FvH4_6g39960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002397
Physical Location & Seq
Forward (+)
31553633 .. 31555693
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39960.t1

Sequence Viewer

Length: 564 bp
ATGAGATTAGCGATGAGATTAACGATGAGAAACTCGTCGCATTGCTCGAGAGGGGCGCGATTTTATTGGCGAAGAGAGAGGCCGCAAAGAAAGAAGATTAACTTTGGTGGTGGCGGAGGAGAGGATGGTGTACGCAGTGATACTATTCCTCGATCAATTCCTATCAGAGGATCCCTAGTATCAAACAATTACGTTAAAGTAATTGATAACCATGATGGAAAGGGTTGCATTCAAGTACAGATTAAGGATGGTAAAACGGTAGCTGTTAGTGCTAAGGATATCAATGTTGTGATTTTTACCAATTCGGAAATCAAGGCCGTTGTTGTTATTGTTCCTGAATACTTTAATGGTGGTGTTGTGTTTATTAGGTATATTGTCCAAAAGGCACCAGCCCCATCTAAGGAATCAAAACCTATCAAGGACCAGGTCATAAAGTCTAGAAAGGGATGGGAGAAAATGAAGTTGGCAATGGAGGGTAGTTATGCTGGAAATGGTAATGCATCAGCCGGCCTCAACACAACCTCCTACTCAAAGCAGCTAAAATCATGGAGCAAGGGGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

20.67

Weight (kDa)

10.17

Isoelectric Point (pI)

25.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 385
AccII CGCG 1 cut(s) 58
AciI CCGC 2 cut(s) 83, 114
AclWI GGATC 2 cut(s) 165, 178
AfaI GTAC 2 cut(s) 132, 237
AfiI CCNNNNNNNGG 2 cut(s) 167, 400
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 1 cut(s) 423
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AluBI AGCT 2 cut(s) 263, 538
AluI AGCT 2 cut(s) 263, 538
AlwI GGATC 2 cut(s) 165, 178
Ama87I CYCGRG 1 cut(s) 46
AoxI GGCC 3 cut(s) 80, 315, 508
ApeKI GCWGC 1 cut(s) 535
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 46
AvaII GGWCC 1 cut(s) 421
BamHI GGATCC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 385
BbvI GCAGC 1 cut(s) 547
BccI CCATC 5 cut(s) 119, 209, 242, 403, 441
BceAI ACGGC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 425
BfaI CTAG 2 cut(s) 176, 438
BisI GCNGC 2 cut(s) 83, 536
BlsI GCNGC 2 cut(s) 84, 537
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 421
BmiI GGNNCC 2 cut(s) 172, 387
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 506, 536
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 400
Bse118I RCCGGY 1 cut(s) 506
Bse3DI GCAATG 2 cut(s) 40, 474
BseBI CCWGG 1 cut(s) 425
BseGI GGATG 3 cut(s) 130, 253, 452
BseLI CCNNNNNNNGG 2 cut(s) 167, 400
BseMI GCAATG 2 cut(s) 40, 474
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 547
Bsh1236I CGCG 1 cut(s) 58
BshFI GGCC 3 cut(s) 82, 317, 510
BshNI GGYRCC 1 cut(s) 385
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 1 cut(s) 507
BslI CCNNNNNNNGG 2 cut(s) 167, 400
BsmI GAATGC 1 cut(s) 228
BsnI GGCC 3 cut(s) 82, 317, 510
BsoBI CYCGRG 1 cut(s) 46
Bsp143I GATC 2 cut(s) 152, 170
BspACI CCGC 2 cut(s) 83, 114
BspANI GGCC 3 cut(s) 82, 317, 510
BspFNI CGCG 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 172, 387
BspPI GGATC 2 cut(s) 165, 178
BspT107I GGYRCC 1 cut(s) 385
BsrDI GCAATG 2 cut(s) 40, 474
BsrFI RCCGGY 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 506
BssMI GATC 2 cut(s) 152, 170
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 2 cut(s) 273, 399
BstENI CCTNNNNNAGG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 130, 253, 452
BstFNI CGCG 1 cut(s) 58
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 2 cut(s) 155, 173
BstMBI GATC 2 cut(s) 152, 170
BstMWI GCNNNNNNNGC 2 cut(s) 269, 558
BstNI CCWGG 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 423
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 1 cut(s) 547
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
BsuRI GGCC 3 cut(s) 82, 317, 510
BtgZI GCGATG 1 cut(s) 26
BtsCI GGATG 3 cut(s) 130, 253, 452
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 508
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 1 cut(s) 421
CsiI ACCWGGT 1 cut(s) 423
Csp6I GTAC 2 cut(s) 131, 236
CviAII CATG 2 cut(s) 212, 546
CviJI RGCY 7 cut(s) 82, 263, 317, 392, 506, 510, 538
CviKI_1 RGCY 7 cut(s) 82, 263, 317, 392, 506, 510, 538
CviQI GTAC 2 cut(s) 131, 236
DdeI CTNAG 2 cut(s) 273, 399
DpnI GATC 2 cut(s) 154, 172
DpnII GATC 2 cut(s) 152, 170
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 129
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 421
Eco88I CYCGRG 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 423
EcoRV GATATC 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 502
FaeI CATG 2 cut(s) 215, 549
FaiI YATR 5 cut(s) 213, 372, 431, 483, 547
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FatI CATG 2 cut(s) 211, 545
Fnu4HI GCNGC 2 cut(s) 83, 536
FokI GGATG 3 cut(s) 137, 260, 459
Fsp4HI GCNGC 2 cut(s) 83, 536
FspBI CTAG 2 cut(s) 176, 438
GlaI GCGC 1 cut(s) 57
GluI GCNGC 2 cut(s) 83, 536
HaeIII GGCC 3 cut(s) 82, 317, 510
HapII CCGG 1 cut(s) 507
HhaI GCGC 1 cut(s) 58
Hin1II CATG 2 cut(s) 215, 549
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 1 cut(s) 404
HpaII CCGG 1 cut(s) 507
Hpy166II GTNNAC 1 cut(s) 131
Hpy188I TCNGA 2 cut(s) 167, 307
Hpy188III TCNNGA 3 cut(s) 48, 335, 438
Hpy8I GTNNAC 1 cut(s) 131
Hpy99I CGWCG 1 cut(s) 40
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 228, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 558
HpyF3I CTNAG 2 cut(s) 273, 399
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 2 cut(s) 215, 549
HspAI GCGC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 506
KroNI GCCGGC 1 cut(s) 508
Kzo9I GATC 2 cut(s) 152, 170
LmnI GCTCC 1 cut(s) 549
LpnPI CCDG 6 cut(s) 348, 402, 410, 437, 471, 520
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 1 cut(s) 509
MabI ACCWGGT 1 cut(s) 423
MaeI CTAG 2 cut(s) 176, 438
MaeII ACGT 1 cut(s) 192
MalI GATC 2 cut(s) 154, 172
MboI GATC 2 cut(s) 152, 170
MboII GAAGA 2 cut(s) 84, 106
MflI RGATCY 1 cut(s) 170
MluCI AATT 4 cut(s) 156, 187, 201, 301
MnlI CCTC 9 cut(s) 44, 72, 110, 115, 159, 161, 466, 521, 532
Mph1103I ATGCAT 1 cut(s) 502
MroNI GCCGGC 1 cut(s) 506
MseI TTAA 5 cut(s) 20, 99, 195, 243, 345
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 228
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 2 cut(s) 269, 558
NaeI GCCGGC 1 cut(s) 508
NdeII GATC 2 cut(s) 152, 170
NgoMIV GCCGGC 1 cut(s) 506
NlaIII CATG 2 cut(s) 215, 549
NlaIV GGNNCC 2 cut(s) 172, 387
NsiI ATGCAT 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PctI GAATGC 1 cut(s) 228
PdiI GCCGGC 1 cut(s) 508
PfeI GAWTC 1 cut(s) 404
PflFI GACNNNGTC 1 cut(s) 425
PkrI GCNGC 2 cut(s) 84, 537
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 2 cut(s) 172, 387
PspPI GGNCC 1 cut(s) 421
PsuI RGATCY 1 cut(s) 170
PsyI GACNNNGTC 1 cut(s) 425
RsaI GTAC 2 cut(s) 132, 237
RsaNI GTAC 2 cut(s) 131, 236
SaqAI TTAA 5 cut(s) 20, 99, 195, 243, 345
SatI GCNGC 2 cut(s) 83, 536
Sau3AI GATC 2 cut(s) 152, 170
Sau96I GGNCC 1 cut(s) 421
ScrFI CCNGG 1 cut(s) 425
SetI ASST 7 cut(s) 195, 265, 371, 415, 429, 524, 540
SexAI ACCWGGT 1 cut(s) 423
SfaNI GCATC 1 cut(s) 509
Sfr274I CTCGAG 1 cut(s) 46
SinI GGWCC 1 cut(s) 421
SlaI CTCGAG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 4 cut(s) 156, 187, 201, 301
SsiI CCGC 2 cut(s) 83, 114
SspMI CTAG 2 cut(s) 176, 438
StyD4I CCNGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 47, 151
TasI AATT 4 cut(s) 156, 187, 201, 301
TatI WGTACW 1 cut(s) 235
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 5 cut(s) 20, 99, 195, 243, 345
Tru9I TTAA 5 cut(s) 20, 99, 195, 243, 345
TscAI CASTG 1 cut(s) 142
TseI GCWGC 1 cut(s) 535
TspDTI ATGAA 1 cut(s) 473
TspRI CASTG 1 cut(s) 142
Tth111I GACNNNGTC 1 cut(s) 425
VpaK11BI GGWCC 1 cut(s) 421
XagI CCTNNNNNAGG 1 cut(s) 165
XbaI TCTAGA 1 cut(s) 437
XhoI CTCGAG 1 cut(s) 46
XspI CTAG 2 cut(s) 176, 438
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.