Rh7BG396800
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005624
Physical Location & Seq
Forward (+)
43913936 .. 43914472
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG396800.1

Sequence Viewer

Length: 537 bp
ATGGAGGAAGTACACGAGCCTGCGCCCAAGTACAGAGGCGTACGCAAACGAAAATGGGGTAAATGGGTGTCCGAAATCCGGGAACCGGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCTGAAATGGCGGCCGCTGCCTACGATGCGGCTGCGTTGCACCTCAGAGGACCAGGGGTGTTGCTTAACTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCCTCGGACGTGCAGTTGGCGGCTCAAGAGGCGGCAATGAGGTTTAGGAGACTGCCGATGGGGTCGGTTTCGACGGAGGTGGCTGGTGGCGCGGTCCCGGAGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCACCATTGGACTCGCCTAAGATGTGGATGCAGTACATGTACATGTCTCATCGTGATGAGGCTTTGTTTTTTGGGGAGGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGATGGTTTGCAGCATCAGTCCATTTGGGACTCCTAG

Protein Analysis

178

Amino Acids

19.87

Weight (kDa)

5.22

Isoelectric Point (pI)

75.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 9 - 59 2.9e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 332
AciI CCGC 6 cut(s) 137, 141, 155, 260, 272, 332
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 1 cut(s) 366
AfaI GTAC 5 cut(s) 12, 32, 42, 419, 425
AfiI CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 343
AflIII ACRYGT 2 cut(s) 420, 426
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 178
AluBI AGCT 1 cut(s) 117
AluI AGCT 1 cut(s) 117
Alw26I GTCTC 3 cut(s) 283, 335, 435
AoxI GGCC 3 cut(s) 123, 138, 224
ApeKI GCWGC 3 cut(s) 143, 158, 511
ApoI RAATTY 1 cut(s) 366
AseI ATTAAT 1 cut(s) 378
AspLEI GCGC 2 cut(s) 25, 332
AspS9I GGNCC 3 cut(s) 124, 176, 334
AsuC2I CCSGG 4 cut(s) 80, 87, 100, 338
AsuHPI GGTGA 1 cut(s) 378
AvaII GGWCC 2 cut(s) 176, 334
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 3 cut(s) 130, 145, 523
BccI CCATC 3 cut(s) 292, 478, 497
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 1 cut(s) 180
BclI TGATCA 1 cut(s) 493
BcnI CCSGG 4 cut(s) 80, 87, 100, 338
BcoDI GTCTC 3 cut(s) 283, 335, 435
BfaI CTAG 2 cut(s) 110, 535
BisI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 512
BlsI GCNGC 8 cut(s) 139, 142, 145, 157, 160, 262, 274, 513
Bme1390I CCNGG 5 cut(s) 80, 87, 100, 180, 338
Bme18I GGWCC 2 cut(s) 176, 334
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 3 cut(s) 124, 176, 334
BmiI GGNNCC 3 cut(s) 84, 126, 336
BmrFI CCNGG 5 cut(s) 80, 87, 100, 180, 338
BmsI GCATC 3 cut(s) 142, 402, 523
BpiI GAAGAC 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 249
BpuMI CCSGG 4 cut(s) 80, 87, 100, 338
BsaJI CCNNGG 3 cut(s) 179, 221, 243
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 343
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 3 cut(s) 179, 221, 243
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 343
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 184
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 3 cut(s) 130, 145, 523
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 332
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 125, 140, 226
BsiEI CGRYCG 1 cut(s) 141
BsiSI CCGG 4 cut(s) 79, 86, 100, 338
BsiWI CGTACG 1 cut(s) 40
BslFI GGGAC 3 cut(s) 225, 320, 363
BslI CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 343
BsmAI GTCTC 3 cut(s) 283, 335, 435
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 3 cut(s) 225, 320, 363
BsnI GGCC 3 cut(s) 125, 140, 226
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 6 cut(s) 137, 141, 155, 260, 272, 332
BspANI GGCC 3 cut(s) 125, 140, 226
BspCNI CTCAG 1 cut(s) 183
BspFNI CGCG 1 cut(s) 332
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 3 cut(s) 84, 126, 336
BsrDI GCAATG 1 cut(s) 282
BsrGI TGTACA 1 cut(s) 423
BssECI CCNNGG 3 cut(s) 179, 221, 243
BssMI GATC 1 cut(s) 493
BssSI CACGAG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 2 cut(s) 346, 354
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 2 cut(s) 21, 228
BstDEI CTNAG 2 cut(s) 170, 402
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 2 cut(s) 25, 332
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 3 cut(s) 283, 335, 435
BstMBI GATC 1 cut(s) 493
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 4 cut(s) 143, 152, 269, 329
BstNI CCWGG 1 cut(s) 180
BstNSI RCATGY 3 cut(s) 424, 430, 469
BstSCI CCNGG 5 cut(s) 78, 85, 98, 178, 336
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 3 cut(s) 130, 145, 523
BstV2I GAAGAC 1 cut(s) 209
BstZI CGGCCG 1 cut(s) 138
BsuRI GGCC 3 cut(s) 125, 140, 226
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 21, 228
CciI TCATGA 1 cut(s) 496
CciNI GCGGCCGC 1 cut(s) 138
CfoI GCGC 2 cut(s) 25, 332
Cfr13I GGNCC 3 cut(s) 124, 176, 334
Csp6I GTAC 5 cut(s) 11, 31, 41, 418, 424
CviAII CATG 4 cut(s) 421, 427, 466, 497
CviQI GTAC 5 cut(s) 11, 31, 41, 418, 424
DdeI CTNAG 2 cut(s) 170, 402
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
EclXI CGGCCG 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 176, 334
Eco52I CGGCCG 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 4 cut(s) 424, 430, 469, 500
FaiI YATR 7 cut(s) 106, 422, 428, 467, 479, 489, 498
FaqI GGGAC 3 cut(s) 225, 320, 363
FatI CATG 4 cut(s) 420, 426, 465, 496
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 512
FokI GGATG 1 cut(s) 424
Fsp4HI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 512
FspBI CTAG 2 cut(s) 110, 535
GlaI GCGC 2 cut(s) 24, 331
GluI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 512
HaeIII GGCC 3 cut(s) 125, 140, 226
HapII CCGG 4 cut(s) 79, 86, 100, 338
HhaI GCGC 2 cut(s) 25, 332
Hin1II CATG 4 cut(s) 424, 430, 469, 500
Hin6I GCGC 2 cut(s) 23, 330
HinP1I GCGC 2 cut(s) 23, 330
HinfI GANTC 5 cut(s) 238, 361, 383, 395, 530
HpaII CCGG 4 cut(s) 79, 86, 100, 338
HphI GGTGA 1 cut(s) 378
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 4 cut(s) 73, 173, 222, 247
Hpy188III TCNNGA 4 cut(s) 200, 266, 437, 497
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 346, 354
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 4 cut(s) 166, 253, 415, 511
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 152, 269, 329
HpyF3I CTNAG 2 cut(s) 170, 402
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 4 cut(s) 424, 430, 469, 500
HspAI GCGC 2 cut(s) 23, 330
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 1 cut(s) 493
Lsp1109I GCAGC 3 cut(s) 130, 145, 523
LweI GCATC 3 cut(s) 142, 402, 523
MaeI CTAG 2 cut(s) 110, 535
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 384
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 1 cut(s) 209
MluCI AATT 1 cut(s) 366
MlyI GAGTC 5 cut(s) 247, 370, 389, 392, 524
MseI TTAA 2 cut(s) 192, 378
MslI CAYNNNNRTG 3 cut(s) 425, 438, 501
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 4 cut(s) 79, 86, 100, 338
MspR9I CCNGG 5 cut(s) 80, 87, 100, 180, 338
MvaI CCWGG 1 cut(s) 180
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 4 cut(s) 143, 152, 269, 329
NciI CCSGG 4 cut(s) 80, 87, 100, 338
NdeII GATC 1 cut(s) 493
NlaIII CATG 4 cut(s) 424, 430, 469, 500
NlaIV GGNNCC 3 cut(s) 84, 126, 336
NmeAIII GCCGAG 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 384
NotI GCGGCCGC 1 cut(s) 138
NspI RCATGY 3 cut(s) 424, 430, 469
PagI TCATGA 1 cut(s) 496
PciI ACATGT 2 cut(s) 420, 426
Pfl23II CGTACG 1 cut(s) 40
PfoI TCCNGGA 2 cut(s) 78, 336
PkrI GCNGC 8 cut(s) 139, 142, 145, 157, 160, 262, 274, 513
PleI GAGTC 5 cut(s) 246, 369, 389, 391, 524
PpsI GAGTC 5 cut(s) 246, 369, 389, 391, 524
PscI ACATGT 2 cut(s) 420, 426
PshBI ATTAAT 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspLI CGTACG 1 cut(s) 40
PspN4I GGNNCC 3 cut(s) 84, 126, 336
PspPI GGNCC 3 cut(s) 124, 176, 334
RsaI GTAC 5 cut(s) 12, 32, 42, 419, 425
RsaNI GTAC 5 cut(s) 11, 31, 41, 418, 424
RseI CAYNNNNRTG 3 cut(s) 425, 438, 501
SaqAI TTAA 2 cut(s) 192, 378
SatI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 512
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 3 cut(s) 124, 176, 334
SchI GAGTC 5 cut(s) 247, 370, 389, 392, 524
ScrFI CCNGG 5 cut(s) 80, 87, 100, 180, 338
SetI ASST 5 cut(s) 119, 171, 252, 284, 321
SfaNI GCATC 3 cut(s) 142, 402, 523
SinI GGWCC 2 cut(s) 176, 334
SmiMI CAYNNNNRTG 3 cut(s) 425, 438, 501
SmlI CTYRAG 1 cut(s) 264
SmoI CTYRAG 1 cut(s) 264
Sse9I AATT 1 cut(s) 366
SsiI CCGC 6 cut(s) 137, 141, 155, 260, 272, 332
SspMI CTAG 2 cut(s) 110, 535
StyD4I CCNGG 5 cut(s) 78, 85, 98, 178, 336
TaaI ACNGT 2 cut(s) 346, 354
TaiI ACGT 1 cut(s) 252
TaqI TCGA 2 cut(s) 236, 311
TasI AATT 1 cut(s) 366
TatI WGTACW 4 cut(s) 10, 30, 417, 423
TauI GCSGC 5 cut(s) 140, 143, 158, 263, 275
Tru1I TTAA 2 cut(s) 192, 378
Tru9I TTAA 2 cut(s) 192, 378
TseFI GTSAC 1 cut(s) 384
TseI GCWGC 3 cut(s) 143, 158, 511
Tsp45I GTSAC 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 329
VpaK11BI GGWCC 2 cut(s) 176, 334
VspI ATTAAT 1 cut(s) 378
XapI RAATTY 1 cut(s) 366
XceI RCATGY 3 cut(s) 424, 430, 469
XspI CTAG 2 cut(s) 110, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.