cut(s) 240, 851, 968
BmrI ACTGGG 1 cut(s) 945
BmsI GCATC 7 cut(s) 218, 286, 323, 346, 448, 679, 903
BmuI ACTGGG 1 cut(s) 945
BpiI GAAGAC 1 cut(s) 226
BpuEI CTTGAG 2 cut(s) 504, 805
BpuMI CCSGG 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 1057
BsaI GGTCTC 2 cut(s) 657, 1007
BsaJI CCNNGG 4 cut(s) 238, 291, 721, 1183
BsaWI WCCGGW 1 cut(s) 1091
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 245, 758
Bse118I RCCGGY 1 cut(s) 1188
Bse1I ACTGG 4 cut(s) 23, 73, 592, 951
Bse3DI GCAATG 1 cut(s) 1061
BseBI CCWGG 2 cut(s) 851, 968
BseDI CCNNGG 4 cut(s) 238, 291, 721, 1183
BseGI GGATG 3 cut(s) 209, 301, 314
BseLI CCNNNNNNNGG 3 cut(s) 110, 245, 758
BseMI GCAATG 1 cut(s) 1061
BseMII CTCAG 3 cut(s) 285, 296, 296
BseNI ACTGG 4 cut(s) 23, 73, 592, 951
BseX3I CGGCCG 1 cut(s) 1186
BseXI GCAGC 4 cut(s) 41, 82, 417, 557
Bsh1285I CGRYCG 1 cut(s) 1189
BshFI GGCC 1 cut(s) 1188
BshNI GGYRCC 1 cut(s) 524
BsiEI CGRYCG 1 cut(s) 1189
BsiHKAI GWGCWC 1 cut(s) 861
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 3 cut(s) 240, 1092, 1189
BslI CCNNNNNNNGG 3 cut(s) 110, 245, 758
BsmAI GTCTC 3 cut(s) 657, 781, 1007
BsnI GGCC 1 cut(s) 1188
Bso31I GGTCTC 2 cut(s) 657, 1007
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 861
Bsp143I GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
BspACI CCGC 3 cut(s) 243, 246, 936
BspANI GGCC 1 cut(s) 1188
BspCNI CTCAG 3 cut(s) 284, 295, 297
BspLI GGNNCC 2 cut(s) 288, 526
BspMAI CTGCAG 1 cut(s) 1160
BspPI GGATC 5 cut(s) 254, 281, 294, 416, 872
BspQI GCTCTTC 1 cut(s) 495
BspT107I GGYRCC 1 cut(s) 524
BspTNI GGTCTC 2 cut(s) 657, 1007
BsrDI GCAATG 1 cut(s) 1061
BsrFI RCCGGY 1 cut(s) 1188
BsrI ACTGG 4 cut(s) 23, 73, 592, 951
BssAI RCCGGY 1 cut(s) 1188
BssECI CCNNGG 4 cut(s) 238, 291, 721, 1183
BssMI GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 851, 968
Bst4CI ACNGT 3 cut(s) 49, 65, 1144
Bst6I CTCTTC 2 cut(s) 224, 495
BstBAI YACGTR 1 cut(s) 1057
BstC8I GCNNGC 3 cut(s) 828, 984, 1190
BstDEI CTNAG 4 cut(s) 271, 282, 305, 659
BstDSI CCRYGG 2 cut(s) 721, 1183
BstF5I GGATG 3 cut(s) 209, 301, 314
BstKTI GATC 7 cut(s) 262, 289, 388, 411, 453, 870, 880
BstMAI GTCTC 3 cut(s) 657, 781, 1007
BstMBI GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
BstMCI CGRYCG 1 cut(s) 1189
BstMWI GCNNNNNNNGC 1 cut(s) 436
BstNI CCWGG 2 cut(s) 851, 968
BstNSI RCATGY 1 cut(s) 711
BstSCI CCNGG 3 cut(s) 238, 849, 966
BstSFI CTRYAG 1 cut(s) 1156
BstV1I GCAGC 4 cut(s) 41, 82, 417, 557
BstV2I GAAGAC 1 cut(s) 226
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BstZI CGGCCG 1 cut(s) 1186
BsuI GTATCC 3 cut(s) 614, 634, 843
BsuRI GGCC 1 cut(s) 1188
BtgI CCRYGG 2 cut(s) 721, 1183
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 856
BtsCI GGATG 3 cut(s) 209, 301, 314
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 3 cut(s) 828, 984, 1190
Cfr10I RCCGGY 1 cut(s) 1188
Cfr13I GGNCC 5 cut(s) 248, 460, 789, 1094, 1140
CsiI ACCWGGT 1 cut(s) 966
Csp6I GTAC 3 cut(s) 66, 814, 1161
CviAII CATG 5 cut(s) 319, 553, 574, 708, 1137
CviQI GTAC 3 cut(s) 66, 814, 1161
DdeI CTNAG 4 cut(s) 271, 282, 305, 659
DpnI GATC 7 cut(s) 261, 288, 387, 410, 452, 869, 879
DpnII GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
DraI TTTAAA 1 cut(s) 963
EaeI YGGCCR 1 cut(s) 1186
EagI CGGCCG 1 cut(s) 1186
Eam1104I CTCTTC 2 cut(s) 224, 495
EarI CTCTTC 2 cut(s) 224, 495
EciI GGCGGA 1 cut(s) 261
EclXI CGGCCG 1 cut(s) 1186
Eco130I CCWWGG 1 cut(s) 291
Eco31I GGTCTC 2 cut(s) 657, 1007
Eco47I GGWCC 5 cut(s) 248, 460, 789, 1094, 1140
Eco52I CGGCCG 1 cut(s) 1186
Eco57I CTGAAG 1 cut(s) 455
Eco88I CYCGRG 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 849, 966
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 5 cut(s) 322, 556, 577, 711, 1140
FatI CATG 5 cut(s) 318, 552, 573, 707, 1136
Fnu4HI GCNGC 6 cut(s) 30, 96, 244, 431, 546, 937
FokI GGATG 3 cut(s) 196, 301, 308
Fsp4HI GCNGC 6 cut(s) 30, 96, 244, 431, 546, 937
GluI GCNGC 6 cut(s) 30, 96, 244, 431, 546, 937
HaeIII GGCC 1 cut(s) 1188
HapII CCGG 3 cut(s) 240, 1092, 1189
Hin1II CATG 5 cut(s) 322, 556, 577, 711, 1140
HinfI GANTC 5 cut(s) 169, 424, 588, 728, 912
HpaII CCGG 3 cut(s) 240, 1092, 1189
HphI GGTGA 5 cut(s) 12, 116, 460, 485, 880
Hpy166II GTNNAC 1 cut(s) 742
Hpy188I TCNGA 8 cut(s) 306, 408, 413, 562, 753, 837, 877, 899
Hpy188III TCNNGA 8 cut(s) 163, 284, 395, 397, 611, 845, 1004, 1040
Hpy8I GTNNAC 1 cut(s) 742
HpyAV CCTTC 6 cut(s) 224, 316, 758, 1080, 1086, 1177
HpyCH4III ACNGT 3 cut(s) 49, 65, 1144
HpyCH4IV ACGT 2 cut(s) 855, 1056
HpyCH4V TGCA 9 cut(s) 98, 299, 314, 571, 692, 707, 975, 982, 1158
HpyF10VI GCNNNNNNNGC 1 cut(s) 436
HpyF3I CTNAG 4 cut(s) 271, 282, 305, 659
HpySE526I ACGT 2 cut(s) 855, 1056
Hsp92II CATG 5 cut(s) 322, 556, 577, 711, 1140
KroI GCCGGC 1 cut(s) 1188
KroNI GCCGGC 1 cut(s) 1190
Kzo9I GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
LguI GCTCTTC 1 cut(s) 495
Lsp1109I GCAGC 4 cut(s) 41, 82, 417, 557
LweI GCATC 7 cut(s) 218, 286, 323, 346, 448, 679, 903
MabI ACCWGGT 1 cut(s) 966
MaeII ACGT 2 cut(s) 855, 1056
MaeIII GTNAC 4 cut(s) 43, 199, 340, 710
MalI GATC 7 cut(s) 261, 288, 387, 410, 452, 869, 879
MboI GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
MboII GAAGA 8 cut(s) 211, 226, 482, 597, 673, 1071, 1077, 1080
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 861
MluCI AATT 6 cut(s) 677, 702, 797, 988, 1029, 1051
MmeI TCCRAC 1 cut(s) 1195
MnlI CCTC 8 cut(s) 159, 507, 535, 568, 752, 1034, 1077, 1144
Mph1103I ATGCAT 1 cut(s) 316
MroNI GCCGGC 1 cut(s) 1188
MseI TTAA 2 cut(s) 84, 962
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 3 cut(s) 240, 1092, 1189
MspR9I CCNGG 3 cut(s) 240, 851, 968
MvaI CCWGG 2 cut(s) 851, 968
MwoI GCNNNNNNNGC 1 cut(s) 436
NaeI GCCGGC 1 cut(s) 1190
NciI CCSGG 1 cut(s) 240
NdeII GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
NgoMIV GCCGGC 1 cut(s) 1188
NlaIII CATG 5 cut(s) 322, 556, 577, 711, 1140
NlaIV GGNNCC 2 cut(s) 288, 526
NmuCI GTSAC 3 cut(s) 43, 340, 710
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 1 cut(s) 711
PaeR7I CTCGAG 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 852
PdiI GCCGGC 1 cut(s) 1190
PfeI GAWTC 5 cut(s) 169, 424, 588, 728, 912
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 6 cut(s) 31, 97, 245, 432, 547, 938
Ppu21I YACGTR 1 cut(s) 1057
Psp6I CCWGG 2 cut(s) 849, 966
PspGI CCWGG 2 cut(s) 849, 966
PspN4I GGNNCC 2 cut(s) 288, 526
PspPI GGNCC 5 cut(s) 248, 460, 789, 1094, 1140
PstI CTGCAG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 3 cut(s) 67, 815, 1162
RsaNI GTAC 3 cut(s) 66, 814, 1161
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 2 cut(s) 84, 962
SatI GCNGC 6 cut(s) 30, 96, 244, 431, 546, 937
Sau3AI GATC 7 cut(s) 259, 286, 385, 408, 450, 867, 877
Sau96I GGNCC 5 cut(s) 248, 460, 789, 1094, 1140
ScaI AGTACT 2 cut(s) 67, 1162
ScrFI CCNGG 3 cut(s) 240, 851, 968
SduI GDGCHC 1 cut(s) 861
SexAI ACCWGGT 1 cut(s) 966
SfaNI GCATC 7 cut(s) 218, 286, 323, 346, 448, 679, 903
SfcI CTRYAG 1 cut(s) 1156
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 5 cut(s) 248, 460, 789, 1094, 1140
SlaI CTCGAG 1 cut(s) 395
SmlI CTYRAG 3 cut(s) 395, 519, 820
SmoI CTYRAG 3 cut(s) 395, 519, 820
Sse9I AATT 6 cut(s) 677, 702, 797, 988, 1029, 1051
SsiI CCGC 3 cut(s) 243, 246, 936
SspI AATATT 2 cut(s) 178, 381
StyD4I CCNGG 3 cut(s) 238, 849, 966
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 3 cut(s) 49, 65, 1144
TaiI ACGT 2 cut(s) 858, 1059
TaqI TCGA 4 cut(s) 384, 396, 612, 1005
TasI AATT 6 cut(s) 677, 702, 797, 988, 1029, 1051
TatI WGTACW 2 cut(s) 65, 1160
TauI GCSGC 2 cut(s) 246, 939
TfiI GAWTC 5 cut(s) 169, 424, 588, 728, 912
Tru1I TTAA 2 cut(s) 84, 962
Tru9I TTAA 2 cut(s) 84, 962
TscAI CASTG 1 cut(s) 506
TseFI GTSAC 3 cut(s) 43, 340, 710
TseI GCWGC 4 cut(s) 29, 95, 430, 545
Tsp45I GTSAC 3 cut(s) 43, 340, 710
TspDTI ATGAA 5 cut(s) 110, 335, 541, 874, 1071
TspGWI ACGGA 6 cut(s) 168, 653, 710, 771, 802, 870
TspRI CASTG 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 5 cut(s) 248, 460, 789, 1094, 1140
XapI RAATTY 1 cut(s) 1029
XceI RCATGY 1 cut(s) 711
XhoI CTCGAG 1 cut(s) 395
ZrmI AGTACT 2 cut(s) 67, 1162
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.