MD06G1237600.v1.1

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006034
Physical Location & Seq
Forward (+)
36877463 .. 36882039
4577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1237600.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGAGAAACTCTGTGATTCTTGATATTACTGGGAAGAGGATTACTGCACGGACGATACTGGTGTTTATGGCGGCAGCAGCTGTCGCCGGTTGCTTTATTGTCTACAATTTTGTGTATCCTTCTGGATTTCGTTATGATGGCTTGGCATCACCCCTCCCCTCCTGCAGAACCACAACCCCTCCACTTCAGCAGGCGAATACCACAACTCCTCCGCTTCAGCAGGCGAATACCACAACCCCTTCGCTTCAGCAGGCGAATACCACAACTCCTCCGCTTCAGCAGGCAAATACCACAACCCCTTCGCTTCAGCAGGGCAACCAACAAGAGGATATGGATCCATTGGATAAAGTTCTGAAAAATGCATGCATGAAGAACAAAACGGTTATAATAACTACGTTAAATGATGCATGGGCAGAGCCCAATTCAATATTTGATCTCTTTCTTGAGAGCTTTAACATCGGAAGCAACACGAAATGGCTGCTCAAGCATTTGCTAGTCATCTGCGTGGACCAAAAAGCATATGCTCGTTGCTTAGCATCACACCCTCATTGTTACTTACTTTACACTCAAGGCACCAACTTTACCGGCGAGGTATTTTATCTGACTCCCAATTACCTACGGATGATGTGGAGAAGAACCCAGATTTTGTCTTCCATTCTCGACAAAGGCTACAACTTTATCTTTACGGATACCGATATAATGTGGCTTCGAGATCCATTTCCACAATTCTATCCGGATGCAGATTTTCAAATTGCAACTGATCAATTCCTAGCTGATTCTTATAGTTTTGACAATCCTCCCAACTGTGGATTTGTCAATGTAAAATCCAATGATCGAACTATTGGGTTTTACAAATTTTGGTACCTCTCCAAATACACATATCCAGATACGAATGAACAAGAAGTGCTTAATAGGATTAAATTTGATCCCTTCATTGCCAAGATTGGATTGAAAGTGAGATTCTTGGATACAAAATACTTTGGTGGCTTCTGTGAGCCAAGTAAGGATTTTAACCAAGTTTGCACAATGCATGCCAATTGTTGTGTTGGTCTTGAAAACAAAGTGAACGATCTCAAAGCTTTGCTTCAAGTTTGGAAAAATTACATGTCATCACCTCGTAACGCCACAGGCACATCACAAGCTTTATGGACCGTCCCACAAAAATGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

391

Amino Acids

44.41

Weight (kDa)

8.5

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 160 - 358 8e-63 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 1158
Acc65I GGTACC 1 cut(s) 861
AccB1I GGYRCC 2 cut(s) 572, 861
AccI GTMKAC 1 cut(s) 102
AccIII TCCGGA 1 cut(s) 733
AciI CCGC 3 cut(s) 71, 212, 272
AclWI GGATC 4 cut(s) 329, 342, 707, 920
AcsI RAATTY 2 cut(s) 854, 920
AcuI CTGAAG 5 cut(s) 170, 200, 230, 260, 290
AfaI GTAC 1 cut(s) 863
AfiI CCNNNNNNNGG 2 cut(s) 325, 806
AflIII ACRYGT 1 cut(s) 1104
AgsI TTSAA 5 cut(s) 426, 749, 952, 1055, 1088
AluBI AGCT 5 cut(s) 80, 450, 773, 1079, 1142
AluI AGCT 5 cut(s) 80, 450, 773, 1079, 1142
AlwI GGATC 4 cut(s) 329, 342, 707, 920
AlwNI CAGNNNCTG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 733
ApeKI GCWGC 3 cut(s) 74, 77, 478
ApoI RAATTY 2 cut(s) 854, 920
Asp718I GGTACC 1 cut(s) 861
AspS9I GGNCC 2 cut(s) 508, 1149
AsuHPI GGTGA 2 cut(s) 141, 1104
AvaII GGWCC 2 cut(s) 508, 1149
BaeI ACNNNNGTAYC 2 cut(s) 47, 80
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 2 cut(s) 572, 861
BanII GRGCYC 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 642
BbvI GCAGC 3 cut(s) 86, 89, 465
BccI CCATC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciVI GTATCC 3 cut(s) 126, 682, 961
BclI TGATCA 1 cut(s) 760
BfaI CTAG 2 cut(s) 494, 770
BfmI CTRYAG 1 cut(s) 163
BfuAI ACCTGC 1 cut(s) 1158
BfuI GTATCC 3 cut(s) 126, 682, 961
BisI GCNGC 4 cut(s) 72, 75, 78, 479
BlpI GCTNAGC 1 cut(s) 532
BlsI GCNGC 4 cut(s) 73, 76, 79, 480
Bme18I GGWCC 2 cut(s) 508, 1149
BmgT120I GGNCC 2 cut(s) 508, 1149
BmiI GGNNCC 3 cut(s) 336, 574, 863
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 4 cut(s) 155, 394, 545, 727
BmuI ACTGGG 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 642
Bpu1102I GCTNAGC 1 cut(s) 532
BpuEI CTTGAG 3 cut(s) 464, 467, 552
BsaBI GATNNNNATC 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 733
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 806
Bse118I RCCGGY 2 cut(s) 86, 584
Bse1I ACTGG 2 cut(s) 34, 63
Bse3DI GCAATG 1 cut(s) 933
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 733
BseGI GGATG 2 cut(s) 627, 742
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 325, 806
BseMI GCAATG 1 cut(s) 933
BseNI ACTGG 2 cut(s) 34, 63
BseRI GAGGAG 2 cut(s) 198, 258
BseXI GCAGC 3 cut(s) 86, 89, 465
BsgI GTGCAG 1 cut(s) 30
BshNI GGYRCC 2 cut(s) 572, 861
BsiSI CCGG 3 cut(s) 87, 585, 734
BslFI GGGAC 1 cut(s) 1139
BslI CCNNNNNNNGG 2 cut(s) 325, 806
BsmFI GGGAC 1 cut(s) 1139
Bsp1286I GDGCHC 1 cut(s) 420
Bsp13I TCCGGA 1 cut(s) 733
Bsp143I GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
Bsp1720I GCTNAGC 1 cut(s) 532
BspACI CCGC 3 cut(s) 71, 212, 272
BspEI TCCGGA 1 cut(s) 733
BspLI GGNNCC 3 cut(s) 336, 574, 863
BspMAI CTGCAG 1 cut(s) 167
BspMI ACCTGC 1 cut(s) 1158
BspPI GGATC 4 cut(s) 329, 342, 707, 920
BspT107I GGYRCC 2 cut(s) 572, 861
BsrDI GCAATG 1 cut(s) 933
BsrFI RCCGGY 2 cut(s) 86, 584
BsrI ACTGG 2 cut(s) 34, 63
BssAI RCCGGY 2 cut(s) 86, 584
BssMI GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
Bst4CI ACNGT 3 cut(s) 382, 806, 1153
Bst6I CTCTTC 1 cut(s) 29
BstC8I GCNNGC 6 cut(s) 192, 222, 252, 282, 364, 1032
BstDEI CTNAG 1 cut(s) 532
BstF5I GGATG 2 cut(s) 627, 742
BstKTI GATC 7 cut(s) 337, 436, 715, 763, 835, 928, 1072
BstMBI GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 77, 83, 484
BstNSI RCATGY 3 cut(s) 366, 1034, 1108
BstSFI CTRYAG 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 86, 89, 465
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 2 cut(s) 334, 712
BstYI RGATCY 2 cut(s) 334, 712
BsuI GTATCC 3 cut(s) 126, 682, 961
BtsCI GGATG 2 cut(s) 627, 742
BveI ACCTGC 1 cut(s) 1158
Cac8I GCNNGC 6 cut(s) 192, 222, 252, 282, 364, 1032
CaiI CAGNNNCTG 1 cut(s) 80
Cfr10I RCCGGY 2 cut(s) 86, 584
Cfr13I GGNCC 2 cut(s) 508, 1149
Csp6I GTAC 1 cut(s) 862
CviAII CATG 5 cut(s) 363, 367, 408, 1031, 1105
CviQI GTAC 1 cut(s) 862
DdeI CTNAG 1 cut(s) 532
DpnI GATC 7 cut(s) 336, 435, 714, 762, 834, 927, 1071
DpnII GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco24I GRGCYC 1 cut(s) 420
Eco47I GGWCC 2 cut(s) 508, 1149
Eco57I CTGAAG 5 cut(s) 170, 200, 230, 260, 290
EcoT22I ATGCAT 4 cut(s) 364, 368, 409, 1032
EcoT38I GRGCYC 1 cut(s) 420
FaeI CATG 5 cut(s) 366, 370, 411, 1034, 1108
FalI AAGNNNNNCTT 4 cut(s) 891, 923, 1068, 1100
FaqI GGGAC 1 cut(s) 1139
FatI CATG 5 cut(s) 362, 366, 407, 1030, 1104
FauNDI CATATG 1 cut(s) 520
FbaI TGATCA 1 cut(s) 760
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 4 cut(s) 72, 75, 78, 479
FokI GGATG 2 cut(s) 634, 749
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 4 cut(s) 72, 75, 78, 479
FspBI CTAG 2 cut(s) 494, 770
GluI GCNGC 4 cut(s) 72, 75, 78, 479
HapII CCGG 3 cut(s) 87, 585, 734
Hin1II CATG 5 cut(s) 366, 370, 411, 1034, 1108
HindIII AAGCTT 2 cut(s) 1077, 1140
HinfI GANTC 4 cut(s) 16, 604, 776, 960
HpaII CCGG 3 cut(s) 87, 585, 734
HphI GGTGA 2 cut(s) 141, 1104
Hpy166II GTNNAC 3 cut(s) 103, 508, 1066
Hpy188I TCNGA 3 cut(s) 354, 461, 603
Hpy188III TCNNGA 8 cut(s) 20, 123, 443, 659, 710, 734, 884, 1052
Hpy8I GTNNAC 3 cut(s) 103, 508, 1066
HpyAV CCTTC 4 cut(s) 129, 249, 309, 940
HpyCH4III ACNGT 3 cut(s) 382, 806, 1153
HpyCH4IV ACGT 1 cut(s) 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 83, 484
HpyF3I CTNAG 1 cut(s) 532
HpySE526I ACGT 1 cut(s) 395
Hsp92II CATG 5 cut(s) 366, 370, 411, 1034, 1108
Kpn2I TCCGGA 1 cut(s) 733
KpnI GGTACC 1 cut(s) 865
Ksp22I TGATCA 1 cut(s) 760
Kzo9I GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
Lsp1109I GCAGC 3 cut(s) 86, 89, 465
LweI GCATC 4 cut(s) 155, 394, 545, 727
MaeI CTAG 2 cut(s) 494, 770
MaeII ACGT 1 cut(s) 395
MaeIII GTNAC 2 cut(s) 551, 1118
MalI GATC 7 cut(s) 336, 435, 714, 762, 834, 927, 1071
MboI GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
MboII GAAGA 4 cut(s) 46, 382, 642, 645
MfeI CAATTG 1 cut(s) 1036
MflI RGATCY 2 cut(s) 334, 712
MhlI GDGCHC 1 cut(s) 420
MlyI GAGTC 1 cut(s) 598
Mph1103I ATGCAT 4 cut(s) 364, 368, 409, 1032
MroI TCCGGA 1 cut(s) 733
MseI TTAA 5 cut(s) 398, 453, 909, 918, 1011
MslI CAYNNNNRTG 2 cut(s) 503, 1162
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 3 cut(s) 87, 585, 734
MunI CAATTG 1 cut(s) 1036
MwoI GCNNNNNNNGC 3 cut(s) 77, 83, 484
NdeI CATATG 1 cut(s) 520
NdeII GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
NlaIII CATG 5 cut(s) 366, 370, 411, 1034, 1108
NlaIV GGNNCC 3 cut(s) 336, 574, 863
NsiI ATGCAT 4 cut(s) 364, 368, 409, 1032
NspI RCATGY 3 cut(s) 366, 1034, 1108
PaeI GCATGC 2 cut(s) 366, 1034
PciI ACATGT 1 cut(s) 1104
PfeI GAWTC 3 cut(s) 16, 776, 960
PkrI GCNGC 4 cut(s) 73, 76, 79, 480
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
PscI ACATGT 1 cut(s) 1104
PsiI TTATAA 1 cut(s) 386
PspN4I GGNNCC 3 cut(s) 336, 574, 863
PspPI GGNCC 2 cut(s) 508, 1149
PstI CTGCAG 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 2 cut(s) 334, 712
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 2 cut(s) 503, 1162
SaqAI TTAA 5 cut(s) 398, 453, 909, 918, 1011
SatI GCNGC 4 cut(s) 72, 75, 78, 479
Sau3AI GATC 7 cut(s) 334, 433, 712, 760, 832, 925, 1069
Sau96I GGNCC 2 cut(s) 508, 1149
SchI GAGTC 1 cut(s) 598
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 4 cut(s) 155, 394, 545, 727
SfcI CTRYAG 1 cut(s) 163
SinI GGWCC 2 cut(s) 508, 1149
SmiMI CAYNNNNRTG 2 cut(s) 503, 1162
SmlI CTYRAG 3 cut(s) 443, 482, 567
SmoI CTYRAG 3 cut(s) 443, 482, 567
SphI GCATGC 2 cut(s) 366, 1034
SsiI CCGC 3 cut(s) 71, 212, 272
SspI AATATT 1 cut(s) 429
SspMI CTAG 2 cut(s) 494, 770
TaaI ACNGT 3 cut(s) 382, 806, 1153
TaiI ACGT 1 cut(s) 398
TaqI TCGA 3 cut(s) 660, 709, 835
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 3 cut(s) 16, 776, 960
Tru1I TTAA 5 cut(s) 398, 453, 909, 918, 1011
Tru9I TTAA 5 cut(s) 398, 453, 909, 918, 1011
TseI GCWGC 3 cut(s) 74, 77, 478
TspDTI ATGAA 3 cut(s) 383, 909, 922
TspGWI ACGGA 3 cut(s) 64, 634, 701
VpaK11BI GGWCC 2 cut(s) 508, 1149
XapI RAATTY 2 cut(s) 854, 920
XceI RCATGY 3 cut(s) 366, 1034, 1108
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 2 cut(s) 494, 770
Zsp2I ATGCAT 4 cut(s) 364, 368, 409, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.