MD15G1396500.v1.1
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005624
Physical Location & Seq
Forward (+)
49614193 .. 49614798
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1396500.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGAACCCCAGGCTGCAGACTGCCGCGTCTCTCCGGCAAGATACAAAGGAGTTCGCAAGCGAAAATGGGGCAAATGGGTGTCCGAAATCCGCGAACCAGGAAAGAAAACCAGAATATGGCTAGGAAGCTACGAGGCGCCAGAAATGGCAGCTGCGGCCTATGATGCAGCGGCATTGCACCTCAAAGGGTGTGGGGCGGCGCTTAATTTTCCTGAAATGGCTGATAGTCTTCCGAGGCCGGCCAGTTCAAGCGCAATGGACGTGCAACTAGCGGCTCAAGAGGCTGCATTGAGGGTTAGACGACAGCAGCTGCCAATGGAGGCATTTCCAAAGGAGGAGGCGGGTGACTCGTCGAGGGGTTTGAGCTCAGCGCCAGTGACGGTGGGACTGTCTCCGAGTCAAATCCAGGCGATTAATGAGTCGCCGTTGGACTCGCCAAAGATGTGGATGCAGTACATGTGCAGGGCTCATGGCCATGAGGCTCCGTCGTCGTTAGGGGGAGACCTGTGGGACACTGATAATTCCAGTCCAAACAAGTACTATGAAGGTGATGTCGAGTCAATGGACTACGAAGATATGCAGCATTGGTCCATTTGGGATCCCTAG

Protein Analysis

202

Amino Acids

22.23

Weight (kDa)

5.28

Isoelectric Point (pI)

63.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 14 - 63 2.1e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 117
AccII CGCG 2 cut(s) 27, 93
AciI CCGC 7 cut(s) 25, 91, 155, 170, 197, 272, 341
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 2 cut(s) 240, 472
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 2 cut(s) 455, 539
AfiI CCNNNNNNNGG 1 cut(s) 117
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 1 cut(s) 249
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 3 cut(s) 9, 97, 405
AluBI AGCT 4 cut(s) 129, 152, 310, 366
AluI AGCT 4 cut(s) 129, 152, 310, 366
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 3 cut(s) 34, 396, 495
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 4 cut(s) 156, 236, 240, 472
ApeKI GCWGC 8 cut(s) 14, 149, 152, 167, 284, 307, 310, 580
AseI ATTAAT 1 cut(s) 414
AspLEI GCGC 4 cut(s) 139, 202, 254, 373
AspS9I GGNCC 1 cut(s) 588
AsuHPI GGTGA 2 cut(s) 356, 560
AvaII GGWCC 1 cut(s) 588
BalI TGGCCA 1 cut(s) 474
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 136
BanII GRGCYC 2 cut(s) 368, 469
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 3 cut(s) 11, 99, 407
BcoDI GTCTC 3 cut(s) 34, 396, 495
BfaI CTAG 3 cut(s) 122, 269, 604
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 3 cut(s) 140, 203, 374
BlpI GCTNAGC 1 cut(s) 367
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 3 cut(s) 11, 99, 407
Bme18I GGWCC 1 cut(s) 588
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 588
BmiI GGNNCC 4 cut(s) 6, 138, 483, 600
BmrFI CCNGG 3 cut(s) 11, 99, 407
BmsI GCATC 2 cut(s) 154, 438
BpiI GAAGAC 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 9, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 3 cut(s) 243, 374, 525
Bse3DI GCAATG 2 cut(s) 173, 261
BseBI CCWGG 3 cut(s) 11, 99, 407
BseDI CCNNGG 2 cut(s) 9, 233
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMI GCAATG 2 cut(s) 173, 261
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 3 cut(s) 243, 374, 525
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
BsgI GTGCAG 1 cut(s) 481
Bsh1236I CGCG 2 cut(s) 27, 93
BshFI GGCC 4 cut(s) 158, 238, 242, 474
BshNI GGYRCC 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 2 cut(s) 35, 239
BslFI GGGAC 2 cut(s) 399, 524
BslI CCNNNNNNNGG 1 cut(s) 117
BsmAI GTCTC 3 cut(s) 34, 396, 495
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 2 cut(s) 399, 524
BsnI GGCC 4 cut(s) 158, 238, 242, 474
Bso31I GGTCTC 1 cut(s) 495
Bsp1286I GDGCHC 2 cut(s) 368, 469
Bsp143I GATC 1 cut(s) 598
Bsp1720I GCTNAGC 1 cut(s) 367
BspACI CCGC 7 cut(s) 25, 91, 155, 170, 197, 272, 341
BspANI GGCC 4 cut(s) 158, 238, 242, 474
BspCNI CTCAG 1 cut(s) 380
BspFNI CGCG 2 cut(s) 27, 93
BspLI GGNNCC 4 cut(s) 6, 138, 483, 600
BspMAI CTGCAG 1 cut(s) 19
BspPI GGATC 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 136
BspTNI GGTCTC 1 cut(s) 495
BsrDI GCAATG 2 cut(s) 173, 261
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 3 cut(s) 243, 374, 525
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 2 cut(s) 9, 233
BssMI GATC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 137
Bst2UI CCWGG 3 cut(s) 11, 99, 407
Bst4CI ACNGT 2 cut(s) 382, 390
BstACI GRCGYC 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 59, 240
BstDEI CTNAG 1 cut(s) 367
BstF5I GGATG 1 cut(s) 453
BstFNI CGCG 2 cut(s) 27, 93
BstH2I RGCGCY 3 cut(s) 140, 203, 374
BstHHI GCGC 4 cut(s) 139, 202, 254, 373
BstKTI GATC 1 cut(s) 601
BstMAI GTCTC 3 cut(s) 34, 396, 495
BstMBI GATC 1 cut(s) 598
BstMWI GCNNNNNNNGC 3 cut(s) 155, 164, 281
BstNI CCWGG 3 cut(s) 11, 99, 407
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 3 cut(s) 9, 97, 405
BstSFI CTRYAG 1 cut(s) 15
BstUI CGCG 2 cut(s) 27, 93
BstV1I GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 598
BstXI CCANNNNNNTGG 1 cut(s) 444
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 4 cut(s) 158, 238, 242, 474
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 381, 513
Cac8I GCNNGC 2 cut(s) 59, 240
CaiI CAGNNNCTG 1 cut(s) 310
CfoI GCGC 4 cut(s) 139, 202, 254, 373
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 588
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 454, 538
CspCI CAANNNNNGTGG 2 cut(s) 172, 207
CviAII CATG 3 cut(s) 457, 470, 476
CviQI GTAC 2 cut(s) 454, 538
DdeI CTNAG 1 cut(s) 367
DinI GGCGCC 1 cut(s) 138
DpnI GATC 1 cut(s) 600
DpnII GATC 1 cut(s) 598
DrdI GACNNNNNNGTC 1 cut(s) 26
DseDI GACNNNNNNGTC 1 cut(s) 26
EaeI YGGCCR 2 cut(s) 240, 472
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 2 cut(s) 368, 469
Eco31I GGTCTC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 588
Eco53kI GAGCTC 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 366
EcoRII CCWGG 3 cut(s) 9, 97, 405
EcoT38I GRGCYC 2 cut(s) 368, 469
EgeI GGCGCC 1 cut(s) 138
EheI GGCGCC 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 3 cut(s) 460, 473, 479
FaiI YATR 7 cut(s) 118, 162, 458, 471, 477, 543, 578
FaqI GGGAC 2 cut(s) 399, 524
FatI CATG 3 cut(s) 456, 469, 475
FauI CCCGC 1 cut(s) 334
FokI GGATG 1 cut(s) 460
FriOI GRGCYC 2 cut(s) 368, 469
FseI GGCCGGCC 1 cut(s) 242
FspBI CTAG 3 cut(s) 122, 269, 604
GlaI GCGC 4 cut(s) 138, 201, 253, 372
HaeII RGCGCY 3 cut(s) 140, 203, 374
HaeIII GGCC 4 cut(s) 158, 238, 242, 474
HapII CCGG 2 cut(s) 35, 239
HgaI GACGC 1 cut(s) 16
HhaI GCGC 4 cut(s) 139, 202, 254, 373
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 3 cut(s) 460, 473, 479
Hin6I GCGC 4 cut(s) 137, 200, 252, 371
HinP1I GCGC 4 cut(s) 137, 200, 252, 371
HinfI GANTC 5 cut(s) 347, 397, 419, 431, 557
HpaII CCGG 2 cut(s) 35, 239
HphI GGTGA 2 cut(s) 356, 560
Hpy188I TCNGA 3 cut(s) 85, 234, 396
Hpy188III TCNNGA 2 cut(s) 212, 278
Hpy99I CGWCG 3 cut(s) 355, 490, 493
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 2 cut(s) 382, 390
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 8 cut(s) 17, 167, 178, 265, 287, 451, 462, 580
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 164, 281
HpyF3I CTNAG 1 cut(s) 367
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 3 cut(s) 460, 473, 479
HspAI GCGC 4 cut(s) 137, 200, 252, 371
KasI GGCGCC 1 cut(s) 136
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Kzo9I GATC 1 cut(s) 598
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
LweI GCATC 2 cut(s) 154, 438
MaeI CTAG 3 cut(s) 122, 269, 604
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 344, 376
MalI GATC 1 cut(s) 600
MboI GATC 1 cut(s) 598
MboII GAAGA 2 cut(s) 221, 584
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 2 cut(s) 368, 469
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 2 cut(s) 205, 520
MluNI TGGCCA 1 cut(s) 474
Mly113I GGCGCC 1 cut(s) 137
MlyI GAGTC 5 cut(s) 341, 406, 425, 428, 566
MmeI TCCRAC 1 cut(s) 408
Mox20I TGGCCA 1 cut(s) 474
MroNI GCCGGC 1 cut(s) 238
MscI TGGCCA 1 cut(s) 474
MseI TTAA 2 cut(s) 204, 414
MslI CAYNNNNRTG 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 474
MspA1I CMGCKG 3 cut(s) 152, 170, 310
MspI CCGG 2 cut(s) 35, 239
MspR9I CCNGG 3 cut(s) 11, 99, 407
MvaI CCWGG 3 cut(s) 11, 99, 407
MvnI CGCG 2 cut(s) 27, 93
MwoI GCNNNNNNNGC 3 cut(s) 155, 164, 281
NaeI GCCGGC 1 cut(s) 240
NarI GGCGCC 1 cut(s) 137
NdeII GATC 1 cut(s) 598
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 3 cut(s) 460, 473, 479
NlaIV GGNNCC 4 cut(s) 6, 138, 483, 600
NmuCI GTSAC 2 cut(s) 344, 376
NspI RCATGY 1 cut(s) 460
PciI ACATGT 1 cut(s) 456
PdiI GCCGGC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 117
PleI GAGTC 5 cut(s) 341, 405, 425, 427, 565
PluTI GGCGCC 1 cut(s) 140
PpsI GAGTC 5 cut(s) 341, 405, 425, 427, 565
PscI ACATGT 1 cut(s) 456
PshBI ATTAAT 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 3 cut(s) 9, 97, 405
PspGI CCWGG 3 cut(s) 9, 97, 405
PspN4I GGNNCC 4 cut(s) 6, 138, 483, 600
PspPI GGNCC 1 cut(s) 588
PstI CTGCAG 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 598
PvuII CAGCTG 2 cut(s) 152, 310
RigI GGCCGGCC 1 cut(s) 242
RsaI GTAC 2 cut(s) 455, 539
RsaNI GTAC 2 cut(s) 454, 538
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 2 cut(s) 204, 414
Sau3AI GATC 1 cut(s) 598
Sau96I GGNCC 1 cut(s) 588
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 5 cut(s) 341, 406, 425, 428, 566
ScrFI CCNGG 3 cut(s) 11, 99, 407
SduI GDGCHC 2 cut(s) 368, 469
SetI ASST 8 cut(s) 131, 154, 183, 264, 312, 368, 507, 550
SfaNI GCATC 2 cut(s) 154, 438
SfcI CTRYAG 1 cut(s) 15
SfoI GGCGCC 1 cut(s) 138
SinI GGWCC 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
Sse9I AATT 2 cut(s) 205, 520
SsiI CCGC 7 cut(s) 25, 91, 155, 170, 197, 272, 341
SspDI GGCGCC 1 cut(s) 136
SspMI CTAG 3 cut(s) 122, 269, 604
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 3 cut(s) 9, 97, 405
TaaI ACNGT 2 cut(s) 382, 390
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 353, 555
TasI AATT 2 cut(s) 205, 520
TatI WGTACW 2 cut(s) 453, 537
TauI GCSGC 5 cut(s) 27, 158, 173, 200, 275
Tru1I TTAA 2 cut(s) 204, 414
Tru9I TTAA 2 cut(s) 204, 414
TscAI CASTG 2 cut(s) 381, 520
TseFI GTSAC 2 cut(s) 344, 376
TseI GCWGC 8 cut(s) 14, 149, 152, 167, 284, 307, 310, 580
Tsp45I GTSAC 2 cut(s) 344, 376
TspDTI ATGAA 1 cut(s) 558
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 2 cut(s) 381, 520
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 588
VspI ATTAAT 1 cut(s) 414
XceI RCATGY 1 cut(s) 460
XspI CTAG 3 cut(s) 122, 269, 604
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.