FvH4_5g33240
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005624
Physical Location & Seq
Forward (+)
24050928 .. 24051485
558 bp
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UTR
Exon/CDS
Intron
FvH4_5g33240.t1

Sequence Viewer

Length: 558 bp
ATGGAGGAAGTGCATGATGAGCCTGCAGCAAAGTACAGAGGCGTACGCAAGCGAAAATGGGGCAAATGGGTGTCCGAAATCCGGGAGCCGAGAAAGAAAAGCCGGATATGGCTAGGAAGCTACGAGGCCCCAGAAATGGCGGCCGCGGCCTATGATGCGGCTGCATTACACCTCAAAGGACCAGGTGTGGTGCTTAACTTTCCCGAAATGGTTGATAGTCTTCCGAGGCCTGCGAGTTCGAATCCCACCGACGTGCAGTTAGCGGCTCAAGAGGCGGCCAATGCAATAAGGTTTAGGAGTCAGCCTATGAATTCGGTTTCGACGGAGGTGGCTGGTGGATCGAGTGGTGGCGCGGCGCCGGTGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCGCCATTGGACTCGCCTATGATGTGGATGCAGGACATGTACATGTCTTATAGTGACGAGGCTTTGGTTTTTGGGGAGGACATGTCGTCATCTATGGGTTTCTATGATGACCATGAGGATGTTTTGCTGCATCAGTCAATATGGGACTCCTAG

Protein Analysis

186

Amino Acids

20.34

Weight (kDa)

4.79

Isoelectric Point (pI)

65.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 11 - 60 5.7e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 355
AccII CGCG 2 cut(s) 146, 353
AciI CCGC 7 cut(s) 140, 144, 146, 158, 263, 275, 353
AclWI GGATC 1 cut(s) 346
AcoI YGGCCR 2 cut(s) 141, 276
AcsI RAATTY 2 cut(s) 310, 387
AcyI GRCGYC 1 cut(s) 356
AfaI GTAC 3 cut(s) 35, 45, 446
AfiI CCNNNNNNNGG 2 cut(s) 81, 136
AflIII ACRYGT 3 cut(s) 441, 447, 486
AhdI GACNNNNNGTC 1 cut(s) 490
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 181
AleI CACNNNNGTG 1 cut(s) 251
AluBI AGCT 1 cut(s) 120
AluI AGCT 1 cut(s) 120
AlwI GGATC 1 cut(s) 346
AoxI GGCC 5 cut(s) 126, 141, 147, 227, 276
ApeKI GCWGC 3 cut(s) 26, 161, 532
ApoI RAATTY 2 cut(s) 310, 387
AseI ATTAAT 1 cut(s) 399
AspLEI GCGC 2 cut(s) 353, 358
AspS9I GGNCC 2 cut(s) 127, 179
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 373
AsuII TTCGAA 1 cut(s) 239
AvaII GGWCC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 212
BbvI GCAGC 3 cut(s) 38, 148, 519
BciT130I CCWGG 1 cut(s) 183
BcnI CCSGG 1 cut(s) 83
BfaI CTAG 2 cut(s) 113, 556
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 359
Bme1390I CCNGG 2 cut(s) 83, 183
Bme18I GGWCC 1 cut(s) 179
BmeRI GACNNNNNGTC 1 cut(s) 490
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 127, 179
BmiI GGNNCC 3 cut(s) 87, 129, 357
BmrFI CCNGG 2 cut(s) 83, 183
BmsI GCATC 3 cut(s) 145, 423, 544
BpiI GAAGAC 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 2 cut(s) 144, 224
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 136
Bse118I RCCGGY 1 cut(s) 358
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 2 cut(s) 144, 224
BseGI GGATG 2 cut(s) 438, 529
BseLI CCNNNNNNNGG 2 cut(s) 81, 136
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 3 cut(s) 38, 148, 519
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 2 cut(s) 146, 353
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 5 cut(s) 128, 143, 149, 229, 278
BshNI GGYRCC 1 cut(s) 355
BsiEI CGRYCG 1 cut(s) 144
BsiSI CCGG 3 cut(s) 82, 103, 359
BsiWI CGTACG 1 cut(s) 43
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 2 cut(s) 81, 136
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 5 cut(s) 128, 143, 149, 229, 278
Bsp119I TTCGAA 1 cut(s) 239
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 1 cut(s) 338
BspACI CCGC 7 cut(s) 140, 144, 146, 158, 263, 275, 353
BspANI GGCC 5 cut(s) 128, 143, 149, 229, 278
BspFNI CGCG 2 cut(s) 146, 353
BspLI GGNNCC 3 cut(s) 87, 129, 357
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 1 cut(s) 346
BspT104I TTCGAA 1 cut(s) 239
BspT107I GGYRCC 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 358
BsrGI TGTACA 1 cut(s) 444
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 2 cut(s) 144, 224
BssMI GATC 1 cut(s) 338
BssNI GRCGYC 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 367, 375
BstACI GRCGYC 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 444
BstBI TTCGAA 1 cut(s) 239
BstC8I GCNNGC 3 cut(s) 24, 50, 231
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 438, 529
BstFNI CGCG 2 cut(s) 146, 353
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 2 cut(s) 353, 358
BstKTI GATC 1 cut(s) 341
BstMBI GATC 1 cut(s) 338
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 5 cut(s) 19, 146, 155, 272, 281
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 3 cut(s) 445, 451, 490
BstSCI CCNGG 2 cut(s) 81, 181
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 2 cut(s) 146, 353
BstV1I GCAGC 3 cut(s) 38, 148, 519
BstV2I GAAGAC 1 cut(s) 212
BstZI CGGCCG 1 cut(s) 141
BsuRI GGCC 5 cut(s) 128, 143, 149, 229, 278
BtgI CCRYGG 1 cut(s) 144
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 2 cut(s) 438, 529
Cac8I GCNNGC 3 cut(s) 24, 50, 231
CciNI GCGGCCGC 1 cut(s) 141
CfoI GCGC 2 cut(s) 353, 358
Cfr10I RCCGGY 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 127, 179
Cfr42I CCGCGG 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 181
Csp6I GTAC 3 cut(s) 34, 44, 445
CviAII CATG 5 cut(s) 14, 442, 448, 487, 518
CviQI GTAC 3 cut(s) 34, 44, 445
DinI GGCGCC 1 cut(s) 357
DpnI GATC 1 cut(s) 340
DpnII GATC 1 cut(s) 338
DriI GACNNNNNGTC 1 cut(s) 490
EaeI YGGCCR 2 cut(s) 141, 276
EagI CGGCCG 1 cut(s) 141
Eam1105I GACNNNNNGTC 1 cut(s) 490
EclXI CGGCCG 1 cut(s) 141
Eco147I AGGCCT 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 179
Eco52I CGGCCG 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 181
EgeI GGCGCC 1 cut(s) 357
EheI GGCGCC 1 cut(s) 357
FaeI CATG 5 cut(s) 17, 445, 451, 490, 521
FaqI GGGAC 1 cut(s) 384
FatI CATG 5 cut(s) 13, 441, 447, 486, 517
FokI GGATG 2 cut(s) 445, 536
FspBI CTAG 2 cut(s) 113, 556
GlaI GCGC 2 cut(s) 352, 357
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 5 cut(s) 128, 143, 149, 229, 278
HapII CCGG 3 cut(s) 82, 103, 359
HhaI GCGC 2 cut(s) 353, 358
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 5 cut(s) 17, 445, 451, 490, 521
Hin6I GCGC 2 cut(s) 351, 356
HinP1I GCGC 2 cut(s) 351, 356
HinfI GANTC 6 cut(s) 241, 298, 382, 404, 416, 551
HpaII CCGG 3 cut(s) 82, 103, 359
HphI GGTGA 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 76, 225
Hpy188III TCNNGA 2 cut(s) 203, 269
Hpy99I CGWCG 2 cut(s) 254, 325
HpyCH4III ACNGT 2 cut(s) 367, 375
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 7 cut(s) 13, 26, 164, 256, 284, 436, 535
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 146, 155, 272, 281
HpySE526I ACGT 1 cut(s) 252
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 5 cut(s) 17, 445, 451, 490, 521
HspAI GCGC 2 cut(s) 351, 356
KasI GGCGCC 1 cut(s) 355
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 1 cut(s) 338
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 3 cut(s) 38, 148, 519
LweI GCATC 3 cut(s) 145, 423, 544
MabI ACCWGGT 1 cut(s) 181
MaeI CTAG 2 cut(s) 113, 556
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 361, 458
MalI GATC 1 cut(s) 340
MboI GATC 1 cut(s) 338
MboII GAAGA 1 cut(s) 212
MluCI AATT 2 cut(s) 310, 387
Mly113I GGCGCC 1 cut(s) 356
MlyI GAGTC 5 cut(s) 307, 391, 410, 413, 545
MnlI CCTC 9 cut(s) 32, 118, 182, 219, 265, 319, 457, 475, 514
MseI TTAA 2 cut(s) 195, 399
MslI CAYNNNNRTG 3 cut(s) 251, 446, 522
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 3 cut(s) 82, 103, 359
MspR9I CCNGG 2 cut(s) 83, 183
MteI GCGCNGCGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 183
MvnI CGCG 2 cut(s) 146, 353
MwoI GCNNNNNNNGC 5 cut(s) 19, 146, 155, 272, 281
NarI GGCGCC 1 cut(s) 356
NciI CCSGG 1 cut(s) 83
NdeII GATC 1 cut(s) 338
NlaIII CATG 5 cut(s) 17, 445, 451, 490, 521
NlaIV GGNNCC 3 cut(s) 87, 129, 357
NmeAIII GCCGAG 2 cut(s) 114, 405
NmuCI GTSAC 2 cut(s) 361, 458
NotI GCGGCCGC 1 cut(s) 141
NspI RCATGY 3 cut(s) 445, 451, 490
NspV TTCGAA 1 cut(s) 239
OliI CACNNNNGTG 1 cut(s) 251
PceI AGGCCT 1 cut(s) 229
PciI ACATGT 3 cut(s) 441, 447, 486
PfeI GAWTC 1 cut(s) 241
Pfl23II CGTACG 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 81
PleI GAGTC 5 cut(s) 306, 390, 410, 412, 545
PluTI GGCGCC 1 cut(s) 359
PpsI GAGTC 5 cut(s) 306, 390, 410, 412, 545
PscI ACATGT 3 cut(s) 441, 447, 486
PshBI ATTAAT 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspLI CGTACG 1 cut(s) 43
PspN4I GGNNCC 3 cut(s) 87, 129, 357
PspPI GGNCC 2 cut(s) 127, 179
PstI CTGCAG 1 cut(s) 28
RsaI GTAC 3 cut(s) 35, 45, 446
RsaNI GTAC 3 cut(s) 34, 44, 445
RseI CAYNNNNRTG 3 cut(s) 251, 446, 522
SacII CCGCGG 1 cut(s) 147
SaqAI TTAA 2 cut(s) 195, 399
Sau3AI GATC 1 cut(s) 338
Sau96I GGNCC 2 cut(s) 127, 179
SchI GAGTC 5 cut(s) 307, 391, 410, 413, 545
ScrFI CCNGG 2 cut(s) 83, 183
SetI ASST 6 cut(s) 122, 174, 187, 255, 293, 330
SexAI ACCWGGT 1 cut(s) 181
SfaNI GCATC 3 cut(s) 145, 423, 544
SfcI CTRYAG 1 cut(s) 24
SfoI GGCGCC 1 cut(s) 357
Sfr303I CCGCGG 1 cut(s) 147
SfuI TTCGAA 1 cut(s) 239
SgrAI CRCCGGYG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 147
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 3 cut(s) 251, 446, 522
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
Sse9I AATT 2 cut(s) 310, 387
SseBI AGGCCT 1 cut(s) 229
SsiI CCGC 7 cut(s) 140, 144, 146, 158, 263, 275, 353
SspDI GGCGCC 1 cut(s) 355
SspMI CTAG 2 cut(s) 113, 556
StuI AGGCCT 1 cut(s) 229
StyD4I CCNGG 2 cut(s) 81, 181
TaaI ACNGT 2 cut(s) 367, 375
TaiI ACGT 1 cut(s) 255
TaqI TCGA 3 cut(s) 239, 320, 341
TasI AATT 2 cut(s) 310, 387
TatI WGTACW 2 cut(s) 33, 444
TauI GCSGC 7 cut(s) 143, 146, 149, 161, 266, 278, 356
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 2 cut(s) 195, 399
Tru9I TTAA 2 cut(s) 195, 399
TseFI GTSAC 2 cut(s) 361, 458
TseI GCWGC 3 cut(s) 26, 161, 532
Tsp45I GTSAC 2 cut(s) 361, 458
TspDTI ATGAA 1 cut(s) 323
TspGWI ACGGA 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 179
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 2 cut(s) 310, 387
XceI RCATGY 3 cut(s) 445, 451, 490
XspI CTAG 2 cut(s) 113, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.