pycom14g20530

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006034
Physical Location & Seq
Forward (+)
21566409 .. 21568917
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g20530.1

Sequence Viewer

Length: 1194 bp
ATGAGCAAATTATCGGTAGATGAAGCAGCTGGAAATTATGTTAGCGGGAATATGATGAAATTACTATCATCATCATCATCATCATCATCATCGTCATCACCATCATCCAACGGTGGAAACGGATCATCAGGTAGCAGTAATCGAGCGTTGGTGGCGAAGAAGTTTCTAAAGGTGACGTTGCTGTTTGCGGCAGCCACTGTGTGTTGCTTTGGCTTCTACAATTCTGTCTACACTTCCGGATTTCTGCTCAGATCCTCTTATGACTACGCTTTGTCTGCCACCAGAACCGTAACTTCTCATCAGACCAAAGTAGAAGATCCATTGGACACAGTTCTCAGAAATGCATCCATGAAGAACAAAACCGTTATCGTAACTACTCTAAATGATGCATGGGCAGAACCCAATTCAATATTCGATTCCTTTTTAGAGAGTTTCGAGATCGGAAGTAACACAAAATGGCTGCTCAAGCATTTGGTGGTGATATGCTTGGACAAAAAAGCATATGCGCGTTGCTTGGCATCACACCCTCATTGCTACCAACTCTACACTCAAGGTGCCAATTTCACCAGCGAAGCTGCTTTCATGTCTTTAAAGTACCTGGACATGATGTGGAGACGAATCCAATTTTTGTCTTCTATTCTCGACAGAGGCTACAATTTTGTCTTTACAGACGCTGATATAATGTGGCTACGGAATCCATTCCCACGATTGTATCCAGATGCAGATTTTCAAATTGCATGCGATTACTTCCTCGGTGATTCTTATAGCATGAAGAACTTTCCCAATGGAGGGTTTACTTATGTAAAATCAAACAATCGCACGATTCAATTTTATAAGTTTTGGTACTTCTCAAGAAAGGCGTATCCAAAGATGCATGACCAAGACGTTCTCAATGAGATCAAATTTGATCCCTTCCTCACCCAAATTGGATTGAAAATGAGGTTCTTGGATACACTATACTTTGGTGGCTTCTGCCAGGCGAGTAAGGACTTTAACCAAGTTTGCACAATGCATGCCAATTGTTGTGTTGGTCTCGAAAACTTGCAGCAAAAGTCAATCGATCTTAAAATTTTGCTTCAAGTTTGGAGAAAGTACATGTCCTTACCTCCTAATGCAACTGCCACACCACAGCTTTCATGGACTGTTCCACAAAATTGCAGCACTTCCTTCCAACGCCTTGAAAAGCAATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

398

Amino Acids

44.98

Weight (kDa)

8.97

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 154 - 347 2.9e-69 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AccB1I GGYRCC 1 cut(s) 554
AccI GTMKAC 1 cut(s) 228
AccII CGCG 1 cut(s) 508
AccIII TCCGGA 1 cut(s) 236
AciI CCGC 2 cut(s) 45, 188
AclWI GGATC 4 cut(s) 130, 246, 311, 902
AcsI RAATTY 2 cut(s) 902, 1068
AdeI CACNNNGTG 1 cut(s) 201
AfaI GTAC 3 cut(s) 596, 845, 1094
AfiI CCNNNNNNNGG 2 cut(s) 788, 789
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 6 cut(s) 408, 731, 827, 934, 1079, 1181
AjnI CCWGG 2 cut(s) 597, 975
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AluBI AGCT 3 cut(s) 29, 575, 1132
AluI AGCT 3 cut(s) 29, 575, 1132
Alw26I GTCTC 2 cut(s) 607, 1037
AlwI GGATC 4 cut(s) 130, 246, 311, 902
AlwNI CAGNNNCTG 2 cut(s) 197, 674
Aor13HI TCCGGA 1 cut(s) 236
ApeKI GCWGC 6 cut(s) 26, 191, 460, 575, 1045, 1158
ApoI RAATTY 2 cut(s) 902, 1068
Asp700I GAANNNNTTC 1 cut(s) 698
AspLEI GCGC 1 cut(s) 508
AsuHPI GGTGA 6 cut(s) 90, 184, 490, 556, 767, 910
BaeI ACNNNNGTAYC 2 cut(s) 695, 728
BanI GGYRCC 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 624
BbvI GCAGC 6 cut(s) 38, 203, 447, 562, 1057, 1170
BccI CCATC 1 cut(s) 109
BciT130I CCWGG 2 cut(s) 599, 977
BciVI GTATCC 3 cut(s) 723, 873, 943
BcoDI GTCTC 2 cut(s) 607, 1037
BfuI GTATCC 3 cut(s) 723, 873, 943
BisI GCNGC 7 cut(s) 27, 189, 192, 461, 576, 1046, 1159
BlsI GCNGC 7 cut(s) 28, 190, 193, 462, 577, 1047, 1160
Bme1390I CCNGG 2 cut(s) 599, 977
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 2 cut(s) 599, 977
BmsI GCATC 5 cut(s) 353, 376, 527, 709, 861
BpiI GAAGAC 1 cut(s) 624
BpuEI CTTGAG 3 cut(s) 449, 534, 835
Bsa29I ATCGAT 1 cut(s) 1059
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 1 cut(s) 751
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 2 cut(s) 788, 789
Bse3DI GCAATG 1 cut(s) 529
BseAI TCCGGA 1 cut(s) 236
BseBI CCWGG 2 cut(s) 599, 977
BseCI ATCGAT 1 cut(s) 1059
BseDI CCNNGG 1 cut(s) 751
BseGI GGATG 2 cut(s) 104, 344
BseLI CCNNNNNNNGG 2 cut(s) 788, 789
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 2 cut(s) 262, 349
BseXI GCAGC 6 cut(s) 38, 203, 447, 562, 1057, 1170
Bsh1236I CGCG 1 cut(s) 508
BshNI GGYRCC 1 cut(s) 554
BshVI ATCGAT 1 cut(s) 1059
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 788, 789
BsmAI GTCTC 2 cut(s) 607, 1037
BsmBI CGTCTC 1 cut(s) 607
Bso31I GGTCTC 1 cut(s) 1037
Bsp13I TCCGGA 1 cut(s) 236
Bsp143I GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
BspACI CCGC 2 cut(s) 45, 188
BspCNI CTCAG 2 cut(s) 261, 348
BspDI ATCGAT 1 cut(s) 1059
BspEI TCCGGA 1 cut(s) 236
BspFNI CGCG 1 cut(s) 508
BspLI GGNNCC 1 cut(s) 556
BspPI GGATC 4 cut(s) 130, 246, 311, 902
BspT107I GGYRCC 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 1037
BsrDI GCAATG 1 cut(s) 529
BssECI CCNNGG 1 cut(s) 751
BssMI GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
Bst2UI CCWGG 2 cut(s) 599, 977
Bst4CI ACNGT 6 cut(s) 113, 199, 289, 331, 364, 1144
BstC8I GCNNGC 2 cut(s) 739, 1014
BstDEI CTNAG 2 cut(s) 248, 335
BstF5I GGATG 2 cut(s) 104, 344
BstFNI CGCG 1 cut(s) 508
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 7 cut(s) 125, 254, 319, 441, 900, 910, 1063
BstMAI GTCTC 2 cut(s) 607, 1037
BstMBI GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
BstMWI GCNNNNNNNGC 3 cut(s) 152, 275, 466
BstNI CCWGG 2 cut(s) 599, 977
BstNSI RCATGY 3 cut(s) 741, 1016, 1099
BstSCI CCNGG 2 cut(s) 597, 975
BstUI CGCG 1 cut(s) 508
BstV1I GCAGC 6 cut(s) 38, 203, 447, 562, 1057, 1170
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 2 cut(s) 251, 316
BstYI RGATCY 2 cut(s) 251, 316
Bsu15I ATCGAT 1 cut(s) 1059
BsuI GTATCC 3 cut(s) 723, 873, 943
BsuTUI ATCGAT 1 cut(s) 1059
BtsCI GGATG 2 cut(s) 104, 344
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 739, 1014
CaiI CAGNNNCTG 2 cut(s) 197, 674
CfoI GCGC 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 1059
CseI GACGC 1 cut(s) 680
Csp6I GTAC 3 cut(s) 595, 844, 1093
CviJI RGCY 9 cut(s) 29, 194, 213, 460, 575, 651, 688, 969, 1132
CviKI_1 RGCY 9 cut(s) 29, 194, 213, 460, 575, 651, 688, 969, 1132
CviQI GTAC 3 cut(s) 595, 844, 1093
DdeI CTNAG 2 cut(s) 248, 335
DpnI GATC 7 cut(s) 124, 253, 318, 440, 899, 909, 1062
DpnII GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
DraI TTTAAA 1 cut(s) 591
DraIII CACNNNGTG 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 1037
EcoRII CCWGG 2 cut(s) 597, 975
EcoT22I ATGCAT 4 cut(s) 346, 391, 876, 1014
Esp3I CGTCTC 1 cut(s) 607
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 502
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 7 cut(s) 27, 189, 192, 461, 576, 1046, 1159
FokI GGATG 2 cut(s) 91, 331
Fsp4HI GCNGC 7 cut(s) 27, 189, 192, 461, 576, 1046, 1159
GlaI GCGC 1 cut(s) 507
GluI GCNGC 7 cut(s) 27, 189, 192, 461, 576, 1046, 1159
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 680
HhaI GCGC 1 cut(s) 508
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HinfI GANTC 5 cut(s) 416, 618, 694, 758, 823
HpaII CCGG 1 cut(s) 237
HphI GGTGA 6 cut(s) 90, 184, 490, 556, 767, 910
Hpy166II GTNNAC 2 cut(s) 229, 795
Hpy188I TCNGA 4 cut(s) 251, 303, 338, 443
Hpy188III TCNNGA 7 cut(s) 237, 436, 641, 716, 852, 1034, 1191
Hpy8I GTNNAC 2 cut(s) 229, 795
HpyAV CCTTC 2 cut(s) 922, 1177
HpyCH4III ACNGT 6 cut(s) 113, 199, 289, 331, 364, 1144
HpyCH4IV ACGT 2 cut(s) 176, 885
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 275, 466
HpyF3I CTNAG 2 cut(s) 248, 335
HpySE526I ACGT 2 cut(s) 176, 885
HspAI GCGC 1 cut(s) 506
Kpn2I TCCGGA 1 cut(s) 236
Kzo9I GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
Lsp1109I GCAGC 6 cut(s) 38, 203, 447, 562, 1057, 1170
LweI GCATC 5 cut(s) 353, 376, 527, 709, 861
MaeII ACGT 2 cut(s) 176, 885
MaeIII GTNAC 4 cut(s) 172, 289, 370, 446
MalI GATC 7 cut(s) 124, 253, 318, 440, 899, 909, 1062
MboI GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
MboII GAAGA 5 cut(s) 169, 326, 364, 624, 784
MfeI CAATTG 1 cut(s) 1018
MflI RGATCY 2 cut(s) 251, 316
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 8 cut(s) 265, 537, 641, 761, 782, 926, 933, 1116
Mph1103I ATGCAT 4 cut(s) 346, 391, 876, 1014
MroI TCCGGA 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 698
MseI TTAA 3 cut(s) 590, 993, 1065
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 2 cut(s) 599, 977
MunI CAATTG 1 cut(s) 1018
MvaI CCWGG 2 cut(s) 599, 977
MvnI CGCG 1 cut(s) 508
MwoI GCNNNNNNNGC 3 cut(s) 152, 275, 466
NdeI CATATG 1 cut(s) 502
NdeII GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
NlaIV GGNNCC 1 cut(s) 556
NmuCI GTSAC 1 cut(s) 172
NsiI ATGCAT 4 cut(s) 346, 391, 876, 1014
NspI RCATGY 3 cut(s) 741, 1016, 1099
PaeI GCATGC 2 cut(s) 741, 1016
PciI ACATGT 1 cut(s) 1095
PdmI GAANNNNTTC 1 cut(s) 698
PfeI GAWTC 5 cut(s) 416, 618, 694, 758, 823
PkrI GCNGC 7 cut(s) 28, 190, 193, 462, 577, 1047, 1160
PscI ACATGT 1 cut(s) 1095
PsiI TTATAA 1 cut(s) 834
Psp6I CCWGG 2 cut(s) 597, 975
PspGI CCWGG 2 cut(s) 597, 975
PspN4I GGNNCC 1 cut(s) 556
PstNI CAGNNNCTG 2 cut(s) 197, 674
PsuI RGATCY 2 cut(s) 251, 316
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 3 cut(s) 596, 845, 1094
RsaNI GTAC 3 cut(s) 595, 844, 1093
SaqAI TTAA 3 cut(s) 590, 993, 1065
SatI GCNGC 7 cut(s) 27, 189, 192, 461, 576, 1046, 1159
Sau3AI GATC 7 cut(s) 122, 251, 316, 438, 897, 907, 1060
ScrFI CCNGG 2 cut(s) 599, 977
SfaNI GCATC 5 cut(s) 353, 376, 527, 709, 861
SmlI CTYRAG 3 cut(s) 464, 549, 850
SmoI CTYRAG 3 cut(s) 464, 549, 850
SphI GCATGC 2 cut(s) 741, 1016
SsiI CCGC 2 cut(s) 45, 188
SspI AATATT 1 cut(s) 411
StyD4I CCNGG 2 cut(s) 597, 975
TaaI ACNGT 6 cut(s) 113, 199, 289, 331, 364, 1144
TaiI ACGT 2 cut(s) 179, 888
TaqI TCGA 6 cut(s) 142, 414, 435, 642, 1035, 1059
TatI WGTACW 1 cut(s) 1092
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 5 cut(s) 416, 618, 694, 758, 823
Tru1I TTAA 3 cut(s) 590, 993, 1065
Tru9I TTAA 3 cut(s) 590, 993, 1065
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 6 cut(s) 26, 191, 460, 575, 1045, 1158
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 6 cut(s) 36, 71, 365, 571, 785, 1125
TspGWI ACGGA 2 cut(s) 135, 706
TspRI CASTG 1 cut(s) 202
XapI RAATTY 2 cut(s) 902, 1068
XceI RCATGY 3 cut(s) 741, 1016, 1099
XcmI CCANNNNNNNNNTGG 1 cut(s) 1134
XmiI GTMKAC 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 698
Zsp2I ATGCAT 4 cut(s) 346, 391, 876, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.