FvH4_6g05280

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003953
Physical Location & Seq
Reverse (-)
2970728 .. 2972829
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05280.t1

Sequence Viewer

Length: 1494 bp
ATGGCTTCCCAAGCTCAGGTTTCTGTAGACAGATTCAGTGAACTTCCACTAGAAATGGCTCATCACATTCTGAGTTTCCTTTCCATAGCTGATGTCATTCGAGTGAGCAGCTTATCGAAAACATGCAAAGGTCTACATCTATCGAATCCTATTTTGACTATGGATTCATTTCCGTTCACCCTCACATCTGATAAGAGGTTGGAGTTGTTCAACTCGTTGGATCGCTTCCTGGTTCAACGCGGGAACAACAAGGTAGAATCCTTTATCTTTGAAATGGAGTCTGTATACCAGCATGGGGAAACCGAAATAGAAAGTCACTGTAGTTTTGAGAAATCACGAATTCTGAGTTGGATTCAAAATGTAGTAGATCATTGCAACGTGGAAGAGCTTCATGTTGTCTTTGAATCTTTTATGTCCAATCCAGAAGATTATAGTCTTGATGTTAAGATAGATTTTCCTGCCTGTATCTTTCTTTGCCAGTCATTGAGTTACTTAAACCTGCAAATGGACTGTGCAATACTTAAAGCTCCCTCCTTCTCTACTTGTCCCCCCTCTAATCTCGTGCACTTGATCCTGACACATGTAACAATAGAAGACGGTGAAGGGTTTTGCAAATGGGTCTCTTACTGTTGTAGGTTCATTAAGGAATTGAGGCTGGCACTAGTTTCTGGTTTGGATAACATCACCGTTCAAAGTTCGTCTTTGGAGTACTTTTGGGTGCACTCAGATGTTCTTTCGAATCTTGTAATATCTGGTGATAGAATTCAAGAAGTATATATAGACTGGGAGTATGATGTAACCACCAAGCAGAGCAATAAAAGTTTGAACATTTCGGCACCAAATCTGAAGTGTCTGACATGGATTGGGAATATGTTGGATCATCAGCATCTTGGGCAAGTGAATTGTTTGGAAAAACTTGTTATTTCTTTGACTTGTTATGATGTAAATGATTTGGATAGCGTTTGTGAGCAGTTCCTTTGCAGTATAAGAAGTGTTAAAGTTCTTTTTCTAGACGCAGTGACTGCCAAGACTTTGTTCAGGAAGGGATTCAGACCTGCTCCATTGAGCAATGTTTGCGACTTGCTTATGTTTATTCGGTGCAGTATTGATGACAACCTTGTGCCAGCCATGACCTCTCTATTTAGGGCTGTGCCCAATTTGATTTATTTGAATGTGTCATCTTCTTCTCAATTTCCTATTCCACTTGCTCTTCTAAACCTTCTTGGTTGCGATGATAATATATCTGGATCTGATGGGGGATGTTGGGAACTCCAGGGGCTTGATTTTGTTTCTAAGGTGGAGGAGGTAATCATAGGGCTTAATTCAGGTTGCAATGGATTTGAATTAGCAAGGTACATGCTTGAGCATGCTCGTAATTTGAAAAAACTGTCTGTTATCACTACACCTGATGATCAATCAAGTTCAATACCGGATATATGGAGAAGCAACCCTTCTTTCAATTCTAGAGTTGTCTTTGTGGAAGAGGATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

498

Amino Acids

56.07

Weight (kDa)

4.88

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 117 - 302 2.4e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 507, 1063
AccB1I GGYRCC 1 cut(s) 835
AccI GTMKAC 3 cut(s) 27, 133, 285
AccII CGCG 1 cut(s) 240
AciI CCGC 1 cut(s) 240
AclWI GGATC 4 cut(s) 228, 565, 885, 1255
AcsI RAATTY 2 cut(s) 339, 762
AcuI CTGAAG 1 cut(s) 866
AfaI GTAC 2 cut(s) 710, 1355
AfiI CCNNNNNNNGG 3 cut(s) 16, 295, 505
AflIII ACRYGT 1 cut(s) 580
AhlI ACTAGT 1 cut(s) 661
AjnI CCWGG 2 cut(s) 228, 1272
AjuI GAANNNNNNNTTGG 4 cut(s) 331, 363, 1019, 1051
AluBI AGCT 5 cut(s) 14, 89, 111, 388, 527
AluI AGCT 5 cut(s) 14, 89, 111, 388, 527
Alw21I GWGCWC 2 cut(s) 567, 723
Alw26I GTCTC 1 cut(s) 625
Alw44I GTGCAC 2 cut(s) 563, 719
AlwI GGATC 4 cut(s) 228, 565, 885, 1255
AlwNI CAGNNNCTG 1 cut(s) 1022
ApaLI GTGCAC 2 cut(s) 563, 719
ApeKI GCWGC 1 cut(s) 108
ApoI RAATTY 2 cut(s) 339, 762
Asp700I GAANNNNTTC 2 cut(s) 387, 1046
AsuHPI GGTGA 4 cut(s) 169, 611, 676, 767
AsuII TTCGAA 1 cut(s) 737
BaeGI GKGCMC 3 cut(s) 567, 723, 1155
BanI GGYRCC 1 cut(s) 835
BauI CACGAG 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 600
Bbv12I GWGCWC 2 cut(s) 567, 723
BbvI GCAGC 1 cut(s) 120
BccI CCATC 1 cut(s) 1247
BciT130I CCWGG 2 cut(s) 230, 1274
BclI TGATCA 1 cut(s) 1411
BcoDI GTCTC 1 cut(s) 625
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 5 cut(s) 50, 662, 1010, 1464, 1492
BfmI CTRYAG 2 cut(s) 24, 319
BfuAI ACCTGC 2 cut(s) 507, 1063
BisI GCNGC 1 cut(s) 109
BlsI GCNGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 710
Bme1390I CCNGG 2 cut(s) 230, 1274
BmiI GGNNCC 1 cut(s) 837
BmrFI CCNGG 2 cut(s) 230, 1274
BmrI ACTGGG 1 cut(s) 793
BmsI GCATC 1 cut(s) 895
BmuI ACTGGG 1 cut(s) 793
BpiI GAAGAC 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 1256
Bpu10I CCTNAGC 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 737
BpuEI CTTGAG 1 cut(s) 1382
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 1 cut(s) 1273
BsaWI WCCGGW 1 cut(s) 1429
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 295, 505
Bse1I ACTGG 2 cut(s) 478, 788
Bse3DI GCAATG 3 cut(s) 370, 1075, 1339
BseBI CCWGG 2 cut(s) 230, 1274
BseDI CCNNGG 1 cut(s) 1273
BseGI GGATG 1 cut(s) 1265
BseLI CCNNNNNNNGG 3 cut(s) 16, 295, 505
BseMI GCAATG 3 cut(s) 370, 1075, 1339
BseMII CTCAG 4 cut(s) 29, 62, 335, 738
BseNI ACTGG 2 cut(s) 478, 788
BseRI GAGGAG 1 cut(s) 1316
BseSI GKGCMC 3 cut(s) 567, 723, 1155
BseXI GCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 1120
Bsh1236I CGCG 1 cut(s) 240
BshNI GGYRCC 1 cut(s) 835
BsiHKAI GWGCWC 2 cut(s) 567, 723
BsiSI CCGG 1 cut(s) 1430
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 3 cut(s) 16, 295, 505
BsmAI GTCTC 1 cut(s) 625
BsmFI GGGAC 1 cut(s) 531
Bso31I GGTCTC 1 cut(s) 625
Bsp119I TTCGAA 1 cut(s) 737
Bsp1286I GDGCHC 3 cut(s) 567, 723, 1155
Bsp143I GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
BspACI CCGC 1 cut(s) 240
BspCNI CTCAG 4 cut(s) 28, 63, 336, 737
BspFNI CGCG 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 837
BspMI ACCTGC 2 cut(s) 507, 1063
BspPI GGATC 4 cut(s) 228, 565, 885, 1255
BspQI GCTCTTC 2 cut(s) 378, 1215
BspT104I TTCGAA 1 cut(s) 737
BspT107I GGYRCC 1 cut(s) 835
BspTNI GGTCTC 1 cut(s) 625
BsrDI GCAATG 3 cut(s) 370, 1075, 1339
BsrI ACTGG 2 cut(s) 478, 788
BssECI CCNNGG 1 cut(s) 1273
BssMI GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
BssNAI GTATAC 1 cut(s) 286
BssSI CACGAG 1 cut(s) 560
Bst1107I GTATAC 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 560
Bst2UI CCWGG 2 cut(s) 230, 1274
Bst4CI ACNGT 6 cut(s) 320, 512, 599, 629, 688, 1389
Bst6I CTCTTC 3 cut(s) 378, 1215, 1476
BstAPI GCANNNNNTGC 2 cut(s) 1022, 1074
BstBI TTCGAA 1 cut(s) 737
BstC8I GCNNGC 3 cut(s) 657, 1125, 1368
BstDEI CTNAG 5 cut(s) 15, 71, 344, 724, 1293
BstF5I GGATG 1 cut(s) 1265
BstFNI CGCG 1 cut(s) 240
BstKTI GATC 6 cut(s) 223, 370, 573, 880, 1250, 1414
BstMAI GTCTC 1 cut(s) 625
BstMBI GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
BstMWI GCNNNNNNNGC 4 cut(s) 11, 892, 1022, 1074
BstNI CCWGG 2 cut(s) 230, 1274
BstNSI RCATGY 4 cut(s) 126, 584, 1360, 1370
BstSCI CCNGG 2 cut(s) 228, 1272
BstSFI CTRYAG 2 cut(s) 24, 319
BstSLI GKGCMC 3 cut(s) 567, 723, 1155
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 1 cut(s) 1247
BstYI RGATCY 1 cut(s) 1247
BstZ17I GTATAC 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 1245
BtsCI GGATG 1 cut(s) 1265
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 3 cut(s) 43, 316, 1023
BveI ACCTGC 2 cut(s) 507, 1063
Cac8I GCNNGC 3 cut(s) 657, 1125, 1368
CaiI CAGNNNCTG 1 cut(s) 1022
CseI GACGC 1 cut(s) 1022
Csp6I GTAC 2 cut(s) 709, 1354
CviAII CATG 8 cut(s) 123, 293, 392, 581, 858, 1129, 1357, 1367
CviQI GTAC 2 cut(s) 709, 1354
DdeI CTNAG 5 cut(s) 15, 71, 344, 724, 1293
DpnI GATC 6 cut(s) 222, 369, 572, 879, 1249, 1413
DpnII GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
Eam1104I CTCTTC 3 cut(s) 378, 1215, 1476
EarI CTCTTC 3 cut(s) 378, 1215, 1476
Eco31I GGTCTC 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 866
EcoRI GAATTC 2 cut(s) 339, 762
EcoRII CCWGG 2 cut(s) 228, 1272
FaeI CATG 8 cut(s) 126, 296, 395, 584, 861, 1132, 1360, 1370
FalI AAGNNNNNCTT 4 cut(s) 685, 717, 1435, 1467
FaqI GGGAC 1 cut(s) 531
FatI CATG 8 cut(s) 122, 292, 391, 580, 857, 1128, 1356, 1366
FauI CCCGC 1 cut(s) 233
FbaI TGATCA 1 cut(s) 1411
FblI GTMKAC 3 cut(s) 27, 133, 285
Fnu4HI GCNGC 1 cut(s) 109
FokI GGATG 1 cut(s) 1272
Fsp4HI GCNGC 1 cut(s) 109
FspBI CTAG 5 cut(s) 50, 662, 1010, 1464, 1492
GluI GCNGC 1 cut(s) 109
GsuI CTGGAG 1 cut(s) 1256
HapII CCGG 1 cut(s) 1430
HgaI GACGC 1 cut(s) 1022
Hin1II CATG 8 cut(s) 126, 296, 395, 584, 861, 1132, 1360, 1370
HinfI GANTC 9 cut(s) 33, 145, 164, 257, 278, 352, 404, 739, 1047
HpaII CCGG 1 cut(s) 1430
HphI GGTGA 4 cut(s) 169, 611, 676, 767
Hpy166II GTNNAC 7 cut(s) 28, 41, 134, 177, 286, 565, 721
Hpy188I TCNGA 8 cut(s) 72, 190, 345, 727, 846, 855, 1052, 1252
Hpy188III TCNNGA 9 cut(s) 336, 422, 437, 574, 767, 1010, 1039, 1245, 1464
Hpy8I GTNNAC 7 cut(s) 28, 41, 134, 177, 286, 565, 721
HpyAV CCTTC 5 cut(s) 544, 596, 1036, 1229, 1461
HpyCH4III ACNGT 6 cut(s) 320, 512, 599, 629, 688, 1389
HpyCH4IV ACGT 1 cut(s) 378
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 892, 1022, 1074
HpyF3I CTNAG 5 cut(s) 15, 71, 344, 724, 1293
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 8 cut(s) 126, 296, 395, 584, 861, 1132, 1360, 1370
Ksp22I TGATCA 1 cut(s) 1411
Kzo9I GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
LguI GCTCTTC 2 cut(s) 378, 1215
LmnI GCTCC 2 cut(s) 532, 1063
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 1 cut(s) 895
MaeI CTAG 5 cut(s) 50, 662, 1010, 1464, 1492
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 5 cut(s) 314, 488, 583, 796, 1018
MalI GATC 6 cut(s) 222, 369, 572, 879, 1249, 1413
MboI GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
MboII GAAGA 7 cut(s) 395, 437, 605, 1173, 1176, 1202, 1493
MflI RGATCY 1 cut(s) 1247
MhlI GDGCHC 3 cut(s) 567, 723, 1155
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 4 cut(s) 180, 198, 329, 855
MnlI CCTC 9 cut(s) 189, 191, 541, 562, 645, 1144, 1294, 1297, 1477
MroXI GAANNNNTTC 2 cut(s) 387, 1046
MseI TTAA 6 cut(s) 444, 494, 522, 642, 996, 1320
MslI CAYNNNNRTG 2 cut(s) 101, 726
MspI CCGG 1 cut(s) 1430
MspR9I CCNGG 2 cut(s) 230, 1274
MvaI CCWGG 2 cut(s) 230, 1274
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 4 cut(s) 11, 892, 1022, 1074
NdeII GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
NlaIII CATG 8 cut(s) 126, 296, 395, 584, 861, 1132, 1360, 1370
NlaIV GGNNCC 1 cut(s) 837
NmuCI GTSAC 2 cut(s) 314, 1018
NspI RCATGY 4 cut(s) 126, 584, 1360, 1370
NspV TTCGAA 1 cut(s) 737
PaeI GCATGC 1 cut(s) 1370
PciI ACATGT 1 cut(s) 580
PciSI GCTCTTC 2 cut(s) 378, 1215
PdmI GAANNNNTTC 2 cut(s) 387, 1046
PfeI GAWTC 8 cut(s) 33, 145, 164, 257, 352, 404, 739, 1047
PkrI GCNGC 1 cut(s) 110
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PscI ACATGT 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 228, 1272
PspGI CCWGG 2 cut(s) 228, 1272
PspN4I GGNNCC 1 cut(s) 837
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 1 cut(s) 1247
RsaI GTAC 2 cut(s) 710, 1355
RsaNI GTAC 2 cut(s) 709, 1354
RseI CAYNNNNRTG 2 cut(s) 101, 726
SapI GCTCTTC 2 cut(s) 378, 1215
SaqAI TTAA 6 cut(s) 444, 494, 522, 642, 996, 1320
SatI GCNGC 1 cut(s) 109
Sau3AI GATC 6 cut(s) 220, 367, 570, 877, 1247, 1411
ScaI AGTACT 1 cut(s) 710
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 230, 1274
SduI GDGCHC 3 cut(s) 567, 723, 1155
SfaNI GCATC 1 cut(s) 895
SfcI CTRYAG 2 cut(s) 24, 319
SfuI TTCGAA 1 cut(s) 737
SmiMI CAYNNNNRTG 2 cut(s) 101, 726
SmlI CTYRAG 1 cut(s) 1361
SmoI CTYRAG 1 cut(s) 1361
SpeI ACTAGT 1 cut(s) 661
SphI GCATGC 1 cut(s) 1370
SsiI CCGC 1 cut(s) 240
SspMI CTAG 5 cut(s) 50, 662, 1010, 1464, 1492
StyD4I CCNGG 2 cut(s) 228, 1272
TaaI ACNGT 6 cut(s) 320, 512, 599, 629, 688, 1389
TaiI ACGT 1 cut(s) 381
TaqI TCGA 4 cut(s) 100, 116, 143, 737
TatI WGTACW 1 cut(s) 708
TfiI GAWTC 8 cut(s) 33, 145, 164, 257, 352, 404, 739, 1047
Tru1I TTAA 6 cut(s) 444, 494, 522, 642, 996, 1320
Tru9I TTAA 6 cut(s) 444, 494, 522, 642, 996, 1320
TscAI CASTG 3 cut(s) 43, 323, 1023
TseFI GTSAC 2 cut(s) 314, 1018
TseI GCWGC 1 cut(s) 108
Tsp45I GTSAC 2 cut(s) 314, 1018
TspDTI ATGAA 3 cut(s) 156, 380, 628
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 3 cut(s) 43, 323, 1023
VneI GTGCAC 2 cut(s) 563, 719
XapI RAATTY 2 cut(s) 339, 762
XbaI TCTAGA 2 cut(s) 1009, 1463
XceI RCATGY 4 cut(s) 126, 584, 1360, 1370
XmiI GTMKAC 3 cut(s) 27, 133, 285
XmnI GAANNNNTTC 2 cut(s) 387, 1046
XspI CTAG 5 cut(s) 50, 662, 1010, 1464, 1492
ZrmI AGTACT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.