Rroxscaffold_3G00228940
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005624
Physical Location & Seq
Reverse (-)
13438615 .. 13439163
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228940.1

Sequence Viewer

Length: 549 bp
ATGAATGAAGTACACGAACCTGCAGCCAAGTACAGAGGCGTACGCAAGCGAAAATGGGGAAAATGGGTGTCTGAAATCCGCGAGCCGGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCTGAAATGGCTGCTGCGGCCTACGATGCGGCGGCATTGCACCTCAGAGGACCAGGGGTGTTGCTTAACTTTCCTCAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCCACGGACGTGCAGTTGGCGGCTCAAGAGGCGGCAATGAGGTTTAGGAGACTGCCGATGGGGTCGGTTTCGACGGAGGTGGCTGGTGGCTCGAGTGGTGGCGCGGTGCCGGAGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCACCATTGGACTCGCCTAAGATGTGGATGCAGTACATGTACATGTCTCATCGTGATGAGGCTTTGTTTTTTGGGGAGGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGATGGTTTGCAGCATCAGTCCATTTGGGACTCCTAG

Protein Analysis

182

Amino Acids

20.13

Weight (kDa)

5.52

Isoelectric Point (pI)

72.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 10 - 59 1.7e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 346
AccII CGCG 2 cut(s) 81, 344
AciI CCGC 7 cut(s) 79, 143, 155, 158, 260, 272, 344
AcsI RAATTY 1 cut(s) 378
AfaI GTAC 5 cut(s) 12, 32, 42, 431, 437
AfiI CCNNNNNNNGG 3 cut(s) 85, 105, 133
AflIII ACRYGT 2 cut(s) 432, 438
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 178
AleI CACNNNNGTG 1 cut(s) 248
AluBI AGCT 1 cut(s) 117
AluI AGCT 1 cut(s) 117
Alw26I GTCTC 3 cut(s) 283, 347, 447
Ama87I CYCGRG 1 cut(s) 331
AoxI GGCC 3 cut(s) 123, 144, 224
ApeKI GCWGC 4 cut(s) 23, 137, 140, 523
ApoI RAATTY 1 cut(s) 378
AseI ATTAAT 1 cut(s) 390
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 2 cut(s) 124, 176
AsuC2I CCSGG 2 cut(s) 87, 100
AsuHPI GGTGA 1 cut(s) 390
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 4 cut(s) 35, 124, 127, 535
BccI CCATC 3 cut(s) 292, 490, 509
BciT130I CCWGG 1 cut(s) 180
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 2 cut(s) 87, 100
BcoDI GTCTC 3 cut(s) 283, 347, 447
BfaI CTAG 2 cut(s) 110, 547
BfmI CTRYAG 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 28
BisI GCNGC 9 cut(s) 24, 138, 141, 144, 156, 159, 261, 273, 524
BlsI GCNGC 9 cut(s) 25, 139, 142, 145, 157, 160, 262, 274, 525
Bme1390I CCNGG 3 cut(s) 87, 100, 180
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 124, 176
BmiI GGNNCC 2 cut(s) 126, 348
BmrFI CCNGG 3 cut(s) 87, 100, 180
BmsI GCATC 3 cut(s) 142, 414, 535
BpiI GAAGAC 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 249
BpuMI CCSGG 2 cut(s) 87, 100
BsaJI CCNNGG 3 cut(s) 179, 221, 243
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 105, 133
Bse3DI GCAATG 2 cut(s) 161, 282
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 3 cut(s) 179, 221, 243
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 85, 105, 133
BseMI GCAATG 2 cut(s) 161, 282
BseMII CTCAG 1 cut(s) 184
BseXI GCAGC 4 cut(s) 35, 124, 127, 535
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 2 cut(s) 81, 344
BshFI GGCC 3 cut(s) 125, 146, 226
BshNI GGYRCC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 3 cut(s) 86, 100, 350
BsiWI CGTACG 1 cut(s) 40
BslFI GGGAC 2 cut(s) 225, 375
BslI CCNNNNNNNGG 3 cut(s) 85, 105, 133
BsmAI GTCTC 3 cut(s) 283, 347, 447
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 2 cut(s) 225, 375
BsnI GGCC 3 cut(s) 125, 146, 226
BsoBI CYCGRG 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 1 cut(s) 505
BspACI CCGC 7 cut(s) 79, 143, 155, 158, 260, 272, 344
BspANI GGCC 3 cut(s) 125, 146, 226
BspCNI CTCAG 1 cut(s) 183
BspFNI CGCG 2 cut(s) 81, 344
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 126, 348
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 161, 282
BsrGI TGTACA 1 cut(s) 435
BssECI CCNNGG 3 cut(s) 179, 221, 243
BssMI GATC 1 cut(s) 505
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 2 cut(s) 358, 366
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 47, 83, 228
BstDEI CTNAG 2 cut(s) 170, 414
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 2 cut(s) 81, 344
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 1 cut(s) 508
BstMAI GTCTC 3 cut(s) 283, 347, 447
BstMBI GATC 1 cut(s) 505
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 269
BstNI CCWGG 1 cut(s) 180
BstNSI RCATGY 3 cut(s) 436, 442, 481
BstSCI CCNGG 3 cut(s) 85, 98, 178
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 2 cut(s) 81, 344
BstV1I GCAGC 4 cut(s) 35, 124, 127, 535
BstV2I GAAGAC 1 cut(s) 209
BsuRI GGCC 3 cut(s) 125, 146, 226
BtgI CCRYGG 1 cut(s) 243
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 3 cut(s) 47, 83, 228
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 2 cut(s) 124, 176
Csp6I GTAC 5 cut(s) 11, 31, 41, 430, 436
CviAII CATG 4 cut(s) 433, 439, 478, 509
CviQI GTAC 5 cut(s) 11, 31, 41, 430, 436
DdeI CTNAG 2 cut(s) 170, 414
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 4 cut(s) 436, 442, 481, 512
FaiI YATR 7 cut(s) 106, 434, 440, 479, 491, 501, 510
FaqI GGGAC 2 cut(s) 225, 375
FatI CATG 4 cut(s) 432, 438, 477, 508
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 9 cut(s) 24, 138, 141, 144, 156, 159, 261, 273, 524
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 9 cut(s) 24, 138, 141, 144, 156, 159, 261, 273, 524
FspBI CTAG 2 cut(s) 110, 547
GlaI GCGC 1 cut(s) 343
GluI GCNGC 9 cut(s) 24, 138, 141, 144, 156, 159, 261, 273, 524
HaeIII GGCC 3 cut(s) 125, 146, 226
HapII CCGG 3 cut(s) 86, 100, 350
HhaI GCGC 1 cut(s) 344
Hin1II CATG 4 cut(s) 436, 442, 481, 512
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 5 cut(s) 238, 373, 395, 407, 542
HpaII CCGG 3 cut(s) 86, 100, 350
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 3 cut(s) 73, 173, 222
Hpy188III TCNNGA 3 cut(s) 266, 449, 509
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 358, 366
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 5 cut(s) 23, 166, 253, 427, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 269
HpyF3I CTNAG 2 cut(s) 170, 414
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 4 cut(s) 436, 442, 481, 512
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 1 cut(s) 505
Lsp1109I GCAGC 4 cut(s) 35, 124, 127, 535
LweI GCATC 3 cut(s) 142, 414, 535
MaeI CTAG 2 cut(s) 110, 547
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 396
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MboII GAAGA 1 cut(s) 209
MluCI AATT 1 cut(s) 378
MlyI GAGTC 5 cut(s) 247, 382, 401, 404, 536
MseI TTAA 2 cut(s) 192, 390
MslI CAYNNNNRTG 4 cut(s) 248, 437, 450, 513
MspI CCGG 3 cut(s) 86, 100, 350
MspR9I CCNGG 3 cut(s) 87, 100, 180
MvaI CCWGG 1 cut(s) 180
MvnI CGCG 2 cut(s) 81, 344
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 269
NciI CCSGG 2 cut(s) 87, 100
NdeII GATC 1 cut(s) 505
NlaIII CATG 4 cut(s) 436, 442, 481, 512
NlaIV GGNNCC 2 cut(s) 126, 348
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 396
NspI RCATGY 3 cut(s) 436, 442, 481
OliI CACNNNNGTG 1 cut(s) 248
PaeR7I CTCGAG 1 cut(s) 331
PagI TCATGA 1 cut(s) 508
PciI ACATGT 2 cut(s) 432, 438
Pfl23II CGTACG 1 cut(s) 40
PkrI GCNGC 9 cut(s) 25, 139, 142, 145, 157, 160, 262, 274, 525
PleI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PpsI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PscI ACATGT 2 cut(s) 432, 438
PshBI ATTAAT 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspLI CGTACG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 126, 348
PspPI GGNCC 2 cut(s) 124, 176
PspXI VCTCGAGB 1 cut(s) 331
PstI CTGCAG 1 cut(s) 25
RsaI GTAC 5 cut(s) 12, 32, 42, 431, 437
RsaNI GTAC 5 cut(s) 11, 31, 41, 430, 436
RseI CAYNNNNRTG 4 cut(s) 248, 437, 450, 513
SaqAI TTAA 2 cut(s) 192, 390
SatI GCNGC 9 cut(s) 24, 138, 141, 144, 156, 159, 261, 273, 524
Sau3AI GATC 1 cut(s) 505
Sau96I GGNCC 2 cut(s) 124, 176
SchI GAGTC 5 cut(s) 247, 382, 401, 404, 536
ScrFI CCNGG 3 cut(s) 87, 100, 180
SetI ASST 6 cut(s) 22, 119, 171, 252, 284, 321
SfaNI GCATC 3 cut(s) 142, 414, 535
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 331
SinI GGWCC 1 cut(s) 176
SlaI CTCGAG 1 cut(s) 331
SmiMI CAYNNNNRTG 4 cut(s) 248, 437, 450, 513
SmlI CTYRAG 2 cut(s) 264, 331
SmoI CTYRAG 2 cut(s) 264, 331
Sse9I AATT 1 cut(s) 378
SsiI CCGC 7 cut(s) 79, 143, 155, 158, 260, 272, 344
SspMI CTAG 2 cut(s) 110, 547
StyD4I CCNGG 3 cut(s) 85, 98, 178
TaaI ACNGT 2 cut(s) 358, 366
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 236, 311, 332
TasI AATT 1 cut(s) 378
TatI WGTACW 4 cut(s) 10, 30, 429, 435
TauI GCSGC 5 cut(s) 146, 158, 161, 263, 275
Tru1I TTAA 2 cut(s) 192, 390
Tru9I TTAA 2 cut(s) 192, 390
TseFI GTSAC 1 cut(s) 396
TseI GCWGC 4 cut(s) 23, 137, 140, 523
Tsp45I GTSAC 1 cut(s) 396
TspDTI ATGAA 2 cut(s) 17, 21
TspGWI ACGGA 2 cut(s) 260, 329
VpaK11BI GGWCC 1 cut(s) 176
VspI ATTAAT 1 cut(s) 390
XapI RAATTY 1 cut(s) 378
XceI RCATGY 3 cut(s) 436, 442, 481
XhoI CTCGAG 1 cut(s) 331
XspI CTAG 2 cut(s) 110, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.