RchiOBHm_Chr7g0231741
ERF Family

Ethylene-responsive transcription factor ERF022-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005624
Physical Location & Seq
Forward (+)
55470439 .. 55470916
478 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20769

Sequence Viewer

Length: 408 bp
ATGACCCAAGAAATTAATCAAACCCTCCGGGCAGATATGGAAGAAGTACACGAGCCTGCGCCCAAGTACAGAGGCGTACGCAAGCGAAAATGGGGTAAATGGGTGTCCGAAATCCGGGAACCGGGAAAGAAAACCCGGATATGGCTAGGAAGCTACGAGGCCCCCGAAATGGCGGCCGCGGCCTACGATGCGGCGTTGCACCTCAGAGGACCAGGGGTGTCGCTTAACTTTCCTGAAATGGTTGATAGTCTTCCGAGGCCGGCGAGTTCGAGTCCCTCGGACGTGCAGTTGGCGGCTCAAGAGGCGGCAGTGAGGTTTAGGAGACTGCCAATGGGGTCATTTTTTGACGGAGGTGGCTGGTGGCTCCAGTGGTGGCGCGGTTTCAGAGACGGTGGGACTGTCTCCTAG

Protein Analysis

135

Amino Acids

15.26

Weight (kDa)

9.45

Isoelectric Point (pI)

53.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 21 - 65 9e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 179, 378
AciI CCGC 7 cut(s) 173, 177, 179, 191, 293, 305, 378
AcoI YGGCCR 1 cut(s) 174
AfaI GTAC 3 cut(s) 48, 68, 78
AfiI CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 316, 381
AoxI GGCC 4 cut(s) 159, 174, 180, 257
AseI ATTAAT 1 cut(s) 15
AspLEI GCGC 2 cut(s) 61, 378
AspS9I GGNCC 2 cut(s) 160, 209
AsuC2I CCSGG 4 cut(s) 29, 116, 123, 136
AvaII GGWCC 1 cut(s) 209
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 242
BciT130I CCWGG 1 cut(s) 213
BcnI CCSGG 4 cut(s) 29, 116, 123, 136
BcoDI GTCTC 2 cut(s) 316, 381
BfaI CTAG 2 cut(s) 146, 406
BisI GCNGC 6 cut(s) 174, 177, 180, 192, 294, 306
BlsI GCNGC 6 cut(s) 175, 178, 181, 193, 295, 307
Bme1390I CCNGG 5 cut(s) 29, 116, 123, 136, 213
Bme18I GGWCC 1 cut(s) 209
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 160, 209
BmiI GGNNCC 3 cut(s) 120, 162, 365
BmrFI CCNGG 5 cut(s) 29, 116, 123, 136, 213
BmsI GCATC 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 282
BpuMI CCSGG 4 cut(s) 29, 116, 123, 136
BsaJI CCNNGG 4 cut(s) 177, 212, 254, 276
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
Bse118I RCCGGY 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 367
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 4 cut(s) 177, 212, 254, 276
BseLI CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
BseMII CTCAG 1 cut(s) 217
BseNI ACTGG 1 cut(s) 367
BseX3I CGGCCG 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 2 cut(s) 179, 378
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 4 cut(s) 161, 176, 182, 259
BsiEI CGRYCG 1 cut(s) 177
BsiSI CCGG 5 cut(s) 28, 115, 122, 136, 260
BsiWI CGTACG 1 cut(s) 76
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 4 cut(s) 114, 121, 141, 169
BsmAI GTCTC 2 cut(s) 316, 381
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 1 cut(s) 258
BsnI GGCC 4 cut(s) 161, 176, 182, 259
BspACI CCGC 7 cut(s) 173, 177, 179, 191, 293, 305, 378
BspANI GGCC 4 cut(s) 161, 176, 182, 259
BspCNI CTCAG 1 cut(s) 216
BspFNI CGCG 2 cut(s) 179, 378
BspLI GGNNCC 3 cut(s) 120, 162, 365
BsrFI RCCGGY 1 cut(s) 259
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 4 cut(s) 177, 212, 254, 276
BssSI CACGAG 1 cut(s) 50
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 2 cut(s) 392, 400
BstC8I GCNNGC 3 cut(s) 57, 83, 261
BstDEI CTNAG 1 cut(s) 203
BstDSI CCRYGG 1 cut(s) 177
BstFNI CGCG 2 cut(s) 179, 378
BstHHI GCGC 2 cut(s) 61, 378
BstMAI GTCTC 2 cut(s) 316, 381
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 3 cut(s) 179, 188, 302
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 5 cut(s) 27, 114, 121, 134, 211
BstUI CGCG 2 cut(s) 179, 378
BstV2I GAAGAC 1 cut(s) 242
BstZI CGGCCG 1 cut(s) 174
BsuRI GGCC 4 cut(s) 161, 176, 182, 259
BtgI CCRYGG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 283
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 315, 374
Cac8I GCNNGC 3 cut(s) 57, 83, 261
CciNI GCGGCCGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 61, 378
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 2 cut(s) 160, 209
Cfr42I CCGCGG 1 cut(s) 180
Csp6I GTAC 3 cut(s) 47, 67, 77
CviQI GTAC 3 cut(s) 47, 67, 77
DdeI CTNAG 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 174
EagI CGGCCG 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 209
Eco52I CGGCCG 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 381
FaiI YATR 2 cut(s) 38, 142
FaqI GGGAC 1 cut(s) 258
Fnu4HI GCNGC 6 cut(s) 174, 177, 180, 192, 294, 306
Fsp4HI GCNGC 6 cut(s) 174, 177, 180, 192, 294, 306
FspBI CTAG 2 cut(s) 146, 406
GlaI GCGC 2 cut(s) 60, 377
GluI GCNGC 6 cut(s) 174, 177, 180, 192, 294, 306
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 4 cut(s) 161, 176, 182, 259
HapII CCGG 5 cut(s) 28, 115, 122, 136, 260
HhaI GCGC 2 cut(s) 61, 378
Hin6I GCGC 2 cut(s) 59, 376
HinP1I GCGC 2 cut(s) 59, 376
HinfI GANTC 1 cut(s) 271
HpaII CCGG 5 cut(s) 28, 115, 122, 136, 260
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 5 cut(s) 109, 206, 255, 280, 386
Hpy188III TCNNGA 2 cut(s) 233, 299
Hpy8I GTNNAC 1 cut(s) 49
HpyCH4III ACNGT 2 cut(s) 392, 400
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 2 cut(s) 199, 286
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 188, 302
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 1 cut(s) 282
HspAI GCGC 2 cut(s) 59, 376
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
KspI CCGCGG 1 cut(s) 180
LmnI GCTCC 1 cut(s) 369
LweI GCATC 1 cut(s) 178
MaeI CTAG 2 cut(s) 146, 406
MaeII ACGT 1 cut(s) 282
MboII GAAGA 2 cut(s) 53, 242
MluCI AATT 1 cut(s) 12
MlyI GAGTC 1 cut(s) 280
MroNI GCCGGC 1 cut(s) 259
MseI TTAA 2 cut(s) 15, 225
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 5 cut(s) 28, 115, 122, 136, 260
MspR9I CCNGG 5 cut(s) 29, 116, 123, 136, 213
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 2 cut(s) 179, 378
MwoI GCNNNNNNNGC 3 cut(s) 179, 188, 302
NaeI GCCGGC 1 cut(s) 261
NciI CCSGG 4 cut(s) 29, 116, 123, 136
NgoMIV GCCGGC 1 cut(s) 259
NlaIV GGNNCC 3 cut(s) 120, 162, 365
NotI GCGGCCGC 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 261
Pfl23II CGTACG 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 114
PkrI GCNGC 6 cut(s) 175, 178, 181, 193, 295, 307
PleI GAGTC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 279
PshBI ATTAAT 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspLI CGTACG 1 cut(s) 76
PspN4I GGNNCC 3 cut(s) 120, 162, 365
PspPI GGNCC 2 cut(s) 160, 209
RsaI GTAC 3 cut(s) 48, 68, 78
RsaNI GTAC 3 cut(s) 47, 67, 77
SacII CCGCGG 1 cut(s) 180
SaqAI TTAA 2 cut(s) 15, 225
SatI GCNGC 6 cut(s) 174, 177, 180, 192, 294, 306
Sau96I GGNCC 2 cut(s) 160, 209
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 5 cut(s) 29, 116, 123, 136, 213
SetI ASST 5 cut(s) 155, 204, 285, 317, 355
SfaNI GCATC 1 cut(s) 178
Sfr303I CCGCGG 1 cut(s) 180
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 1 cut(s) 12
SsiI CCGC 7 cut(s) 173, 177, 179, 191, 293, 305, 378
SspMI CTAG 2 cut(s) 146, 406
StyD4I CCNGG 5 cut(s) 27, 114, 121, 134, 211
TaaI ACNGT 2 cut(s) 392, 400
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 269
TasI AATT 1 cut(s) 12
TatI WGTACW 2 cut(s) 46, 66
TauI GCSGC 6 cut(s) 176, 179, 182, 194, 296, 308
Tru1I TTAA 2 cut(s) 15, 225
Tru9I TTAA 2 cut(s) 15, 225
TscAI CASTG 2 cut(s) 315, 374
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 2 cut(s) 315, 374
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 15
XspI CTAG 2 cut(s) 146, 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.