FvH4_6g09451
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002297
Physical Location & Seq
Reverse (-)
5628078 .. 5639609
11532 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09451.t1

Sequence Viewer

Length: 2541 bp
ATGTTGAGGTTGAAATTTTGGGGTTGTCTCATTGCTCTGGCTTGTTCTTGGTTCTTCCTAGTTGCAGTGGCACAAGTTACAAATCCTTCTGAAGTTGATGCATTGAGGGCAGTGAAGGGTAGTTTGATTGATCCCATGAAGCATTTGAGGAATTGGGACAAAGGGGATCCATGTATAGCGAATTGGACCGGAGTTTTGTGTTTCGGTGATGTTAGGACTGATGGATACTTGCATGTCCGGCAGCTGCAACTGCTGAGTATGAATCTCTCAGGAAGCTTAGCAGCACCAGAGCTTGGCAAACTATCTCAACTTCTGATATTAGATTTCATGTGGAATGACTTAAGTGGGACTATACCGAAGGAGATAGGAAACATTACGTCCTTGGAACTCTTGCTCTTGAACGGAAACAGACTATCAGGTTCCTTGCCTGATGAGCTTGGTTATCTTGAAAATCTAAATAGACTACAAGTTGACCAGAACCATCTTTCTGGTCCGGTACCAAAATCATTTGTTAACTTGCGCCGCTTAAAACACCTCCACTTGAATAACAACTCCTTCAGTGGCCAAATTCCATCTGAGATTTCTGGAATACCCACTCTTCTTCATGTTCAACTCGATAACAACAACTTCATGGGAACTGAGATTCCAGCTACCTATGGAAACCTTTCCAGATTGGCAAAGTTAAGTCTCAGAAACTGCAGTTTGCAGGGAACAATTCCTGATCTTAGCCAGATACCTAACCTCAGTTATCTAGATCTAAGCTGGAATCAGCTTGCTGGACCAATAGCATCACATAGACTTTCTGACAATATGACAACAATTGATCTGTCAGAAAACCATCTTAACGGATCTATTCCTGAAAGTTTCTCCAATCTTCCTCATCTTCAGAAATTGTCGCTCGAGAATAATTTGTTGAATGGTTCTGTCCCTGCTGTATGGAGGAACATATCTTTTAGTTCAAGAGCTAGACTTTCATTTGATCTCCGGAATAATTCACTCTCTAACATATTAGGAGAGCTGAATCCTCCAGCAAATGTCACCTTGAGGCTTGAAGGAAATCCTATTTGCAAGAATGCTAGCATAGAAAATATAATCCCGTTCTGTCAATCTGAAGTGAGAGATGACATCCCTGAAGATTCAATAAATTCAACTCAACAGATGAACTGCCCTATTCAAGCATGTCCAGTGGATGATTTCTATGAATATGTTCCATCTTCCCCGGTGCCATGCTTTTGTGCATCACCTATTATAATTGAATATCGTCTCAAAAGCCCAAGCTTTTCTGATTTTCCTCCATATATACAGCAGTTTGAAAAATATTTAACTACTTCTCTCAAATTGGAGATTTATCAGTTATCAATAAGTTCCTTTGTGTGGGAAATGGGGCCTCGCTTACGGATGCAGTTGAAGTTCTTCCCTGTGTATGGTGCTTCACACTCGTATACATTTAACAAAAGTGAGGTTCTGCGGATTAGAGGCATCTTTACAGAATGGCTATTTCCTCCAAATGATTTCTTTGGACCTTATGAGCTACACAAGTTCAATCTGCTGGGACCTTATTCAAATATGATTGTTAAGAGACAAAGGATGGTCATCAGTAAGGGTGTATTAGCAGCTGTTATAATAGGGGCCATTGCTTCGGTTGTCGGTGTATCTGCAACAGTCATGCTATTGTTCACAAGATGCCGCAGAAACCGATACCCACCACCTTCAAGGAGACACTCATCGTCGAAGATTTCTATGAGAATTGAAGGTGTAAAGTCTTTCACTTTCAAAGAAATGACGCTAGCCACCAGGAACTTTGATAGTTCAACTCAACTTGGACGAGGAGGTTATGGGAAAGTTTACAAAGGTGTCTTATCTGATAACACAATTGTAGCCATAAAACGTGCAGAAGAAGGATCCCTACAGGGTGAAAAAGAATTCTTGACTGAGATACAATTGTTATCGAGGCTACACCACCGAAACTTAGTCTCGTTGGTAGGATACTGTGAAGAAGAAGAGGAGCAGGTGTTGGTTTATGAGTTCATGCCTAATGGTACCTTACGTGACTGGCTTTCTGATAAAATCAAGGGAAGCCTGGACTTCAGTATGAGGTTACGTATTGCATTGGGTTCAGCCAAAGGAATTCTTTATCTCCATACCGAGGCGAATCCTCCTATATTTCACCGAGATATTAAAGCCACCAACATACTTTTGGACTCCAACTATACTGCTAAGGTGGCTGATTTTGGACTCTCACGACTTGCTCCTGTCCAGGATGATGAAGGGACTGGCCCTGGATACGTATCTACAGTTGTGAGAGGAACACCGGGCTACCTGGATCCAGAGTATTTCTTGACCAGTCAGTTAACAGACAAAAGCGATGTCTATAGCCTTGGAATTGTGTTCCTGGAGCTCTTGACTGGTGTGCTGCCAATATCACATGGCAAAAACATTGTTCGGGAGGTGAATATGGCTCACCAGAGTGACCAGACAGGCTTGATGTTCTCCATTATAGGGGAGAACGGGAGATACCGGCCAGAATGGCTGGGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

847

Amino Acids

94.65

Weight (kDa)

6.82

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 67 1.6e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 77 - 160 3.4e-06 Leucine-rich repeat region
LRR_8 PF13855 151 - 210 1.2e-06 Leucine rich repeat
LRR_14 PF23598 166 - 319 3.7e-07 Leucine-rich repeat region
LRR_8 PF13855 270 - 305 3.1e-06 Leucine rich repeat
Pkinase PF00069 603 - 820 2.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 604 - 810 3.9e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1250, 1622
AarI CACCTGC 1 cut(s) 1999
Acc36I ACCTGC 1 cut(s) 1999
Acc65I GGTACC 2 cut(s) 496, 2039
AccB1I GGYRCC 3 cut(s) 496, 1222, 2039
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 1 cut(s) 1442
AccIII TCCGGA 1 cut(s) 984
AciI CCGC 3 cut(s) 523, 1468, 1687
AclWI GGATC 8 cut(s) 125, 161, 174, 856, 1896, 1909, 2318, 2331
AcoI YGGCCR 2 cut(s) 562, 2519
AcsI RAATTY 5 cut(s) 14, 567, 1144, 1922, 2127
AcuI CTGAAG 6 cut(s) 111, 541, 869, 1131, 1152, 2071
AfaI GTAC 2 cut(s) 498, 2041
AfiI CCNNNNNNNGG 5 cut(s) 293, 1374, 1424, 2146, 2499
AflII CTTAAG 1 cut(s) 340
AjnI CCWGG 6 cut(s) 1793, 2079, 2256, 2278, 2319, 2391
AleI CACNNNNGTG 1 cut(s) 2466
AloI GAACNNNNNNTCC 2 cut(s) 628, 660
Alw21I GWGCWC 1 cut(s) 2400
Alw26I GTCTC 6 cut(s) 32, 692, 1268, 1573, 1711, 1978
AlwI GGATC 8 cut(s) 125, 161, 174, 856, 1896, 1909, 2318, 2331
AlwNI CAGNNNCTG 1 cut(s) 696
Ama87I CYCGRG 1 cut(s) 899
Aor13HI TCCGGA 1 cut(s) 984
AoxI GGCC 5 cut(s) 562, 1385, 1629, 2275, 2519
ApeKI GCWGC 5 cut(s) 241, 244, 281, 1613, 2413
ApoI RAATTY 5 cut(s) 14, 567, 1144, 1922, 2127
ArsI GACNNNNNNTTYG 4 cut(s) 178, 210, 2352, 2384
Asp700I GAANNNNTTC 3 cut(s) 664, 1206, 1412
Asp718I GGTACC 2 cut(s) 496, 2039
AspLEI GCGC 1 cut(s) 522
AspS9I GGNCC 8 cut(s) 186, 491, 779, 1385, 1520, 1553, 1629, 2276
AsuC2I CCSGG 2 cut(s) 1220, 2313
AsuHPI GGTGA 7 cut(s) 218, 1030, 1233, 1925, 2159, 2453, 2461
AsuNHI GCTAGC 2 cut(s) 1076, 1786
AvaI CYCGRG 1 cut(s) 899
AvaII GGWCC 5 cut(s) 186, 491, 779, 1520, 1553
BaeI ACNNNNGTAYC 2 cut(s) 1928, 1961
BalI TGGCCA 1 cut(s) 564
BamHI GGATCC 3 cut(s) 166, 1901, 2323
BanI GGYRCC 3 cut(s) 496, 1222, 2039
BanII GRGCYC 1 cut(s) 2400
BarI GAAGNNNNNNTAC 2 cut(s) 1890, 1922
Bbv12I GWGCWC 1 cut(s) 2400
BbvI GCAGC 5 cut(s) 231, 253, 293, 1625, 2400
BccI CCATC 6 cut(s) 215, 489, 580, 846, 1219, 1582
BciT130I CCWGG 6 cut(s) 1795, 2081, 2258, 2280, 2321, 2393
BciVI GTATCC 3 cut(s) 218, 1979, 2276
BcnI CCSGG 2 cut(s) 1220, 2313
BcoDI GTCTC 6 cut(s) 32, 692, 1268, 1573, 1711, 1978
BfaI CTAG 5 cut(s) 59, 752, 966, 1077, 1787
BfmI CTRYAG 4 cut(s) 697, 1907, 2292, 2371
BfrI CTTAAG 1 cut(s) 340
BfuAI ACCTGC 1 cut(s) 1999
BfuI GTATCC 3 cut(s) 218, 1979, 2276
BglI GCCNNNNNGGC 1 cut(s) 2527
BglII AGATCT 1 cut(s) 754
BisI GCNGC 7 cut(s) 242, 245, 282, 523, 1614, 1687, 2414
BlpI GCTNAGC 1 cut(s) 277
BlsI GCNGC 7 cut(s) 243, 246, 283, 524, 1615, 1688, 2415
Bme1390I CCNGG 8 cut(s) 1220, 1795, 2081, 2258, 2280, 2313, 2321, 2393
Bme18I GGWCC 5 cut(s) 186, 491, 779, 1520, 1553
BmeT110I CYCGRG 1 cut(s) 899
BmgT120I GGNCC 8 cut(s) 186, 491, 779, 1385, 1520, 1553, 1629, 2276
BmrFI CCNGG 8 cut(s) 1220, 1795, 2081, 2258, 2280, 2313, 2321, 2393
BmsI GCATC 6 cut(s) 88, 797, 1247, 1389, 1488, 1673
BmtI GCTAGC 2 cut(s) 1080, 1790
BpmI CTGGAG 2 cut(s) 1011, 2414
Bpu10I CCTNAGC 1 cut(s) 2217
Bpu1102I GCTNAGC 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 1063
BpuMI CCSGG 2 cut(s) 1220, 2313
BsaAI YACGTR 3 cut(s) 2048, 2102, 2287
BsaBI GATNNNNATC 2 cut(s) 1592, 2287
BsaJI CCNNGG 5 cut(s) 381, 1218, 2145, 2278, 2377
BsaWI WCCGGW 3 cut(s) 188, 493, 984
BsaXI ACNNNNNCTCC 2 cut(s) 1997, 2027
Bsc4I CCNNNNNNNGG 5 cut(s) 293, 1374, 1424, 2146, 2499
Bse118I RCCGGY 1 cut(s) 2517
Bse1I ACTGG 5 cut(s) 1184, 2057, 2278, 2343, 2410
Bse3DI GCAATG 2 cut(s) 30, 1632
Bse8I GATNNNNATC 2 cut(s) 1592, 2287
BseAI TCCGGA 1 cut(s) 984
BseBI CCWGG 6 cut(s) 1795, 2081, 2258, 2280, 2321, 2393
BseDI CCNNGG 5 cut(s) 381, 1218, 2145, 2278, 2377
BseGI GGATG 5 cut(s) 1125, 1195, 1404, 1593, 2266
BseJI GATNNNNATC 2 cut(s) 1592, 2287
BseLI CCNNNNNNNGG 5 cut(s) 293, 1374, 1424, 2146, 2499
BseMI GCAATG 2 cut(s) 30, 1632
BseMII CTCAG 7 cut(s) 245, 282, 567, 630, 703, 757, 1923
BseNI ACTGG 5 cut(s) 1184, 2057, 2278, 2343, 2410
BseRI GAGGAG 2 cut(s) 1842, 2018
BseXI GCAGC 5 cut(s) 231, 253, 293, 1625, 2400
BseYI CCCAGC 2 cut(s) 1549, 2530
BsgI GTGCAG 1 cut(s) 1911
BshFI GGCC 5 cut(s) 564, 1387, 1631, 2277, 2521
BshNI GGYRCC 3 cut(s) 496, 1222, 2039
BsiHKAI GWGCWC 1 cut(s) 2400
BsiHKCI CYCGRG 1 cut(s) 899
BsiSI CCGG 7 cut(s) 189, 238, 494, 985, 1220, 2312, 2518
BslFI GGGAC 5 cut(s) 170, 361, 911, 1566, 2284
BslI CCNNNNNNNGG 5 cut(s) 293, 1374, 1424, 2146, 2499
BsmAI GTCTC 6 cut(s) 32, 692, 1268, 1573, 1711, 1978
BsmBI CGTCTC 1 cut(s) 1268
BsmFI GGGAC 5 cut(s) 170, 361, 911, 1566, 2284
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 5 cut(s) 564, 1387, 1631, 2277, 2521
BsoBI CYCGRG 1 cut(s) 899
Bsp1286I GDGCHC 1 cut(s) 2400
Bsp13I TCCGGA 1 cut(s) 984
Bsp143I GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
Bsp1720I GCTNAGC 1 cut(s) 277
BspACI CCGC 3 cut(s) 523, 1468, 1687
BspANI GGCC 5 cut(s) 564, 1387, 1631, 2277, 2521
BspCNI CTCAG 7 cut(s) 246, 281, 568, 631, 702, 756, 1924
BspEI TCCGGA 1 cut(s) 984
BspMAI CTGCAG 1 cut(s) 701
BspMI ACCTGC 1 cut(s) 1999
BspOI GCTAGC 2 cut(s) 1080, 1790
BspPI GGATC 8 cut(s) 125, 161, 174, 856, 1896, 1909, 2318, 2331
BspT107I GGYRCC 3 cut(s) 496, 1222, 2039
BspTI CTTAAG 1 cut(s) 340
BsrDI GCAATG 2 cut(s) 30, 1632
BsrFI RCCGGY 1 cut(s) 2517
BsrI ACTGG 5 cut(s) 1184, 2057, 2278, 2343, 2410
BssAI RCCGGY 1 cut(s) 2517
BssECI CCNNGG 5 cut(s) 381, 1218, 2145, 2278, 2377
BssMI GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
BssNAI GTATAC 1 cut(s) 1443
BssT1I CCWWGG 2 cut(s) 381, 2377
Bst1107I GTATAC 1 cut(s) 1443
Bst2UI CCWGG 6 cut(s) 1795, 2081, 2258, 2280, 2321, 2393
Bst4CI ACNGT 3 cut(s) 1663, 1991, 2296
Bst6I CTCTTC 2 cut(s) 603, 1995
BstAFI CTTAAG 1 cut(s) 340
BstBAI YACGTR 3 cut(s) 2048, 2102, 2287
BstC8I GCNNGC 3 cut(s) 774, 1078, 1788
BstF5I GGATG 5 cut(s) 1125, 1195, 1404, 1593, 2266
BstHHI GCGC 1 cut(s) 522
BstKTI GATC 9 cut(s) 133, 169, 724, 757, 826, 851, 982, 1904, 2326
BstMAI GTCTC 6 cut(s) 32, 692, 1268, 1573, 1711, 1978
BstMBI GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
BstMWI GCNNNNNNNGC 6 cut(s) 107, 238, 250, 433, 2222, 2527
BstNI CCWGG 6 cut(s) 1795, 2081, 2258, 2280, 2321, 2393
BstNSI RCATGY 2 cut(s) 236, 1182
BstSCI CCNGG 8 cut(s) 1218, 1793, 2079, 2256, 2278, 2311, 2319, 2391
BstSFI CTRYAG 4 cut(s) 697, 1907, 2292, 2371
BstSNI TACGTA 2 cut(s) 2102, 2287
BstV1I GCAGC 5 cut(s) 231, 253, 293, 1625, 2400
BstX2I RGATCY 5 cut(s) 166, 754, 848, 1901, 2323
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 5 cut(s) 166, 754, 848, 1901, 2323
BstZ17I GTATAC 1 cut(s) 1443
BsuI GTATCC 3 cut(s) 218, 1979, 2276
BsuRI GGCC 5 cut(s) 564, 1387, 1631, 2277, 2521
BtgZI GCGATG 1 cut(s) 2379
BtsCI GGATG 5 cut(s) 1125, 1195, 1404, 1593, 2266
BtsI GCAGTG 2 cut(s) 72, 117
BtsIMutI CAGTG 4 cut(s) 72, 117, 565, 1191
BveI ACCTGC 1 cut(s) 1999
Cac8I GCNNGC 3 cut(s) 774, 1078, 1788
CaiI CAGNNNCTG 1 cut(s) 696
CfoI GCGC 1 cut(s) 522
Cfr10I RCCGGY 1 cut(s) 2517
Cfr13I GGNCC 8 cut(s) 186, 491, 779, 1385, 1520, 1553, 1629, 2276
CseI GACGC 1 cut(s) 1792
Csp6I GTAC 2 cut(s) 497, 2040
CviQI GTAC 2 cut(s) 497, 2040
DpnI GATC 9 cut(s) 132, 168, 723, 756, 825, 850, 981, 1903, 2325
DpnII GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
EaeI YGGCCR 2 cut(s) 562, 2519
Eam1104I CTCTTC 2 cut(s) 603, 1995
EarI CTCTTC 2 cut(s) 603, 1995
Ecl136II GAGCTC 1 cut(s) 2398
Eco105I TACGTA 2 cut(s) 2102, 2287
Eco130I CCWWGG 2 cut(s) 381, 2377
Eco24I GRGCYC 1 cut(s) 2400
Eco47I GGWCC 5 cut(s) 186, 491, 779, 1520, 1553
Eco53kI GAGCTC 1 cut(s) 2398
Eco57I CTGAAG 6 cut(s) 111, 541, 869, 1131, 1152, 2071
Eco88I CYCGRG 1 cut(s) 899
EcoICRI GAGCTC 1 cut(s) 2398
EcoO109I RGGNCCY 2 cut(s) 1385, 1553
EcoRI GAATTC 2 cut(s) 1922, 2127
EcoRII CCWGG 6 cut(s) 1793, 2079, 2256, 2278, 2319, 2391
EcoT14I CCWWGG 2 cut(s) 381, 2377
EcoT22I ATGCAT 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 2400
ErhI CCWWGG 2 cut(s) 381, 2377
Esp3I CGTCTC 1 cut(s) 1268
FalI AAGNNNNNCTT 2 cut(s) 2115, 2147
FaqI GGGAC 5 cut(s) 170, 361, 911, 1566, 2284
FblI GTMKAC 1 cut(s) 1442
Fnu4HI GCNGC 7 cut(s) 242, 245, 282, 523, 1614, 1687, 2414
FokI GGATG 5 cut(s) 1112, 1202, 1411, 1600, 2273
FriOI GRGCYC 1 cut(s) 2400
Fsp4HI GCNGC 7 cut(s) 242, 245, 282, 523, 1614, 1687, 2414
FspBI CTAG 5 cut(s) 59, 752, 966, 1077, 1787
GlaI GCGC 1 cut(s) 521
GluI GCNGC 7 cut(s) 242, 245, 282, 523, 1614, 1687, 2414
GsaI CCCAGC 2 cut(s) 1553, 2534
GsuI CTGGAG 2 cut(s) 1011, 2414
HaeIII GGCC 5 cut(s) 564, 1387, 1631, 2277, 2521
HapII CCGG 7 cut(s) 189, 238, 494, 985, 1220, 2312, 2518
HgaI GACGC 1 cut(s) 1792
HhaI GCGC 1 cut(s) 522
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HincII GTYRAC 3 cut(s) 472, 514, 2352
HindII GTYRAC 3 cut(s) 472, 514, 2352
HindIII AAGCTT 2 cut(s) 274, 1276
HinfI GANTC 8 cut(s) 262, 643, 766, 1021, 1136, 2152, 2201, 2235
HpaI GTTAAC 2 cut(s) 514, 2352
HpaII CCGG 7 cut(s) 189, 238, 494, 985, 1220, 2312, 2518
HphI GGTGA 7 cut(s) 218, 1030, 1233, 1925, 2159, 2453, 2461
Hpy166II GTNNAC 6 cut(s) 472, 514, 1443, 1677, 1846, 2352
Hpy8I GTNNAC 6 cut(s) 472, 514, 1443, 1677, 1846, 2352
Hpy99I CGWCG 1 cut(s) 1732
HpyAV CCTTC 9 cut(s) 96, 109, 352, 565, 1046, 1719, 1745, 1892, 2261
HpyCH4III ACNGT 3 cut(s) 1663, 1991, 2296
HpyCH4IV ACGT 5 cut(s) 377, 1888, 2047, 2101, 2286
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 238, 250, 433, 2222, 2527
HpySE526I ACGT 5 cut(s) 377, 1888, 2047, 2101, 2286
HspAI GCGC 1 cut(s) 520
Kpn2I TCCGGA 1 cut(s) 984
KpnI GGTACC 2 cut(s) 500, 2043
KspAI GTTAAC 2 cut(s) 514, 2352
Kzo9I GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
LmnI GCTCC 3 cut(s) 2005, 2254, 2395
Lsp1109I GCAGC 5 cut(s) 231, 253, 293, 1625, 2400
LweI GCATC 6 cut(s) 88, 797, 1247, 1389, 1488, 1673
MaeI CTAG 5 cut(s) 59, 752, 966, 1077, 1787
MaeII ACGT 5 cut(s) 377, 1888, 2047, 2101, 2286
MaeIII GTNAC 5 cut(s) 76, 1036, 2048, 2097, 2468
MalI GATC 9 cut(s) 132, 168, 723, 756, 825, 850, 981, 1903, 2325
MboI GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
MfeI CAATTG 3 cut(s) 819, 1872, 1940
MflI RGATCY 5 cut(s) 166, 754, 848, 1901, 2323
MhlI GDGCHC 1 cut(s) 2400
MlsI TGGCCA 1 cut(s) 564
MluNI TGGCCA 1 cut(s) 564
MlyI GAGTC 2 cut(s) 2195, 2229
MmeI TCCRAC 1 cut(s) 2229
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 103
MroI TCCGGA 1 cut(s) 984
MroXI GAANNNNTTC 3 cut(s) 664, 1206, 1412
MscI TGGCCA 1 cut(s) 564
MslI CAYNNNNRTG 1 cut(s) 2466
Msp20I TGGCCA 1 cut(s) 564
MspA1I CMGCKG 2 cut(s) 244, 1616
MspCI CTTAAG 1 cut(s) 340
MspI CCGG 7 cut(s) 189, 238, 494, 985, 1220, 2312, 2518
MspR9I CCNGG 8 cut(s) 1220, 1795, 2081, 2258, 2280, 2313, 2321, 2393
MunI CAATTG 3 cut(s) 819, 1872, 1940
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 6 cut(s) 1795, 2081, 2258, 2280, 2321, 2393
MwoI GCNNNNNNNGC 6 cut(s) 107, 238, 250, 433, 2222, 2527
NciI CCSGG 2 cut(s) 1220, 2313
NdeII GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
NheI GCTAGC 2 cut(s) 1076, 1786
NmuCI GTSAC 3 cut(s) 1036, 2048, 2468
NsiI ATGCAT 1 cut(s) 103
NspI RCATGY 2 cut(s) 236, 1182
OliI CACNNNNGTG 1 cut(s) 2466
PaeR7I CTCGAG 1 cut(s) 899
PaqCI CACCTGC 1 cut(s) 1999
PctI GAATGC 1 cut(s) 1078
PdmI GAANNNNTTC 3 cut(s) 664, 1206, 1412
PfeI GAWTC 6 cut(s) 262, 643, 766, 1021, 1136, 2152
PflMI CCANNNNNTGG 1 cut(s) 293
PfoI TCCNGGA 2 cut(s) 2256, 2391
PkrI GCNGC 7 cut(s) 243, 246, 283, 524, 1615, 1688, 2415
PleI GAGTC 2 cut(s) 2195, 2229
PpsI GAGTC 2 cut(s) 2195, 2229
Ppu21I YACGTR 3 cut(s) 2048, 2102, 2287
PpuMI RGGWCCY 1 cut(s) 1553
PsiI TTATAA 2 cut(s) 1250, 1622
Psp124BI GAGCTC 1 cut(s) 2400
Psp5II RGGWCCY 1 cut(s) 1553
Psp6I CCWGG 6 cut(s) 1793, 2079, 2256, 2278, 2319, 2391
PspFI CCCAGC 2 cut(s) 1549, 2530
PspGI CCWGG 6 cut(s) 1793, 2079, 2256, 2278, 2319, 2391
PspPI GGNCC 8 cut(s) 186, 491, 779, 1385, 1520, 1553, 1629, 2276
PspPPI RGGWCCY 1 cut(s) 1553
PsrI GAACNNNNNNTAC 2 cut(s) 2498, 2530
PstI CTGCAG 1 cut(s) 701
PstNI CAGNNNCTG 1 cut(s) 696
PsuI RGATCY 5 cut(s) 166, 754, 848, 1901, 2323
PvuII CAGCTG 2 cut(s) 244, 1616
RsaI GTAC 2 cut(s) 498, 2041
RsaNI GTAC 2 cut(s) 497, 2040
RseI CAYNNNNRTG 1 cut(s) 2466
SacI GAGCTC 1 cut(s) 2400
SatI GCNGC 7 cut(s) 242, 245, 282, 523, 1614, 1687, 2414
Sau3AI GATC 9 cut(s) 130, 166, 721, 754, 823, 848, 979, 1901, 2323
Sau96I GGNCC 8 cut(s) 186, 491, 779, 1385, 1520, 1553, 1629, 2276
SchI GAGTC 2 cut(s) 2195, 2229
ScrFI CCNGG 8 cut(s) 1220, 1795, 2081, 2258, 2280, 2313, 2321, 2393
SduI GDGCHC 1 cut(s) 2400
SfaNI GCATC 6 cut(s) 88, 797, 1247, 1389, 1488, 1673
SfcI CTRYAG 4 cut(s) 697, 1907, 2292, 2371
Sfr274I CTCGAG 1 cut(s) 899
SinI GGWCC 5 cut(s) 186, 491, 779, 1520, 1553
SlaI CTCGAG 1 cut(s) 899
SmiMI CAYNNNNRTG 1 cut(s) 2466
SmlI CTYRAG 3 cut(s) 340, 899, 1042
SmoI CTYRAG 3 cut(s) 340, 899, 1042
SnaBI TACGTA 2 cut(s) 2102, 2287
SsiI CCGC 3 cut(s) 523, 1468, 1687
SspI AATATT 1 cut(s) 1319
SspMI CTAG 5 cut(s) 59, 752, 966, 1077, 1787
SstI GAGCTC 1 cut(s) 2400
StyD4I CCNGG 8 cut(s) 1218, 1793, 2079, 2256, 2278, 2311, 2319, 2391
StyI CCWWGG 2 cut(s) 381, 2377
TaaI ACNGT 3 cut(s) 1663, 1991, 2296
TaiI ACGT 5 cut(s) 380, 1891, 2050, 2104, 2289
TaqI TCGA 4 cut(s) 615, 900, 1730, 1949
TauI GCSGC 2 cut(s) 525, 1689
TfiI GAWTC 6 cut(s) 262, 643, 766, 1021, 1136, 2152
TscAI CASTG 4 cut(s) 72, 117, 565, 1191
TseFI GTSAC 3 cut(s) 1036, 2048, 2468
TseI GCWGC 5 cut(s) 241, 244, 281, 1613, 2413
Tsp45I GTSAC 3 cut(s) 1036, 2048, 2468
TspGWI ACGGA 3 cut(s) 417, 861, 1411
TspRI CASTG 4 cut(s) 72, 117, 565, 1191
Van91I CCANNNNNTGG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 340
VpaK11BI GGWCC 5 cut(s) 186, 491, 779, 1520, 1553
XapI RAATTY 5 cut(s) 14, 567, 1144, 1922, 2127
XbaI TCTAGA 1 cut(s) 751
XceI RCATGY 2 cut(s) 236, 1182
XcmI CCANNNNNNNNNTGG 1 cut(s) 2194
XhoI CTCGAG 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 1442
XmnI GAANNNNTTC 3 cut(s) 664, 1206, 1412
XspI CTAG 5 cut(s) 59, 752, 966, 1077, 1787
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.