FvH4_5g24170

Guanine nucleotide-binding protein subunit beta-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002397
Physical Location & Seq
Forward (+)
15453154 .. 15454352
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24170.t1

Sequence Viewer

Length: 624 bp
ATGGGAGCTGGTGTGGAAGAGAAAGTAGATGGTGCAATGCCAGTGACTACATTGTCGCCACCAGAAGGGGACACAACATTCCTTGATGGCACAACATGGTGTGTGGCTCTTGCTGGTGTCTCCCAAGGGTCTTCTAATGGTTCCTTGTGCGCCAGCAGAGGCAAAGATGGAGTCATTTTGCTGTGGGATTTGACCGAGGGAAAGAGGCTGTACTCGCTTGATGCTGGTGTGATTATTCATGCTCTGTGCTTCAGCCCCAACAAGTATTGGTTGTGCGCTGCGACTGGGCAGAGCATTAAGATCTGGGATTTGGAGAGCAAAAACATTGTTGAGGATTTGAAGACAGAGGCAGATAAGACAAAGAAGACCGGCACTGCCAACAAGAAGAAGGTCCAAAGGGCACCAGCCCCACCCAAGGAATCAAGACCTATCAAGGACCATGTCATAAAGTCTAGAAAGGGACGGAAGAAAATGAAGTTGGCAATGGAAGGTAGTTCTGCTGAAAATGGTAATGCATCAGCTGGCCTCAACACAACCTTTTACTCAAAACAGCCAAAATCATGGAGCAAAAGGTGCTCAAAAGTGAATGTGCTGGCTTTTAAAATAGAGAAACATAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003735 GO:0005078 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006417 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010608 GO:0010646 GO:0010647 GO:0016020 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022613 GO:0022622 GO:0022626 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0032947 GO:0032991 GO:0033993 GO:0034248 GO:0035591 GO:0042221 GO:0042254 GO:0042325 GO:0042327 GO:0042788 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043408 GO:0043410 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045937 GO:0048364 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051704 GO:0051707 GO:0051716 GO:0060090 GO:0060255 GO:0060263 GO:0060267 GO:0065007 GO:0070013 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071840 GO:0071944 GO:0080090 GO:0090351 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1990904 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko05162 map05162
Pfam Domains
Protein Families
WD40

Protein Analysis

208

Amino Acids

22.38

Weight (kDa)

9.51

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 44 - 118 7.7e-08 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 44 - 120 7.7e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 44 - 118 6.5e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 46 - 119 2.1e-09 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 235
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 2 cut(s) 65, 415
AgsI TTSAA 1 cut(s) 340
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 2 cut(s) 8, 521
AluI AGCT 2 cut(s) 8, 521
Alw21I GWGCWC 1 cut(s) 578
Alw26I GTCTC 1 cut(s) 124
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 1 cut(s) 278
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
AspLEI GCGC 2 cut(s) 152, 278
AspS9I GGNCC 2 cut(s) 391, 436
AvaII GGWCC 2 cut(s) 391, 436
BaeGI GKGCMC 1 cut(s) 403
BanI GGYRCC 1 cut(s) 400
BbsI GAAGAC 3 cut(s) 123, 347, 371
Bbv12I GWGCWC 1 cut(s) 578
BbvI GCAGC 1 cut(s) 265
BccI CCATC 3 cut(s) 23, 80, 161
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 1 cut(s) 453
BglII AGATCT 1 cut(s) 300
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
Bme18I GGWCC 2 cut(s) 391, 436
BmgT120I GGNCC 2 cut(s) 391, 436
BmiI GGNNCC 2 cut(s) 142, 402
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 2 cut(s) 211, 524
BmuI ACTGGG 1 cut(s) 294
BpiI GAAGAC 3 cut(s) 123, 347, 371
BsaJI CCNNGG 3 cut(s) 124, 195, 414
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 415
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 2 cut(s) 41, 289
Bse3DI GCAATG 2 cut(s) 42, 489
BseDI CCNNGG 3 cut(s) 124, 195, 414
BseLI CCNNNNNNNGG 2 cut(s) 65, 415
BseMI GCAATG 2 cut(s) 42, 489
BseNI ACTGG 2 cut(s) 41, 289
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 578
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 2 cut(s) 83, 474
BslI CCNNNNNNNGG 2 cut(s) 65, 415
BsmAI GTCTC 1 cut(s) 124
BsmFI GGGAC 2 cut(s) 83, 474
BsnI GGCC 1 cut(s) 525
Bsp1286I GDGCHC 2 cut(s) 403, 578
Bsp143I GATC 1 cut(s) 300
BspANI GGCC 1 cut(s) 525
BspLI GGNNCC 2 cut(s) 142, 402
BspT107I GGYRCC 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 42, 489
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 2 cut(s) 41, 289
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 3 cut(s) 124, 195, 414
BssMI GATC 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 124, 414
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 573
BstC8I GCNNGC 3 cut(s) 154, 523, 594
BstHHI GCGC 2 cut(s) 152, 278
BstKTI GATC 1 cut(s) 303
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 1 cut(s) 300
BstMWI GCNNNNNNNGC 2 cut(s) 214, 573
BstSLI GKGCMC 1 cut(s) 403
BstV1I GCAGC 1 cut(s) 265
BstV2I GAAGAC 3 cut(s) 123, 347, 371
BstX2I RGATCY 1 cut(s) 300
BstXI CCANNNNNNTGG 1 cut(s) 561
BstYI RGATCY 1 cut(s) 300
BsuRI GGCC 1 cut(s) 525
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 2 cut(s) 48, 372
Cac8I GCNNGC 3 cut(s) 154, 523, 594
CfoI GCGC 2 cut(s) 152, 278
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 2 cut(s) 391, 436
Csp6I GTAC 1 cut(s) 211
CviAII CATG 4 cut(s) 96, 239, 440, 561
CviJI RGCY 9 cut(s) 8, 107, 208, 255, 407, 521, 525, 553, 596
CviKI_1 RGCY 9 cut(s) 8, 107, 208, 255, 407, 521, 525, 553, 596
CviQI GTAC 1 cut(s) 211
DpnI GATC 1 cut(s) 302
DpnII GATC 1 cut(s) 300
DraI TTTAAA 1 cut(s) 601
DrdI GACNNNNNNGTC 1 cut(s) 52
DseDI GACNNNNNNGTC 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 2 cut(s) 124, 414
Eco47I GGWCC 2 cut(s) 391, 436
Eco57I CTGAAG 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 124, 414
EcoT22I ATGCAT 1 cut(s) 517
ErhI CCWWGG 2 cut(s) 124, 414
FaeI CATG 4 cut(s) 99, 242, 443, 564
FaiI YATR 6 cut(s) 97, 240, 441, 446, 562, 615
FaqI GGGAC 2 cut(s) 83, 474
FatI CATG 4 cut(s) 95, 238, 439, 560
Fnu4HI GCNGC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 1 cut(s) 453
GlaI GCGC 2 cut(s) 151, 277
GluI GCNGC 1 cut(s) 279
HaeIII GGCC 1 cut(s) 525
HapII CCGG 1 cut(s) 369
HhaI GCGC 2 cut(s) 152, 278
Hin1II CATG 4 cut(s) 99, 242, 443, 564
Hin6I GCGC 2 cut(s) 150, 276
HinP1I GCGC 2 cut(s) 150, 276
HinfI GANTC 2 cut(s) 171, 419
HpaII CCGG 1 cut(s) 369
Hpy188III TCNNGA 2 cut(s) 423, 453
HpyAV CCTTC 3 cut(s) 59, 382, 482
HpyCH4V TGCA 2 cut(s) 35, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 573
Hsp92II CATG 4 cut(s) 99, 242, 443, 564
HspAI GCGC 2 cut(s) 150, 276
Kzo9I GATC 1 cut(s) 300
LmnI GCTCC 2 cut(s) 5, 564
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 2 cut(s) 211, 524
MaeI CTAG 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 302
MboI GATC 1 cut(s) 300
MboII GAAGA 6 cut(s) 29, 123, 352, 376, 397, 478
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 403, 578
MlyI GAGTC 1 cut(s) 180
MnlI CCTC 6 cut(s) 152, 190, 198, 325, 340, 536
Mph1103I ATGCAT 1 cut(s) 517
MseI TTAA 2 cut(s) 297, 600
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 1 cut(s) 369
MwoI GCNNNNNNNGC 2 cut(s) 214, 573
NdeII GATC 1 cut(s) 300
NlaIII CATG 4 cut(s) 99, 242, 443, 564
NlaIV GGNNCC 2 cut(s) 142, 402
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 517
PfeI GAWTC 1 cut(s) 419
PflFI GACNNNGTC 1 cut(s) 440
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 142, 402
PspPI GGNCC 2 cut(s) 391, 436
PsuI RGATCY 1 cut(s) 300
PsyI GACNNNGTC 1 cut(s) 440
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SaqAI TTAA 2 cut(s) 297, 600
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 1 cut(s) 300
Sau96I GGNCC 2 cut(s) 391, 436
SchI GAGTC 1 cut(s) 180
SduI GDGCHC 2 cut(s) 403, 578
SetI ASST 7 cut(s) 10, 393, 430, 493, 523, 539, 575
SfaNI GCATC 2 cut(s) 211, 524
SinI GGWCC 2 cut(s) 391, 436
SspMI CTAG 1 cut(s) 453
StyI CCWWGG 2 cut(s) 124, 414
TaqII GACCGA 1 cut(s) 209
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 2 cut(s) 297, 600
Tru9I TTAA 2 cut(s) 297, 600
TscAI CASTG 2 cut(s) 48, 379
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 227, 488
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 2 cut(s) 48, 379
Tth111I GACNNNGTC 1 cut(s) 440
VpaK11BI GGWCC 2 cut(s) 391, 436
XbaI TCTAGA 1 cut(s) 452
XspI CTAG 1 cut(s) 453
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.