MD14G1244200.v1.1

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006034
Physical Location & Seq
Forward (+)
32249478 .. 32251447
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1244200.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGAGAAACTCGGCGATTCTTGATATTACTGGGAAGAGGATTAATGTGCGGACGACGCTGGTGTTTATGGCGGCAGCAGCCGTCGCCGGTTGCTTTATTGTCTACAATTTCGTGTACCCTACTGGATTTCGTTATGATGGCTTGGCACCATACCTCCCCTCCTGCAAAACCACAACCCCTCCACTTAAGCAGGGCAACCAGCTGGAGGATATAGATCCATTAGATAAAGTTCTGAAAAATGCATGCATGATGAACAAAACGGTTATAATAACTACGTTAAATGATGCATGGGCAGAACCCAATTCAATATTTGATCTCTTTCTTGAGAGCTTTAACATCGGAAGTAACACGAAATGGCTGCTCAAGCATTTACTAGTCATCTGCGTGGACCAAACAGCATATGCTCGTTGCTTAGCATCACACCCTCATTGTTACTTACTTTACACTCCAGGTGCCAACTTTACCGGTGAGGTATTTTATCTGACTCCCGATTACTTACAGATGATGTGGAGAAGAACCCAGATTTTGTCTTCCATTCTCGACAAAGGCTACAACTTTGTCTTTACGGATACCGATATAATGTGGCTTCGAGATCCATTCCCACACTTCTATCCAGATGCAGATTTTCAAATTGCAACAGATAAATTTCTAGGAGATTCTTATAGTTTTAAAAATCCTCCCAATTGTGGATTTGTCAATGTAAAATCCAATGATCGGACTATTGGGTTTTACAAATTTTGGTACCTCTCCAAAAACACATATCCAAAGACGAATGAGCAAGATGTTCTTAATAGGATAAAATTTGATCCTTTCATTGCGAAGATTGGATTGAAAGTGAGATTCTTGGATACAAAATACTTTAGTGGATTCTGTGACCCAAGTAAGGATGTTAACCAAGTTTGCACAATGCATGCTAATTGTTGTATTGGTCTTGAAAACAAAGTCAACGACCTTAAAGCTTTGCTGCAAGTTTGGAAAAAATACATGTCATCGCCTCGTAACGCCACTGGCACCTCACAAGCTTTATGGACCGTCCCACAAAAATATTGCAGCACTTCCTTCGAACGCCGTTGGAAGCATAATAACCGGAGAAGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

367

Amino Acids

42.22

Weight (kDa)

9.0

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 120 - 318 7e-65 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 266
Acc65I GGTACC 1 cut(s) 741
AccB1I GGYRCC 4 cut(s) 145, 452, 741, 1010
AccI GTMKAC 1 cut(s) 102
AciI CCGC 2 cut(s) 49, 71
AclWI GGATC 3 cut(s) 209, 587, 800
AcsI RAATTY 3 cut(s) 644, 734, 800
AfaI GTAC 2 cut(s) 116, 743
AfiI CCNNNNNNNGG 5 cut(s) 205, 686, 714, 883, 1093
AflII CTTAAG 1 cut(s) 185
AflIII ACRYGT 1 cut(s) 984
AgeI ACCGGT 1 cut(s) 464
AgsI TTSAA 4 cut(s) 306, 629, 832, 935
AhlI ACTAGT 1 cut(s) 373
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 4 cut(s) 202, 330, 959, 1022
AluI AGCT 4 cut(s) 202, 330, 959, 1022
AlwI GGATC 3 cut(s) 209, 587, 800
ApeKI GCWGC 5 cut(s) 74, 77, 358, 964, 1050
ApoI RAATTY 3 cut(s) 644, 734, 800
AseI ATTAAT 1 cut(s) 42
AsiGI ACCGGT 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 741
AspS9I GGNCC 2 cut(s) 388, 1029
AsuHPI GGTGA 1 cut(s) 479
AsuII TTCGAA 1 cut(s) 1062
AvaII GGWCC 2 cut(s) 388, 1029
BanI GGYRCC 4 cut(s) 145, 452, 741, 1010
BbsI GAAGAC 1 cut(s) 522
BbvI GCAGC 5 cut(s) 86, 89, 345, 951, 1062
BccI CCATC 1 cut(s) 131
BceAI ACGGC 2 cut(s) 65, 1053
BcgI CGANNNNNNTGC 4 cut(s) 340, 374, 1042, 1076
BciT130I CCWGG 1 cut(s) 450
BciVI GTATCC 2 cut(s) 562, 841
BcuI ACTAGT 1 cut(s) 373
BfaI CTAG 2 cut(s) 374, 650
BfrI CTTAAG 1 cut(s) 185
BfuI GTATCC 2 cut(s) 562, 841
BisI GCNGC 6 cut(s) 72, 75, 78, 359, 965, 1051
BlpI GCTNAGC 1 cut(s) 412
BlsI GCNGC 6 cut(s) 73, 76, 79, 360, 966, 1052
Bme1390I CCNGG 1 cut(s) 450
Bme18I GGWCC 2 cut(s) 388, 1029
BmgT120I GGNCC 2 cut(s) 388, 1029
BmiI GGNNCC 4 cut(s) 147, 454, 743, 1012
BmrFI CCNGG 1 cut(s) 450
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 3 cut(s) 274, 425, 607
BmuI ACTGGG 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 522
BpmI CTGGAG 2 cut(s) 224, 432
Bpu1102I GCTNAGC 1 cut(s) 412
Bpu14I TTCGAA 1 cut(s) 1062
BpuEI CTTGAG 2 cut(s) 344, 347
BsaBI GATNNNNATC 1 cut(s) 213
BsaWI WCCGGW 2 cut(s) 464, 1086
BsaXI ACNNNNNCTCC 6 cut(s) 138, 143, 163, 168, 173, 193
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 686, 714, 883, 1093
Bse118I RCCGGY 2 cut(s) 86, 464
Bse1I ACTGG 3 cut(s) 34, 127, 1012
Bse3DI GCAATG 1 cut(s) 813
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 1 cut(s) 450
BseGI GGATG 1 cut(s) 892
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 5 cut(s) 205, 686, 714, 883, 1093
BseMI GCAATG 1 cut(s) 813
BseNI ACTGG 3 cut(s) 34, 127, 1012
BseXI GCAGC 5 cut(s) 86, 89, 345, 951, 1062
BshNI GGYRCC 4 cut(s) 145, 452, 741, 1010
BshTI ACCGGT 1 cut(s) 464
BsiSI CCGG 3 cut(s) 87, 465, 1087
BslFI GGGAC 1 cut(s) 1019
BslI CCNNNNNNNGG 5 cut(s) 205, 686, 714, 883, 1093
BsmFI GGGAC 1 cut(s) 1019
Bsp119I TTCGAA 1 cut(s) 1062
Bsp143I GATC 5 cut(s) 214, 313, 592, 712, 805
Bsp1720I GCTNAGC 1 cut(s) 412
BspACI CCGC 2 cut(s) 49, 71
BspLI GGNNCC 4 cut(s) 147, 454, 743, 1012
BspPI GGATC 3 cut(s) 209, 587, 800
BspT104I TTCGAA 1 cut(s) 1062
BspT107I GGYRCC 4 cut(s) 145, 452, 741, 1010
BspTI CTTAAG 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 813
BsrFI RCCGGY 2 cut(s) 86, 464
BsrI ACTGG 3 cut(s) 34, 127, 1012
BssAI RCCGGY 2 cut(s) 86, 464
BssMI GATC 5 cut(s) 214, 313, 592, 712, 805
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 2 cut(s) 262, 1033
Bst6I CTCTTC 1 cut(s) 29
BstAFI CTTAAG 1 cut(s) 185
BstBI TTCGAA 1 cut(s) 1062
BstC8I GCNNGC 2 cut(s) 244, 912
BstDEI CTNAG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 892
BstKTI GATC 5 cut(s) 217, 316, 595, 715, 808
BstMBI GATC 5 cut(s) 214, 313, 592, 712, 805
BstMWI GCNNNNNNNGC 4 cut(s) 55, 77, 83, 364
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 3 cut(s) 246, 914, 988
BstSCI CCNGG 1 cut(s) 448
BstV1I GCAGC 5 cut(s) 86, 89, 345, 951, 1062
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 2 cut(s) 214, 592
BstYI RGATCY 2 cut(s) 214, 592
BsuI GTATCC 2 cut(s) 562, 841
BtgZI GCGATG 1 cut(s) 975
BtsCI GGATG 1 cut(s) 892
BtsIMutI CAGTG 1 cut(s) 1005
Cac8I GCNNGC 2 cut(s) 244, 912
Cfr10I RCCGGY 2 cut(s) 86, 464
Cfr13I GGNCC 2 cut(s) 388, 1029
CseI GACGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 115, 742
CspAI ACCGGT 1 cut(s) 464
CviAII CATG 5 cut(s) 243, 247, 288, 911, 985
CviJI RGCY 9 cut(s) 80, 141, 202, 330, 358, 549, 586, 959, 1022
CviKI_1 RGCY 9 cut(s) 80, 141, 202, 330, 358, 549, 586, 959, 1022
CviQI GTAC 2 cut(s) 115, 742
DdeI CTNAG 1 cut(s) 412
DpnI GATC 5 cut(s) 216, 315, 594, 714, 807
DpnII GATC 5 cut(s) 214, 313, 592, 712, 805
DraI TTTAAA 1 cut(s) 670
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco47I GGWCC 2 cut(s) 388, 1029
EcoRII CCWGG 1 cut(s) 448
EcoT22I ATGCAT 4 cut(s) 244, 248, 289, 912
FaeI CATG 5 cut(s) 246, 250, 291, 914, 988
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 1 cut(s) 1019
FatI CATG 5 cut(s) 242, 246, 287, 910, 984
FauNDI CATATG 1 cut(s) 400
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 6 cut(s) 72, 75, 78, 359, 965, 1051
FokI GGATG 1 cut(s) 899
Fsp4HI GCNGC 6 cut(s) 72, 75, 78, 359, 965, 1051
FspBI CTAG 2 cut(s) 374, 650
GluI GCNGC 6 cut(s) 72, 75, 78, 359, 965, 1051
GsuI CTGGAG 2 cut(s) 224, 432
HapII CCGG 3 cut(s) 87, 465, 1087
HgaI GACGC 1 cut(s) 64
Hin1II CATG 5 cut(s) 246, 250, 291, 914, 988
HincII GTYRAC 2 cut(s) 892, 946
HindII GTYRAC 2 cut(s) 892, 946
HindIII AAGCTT 2 cut(s) 957, 1020
HinfI GANTC 5 cut(s) 16, 484, 656, 840, 867
HpaI GTTAAC 1 cut(s) 892
HpaII CCGG 3 cut(s) 87, 465, 1087
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 5 cut(s) 103, 115, 388, 892, 946
Hpy188I TCNGA 4 cut(s) 234, 341, 483, 717
Hpy188III TCNNGA 7 cut(s) 20, 323, 488, 539, 590, 614, 932
Hpy8I GTNNAC 5 cut(s) 103, 115, 388, 892, 946
Hpy99I CGWCG 2 cut(s) 58, 86
HpyAV CCTTC 2 cut(s) 1069, 1086
HpyCH4III ACNGT 2 cut(s) 262, 1033
HpyCH4IV ACGT 1 cut(s) 275
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 77, 83, 364
HpyF3I CTNAG 1 cut(s) 412
HpySE526I ACGT 1 cut(s) 275
Hsp92II CATG 5 cut(s) 246, 250, 291, 914, 988
KpnI GGTACC 1 cut(s) 745
KspAI GTTAAC 1 cut(s) 892
Kzo9I GATC 5 cut(s) 214, 313, 592, 712, 805
Lsp1109I GCAGC 5 cut(s) 86, 89, 345, 951, 1062
LweI GCATC 3 cut(s) 274, 425, 607
MaeI CTAG 2 cut(s) 374, 650
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 4 cut(s) 344, 431, 872, 998
MalI GATC 5 cut(s) 216, 315, 594, 714, 807
MboI GATC 5 cut(s) 214, 313, 592, 712, 805
MboII GAAGA 4 cut(s) 46, 522, 525, 832
MfeI CAATTG 1 cut(s) 682
MflI RGATCY 2 cut(s) 214, 592
MluCI AATT 8 cut(s) 106, 301, 630, 644, 682, 734, 800, 916
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 1052
Mph1103I ATGCAT 4 cut(s) 244, 248, 289, 912
MseI TTAA 8 cut(s) 42, 186, 278, 333, 669, 789, 891, 954
MslI CAYNNNNRTG 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 202
MspCI CTTAAG 1 cut(s) 185
MspI CCGG 3 cut(s) 87, 465, 1087
MspR9I CCNGG 1 cut(s) 450
MunI CAATTG 1 cut(s) 682
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 4 cut(s) 55, 77, 83, 364
NdeI CATATG 1 cut(s) 400
NdeII GATC 5 cut(s) 214, 313, 592, 712, 805
NlaIII CATG 5 cut(s) 246, 250, 291, 914, 988
NlaIV GGNNCC 4 cut(s) 147, 454, 743, 1012
NmuCI GTSAC 1 cut(s) 872
NsiI ATGCAT 4 cut(s) 244, 248, 289, 912
NspI RCATGY 3 cut(s) 246, 914, 988
NspV TTCGAA 1 cut(s) 1062
PaeI GCATGC 2 cut(s) 246, 914
PciI ACATGT 1 cut(s) 984
PfeI GAWTC 4 cut(s) 16, 656, 840, 867
PinAI ACCGGT 1 cut(s) 464
PkrI GCNGC 6 cut(s) 73, 76, 79, 360, 966, 1052
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PscI ACATGT 1 cut(s) 984
PshBI ATTAAT 1 cut(s) 42
PsiI TTATAA 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 4 cut(s) 147, 454, 743, 1012
PspPI GGNCC 2 cut(s) 388, 1029
PsuI RGATCY 2 cut(s) 214, 592
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 2 cut(s) 116, 743
RsaNI GTAC 2 cut(s) 115, 742
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 8 cut(s) 42, 186, 278, 333, 669, 789, 891, 954
SatI GCNGC 6 cut(s) 72, 75, 78, 359, 965, 1051
Sau3AI GATC 5 cut(s) 214, 313, 592, 712, 805
Sau96I GGNCC 2 cut(s) 388, 1029
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 450
SfaNI GCATC 3 cut(s) 274, 425, 607
SfuI TTCGAA 1 cut(s) 1062
SinI GGWCC 2 cut(s) 388, 1029
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 3 cut(s) 185, 323, 362
SmoI CTYRAG 3 cut(s) 185, 323, 362
SpeI ACTAGT 1 cut(s) 373
SphI GCATGC 2 cut(s) 246, 914
Sse9I AATT 8 cut(s) 106, 301, 630, 644, 682, 734, 800, 916
SsiI CCGC 2 cut(s) 49, 71
SspI AATATT 2 cut(s) 309, 1046
SspMI CTAG 2 cut(s) 374, 650
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 2 cut(s) 262, 1033
TaiI ACGT 1 cut(s) 278
TaqI TCGA 3 cut(s) 540, 589, 1062
TasI AATT 8 cut(s) 106, 301, 630, 644, 682, 734, 800, 916
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 4 cut(s) 16, 656, 840, 867
Tru1I TTAA 8 cut(s) 42, 186, 278, 333, 669, 789, 891, 954
Tru9I TTAA 8 cut(s) 42, 186, 278, 333, 669, 789, 891, 954
TscAI CASTG 1 cut(s) 1012
TseFI GTSAC 1 cut(s) 872
TseI GCWGC 5 cut(s) 74, 77, 358, 964, 1050
Tsp45I GTSAC 1 cut(s) 872
TspDTI ATGAA 2 cut(s) 266, 802
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 1 cut(s) 1012
Vha464I CTTAAG 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 388, 1029
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 3 cut(s) 644, 734, 800
XceI RCATGY 3 cut(s) 246, 914, 988
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 2 cut(s) 374, 650
Zsp2I ATGCAT 4 cut(s) 244, 248, 289, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.