MD06G1237400.v1.1

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006034
Physical Location & Seq
Forward (+)
36861284 .. 36864216
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1237400.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGAGAAACTGTCGCCAGTTTGGCATATATATATATAGGAAGATGACTAATGTACGGACGACGCTGGTGTTTATGGCGGCAGCAGCTGTCGCCGGTTGCTTTATCGCCTACAATTTTGTGTATCCTCCTGGATTTCTTTATGATGGCTTGGCACCACGCCTCCCCTCCTGCAGAAACACAATCCCTCCACCTCAGCAGGGCAACCAACTGGAGGATAATATGGATCCATTGGATAAAATTCTGAAAAATGCATGCATGAAGAACAAAACAGTTATAATAACTACGTTAAATGATGCATGGGCAGAACCCAATTCAATATTTGATCTCTTTCTTGAAAGCTTTAACATCGGAAGTAACACGAAATGGCTGCTCAAGCATTTGCTAGTCATCTGCGTGGACCAAACAGCATATGCTCGTTGTTTAGTGATACACCCTCATTGTTACCTACTTTATACTCAAGGTGCCAACTTTACCGGCGAGGTATTTTATCTAACCCCCGATTACTTACAAATGATGTGGAGAAGAACCCAGATTTTGTCTTCCATTCTCGACAAAGGCTACAACTTTATCTTTACGGATACCGATATAATGTGGCTTCGAGATCCATTCCCACAATTCTATCCCGATGCAGATTTTCAAATTGCAGCTGATAAATTCATGGGTGATTCTTATAGTTTTAAAAATGCTCCCAATTGTGGATTTGTCAATGTAAAATCCAATGATCGGACTATTGGGTTTTACAAATTTTGGTACCTCTCCAAATACACGTATCCAAAAACGAATGAACAAGAAGTGCTTAATAGGATTAAATTTGATCCTTTCATTGGCAAGATTGGATTGAAAGTGAGATTCTTGGATACAAAATACTTTGGTGGATTCTGTGAGCCAAGTGAGGACCTTAACCAAGTTTGCACAATGCATGCTAATTGTTGTATTGGTCTTGAAAACAAAGTGAACGATCTTAGAGCTTTGCTTCAAGTTTGGAAAAAATACATGTCATCGCCTCATAATGCTACTGGCACATCACAAGCTGTGTGGACCGTCCCACAAAATTGCAGCACTTCCTTCCAACGCCGTTGGAAGCATAGTAACCAGACTAGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

369

Amino Acids

42.62

Weight (kDa)

8.76

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 123 - 321 4.9e-63 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 750
AccB1I GGYRCC 3 cut(s) 151, 461, 750
AciI CCGC 1 cut(s) 77
AclWI GGATC 4 cut(s) 218, 231, 596, 809
AcsI RAATTY 4 cut(s) 237, 653, 743, 809
AfaI GTAC 2 cut(s) 54, 752
AfiI CCNNNNNNNGG 6 cut(s) 197, 211, 695, 723, 824, 1099
AflIII ACRYGT 2 cut(s) 765, 993
AgsI TTSAA 6 cut(s) 315, 335, 638, 841, 944, 977
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 5 cut(s) 86, 339, 647, 968, 1031
AluI AGCT 5 cut(s) 86, 339, 647, 968, 1031
AlwI GGATC 4 cut(s) 218, 231, 596, 809
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 5 cut(s) 80, 83, 367, 644, 1056
ApoI RAATTY 4 cut(s) 237, 653, 743, 809
Asp718I GGTACC 1 cut(s) 750
AspS9I GGNCC 3 cut(s) 397, 895, 1038
AsuHPI GGTGA 1 cut(s) 674
AvaII GGWCC 3 cut(s) 397, 895, 1038
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 3 cut(s) 151, 461, 750
BbsI GAAGAC 1 cut(s) 531
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 5 cut(s) 92, 95, 354, 656, 1068
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 1059
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciT130I CCWGG 1 cut(s) 129
BciVI GTATCC 4 cut(s) 132, 571, 780, 850
BfaI CTAG 2 cut(s) 383, 1098
BfmI CTRYAG 1 cut(s) 169
BfuI GTATCC 4 cut(s) 132, 571, 780, 850
BglI GCCNNNNNGGC 1 cut(s) 21
BisI GCNGC 6 cut(s) 78, 81, 84, 368, 645, 1057
BlsI GCNGC 6 cut(s) 79, 82, 85, 369, 646, 1058
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 3 cut(s) 397, 895, 1038
BmgT120I GGNCC 3 cut(s) 397, 895, 1038
BmiI GGNNCC 4 cut(s) 153, 225, 463, 752
BmrFI CCNGG 1 cut(s) 129
BmsI GCATC 2 cut(s) 283, 616
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 356, 441
BsaAI YACGTR 1 cut(s) 768
BsaXI ACNNNNNCTCC 4 cut(s) 144, 169, 174, 199
Bsc4I CCNNNNNNNGG 6 cut(s) 197, 211, 695, 723, 824, 1099
Bse118I RCCGGY 2 cut(s) 92, 473
Bse1I ACTGG 3 cut(s) 16, 213, 1021
BseBI CCWGG 1 cut(s) 129
BseLI CCNNNNNNNGG 6 cut(s) 197, 211, 695, 723, 824, 1099
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 3 cut(s) 16, 213, 1021
BseXI GCAGC 5 cut(s) 92, 95, 354, 656, 1068
BshNI GGYRCC 3 cut(s) 151, 461, 750
BsiSI CCGG 2 cut(s) 93, 474
BslFI GGGAC 1 cut(s) 1028
BslI CCNNNNNNNGG 6 cut(s) 197, 211, 695, 723, 824, 1099
BsmFI GGGAC 1 cut(s) 1028
Bsp143I GATC 6 cut(s) 223, 322, 601, 721, 814, 958
BspACI CCGC 1 cut(s) 77
BspCNI CTCAG 1 cut(s) 205
BspLI GGNNCC 4 cut(s) 153, 225, 463, 752
BspMAI CTGCAG 1 cut(s) 173
BspPI GGATC 4 cut(s) 218, 231, 596, 809
BspT107I GGYRCC 3 cut(s) 151, 461, 750
BsrFI RCCGGY 2 cut(s) 92, 473
BsrI ACTGG 3 cut(s) 16, 213, 1021
BssAI RCCGGY 2 cut(s) 92, 473
BssMI GATC 6 cut(s) 223, 322, 601, 721, 814, 958
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 3 cut(s) 11, 271, 1042
BstBAI YACGTR 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 253, 921
BstDEI CTNAG 2 cut(s) 192, 962
BstKTI GATC 6 cut(s) 226, 325, 604, 724, 817, 961
BstMBI GATC 6 cut(s) 223, 322, 601, 721, 814, 958
BstMWI GCNNNNNNNGC 4 cut(s) 21, 83, 89, 373
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 3 cut(s) 255, 923, 997
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 1 cut(s) 169
BstV1I GCAGC 5 cut(s) 92, 95, 354, 656, 1068
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 2 cut(s) 223, 601
BstYI RGATCY 2 cut(s) 223, 601
BsuI GTATCC 4 cut(s) 132, 571, 780, 850
BtgZI GCGATG 1 cut(s) 984
Cac8I GCNNGC 2 cut(s) 253, 921
CaiI CAGNNNCTG 1 cut(s) 86
Cfr10I RCCGGY 2 cut(s) 92, 473
Cfr13I GGNCC 3 cut(s) 397, 895, 1038
CseI GACGC 1 cut(s) 70
Csp6I GTAC 2 cut(s) 53, 751
CspCI CAANNNNNGTGG 4 cut(s) 497, 532, 1016, 1051
CviAII CATG 6 cut(s) 252, 256, 297, 658, 920, 994
CviQI GTAC 2 cut(s) 53, 751
DdeI CTNAG 2 cut(s) 192, 962
DpnI GATC 6 cut(s) 225, 324, 603, 723, 816, 960
DpnII GATC 6 cut(s) 223, 322, 601, 721, 814, 958
DraI TTTAAA 1 cut(s) 679
Eco47I GGWCC 3 cut(s) 397, 895, 1038
EcoO109I RGGNCCY 1 cut(s) 895
EcoRII CCWGG 1 cut(s) 127
EcoT22I ATGCAT 4 cut(s) 253, 257, 298, 921
FaeI CATG 6 cut(s) 255, 259, 300, 661, 923, 997
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 1 cut(s) 1028
FatI CATG 6 cut(s) 251, 255, 296, 657, 919, 993
FauNDI CATATG 1 cut(s) 409
Fnu4HI GCNGC 6 cut(s) 78, 81, 84, 368, 645, 1057
Fsp4HI GCNGC 6 cut(s) 78, 81, 84, 368, 645, 1057
FspBI CTAG 2 cut(s) 383, 1098
GluI GCNGC 6 cut(s) 78, 81, 84, 368, 645, 1057
GsuI CTGGAG 1 cut(s) 230
HapII CCGG 2 cut(s) 93, 474
HgaI GACGC 1 cut(s) 70
Hin1II CATG 6 cut(s) 255, 259, 300, 661, 923, 997
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 3 cut(s) 665, 849, 876
HpaII CCGG 2 cut(s) 93, 474
HphI GGTGA 1 cut(s) 674
Hpy166II GTNNAC 3 cut(s) 397, 955, 1038
Hpy188I TCNGA 3 cut(s) 243, 350, 726
Hpy188III TCNNGA 5 cut(s) 332, 548, 599, 623, 941
Hpy8I GTNNAC 3 cut(s) 397, 955, 1038
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 1075
HpyCH4III ACNGT 3 cut(s) 11, 271, 1042
HpyCH4IV ACGT 2 cut(s) 284, 767
HpyCH4V TGCA 9 cut(s) 171, 251, 255, 296, 629, 644, 912, 919, 1056
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 83, 89, 373
HpyF3I CTNAG 2 cut(s) 192, 962
HpySE526I ACGT 2 cut(s) 284, 767
Hsp92II CATG 6 cut(s) 255, 259, 300, 661, 923, 997
KpnI GGTACC 1 cut(s) 754
Kzo9I GATC 6 cut(s) 223, 322, 601, 721, 814, 958
LmnI GCTCC 1 cut(s) 691
Lsp1109I GCAGC 5 cut(s) 92, 95, 354, 656, 1068
LweI GCATC 2 cut(s) 283, 616
MaeI CTAG 2 cut(s) 383, 1098
MaeII ACGT 2 cut(s) 284, 767
MaeIII GTNAC 3 cut(s) 353, 440, 1088
MalI GATC 6 cut(s) 225, 324, 603, 723, 816, 960
MboI GATC 6 cut(s) 223, 322, 601, 721, 814, 958
MboII GAAGA 4 cut(s) 52, 271, 531, 534
MfeI CAATTG 1 cut(s) 691
MflI RGATCY 2 cut(s) 223, 601
MmeI TCCRAC 2 cut(s) 1058, 1093
Mph1103I ATGCAT 4 cut(s) 253, 257, 298, 921
MseI TTAA 6 cut(s) 287, 342, 678, 798, 807, 900
MslI CAYNNNNRTG 1 cut(s) 392
MspA1I CMGCKG 2 cut(s) 86, 647
MspI CCGG 2 cut(s) 93, 474
MspR9I CCNGG 1 cut(s) 129
MunI CAATTG 1 cut(s) 691
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 21, 83, 89, 373
NdeI CATATG 1 cut(s) 409
NdeII GATC 6 cut(s) 223, 322, 601, 721, 814, 958
NlaIII CATG 6 cut(s) 255, 259, 300, 661, 923, 997
NlaIV GGNNCC 4 cut(s) 153, 225, 463, 752
NsiI ATGCAT 4 cut(s) 253, 257, 298, 921
NspI RCATGY 3 cut(s) 255, 923, 997
PaeI GCATGC 2 cut(s) 255, 923
PciI ACATGT 1 cut(s) 993
PfeI GAWTC 3 cut(s) 665, 849, 876
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 6 cut(s) 79, 82, 85, 369, 646, 1058
Ppu21I YACGTR 1 cut(s) 768
PpuMI RGGWCCY 1 cut(s) 895
PscI ACATGT 1 cut(s) 993
PsiI TTATAA 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 895
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 4 cut(s) 153, 225, 463, 752
PspPI GGNCC 3 cut(s) 397, 895, 1038
PspPPI RGGWCCY 1 cut(s) 895
PstI CTGCAG 1 cut(s) 173
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 2 cut(s) 223, 601
PvuII CAGCTG 2 cut(s) 86, 647
RsaI GTAC 2 cut(s) 54, 752
RsaNI GTAC 2 cut(s) 53, 751
RseI CAYNNNNRTG 1 cut(s) 392
SaqAI TTAA 6 cut(s) 287, 342, 678, 798, 807, 900
SatI GCNGC 6 cut(s) 78, 81, 84, 368, 645, 1057
Sau3AI GATC 6 cut(s) 223, 322, 601, 721, 814, 958
Sau96I GGNCC 3 cut(s) 397, 895, 1038
ScrFI CCNGG 1 cut(s) 129
SfaNI GCATC 2 cut(s) 283, 616
SfcI CTRYAG 1 cut(s) 169
SinI GGWCC 3 cut(s) 397, 895, 1038
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 2 cut(s) 371, 456
SmoI CTYRAG 2 cut(s) 371, 456
SphI GCATGC 2 cut(s) 255, 923
SsiI CCGC 1 cut(s) 77
SspI AATATT 1 cut(s) 318
SspMI CTAG 2 cut(s) 383, 1098
StyD4I CCNGG 1 cut(s) 127
TaaI ACNGT 3 cut(s) 11, 271, 1042
TaiI ACGT 2 cut(s) 287, 770
TaqI TCGA 2 cut(s) 549, 598
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 3 cut(s) 665, 849, 876
Tru1I TTAA 6 cut(s) 287, 342, 678, 798, 807, 900
Tru9I TTAA 6 cut(s) 287, 342, 678, 798, 807, 900
TseI GCWGC 5 cut(s) 80, 83, 367, 644, 1056
TspDTI ATGAA 4 cut(s) 272, 646, 798, 811
TspGWI ACGGA 2 cut(s) 70, 590
VpaK11BI GGWCC 3 cut(s) 397, 895, 1038
XapI RAATTY 4 cut(s) 237, 653, 743, 809
XceI RCATGY 3 cut(s) 255, 923, 997
XspI CTAG 2 cut(s) 383, 1098
Zsp2I ATGCAT 4 cut(s) 253, 257, 298, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.