MD06G1237300.v1.1

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006034
Physical Location & Seq
Forward (+)
36853593 .. 36857379
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1237300.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGAGCAAATTATCGGCAGATGAAGCAGCTGGAAATTATGTTAGCGAGAATGATATGATGAAATTGCCAATATCATCATCACCATCGTCACCATCATCCAACGGTGGAAAAGGATCATCAGGCTGCAGTAACCGAGCGTTGGTGGTGAAGAGGATTCTAAGGGCGACGTTGCTGTTTGTGGCAGCCACTGTGGGTTGCTTTATCATCTCCGGATTTCTGCTCGGATCCTATTATGACAACGGTTTGTCTTCTCATCAGACCACTCTAGAATATACATTGGATACAGTTCTCAGAAATGCATCTATGAAGGACAAAACAATTATACTAACTACCCTAAATGATGCATGGGCTGAACCCAATTCAATATTCGATCTCTTTTTAGAGAGTTTCGAGATTGGAAGTAACACAAAATGGTTGCTTAAGCATTTGGTAGTCATATGCTTGGACAGAAAAGCATATGCCCGTTGCTTGGCGTCACACCCTCATTGCTACCAACTCTACACTCAAGGTGCCAACTTCACCAGCGAGGCTACTTTTATGTCTTCCGAGTACCTGGACATGATGTGGAGACGAATCCGACTTTTGTCTTCTATTCTCGACAGCGGTTACAACTTTGTCTTTACGGATACTGATATAATGTGGCTAAGAGATCCATTCCCACAATTCTACTCAAATGCAGATTTTCAAATTGCATGTGACTTCTTCAACGGTGATTCTTACAGCGTAAATAATTTTCCCAATGGAGGGTTTACCTATGTAAAATCAAACCACCGCACGATTAAATTTTATAAGTTTTGGTACTTCTCCAGAAAGGCGTATCCAAAGAAGCATGACCAAGACGTTCTCAACAAGATCAAATTTGATCGCTTCCTCACCCAAATTGGATTGAAAATGAGGTTCTTGGATACACTATACTTTGGTGGGTTCTGCCAGGCTAGTAAGGACTTCAACCAAGTATGCACAATGCATGCCAATTGTTGTGTTGGTCTCGAAAACAAAGTCAATGATCTTAAAATTTTGCTACAAGTTTGGAGAAAGTACATGTCCTTACCTCCTAATACAACTGCCAAGCCACAACTTTCATGGACCGTTCCACAAAATTGCAGCACTTCCTTCCAACGCCTTGGAAAGCACTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

380

Amino Acids

43.16

Weight (kDa)

8.91

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 139 - 337 8.8e-69 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 509
AccIII TCCGGA 1 cut(s) 209
AciI CCGC 2 cut(s) 603, 772
AclWI GGATC 4 cut(s) 121, 219, 232, 644
AcsI RAATTY 3 cut(s) 782, 857, 1014
AcyI GRCGYC 1 cut(s) 473
AfaI GTAC 3 cut(s) 551, 800, 1040
AfiI CCNNNNNNNGG 4 cut(s) 139, 469, 743, 744
AflII CTTAAG 1 cut(s) 419
AflIII ACRYGT 1 cut(s) 1041
AgsI TTSAA 5 cut(s) 363, 686, 706, 889, 949
AjnI CCWGG 2 cut(s) 552, 930
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 1 cut(s) 29
AluI AGCT 1 cut(s) 29
Alw26I GTCTC 2 cut(s) 562, 992
AlwI GGATC 4 cut(s) 121, 219, 232, 644
AlwNI CAGNNNCTG 1 cut(s) 188
Aor13HI TCCGGA 1 cut(s) 209
ApeKI GCWGC 4 cut(s) 26, 123, 182, 1104
ApoI RAATTY 3 cut(s) 782, 857, 1014
ArsI GACNNNNNNTTYG 2 cut(s) 984, 1016
AspS9I GGNCC 1 cut(s) 1086
AsuHPI GGTGA 6 cut(s) 72, 81, 157, 511, 722, 865
AvaII GGWCC 1 cut(s) 1086
BamHI GGATCC 1 cut(s) 224
BanI GGYRCC 1 cut(s) 509
BauI CACGAG 1 cut(s) 1135
BbsI GAAGAC 3 cut(s) 240, 534, 579
BbvI GCAGC 4 cut(s) 38, 110, 194, 1116
BccI CCATC 2 cut(s) 91, 100
BciT130I CCWGG 2 cut(s) 554, 932
BciVI GTATCC 4 cut(s) 274, 619, 828, 898
BcoDI GTCTC 2 cut(s) 562, 992
BfaI CTAG 2 cut(s) 266, 936
BfmI CTRYAG 1 cut(s) 124
BfrI CTTAAG 1 cut(s) 419
BfuI GTATCC 4 cut(s) 274, 619, 828, 898
BisI GCNGC 4 cut(s) 27, 124, 183, 1105
BlsI GCNGC 4 cut(s) 28, 125, 184, 1106
Bme1390I CCNGG 2 cut(s) 554, 932
Bme18I GGWCC 1 cut(s) 1086
BmgT120I GGNCC 1 cut(s) 1086
BmiI GGNNCC 2 cut(s) 226, 511
BmrFI CCNGG 2 cut(s) 554, 932
BmsI GCATC 2 cut(s) 308, 331
BoxI GACNNNNGTC 1 cut(s) 583
BpiI GAAGAC 3 cut(s) 240, 534, 579
BpmI CTGGAG 1 cut(s) 790
BpuEI CTTGAG 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 992
BsaJI CCNNGG 1 cut(s) 1123
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 469, 743, 744
Bse3DI GCAATG 1 cut(s) 484
BseAI TCCGGA 1 cut(s) 209
BseBI CCWGG 2 cut(s) 554, 932
BseDI CCNNGG 1 cut(s) 1123
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 4 cut(s) 139, 469, 743, 744
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 1 cut(s) 304
BseXI GCAGC 4 cut(s) 38, 110, 194, 1116
BshNI GGYRCC 1 cut(s) 509
BsiSI CCGG 1 cut(s) 210
BslI CCNNNNNNNGG 4 cut(s) 139, 469, 743, 744
BsmAI GTCTC 2 cut(s) 562, 992
BsmBI CGTCTC 1 cut(s) 562
Bso31I GGTCTC 1 cut(s) 992
Bsp13I TCCGGA 1 cut(s) 209
Bsp143I GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
BspACI CCGC 2 cut(s) 603, 772
BspCNI CTCAG 1 cut(s) 303
BspEI TCCGGA 1 cut(s) 209
BspLI GGNNCC 2 cut(s) 226, 511
BspMAI CTGCAG 1 cut(s) 128
BspPI GGATC 4 cut(s) 121, 219, 232, 644
BspT107I GGYRCC 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 992
BsrDI GCAATG 1 cut(s) 484
BssECI CCNNGG 1 cut(s) 1123
BssMI GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
BssNI GRCGYC 1 cut(s) 473
BssSI CACGAG 1 cut(s) 1135
BssT1I CCWWGG 1 cut(s) 1123
Bst2BI CACGAG 1 cut(s) 1135
Bst2UI CCWGG 2 cut(s) 554, 932
Bst4CI ACNGT 6 cut(s) 104, 190, 242, 286, 710, 1090
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 1 cut(s) 473
BstAFI CTTAAG 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 969
BstDEI CTNAG 3 cut(s) 158, 290, 644
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 7 cut(s) 116, 227, 373, 652, 855, 865, 1009
BstMAI GTCTC 2 cut(s) 562, 992
BstMBI GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 554, 932
BstNSI RCATGY 3 cut(s) 696, 971, 1045
BstPAI GACNNNNGTC 1 cut(s) 583
BstSCI CCNGG 2 cut(s) 552, 930
BstSFI CTRYAG 1 cut(s) 124
BstV1I GCAGC 4 cut(s) 38, 110, 194, 1116
BstV2I GAAGAC 3 cut(s) 240, 534, 579
BstX2I RGATCY 2 cut(s) 224, 649
BstXI CCANNNNNNTGG 1 cut(s) 1124
BstYI RGATCY 2 cut(s) 224, 649
BsuI GTATCC 4 cut(s) 274, 619, 828, 898
BtsCI GGATG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 969
CaiI CAGNNNCTG 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 1086
CseI GACGC 1 cut(s) 462
Csp6I GTAC 3 cut(s) 550, 799, 1039
CviAII CATG 7 cut(s) 345, 559, 693, 830, 968, 1042, 1083
CviJI RGCY 8 cut(s) 29, 123, 185, 350, 530, 643, 935, 1072
CviKI_1 RGCY 8 cut(s) 29, 123, 185, 350, 530, 643, 935, 1072
CviQI GTAC 3 cut(s) 550, 799, 1039
DdeI CTNAG 3 cut(s) 158, 290, 644
DpnI GATC 7 cut(s) 115, 226, 372, 651, 854, 864, 1008
DpnII GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 1123
Eco31I GGTCTC 1 cut(s) 992
Eco47I GGWCC 1 cut(s) 1086
EcoRII CCWGG 2 cut(s) 552, 930
EcoT14I CCWWGG 1 cut(s) 1123
EcoT22I ATGCAT 3 cut(s) 301, 346, 969
ErhI CCWWGG 1 cut(s) 1123
Esp3I CGTCTC 1 cut(s) 562
FaeI CATG 7 cut(s) 348, 562, 696, 833, 971, 1045, 1086
FatI CATG 7 cut(s) 344, 558, 692, 829, 967, 1041, 1082
FauNDI CATATG 2 cut(s) 437, 457
Fnu4HI GCNGC 4 cut(s) 27, 124, 183, 1105
FokI GGATG 1 cut(s) 82
Fsp4HI GCNGC 4 cut(s) 27, 124, 183, 1105
FspBI CTAG 2 cut(s) 266, 936
GluI GCNGC 4 cut(s) 27, 124, 183, 1105
GsuI CTGGAG 1 cut(s) 790
HapII CCGG 1 cut(s) 210
HgaI GACGC 1 cut(s) 462
Hin1I GRCGYC 1 cut(s) 473
Hin1II CATG 7 cut(s) 348, 562, 696, 833, 971, 1045, 1086
HinfI GANTC 3 cut(s) 154, 573, 713
HpaII CCGG 1 cut(s) 210
HphI GGTGA 6 cut(s) 72, 81, 157, 511, 722, 865
Hpy166II GTNNAC 1 cut(s) 750
Hpy188I TCNGA 5 cut(s) 224, 258, 293, 547, 578
Hpy188III TCNNGA 7 cut(s) 210, 266, 391, 596, 807, 989, 1137
Hpy8I GTNNAC 1 cut(s) 750
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 2 cut(s) 301, 1123
HpyCH4III ACNGT 6 cut(s) 104, 190, 242, 286, 710, 1090
HpyCH4IV ACGT 2 cut(s) 167, 840
HpyCH4V TGCA 8 cut(s) 126, 299, 344, 677, 692, 960, 967, 1104
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 3 cut(s) 158, 290, 644
HpySE526I ACGT 2 cut(s) 167, 840
Hsp92I GRCGYC 1 cut(s) 473
Hsp92II CATG 7 cut(s) 348, 562, 696, 833, 971, 1045, 1086
Kpn2I TCCGGA 1 cut(s) 209
Kzo9I GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
LpnPI CCDG 9 cut(s) 15, 105, 223, 535, 539, 566, 820, 917, 944
Lsp1109I GCAGC 4 cut(s) 38, 110, 194, 1116
LweI GCATC 2 cut(s) 308, 331
MaeI CTAG 2 cut(s) 266, 936
MaeII ACGT 2 cut(s) 167, 840
MaeIII GTNAC 6 cut(s) 87, 128, 401, 474, 605, 695
MalI GATC 7 cut(s) 115, 226, 372, 651, 854, 864, 1008
MboI GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
MboII GAAGA 5 cut(s) 160, 240, 534, 579, 694
MfeI CAATTG 1 cut(s) 973
MflI RGATCY 2 cut(s) 224, 649
MmeI TCCRAC 2 cut(s) 123, 601
MnlI CCTC 7 cut(s) 144, 492, 520, 737, 881, 888, 1062
Mph1103I ATGCAT 3 cut(s) 301, 346, 969
MroI TCCGGA 1 cut(s) 209
MseI TTAA 3 cut(s) 420, 780, 1011
MspA1I CMGCKG 2 cut(s) 29, 603
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 554, 932
MunI CAATTG 1 cut(s) 973
MvaI CCWGG 2 cut(s) 554, 932
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeI CATATG 2 cut(s) 437, 457
NdeII GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
NlaIII CATG 7 cut(s) 348, 562, 696, 833, 971, 1045, 1086
NlaIV GGNNCC 2 cut(s) 226, 511
NmuCI GTSAC 3 cut(s) 87, 474, 695
NsiI ATGCAT 3 cut(s) 301, 346, 969
NspI RCATGY 3 cut(s) 696, 971, 1045
PaeI GCATGC 1 cut(s) 971
PciI ACATGT 1 cut(s) 1041
PfeI GAWTC 3 cut(s) 154, 573, 713
PkrI GCNGC 4 cut(s) 28, 125, 184, 1106
PscI ACATGT 1 cut(s) 1041
PshAI GACNNNNGTC 1 cut(s) 583
PsiI TTATAA 1 cut(s) 789
Psp6I CCWGG 2 cut(s) 552, 930
PspGI CCWGG 2 cut(s) 552, 930
PspN4I GGNNCC 2 cut(s) 226, 511
PspPI GGNCC 1 cut(s) 1086
PstI CTGCAG 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 224, 649
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 3 cut(s) 551, 800, 1040
RsaNI GTAC 3 cut(s) 550, 799, 1039
SaqAI TTAA 3 cut(s) 420, 780, 1011
SatI GCNGC 4 cut(s) 27, 124, 183, 1105
Sau3AI GATC 7 cut(s) 113, 224, 370, 649, 852, 862, 1006
Sau96I GGNCC 1 cut(s) 1086
ScrFI CCNGG 2 cut(s) 554, 932
SetI ASST 8 cut(s) 31, 170, 511, 555, 755, 843, 899, 1054
SfaNI GCATC 2 cut(s) 308, 331
SfcI CTRYAG 1 cut(s) 124
SinI GGWCC 1 cut(s) 1086
SmlI CTYRAG 2 cut(s) 419, 504
SmoI CTYRAG 2 cut(s) 419, 504
SphI GCATGC 1 cut(s) 971
SsiI CCGC 2 cut(s) 603, 772
SspI AATATT 1 cut(s) 366
SspMI CTAG 2 cut(s) 266, 936
StyD4I CCNGG 2 cut(s) 552, 930
StyI CCWWGG 1 cut(s) 1123
TaaI ACNGT 6 cut(s) 104, 190, 242, 286, 710, 1090
TaiI ACGT 2 cut(s) 170, 843
TaqI TCGA 4 cut(s) 369, 390, 597, 990
TatI WGTACW 1 cut(s) 1038
TfiI GAWTC 3 cut(s) 154, 573, 713
Tru1I TTAA 3 cut(s) 420, 780, 1011
Tru9I TTAA 3 cut(s) 420, 780, 1011
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 3 cut(s) 87, 474, 695
TseI GCWGC 4 cut(s) 26, 123, 182, 1104
Tsp45I GTSAC 3 cut(s) 87, 474, 695
TspDTI ATGAA 4 cut(s) 36, 74, 320, 1071
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 1 cut(s) 193
Vha464I CTTAAG 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 1086
XapI RAATTY 3 cut(s) 782, 857, 1014
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 3 cut(s) 696, 971, 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 1080
XspI CTAG 2 cut(s) 266, 936
Zsp2I ATGCAT 3 cut(s) 301, 346, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.