MD14G1244100.v1.1

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006034
Physical Location & Seq
Forward (+)
32245728 .. 32248695
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1244100.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGAGCAAATTATCGGCAGATGAAGCAGCTGGAAATTATGTTAGCGGGAATAATATGATGAAATTACTAGTATCATCATCATCATTATCATCATCATCGTCATCACCATCATCCAACGGTGGAAACGGATCATCAGGTAGCAGTAATCGAGCGTTGGTGGCGAAGAAGTTTCTAAAGGTGACGTTGCTGTTTGCGGCAGCCACTGTGTGTTGCTTTGGCTTCTACAATTCTGTCTACACTTCCGGATTTCTGCTCAGATCCTCTTATGACTACGCTTTGTCTGGCACCAGAACCGTAACTTCTCATCAGACCAAAGTAGAAGATCTATTGGATACAGTTCTTAAAAATGCATCTATGAAGAACAAAACCATTATCGTGACTACTCTAAATGATGCATGGGCAGAACCCAATTCAATATTCGATTCATTTTTAGAGAGTTTCGAGATCGGAAGTAACACAAAATGGCTGCTCAAGCATTTGGTGGTCATATGCTTGGACAAAAAAGCATATGCGCGTTGCTTGGCATCACACCCTCATTGCTACCAACTCTACACTCAAGGTGCCAACTTCACCAGCGAAGCTGCTTTCATGTCTTCAAAGTACCTGGACATGATGTGGAGACGAATCCAATTTTTGTCTTCTATTCTCGACAGAGGATACAACTTTGTCTTTACAGACACTGATATAATGTGGCTACGAAATCCATTCCCACGATTCTATCCAGATGCAGATTTTCAAATTGCATGCGATTACTTCATTGGTGATTCTTATAGCATGAAGAACTTTCCAAATGGAGGGTTTACTTATGTAAAATCAAACAATCGCACGATTCAATTTTATAAGTTTTGGTACTTCTCAAGAAAGGCGTATCCAAAGATGCATGACCAAGACGTTCTCAATGAGATCAAATTTGATCCCTTCCTCACCCAAATTGGATTGAAAATGAGGTTCTTGGATACACTATACTTTGGTGGCTTCTGCCAGGCGAGTAAGGACTTTAACCAAGTTTGCACAATGCATGCCAATTGTTGTGTTGGCCTCGAAAACAAAGTCAATGATCTTAAAATTTTGCTTAAAGTTTGGAGAAAGTACATGTCCTTGCCTCCTAATGCAACTGCCACACCACAGCTTTCATGGACTGTTCCACAAAATTGCAGCACTTCCTTCCAACGCCTTGAAAAGCACTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

397

Amino Acids

44.87

Weight (kDa)

9.02

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 156 - 354 5.2e-72 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AccB1I GGYRCC 2 cut(s) 284, 560
AccI GTMKAC 1 cut(s) 234
AccII CGCG 1 cut(s) 514
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 2 cut(s) 45, 194
AclWI GGATC 3 cut(s) 136, 252, 908
AcsI RAATTY 2 cut(s) 908, 1065
AdeI CACNNNGTG 1 cut(s) 207
AfaI GTAC 3 cut(s) 602, 851, 1091
AfiI CCNNNNNNNGG 1 cut(s) 794
AflIII ACRYGT 1 cut(s) 1092
AgsI TTSAA 6 cut(s) 414, 597, 737, 833, 940, 1178
AhlI ACTAGT 1 cut(s) 67
AjnI CCWGG 2 cut(s) 603, 981
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AluBI AGCT 3 cut(s) 29, 581, 1129
AluI AGCT 3 cut(s) 29, 581, 1129
Alw26I GTCTC 1 cut(s) 613
AlwI GGATC 3 cut(s) 136, 252, 908
AlwNI CAGNNNCTG 2 cut(s) 203, 680
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 1 cut(s) 1036
ApeKI GCWGC 5 cut(s) 26, 197, 466, 581, 1155
ApoI RAATTY 2 cut(s) 908, 1065
ArsI GACNNNNNNTTYG 2 cut(s) 1035, 1067
AspLEI GCGC 1 cut(s) 514
AsuHPI GGTGA 5 cut(s) 96, 190, 562, 773, 916
BanI GGYRCC 2 cut(s) 284, 560
BauI CACGAG 1 cut(s) 1186
BbsI GAAGAC 2 cut(s) 585, 630
BbvI GCAGC 5 cut(s) 38, 209, 453, 568, 1167
BccI CCATC 1 cut(s) 115
BciT130I CCWGG 2 cut(s) 605, 983
BciVI GTATCC 4 cut(s) 325, 650, 879, 949
BcoDI GTCTC 1 cut(s) 613
BcuI ACTAGT 1 cut(s) 67
BfaI CTAG 1 cut(s) 68
BfuI GTATCC 4 cut(s) 325, 650, 879, 949
BglII AGATCT 1 cut(s) 322
BisI GCNGC 6 cut(s) 27, 195, 198, 467, 582, 1156
BlsI GCNGC 6 cut(s) 28, 196, 199, 468, 583, 1157
Bme1390I CCNGG 2 cut(s) 605, 983
BmiI GGNNCC 2 cut(s) 286, 562
BmrFI CCNGG 2 cut(s) 605, 983
BmsI GCATC 5 cut(s) 359, 382, 533, 715, 867
BpiI GAAGAC 2 cut(s) 585, 630
BpuEI CTTGAG 3 cut(s) 455, 540, 841
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 1 cut(s) 794
Bse3DI GCAATG 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 2 cut(s) 605, 983
BseGI GGATG 1 cut(s) 110
BseLI CCNNNNNNNGG 1 cut(s) 794
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 268
BseXI GCAGC 5 cut(s) 38, 209, 453, 568, 1167
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 1 cut(s) 1038
BshNI GGYRCC 2 cut(s) 284, 560
BsiSI CCGG 1 cut(s) 243
BslI CCNNNNNNNGG 1 cut(s) 794
BsmAI GTCTC 1 cut(s) 613
BsmBI CGTCTC 1 cut(s) 613
BsnI GGCC 1 cut(s) 1038
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
BspACI CCGC 2 cut(s) 45, 194
BspANI GGCC 1 cut(s) 1038
BspCNI CTCAG 1 cut(s) 267
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 286, 562
BspPI GGATC 3 cut(s) 136, 252, 908
BspT107I GGYRCC 2 cut(s) 284, 560
BsrDI GCAATG 1 cut(s) 535
BssMI GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
BssSI CACGAG 1 cut(s) 1186
Bst2BI CACGAG 1 cut(s) 1186
Bst2UI CCWGG 2 cut(s) 605, 983
Bst4CI ACNGT 5 cut(s) 119, 205, 295, 337, 1141
BstC8I GCNNGC 2 cut(s) 745, 1020
BstDEI CTNAG 1 cut(s) 254
BstF5I GGATG 1 cut(s) 110
BstFNI CGCG 1 cut(s) 514
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 7 cut(s) 131, 260, 325, 447, 906, 916, 1060
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
BstMWI GCNNNNNNNGC 3 cut(s) 23, 158, 472
BstNI CCWGG 2 cut(s) 605, 983
BstNSI RCATGY 3 cut(s) 747, 1022, 1096
BstSCI CCNGG 2 cut(s) 603, 981
BstUI CGCG 1 cut(s) 514
BstV1I GCAGC 5 cut(s) 38, 209, 453, 568, 1167
BstV2I GAAGAC 2 cut(s) 585, 630
BstX2I RGATCY 2 cut(s) 257, 322
BstYI RGATCY 2 cut(s) 257, 322
BsuI GTATCC 4 cut(s) 325, 650, 879, 949
BsuRI GGCC 1 cut(s) 1038
BtsCI GGATG 1 cut(s) 110
BtsIMutI CAGTG 2 cut(s) 201, 678
Cac8I GCNNGC 2 cut(s) 745, 1020
CaiI CAGNNNCTG 2 cut(s) 203, 680
CfoI GCGC 1 cut(s) 514
Csp6I GTAC 3 cut(s) 601, 850, 1090
CviAII CATG 9 cut(s) 396, 589, 610, 744, 775, 881, 1019, 1093, 1134
CviJI RGCY 9 cut(s) 29, 200, 219, 466, 581, 694, 975, 1038, 1129
CviKI_1 RGCY 9 cut(s) 29, 200, 219, 466, 581, 694, 975, 1038, 1129
CviQI GTAC 3 cut(s) 601, 850, 1090
DdeI CTNAG 1 cut(s) 254
DpnI GATC 7 cut(s) 130, 259, 324, 446, 905, 915, 1059
DpnII GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
DraIII CACNNNGTG 1 cut(s) 207
EcoRII CCWGG 2 cut(s) 603, 981
EcoT22I ATGCAT 4 cut(s) 352, 397, 882, 1020
Esp3I CGTCTC 1 cut(s) 613
FaeI CATG 9 cut(s) 399, 592, 613, 747, 778, 884, 1022, 1096, 1137
FatI CATG 9 cut(s) 395, 588, 609, 743, 774, 880, 1018, 1092, 1133
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 2 cut(s) 488, 508
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 6 cut(s) 27, 195, 198, 467, 582, 1156
FokI GGATG 1 cut(s) 97
Fsp4HI GCNGC 6 cut(s) 27, 195, 198, 467, 582, 1156
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 513
GluI GCNGC 6 cut(s) 27, 195, 198, 467, 582, 1156
HaeIII GGCC 1 cut(s) 1038
HapII CCGG 1 cut(s) 243
HhaI GCGC 1 cut(s) 514
Hin1II CATG 9 cut(s) 399, 592, 613, 747, 778, 884, 1022, 1096, 1137
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HinfI GANTC 5 cut(s) 422, 624, 714, 764, 829
HpaII CCGG 1 cut(s) 243
HphI GGTGA 5 cut(s) 96, 190, 562, 773, 916
Hpy166II GTNNAC 2 cut(s) 235, 801
Hpy188I TCNGA 3 cut(s) 257, 309, 449
Hpy188III TCNNGA 7 cut(s) 243, 376, 442, 647, 722, 858, 1188
Hpy8I GTNNAC 2 cut(s) 235, 801
HpyAV CCTTC 2 cut(s) 928, 1174
HpyCH4III ACNGT 5 cut(s) 119, 205, 295, 337, 1141
HpyCH4IV ACGT 2 cut(s) 182, 891
HpyCH4V TGCA 9 cut(s) 350, 395, 728, 743, 880, 1011, 1018, 1112, 1155
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 158, 472
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 2 cut(s) 182, 891
Hsp92II CATG 9 cut(s) 399, 592, 613, 747, 778, 884, 1022, 1096, 1137
HspAI GCGC 1 cut(s) 512
Kpn2I TCCGGA 1 cut(s) 242
Kzo9I GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
Lsp1109I GCAGC 5 cut(s) 38, 209, 453, 568, 1167
LweI GCATC 5 cut(s) 359, 382, 533, 715, 867
MaeI CTAG 1 cut(s) 68
MaeII ACGT 2 cut(s) 182, 891
MaeIII GTNAC 4 cut(s) 178, 295, 376, 452
MalI GATC 7 cut(s) 130, 259, 324, 446, 905, 915, 1059
MboI GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
MboII GAAGA 6 cut(s) 175, 332, 370, 585, 630, 790
MfeI CAATTG 1 cut(s) 1024
MflI RGATCY 2 cut(s) 257, 322
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 8 cut(s) 271, 543, 647, 788, 932, 939, 1049, 1113
Mph1103I ATGCAT 4 cut(s) 352, 397, 882, 1020
MroI TCCGGA 1 cut(s) 242
MseI TTAA 4 cut(s) 342, 999, 1062, 1074
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 605, 983
MunI CAATTG 1 cut(s) 1024
MvaI CCWGG 2 cut(s) 605, 983
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 3 cut(s) 23, 158, 472
NdeI CATATG 2 cut(s) 488, 508
NdeII GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
NlaIII CATG 9 cut(s) 399, 592, 613, 747, 778, 884, 1022, 1096, 1137
NlaIV GGNNCC 2 cut(s) 286, 562
NmuCI GTSAC 2 cut(s) 178, 376
NsiI ATGCAT 4 cut(s) 352, 397, 882, 1020
NspI RCATGY 3 cut(s) 747, 1022, 1096
PaeI GCATGC 2 cut(s) 747, 1022
PciI ACATGT 1 cut(s) 1092
PfeI GAWTC 5 cut(s) 422, 624, 714, 764, 829
PkrI GCNGC 6 cut(s) 28, 196, 199, 468, 583, 1157
PscI ACATGT 1 cut(s) 1092
PsiI TTATAA 1 cut(s) 840
Psp6I CCWGG 2 cut(s) 603, 981
PspGI CCWGG 2 cut(s) 603, 981
PspN4I GGNNCC 2 cut(s) 286, 562
PstNI CAGNNNCTG 2 cut(s) 203, 680
PsuI RGATCY 2 cut(s) 257, 322
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 3 cut(s) 602, 851, 1091
RsaNI GTAC 3 cut(s) 601, 850, 1090
RseI CAYNNNNRTG 1 cut(s) 374
SaqAI TTAA 4 cut(s) 342, 999, 1062, 1074
SatI GCNGC 6 cut(s) 27, 195, 198, 467, 582, 1156
Sau3AI GATC 7 cut(s) 128, 257, 322, 444, 903, 913, 1057
ScrFI CCNGG 2 cut(s) 605, 983
SfaNI GCATC 5 cut(s) 359, 382, 533, 715, 867
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 3 cut(s) 470, 555, 856
SmoI CTYRAG 3 cut(s) 470, 555, 856
SpeI ACTAGT 1 cut(s) 67
SphI GCATGC 2 cut(s) 747, 1022
SsiI CCGC 2 cut(s) 45, 194
SspI AATATT 1 cut(s) 417
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 2 cut(s) 603, 981
TaaI ACNGT 5 cut(s) 119, 205, 295, 337, 1141
TaiI ACGT 2 cut(s) 185, 894
TaqI TCGA 5 cut(s) 148, 420, 441, 648, 1041
TatI WGTACW 1 cut(s) 1089
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 5 cut(s) 422, 624, 714, 764, 829
Tru1I TTAA 4 cut(s) 342, 999, 1062, 1074
Tru9I TTAA 4 cut(s) 342, 999, 1062, 1074
TscAI CASTG 2 cut(s) 208, 685
TseFI GTSAC 2 cut(s) 178, 376
TseI GCWGC 5 cut(s) 26, 197, 466, 581, 1155
Tsp45I GTSAC 2 cut(s) 178, 376
TspDTI ATGAA 8 cut(s) 36, 74, 371, 414, 577, 745, 791, 1122
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 208, 685
XapI RAATTY 2 cut(s) 908, 1065
XceI RCATGY 3 cut(s) 747, 1022, 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 1131
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 1 cut(s) 68
Zsp2I ATGCAT 4 cut(s) 352, 397, 882, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.