RchiOBHm_Chr7g0231921
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005624
Physical Location & Seq
Forward (+)
55763198 .. 55763832
635 bp
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UTR
Exon/CDS
Intron
PRQ20785

Sequence Viewer

Length: 585 bp
ATGACCCGAGAAACTAATCAAGCCCTCCCGGCAGATATGAAGGAAGCACACGAGCCTGCAGCCAAGTACAGAGGAGTACGCAAGCGAAAATGGGGCAAATGGGTGTCTGAAATCCGCGAGCCAGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCCGAAATGGCGGCCGCGGCCTACGATGCTGCGGCATTGCACCTCAGAGGACCAGGGGTGATGCTTAACTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCCTCGGACGTGCAGTTGGCGGCTCAAGAGGCGACAATGAGGTTTAGGAGACTGCCAATGGGGTCGGTTTCGACTGAGGTGGCTGGTGGCTCGAGTGGTGGCGCGGCGCCGGTGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCGCCATTGGACTCGCCTAAGATGTGGATGCAGTACATGTACATGTCTCATCGTGATGAGGCTTTTTTTGTTCAGGAAGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGATGGTTTGCAGCATCAGTCCATTTGGGACTCCTAG

Protein Analysis

194

Amino Acids

21.48

Weight (kDa)

5.54

Isoelectric Point (pI)

70.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 22 - 71 2e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccII CGCG 3 cut(s) 117, 179, 380
AciI CCGC 7 cut(s) 115, 173, 177, 179, 194, 296, 380
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 414
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 4 cut(s) 68, 78, 467, 473
AfiI CCNNNNNNNGG 2 cut(s) 141, 169
AflIII ACRYGT 2 cut(s) 468, 474
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 2 cut(s) 121, 214
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 319, 483
Ama87I CYCGRG 2 cut(s) 6, 367
AoxI GGCC 4 cut(s) 159, 174, 180, 260
ApeKI GCWGC 3 cut(s) 59, 191, 559
ApoI RAATTY 1 cut(s) 414
AseI ATTAAT 1 cut(s) 426
AspLEI GCGC 2 cut(s) 380, 385
AspS9I GGNCC 2 cut(s) 160, 212
AsuC2I CCSGG 2 cut(s) 29, 136
AsuHPI GGTGA 2 cut(s) 232, 400
AvaI CYCGRG 2 cut(s) 6, 367
AvaII GGWCC 1 cut(s) 212
BanI GGYRCC 1 cut(s) 382
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 2 cut(s) 245, 516
BbvI GCAGC 3 cut(s) 71, 178, 571
BccI CCATC 2 cut(s) 526, 545
BciT130I CCWGG 2 cut(s) 123, 216
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 2 cut(s) 29, 136
BcoDI GTCTC 2 cut(s) 319, 483
BfaI CTAG 2 cut(s) 146, 583
BfmI CTRYAG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 386
BglI GCCNNNNNGGC 1 cut(s) 29
BisI GCNGC 9 cut(s) 60, 174, 177, 180, 192, 195, 297, 381, 560
BlsI GCNGC 9 cut(s) 61, 175, 178, 181, 193, 196, 298, 382, 561
Bme1390I CCNGG 4 cut(s) 29, 123, 136, 216
Bme18I GGWCC 1 cut(s) 212
BmeT110I CYCGRG 2 cut(s) 6, 367
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 160, 212
BmiI GGNNCC 2 cut(s) 162, 384
BmrFI CCNGG 4 cut(s) 29, 123, 136, 216
BmsI GCATC 4 cut(s) 178, 213, 450, 571
BpiI GAAGAC 2 cut(s) 245, 516
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 29, 136
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 4 cut(s) 177, 215, 257, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 169
Bse118I RCCGGY 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 197
BseBI CCWGG 2 cut(s) 123, 216
BseDI CCNNGG 4 cut(s) 177, 215, 257, 279
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 2 cut(s) 141, 169
BseMI GCAATG 1 cut(s) 197
BseMII CTCAG 2 cut(s) 220, 342
BseRI GAGGAG 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 174
BseXI GCAGC 3 cut(s) 71, 178, 571
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 3 cut(s) 117, 179, 380
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 4 cut(s) 161, 176, 182, 262
BshNI GGYRCC 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 177
BsiHKCI CYCGRG 2 cut(s) 6, 367
BsiSI CCGG 3 cut(s) 29, 136, 386
BslFI GGGAC 2 cut(s) 261, 411
BslI CCNNNNNNNGG 2 cut(s) 141, 169
BsmAI GTCTC 2 cut(s) 319, 483
BsmFI GGGAC 2 cut(s) 261, 411
BsnI GGCC 4 cut(s) 161, 176, 182, 262
BsoBI CYCGRG 2 cut(s) 6, 367
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 1 cut(s) 541
BspACI CCGC 7 cut(s) 115, 173, 177, 179, 194, 296, 380
BspANI GGCC 4 cut(s) 161, 176, 182, 262
BspCNI CTCAG 2 cut(s) 219, 343
BspFNI CGCG 3 cut(s) 117, 179, 380
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 2 cut(s) 162, 384
BspMAI CTGCAG 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 385
BsrGI TGTACA 1 cut(s) 471
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 4 cut(s) 177, 215, 257, 279
BssMI GATC 1 cut(s) 541
BssNI GRCGYC 1 cut(s) 383
BssSI CACGAG 1 cut(s) 50
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 123, 216
Bst4CI ACNGT 2 cut(s) 394, 402
BstACI GRCGYC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 471
BstC8I GCNNGC 4 cut(s) 57, 83, 119, 264
BstDEI CTNAG 3 cut(s) 206, 351, 450
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 3 cut(s) 117, 179, 380
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 2 cut(s) 380, 385
BstKTI GATC 1 cut(s) 544
BstMAI GTCTC 2 cut(s) 319, 483
BstMBI GATC 1 cut(s) 541
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 4 cut(s) 29, 179, 188, 305
BstNI CCWGG 2 cut(s) 123, 216
BstNSI RCATGY 3 cut(s) 472, 478, 517
BstSCI CCNGG 4 cut(s) 27, 121, 134, 214
BstSFI CTRYAG 1 cut(s) 57
BstUI CGCG 3 cut(s) 117, 179, 380
BstV1I GCAGC 3 cut(s) 71, 178, 571
BstV2I GAAGAC 2 cut(s) 245, 516
BstZI CGGCCG 1 cut(s) 174
BsuRI GGCC 4 cut(s) 161, 176, 182, 262
BtgI CCRYGG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 1 cut(s) 465
Cac8I GCNNGC 4 cut(s) 57, 83, 119, 264
CciI TCATGA 1 cut(s) 544
CciNI GCGGCCGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 380, 385
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 160, 212
Cfr42I CCGCGG 1 cut(s) 180
Csp6I GTAC 4 cut(s) 67, 77, 466, 472
CviAII CATG 4 cut(s) 469, 475, 514, 545
CviQI GTAC 4 cut(s) 67, 77, 466, 472
DdeI CTNAG 3 cut(s) 206, 351, 450
DinI GGCGCC 1 cut(s) 384
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
EaeI YGGCCR 1 cut(s) 174
EagI CGGCCG 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 212
Eco52I CGGCCG 1 cut(s) 174
Eco88I CYCGRG 2 cut(s) 6, 367
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 121, 214
EgeI GGCGCC 1 cut(s) 384
EheI GGCGCC 1 cut(s) 384
FaeI CATG 4 cut(s) 472, 478, 517, 548
FaiI YATR 8 cut(s) 38, 142, 470, 476, 515, 527, 537, 546
FaqI GGGAC 2 cut(s) 261, 411
FatI CATG 4 cut(s) 468, 474, 513, 544
FbaI TGATCA 1 cut(s) 541
Fnu4HI GCNGC 9 cut(s) 60, 174, 177, 180, 192, 195, 297, 381, 560
FokI GGATG 1 cut(s) 472
Fsp4HI GCNGC 9 cut(s) 60, 174, 177, 180, 192, 195, 297, 381, 560
FspBI CTAG 2 cut(s) 146, 583
GlaI GCGC 2 cut(s) 379, 384
GluI GCNGC 9 cut(s) 60, 174, 177, 180, 192, 195, 297, 381, 560
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 4 cut(s) 161, 176, 182, 262
HapII CCGG 3 cut(s) 29, 136, 386
HhaI GCGC 2 cut(s) 380, 385
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 4 cut(s) 472, 478, 517, 548
Hin6I GCGC 2 cut(s) 378, 383
HinP1I GCGC 2 cut(s) 378, 383
HinfI GANTC 5 cut(s) 274, 409, 431, 443, 578
HpaII CCGG 3 cut(s) 29, 136, 386
HphI GGTGA 2 cut(s) 232, 400
Hpy188I TCNGA 4 cut(s) 109, 209, 258, 283
Hpy188III TCNNGA 5 cut(s) 236, 302, 485, 506, 545
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 2 cut(s) 394, 402
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 5 cut(s) 59, 202, 289, 463, 559
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 179, 188, 305
HpyF3I CTNAG 3 cut(s) 206, 351, 450
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 4 cut(s) 472, 478, 517, 548
HspAI GCGC 2 cut(s) 378, 383
KasI GGCGCC 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 541
KspI CCGCGG 1 cut(s) 180
Kzo9I GATC 1 cut(s) 541
Lsp1109I GCAGC 3 cut(s) 71, 178, 571
LweI GCATC 4 cut(s) 178, 213, 450, 571
MaeI CTAG 2 cut(s) 146, 583
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 388
MalI GATC 1 cut(s) 543
MboI GATC 1 cut(s) 541
MboII GAAGA 2 cut(s) 245, 521
MluCI AATT 1 cut(s) 414
Mly113I GGCGCC 1 cut(s) 383
MlyI GAGTC 5 cut(s) 283, 418, 437, 440, 572
MseI TTAA 2 cut(s) 228, 426
MslI CAYNNNNRTG 3 cut(s) 473, 486, 549
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 3 cut(s) 29, 136, 386
MspR9I CCNGG 4 cut(s) 29, 123, 136, 216
MteI GCGCNGCGC 1 cut(s) 381
MvaI CCWGG 2 cut(s) 123, 216
MvnI CGCG 3 cut(s) 117, 179, 380
MwoI GCNNNNNNNGC 4 cut(s) 29, 179, 188, 305
NarI GGCGCC 1 cut(s) 383
NciI CCSGG 2 cut(s) 29, 136
NdeII GATC 1 cut(s) 541
NlaIII CATG 4 cut(s) 472, 478, 517, 548
NlaIV GGNNCC 2 cut(s) 162, 384
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 388
NotI GCGGCCGC 1 cut(s) 174
NspI RCATGY 3 cut(s) 472, 478, 517
PaeR7I CTCGAG 1 cut(s) 367
PagI TCATGA 1 cut(s) 544
PciI ACATGT 2 cut(s) 468, 474
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PkrI GCNGC 9 cut(s) 61, 175, 178, 181, 193, 196, 298, 382, 561
PleI GAGTC 5 cut(s) 282, 417, 437, 439, 572
PluTI GGCGCC 1 cut(s) 386
PpsI GAGTC 5 cut(s) 282, 417, 437, 439, 572
PscI ACATGT 2 cut(s) 468, 474
PshBI ATTAAT 1 cut(s) 426
Psp6I CCWGG 2 cut(s) 121, 214
PspGI CCWGG 2 cut(s) 121, 214
PspN4I GGNNCC 2 cut(s) 162, 384
PspPI GGNCC 2 cut(s) 160, 212
PspXI VCTCGAGB 1 cut(s) 367
PstI CTGCAG 1 cut(s) 61
RsaI GTAC 4 cut(s) 68, 78, 467, 473
RsaNI GTAC 4 cut(s) 67, 77, 466, 472
RseI CAYNNNNRTG 3 cut(s) 473, 486, 549
SacII CCGCGG 1 cut(s) 180
SaqAI TTAA 2 cut(s) 228, 426
SatI GCNGC 9 cut(s) 60, 174, 177, 180, 192, 195, 297, 381, 560
Sau3AI GATC 1 cut(s) 541
Sau96I GGNCC 2 cut(s) 160, 212
SchI GAGTC 5 cut(s) 283, 418, 437, 440, 572
ScrFI CCNGG 4 cut(s) 29, 123, 136, 216
SetI ASST 5 cut(s) 155, 207, 288, 320, 357
SfaNI GCATC 4 cut(s) 178, 213, 450, 571
SfcI CTRYAG 1 cut(s) 57
SfoI GGCGCC 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 367
Sfr303I CCGCGG 1 cut(s) 180
SgrAI CRCCGGYG 1 cut(s) 385
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 367
SmiMI CAYNNNNRTG 3 cut(s) 473, 486, 549
SmlI CTYRAG 2 cut(s) 300, 367
SmoI CTYRAG 2 cut(s) 300, 367
Sse9I AATT 1 cut(s) 414
SsiI CCGC 7 cut(s) 115, 173, 177, 179, 194, 296, 380
SspDI GGCGCC 1 cut(s) 382
SspMI CTAG 2 cut(s) 146, 583
StyD4I CCNGG 4 cut(s) 27, 121, 134, 214
TaaI ACNGT 2 cut(s) 394, 402
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 272, 347, 368
TasI AATT 1 cut(s) 414
TatI WGTACW 3 cut(s) 66, 465, 471
TauI GCSGC 6 cut(s) 176, 179, 182, 197, 299, 383
Tru1I TTAA 2 cut(s) 228, 426
Tru9I TTAA 2 cut(s) 228, 426
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 3 cut(s) 59, 191, 559
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 212
VspI ATTAAT 1 cut(s) 426
XapI RAATTY 1 cut(s) 414
XceI RCATGY 3 cut(s) 472, 478, 517
XhoI CTCGAG 1 cut(s) 367
XspI CTAG 2 cut(s) 146, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.