Rroxscaffold_3G00228980
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005624
Physical Location & Seq
Reverse (-)
13491184 .. 13491792
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228980.1

Sequence Viewer

Length: 609 bp
ATGACCCAAGAAATTAATCAAACCCTCCGGGCAGATATGGAGGAAGCACACGAGCCTGCAGTCAAGTACAGAGGCGTACGCAAGCGAAAATGGGGTAAATGGGTGTCCGAAATCCGGCAACCGGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCCGAAATGGCCGCCGCCGCCTACGATGCGGCGGCATTGCACCTTAGAGGACCAGGGGTCCTGTTTAACTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCTTCGGACGTGCAGTTGGCGGCTCAAGAGGCGGCAATGAGGTTTAGGAGACTGCCAATGGGGTCGGTTTCGACCGAGGTGGCTGGTGGCTCGAGTGGTGGCGCGGCGCCGGTGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCGCCATTAGACTCGCCTAAGATGTGGATGCAGCACATGTACATGTCTCATCGTGATGAGGCTTTGTTTTTGGGGAGGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGACGGTTTGCAGCATCAGTCCATTTGGGACTCCTAGTTTATTACTTTTCATATATAAATGA

Protein Analysis

202

Amino Acids

22.4

Weight (kDa)

9.2

Isoelectric Point (pI)

61.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 22 - 71 2.4e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccII CGCG 1 cut(s) 380
AciI CCGC 8 cut(s) 174, 177, 180, 191, 194, 296, 308, 380
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 414
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 3 cut(s) 68, 78, 473
AfiI CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
AflIII ACRYGT 2 cut(s) 468, 474
AhdI GACNNNNNGTC 1 cut(s) 218
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 319, 483
Ama87I CYCGRG 1 cut(s) 367
AoxI GGCC 3 cut(s) 159, 171, 260
ApeKI GCWGC 2 cut(s) 463, 558
ApoI RAATTY 1 cut(s) 414
AseI ATTAAT 2 cut(s) 15, 426
AspLEI GCGC 2 cut(s) 380, 385
AspS9I GGNCC 3 cut(s) 160, 212, 220
AsuC2I CCSGG 3 cut(s) 29, 123, 136
AsuHPI GGTGA 1 cut(s) 400
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 2 cut(s) 212, 220
BanI GGYRCC 1 cut(s) 382
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 475, 570
BccI CCATC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 216
BclI TGATCA 1 cut(s) 540
BcnI CCSGG 3 cut(s) 29, 123, 136
BcoDI GTCTC 2 cut(s) 319, 483
BfaI CTAG 2 cut(s) 146, 582
BfmI CTRYAG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 386
Bme1390I CCNGG 4 cut(s) 29, 123, 136, 216
Bme18I GGWCC 2 cut(s) 212, 220
BmeRI GACNNNNNGTC 1 cut(s) 218
BmeT110I CYCGRG 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 160, 212, 220
BmiI GGNNCC 3 cut(s) 162, 221, 384
BmrFI CCNGG 4 cut(s) 29, 123, 136, 216
BmsI GCATC 3 cut(s) 178, 450, 570
BpiI GAAGAC 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 3 cut(s) 29, 123, 136
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 215, 257, 351
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
Bse118I RCCGGY 1 cut(s) 385
Bse3DI GCAATG 2 cut(s) 197, 318
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 3 cut(s) 215, 257, 351
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
BseMI GCAATG 2 cut(s) 197, 318
BseXI GCAGC 2 cut(s) 475, 570
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 1 cut(s) 380
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 3 cut(s) 161, 173, 262
BshNI GGYRCC 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 5 cut(s) 28, 115, 122, 136, 386
BsiWI CGTACG 1 cut(s) 76
BslFI GGGAC 2 cut(s) 411, 589
BslI CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
BsmAI GTCTC 2 cut(s) 319, 483
BsmFI GGGAC 2 cut(s) 411, 589
BsnI GGCC 3 cut(s) 161, 173, 262
BsoBI CYCGRG 1 cut(s) 367
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 1 cut(s) 540
BspACI CCGC 8 cut(s) 174, 177, 180, 191, 194, 296, 308, 380
BspANI GGCC 3 cut(s) 161, 173, 262
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 543
BspLI GGNNCC 3 cut(s) 162, 221, 384
BspMAI CTGCAG 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 2 cut(s) 197, 318
BsrFI RCCGGY 1 cut(s) 385
BsrGI TGTACA 1 cut(s) 471
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 215, 257, 351
BssMI GATC 1 cut(s) 540
BssNI GRCGYC 1 cut(s) 383
BssSI CACGAG 1 cut(s) 50
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 394, 402, 553
BstACI GRCGYC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 57, 83, 264
BstDEI CTNAG 2 cut(s) 206, 450
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 1 cut(s) 380
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 2 cut(s) 380, 385
BstKTI GATC 1 cut(s) 543
BstMAI GTCTC 2 cut(s) 319, 483
BstMBI GATC 1 cut(s) 540
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 3 cut(s) 179, 188, 305
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 3 cut(s) 472, 478, 516
BstSCI CCNGG 4 cut(s) 27, 121, 134, 214
BstSFI CTRYAG 1 cut(s) 57
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 2 cut(s) 475, 570
BstV2I GAAGAC 1 cut(s) 245
BsuRI GGCC 3 cut(s) 161, 173, 262
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 1 cut(s) 465
Cac8I GCNNGC 3 cut(s) 57, 83, 264
CciI TCATGA 1 cut(s) 543
CfoI GCGC 2 cut(s) 380, 385
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 160, 212, 220
Csp6I GTAC 3 cut(s) 67, 77, 472
CviAII CATG 4 cut(s) 469, 475, 513, 544
CviQI GTAC 3 cut(s) 67, 77, 472
DdeI CTNAG 2 cut(s) 206, 450
DinI GGCGCC 1 cut(s) 384
DpnI GATC 1 cut(s) 542
DpnII GATC 1 cut(s) 540
DriI GACNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 1 cut(s) 171
Eam1105I GACNNNNNGTC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 212, 220
Eco88I CYCGRG 1 cut(s) 367
EcoO109I RGGNCCY 2 cut(s) 160, 220
EcoRII CCWGG 1 cut(s) 214
EgeI GGCGCC 1 cut(s) 384
EheI GGCGCC 1 cut(s) 384
FaeI CATG 4 cut(s) 472, 478, 516, 547
FaqI GGGAC 2 cut(s) 411, 589
FatI CATG 4 cut(s) 468, 474, 512, 543
FbaI TGATCA 1 cut(s) 540
FokI GGATG 1 cut(s) 472
FspBI CTAG 2 cut(s) 146, 582
GlaI GCGC 2 cut(s) 379, 384
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 3 cut(s) 161, 173, 262
HapII CCGG 5 cut(s) 28, 115, 122, 136, 386
HhaI GCGC 2 cut(s) 380, 385
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 4 cut(s) 472, 478, 516, 547
Hin6I GCGC 2 cut(s) 378, 383
HinP1I GCGC 2 cut(s) 378, 383
HinfI GANTC 5 cut(s) 274, 409, 431, 443, 577
HpaII CCGG 5 cut(s) 28, 115, 122, 136, 386
HphI GGTGA 1 cut(s) 400
Hpy188I TCNGA 3 cut(s) 109, 258, 283
Hpy188III TCNNGA 4 cut(s) 236, 302, 485, 544
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 3 cut(s) 394, 402, 553
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 5 cut(s) 59, 202, 289, 463, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 188, 305
HpyF3I CTNAG 2 cut(s) 206, 450
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 4 cut(s) 472, 478, 516, 547
HspAI GCGC 2 cut(s) 378, 383
KasI GGCGCC 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 540
Kzo9I GATC 1 cut(s) 540
Lsp1109I GCAGC 2 cut(s) 475, 570
LweI GCATC 3 cut(s) 178, 450, 570
MaeI CTAG 2 cut(s) 146, 582
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 388
MalI GATC 1 cut(s) 542
MboI GATC 1 cut(s) 540
MboII GAAGA 1 cut(s) 245
MluCI AATT 2 cut(s) 12, 414
Mly113I GGCGCC 1 cut(s) 383
MlyI GAGTC 5 cut(s) 283, 418, 437, 440, 571
MseI TTAA 3 cut(s) 15, 228, 426
MslI CAYNNNNRTG 2 cut(s) 473, 486
MspI CCGG 5 cut(s) 28, 115, 122, 136, 386
MspR9I CCNGG 4 cut(s) 29, 123, 136, 216
MteI GCGCNGCGC 1 cut(s) 381
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 3 cut(s) 179, 188, 305
NarI GGCGCC 1 cut(s) 383
NciI CCSGG 3 cut(s) 29, 123, 136
NdeII GATC 1 cut(s) 540
NlaIII CATG 4 cut(s) 472, 478, 516, 547
NlaIV GGNNCC 3 cut(s) 162, 221, 384
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 3 cut(s) 472, 478, 516
PaeR7I CTCGAG 1 cut(s) 367
PagI TCATGA 1 cut(s) 543
PciI ACATGT 2 cut(s) 468, 474
PcsI WCGNNNNNNNCGW 1 cut(s) 162
Pfl23II CGTACG 1 cut(s) 76
PleI GAGTC 5 cut(s) 282, 417, 437, 439, 571
PluTI GGCGCC 1 cut(s) 386
PpsI GAGTC 5 cut(s) 282, 417, 437, 439, 571
PpuMI RGGWCCY 1 cut(s) 220
PscI ACATGT 2 cut(s) 468, 474
PshBI ATTAAT 2 cut(s) 15, 426
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspLI CGTACG 1 cut(s) 76
PspN4I GGNNCC 3 cut(s) 162, 221, 384
PspPI GGNCC 3 cut(s) 160, 212, 220
PspPPI RGGWCCY 1 cut(s) 220
PspXI VCTCGAGB 1 cut(s) 367
PstI CTGCAG 1 cut(s) 61
RsaI GTAC 3 cut(s) 68, 78, 473
RsaNI GTAC 3 cut(s) 67, 77, 472
RseI CAYNNNNRTG 2 cut(s) 473, 486
SaqAI TTAA 3 cut(s) 15, 228, 426
Sau3AI GATC 1 cut(s) 540
Sau96I GGNCC 3 cut(s) 160, 212, 220
SchI GAGTC 5 cut(s) 283, 418, 437, 440, 571
ScrFI CCNGG 4 cut(s) 29, 123, 136, 216
SetI ASST 5 cut(s) 155, 207, 288, 320, 357
SfaNI GCATC 3 cut(s) 178, 450, 570
SfcI CTRYAG 1 cut(s) 57
SfoI GGCGCC 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 367
SgrAI CRCCGGYG 1 cut(s) 385
SinI GGWCC 2 cut(s) 212, 220
SlaI CTCGAG 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 473, 486
SmlI CTYRAG 2 cut(s) 300, 367
SmoI CTYRAG 2 cut(s) 300, 367
Sse9I AATT 2 cut(s) 12, 414
SsiI CCGC 8 cut(s) 174, 177, 180, 191, 194, 296, 308, 380
SspDI GGCGCC 1 cut(s) 382
SspMI CTAG 2 cut(s) 146, 582
StyD4I CCNGG 4 cut(s) 27, 121, 134, 214
TaaI ACNGT 3 cut(s) 394, 402, 553
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 272, 347, 368
TaqII GACCGA 1 cut(s) 365
TasI AATT 2 cut(s) 12, 414
TatI WGTACW 2 cut(s) 66, 471
TauI GCSGC 8 cut(s) 176, 179, 182, 194, 197, 299, 311, 383
Tru1I TTAA 3 cut(s) 15, 228, 426
Tru9I TTAA 3 cut(s) 15, 228, 426
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 2 cut(s) 463, 558
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 1 cut(s) 586
VpaK11BI GGWCC 2 cut(s) 212, 220
VspI ATTAAT 2 cut(s) 15, 426
XapI RAATTY 1 cut(s) 414
XceI RCATGY 3 cut(s) 472, 478, 516
XhoI CTCGAG 1 cut(s) 367
XspI CTAG 2 cut(s) 146, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.