Rroxscaffold_3G00275890

At4g15970-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006034
Physical Location & Seq
Forward (+)
67197420 .. 67200291
2872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275890.1

Sequence Viewer

Length: 1110 bp
ATGAGCAGTAAGTCATTATCACCAGTAGCAGCAGTATTATCTGTAACAGTTATTGGGAATAACAGTACTGGTGTTGTGGAGAATAACACAAAAGCTGCAACACCAAAGGCTGGTGGTGTTCATCACCATTATCATCATCATCATCATGGATTGGTCAAGAGGATTCTAAATATTGCTCTGGATCCCTTGGATGCAGTTCTGAGAAATGCATCCATGAAGGACAAGACCATAATCGTCACAACACTGAATGATGCGTGGGCAGAACCCAACTCAATATTCGATCTGTTTCTCGAGAGTTTTCGGATCGGAAACAACACGAATCGGCTGCTGAAGCATCTGGTGGTGATCTGCTTGGACCAAAAGGCATACGCTCGTTGCTTGGCTCTTCACCCTCATTGCTACCAACTCTACACTCAAGGTGCCAACTTTACCAGCGAGGCAGCTTTCATGTCCTCCGATTACTTGCACATGATGTGGAGAAGAATCCAGTTTTTGACTTCTATTCTCGAAAAAGGATACAGCTTTGTCTTTACGGACACTGACATTATGTGGCTTAGGGACCCATTCCCACAATTCTTTCCGGATGCAGACTTCCAAATTGCATGCGACTATTTCCGAGGCGATTCTTACGATGTGAACAACCTACCTAATGGAGGATTTACCTATGTAGTCTCTAATAAACGGACCACCCAATTCTACAACTTTTGGTACTTCTCAAGGCAGGCGTATCCGAATAATCACGACCAGGACGTGCTCAACAAGATCAAATCTGATCCGTTCATCACCAGCATCGGATTGAAAATGAGATTCTTGGACACCAAATACTTTGGCGGCTTCTGTCAGCCCAGTAAAGATTTAAACCTGGTTTGCACAATGCACGCTAATTGCTGTGTTGGTCTCGATAACAAAGTCAATGACCTCAAAATTCTGCTTCAAGATTGGAGAAATTACGTGGCATTGCCTCCTTCTCCTTCTTCATCTTCTTCCGGTCCTAATTCTAATACCACCACGCCTCCAACAACTAGGCCCTCATGGACCGTTCCACAGAACTGCAGGTTAAAAGACAAAACGATTGTGAAGTCCAGCAATGAAATTCATACCATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleotid_trans

Protein Analysis

369

Amino Acids

41.85

Weight (kDa)

8.62

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 109 - 307 7.2e-69 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1044
AccB1I GGYRCC 1 cut(s) 419
AccB7I CCANNNNNTGG 1 cut(s) 110
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 1 cut(s) 831
AclWI GGATC 4 cut(s) 176, 189, 311, 767
AcsI RAATTY 2 cut(s) 924, 1092
AcuI CTGAAG 1 cut(s) 350
AfaI GTAC 2 cut(s) 67, 710
AfiI CCNNNNNNNGG 2 cut(s) 110, 653
AgsI TTSAA 2 cut(s) 799, 935
AjiI CACGTC 1 cut(s) 751
AjnI CCWGG 2 cut(s) 744, 861
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AluBI AGCT 3 cut(s) 95, 443, 522
AluI AGCT 3 cut(s) 95, 443, 522
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 2 cut(s) 676, 902
AlwI GGATC 4 cut(s) 176, 189, 311, 767
Ama87I CYCGRG 1 cut(s) 290
Aor13HI TCCGGA 1 cut(s) 580
AoxI GGCC 1 cut(s) 1025
ApeKI GCWGC 4 cut(s) 29, 95, 325, 440
ApoI RAATTY 2 cut(s) 924, 1092
AspS9I GGNCC 6 cut(s) 355, 559, 684, 989, 1026, 1035
AsuHPI GGTGA 5 cut(s) 12, 116, 355, 380, 775
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 5 cut(s) 355, 559, 684, 989, 1035
BamHI GGATCC 1 cut(s) 181
BanI GGYRCC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 756
BbvI GCAGC 4 cut(s) 41, 82, 312, 452
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 2 cut(s) 746, 863
BciVI GTATCC 2 cut(s) 509, 738
BcoDI GTCTC 2 cut(s) 676, 902
BfaI CTAG 1 cut(s) 1023
BfmI CTRYAG 1 cut(s) 1051
BfuAI ACCTGC 1 cut(s) 1044
BfuI GTATCC 2 cut(s) 509, 738
BisI GCNGC 5 cut(s) 30, 96, 326, 441, 832
BlsI GCNGC 5 cut(s) 31, 97, 327, 442, 833
BmcAI AGTACT 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 746, 863
Bme18I GGWCC 5 cut(s) 355, 559, 684, 989, 1035
BmeT110I CYCGRG 1 cut(s) 290
BmgBI CACGTC 1 cut(s) 751
BmgT120I GGNCC 6 cut(s) 355, 559, 684, 989, 1026, 1035
BmiI GGNNCC 4 cut(s) 183, 421, 560, 561
BmrFI CCNGG 2 cut(s) 746, 863
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 6 cut(s) 181, 218, 241, 343, 574, 798
BmuI ACTGGG 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 554
BpuEI CTTGAG 2 cut(s) 399, 700
BsaAI YACGTR 1 cut(s) 952
BsaI GGTCTC 1 cut(s) 902
BsaJI CCNNGG 2 cut(s) 186, 616
BsaWI WCCGGW 2 cut(s) 580, 986
BsaXI ACNNNNNCTCC 2 cut(s) 997, 1027
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 653
Bse1I ACTGG 4 cut(s) 23, 73, 487, 846
Bse3DI GCAATG 3 cut(s) 394, 956, 1093
BseAI TCCGGA 1 cut(s) 580
BseBI CCWGG 2 cut(s) 746, 863
BseDI CCNNGG 2 cut(s) 186, 616
BseGI GGATG 3 cut(s) 196, 209, 589
BseLI CCNNNNNNNGG 2 cut(s) 110, 653
BseMI GCAATG 3 cut(s) 394, 956, 1093
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 4 cut(s) 23, 73, 487, 846
BseXI GCAGC 4 cut(s) 41, 82, 312, 452
BshFI GGCC 1 cut(s) 1027
BshNI GGYRCC 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 2 cut(s) 581, 987
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 2 cut(s) 110, 653
BsmAI GTCTC 2 cut(s) 676, 902
BsmFI GGGAC 1 cut(s) 572
BsnI GGCC 1 cut(s) 1027
Bso31I GGTCTC 1 cut(s) 902
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 756
Bsp13I TCCGGA 1 cut(s) 580
Bsp143I GATC 6 cut(s) 181, 280, 303, 345, 762, 772
BspACI CCGC 1 cut(s) 831
BspANI GGCC 1 cut(s) 1027
BspCNI CTCAG 1 cut(s) 192
BspEI TCCGGA 1 cut(s) 580
BspLI GGNNCC 4 cut(s) 183, 421, 560, 561
BspMAI CTGCAG 1 cut(s) 1055
BspMI ACCTGC 1 cut(s) 1044
BspPI GGATC 4 cut(s) 176, 189, 311, 767
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 902
BsrDI GCAATG 3 cut(s) 394, 956, 1093
BsrI ACTGG 4 cut(s) 23, 73, 487, 846
BssECI CCNNGG 2 cut(s) 186, 616
BssMI GATC 6 cut(s) 181, 280, 303, 345, 762, 772
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 746, 863
Bst4CI ACNGT 3 cut(s) 49, 65, 1039
Bst6I CTCTTC 1 cut(s) 390
BstBAI YACGTR 1 cut(s) 952
BstC8I GCNNGC 3 cut(s) 604, 723, 879
BstDEI CTNAG 2 cut(s) 200, 554
BstENI CCTNNNNNAGG 1 cut(s) 651
BstF5I GGATG 3 cut(s) 196, 209, 589
BstKTI GATC 6 cut(s) 184, 283, 306, 348, 765, 775
BstMAI GTCTC 2 cut(s) 676, 902
BstMBI GATC 6 cut(s) 181, 280, 303, 345, 762, 772
BstMWI GCNNNNNNNGC 1 cut(s) 331
BstNI CCWGG 2 cut(s) 746, 863
BstNSI RCATGY 1 cut(s) 606
BstSCI CCNGG 2 cut(s) 744, 861
BstSFI CTRYAG 1 cut(s) 1051
BstV1I GCAGC 4 cut(s) 41, 82, 312, 452
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuI GTATCC 2 cut(s) 509, 738
BsuRI GGCC 1 cut(s) 1027
BtrI CACGTC 1 cut(s) 751
BtsCI GGATG 3 cut(s) 196, 209, 589
BtsIMutI CAGTG 2 cut(s) 242, 537
BveI ACCTGC 1 cut(s) 1044
Cac8I GCNNGC 3 cut(s) 604, 723, 879
Cfr13I GGNCC 6 cut(s) 355, 559, 684, 989, 1026, 1035
CsiI ACCWGGT 1 cut(s) 861
Csp6I GTAC 2 cut(s) 66, 709
CviAII CATG 6 cut(s) 146, 214, 448, 469, 603, 1032
CviQI GTAC 2 cut(s) 66, 709
DdeI CTNAG 2 cut(s) 200, 554
DpnI GATC 6 cut(s) 183, 282, 305, 347, 764, 774
DpnII GATC 6 cut(s) 181, 280, 303, 345, 762, 772
DraI TTTAAA 1 cut(s) 858
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 5 cut(s) 355, 559, 684, 989, 1035
Eco57I CTGAAG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 651
EcoO109I RGGNCCY 2 cut(s) 559, 1026
EcoRII CCWGG 2 cut(s) 744, 861
EcoT14I CCWWGG 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 6 cut(s) 149, 217, 451, 472, 606, 1035
FaqI GGGAC 1 cut(s) 572
FatI CATG 6 cut(s) 145, 213, 447, 468, 602, 1031
Fnu4HI GCNGC 5 cut(s) 30, 96, 326, 441, 832
FokI GGATG 3 cut(s) 196, 203, 596
Fsp4HI GCNGC 5 cut(s) 30, 96, 326, 441, 832
FspBI CTAG 1 cut(s) 1023
GluI GCNGC 5 cut(s) 30, 96, 326, 441, 832
HaeIII GGCC 1 cut(s) 1027
HapII CCGG 2 cut(s) 581, 987
Hin1II CATG 6 cut(s) 149, 217, 451, 472, 606, 1035
HinfI GANTC 5 cut(s) 163, 319, 483, 623, 807
HpaII CCGG 2 cut(s) 581, 987
HphI GGTGA 5 cut(s) 12, 116, 355, 380, 775
Hpy166II GTNNAC 1 cut(s) 637
Hpy188I TCNGA 8 cut(s) 201, 303, 308, 457, 617, 732, 772, 794
Hpy188III TCNNGA 9 cut(s) 157, 179, 290, 292, 506, 581, 740, 899, 935
Hpy8I GTNNAC 1 cut(s) 637
HpyAV CCTTC 3 cut(s) 211, 975, 981
HpyCH4III ACNGT 3 cut(s) 49, 65, 1039
HpyCH4IV ACGT 2 cut(s) 750, 951
HpyCH4V TGCA 9 cut(s) 98, 194, 209, 466, 587, 602, 870, 877, 1053
HpyF10VI GCNNNNNNNGC 1 cut(s) 331
HpyF3I CTNAG 2 cut(s) 200, 554
HpySE526I ACGT 2 cut(s) 750, 951
Hsp92II CATG 6 cut(s) 149, 217, 451, 472, 606, 1035
KflI GGGWCCC 1 cut(s) 559
Kpn2I TCCGGA 1 cut(s) 580
Kzo9I GATC 6 cut(s) 181, 280, 303, 345, 762, 772
LguI GCTCTTC 1 cut(s) 390
Lsp1109I GCAGC 4 cut(s) 41, 82, 312, 452
LweI GCATC 6 cut(s) 181, 218, 241, 343, 574, 798
MabI ACCWGGT 1 cut(s) 861
MaeI CTAG 1 cut(s) 1023
MaeII ACGT 2 cut(s) 750, 951
MaeIII GTNAC 2 cut(s) 43, 235
MalI GATC 6 cut(s) 183, 282, 305, 347, 764, 774
MboI GATC 6 cut(s) 181, 280, 303, 345, 762, 772
MboII GAAGA 5 cut(s) 377, 492, 966, 972, 975
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 8 cut(s) 572, 597, 692, 883, 924, 946, 994, 1092
MmeI TCCRAC 1 cut(s) 1040
Mph1103I ATGCAT 1 cut(s) 211
MroI TCCGGA 1 cut(s) 580
MseI TTAA 3 cut(s) 857, 1058, 1108
MslI CAYNNNNRTG 1 cut(s) 144
MspI CCGG 2 cut(s) 581, 987
MspR9I CCNGG 2 cut(s) 746, 863
MvaI CCWGG 2 cut(s) 746, 863
MwoI GCNNNNNNNGC 1 cut(s) 331
NdeII GATC 6 cut(s) 181, 280, 303, 345, 762, 772
NlaIII CATG 6 cut(s) 149, 217, 451, 472, 606, 1035
NlaIV GGNNCC 4 cut(s) 183, 421, 560, 561
NmuCI GTSAC 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 1 cut(s) 606
PaeI GCATGC 1 cut(s) 606
PaeR7I CTCGAG 1 cut(s) 290
PciSI GCTCTTC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 747
PfeI GAWTC 5 cut(s) 163, 319, 483, 623, 807
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 5 cut(s) 31, 97, 327, 442, 833
Ppu21I YACGTR 1 cut(s) 952
PpuMI RGGWCCY 1 cut(s) 559
Psp5II RGGWCCY 1 cut(s) 559
Psp6I CCWGG 2 cut(s) 744, 861
PspGI CCWGG 2 cut(s) 744, 861
PspN4I GGNNCC 4 cut(s) 183, 421, 560, 561
PspPI GGNCC 6 cut(s) 355, 559, 684, 989, 1026, 1035
PspPPI RGGWCCY 1 cut(s) 559
PstI CTGCAG 1 cut(s) 1055
PsuI RGATCY 1 cut(s) 181
RsaI GTAC 2 cut(s) 67, 710
RsaNI GTAC 2 cut(s) 66, 709
RseI CAYNNNNRTG 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 3 cut(s) 857, 1058, 1108
SatI GCNGC 5 cut(s) 30, 96, 326, 441, 832
Sau3AI GATC 6 cut(s) 181, 280, 303, 345, 762, 772
Sau96I GGNCC 6 cut(s) 355, 559, 684, 989, 1026, 1035
ScaI AGTACT 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 746, 863
SduI GDGCHC 1 cut(s) 756
SexAI ACCWGGT 1 cut(s) 861
SfaNI GCATC 6 cut(s) 181, 218, 241, 343, 574, 798
SfcI CTRYAG 1 cut(s) 1051
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 5 cut(s) 355, 559, 684, 989, 1035
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 144
SmlI CTYRAG 3 cut(s) 290, 414, 715
SmoI CTYRAG 3 cut(s) 290, 414, 715
SphI GCATGC 1 cut(s) 606
Sse9I AATT 8 cut(s) 572, 597, 692, 883, 924, 946, 994, 1092
SsiI CCGC 1 cut(s) 831
SspI AATATT 2 cut(s) 172, 276
SspMI CTAG 1 cut(s) 1023
StyD4I CCNGG 2 cut(s) 744, 861
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 3 cut(s) 49, 65, 1039
TaiI ACGT 2 cut(s) 753, 954
TaqI TCGA 4 cut(s) 279, 291, 507, 900
TasI AATT 8 cut(s) 572, 597, 692, 883, 924, 946, 994, 1092
TatI WGTACW 1 cut(s) 65
TauI GCSGC 1 cut(s) 834
TfiI GAWTC 5 cut(s) 163, 319, 483, 623, 807
Tru1I TTAA 3 cut(s) 857, 1058, 1108
Tru9I TTAA 3 cut(s) 857, 1058, 1108
TscAI CASTG 2 cut(s) 249, 544
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 4 cut(s) 29, 95, 325, 440
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 7 cut(s) 110, 230, 436, 769, 966, 1085, 1104
TspGWI ACGGA 3 cut(s) 548, 697, 765
TspRI CASTG 2 cut(s) 249, 544
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 5 cut(s) 355, 559, 684, 989, 1035
XagI CCTNNNNNAGG 1 cut(s) 651
XapI RAATTY 2 cut(s) 924, 1092
XceI RCATGY 1 cut(s) 606
XhoI CTCGAG 1 cut(s) 290
XspI CTAG 1 cut(s) 1023
ZrmI AGTACT 1 cut(s) 67
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.