Rorug07G0267800
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005624
Physical Location & Seq
Reverse (-)
25258345 .. 25264094
5750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0267800.1

Sequence Viewer

Length: 1803 bp
ATGAAGTATAATAAAGAAGCAAAACCGGGTGCGCAGGAGAAAAGCTCCAGGGTTTTACAGTTGGCAGTACCCTTTTTCTTGCTATGCGGGTTTTCATTCTATCTTGGTGGAATCTTCTGCTCTGAGAAGACCAGATTGGAGACTAATGGTGTCAAGATCGCAGTTCCATCTGCTAAGGAGTCACTGGCGGCCCCTCTCCAGATAAAAGCTGTGTCCTTCCCTGAATGCAGTGCCGACTATCAAGACTACACTCCTTGCACAGATCCAAGGAGGTGGAGGAAGTATGGTGCTCATCGGCTTACCTTCATGGAACGCCACTGCCCTCCAGTATTTGAAAGGAAAGAATGCCTAGTTCCACCTCCAGATGGATACAAGTCTCCAATCAAGTGGCCAAATAGCCGGGATAAATGTTGGTACAGGAATGTGCCATATGATTGGATTAACAAGCAGAAATCTAATCAGAATTGGCTGAGAAAAGAAGGAGACAAGTTTCTTTTTCCTGGTGGGGGAACTATGTTTCCTAAAGGTGTTTCTGCATATGTTGATTTAATGCAAGATCTTATTCCAGAAATGAAAGATGGCACTATCCGAACTGCCATCGACACTGGTTGTGGGGTTGCAAGTTGGGGAGGTGATTTGTTAGATCGTGGGATTTTAACTGTATCTCTTGCACCAAGAGATAATCATGAAGCTCAAGTTCAGTTCGCATTGGAACGTGGAATTCCAGCAGTCCTTGGCATCATTTCCACTCAGAGGCTTCCTTTCCCCTCAAAGTCATTTGATATGGCTCACTGTTCTAGATGCCTTATCCCATGGACAGAATTCGGTGGAATTTACCTGCTAGAAGTTCATCGCATTCTTCGTCCTGGAGGTTTCTGGGTCCTCTCTGGTCCACCTGTCAACTATGAAAACCGCTGGCGTGGATGGAACACAACTATAGAAGACCAGAAAACAGATTACGAGAAGTTGCAAGATCTGCTAACTTCATTGTGCTTCAAATTGTACAACAAAAAGGATGACATTGCTGTTTGGCAGAAGTTGTCAGACAATAGTTGCTATGATAAGCTCTCTGAACCAGATACCTATCCTGCAAAGTGTGATGATAGTCTTGAACCAGATTCAGCATGGTACACTCCATTGCGTTCTTGTGTTGTCGTTCCAAACCCAAAGCACAAGAGGTCGTCTTTGGACACCATCCCTAAATGGCCAGAGCGGTTGCATGTTGCACCAGAACGTATTTCTGATGTTCATGGTGGGAGTGCTAGTGCTCTGAAGCATGATGATGGCAAGTGGAAGATACGATTGCAGCACTACAAGAAGTTGCTCCCAGCAGTTGGAACAGATAAGATCAGAAATGTTATGGACATGAATACCGTGTATGGAGGGCTTGCAGCAGCCTCAATTGATGATCCCTTGTGGGTCATGAATGTGGTTTCTTCCTACGCTGCAAATACACTGCCTGTGGTCTATGATCGGGGCCTTATTGGAACATACCATGACTGGTGCGAAGCTTTCTCTACATATCCCCGAACTTATGATCTTCTACATCTTGATGGCCTCTTTACTGCAGAAAGCCACAGATGCGAAATGAAATACGTGCTCCTAGAGATGGACCGCATCCTACGCCCCAATGGCTATGCAATAATTCGGGAATCCAGCTACTTTGTGGATGCTGTCGCAACTATTGCCAAAGGGATGAGATGGGGATGTCGCAAAGAAGATACCGAGTATAGTGTTGAGAAGGAGAAAATATTGATCTGCCAGAAAAAGCTTTGGTATTCATCTAACCAGAGTTCTAGATGA

Protein Analysis

600

Amino Acids

68.46

Weight (kDa)

8.54

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 82 - 589 3.4e-215 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 846
AccB7I CCANNNNNTGG 1 cut(s) 1334
AccBSI CCGCTC 1 cut(s) 1213
AciI CCGC 5 cut(s) 87, 188, 913, 1213, 1615
AclWI GGATC 2 cut(s) 257, 1403
AcoI YGGCCR 2 cut(s) 389, 1205
AcsI RAATTY 3 cut(s) 720, 821, 831
AcuI CTGAAG 1 cut(s) 1292
AfaI GTAC 4 cut(s) 69, 416, 1004, 1130
AfiI CCNNNNNNNGG 5 cut(s) 365, 506, 753, 1334, 1609
AgsI TTSAA 3 cut(s) 335, 997, 1112
AjnI CCWGG 3 cut(s) 47, 499, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
AluBI AGCT 7 cut(s) 45, 209, 692, 1066, 1511, 1659, 1771
AluI AGCT 7 cut(s) 45, 209, 692, 1066, 1511, 1659, 1771
Alw21I GWGCWC 3 cut(s) 292, 1270, 1602
Alw26I GTCTC 3 cut(s) 134, 381, 477
AlwI GGATC 2 cut(s) 257, 1403
AoxI GGCC 5 cut(s) 189, 389, 1205, 1477, 1555
ApeKI GCWGC 4 cut(s) 1306, 1391, 1394, 1445
ApoI RAATTY 3 cut(s) 720, 821, 831
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
AsuC2I CCSGG 2 cut(s) 27, 401
AsuHPI GGTGA 1 cut(s) 644
AvaII GGWCC 3 cut(s) 880, 890, 1612
BalI TGGCCA 2 cut(s) 391, 1207
BbsI GAAGAC 2 cut(s) 134, 948
Bbv12I GWGCWC 3 cut(s) 292, 1270, 1602
BbvI GCAGC 4 cut(s) 1318, 1403, 1406, 1432
BciT130I CCWGG 3 cut(s) 49, 501, 867
BciVI GTATCC 1 cut(s) 362
BcnI CCSGG 2 cut(s) 27, 401
BcoDI GTCTC 3 cut(s) 134, 381, 477
BfaI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
BfmI CTRYAG 2 cut(s) 936, 1566
BfuAI ACCTGC 1 cut(s) 846
BfuI GTATCC 1 cut(s) 362
BglI GCCNNNNNGGC 1 cut(s) 1632
BglII AGATCT 2 cut(s) 556, 973
BisI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
BlsI GCNGC 5 cut(s) 190, 1308, 1393, 1396, 1447
Bme1390I CCNGG 5 cut(s) 27, 49, 401, 501, 867
Bme18I GGWCC 3 cut(s) 880, 890, 1612
BmgT120I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
BmiI GGNNCC 3 cut(s) 192, 881, 1478
BmrFI CCNGG 5 cut(s) 27, 49, 401, 501, 867
BmsI GCATC 5 cut(s) 747, 791, 1571, 1626, 1660
BpiI GAAGAC 2 cut(s) 134, 948
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 5 cut(s) 31, 182, 309, 345, 888
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 678
BpuMI CCSGG 2 cut(s) 27, 401
BsaAI YACGTR 1 cut(s) 1597
BsaBI GATNNNNATC 1 cut(s) 1083
BsaJI CCNNGG 4 cut(s) 48, 266, 733, 812
BsaXI ACNNNNNCTCC 2 cut(s) 1249, 1279
Bsc4I CCNNNNNNNGG 5 cut(s) 365, 506, 753, 1334, 1609
Bse1I ACTGG 4 cut(s) 189, 326, 610, 1505
Bse3DI GCAATG 2 cut(s) 1020, 1136
Bse8I GATNNNNATC 1 cut(s) 1083
BseBI CCWGG 3 cut(s) 49, 501, 867
BseDI CCNNGG 4 cut(s) 48, 266, 733, 812
BseGI GGATG 7 cut(s) 929, 1021, 1194, 1617, 1675, 1701, 1712
BseJI GATNNNNATC 1 cut(s) 1083
BseLI CCNNNNNNNGG 5 cut(s) 365, 506, 753, 1334, 1609
BseMI GCAATG 2 cut(s) 1020, 1136
BseMII CTCAG 3 cut(s) 114, 461, 764
BseNI ACTGG 4 cut(s) 189, 326, 610, 1505
BseXI GCAGC 4 cut(s) 1318, 1403, 1406, 1432
BseYI CCCAGC 1 cut(s) 1327
BshFI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
BsiHKAI GWGCWC 3 cut(s) 292, 1270, 1602
BsiSI CCGG 2 cut(s) 26, 400
BslI CCNNNNNNNGG 5 cut(s) 365, 506, 753, 1334, 1609
BsmAI GTCTC 3 cut(s) 134, 381, 477
BsmI GAATGC 3 cut(s) 230, 350, 855
BsnI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
Bsp1286I GDGCHC 3 cut(s) 292, 1270, 1602
Bsp1407I TGTACA 1 cut(s) 1002
Bsp19I CCATGG 1 cut(s) 812
BspACI CCGC 5 cut(s) 87, 188, 913, 1213, 1615
BspANI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
BspCNI CTCAG 3 cut(s) 115, 462, 763
BspHI TCATGA 2 cut(s) 685, 1422
BspLI GGNNCC 3 cut(s) 192, 881, 1478
BspMAI CTGCAG 1 cut(s) 1570
BspMI ACCTGC 1 cut(s) 846
BspPI GGATC 2 cut(s) 257, 1403
BsrBI CCGCTC 1 cut(s) 1213
BsrDI GCAATG 2 cut(s) 1020, 1136
BsrGI TGTACA 1 cut(s) 1002
BsrI ACTGG 4 cut(s) 189, 326, 610, 1505
BssECI CCNNGG 4 cut(s) 48, 266, 733, 812
BssT1I CCWWGG 3 cut(s) 266, 733, 812
Bst2UI CCWGG 3 cut(s) 49, 501, 867
Bst4CI ACNGT 4 cut(s) 60, 661, 794, 1375
BstAPI GCANNNNNTGC 2 cut(s) 976, 1685
BstAUI TGTACA 1 cut(s) 1002
BstBAI YACGTR 1 cut(s) 1597
BstC8I GCNNGC 2 cut(s) 917, 1389
BstDEI CTNAG 4 cut(s) 123, 174, 470, 750
BstDSI CCRYGG 1 cut(s) 812
BstF5I GGATG 7 cut(s) 929, 1021, 1194, 1617, 1675, 1701, 1712
BstHHI GCGC 1 cut(s) 34
BstMAI GTCTC 3 cut(s) 134, 381, 477
BstMWI GCNNNNNNNGC 5 cut(s) 976, 1581, 1623, 1632, 1685
BstNI CCWGG 3 cut(s) 49, 501, 867
BstNSI RCATGY 1 cut(s) 1223
BstSCI CCNGG 5 cut(s) 25, 47, 399, 499, 865
BstSFI CTRYAG 2 cut(s) 936, 1566
BstV1I GCAGC 4 cut(s) 1318, 1403, 1406, 1432
BstV2I GAAGAC 2 cut(s) 134, 948
BstX2I RGATCY 3 cut(s) 262, 556, 973
BstXI CCANNNNNNTGG 3 cut(s) 273, 387, 435
BstYI RGATCY 3 cut(s) 262, 556, 973
BsuI GTATCC 1 cut(s) 362
BsuRI GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
BtgI CCRYGG 1 cut(s) 812
BtgZI GCGATG 1 cut(s) 836
BtsCI GGATG 7 cut(s) 929, 1021, 1194, 1617, 1675, 1701, 1712
BtsI GCAGTG 3 cut(s) 235, 316, 1454
BtsIMutI CAGTG 6 cut(s) 182, 235, 316, 603, 790, 1454
BveI ACCTGC 1 cut(s) 846
Cac8I GCNNGC 2 cut(s) 917, 1389
CciI TCATGA 2 cut(s) 685, 1422
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
Csp6I GTAC 4 cut(s) 68, 415, 1003, 1129
CviQI GTAC 4 cut(s) 68, 415, 1003, 1129
DdeI CTNAG 4 cut(s) 123, 174, 470, 750
EaeI YGGCCR 2 cut(s) 389, 1205
Eco130I CCWWGG 3 cut(s) 266, 733, 812
Eco47I GGWCC 3 cut(s) 880, 890, 1612
Eco57I CTGAAG 1 cut(s) 1292
EcoO109I RGGNCCY 2 cut(s) 880, 1477
EcoRI GAATTC 2 cut(s) 720, 821
EcoRII CCWGG 3 cut(s) 47, 499, 865
EcoT14I CCWWGG 3 cut(s) 266, 733, 812
ErhI CCWWGG 3 cut(s) 266, 733, 812
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 2 cut(s) 430, 538
Fnu4HI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
FokI GGATG 7 cut(s) 936, 1028, 1181, 1604, 1682, 1708, 1719
Fsp4HI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
FspBI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 1 cut(s) 33
GluI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
GsaI CCCAGC 1 cut(s) 1331
GsuI CTGGAG 5 cut(s) 31, 182, 309, 345, 888
HaeIII GGCC 5 cut(s) 191, 391, 1207, 1479, 1557
HapII CCGG 2 cut(s) 26, 400
HhaI GCGC 1 cut(s) 34
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HindIII AAGCTT 2 cut(s) 1509, 1769
HinfI GANTC 4 cut(s) 111, 179, 1118, 1652
HpaII CCGG 2 cut(s) 26, 400
HphI GGTGA 1 cut(s) 644
Hpy166II GTNNAC 3 cut(s) 893, 901, 1131
Hpy188I TCNGA 9 cut(s) 124, 462, 590, 753, 1045, 1072, 1243, 1272, 1352
Hpy8I GTNNAC 3 cut(s) 893, 901, 1131
HpyAV CCTTC 4 cut(s) 226, 313, 473, 1735
HpyCH4III ACNGT 4 cut(s) 60, 661, 794, 1375
HpyCH4IV ACGT 3 cut(s) 715, 1234, 1596
HpyF10VI GCNNNNNNNGC 5 cut(s) 976, 1581, 1623, 1632, 1685
HpyF3I CTNAG 4 cut(s) 123, 174, 470, 750
HpySE526I ACGT 3 cut(s) 715, 1234, 1596
HspAI GCGC 1 cut(s) 32
LmnI GCTCC 3 cut(s) 50, 1329, 1605
Lsp1109I GCAGC 4 cut(s) 1318, 1403, 1406, 1432
LweI GCATC 5 cut(s) 747, 791, 1571, 1626, 1660
MaeI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
MaeII ACGT 3 cut(s) 715, 1234, 1596
MaeIII GTNAC 1 cut(s) 180
MbiI CCGCTC 1 cut(s) 1213
MboII GAAGA 8 cut(s) 106, 139, 851, 953, 1306, 1428, 1532, 1730
MfeI CAATTG 1 cut(s) 1401
MflI RGATCY 3 cut(s) 262, 556, 973
MhlI GDGCHC 3 cut(s) 292, 1270, 1602
MlsI TGGCCA 2 cut(s) 391, 1207
MluCI AATT 7 cut(s) 463, 720, 821, 831, 998, 1401, 1644
MluNI TGGCCA 2 cut(s) 391, 1207
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 1315
Mox20I TGGCCA 2 cut(s) 391, 1207
MscI TGGCCA 2 cut(s) 391, 1207
MseI TTAA 3 cut(s) 441, 548, 656
MslI CAYNNNNRTG 3 cut(s) 1281, 1427, 1551
Msp20I TGGCCA 2 cut(s) 391, 1207
MspA1I CMGCKG 1 cut(s) 915
MspI CCGG 2 cut(s) 26, 400
MspR9I CCNGG 5 cut(s) 27, 49, 401, 501, 867
MunI CAATTG 1 cut(s) 1401
Mva1269I GAATGC 3 cut(s) 230, 350, 855
MvaI CCWGG 3 cut(s) 49, 501, 867
MwoI GCNNNNNNNGC 5 cut(s) 976, 1581, 1623, 1632, 1685
NciI CCSGG 2 cut(s) 27, 401
NcoI CCATGG 1 cut(s) 812
NdeI CATATG 2 cut(s) 430, 538
NlaIV GGNNCC 3 cut(s) 192, 881, 1478
NmuCI GTSAC 1 cut(s) 180
NsbI TGCGCA 1 cut(s) 33
NspI RCATGY 1 cut(s) 1223
PagI TCATGA 2 cut(s) 685, 1422
PcsI WCGNNNNNNNCGW 1 cut(s) 859
PctI GAATGC 3 cut(s) 230, 350, 855
PfeI GAWTC 3 cut(s) 111, 1118, 1652
PflMI CCANNNNNTGG 1 cut(s) 1334
PfoI TCCNGGA 1 cut(s) 865
PkrI GCNGC 5 cut(s) 190, 1308, 1393, 1396, 1447
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 1597
PpuMI RGGWCCY 1 cut(s) 880
Psp5II RGGWCCY 1 cut(s) 880
Psp6I CCWGG 3 cut(s) 47, 499, 865
PspFI CCCAGC 1 cut(s) 1327
PspGI CCWGG 3 cut(s) 47, 499, 865
PspN4I GGNNCC 3 cut(s) 192, 881, 1478
PspPI GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
PspPPI RGGWCCY 1 cut(s) 880
PstI CTGCAG 1 cut(s) 1570
PsuI RGATCY 3 cut(s) 262, 556, 973
RsaI GTAC 4 cut(s) 69, 416, 1004, 1130
RsaNI GTAC 4 cut(s) 68, 415, 1003, 1129
RseI CAYNNNNRTG 3 cut(s) 1281, 1427, 1551
SaqAI TTAA 3 cut(s) 441, 548, 656
SatI GCNGC 5 cut(s) 189, 1307, 1392, 1395, 1446
Sau96I GGNCC 5 cut(s) 190, 880, 890, 1477, 1612
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 5 cut(s) 27, 49, 401, 501, 867
SduI GDGCHC 3 cut(s) 292, 1270, 1602
SfaNI GCATC 5 cut(s) 747, 791, 1571, 1626, 1660
SfcI CTRYAG 2 cut(s) 936, 1566
SinI GGWCC 3 cut(s) 880, 890, 1612
SmiMI CAYNNNNRTG 3 cut(s) 1281, 1427, 1551
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
Sse9I AATT 7 cut(s) 463, 720, 821, 831, 998, 1401, 1644
SsiI CCGC 5 cut(s) 87, 188, 913, 1213, 1615
SspI AATATT 1 cut(s) 1752
SspMI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
StyD4I CCNGG 5 cut(s) 25, 47, 399, 499, 865
StyI CCWWGG 3 cut(s) 266, 733, 812
TaaI ACNGT 4 cut(s) 60, 661, 794, 1375
TaiI ACGT 3 cut(s) 718, 1237, 1599
TaqI TCGA 1 cut(s) 600
TasI AATT 7 cut(s) 463, 720, 821, 831, 998, 1401, 1644
TatI WGTACW 1 cut(s) 1002
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 3 cut(s) 111, 1118, 1652
Tru1I TTAA 3 cut(s) 441, 548, 656
Tru9I TTAA 3 cut(s) 441, 548, 656
TscAI CASTG 6 cut(s) 189, 235, 323, 610, 797, 1461
TseFI GTSAC 1 cut(s) 180
TseI GCWGC 4 cut(s) 1306, 1391, 1394, 1445
Tsp45I GTSAC 1 cut(s) 180
TspRI CASTG 6 cut(s) 189, 235, 323, 610, 797, 1461
Van91I CCANNNNNTGG 1 cut(s) 1334
VpaK11BI GGWCC 3 cut(s) 880, 890, 1612
XapI RAATTY 3 cut(s) 720, 821, 831
XbaI TCTAGA 2 cut(s) 797, 1796
XceI RCATGY 1 cut(s) 1223
XcmI CCANNNNNNNNNTGG 2 cut(s) 1122, 1663
XspI CTAG 6 cut(s) 350, 798, 842, 1263, 1604, 1797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.