Rh7AG422500
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005624
Physical Location & Seq
Forward (+)
56858808 .. 56859900
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG422500.1

Sequence Viewer

Length: 585 bp
ATGACCCAAGAAATTAATCAAACCCTCCGGGCAGATATGGAGGAAGTACACGAGCCTGCAGTCAAGTACAGAGGCGTACGCAAGCGAAAATGGGGTAAATGGGTGTCCGAAATCAGGGAACCGGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCCGAAATGGCCGCCGCCGCCTACGATGCGGCGGCATTGCACCTCAGAGGACCGGGGGTCCTGCTTAATTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCCTCGGACGTGCAGTTGGCGGCTCAAGAGGCGGCTATGAGGTTTAGGAGACTGCCAATGGGGTCGGTTTCGACGGAGGTGGCTGGTGGCTCCAGTGGTGGCGCGGCGCCGGTGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCGCCATTAGACTCACCTAAGATGTGGATGCAGTACATGTACATGTCTCATCGTGATGAGGCTTTGTTTTTTGGGGAGGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGATGGTTTGCAGCATCAGTCCATTTGGGACTCCTAG

Protein Analysis

194

Amino Acids

21.5

Weight (kDa)

5.23

Isoelectric Point (pI)

71.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 22 - 71 1.3e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccII CGCG 1 cut(s) 380
AciI CCGC 8 cut(s) 174, 177, 180, 191, 194, 296, 308, 380
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 414
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 5 cut(s) 48, 68, 78, 467, 473
AfiI CCNNNNNNNGG 3 cut(s) 114, 141, 169
AflIII ACRYGT 2 cut(s) 468, 474
AhdI GACNNNNNGTC 1 cut(s) 218
AjiI CACGTC 1 cut(s) 286
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 319, 483
AoxI GGCC 3 cut(s) 159, 171, 260
ApeKI GCWGC 1 cut(s) 559
ApoI RAATTY 1 cut(s) 414
AseI ATTAAT 2 cut(s) 15, 426
AspLEI GCGC 2 cut(s) 380, 385
AspS9I GGNCC 3 cut(s) 160, 212, 220
AsuC2I CCSGG 4 cut(s) 29, 123, 136, 216
AsuHPI GGTGA 2 cut(s) 400, 438
AvaII GGWCC 2 cut(s) 212, 220
BanI GGYRCC 1 cut(s) 382
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 571
BccI CCATC 2 cut(s) 526, 545
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 4 cut(s) 29, 123, 136, 216
BcoDI GTCTC 2 cut(s) 319, 483
BfaI CTAG 2 cut(s) 146, 583
BfmI CTRYAG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 386
BisI GCNGC 9 cut(s) 174, 177, 180, 192, 195, 297, 309, 381, 560
BlsI GCNGC 9 cut(s) 175, 178, 181, 193, 196, 298, 310, 382, 561
Bme1390I CCNGG 4 cut(s) 29, 123, 136, 216
Bme18I GGWCC 2 cut(s) 212, 220
BmeRI GACNNNNNGTC 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 160, 212, 220
BmiI GGNNCC 5 cut(s) 120, 162, 221, 367, 384
BmrFI CCNGG 4 cut(s) 29, 123, 136, 216
BmsI GCATC 3 cut(s) 178, 450, 571
BpiI GAAGAC 1 cut(s) 245
BpmI CTGGAG 1 cut(s) 352
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 4 cut(s) 29, 123, 136, 216
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 215, 257, 279
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 141, 169
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 369
Bse3DI GCAATG 1 cut(s) 197
BseDI CCNNGG 3 cut(s) 215, 257, 279
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 3 cut(s) 114, 141, 169
BseMI GCAATG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 369
BseXI GCAGC 1 cut(s) 571
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 3 cut(s) 161, 173, 262
BshNI GGYRCC 1 cut(s) 382
BsiSI CCGG 5 cut(s) 28, 122, 136, 215, 386
BsiWI CGTACG 1 cut(s) 76
BslFI GGGAC 2 cut(s) 261, 411
BslI CCNNNNNNNGG 3 cut(s) 114, 141, 169
BsmAI GTCTC 2 cut(s) 319, 483
BsmFI GGGAC 2 cut(s) 261, 411
BsnI GGCC 3 cut(s) 161, 173, 262
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 1 cut(s) 541
BspACI CCGC 8 cut(s) 174, 177, 180, 191, 194, 296, 308, 380
BspANI GGCC 3 cut(s) 161, 173, 262
BspCNI CTCAG 1 cut(s) 219
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 5 cut(s) 120, 162, 221, 367, 384
BspMAI CTGCAG 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 385
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 1 cut(s) 369
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 215, 257, 279
BssMI GATC 1 cut(s) 541
BssNI GRCGYC 1 cut(s) 383
BssSI CACGAG 1 cut(s) 50
Bst2BI CACGAG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 394, 402
BstACI GRCGYC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 57, 83, 264
BstDEI CTNAG 2 cut(s) 206, 450
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 1 cut(s) 380
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 2 cut(s) 380, 385
BstKTI GATC 1 cut(s) 544
BstMAI GTCTC 2 cut(s) 319, 483
BstMBI GATC 1 cut(s) 541
BstMWI GCNNNNNNNGC 3 cut(s) 179, 188, 305
BstNSI RCATGY 3 cut(s) 472, 478, 517
BstSCI CCNGG 4 cut(s) 27, 121, 134, 214
BstSFI CTRYAG 1 cut(s) 57
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 571
BstV2I GAAGAC 1 cut(s) 245
BsuRI GGCC 3 cut(s) 161, 173, 262
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 376
Cac8I GCNNGC 3 cut(s) 57, 83, 264
CciI TCATGA 1 cut(s) 544
CfoI GCGC 2 cut(s) 380, 385
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 160, 212, 220
Csp6I GTAC 5 cut(s) 47, 67, 77, 466, 472
CviAII CATG 4 cut(s) 469, 475, 514, 545
CviQI GTAC 5 cut(s) 47, 67, 77, 466, 472
DdeI CTNAG 2 cut(s) 206, 450
DinI GGCGCC 1 cut(s) 384
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
DriI GACNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 1 cut(s) 171
Eam1105I GACNNNNNGTC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 212, 220
EcoO109I RGGNCCY 2 cut(s) 160, 220
EgeI GGCGCC 1 cut(s) 384
EheI GGCGCC 1 cut(s) 384
FaeI CATG 4 cut(s) 472, 478, 517, 548
FaiI YATR 9 cut(s) 38, 142, 314, 470, 476, 515, 527, 537, 546
FaqI GGGAC 2 cut(s) 261, 411
FatI CATG 4 cut(s) 468, 474, 513, 544
FbaI TGATCA 1 cut(s) 541
Fnu4HI GCNGC 9 cut(s) 174, 177, 180, 192, 195, 297, 309, 381, 560
FokI GGATG 1 cut(s) 472
Fsp4HI GCNGC 9 cut(s) 174, 177, 180, 192, 195, 297, 309, 381, 560
FspBI CTAG 2 cut(s) 146, 583
GlaI GCGC 2 cut(s) 379, 384
GluI GCNGC 9 cut(s) 174, 177, 180, 192, 195, 297, 309, 381, 560
GsuI CTGGAG 1 cut(s) 352
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 3 cut(s) 161, 173, 262
HapII CCGG 5 cut(s) 28, 122, 136, 215, 386
HhaI GCGC 2 cut(s) 380, 385
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 4 cut(s) 472, 478, 517, 548
Hin6I GCGC 2 cut(s) 378, 383
HinP1I GCGC 2 cut(s) 378, 383
HinfI GANTC 5 cut(s) 274, 409, 431, 443, 578
HpaII CCGG 5 cut(s) 28, 122, 136, 215, 386
HphI GGTGA 2 cut(s) 400, 438
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 4 cut(s) 109, 209, 258, 283
Hpy188III TCNNGA 4 cut(s) 236, 302, 485, 545
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 1 cut(s) 352
HpyCH4III ACNGT 2 cut(s) 394, 402
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 5 cut(s) 59, 202, 289, 463, 559
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 188, 305
HpyF3I CTNAG 2 cut(s) 206, 450
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 4 cut(s) 472, 478, 517, 548
HspAI GCGC 2 cut(s) 378, 383
KasI GGCGCC 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 1 cut(s) 541
LmnI GCTCC 1 cut(s) 371
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 3 cut(s) 178, 450, 571
MaeI CTAG 2 cut(s) 146, 583
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 388
MalI GATC 1 cut(s) 543
MboI GATC 1 cut(s) 541
MboII GAAGA 1 cut(s) 245
MluCI AATT 3 cut(s) 12, 229, 414
Mly113I GGCGCC 1 cut(s) 383
MlyI GAGTC 5 cut(s) 283, 418, 437, 440, 572
MseI TTAA 3 cut(s) 15, 228, 426
MslI CAYNNNNRTG 3 cut(s) 473, 486, 549
MspI CCGG 5 cut(s) 28, 122, 136, 215, 386
MspR9I CCNGG 4 cut(s) 29, 123, 136, 216
MteI GCGCNGCGC 1 cut(s) 381
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 3 cut(s) 179, 188, 305
NarI GGCGCC 1 cut(s) 383
NciI CCSGG 4 cut(s) 29, 123, 136, 216
NdeII GATC 1 cut(s) 541
NlaIII CATG 4 cut(s) 472, 478, 517, 548
NlaIV GGNNCC 5 cut(s) 120, 162, 221, 367, 384
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 3 cut(s) 472, 478, 517
PagI TCATGA 1 cut(s) 544
PciI ACATGT 2 cut(s) 468, 474
PcsI WCGNNNNNNNCGW 1 cut(s) 162
Pfl23II CGTACG 1 cut(s) 76
PkrI GCNGC 9 cut(s) 175, 178, 181, 193, 196, 298, 310, 382, 561
PleI GAGTC 5 cut(s) 282, 417, 437, 439, 572
PluTI GGCGCC 1 cut(s) 386
PpsI GAGTC 5 cut(s) 282, 417, 437, 439, 572
PpuMI RGGWCCY 1 cut(s) 220
PscI ACATGT 2 cut(s) 468, 474
PshBI ATTAAT 2 cut(s) 15, 426
Psp5II RGGWCCY 1 cut(s) 220
PspLI CGTACG 1 cut(s) 76
PspN4I GGNNCC 5 cut(s) 120, 162, 221, 367, 384
PspPI GGNCC 3 cut(s) 160, 212, 220
PspPPI RGGWCCY 1 cut(s) 220
PstI CTGCAG 1 cut(s) 61
RsaI GTAC 5 cut(s) 48, 68, 78, 467, 473
RsaNI GTAC 5 cut(s) 47, 67, 77, 466, 472
RseI CAYNNNNRTG 3 cut(s) 473, 486, 549
SaqAI TTAA 3 cut(s) 15, 228, 426
SatI GCNGC 9 cut(s) 174, 177, 180, 192, 195, 297, 309, 381, 560
Sau3AI GATC 1 cut(s) 541
Sau96I GGNCC 3 cut(s) 160, 212, 220
SchI GAGTC 5 cut(s) 283, 418, 437, 440, 572
ScrFI CCNGG 4 cut(s) 29, 123, 136, 216
SetI ASST 6 cut(s) 155, 207, 288, 320, 357, 451
SfaNI GCATC 3 cut(s) 178, 450, 571
SfcI CTRYAG 1 cut(s) 57
SfoI GGCGCC 1 cut(s) 384
SgrAI CRCCGGYG 1 cut(s) 385
SinI GGWCC 2 cut(s) 212, 220
SmiMI CAYNNNNRTG 3 cut(s) 473, 486, 549
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse9I AATT 3 cut(s) 12, 229, 414
SsiI CCGC 8 cut(s) 174, 177, 180, 191, 194, 296, 308, 380
SspDI GGCGCC 1 cut(s) 382
SspMI CTAG 2 cut(s) 146, 583
StyD4I CCNGG 4 cut(s) 27, 121, 134, 214
TaaI ACNGT 2 cut(s) 394, 402
TaiI ACGT 1 cut(s) 288
TaqI TCGA 2 cut(s) 272, 347
TasI AATT 3 cut(s) 12, 229, 414
TatI WGTACW 4 cut(s) 46, 66, 465, 471
TauI GCSGC 8 cut(s) 176, 179, 182, 194, 197, 299, 311, 383
Tru1I TTAA 3 cut(s) 15, 228, 426
Tru9I TTAA 3 cut(s) 15, 228, 426
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 1 cut(s) 559
Tsp45I GTSAC 1 cut(s) 388
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 2 cut(s) 212, 220
VspI ATTAAT 2 cut(s) 15, 426
XapI RAATTY 1 cut(s) 414
XceI RCATGY 3 cut(s) 472, 478, 517
XspI CTAG 2 cut(s) 146, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.