Rw7G034830
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005624
Physical Location & Seq
Forward (+)
51815248 .. 51815836
589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G034830.1

Sequence Viewer

Length: 549 bp
ATGGAGGAAGCACACGAGCCTGCAGTCAAGTACAGAGGCGTACGCAAGCGAAAATGGGGTAAATGGGTGTCCGAAATCCGGGAACCGGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCCGAAATGGCCGCCGCCGCCTACGATGCGGCGGCATTTCACCTCAGAGGACCAGAGGTCCTGTTTAACTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCTTCGGACGTGCAGTTGGCGGCCCAAGGGGCGGCAATGAGGTTTAGGAGACTGCCAATGGGGTCGGTTTCGACGGAGGTGGCTGGTGGCTCGAGTGGTGGCACGGTGCCGGTGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCGCCATTGGACTCGCCTAAGATGTGGATGCAGTACATGTACATGTCTCATCGTGATGAGGCTTTGTTTTTTGGGGAGGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGACGGTTTGCAGCATCAGTCCATTTGGGACTCCTAG

Protein Analysis

182

Amino Acids

20.2

Weight (kDa)

5.35

Isoelectric Point (pI)

73.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 10 - 59 1.5e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AciI CCGC 7 cut(s) 138, 141, 144, 155, 158, 260, 272
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 378
AfaI GTAC 4 cut(s) 32, 42, 431, 437
AfiI CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 271
AflIII ACRYGT 2 cut(s) 432, 438
AhdI GACNNNNNGTC 1 cut(s) 182
AjiI CACGTC 1 cut(s) 250
AluBI AGCT 1 cut(s) 117
AluI AGCT 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 283, 447
Ama87I CYCGRG 1 cut(s) 331
AoxI GGCC 4 cut(s) 123, 135, 224, 261
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 1 cut(s) 378
AseI ATTAAT 1 cut(s) 390
AspS9I GGNCC 4 cut(s) 124, 176, 184, 262
AsuC2I CCSGG 3 cut(s) 80, 87, 100
AsuHPI GGTGA 2 cut(s) 158, 364
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 2 cut(s) 176, 184
BanI GGYRCC 1 cut(s) 346
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 535
BccI CCATC 1 cut(s) 490
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 3 cut(s) 80, 87, 100
BcoDI GTCTC 2 cut(s) 283, 447
BfaI CTAG 2 cut(s) 110, 547
BfmI CTRYAG 1 cut(s) 21
BglI GCCNNNNNGGC 1 cut(s) 269
BisI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 524
BlsI GCNGC 8 cut(s) 139, 142, 145, 157, 160, 262, 274, 525
Bme1390I CCNGG 3 cut(s) 80, 87, 100
Bme18I GGWCC 2 cut(s) 176, 184
BmeRI GACNNNNNGTC 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 4 cut(s) 124, 176, 184, 262
BmiI GGNNCC 3 cut(s) 84, 126, 348
BmrFI CCNGG 3 cut(s) 80, 87, 100
BmsI GCATC 3 cut(s) 142, 414, 535
BpiI GAAGAC 1 cut(s) 209
BpuMI CCSGG 3 cut(s) 80, 87, 100
BsaJI CCNNGG 2 cut(s) 221, 265
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 271
Bse118I RCCGGY 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 282
BseDI CCNNGG 2 cut(s) 221, 265
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 271
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 184
BseXI GCAGC 1 cut(s) 535
BsgI GTGCAG 1 cut(s) 272
BshFI GGCC 4 cut(s) 125, 137, 226, 263
BshNI GGYRCC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 4 cut(s) 79, 86, 100, 350
BsiWI CGTACG 1 cut(s) 40
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 5 cut(s) 78, 85, 105, 133, 271
BsmAI GTCTC 2 cut(s) 283, 447
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 4 cut(s) 125, 137, 226, 263
BsoBI CYCGRG 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 1 cut(s) 505
BspACI CCGC 7 cut(s) 138, 141, 144, 155, 158, 260, 272
BspANI GGCC 4 cut(s) 125, 137, 226, 263
BspCNI CTCAG 1 cut(s) 183
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 84, 126, 348
BspMAI CTGCAG 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 349
BsrGI TGTACA 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 221, 265
BssMI GATC 1 cut(s) 505
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 14
Bst4CI ACNGT 4 cut(s) 346, 358, 366, 518
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 21, 47, 228
BstDEI CTNAG 2 cut(s) 170, 414
BstF5I GGATG 1 cut(s) 429
BstKTI GATC 1 cut(s) 508
BstMAI GTCTC 2 cut(s) 283, 447
BstMBI GATC 1 cut(s) 505
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 269
BstNSI RCATGY 3 cut(s) 436, 442, 481
BstSCI CCNGG 3 cut(s) 78, 85, 98
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 209
BsuRI GGCC 4 cut(s) 125, 137, 226, 263
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 429
Cac8I GCNNGC 3 cut(s) 21, 47, 228
CciI TCATGA 1 cut(s) 508
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 4 cut(s) 124, 176, 184, 262
Csp6I GTAC 4 cut(s) 31, 41, 430, 436
CviAII CATG 4 cut(s) 433, 439, 478, 509
CviQI GTAC 4 cut(s) 31, 41, 430, 436
DdeI CTNAG 2 cut(s) 170, 414
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
DriI GACNNNNNGTC 1 cut(s) 182
EaeI YGGCCR 1 cut(s) 135
Eam1105I GACNNNNNGTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 176, 184
Eco88I CYCGRG 1 cut(s) 331
EcoO109I RGGNCCY 2 cut(s) 124, 184
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 4 cut(s) 436, 442, 481, 512
FaiI YATR 7 cut(s) 106, 434, 440, 479, 491, 501, 510
FaqI GGGAC 1 cut(s) 375
FatI CATG 4 cut(s) 432, 438, 477, 508
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 524
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 524
FspBI CTAG 2 cut(s) 110, 547
GluI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 524
HaeIII GGCC 4 cut(s) 125, 137, 226, 263
HapII CCGG 4 cut(s) 79, 86, 100, 350
Hin1II CATG 4 cut(s) 436, 442, 481, 512
HinfI GANTC 5 cut(s) 238, 373, 395, 407, 542
HpaII CCGG 4 cut(s) 79, 86, 100, 350
HphI GGTGA 2 cut(s) 158, 364
Hpy188I TCNGA 4 cut(s) 73, 173, 222, 247
Hpy188III TCNNGA 3 cut(s) 200, 449, 509
Hpy99I CGWCG 1 cut(s) 316
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 4 cut(s) 346, 358, 366, 518
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 4 cut(s) 23, 253, 427, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 269
HpyF3I CTNAG 2 cut(s) 170, 414
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 4 cut(s) 436, 442, 481, 512
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 1 cut(s) 505
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 3 cut(s) 142, 414, 535
MaeI CTAG 2 cut(s) 110, 547
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 352
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MboII GAAGA 1 cut(s) 209
MluCI AATT 1 cut(s) 378
MlyI GAGTC 5 cut(s) 247, 382, 401, 404, 536
MseI TTAA 2 cut(s) 192, 390
MslI CAYNNNNRTG 2 cut(s) 437, 450
MspI CCGG 4 cut(s) 79, 86, 100, 350
MspR9I CCNGG 3 cut(s) 80, 87, 100
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 269
NciI CCSGG 3 cut(s) 80, 87, 100
NdeII GATC 1 cut(s) 505
NlaIII CATG 4 cut(s) 436, 442, 481, 512
NlaIV GGNNCC 3 cut(s) 84, 126, 348
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 352
NspI RCATGY 3 cut(s) 436, 442, 481
PaeR7I CTCGAG 1 cut(s) 331
PagI TCATGA 1 cut(s) 508
PciI ACATGT 2 cut(s) 432, 438
PcsI WCGNNNNNNNCGW 1 cut(s) 126
Pfl23II CGTACG 1 cut(s) 40
PfoI TCCNGGA 1 cut(s) 78
PkrI GCNGC 8 cut(s) 139, 142, 145, 157, 160, 262, 274, 525
PleI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PpsI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PpuMI RGGWCCY 1 cut(s) 184
PscI ACATGT 2 cut(s) 432, 438
PshBI ATTAAT 1 cut(s) 390
Psp5II RGGWCCY 1 cut(s) 184
PspLI CGTACG 1 cut(s) 40
PspN4I GGNNCC 3 cut(s) 84, 126, 348
PspPI GGNCC 4 cut(s) 124, 176, 184, 262
PspPPI RGGWCCY 1 cut(s) 184
PspXI VCTCGAGB 1 cut(s) 331
PstI CTGCAG 1 cut(s) 25
RsaI GTAC 4 cut(s) 32, 42, 431, 437
RsaNI GTAC 4 cut(s) 31, 41, 430, 436
RseI CAYNNNNRTG 2 cut(s) 437, 450
SaqAI TTAA 2 cut(s) 192, 390
SatI GCNGC 8 cut(s) 138, 141, 144, 156, 159, 261, 273, 524
Sau3AI GATC 1 cut(s) 505
Sau96I GGNCC 4 cut(s) 124, 176, 184, 262
SchI GAGTC 5 cut(s) 247, 382, 401, 404, 536
ScrFI CCNGG 3 cut(s) 80, 87, 100
SetI ASST 6 cut(s) 119, 171, 186, 252, 284, 321
SfaNI GCATC 3 cut(s) 142, 414, 535
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 331
SinI GGWCC 2 cut(s) 176, 184
SlaI CTCGAG 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 437, 450
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
Sse9I AATT 1 cut(s) 378
SsiI CCGC 7 cut(s) 138, 141, 144, 155, 158, 260, 272
SspMI CTAG 2 cut(s) 110, 547
StyD4I CCNGG 3 cut(s) 78, 85, 98
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 4 cut(s) 346, 358, 366, 518
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 236, 311, 332
TasI AATT 1 cut(s) 378
TatI WGTACW 3 cut(s) 30, 429, 435
TauI GCSGC 7 cut(s) 140, 143, 146, 158, 161, 263, 275
Tru1I TTAA 2 cut(s) 192, 390
Tru9I TTAA 2 cut(s) 192, 390
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 523
Tsp45I GTSAC 1 cut(s) 352
TspGWI ACGGA 1 cut(s) 329
VpaK11BI GGWCC 2 cut(s) 176, 184
VspI ATTAAT 1 cut(s) 390
XapI RAATTY 1 cut(s) 378
XceI RCATGY 3 cut(s) 436, 442, 481
XhoI CTCGAG 1 cut(s) 331
XspI CTAG 2 cut(s) 110, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.