pycom15g35660
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005624
Physical Location & Seq
Forward (+)
35698469 .. 35699074
606 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35660.1

Sequence Viewer

Length: 606 bp
ATGGAATCCCAGGCTGCAGACTGCCGCGTCTCTCCGGCAAGATACAGAGGAGTTCGCAAGCGAAAATGGGGCAAATGGGTGTCCGAAATCCGCGAACCAGGAAAGAAAACCAGAATATGGCTAGGAAGCTACGAGGCACCAGAAATGGCAGCTGCGGCCTATGATGCAGCGGCATTGCACCTCAAAGGGCGTGGGGCGGCGCTTAATTTTCCTGAAATGGCTGATAGTCTGCCGAGGCCGGCCAGTTCAAGCGCAATGGACGTGCAACTAGCGGCTCAAGAGGCTGCATTGAGGGTTAGACGACAGCAGCTGCCAATGGAGGCATTTCCAACGGAGGAGGCGGGTGACTCGTCGAGGGGTTTGAGCTCAGCGCCAGTGACGGTGGGATTGTCTCCGAGTCAAATCCAGGCGATTAATGAGTCGCCGTTGGACTCGCCAAAGATGTGGATGCAATACATGTGCAGGGCTCATTGCCATGAGGCTCCGTCGTCGTTAGGGGGAGACTTGTGGGACATTGATAATTCCAGTCCAAACAAGTACTACGAAGGTGATGTCGAGTCAATGGACTACGAAGATATGCAGCATTGGTCCATTTGGGACCCCTAA

Protein Analysis

202

Amino Acids

22.33

Weight (kDa)

5.28

Isoelectric Point (pI)

65.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 14 - 63 2.3e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 117
AccII CGCG 2 cut(s) 27, 93
AciI CCGC 7 cut(s) 25, 91, 155, 170, 197, 272, 341
AcoI YGGCCR 1 cut(s) 240
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 1 cut(s) 117
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 1 cut(s) 249
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 3 cut(s) 9, 97, 405
AluBI AGCT 4 cut(s) 129, 152, 310, 366
AluI AGCT 4 cut(s) 129, 152, 310, 366
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 3 cut(s) 34, 396, 495
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 3 cut(s) 156, 236, 240
ApeKI GCWGC 8 cut(s) 14, 149, 152, 167, 284, 307, 310, 580
ArsI GACNNNNNNTTYG 2 cut(s) 537, 569
AseI ATTAAT 1 cut(s) 414
AspLEI GCGC 3 cut(s) 202, 254, 373
AspS9I GGNCC 2 cut(s) 588, 598
AsuHPI GGTGA 2 cut(s) 356, 560
AvaII GGWCC 2 cut(s) 588, 598
BanI GGYRCC 1 cut(s) 136
BanII GRGCYC 2 cut(s) 368, 469
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 3 cut(s) 11, 99, 407
BcoDI GTCTC 3 cut(s) 34, 396, 495
BfaI CTAG 2 cut(s) 122, 269
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 2 cut(s) 203, 374
BlpI GCTNAGC 1 cut(s) 367
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 3 cut(s) 11, 99, 407
Bme18I GGWCC 2 cut(s) 588, 598
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 588, 598
BmiI GGNNCC 4 cut(s) 138, 483, 599, 600
BmrFI CCNGG 3 cut(s) 11, 99, 407
BmsI GCATC 2 cut(s) 154, 438
Bpu1102I GCTNAGC 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 9, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 3 cut(s) 243, 374, 525
Bse3DI GCAATG 3 cut(s) 173, 261, 469
BseBI CCWGG 3 cut(s) 11, 99, 407
BseDI CCNNGG 2 cut(s) 9, 233
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMI GCAATG 3 cut(s) 173, 261, 469
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 3 cut(s) 243, 374, 525
BseRI GAGGAG 2 cut(s) 63, 350
BseXI GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
BsgI GTGCAG 1 cut(s) 481
Bsh1236I CGCG 2 cut(s) 27, 93
BshFI GGCC 3 cut(s) 158, 238, 242
BshNI GGYRCC 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 2 cut(s) 35, 239
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 1 cut(s) 117
BsmAI GTCTC 3 cut(s) 34, 396, 495
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 3 cut(s) 158, 238, 242
Bsp1286I GDGCHC 2 cut(s) 368, 469
Bsp1720I GCTNAGC 1 cut(s) 367
BspACI CCGC 7 cut(s) 25, 91, 155, 170, 197, 272, 341
BspANI GGCC 3 cut(s) 158, 238, 242
BspCNI CTCAG 1 cut(s) 380
BspFNI CGCG 2 cut(s) 27, 93
BspLI GGNNCC 4 cut(s) 138, 483, 599, 600
BspMAI CTGCAG 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 136
BsrDI GCAATG 3 cut(s) 173, 261, 469
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 3 cut(s) 243, 374, 525
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 2 cut(s) 9, 233
Bst2UI CCWGG 3 cut(s) 11, 99, 407
Bst4CI ACNGT 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 59, 240
BstDEI CTNAG 1 cut(s) 367
BstF5I GGATG 1 cut(s) 453
BstFNI CGCG 2 cut(s) 27, 93
BstH2I RGCGCY 2 cut(s) 203, 374
BstHHI GCGC 3 cut(s) 202, 254, 373
BstMAI GTCTC 3 cut(s) 34, 396, 495
BstMWI GCNNNNNNNGC 3 cut(s) 155, 164, 281
BstNI CCWGG 3 cut(s) 11, 99, 407
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 3 cut(s) 9, 97, 405
BstSFI CTRYAG 1 cut(s) 15
BstUI CGCG 2 cut(s) 27, 93
BstV1I GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
BstXI CCANNNNNNTGG 1 cut(s) 444
BsuRI GGCC 3 cut(s) 158, 238, 242
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 381
Cac8I GCNNGC 2 cut(s) 59, 240
CaiI CAGNNNCTG 1 cut(s) 310
CfoI GCGC 3 cut(s) 202, 254, 373
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 2 cut(s) 588, 598
CseI GACGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 538
CspCI CAANNNNNGTGG 2 cut(s) 172, 207
CviAII CATG 2 cut(s) 457, 476
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 1 cut(s) 367
DrdI GACNNNNNNGTC 1 cut(s) 26
DseDI GACNNNNNNGTC 1 cut(s) 26
EaeI YGGCCR 1 cut(s) 240
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 2 cut(s) 368, 469
Eco47I GGWCC 2 cut(s) 588, 598
Eco53kI GAGCTC 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 3 cut(s) 9, 97, 405
EcoT38I GRGCYC 2 cut(s) 368, 469
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 2 cut(s) 460, 479
FaiI YATR 5 cut(s) 118, 162, 458, 477, 578
FaqI GGGAC 1 cut(s) 524
FatI CATG 2 cut(s) 456, 475
FauI CCCGC 1 cut(s) 334
FokI GGATG 1 cut(s) 460
FriOI GRGCYC 2 cut(s) 368, 469
FseI GGCCGGCC 1 cut(s) 242
FspBI CTAG 2 cut(s) 122, 269
GlaI GCGC 3 cut(s) 201, 253, 372
HaeII RGCGCY 2 cut(s) 203, 374
HaeIII GGCC 3 cut(s) 158, 238, 242
HapII CCGG 2 cut(s) 35, 239
HgaI GACGC 1 cut(s) 16
HhaI GCGC 3 cut(s) 202, 254, 373
Hin1II CATG 2 cut(s) 460, 479
Hin6I GCGC 3 cut(s) 200, 252, 371
HinP1I GCGC 3 cut(s) 200, 252, 371
HinfI GANTC 6 cut(s) 5, 347, 397, 419, 431, 557
HpaII CCGG 2 cut(s) 35, 239
HphI GGTGA 2 cut(s) 356, 560
Hpy188I TCNGA 2 cut(s) 85, 396
Hpy188III TCNNGA 2 cut(s) 212, 278
Hpy99I CGWCG 3 cut(s) 355, 490, 493
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 8 cut(s) 17, 167, 178, 265, 287, 451, 462, 580
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 164, 281
HpyF3I CTNAG 1 cut(s) 367
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 2 cut(s) 460, 479
HspAI GCGC 3 cut(s) 200, 252, 371
KflI GGGWCCC 1 cut(s) 598
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 7 cut(s) 139, 161, 179, 271, 297, 319, 592
LweI GCATC 2 cut(s) 154, 438
MaeI CTAG 2 cut(s) 122, 269
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 344, 376
MboII GAAGA 1 cut(s) 584
MhlI GDGCHC 2 cut(s) 368, 469
MluCI AATT 2 cut(s) 205, 520
MlyI GAGTC 5 cut(s) 341, 406, 425, 428, 566
MmeI TCCRAC 2 cut(s) 353, 408
MroNI GCCGGC 1 cut(s) 238
MseI TTAA 2 cut(s) 204, 414
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 3 cut(s) 152, 170, 310
MspI CCGG 2 cut(s) 35, 239
MspR9I CCNGG 3 cut(s) 11, 99, 407
MvaI CCWGG 3 cut(s) 11, 99, 407
MvnI CGCG 2 cut(s) 27, 93
MwoI GCNNNNNNNGC 3 cut(s) 155, 164, 281
NaeI GCCGGC 1 cut(s) 240
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 2 cut(s) 460, 479
NlaIV GGNNCC 4 cut(s) 138, 483, 599, 600
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 2 cut(s) 344, 376
NspI RCATGY 1 cut(s) 460
PciI ACATGT 1 cut(s) 456
PdiI GCCGGC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 5
PflMI CCANNNNNTGG 1 cut(s) 117
PleI GAGTC 5 cut(s) 341, 405, 425, 427, 565
PpsI GAGTC 5 cut(s) 341, 405, 425, 427, 565
PpuMI RGGWCCY 1 cut(s) 598
PscI ACATGT 1 cut(s) 456
PshBI ATTAAT 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 3 cut(s) 9, 97, 405
PspGI CCWGG 3 cut(s) 9, 97, 405
PspN4I GGNNCC 4 cut(s) 138, 483, 599, 600
PspPI GGNCC 2 cut(s) 588, 598
PspPPI RGGWCCY 1 cut(s) 598
PstI CTGCAG 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 310
PvuII CAGCTG 2 cut(s) 152, 310
RigI GGCCGGCC 1 cut(s) 242
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 2 cut(s) 204, 414
Sau96I GGNCC 2 cut(s) 588, 598
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 5 cut(s) 341, 406, 425, 428, 566
ScrFI CCNGG 3 cut(s) 11, 99, 407
SduI GDGCHC 2 cut(s) 368, 469
SetI ASST 7 cut(s) 131, 154, 183, 264, 312, 368, 550
SfaNI GCATC 2 cut(s) 154, 438
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 2 cut(s) 588, 598
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
Sse9I AATT 2 cut(s) 205, 520
SsiI CCGC 7 cut(s) 25, 91, 155, 170, 197, 272, 341
SspMI CTAG 2 cut(s) 122, 269
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 3 cut(s) 9, 97, 405
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 353, 555
TasI AATT 2 cut(s) 205, 520
TatI WGTACW 1 cut(s) 537
TauI GCSGC 5 cut(s) 27, 158, 173, 200, 275
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 204, 414
Tru9I TTAA 2 cut(s) 204, 414
TscAI CASTG 1 cut(s) 381
TseFI GTSAC 2 cut(s) 344, 376
TseI GCWGC 8 cut(s) 14, 149, 152, 167, 284, 307, 310, 580
Tsp45I GTSAC 2 cut(s) 344, 376
TspGWI ACGGA 2 cut(s) 347, 474
TspRI CASTG 1 cut(s) 381
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 2 cut(s) 588, 598
VspI ATTAAT 1 cut(s) 414
XceI RCATGY 1 cut(s) 460
XspI CTAG 2 cut(s) 122, 269
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.