RchiOBHm_Chr3g0460021
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002297
Physical Location & Seq
Reverse (-)
8259982 .. 8269434
9453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42656

Sequence Viewer

Length: 2565 bp
ATGTTGAGACTGAGATTTTGGGGTTGCCTCATTGGTCTGGCTTGTTCTTGCTTCTTCCTAGTTGCGGTTGCACAAGTTACTAATCCTTTTGAAGTGGATGCATTGAGAGCAGTGAAGGGTAGTCTGATTGATTCCGGGAATCATTTGGGGAATTGGGACAAAGGGGATCCATGTAAAGCGAATTGGACCGGAGTTTTGTGCTCCGGTGACGTTGGGACTGATGGATACTTGCATGTCCAGGAGCTGTATGTGCAACTGCTAAGTAAGAATCTCTCAGGAACGTTAGCGCCTGAGCTTGGCAAACTATCTCAACTTCTGATATTAGATTTCATGTGGAATAACTTAAGTGGGACTATACCAAAGGAGATAGGAAACATTACGTCCTTGAAACTCTTGCTCTTGAATGGAAACAAACTATCGGGTTCCTTGCCTGATGAGCTCGGTTCTCTTGAAAATCTACGTAGACTACAAGTTGACGAGAACCATCTTTCAGGTTCAATACCAAAATCATTTCTTAACTTGCGCGGCTTAAAACACCTCCATATGAACAACAACTCCTTCAGCGGCCAAATTCCATCTGAGATTTCTGAAATACCCGCTCTTCTTCATGTGCTCTTGGATAATAATAACTTATCTGGATATCTTCCACCAGACTTCTCCAACATACCAGAGTTGCGCATACTTCAACTCGATAACAACAACTTCACGGGAACTGAGATTCCAGCTACTTATGGAACCCTTTCCGGATTGGTAAAGTTAAGTCTCAGGAACTGCAGTTTGCAGGGAGCAATTCCTGATCTTAGCCGGATACGTAACCTCAGTTATCTAGATCTAAGCTGGAATCATCTTGCTGGACCGATACCATCACATGGGCTTTCTGACAATATGACAACAATTGTTCTGTCAGATAATCATCTTAGCGGATCTATTCCTGAAGGTTTCTCTCATTTTCCTCGTCTTCAGAAATTGTCGCTAAAAAATAATCAGTTGAACGGCTCTGTCCCTGCTATATGGAGGAACATATCTTTTAGTACAAGAGCTAGACTTTCATTTGATCTCCGGAACAATTCACTCTCTAACATATTGGGAGAGCTGAATCCTCCAGCAAATGTCACCTTGTGGCTTGAAGGAAATCCTATTTGCAAGAATGCTAGCATAGAAAATATAAGCCCGTTCTGTCAATCTGAAGCGAGCGATGGGATCCCCAAAGATTCAATAAATTCAACTCATAAGATGACCTGCCTTATTCAGGAATGTCCAGTGGATGATTTCTATGAATATGTTACATCTTCCCCTGTGCCATGCTTTTGTGCATCACCTATTATAGTTGAATATCGCCTGAAAAGCCCGAGCTTTTCTTACTTTGGTCCATATAAACAGCAGTTTGAAGAGTATATAACTAGGTCTCTAAAATTGGAGATTTACCAGTTATATATTAGTTCCTTTGTCTGGGAAGAAGGGCCTCGTTTACGGATGCAGTTGAAGTTCTTCCCTGTGTATGGTGCTTCACACTCGCATGCATTAAATGCAAGTGAGGTTCTGCGAATTAGAGGCATCTTTACATCATGGGAATTTTCTCAGAATCAATTCTTTGGACCATATGAGCTACTCGACTTCAAACTGCTGGGACCTTATTCAAATATGATTGTTAAGAGACAAAAGATGGGCCTCAGTAAGGGTGTATTAGCAGCTGTTATAATAGGGGCCATTGCTTCTGTTGTCAGTATAACTGCAACAGTCATGCTTCTGTTCACAAGATGCACCAGAAACCGATACACACCACCTTCAAGGAGACACTCGTCGTGGAAGATTTCTATGAGAATTGAGGGTGTAAAGGCGTTCACTTTCAAAGAAATGACACTAGCCACCAGGAACTTTGATAGTGCATCTCAACTTGGACAAGGAGGCTATGGGAAAGTTTATAAAGGTATCTTATCTGATACCACAGCTGTAGCCATAAAGCGTGCAGAAGCAGGATCCCTACAGGGTGAAAGAGAATTCTTGACCGAGATACAATTATTATCAAGGCTACACCATCGAAACTTAGTCTCTTTGGTAGGATACTGTGAAGAAGAAGGGGAGCAGATGTTGGTTTATGAGTTCATGCCTAATGGTACCTTACGTGACTGGCTTTCTGATAAAACCAAGGGAAGCCTGGACTTCAGTATGAGATTACGTATTGCATTGGGTTCAGCCAAAGGAATTCTGTATCTCCATACCGAGGCAAATCCTCCTATATTCCACCGAGATATTAAAGCCACAAATATACTTTTGGACTCCAACTATGTCGCTAAGGTGGCTGATTTTGGACTCTCACGACTAGCACCTGTCCAGAATGATGAAGGGACTGGCCCTGCATACGTATCTACAGTCGTGAAAGGAACACCGGGGTACCTGGATCCAGAGTATTTCTTGACCAGGAAGTTAACAGACAAAAGCGATGTCTATAGCCTTGGAATTGTGTTTCTGGAGCTCTTGACTGGTGTGCTGCCAATATCACATGGCAAAAACATTGTTCGCGAGGTACCTATCATAACATTAAAACAGAAATTTTGTTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

854

Amino Acids

94.99

Weight (kDa)

8.21

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 68 4.5e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 152 - 211 7.1e-06 Leucine rich repeat
LRR_14 PF23598 172 - 336 8.8e-06 Leucine-rich repeat region
LRR_8 PF13855 223 - 283 1e-05 Leucine rich repeat
Pkinase PF00069 628 - 837 6.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 630 - 833 9.7e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1697, 1923
Acc16I TGCGCA 1 cut(s) 677
Acc36I ACCTGC 1 cut(s) 1247
Acc65I GGTACC 3 cut(s) 2114, 2391, 2524
AccB1I GGYRCC 3 cut(s) 2114, 2391, 2524
AccB7I CCANNNNNTGG 1 cut(s) 869
AccBSI CCGCTC 1 cut(s) 599
AccI GTMKAC 1 cut(s) 463
AccII CGCG 2 cut(s) 525, 2520
AccIII TCCGGA 2 cut(s) 743, 1059
AciI CCGC 5 cut(s) 65, 525, 564, 597, 921
AclI AACGTT 1 cut(s) 281
AclWI GGATC 9 cut(s) 161, 174, 931, 1195, 1208, 1971, 1984, 2393, 2406
AcoI YGGCCR 1 cut(s) 565
AcsI RAATTY 6 cut(s) 570, 1219, 1571, 1997, 2202, 2549
AcuI CTGAAG 5 cut(s) 544, 944, 954, 1206, 2146
AdeI CACNNNGTG 1 cut(s) 1119
AfaI GTAC 4 cut(s) 1033, 2116, 2393, 2526
AfiI CCNNNNNNNGG 8 cut(s) 64, 296, 869, 1249, 1449, 1499, 1675, 2221
AflII CTTAAG 1 cut(s) 343
AjnI CCWGG 5 cut(s) 237, 1868, 2154, 2394, 2417
AloI GAACNNNNNNTCC 4 cut(s) 539, 571, 703, 735
Alw21I GWGCWC 4 cut(s) 203, 441, 615, 2475
Alw26I GTCTC 5 cut(s) 767, 1410, 1648, 1786, 2053
AlwI GGATC 9 cut(s) 161, 174, 931, 1195, 1208, 1971, 1984, 2393, 2406
AlwNI CAGNNNCTG 2 cut(s) 244, 771
Ama87I CYCGRG 1 cut(s) 1348
Aor13HI TCCGGA 2 cut(s) 743, 1059
AoxI GGCC 5 cut(s) 565, 1460, 1666, 1704, 2350
ApeKI GCWGC 2 cut(s) 1688, 2488
ApoI RAATTY 6 cut(s) 570, 1219, 1571, 1997, 2202, 2549
ArsI GACNNNNNNTTYG 5 cut(s) 32, 178, 210, 2427, 2459
Asp700I GAANNNNTTC 3 cut(s) 739, 1487, 1586
Asp718I GGTACC 3 cut(s) 2114, 2391, 2524
AspLEI GCGC 3 cut(s) 289, 525, 678
AspS9I GGNCC 9 cut(s) 186, 854, 1367, 1460, 1595, 1628, 1666, 1704, 2351
AsuC2I CCSGG 2 cut(s) 136, 2388
AsuHPI GGTGA 4 cut(s) 218, 1105, 1308, 2000
AsuNHI GCTAGC 1 cut(s) 1151
AvaI CYCGRG 1 cut(s) 1348
AvaII GGWCC 5 cut(s) 186, 854, 1367, 1595, 1628
BaeI ACNNNNGTAYC 2 cut(s) 2375, 2408
BamHI GGATCC 4 cut(s) 166, 1200, 1976, 2398
BanI GGYRCC 3 cut(s) 2114, 2391, 2524
BanII GRGCYC 2 cut(s) 441, 2475
BbsI GAAGAC 1 cut(s) 950
Bbv12I GWGCWC 4 cut(s) 203, 441, 615, 2475
BbvI GCAGC 2 cut(s) 1700, 2475
BccI CCATC 7 cut(s) 215, 492, 583, 871, 1190, 1657, 2043
BceAI ACGGC 1 cut(s) 1009
BciT130I CCWGG 5 cut(s) 239, 1870, 2156, 2396, 2419
BciVI GTATCC 3 cut(s) 218, 801, 2054
BcnI CCSGG 2 cut(s) 136, 2388
BcoDI GTCTC 5 cut(s) 767, 1410, 1648, 1786, 2053
BfaI CTAG 7 cut(s) 59, 827, 1041, 1152, 1401, 1862, 2321
BfmI CTRYAG 5 cut(s) 772, 1950, 1982, 2367, 2446
BfoI RGCGCY 1 cut(s) 290
BfrI CTTAAG 1 cut(s) 343
BfuAI ACCTGC 1 cut(s) 1247
BfuI GTATCC 3 cut(s) 218, 801, 2054
BglII AGATCT 1 cut(s) 829
BisI GCNGC 4 cut(s) 526, 565, 1689, 2489
BlsI GCNGC 4 cut(s) 527, 566, 1690, 2490
Bme1390I CCNGG 7 cut(s) 136, 239, 1870, 2156, 2388, 2396, 2419
Bme18I GGWCC 5 cut(s) 186, 854, 1367, 1595, 1628
BmeT110I CYCGRG 1 cut(s) 1348
BmgT120I GGNCC 9 cut(s) 186, 854, 1367, 1460, 1595, 1628, 1666, 1704, 2351
BmrFI CCNGG 7 cut(s) 136, 239, 1870, 2156, 2388, 2396, 2419
BmsI GCATC 6 cut(s) 88, 1322, 1464, 1563, 1748, 1895
BmtI GCTAGC 1 cut(s) 1155
BoxI GACNNNNGTC 1 cut(s) 1798
BpiI GAAGAC 1 cut(s) 950
BpmI CTGGAG 2 cut(s) 1086, 2489
Bpu10I CCTNAGC 2 cut(s) 291, 2292
BpuMI CCSGG 2 cut(s) 136, 2388
BsaAI YACGTR 5 cut(s) 461, 812, 2123, 2177, 2362
BsaBI GATNNNNATC 1 cut(s) 912
BsaI GGTCTC 1 cut(s) 1410
BsaJI CCNNGG 4 cut(s) 2145, 2220, 2387, 2452
BsaWI WCCGGW 4 cut(s) 188, 203, 743, 1059
BsaXI ACNNNNNCTCC 4 cut(s) 539, 569, 2072, 2102
Bsc4I CCNNNNNNNGG 8 cut(s) 64, 296, 869, 1249, 1449, 1499, 1675, 2221
Bse1I ACTGG 5 cut(s) 1259, 1426, 2132, 2353, 2485
Bse3DI GCAATG 1 cut(s) 1707
Bse8I GATNNNNATC 1 cut(s) 912
BseAI TCCGGA 2 cut(s) 743, 1059
BseBI CCWGG 5 cut(s) 239, 1870, 2156, 2396, 2419
BseDI CCNNGG 4 cut(s) 2145, 2220, 2387, 2452
BseGI GGATG 3 cut(s) 103, 1270, 1479
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 8 cut(s) 64, 296, 869, 1249, 1449, 1499, 1675, 2221
BseMI GCAATG 1 cut(s) 1707
BseMII CTCAG 8 cut(s) 282, 288, 570, 705, 778, 832, 1592, 1684
BseNI ACTGG 5 cut(s) 1259, 1426, 2132, 2353, 2485
BseXI GCAGC 2 cut(s) 1700, 2475
BseYI CCCAGC 1 cut(s) 1624
BsgI GTGCAG 1 cut(s) 1986
Bsh1236I CGCG 2 cut(s) 525, 2520
BshFI GGCC 5 cut(s) 567, 1462, 1668, 1706, 2352
BshNI GGYRCC 3 cut(s) 2114, 2391, 2524
BsiHKAI GWGCWC 4 cut(s) 203, 441, 615, 2475
BsiHKCI CYCGRG 1 cut(s) 1348
BsiSI CCGG 7 cut(s) 135, 189, 204, 744, 805, 1060, 2387
BslFI GGGAC 6 cut(s) 170, 229, 364, 986, 1641, 2359
BslI CCNNNNNNNGG 8 cut(s) 64, 296, 869, 1249, 1449, 1499, 1675, 2221
BsmAI GTCTC 5 cut(s) 767, 1410, 1648, 1786, 2053
BsmFI GGGAC 6 cut(s) 170, 229, 364, 986, 1641, 2359
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 5 cut(s) 567, 1462, 1668, 1706, 2352
Bso31I GGTCTC 1 cut(s) 1410
BsoBI CYCGRG 1 cut(s) 1348
Bsp1286I GDGCHC 4 cut(s) 203, 441, 615, 2475
Bsp13I TCCGGA 2 cut(s) 743, 1059
Bsp143I GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
Bsp68I TCGCGA 1 cut(s) 2520
BspACI CCGC 5 cut(s) 65, 525, 564, 597, 921
BspANI GGCC 5 cut(s) 567, 1462, 1668, 1706, 2352
BspCNI CTCAG 8 cut(s) 283, 287, 571, 706, 777, 831, 1591, 1683
BspEI TCCGGA 2 cut(s) 743, 1059
BspFNI CGCG 2 cut(s) 525, 2520
BspMAI CTGCAG 1 cut(s) 776
BspMI ACCTGC 1 cut(s) 1247
BspOI GCTAGC 1 cut(s) 1155
BspPI GGATC 9 cut(s) 161, 174, 931, 1195, 1208, 1971, 1984, 2393, 2406
BspQI GCTCTTC 1 cut(s) 606
BspT107I GGYRCC 3 cut(s) 2114, 2391, 2524
BspTI CTTAAG 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 1410
BsrBI CCGCTC 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 1707
BsrI ACTGG 5 cut(s) 1259, 1426, 2132, 2353, 2485
BssECI CCNNGG 4 cut(s) 2145, 2220, 2387, 2452
BssMI GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
BssT1I CCWWGG 2 cut(s) 2145, 2452
Bst2UI CCWGG 5 cut(s) 239, 1870, 2156, 2396, 2419
Bst4CI ACNGT 3 cut(s) 1738, 2066, 2371
Bst6I CTCTTC 2 cut(s) 606, 1383
BstAFI CTTAAG 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 1526
BstBAI YACGTR 5 cut(s) 461, 812, 2123, 2177, 2362
BstC8I GCNNGC 4 cut(s) 1153, 1192, 1518, 1965
BstENI CCTNNNNNAGG 2 cut(s) 1247, 1673
BstF5I GGATG 3 cut(s) 103, 1270, 1479
BstFNI CGCG 2 cut(s) 525, 2520
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 3 cut(s) 289, 525, 678
BstKTI GATC 8 cut(s) 169, 799, 832, 926, 1057, 1203, 1979, 2401
BstMAI GTCTC 5 cut(s) 767, 1410, 1648, 1786, 2053
BstMBI GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
BstMWI GCNNNNNNNGC 6 cut(s) 107, 250, 436, 1344, 1526, 2297
BstNI CCWGG 5 cut(s) 239, 1870, 2156, 2396, 2419
BstNSI RCATGY 2 cut(s) 236, 1520
BstPAI GACNNNNGTC 1 cut(s) 1798
BstSCI CCNGG 7 cut(s) 134, 237, 1868, 2154, 2386, 2394, 2417
BstSFI CTRYAG 5 cut(s) 772, 1950, 1982, 2367, 2446
BstSNI TACGTA 4 cut(s) 461, 812, 2177, 2362
BstUI CGCG 2 cut(s) 525, 2520
BstV1I GCAGC 2 cut(s) 1700, 2475
BstV2I GAAGAC 1 cut(s) 950
BstX2I RGATCY 6 cut(s) 166, 829, 923, 1200, 1976, 2398
BstYI RGATCY 6 cut(s) 166, 829, 923, 1200, 1976, 2398
BsuI GTATCC 3 cut(s) 218, 801, 2054
BsuRI GGCC 5 cut(s) 567, 1462, 1668, 1706, 2352
BtgZI GCGATG 2 cut(s) 1209, 2454
BtsCI GGATG 3 cut(s) 103, 1270, 1479
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 117, 1266
BtuMI TCGCGA 1 cut(s) 2520
BveI ACCTGC 1 cut(s) 1247
Cac8I GCNNGC 4 cut(s) 1153, 1192, 1518, 1965
CaiI CAGNNNCTG 2 cut(s) 244, 771
CfoI GCGC 3 cut(s) 289, 525, 678
Cfr13I GGNCC 9 cut(s) 186, 854, 1367, 1460, 1595, 1628, 1666, 1704, 2351
Csp6I GTAC 4 cut(s) 1032, 2115, 2392, 2525
CviQI GTAC 4 cut(s) 1032, 2115, 2392, 2525
DpnI GATC 8 cut(s) 168, 798, 831, 925, 1056, 1202, 1978, 2400
DpnII GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
DraIII CACNNNGTG 1 cut(s) 1119
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 2 cut(s) 606, 1383
EarI CTCTTC 2 cut(s) 606, 1383
Ecl136II GAGCTC 2 cut(s) 439, 2473
Eco105I TACGTA 4 cut(s) 461, 812, 2177, 2362
Eco130I CCWWGG 2 cut(s) 2145, 2452
Eco24I GRGCYC 2 cut(s) 441, 2475
Eco31I GGTCTC 1 cut(s) 1410
Eco32I GATATC 1 cut(s) 641
Eco47I GGWCC 5 cut(s) 186, 854, 1367, 1595, 1628
Eco53kI GAGCTC 2 cut(s) 439, 2473
Eco57I CTGAAG 5 cut(s) 544, 944, 954, 1206, 2146
Eco88I CYCGRG 1 cut(s) 1348
EcoICRI GAGCTC 2 cut(s) 439, 2473
EcoNI CCTNNNNNAGG 2 cut(s) 1247, 1673
EcoO109I RGGNCCY 2 cut(s) 1460, 1628
EcoRI GAATTC 2 cut(s) 1997, 2202
EcoRII CCWGG 5 cut(s) 237, 1868, 2154, 2394, 2417
EcoRV GATATC 1 cut(s) 641
EcoT14I CCWWGG 2 cut(s) 2145, 2452
EcoT22I ATGCAT 2 cut(s) 103, 1522
EcoT38I GRGCYC 2 cut(s) 441, 2475
ErhI CCWWGG 2 cut(s) 2145, 2452
FaqI GGGAC 6 cut(s) 170, 229, 364, 986, 1641, 2359
FauI CCCGC 1 cut(s) 604
FauNDI CATATG 2 cut(s) 543, 1600
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 4 cut(s) 526, 565, 1689, 2489
FokI GGATG 3 cut(s) 110, 1277, 1486
FriOI GRGCYC 2 cut(s) 441, 2475
Fsp4HI GCNGC 4 cut(s) 526, 565, 1689, 2489
FspBI CTAG 7 cut(s) 59, 827, 1041, 1152, 1401, 1862, 2321
FspI TGCGCA 1 cut(s) 677
GlaI GCGC 3 cut(s) 288, 524, 677
GluI GCNGC 4 cut(s) 526, 565, 1689, 2489
GsaI CCCAGC 1 cut(s) 1628
GsuI CTGGAG 2 cut(s) 1086, 2489
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 5 cut(s) 567, 1462, 1668, 1706, 2352
HapII CCGG 7 cut(s) 135, 189, 204, 744, 805, 1060, 2387
HhaI GCGC 3 cut(s) 289, 525, 678
Hin6I GCGC 3 cut(s) 287, 523, 676
HinP1I GCGC 3 cut(s) 287, 523, 676
HincII GTYRAC 2 cut(s) 475, 2427
HindII GTYRAC 2 cut(s) 475, 2427
HpaI GTTAAC 1 cut(s) 2427
HpaII CCGG 7 cut(s) 135, 189, 204, 744, 805, 1060, 2387
HphI GGTGA 4 cut(s) 218, 1105, 1308, 2000
Hpy166II GTNNAC 6 cut(s) 464, 475, 1469, 1752, 1842, 2427
Hpy8I GTNNAC 6 cut(s) 464, 475, 1469, 1752, 1842, 2427
Hpy99I CGWCG 1 cut(s) 1804
HpyAV CCTTC 8 cut(s) 109, 568, 929, 1121, 1451, 1794, 2069, 2336
HpyCH4III ACNGT 3 cut(s) 1738, 2066, 2371
HpyCH4IV ACGT 8 cut(s) 210, 281, 380, 460, 811, 2122, 2176, 2361
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 250, 436, 1344, 1526, 2297
HpySE526I ACGT 8 cut(s) 210, 281, 380, 460, 811, 2122, 2176, 2361
HspAI GCGC 3 cut(s) 287, 523, 676
Kpn2I TCCGGA 2 cut(s) 743, 1059
KpnI GGTACC 3 cut(s) 2118, 2395, 2528
KspAI GTTAAC 1 cut(s) 2427
Kzo9I GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 5 cut(s) 206, 241, 785, 2080, 2470
Lsp1109I GCAGC 2 cut(s) 1700, 2475
LweI GCATC 6 cut(s) 88, 1322, 1464, 1563, 1748, 1895
MaeI CTAG 7 cut(s) 59, 827, 1041, 1152, 1401, 1862, 2321
MaeII ACGT 8 cut(s) 210, 281, 380, 460, 811, 2122, 2176, 2361
MaeIII GTNAC 6 cut(s) 76, 206, 812, 1111, 1282, 2123
MalI GATC 8 cut(s) 168, 798, 831, 925, 1056, 1202, 1978, 2400
MbiI CCGCTC 1 cut(s) 599
MboI GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 6 cut(s) 166, 829, 923, 1200, 1976, 2398
MhlI GDGCHC 4 cut(s) 203, 441, 615, 2475
MlyI GAGTC 2 cut(s) 2270, 2304
MmeI TCCRAC 2 cut(s) 684, 2304
Mph1103I ATGCAT 2 cut(s) 103, 1522
MroI TCCGGA 2 cut(s) 743, 1059
MroXI GAANNNNTTC 3 cut(s) 739, 1487, 1586
MslI CAYNNNNRTG 1 cut(s) 1515
MspA1I CMGCKG 3 cut(s) 564, 1691, 1949
MspCI CTTAAG 1 cut(s) 343
MspI CCGG 7 cut(s) 135, 189, 204, 744, 805, 1060, 2387
MspR9I CCNGG 7 cut(s) 136, 239, 1870, 2156, 2388, 2396, 2419
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 5 cut(s) 239, 1870, 2156, 2396, 2419
MvnI CGCG 2 cut(s) 525, 2520
MwoI GCNNNNNNNGC 6 cut(s) 107, 250, 436, 1344, 1526, 2297
NciI CCSGG 2 cut(s) 136, 2388
NdeI CATATG 2 cut(s) 543, 1600
NdeII GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
NheI GCTAGC 1 cut(s) 1151
NmuCI GTSAC 3 cut(s) 206, 1111, 2123
NruI TCGCGA 1 cut(s) 2520
NsbI TGCGCA 1 cut(s) 677
NsiI ATGCAT 2 cut(s) 103, 1522
NspI RCATGY 2 cut(s) 236, 1520
PaeI GCATGC 1 cut(s) 1520
PciSI GCTCTTC 1 cut(s) 606
PctI GAATGC 1 cut(s) 1153
PdmI GAANNNNTTC 3 cut(s) 739, 1487, 1586
PfeI GAWTC 8 cut(s) 131, 139, 268, 718, 841, 1096, 1211, 1582
PflMI CCANNNNNTGG 1 cut(s) 869
PfoI TCCNGGA 2 cut(s) 134, 237
PkrI GCNGC 4 cut(s) 527, 566, 1690, 2490
PleI GAGTC 2 cut(s) 2270, 2304
PpsI GAGTC 2 cut(s) 2270, 2304
Ppu21I YACGTR 5 cut(s) 461, 812, 2123, 2177, 2362
PpuMI RGGWCCY 1 cut(s) 1628
PshAI GACNNNNGTC 1 cut(s) 1798
PsiI TTATAA 2 cut(s) 1697, 1923
Psp124BI GAGCTC 2 cut(s) 441, 2475
Psp1406I AACGTT 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 1628
Psp6I CCWGG 5 cut(s) 237, 1868, 2154, 2394, 2417
PspFI CCCAGC 1 cut(s) 1624
PspGI CCWGG 5 cut(s) 237, 1868, 2154, 2394, 2417
PspPI GGNCC 9 cut(s) 186, 854, 1367, 1460, 1595, 1628, 1666, 1704, 2351
PspPPI RGGWCCY 1 cut(s) 1628
PstI CTGCAG 1 cut(s) 776
PstNI CAGNNNCTG 2 cut(s) 244, 771
PsuI RGATCY 6 cut(s) 166, 829, 923, 1200, 1976, 2398
PvuII CAGCTG 2 cut(s) 1691, 1949
RruI TCGCGA 1 cut(s) 2520
RsaI GTAC 4 cut(s) 1033, 2116, 2393, 2526
RsaNI GTAC 4 cut(s) 1032, 2115, 2392, 2525
RseI CAYNNNNRTG 1 cut(s) 1515
SacI GAGCTC 2 cut(s) 441, 2475
SapI GCTCTTC 1 cut(s) 606
SatI GCNGC 4 cut(s) 526, 565, 1689, 2489
Sau3AI GATC 8 cut(s) 166, 796, 829, 923, 1054, 1200, 1976, 2398
Sau96I GGNCC 9 cut(s) 186, 854, 1367, 1460, 1595, 1628, 1666, 1704, 2351
SchI GAGTC 2 cut(s) 2270, 2304
ScrFI CCNGG 7 cut(s) 136, 239, 1870, 2156, 2388, 2396, 2419
SduI GDGCHC 4 cut(s) 203, 441, 615, 2475
SfaNI GCATC 6 cut(s) 88, 1322, 1464, 1563, 1748, 1895
SfcI CTRYAG 5 cut(s) 772, 1950, 1982, 2367, 2446
SinI GGWCC 5 cut(s) 186, 854, 1367, 1595, 1628
SmiMI CAYNNNNRTG 1 cut(s) 1515
SmlI CTYRAG 1 cut(s) 343
SmoI CTYRAG 1 cut(s) 343
SnaBI TACGTA 4 cut(s) 461, 812, 2177, 2362
SphI GCATGC 1 cut(s) 1520
SsiI CCGC 5 cut(s) 65, 525, 564, 597, 921
SspMI CTAG 7 cut(s) 59, 827, 1041, 1152, 1401, 1862, 2321
SstI GAGCTC 2 cut(s) 441, 2475
StyD4I CCNGG 7 cut(s) 134, 237, 1868, 2154, 2386, 2394, 2417
StyI CCWWGG 2 cut(s) 2145, 2452
TaaI ACNGT 3 cut(s) 1738, 2066, 2371
TaiI ACGT 8 cut(s) 213, 284, 383, 463, 814, 2125, 2179, 2364
TaqI TCGA 3 cut(s) 690, 1611, 2038
TaqII GACCGA 2 cut(s) 871, 2021
TatI WGTACW 1 cut(s) 1031
TauI GCSGC 2 cut(s) 528, 567
TfiI GAWTC 8 cut(s) 131, 139, 268, 718, 841, 1096, 1211, 1582
TscAI CASTG 2 cut(s) 117, 1266
TseFI GTSAC 3 cut(s) 206, 1111, 2123
TseI GCWGC 2 cut(s) 1688, 2488
Tsp45I GTSAC 3 cut(s) 206, 1111, 2123
TspDTI ATGAA 7 cut(s) 319, 560, 596, 1038, 1290, 2092, 2355
TspGWI ACGGA 1 cut(s) 1486
TspRI CASTG 2 cut(s) 117, 1266
Van91I CCANNNNNTGG 1 cut(s) 869
Vha464I CTTAAG 1 cut(s) 343
VpaK11BI GGWCC 5 cut(s) 186, 854, 1367, 1595, 1628
XagI CCTNNNNNAGG 2 cut(s) 1247, 1673
XapI RAATTY 6 cut(s) 570, 1219, 1571, 1997, 2202, 2549
XbaI TCTAGA 1 cut(s) 826
XceI RCATGY 2 cut(s) 236, 1520
XcmI CCANNNNNNNNNTGG 1 cut(s) 2152
XmiI GTMKAC 1 cut(s) 463
XmnI GAANNNNTTC 3 cut(s) 739, 1487, 1586
XspI CTAG 7 cut(s) 59, 827, 1041, 1152, 1401, 1862, 2321
Zsp2I ATGCAT 2 cut(s) 103, 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.