Rroxscaffold_3G00228920
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005624
Physical Location & Seq
Reverse (-)
13389605 .. 13390153
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228920.1

Sequence Viewer

Length: 549 bp
ATGAAGGAAGTACACGAGCCTGCAGCCAAGTACAGAGGCGTACGCAAGCGAAAATGGGGCAAATGGGTGTCTGAAATCCGCGAGCCAGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCCGAAATGGCGGCCGCGGCCTACGATGCGGTGGCTTTGCACCTCAGAGGACCAGGGGTGTTGCTTAACTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCCTCGGACGTGCAGTTGGCGGCTCAAGAGGCGGCAATGAGGTTTAGGAGACTGCCAATGGGGTCGGTTTCGACGGAGGTGGCTGGTGGCTCGAGTGGTGGCGCGGTGCCGGTGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCGCCATTGGACTCGCCTAAGATGTGGATGCAGTACATGTACATGTCTCATCGTGATGAGGCTTTTTTTGTTCAGGAGGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGATGGTTTGCAGCATCAGTCCATTTGGGACTCCTAG

Protein Analysis

182

Amino Acids

20.19

Weight (kDa)

5.73

Isoelectric Point (pI)

74.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 10 - 59 1.2e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccII CGCG 3 cut(s) 81, 143, 344
AciI CCGC 8 cut(s) 79, 137, 141, 143, 155, 260, 272, 344
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 1 cut(s) 378
AfaI GTAC 5 cut(s) 12, 32, 42, 431, 437
AfiI CCNNNNNNNGG 2 cut(s) 105, 133
AflIII ACRYGT 2 cut(s) 432, 438
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 2 cut(s) 85, 178
AluBI AGCT 1 cut(s) 117
AluI AGCT 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 283, 447
Ama87I CYCGRG 1 cut(s) 331
AoxI GGCC 4 cut(s) 123, 138, 144, 224
ApeKI GCWGC 2 cut(s) 23, 523
ApoI RAATTY 1 cut(s) 378
AseI ATTAAT 1 cut(s) 390
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 2 cut(s) 124, 176
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 346
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 2 cut(s) 35, 535
BccI CCATC 2 cut(s) 490, 509
BciT130I CCWGG 2 cut(s) 87, 180
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 283, 447
BfaI CTAG 2 cut(s) 110, 547
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 7 cut(s) 24, 138, 141, 144, 261, 273, 524
BlsI GCNGC 7 cut(s) 25, 139, 142, 145, 262, 274, 525
Bme1390I CCNGG 3 cut(s) 87, 100, 180
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 124, 176
BmiI GGNNCC 2 cut(s) 126, 348
BmrFI CCNGG 3 cut(s) 87, 100, 180
BmsI GCATC 3 cut(s) 142, 414, 535
BpiI GAAGAC 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 100
BsaJI CCNNGG 4 cut(s) 141, 179, 221, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 133
Bse118I RCCGGY 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 2 cut(s) 87, 180
BseDI CCNNGG 4 cut(s) 141, 179, 221, 243
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 2 cut(s) 105, 133
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 184
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 2 cut(s) 35, 535
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 3 cut(s) 81, 143, 344
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 4 cut(s) 125, 140, 146, 226
BshNI GGYRCC 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 2 cut(s) 100, 350
BsiWI CGTACG 1 cut(s) 40
BslFI GGGAC 2 cut(s) 225, 375
BslI CCNNNNNNNGG 2 cut(s) 105, 133
BsmAI GTCTC 2 cut(s) 283, 447
BsmFI GGGAC 2 cut(s) 225, 375
BsnI GGCC 4 cut(s) 125, 140, 146, 226
BsoBI CYCGRG 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 1 cut(s) 505
BspACI CCGC 8 cut(s) 79, 137, 141, 143, 155, 260, 272, 344
BspANI GGCC 4 cut(s) 125, 140, 146, 226
BspCNI CTCAG 1 cut(s) 183
BspFNI CGCG 3 cut(s) 81, 143, 344
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 126, 348
BspMAI CTGCAG 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 349
BsrGI TGTACA 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 4 cut(s) 141, 179, 221, 243
BssMI GATC 1 cut(s) 505
BssSI CACGAG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 2 cut(s) 87, 180
Bst4CI ACNGT 2 cut(s) 358, 366
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 4 cut(s) 21, 47, 83, 228
BstDEI CTNAG 2 cut(s) 170, 414
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 3 cut(s) 81, 143, 344
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 1 cut(s) 508
BstMAI GTCTC 2 cut(s) 283, 447
BstMBI GATC 1 cut(s) 505
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 269
BstNI CCWGG 2 cut(s) 87, 180
BstNSI RCATGY 3 cut(s) 436, 442, 481
BstSCI CCNGG 3 cut(s) 85, 98, 178
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 3 cut(s) 81, 143, 344
BstV1I GCAGC 2 cut(s) 35, 535
BstV2I GAAGAC 1 cut(s) 209
BstZI CGGCCG 1 cut(s) 138
BsuRI GGCC 4 cut(s) 125, 140, 146, 226
BtgI CCRYGG 1 cut(s) 141
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 429
Cac8I GCNNGC 4 cut(s) 21, 47, 83, 228
CciI TCATGA 1 cut(s) 508
CciNI GCGGCCGC 1 cut(s) 138
CfoI GCGC 1 cut(s) 344
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 124, 176
Cfr42I CCGCGG 1 cut(s) 144
Csp6I GTAC 5 cut(s) 11, 31, 41, 430, 436
CviAII CATG 4 cut(s) 433, 439, 478, 509
CviQI GTAC 5 cut(s) 11, 31, 41, 430, 436
DdeI CTNAG 2 cut(s) 170, 414
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
EclXI CGGCCG 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 176
Eco52I CGGCCG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 85, 178
FaeI CATG 4 cut(s) 436, 442, 481, 512
FaiI YATR 7 cut(s) 106, 434, 440, 479, 491, 501, 510
FaqI GGGAC 2 cut(s) 225, 375
FatI CATG 4 cut(s) 432, 438, 477, 508
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 7 cut(s) 24, 138, 141, 144, 261, 273, 524
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 7 cut(s) 24, 138, 141, 144, 261, 273, 524
FspBI CTAG 2 cut(s) 110, 547
GlaI GCGC 1 cut(s) 343
GluI GCNGC 7 cut(s) 24, 138, 141, 144, 261, 273, 524
HaeIII GGCC 4 cut(s) 125, 140, 146, 226
HapII CCGG 2 cut(s) 100, 350
HhaI GCGC 1 cut(s) 344
Hin1II CATG 4 cut(s) 436, 442, 481, 512
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 5 cut(s) 238, 373, 395, 407, 542
HpaII CCGG 2 cut(s) 100, 350
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 4 cut(s) 73, 173, 222, 247
Hpy188III TCNNGA 5 cut(s) 200, 266, 449, 470, 509
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 358, 366
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 5 cut(s) 23, 166, 253, 427, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 269
HpyF3I CTNAG 2 cut(s) 170, 414
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 4 cut(s) 436, 442, 481, 512
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 505
KspI CCGCGG 1 cut(s) 144
Kzo9I GATC 1 cut(s) 505
Lsp1109I GCAGC 2 cut(s) 35, 535
LweI GCATC 3 cut(s) 142, 414, 535
MaeI CTAG 2 cut(s) 110, 547
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 352
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MboII GAAGA 1 cut(s) 209
MluCI AATT 1 cut(s) 378
MlyI GAGTC 5 cut(s) 247, 382, 401, 404, 536
MseI TTAA 2 cut(s) 192, 390
MslI CAYNNNNRTG 3 cut(s) 437, 450, 513
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 2 cut(s) 100, 350
MspR9I CCNGG 3 cut(s) 87, 100, 180
MvaI CCWGG 2 cut(s) 87, 180
MvnI CGCG 3 cut(s) 81, 143, 344
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 269
NciI CCSGG 1 cut(s) 100
NdeII GATC 1 cut(s) 505
NlaIII CATG 4 cut(s) 436, 442, 481, 512
NlaIV GGNNCC 2 cut(s) 126, 348
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 352
NotI GCGGCCGC 1 cut(s) 138
NspI RCATGY 3 cut(s) 436, 442, 481
PaeR7I CTCGAG 1 cut(s) 331
PagI TCATGA 1 cut(s) 508
PciI ACATGT 2 cut(s) 432, 438
PcsI WCGNNNNNNNCGW 1 cut(s) 126
Pfl23II CGTACG 1 cut(s) 40
PkrI GCNGC 7 cut(s) 25, 139, 142, 145, 262, 274, 525
PleI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PpsI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PscI ACATGT 2 cut(s) 432, 438
PshBI ATTAAT 1 cut(s) 390
Psp6I CCWGG 2 cut(s) 85, 178
PspGI CCWGG 2 cut(s) 85, 178
PspLI CGTACG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 126, 348
PspPI GGNCC 2 cut(s) 124, 176
PspXI VCTCGAGB 1 cut(s) 331
PstI CTGCAG 1 cut(s) 25
RsaI GTAC 5 cut(s) 12, 32, 42, 431, 437
RsaNI GTAC 5 cut(s) 11, 31, 41, 430, 436
RseI CAYNNNNRTG 3 cut(s) 437, 450, 513
SacII CCGCGG 1 cut(s) 144
SaqAI TTAA 2 cut(s) 192, 390
SatI GCNGC 7 cut(s) 24, 138, 141, 144, 261, 273, 524
Sau3AI GATC 1 cut(s) 505
Sau96I GGNCC 2 cut(s) 124, 176
SchI GAGTC 5 cut(s) 247, 382, 401, 404, 536
ScrFI CCNGG 3 cut(s) 87, 100, 180
SetI ASST 5 cut(s) 119, 171, 252, 284, 321
SfaNI GCATC 3 cut(s) 142, 414, 535
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 331
Sfr303I CCGCGG 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 144
SinI GGWCC 1 cut(s) 176
SlaI CTCGAG 1 cut(s) 331
SmiMI CAYNNNNRTG 3 cut(s) 437, 450, 513
SmlI CTYRAG 2 cut(s) 264, 331
SmoI CTYRAG 2 cut(s) 264, 331
Sse9I AATT 1 cut(s) 378
SsiI CCGC 8 cut(s) 79, 137, 141, 143, 155, 260, 272, 344
SspMI CTAG 2 cut(s) 110, 547
StyD4I CCNGG 3 cut(s) 85, 98, 178
TaaI ACNGT 2 cut(s) 358, 366
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 236, 311, 332
TasI AATT 1 cut(s) 378
TatI WGTACW 4 cut(s) 10, 30, 429, 435
TauI GCSGC 5 cut(s) 140, 143, 146, 263, 275
Tru1I TTAA 2 cut(s) 192, 390
Tru9I TTAA 2 cut(s) 192, 390
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 23, 523
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 176
VspI ATTAAT 1 cut(s) 390
XapI RAATTY 1 cut(s) 378
XceI RCATGY 3 cut(s) 436, 442, 481
XhoI CTCGAG 1 cut(s) 331
XspI CTAG 2 cut(s) 110, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.