MD12G1192800.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002297
Physical Location & Seq
Forward (+)
27469543 .. 27474966
5424 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1192800.v1.1.491

Sequence Viewer

Length: 2862 bp
ATGCTAAAGCTGAGAGCTTCTGGATGTGTCGTTGCTCTCCTGTGTTCTTGCTTAATGCTGTTTGCCGTTGCAAAAGTTACAAATCCTTCAGAAGTCAATGTACTGAGAGCAGTCAAGAGCAGATTAATTGATCCTAAGAATCATCTAAGGAATTGGGACAACGGGGATCCTTGCGAATCTCATTGGACTGGAGTTTTCTGTTTTGATGCTGTTGGGGATGATGGATACTTGCACCTCCGGGAACTCCAACTGCTGAATATGAATCTCTCAGGAACTTTAGCACCTGAGCTTGGCCAACTATCCCAACTTCTGATATTAGATTTCATGTGGAATGAATTAAGTGGACCTATACCGAAGGAGATCGGAAACATTTCATCCTTGAAGCTCTTGCTCTTGAATGGAAACAAACTATCTGGTTCCTTACCTCCTGAGCTCGGTTATCTTACAAATTTAACTAGACTACAAGTCGATCAGAACTATATGTCAGGTCCAATTCCAAAATCATTTGCTAACATGGCCAGTTTAAAACATTTCCACTTGAACAACAACTCCTTCAGCGGTCGAATTCCACCTGAGCTTTCTAAAGCATCCAATCTTCTTCACTTGCTTTTCGATAATAATAACTTATCCGGATATCTACCACCAGAATTATCCATGTTACCGAATGTGCGCATCATTCAGTGCGATAACAACAACTTCAGTGGGACTGAGATTCCAGCCTCTTATGGAAACCTTTCCAGATTAACAAAACTAAGTCTTAGGAATTGCAGTTTGCAGGGAGAAATTCCTGACTTTAGCAGGATAGCTAACCTTAGTTATATAGATCTAAGCCGGAATCAACTTTCTGGATCCATACCGTCACACAGACTTTCTCACAATATAACAACAATCATTCTGTCAGACAACCATCTTAATGGATCGATTCCTGAGAGTTTTTATGATCTTCCAGCTCTTGAGAAAGTGTCGCTCCACGACAATTTTATTACGGGTTCTGTCCCTGCTATGTGGCGGAATATACCTTTCAGTACAACAGCTAGATTTGCACTTGATCTTCGGAACAATTTGCTTTCACAAATATCGGAAGAGCTAAATCCTCCTGAAAATGTCACTTTGAGGCTTGAAGGAAATCCTGTCTGCAAGAGTGCAAGTGTACCAAATATAGGCCAGTTCTGTCGATCTGAAGCTGGAGGAGACGGGATCTCTGAAAACTCGATAAACTCCACTCAACCAATGACCTGCTCTAGCCAAGAATGTCCAACAGACTATTTCTACGAATATGTCCCATCTTCCCCAGTTCCATGCTTTTGTGCATCACCTTTAAGAGTTGACTATCGTCTAAAAAGTCCGAGTTTTTCATATTTTTCTCCTCATAAACACAATTTTGAAATCTACTTAACTAGTGTTCTCAACCTGCGCCTTTATCAATTATCAATTGATTCATTTGTCTGGCAAGAAGGGCCTCGTTTGCTGATGCATATGAAGTTTTTTCCTATGTTCATCAATCCACACTTGAATTTATTTAATGCAAGTGAGGTTCAGCGATTGAGAGGCCAGCTTACATCATGGGATGTTCCTCCAACTGATTTCTTTGGACCGTATGAGCTTCTCAAATTCACTCTGCTGGGACCTTATTCGAACATGATTGTTGAGCGGCAAAAGGGCATTAGCAAGAGAGCTTTAGCAGGTGTTATAATAGGATGCATTGCTGCTGTTGTCATTATTCCTGCAATAGCCATGCTTGTGACCAAAAGATATGTCAAACACCGTTACCCACCACCATCACGGAGACATTCATCCTTAAAGATCTCTATGAGAATTGAAGGTGTAAAGGCGTTCACTTTCAAAGAAATGAAAGTAGCTACTCAGAATTTCGATACTTCAATGCAACTTGGACGAGGAGGCTATGGGAAAGTTTACAAAGGCATTTTGTCCGATAACACAGCTGTAGCCATAAAGCATGCAGAAGAAGGATCCTTACAGGGCCAAAAGGAGTTCTTGACCGAGATAGAACTGCTATCAAGGGTACATCACCGAAACCTAGTCTCTTTGGTGGGATATTGTGATGAAGAAGGGGAGCAGATGCTGGTTTACGAGTTTATGCCTAATGGTACCTTACACGACTGGCTTTGGGTTAAAGCCAAGGGAAGCCTGGACTTCACTATGAGGTTACGCATTGCATTAGGTTCCGCTAAAGGCATCCTTTATCTCCATACCGAGGCAAATCCTCCAATATTCCACCGGGATATCAAAGCCAGCAACATACTCTTGGACTCCAACCTCGTGGCCAAAGTGGCGGATTTTGGACTCTCGCGACTTGCACCTCCGCAGGATGATGAAGGGACCGGGTCCTCTTATGTATCTACAGTTGTGAAAGGAACGCCGGGTTACATTGATCCAGAGTATTTCCTGACCAATAAGTTGACGGACAAAAGCGATGTCTATAGCCTAGGAATCGTGTTTCTGGAGCTCCTTACCGGCGGTCAGCCAATTTCACACGGCAAAAACATAGTTCGAGAGGTGAATCTGGCTTATCAGGCAGGGCTGGTGTTCTCAATCATAGACAAAAGAATGCGATCCTATCCATCCAAATGTGTTGAGAGGTTTCTAGATTTGGCCCTCAGATGTTGTAACGAAAAGCCAGATAAGCGCCCTCATATGCTGGAAGTGGTGAGGGAGATCGAAAACATACTCAAAATCATGCCGGCAACCGACACCATATTCTCGCCATCTACTTCCTCGTACAGTGACCAGTCACCAACCTCGTCGTCCTTCATGACCGGAGACCGATCCAACATGTCCTCCAGCCTGCTGGCAGGAAGTGATCTAACCAGTGGCGTTGTCCCGACCATAGTGCCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

106.07

Weight (kDa)

7.56

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 67 4.1e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 78 - 278 1.3e-11 Leucine-rich repeat region
Pkinase PF00069 626 - 891 5.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 627 - 892 1.4e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1691
AarI CACCTGC 1 cut(s) 1673
Acc16I TGCGCA 1 cut(s) 671
Acc36I ACCTGC 3 cut(s) 1244, 1419, 1673
Acc65I GGTACC 1 cut(s) 2108
AccB1I GGYRCC 1 cut(s) 2108
AccBSI CCGCTC 1 cut(s) 1651
AccII CGCG 1 cut(s) 2311
AccIII TCCGGA 1 cut(s) 629
AciI CCGC 7 cut(s) 558, 1009, 1651, 2187, 2294, 2324, 2478
AcoI YGGCCR 3 cut(s) 292, 516, 2283
AcsI RAATTY 6 cut(s) 448, 564, 783, 1513, 1610, 1867
AcuI CTGAAG 4 cut(s) 72, 538, 682, 1200
AfaI GTAC 6 cut(s) 102, 1027, 1152, 2025, 2110, 2741
AfiI CCNNNNNNNGG 4 cut(s) 290, 434, 1160, 2215
AflIII ACRYGT 1 cut(s) 2793
AgsI TTSAA 9 cut(s) 382, 397, 541, 1121, 1385, 1513, 1820, 1843, 1881
AhdI GACNNNNNGTC 1 cut(s) 464
AhlI ACTAGT 1 cut(s) 1397
AjnI CCWGG 1 cut(s) 2148
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
Alw21I GWGCWC 2 cut(s) 435, 2469
Alw26I GTCTC 4 cut(s) 1185, 1780, 2047, 2775
AlwNI CAGNNNCTG 1 cut(s) 2083
Aor13HI TCCGGA 1 cut(s) 629
AoxI GGCC 8 cut(s) 292, 516, 1162, 1457, 1549, 1981, 2283, 2613
ApeKI GCWGC 1 cut(s) 1706
ApoI RAATTY 6 cut(s) 448, 564, 783, 1513, 1610, 1867
ArsI GACNNNNNNTTYG 4 cut(s) 1740, 1772, 2421, 2453
AseI ATTAAT 1 cut(s) 125
Asp700I GAANNNNTTC 2 cut(s) 370, 733
Asp718I GGTACC 1 cut(s) 2108
AspA2I CCTAGG 1 cut(s) 2446
AspLEI GCGC 3 cut(s) 672, 1416, 2649
AspS9I GGNCC 9 cut(s) 344, 488, 1457, 1592, 1625, 1981, 2340, 2346, 2614
AsuC2I CCSGG 4 cut(s) 239, 2240, 2344, 2382
AsuHPI GGTGA 5 cut(s) 1305, 2021, 2530, 2680, 2745
AsuII TTCGAA 1 cut(s) 1634
AvaII GGWCC 6 cut(s) 344, 488, 1592, 1625, 2340, 2346
AvrII CCTAGG 1 cut(s) 2446
BalI TGGCCA 3 cut(s) 294, 518, 2285
BamHI GGATCC 3 cut(s) 166, 848, 1970
BanI GGYRCC 1 cut(s) 2108
BanII GRGCYC 2 cut(s) 435, 2469
BarI GAAGNNNNNNTAC 4 cut(s) 84, 116, 1959, 1991
BauI CACGAG 1 cut(s) 2279
Bbv12I GWGCWC 2 cut(s) 435, 2469
BbvI GCAGC 1 cut(s) 1693
BccI CCATC 6 cut(s) 215, 915, 1291, 1786, 2590, 2734
BceAI ACGGC 2 cut(s) 50, 2512
BcgI CGANNNNNNTGC 1 cut(s) 2833
BciT130I CCWGG 1 cut(s) 2150
BciVI GTATCC 1 cut(s) 218
BcnI CCSGG 4 cut(s) 239, 2240, 2344, 2382
BcoDI GTCTC 4 cut(s) 1185, 1780, 2047, 2775
BcuI ACTAGT 1 cut(s) 1397
BfaI CTAG 7 cut(s) 456, 1035, 1242, 1398, 2039, 2447, 2606
BfmI CTRYAG 3 cut(s) 1944, 2361, 2440
BfoI RGCGCY 1 cut(s) 2650
BfuAI ACCTGC 3 cut(s) 1244, 1419, 1673
BfuI GTATCC 1 cut(s) 218
BglI GCCNNNNNGGC 1 cut(s) 2291
BglII AGATCT 2 cut(s) 823, 1803
BisI GCNGC 2 cut(s) 1652, 1707
BlnI CCTAGG 1 cut(s) 2446
BlsI GCNGC 2 cut(s) 1653, 1708
Bme1390I CCNGG 5 cut(s) 239, 2150, 2240, 2344, 2382
Bme18I GGWCC 6 cut(s) 344, 488, 1592, 1625, 2340, 2346
BmeRI GACNNNNNGTC 1 cut(s) 464
BmgT120I GGNCC 9 cut(s) 344, 488, 1457, 1592, 1625, 1981, 2340, 2346, 2614
BmiI GGNNCC 9 cut(s) 168, 418, 850, 1626, 1972, 2110, 2185, 2341, 2347
BmrFI CCNGG 5 cut(s) 239, 2150, 2240, 2344, 2382
BmrI ACTGGG 1 cut(s) 1286
BmsI GCATC 8 cut(s) 196, 596, 681, 1319, 1461, 1688, 2070, 2205
BmuI ACTGGG 1 cut(s) 1286
BoxI GACNNNNGTC 1 cut(s) 1332
BpmI CTGGAG 4 cut(s) 210, 1206, 2483, 2785
Bpu10I CCTNAGC 3 cut(s) 285, 429, 573
Bpu14I TTCGAA 1 cut(s) 1634
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 4 cut(s) 239, 2240, 2344, 2382
Bsa29I ATCGAT 1 cut(s) 920
BsaI GGTCTC 1 cut(s) 2775
BsaJI CCNNGG 3 cut(s) 2139, 2214, 2446
BsaWI WCCGGW 2 cut(s) 629, 2777
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 2783, 2813
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 434, 1160, 2215
Bse118I RCCGGY 2 cut(s) 2474, 2701
Bse1I ACTGG 7 cut(s) 193, 519, 1165, 1292, 2126, 2749, 2829
Bse3DI GCAATG 2 cut(s) 1701, 2172
BseAI TCCGGA 1 cut(s) 629
BseBI CCWGG 1 cut(s) 2150
BseCI ATCGAT 1 cut(s) 920
BseDI CCNNGG 3 cut(s) 2139, 2214, 2446
BseLI CCNNNNNNNGG 4 cut(s) 290, 434, 1160, 2215
BseMI GCAATG 2 cut(s) 1701, 2172
BseMII CTCAG 9 cut(s) 95, 276, 282, 420, 564, 699, 918, 1877, 2632
BseNI ACTGG 7 cut(s) 193, 519, 1165, 1292, 2126, 2749, 2829
BseRI GAGGAG 3 cut(s) 1203, 1356, 1911
BseXI GCAGC 1 cut(s) 1693
BseYI CCCAGC 1 cut(s) 1621
Bsh1236I CGCG 1 cut(s) 2311
Bsh1285I CGRYCG 1 cut(s) 562
BshFI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 1983, 2285, 2615
BshNI GGYRCC 1 cut(s) 2108
BshVI ATCGAT 1 cut(s) 920
BsiEI CGRYCG 1 cut(s) 562
BsiHKAI GWGCWC 2 cut(s) 435, 2469
BsiSI CCGG 9 cut(s) 238, 630, 832, 2239, 2343, 2381, 2475, 2702, 2778
BslFI GGGAC 7 cut(s) 170, 718, 980, 1265, 1638, 2353, 2825
BslI CCNNNNNNNGG 4 cut(s) 290, 434, 1160, 2215
BsmAI GTCTC 4 cut(s) 1185, 1780, 2047, 2775
BsmBI CGTCTC 1 cut(s) 1185
BsmFI GGGAC 7 cut(s) 170, 718, 980, 1265, 1638, 2353, 2825
BsmI GAATGC 1 cut(s) 2574
BsnI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 1983, 2285, 2615
Bso31I GGTCTC 1 cut(s) 2775
Bsp119I TTCGAA 1 cut(s) 1634
Bsp1286I GDGCHC 2 cut(s) 435, 2469
Bsp13I TCCGGA 1 cut(s) 629
Bsp68I TCGCGA 1 cut(s) 2311
BspACI CCGC 7 cut(s) 558, 1009, 1651, 2187, 2294, 2324, 2478
BspANI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 1983, 2285, 2615
BspCNI CTCAG 9 cut(s) 96, 277, 281, 421, 565, 700, 919, 1876, 2631
BspDI ATCGAT 1 cut(s) 920
BspEI TCCGGA 1 cut(s) 629
BspFNI CGCG 1 cut(s) 2311
BspHI TCATGA 1 cut(s) 2772
BspLI GGNNCC 9 cut(s) 168, 418, 850, 1626, 1972, 2110, 2185, 2341, 2347
BspMI ACCTGC 3 cut(s) 1244, 1419, 1673
BspQI GCTCTTC 1 cut(s) 1077
BspT104I TTCGAA 1 cut(s) 1634
BspT107I GGYRCC 1 cut(s) 2108
BspTNI GGTCTC 1 cut(s) 2775
BsrBI CCGCTC 1 cut(s) 1651
BsrDI GCAATG 2 cut(s) 1701, 2172
BsrFI RCCGGY 2 cut(s) 2474, 2701
BsrI ACTGG 7 cut(s) 193, 519, 1165, 1292, 2126, 2749, 2829
BssAI RCCGGY 2 cut(s) 2474, 2701
BssECI CCNNGG 3 cut(s) 2139, 2214, 2446
BssSI CACGAG 1 cut(s) 2279
BssT1I CCWWGG 2 cut(s) 2139, 2446
Bst2BI CACGAG 1 cut(s) 2279
Bst2UI CCWGG 1 cut(s) 2150
Bst4CI ACNGT 5 cut(s) 858, 1596, 1766, 2365, 2744
Bst6I CTCTTC 1 cut(s) 1077
BstBI TTCGAA 1 cut(s) 1634
BstC8I GCNNGC 6 cut(s) 1553, 1959, 2254, 2703, 2807, 2811
BstFNI CGCG 1 cut(s) 2311
BstH2I RGCGCY 1 cut(s) 2650
BstHHI GCGC 3 cut(s) 672, 1416, 2649
BstMAI GTCTC 4 cut(s) 1185, 1780, 2047, 2775
BstMCI CGRYCG 1 cut(s) 562
BstMWI GCNNNNNNNGC 7 cut(s) 515, 1040, 1456, 1465, 2291, 2534, 2644
BstNI CCWGG 1 cut(s) 2150
BstNSI RCATGY 2 cut(s) 1961, 2797
BstPAI GACNNNNGTC 1 cut(s) 1332
BstSCI CCNGG 5 cut(s) 237, 2148, 2238, 2342, 2380
BstSFI CTRYAG 3 cut(s) 1944, 2361, 2440
BstUI CGCG 1 cut(s) 2311
BstV1I GCAGC 1 cut(s) 1693
BstX2I RGATCY 6 cut(s) 166, 823, 848, 1197, 1803, 1970
BstXI CCANNNNNNTGG 3 cut(s) 914, 2281, 2809
BstYI RGATCY 6 cut(s) 166, 823, 848, 1197, 1803, 1970
Bsu15I ATCGAT 1 cut(s) 920
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 1983, 2285, 2615
BsuTUI ATCGAT 1 cut(s) 920
BtgZI GCGATG 1 cut(s) 2448
BtsIMutI CAGTG 4 cut(s) 686, 706, 2749, 2836
BtuMI TCGCGA 1 cut(s) 2311
BveI ACCTGC 3 cut(s) 1244, 1419, 1673
Cac8I GCNNGC 6 cut(s) 1553, 1959, 2254, 2703, 2807, 2811
CaiI CAGNNNCTG 1 cut(s) 2083
CciI TCATGA 1 cut(s) 2772
CfoI GCGC 3 cut(s) 672, 1416, 2649
Cfr10I RCCGGY 2 cut(s) 2474, 2701
Cfr13I GGNCC 9 cut(s) 344, 488, 1457, 1592, 1625, 1981, 2340, 2346, 2614
ClaI ATCGAT 1 cut(s) 920
Csp6I GTAC 6 cut(s) 101, 1026, 1151, 2024, 2109, 2740
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviQI GTAC 6 cut(s) 101, 1026, 1151, 2024, 2109, 2740
DraI TTTAAA 1 cut(s) 525
DriI GACNNNNNGTC 1 cut(s) 464
EaeI YGGCCR 3 cut(s) 292, 516, 2283
Eam1104I CTCTTC 1 cut(s) 1077
Eam1105I GACNNNNNGTC 1 cut(s) 464
EarI CTCTTC 1 cut(s) 1077
EciI GGCGGA 2 cut(s) 1024, 2309
Ecl136II GAGCTC 2 cut(s) 433, 2467
Eco130I CCWWGG 2 cut(s) 2139, 2446
Eco24I GRGCYC 2 cut(s) 435, 2469
Eco31I GGTCTC 1 cut(s) 2775
Eco32I GATATC 2 cut(s) 635, 2245
Eco47I GGWCC 6 cut(s) 344, 488, 1592, 1625, 2340, 2346
Eco53kI GAGCTC 2 cut(s) 433, 2467
Eco57I CTGAAG 4 cut(s) 72, 538, 682, 1200
EcoICRI GAGCTC 2 cut(s) 433, 2467
EcoO109I RGGNCCY 3 cut(s) 1457, 1625, 2346
EcoRI GAATTC 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 2148
EcoRV GATATC 2 cut(s) 635, 2245
EcoT14I CCWWGG 2 cut(s) 2139, 2446
EcoT22I ATGCAT 2 cut(s) 1476, 1703
EcoT38I GRGCYC 2 cut(s) 435, 2469
ErhI CCWWGG 2 cut(s) 2139, 2446
Esp3I CGTCTC 1 cut(s) 1185
FalI AAGNNNNNCTT 4 cut(s) 1979, 2011, 2184, 2216
FaqI GGGAC 7 cut(s) 170, 718, 980, 1265, 1638, 2353, 2825
FauNDI CATATG 2 cut(s) 1476, 2655
Fnu4HI GCNGC 2 cut(s) 1652, 1707
FriOI GRGCYC 2 cut(s) 435, 2469
Fsp4HI GCNGC 2 cut(s) 1652, 1707
FspAI RTGCGCAY 1 cut(s) 671
FspBI CTAG 7 cut(s) 456, 1035, 1242, 1398, 2039, 2447, 2606
FspI TGCGCA 1 cut(s) 671
GlaI GCGC 3 cut(s) 671, 1415, 2648
GluI GCNGC 2 cut(s) 1652, 1707
GsaI CCCAGC 1 cut(s) 1625
GsuI CTGGAG 4 cut(s) 210, 1206, 2483, 2785
HaeII RGCGCY 1 cut(s) 2650
HaeIII GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 1983, 2285, 2615
HapII CCGG 9 cut(s) 238, 630, 832, 2239, 2343, 2381, 2475, 2702, 2778
HhaI GCGC 3 cut(s) 672, 1416, 2649
Hin6I GCGC 3 cut(s) 670, 1414, 2647
HinP1I GCGC 3 cut(s) 670, 1414, 2647
HincII GTYRAC 2 cut(s) 1327, 2421
HindII GTYRAC 2 cut(s) 1327, 2421
HpaII CCGG 9 cut(s) 238, 630, 832, 2239, 2343, 2381, 2475, 2702, 2778
HphI GGTGA 5 cut(s) 1305, 2021, 2530, 2680, 2745
Hpy166II GTNNAC 7 cut(s) 344, 1151, 1327, 1836, 1915, 2089, 2421
Hpy8I GTNNAC 7 cut(s) 344, 1151, 1327, 1836, 1915, 2089, 2421
Hpy99I CGWCG 1 cut(s) 2767
HpyCH4III ACNGT 5 cut(s) 858, 1596, 1766, 2365, 2744
HpyF10VI GCNNNNNNNGC 7 cut(s) 515, 1040, 1456, 1465, 2291, 2534, 2644
HspAI GCGC 3 cut(s) 670, 1414, 2647
Kpn2I TCCGGA 1 cut(s) 629
KpnI GGTACC 1 cut(s) 2112
KroI GCCGGC 1 cut(s) 2701
KroNI GCCGGC 1 cut(s) 2703
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 4 cut(s) 972, 2074, 2464, 2472
Lsp1109I GCAGC 1 cut(s) 1693
LweI GCATC 8 cut(s) 196, 596, 681, 1319, 1461, 1688, 2070, 2205
MaeI CTAG 7 cut(s) 456, 1035, 1242, 1398, 2039, 2447, 2606
MbiI CCGCTC 1 cut(s) 1651
MboII GAAGA 8 cut(s) 587, 590, 935, 1043, 1094, 1278, 1976, 2078
MfeI CAATTG 1 cut(s) 1431
MflI RGATCY 6 cut(s) 166, 823, 848, 1197, 1803, 1970
MhlI GDGCHC 2 cut(s) 435, 2469
MlsI TGGCCA 3 cut(s) 294, 518, 2285
MluNI TGGCCA 3 cut(s) 294, 518, 2285
MlyI GAGTC 2 cut(s) 2264, 2298
MmeI TCCRAC 5 cut(s) 271, 1280, 1601, 2298, 2814
Mox20I TGGCCA 3 cut(s) 294, 518, 2285
Mph1103I ATGCAT 2 cut(s) 1476, 1703
MroI TCCGGA 1 cut(s) 629
MroNI GCCGGC 1 cut(s) 2701
MroXI GAANNNNTTC 2 cut(s) 370, 733
MscI TGGCCA 3 cut(s) 294, 518, 2285
MslI CAYNNNNRTG 3 cut(s) 912, 1739, 2587
Msp20I TGGCCA 3 cut(s) 294, 518, 2285
MspA1I CMGCKG 2 cut(s) 558, 1943
MspI CCGG 9 cut(s) 238, 630, 832, 2239, 2343, 2381, 2475, 2702, 2778
MspR9I CCNGG 5 cut(s) 239, 2150, 2240, 2344, 2382
MunI CAATTG 1 cut(s) 1431
Mva1269I GAATGC 1 cut(s) 2574
MvaI CCWGG 1 cut(s) 2150
MvnI CGCG 1 cut(s) 2311
MwoI GCNNNNNNNGC 7 cut(s) 515, 1040, 1456, 1465, 2291, 2534, 2644
NaeI GCCGGC 1 cut(s) 2703
NciI CCSGG 4 cut(s) 239, 2240, 2344, 2382
NdeI CATATG 2 cut(s) 1476, 2655
NgoMIV GCCGGC 1 cut(s) 2701
NlaIV GGNNCC 9 cut(s) 168, 418, 850, 1626, 1972, 2110, 2185, 2341, 2347
NmuCI GTSAC 5 cut(s) 858, 1105, 1741, 2744, 2751
NruI TCGCGA 1 cut(s) 2311
NsbI TGCGCA 1 cut(s) 671
NsiI ATGCAT 2 cut(s) 1476, 1703
NspI RCATGY 2 cut(s) 1961, 2797
NspV TTCGAA 1 cut(s) 1634
PaeI GCATGC 1 cut(s) 1961
PagI TCATGA 1 cut(s) 2772
PaqCI CACCTGC 1 cut(s) 1673
PciI ACATGT 1 cut(s) 2793
PciSI GCTCTTC 1 cut(s) 1077
PctI GAATGC 1 cut(s) 2574
PdiI GCCGGC 1 cut(s) 2703
PdmI GAANNNNTTC 2 cut(s) 370, 733
PfeI GAWTC 9 cut(s) 139, 176, 262, 712, 835, 922, 1436, 2451, 2521
PflFI GACNNNGTC 1 cut(s) 2344
PfoI TCCNGGA 1 cut(s) 237
PkrI GCNGC 2 cut(s) 1653, 1708
PleI GAGTC 2 cut(s) 2264, 2298
PpsI GAGTC 2 cut(s) 2264, 2298
PpuMI RGGWCCY 2 cut(s) 1625, 2346
PscI ACATGT 1 cut(s) 2793
PshAI GACNNNNGTC 1 cut(s) 1332
PshBI ATTAAT 1 cut(s) 125
PsiI TTATAA 1 cut(s) 1691
Psp124BI GAGCTC 2 cut(s) 435, 2469
Psp5II RGGWCCY 2 cut(s) 1625, 2346
Psp6I CCWGG 1 cut(s) 2148
PspFI CCCAGC 1 cut(s) 1621
PspGI CCWGG 1 cut(s) 2148
PspN4I GGNNCC 9 cut(s) 168, 418, 850, 1626, 1972, 2110, 2185, 2341, 2347
PspPI GGNCC 9 cut(s) 344, 488, 1457, 1592, 1625, 1981, 2340, 2346, 2614
PspPPI RGGWCCY 2 cut(s) 1625, 2346
PstNI CAGNNNCTG 1 cut(s) 2083
PsuI RGATCY 6 cut(s) 166, 823, 848, 1197, 1803, 1970
PsyI GACNNNGTC 1 cut(s) 2344
PvuII CAGCTG 1 cut(s) 1943
RruI TCGCGA 1 cut(s) 2311
RsaI GTAC 6 cut(s) 102, 1027, 1152, 2025, 2110, 2741
RsaNI GTAC 6 cut(s) 101, 1026, 1151, 2024, 2109, 2740
RseI CAYNNNNRTG 3 cut(s) 912, 1739, 2587
SacI GAGCTC 2 cut(s) 435, 2469
SapI GCTCTTC 1 cut(s) 1077
SatI GCNGC 2 cut(s) 1652, 1707
Sau96I GGNCC 9 cut(s) 344, 488, 1457, 1592, 1625, 1981, 2340, 2346, 2614
SchI GAGTC 2 cut(s) 2264, 2298
ScrFI CCNGG 5 cut(s) 239, 2150, 2240, 2344, 2382
SduI GDGCHC 2 cut(s) 435, 2469
SfaNI GCATC 8 cut(s) 196, 596, 681, 1319, 1461, 1688, 2070, 2205
SfcI CTRYAG 3 cut(s) 1944, 2361, 2440
SfuI TTCGAA 1 cut(s) 1634
SinI GGWCC 6 cut(s) 344, 488, 1592, 1625, 2340, 2346
SmiMI CAYNNNNRTG 3 cut(s) 912, 1739, 2587
SmlI CTYRAG 1 cut(s) 953
SmoI CTYRAG 1 cut(s) 953
SpeI ACTAGT 1 cut(s) 1397
SphI GCATGC 1 cut(s) 1961
SsiI CCGC 7 cut(s) 558, 1009, 1651, 2187, 2294, 2324, 2478
SspI AATATT 1 cut(s) 2232
SspMI CTAG 7 cut(s) 456, 1035, 1242, 1398, 2039, 2447, 2606
SstI GAGCTC 2 cut(s) 435, 2469
StyD4I CCNGG 5 cut(s) 237, 2148, 2238, 2342, 2380
StyI CCWWGG 2 cut(s) 2139, 2446
TaaI ACNGT 5 cut(s) 858, 1596, 1766, 2365, 2744
TaqII GACCGA 2 cut(s) 2015, 2799
TatI WGTACW 2 cut(s) 100, 1025
TauI GCSGC 1 cut(s) 1654
TfiI GAWTC 9 cut(s) 139, 176, 262, 712, 835, 922, 1436, 2451, 2521
TscAI CASTG 4 cut(s) 686, 706, 2749, 2836
TseFI GTSAC 5 cut(s) 858, 1105, 1741, 2744, 2751
TseI GCWGC 1 cut(s) 1706
Tsp45I GTSAC 5 cut(s) 858, 1105, 1741, 2744, 2751
TspGWI ACGGA 2 cut(s) 1798, 2438
TspRI CASTG 4 cut(s) 686, 706, 2749, 2836
Tth111I GACNNNGTC 1 cut(s) 2344
VpaK11BI GGWCC 6 cut(s) 344, 488, 1592, 1625, 2340, 2346
VspI ATTAAT 1 cut(s) 125
XapI RAATTY 6 cut(s) 448, 564, 783, 1513, 1610, 1867
XbaI TCTAGA 1 cut(s) 2605
XceI RCATGY 2 cut(s) 1961, 2797
XcmI CCANNNNNNNNNTGG 1 cut(s) 2146
XmaJI CCTAGG 1 cut(s) 2446
XmnI GAANNNNTTC 2 cut(s) 370, 733
XspI CTAG 7 cut(s) 456, 1035, 1242, 1398, 2039, 2447, 2606
Zsp2I ATGCAT 2 cut(s) 1476, 1703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.