Rh7CG442700
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0005624
Physical Location & Seq
Forward (+)
58067846 .. 58068394
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG442700.1

Sequence Viewer

Length: 549 bp
ATGGAGGAAGTACACGAGCCTGCAGTCAAGTACAGAGGCGTACGCAAGCGAAAATGGGGTAAATGGGTGTCCGAAATCAGGGAACCGGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCCGAAATGGCCGCCGCCGCCTACGATGCGGCGGCATTGCACCTCAGAGGACCGGGGGTCCTGCTTAATTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCAGCGAGTTCGAGTCCCTCGGACGTGCAGTTGGCGGCTCAAGAGGCGGCTATGAGGTTTAGGAGACTGCCAATGGGGTCGGTTTCGACGGAGGTGGCTGGTGGCTCCAGTGGTGGCGCGGCGCCGGTGACGGTGGGACTGTCGCCGAGTCAAATTCAGGCGATTAATGAGTCGCCATTAGACTCACCTAAGATGTGGATGCAGTACATGTACATGTCTCATCGTGATGAGGCTTTGTTTTTTGGGGAGGACATGCCATCGTCTATGGGTTTCTATGATGATCATGATGATGGTTTGCAGCATCAGTCCATTTGGGACTCCTAG

Protein Analysis

182

Amino Acids

20.1

Weight (kDa)

5.35

Isoelectric Point (pI)

74.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 10 - 59 1.2e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccII CGCG 1 cut(s) 344
AciI CCGC 8 cut(s) 138, 141, 144, 155, 158, 260, 272, 344
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 347
AfaI GTAC 5 cut(s) 12, 32, 42, 431, 437
AfiI CCNNNNNNNGG 3 cut(s) 78, 105, 133
AflIII ACRYGT 2 cut(s) 432, 438
AhdI GACNNNNNGTC 1 cut(s) 182
AjiI CACGTC 1 cut(s) 250
AluBI AGCT 1 cut(s) 117
AluI AGCT 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 283, 447
AoxI GGCC 3 cut(s) 123, 135, 224
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 1 cut(s) 378
AseI ATTAAT 1 cut(s) 390
AspLEI GCGC 2 cut(s) 344, 349
AspS9I GGNCC 3 cut(s) 124, 176, 184
AsuC2I CCSGG 3 cut(s) 87, 100, 180
AsuHPI GGTGA 2 cut(s) 364, 402
AvaII GGWCC 2 cut(s) 176, 184
BanI GGYRCC 1 cut(s) 346
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 535
BccI CCATC 2 cut(s) 490, 509
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 3 cut(s) 87, 100, 180
BcoDI GTCTC 2 cut(s) 283, 447
BfaI CTAG 2 cut(s) 110, 547
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 350
BisI GCNGC 9 cut(s) 138, 141, 144, 156, 159, 261, 273, 345, 524
BlsI GCNGC 9 cut(s) 139, 142, 145, 157, 160, 262, 274, 346, 525
Bme1390I CCNGG 3 cut(s) 87, 100, 180
Bme18I GGWCC 2 cut(s) 176, 184
BmeRI GACNNNNNGTC 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 3 cut(s) 124, 176, 184
BmiI GGNNCC 5 cut(s) 84, 126, 185, 331, 348
BmrFI CCNGG 3 cut(s) 87, 100, 180
BmsI GCATC 3 cut(s) 142, 414, 535
BpiI GAAGAC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 316
BpuEI CTTGAG 1 cut(s) 249
BpuMI CCSGG 3 cut(s) 87, 100, 180
BsaHI GRCGYC 1 cut(s) 347
BsaJI CCNNGG 3 cut(s) 179, 221, 243
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 105, 133
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 161
BseDI CCNNGG 3 cut(s) 179, 221, 243
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 78, 105, 133
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 1 cut(s) 333
BseXI GCAGC 1 cut(s) 535
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 3 cut(s) 125, 137, 226
BshNI GGYRCC 1 cut(s) 346
BsiSI CCGG 4 cut(s) 86, 100, 179, 350
BsiWI CGTACG 1 cut(s) 40
BslFI GGGAC 2 cut(s) 225, 375
BslI CCNNNNNNNGG 3 cut(s) 78, 105, 133
BsmAI GTCTC 2 cut(s) 283, 447
BsmFI GGGAC 2 cut(s) 225, 375
BsnI GGCC 3 cut(s) 125, 137, 226
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 1 cut(s) 505
BspACI CCGC 8 cut(s) 138, 141, 144, 155, 158, 260, 272, 344
BspANI GGCC 3 cut(s) 125, 137, 226
BspCNI CTCAG 1 cut(s) 183
BspFNI CGCG 1 cut(s) 344
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 5 cut(s) 84, 126, 185, 331, 348
BspMAI CTGCAG 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 349
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 3 cut(s) 179, 221, 243
BssMI GATC 1 cut(s) 505
BssNI GRCGYC 1 cut(s) 347
BssSI CACGAG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 14
Bst4CI ACNGT 2 cut(s) 358, 366
BstACI GRCGYC 1 cut(s) 347
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 21, 47, 228
BstDEI CTNAG 2 cut(s) 170, 414
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 1 cut(s) 344
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 2 cut(s) 344, 349
BstKTI GATC 1 cut(s) 508
BstMAI GTCTC 2 cut(s) 283, 447
BstMBI GATC 1 cut(s) 505
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 269
BstNSI RCATGY 3 cut(s) 436, 442, 481
BstSCI CCNGG 3 cut(s) 85, 98, 178
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 209
BsuRI GGCC 3 cut(s) 125, 137, 226
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 3 cut(s) 21, 47, 228
CciI TCATGA 1 cut(s) 508
CfoI GCGC 2 cut(s) 344, 349
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 3 cut(s) 124, 176, 184
Csp6I GTAC 5 cut(s) 11, 31, 41, 430, 436
CviAII CATG 4 cut(s) 433, 439, 478, 509
CviQI GTAC 5 cut(s) 11, 31, 41, 430, 436
DdeI CTNAG 2 cut(s) 170, 414
DinI GGCGCC 1 cut(s) 348
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
DriI GACNNNNNGTC 1 cut(s) 182
EaeI YGGCCR 1 cut(s) 135
Eam1105I GACNNNNNGTC 1 cut(s) 182
Eco47I GGWCC 2 cut(s) 176, 184
EcoO109I RGGNCCY 2 cut(s) 124, 184
EgeI GGCGCC 1 cut(s) 348
EheI GGCGCC 1 cut(s) 348
FaeI CATG 4 cut(s) 436, 442, 481, 512
FaiI YATR 8 cut(s) 106, 278, 434, 440, 479, 491, 501, 510
FaqI GGGAC 2 cut(s) 225, 375
FatI CATG 4 cut(s) 432, 438, 477, 508
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 9 cut(s) 138, 141, 144, 156, 159, 261, 273, 345, 524
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 9 cut(s) 138, 141, 144, 156, 159, 261, 273, 345, 524
FspBI CTAG 2 cut(s) 110, 547
GlaI GCGC 2 cut(s) 343, 348
GluI GCNGC 9 cut(s) 138, 141, 144, 156, 159, 261, 273, 345, 524
GsuI CTGGAG 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 3 cut(s) 125, 137, 226
HapII CCGG 4 cut(s) 86, 100, 179, 350
HhaI GCGC 2 cut(s) 344, 349
Hin1I GRCGYC 1 cut(s) 347
Hin1II CATG 4 cut(s) 436, 442, 481, 512
Hin6I GCGC 2 cut(s) 342, 347
HinP1I GCGC 2 cut(s) 342, 347
HinfI GANTC 5 cut(s) 238, 373, 395, 407, 542
HpaII CCGG 4 cut(s) 86, 100, 179, 350
HphI GGTGA 2 cut(s) 364, 402
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 4 cut(s) 73, 173, 222, 247
Hpy188III TCNNGA 4 cut(s) 200, 266, 449, 509
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 358, 366
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 5 cut(s) 23, 166, 253, 427, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 269
HpyF3I CTNAG 2 cut(s) 170, 414
HpySE526I ACGT 1 cut(s) 249
Hsp92I GRCGYC 1 cut(s) 347
Hsp92II CATG 4 cut(s) 436, 442, 481, 512
HspAI GCGC 2 cut(s) 342, 347
KasI GGCGCC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 1 cut(s) 505
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 3 cut(s) 142, 414, 535
MaeI CTAG 2 cut(s) 110, 547
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 352
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MboII GAAGA 1 cut(s) 209
MluCI AATT 2 cut(s) 193, 378
Mly113I GGCGCC 1 cut(s) 347
MlyI GAGTC 5 cut(s) 247, 382, 401, 404, 536
MseI TTAA 2 cut(s) 192, 390
MslI CAYNNNNRTG 3 cut(s) 437, 450, 513
MspI CCGG 4 cut(s) 86, 100, 179, 350
MspR9I CCNGG 3 cut(s) 87, 100, 180
MteI GCGCNGCGC 1 cut(s) 345
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 269
NarI GGCGCC 1 cut(s) 347
NciI CCSGG 3 cut(s) 87, 100, 180
NdeII GATC 1 cut(s) 505
NlaIII CATG 4 cut(s) 436, 442, 481, 512
NlaIV GGNNCC 5 cut(s) 84, 126, 185, 331, 348
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 352
NspI RCATGY 3 cut(s) 436, 442, 481
PagI TCATGA 1 cut(s) 508
PciI ACATGT 2 cut(s) 432, 438
PcsI WCGNNNNNNNCGW 1 cut(s) 126
Pfl23II CGTACG 1 cut(s) 40
PkrI GCNGC 9 cut(s) 139, 142, 145, 157, 160, 262, 274, 346, 525
PleI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PluTI GGCGCC 1 cut(s) 350
PpsI GAGTC 5 cut(s) 246, 381, 401, 403, 536
PpuMI RGGWCCY 1 cut(s) 184
PscI ACATGT 2 cut(s) 432, 438
PshBI ATTAAT 1 cut(s) 390
Psp5II RGGWCCY 1 cut(s) 184
PspLI CGTACG 1 cut(s) 40
PspN4I GGNNCC 5 cut(s) 84, 126, 185, 331, 348
PspPI GGNCC 3 cut(s) 124, 176, 184
PspPPI RGGWCCY 1 cut(s) 184
PstI CTGCAG 1 cut(s) 25
RsaI GTAC 5 cut(s) 12, 32, 42, 431, 437
RsaNI GTAC 5 cut(s) 11, 31, 41, 430, 436
RseI CAYNNNNRTG 3 cut(s) 437, 450, 513
SaqAI TTAA 2 cut(s) 192, 390
SatI GCNGC 9 cut(s) 138, 141, 144, 156, 159, 261, 273, 345, 524
Sau3AI GATC 1 cut(s) 505
Sau96I GGNCC 3 cut(s) 124, 176, 184
SchI GAGTC 5 cut(s) 247, 382, 401, 404, 536
ScrFI CCNGG 3 cut(s) 87, 100, 180
SetI ASST 6 cut(s) 119, 171, 252, 284, 321, 415
SfaNI GCATC 3 cut(s) 142, 414, 535
SfcI CTRYAG 1 cut(s) 21
SfoI GGCGCC 1 cut(s) 348
SgrAI CRCCGGYG 1 cut(s) 349
SinI GGWCC 2 cut(s) 176, 184
SmiMI CAYNNNNRTG 3 cut(s) 437, 450, 513
SmlI CTYRAG 1 cut(s) 264
SmoI CTYRAG 1 cut(s) 264
Sse9I AATT 2 cut(s) 193, 378
SsiI CCGC 8 cut(s) 138, 141, 144, 155, 158, 260, 272, 344
SspDI GGCGCC 1 cut(s) 346
SspMI CTAG 2 cut(s) 110, 547
StyD4I CCNGG 3 cut(s) 85, 98, 178
TaaI ACNGT 2 cut(s) 358, 366
TaiI ACGT 1 cut(s) 252
TaqI TCGA 2 cut(s) 236, 311
TasI AATT 2 cut(s) 193, 378
TatI WGTACW 4 cut(s) 10, 30, 429, 435
TauI GCSGC 8 cut(s) 140, 143, 146, 158, 161, 263, 275, 347
Tru1I TTAA 2 cut(s) 192, 390
Tru9I TTAA 2 cut(s) 192, 390
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 523
Tsp45I GTSAC 1 cut(s) 352
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 176, 184
VspI ATTAAT 1 cut(s) 390
XapI RAATTY 1 cut(s) 378
XceI RCATGY 3 cut(s) 436, 442, 481
XspI CTAG 2 cut(s) 110, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.