RLG00000025050
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002297
Physical Location & Seq
Forward (+)
41594239 .. 41602998
8760 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025050

Sequence Viewer

Length: 3063 bp
ATGTTGAGACTGAGATTTTGGGGTTGCCTCATTGGTCTGGCTTGTTCTTGCTTCTTCCTAGTTGGGGTTGCACAAGTTACTAATCCTTCTGAAGTGGATGCATTGAGAGCAGTGAAGGGTAGTTTGATTGATTCCGGGAAGCATTTGAGGAATTGGGACAAAGGGGATCCATGTAAAGCGAATTGGACCGGAGTTTTGTGTTCCGGTGATGTTGGGACTGATGGATACTTGCATGTCCAAGAGCTGCAACTGCTGAGCATGAATCTCTCAGGAACTTTAGCGCCTGAGCTTGGCAAATTATCTCAACTTCTGATATTAGATTTCATGTGGAATAACTTAAGTGGGACTATACCGAAGGAGATAGGAAACATCACGTCCTTGAAACTCTTGCTCTTGAATGGAAACAAACTATCGGGTTCCTTGCCTGATGAGCTCGGTTCTCTTGAAAATCTACATAGACTACAAGTTGACGAGAACCATCTTTCAGGTTCAATACCAAAATCATTTGTTAACTTGCGTGGCTTAAAACACCTCCATATGAACAACAACTCCTTCAGTGGCCAAATCCCATCTGAGATTTCTGAAATACCCGTTCTTCTTCATGTGCTTCTGGATAATAATAACTTATCTGGAAATCTTCCACCAGAATTCTCCAACATATCAGAGTTGCGCATACTTCAACTCGATAACAACAACTTCACGGCAACTGAGATTCCAGCTACTTATGGAAACCTTTCCAGATTGGCAAAGTTAAGTCTCAGGAACTGCAGTTTGCAGGGAGCAATTCCTGATCTTAGCCGGATACGTAACCTCAGTTATCTAGATCTAAGCTGGAATCATCTTGCTGGACCGATACCATCACATGGACTTTCTGACAATATGACAACAATTGTTCTGTCAGATAATCATCTTAACGGATCTATTCCTGAAGGTTTCTCTCATTTTTCTCGTCTTCAGAAATTATCGCTCAAAAATAATCAGTTGAACGGTTCTGTCCCTGCTATGTGGAGGAACATATCTTTTAGTGCAAGAGCTAGACTTTCATTTGATCTCCGGAACAATTCACTCTCTAACATATTGGGAGAGCTGAATCCTCCAGCAAATGTCACCTTGAGGCTAGAAGGAAATCCTATTTGCAAGAATGCTAGCATAGAAAACAGAAGCCTGTTCTGTCAATCTGAAGCGAGAGATGGGATCCCCGAAGATTCAATAAATTCAACTCAACAGATGACCTGCCTCATTCAGGAATGTCCAGTGGATGATTTCTATGAATATGTTCCATCTTCCCCTGTGCCATGCTTTTGTGCATCACCTATTATAGTTGAATATCGCCTGAAAAGCCCGAGCTTTTCTTACTTTGGTCCATATATACAGCAGTTTGAAGAGTATTTAACTAATTTTCTAAAATTGAAGATTTACCAGTTATCAATTAGTTCCTTTGTCTGGGAAAAAGGGCCTCGTTTACGGATGCAGTTGAAGTTCTTCCCTGTGTATGGTGCTTCACACTCGCATGCATTAAATGCAAGTGAGGTTCTGCGAATTAGAGGCATCTTTACATCATGGGAATTTCCTCAGAATCATTTCTTTGGACCATATGAGCTACTCGACTTCAAACTGCTGGGACCTTATTCAAATATGATTGTTAAGAGACAAAAGATGGGCATCAGTAAGGGTGTATTAGCAGCTGTTATAATAGGGACCATTGCTTCTGTTGTCAGTATGACTGCAACAGTCATGCTTCTGTTCACAAGATGCACCAGAAACCGATACACGCCACCTTCAAGGAGACACTCATCGTGGAAGATTTCTATGAGAATTGATGGTGTAAAGGCGTTCACTTTCAAAGAAATGACACTAGCCACCAGGAACTTTGATAGTGCATCTCAACTTGGACGAGGAGGCTATGGGAAAGTTTATAAAGGTATCTTATCTGATAACACAGCTGTAGCCATAAAACGTGCAGAAGCAGGATCCCTACAGGGTGAAAGAGAATTCTTGACCGAGATACAATTATTATCAAGGCTACACCATCGAAACTTAGTCTCTTTGGTAGGATACTGTGAAGAAGAAGGGGAGCAGATGTTGGTTTATGAATTCATGCCTAATGGTACCTTACGTGACTGGCTTTCTGATAAAACTAAGGGAAACCTGGACTTCAGTATGAGATTACGTATTGCATTGGGTTCAGCCAAAGGAATTCTTTATCTCCATACCGAGGCAAATCCTCCTATATTCCACCGAGATATTAAAGCCACAAATATACTTTTGGACTCCAACTATATCGCTAAGGTGGCCGATTTTGGACTCTCACGACTAGCACCTGTCCAGAATGATGAAGGGACTGGCCCTGCATACGTATCTACAGTCGTGAGAGGAACACCGGGCTACCTGGATCCAGAATATTTCTTGACCAGAAAGTTAACAGACAAAAGCGATGTCTATAGCCTTGGAATTGTGTTTCTGGAGCTCTTGACTGGTGTGCTGCCAATATCACATGGCAAAAACATTGTTCGCGAGGTGAATATGGCTCATCAGTCAGGCTTGATGTTCTCCATTATAGATGGCAGAATGGGTTCGTATCCATCTGAATGTGTTCAGAGGTTTGTAGCTTTGGCCCTCAGATGTTGCCATGATAAACAAGACGACCGGCCATCAATGTTGGATGTGGTGAGGGAGCTCGAGAACATAATCAAACTTATGCCGGAAGCTGAAACAATGTTTTCACCATCTGGTTCCTCATACACTGAAAAATCATTAACTTCATCATCGACTTTTACTGGGGACCTATATACGTCCTCCTCCAACGTGGAAGGAAGTGATCTAACCAGTGGTGTAATCCCTACCATCTCGCCTCGTTCATTATTCCCCGGGAAACCTTTGGAATTGCGTTTGACTGACGTTGAGTCAAGCCCTTCCCTTTCTCTCTCTCTCTCTCTCTCGCTTGGTGGGTGCTATCGGGGGACACCAGGGCACATCAAAGTATCAAACCACGAGGGGTGGCTTCGAACTGGAGAAGTGACCTTGGATGGTTTGATAGAGCGCAGCATATCTGATAGCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1021

Amino Acids

113.02

Weight (kDa)

7.1

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 2.4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 92 - 182 3.8e-08 Leucine-rich repeat region
LRR_14 PF23598 169 - 281 5.1e-08 Leucine-rich repeat region
Pkinase PF00069 626 - 891 9.7e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 628 - 893 1.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1691, 1917
Acc16I TGCGCA 1 cut(s) 671
Acc36I ACCTGC 1 cut(s) 1241
Acc65I GGTACC 1 cut(s) 2108
AccB1I GGYRCC 1 cut(s) 2108
AccB7I CCANNNNNTGG 1 cut(s) 863
AccII CGCG 1 cut(s) 2514
AccIII TCCGGA 1 cut(s) 1053
AclWI GGATC 9 cut(s) 161, 174, 925, 1189, 1202, 1965, 1978, 2387, 2400
AcoI YGGCCR 3 cut(s) 559, 2292, 2648
AcsI RAATTY 6 cut(s) 647, 1213, 1565, 1991, 2093, 2196
AcuI CTGAAG 6 cut(s) 111, 538, 938, 948, 1200, 2140
AfaI GTAC 1 cut(s) 2110
AfiI CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1243, 1443, 1493, 2215
AflII CTTAAG 1 cut(s) 337
AhdI GACNNNNNGTC 1 cut(s) 2902
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 4 cut(s) 1862, 2148, 2388, 2965
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
Alw21I GWGCWC 3 cut(s) 435, 2469, 2679
Alw26I GTCTC 4 cut(s) 761, 1642, 1780, 2047
AlwI GGATC 9 cut(s) 161, 174, 925, 1189, 1202, 1965, 1978, 2387, 2400
AlwNI CAGNNNCTG 1 cut(s) 765
Ama87I CYCGRG 3 cut(s) 1342, 2678, 2867
Aor13HI TCCGGA 1 cut(s) 1053
AoxI GGCC 6 cut(s) 559, 1454, 2292, 2344, 2613, 2648
ApeKI GCWGC 4 cut(s) 244, 1682, 2482, 3042
ApoI RAATTY 6 cut(s) 647, 1213, 1565, 1991, 2093, 2196
ArsI GACNNNNNNTTYG 5 cut(s) 32, 178, 210, 2421, 2453
Asp700I GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2572, 2592
Asp718I GGTACC 1 cut(s) 2108
AspLEI GCGC 3 cut(s) 283, 672, 3042
AsuC2I CCSGG 4 cut(s) 136, 2382, 2868, 2869
AsuHPI GGTGA 7 cut(s) 218, 1099, 1302, 1994, 2530, 2680, 2715
AsuII TTCGAA 1 cut(s) 3004
AsuNHI GCTAGC 1 cut(s) 1145
AvaI CYCGRG 3 cut(s) 1342, 2678, 2867
AvaII GGWCC 7 cut(s) 186, 848, 1361, 1589, 1622, 1698, 2782
BaeGI GKGCMC 1 cut(s) 2973
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 4 cut(s) 166, 1194, 1970, 2392
BanI GGYRCC 1 cut(s) 2108
BanII GRGCYC 3 cut(s) 435, 2469, 2679
BauI CACGAG 1 cut(s) 2990
BbsI GAAGAC 1 cut(s) 944
Bbv12I GWGCWC 3 cut(s) 435, 2469, 2679
BbvI GCAGC 4 cut(s) 231, 1694, 2469, 3054
BceAI ACGGC 1 cut(s) 717
BciT130I CCWGG 4 cut(s) 1864, 2150, 2390, 2967
BciVI GTATCC 4 cut(s) 218, 795, 2048, 2589
BcnI CCSGG 4 cut(s) 136, 2382, 2868, 2869
BcoDI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BfaI CTAG 7 cut(s) 59, 821, 1035, 1118, 1146, 1856, 2315
BfmI CTRYAG 5 cut(s) 766, 1944, 1976, 2361, 2440
BfoI RGCGCY 1 cut(s) 284
BfrI CTTAAG 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 1241
BfuI GTATCC 4 cut(s) 218, 795, 2048, 2589
BglII AGATCT 1 cut(s) 823
BisI GCNGC 4 cut(s) 245, 1683, 2483, 3043
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 4 cut(s) 246, 1684, 2484, 3044
Bme1390I CCNGG 8 cut(s) 136, 1864, 2150, 2382, 2390, 2868, 2869, 2967
Bme18I GGWCC 7 cut(s) 186, 848, 1361, 1589, 1622, 1698, 2782
BmeRI GACNNNNNGTC 1 cut(s) 2902
BmeT110I CYCGRG 3 cut(s) 1342, 2678, 2867
BmgBI CACGTC 1 cut(s) 375
BmrFI CCNGG 8 cut(s) 136, 1864, 2150, 2382, 2390, 2868, 2869, 2967
BmrI ACTGGG 1 cut(s) 2787
BmsI GCATC 7 cut(s) 88, 1316, 1458, 1557, 1671, 1742, 1889
BmtI GCTAGC 1 cut(s) 1149
BmuI ACTGGG 1 cut(s) 2787
BpiI GAAGAC 1 cut(s) 944
BpmI CTGGAG 3 cut(s) 1080, 2483, 3030
Bpu10I CCTNAGC 2 cut(s) 285, 2286
Bpu1102I GCTNAGC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 3004
BpuEI CTTGAG 1 cut(s) 1132
BpuMI CCSGG 4 cut(s) 136, 2382, 2868, 2869
BsaAI YACGTR 4 cut(s) 806, 2117, 2171, 2356
BsaBI GATNNNNATC 2 cut(s) 906, 1661
BsaJI CCNNGG 6 cut(s) 2214, 2446, 2866, 2867, 2966, 3021
BsaWI WCCGGW 3 cut(s) 188, 203, 1053
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 2066, 2096
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1243, 1443, 1493, 2215
Bse118I RCCGGY 1 cut(s) 2646
Bse1I ACTGG 8 cut(s) 1253, 1420, 2126, 2347, 2479, 2782, 2826, 3013
Bse3DI GCAATG 1 cut(s) 1701
Bse8I GATNNNNATC 2 cut(s) 906, 1661
BseAI TCCGGA 1 cut(s) 1053
BseBI CCWGG 4 cut(s) 1864, 2150, 2390, 2967
BseDI CCNNGG 6 cut(s) 2214, 2446, 2866, 2867, 2966, 3021
BseGI GGATG 5 cut(s) 103, 1264, 1473, 2668, 3031
BseJI GATNNNNATC 2 cut(s) 906, 1661
BseLI CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1243, 1443, 1493, 2215
BseMI GCAATG 1 cut(s) 1701
BseMII CTCAG 9 cut(s) 245, 276, 282, 564, 699, 772, 826, 1586, 2632
BseNI ACTGG 8 cut(s) 1253, 1420, 2126, 2347, 2479, 2782, 2826, 3013
BseRI GAGGAG 2 cut(s) 1911, 2788
BseSI GKGCMC 1 cut(s) 2973
BseXI GCAGC 4 cut(s) 231, 1694, 2469, 3054
BseYI CCCAGC 1 cut(s) 1618
BsgI GTGCAG 1 cut(s) 1980
Bsh1236I CGCG 1 cut(s) 2514
Bsh1285I CGRYCG 1 cut(s) 2647
BshFI GGCC 6 cut(s) 561, 1456, 2294, 2346, 2615, 2650
BshNI GGYRCC 1 cut(s) 2108
BsiEI CGRYCG 1 cut(s) 2647
BsiHKAI GWGCWC 3 cut(s) 435, 2469, 2679
BsiHKCI CYCGRG 3 cut(s) 1342, 2678, 2867
BsiSI CCGG 9 cut(s) 135, 189, 204, 799, 1054, 2381, 2647, 2702, 2868
BslFI GGGAC 9 cut(s) 170, 229, 358, 980, 1635, 1711, 2353, 2795, 2974
BslI CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1243, 1443, 1493, 2215
BsmAI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BsmFI GGGAC 9 cut(s) 170, 229, 358, 980, 1635, 1711, 2353, 2795, 2974
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 6 cut(s) 561, 1456, 2294, 2346, 2615, 2650
BsoBI CYCGRG 3 cut(s) 1342, 2678, 2867
Bsp119I TTCGAA 1 cut(s) 3004
Bsp1286I GDGCHC 4 cut(s) 435, 2469, 2679, 2973
Bsp13I TCCGGA 1 cut(s) 1053
Bsp143I GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
Bsp1720I GCTNAGC 1 cut(s) 254
Bsp68I TCGCGA 1 cut(s) 2514
BspANI GGCC 6 cut(s) 561, 1456, 2294, 2346, 2615, 2650
BspCNI CTCAG 9 cut(s) 246, 277, 281, 565, 700, 771, 825, 1585, 2631
BspEI TCCGGA 1 cut(s) 1053
BspFNI CGCG 1 cut(s) 2514
BspMAI CTGCAG 1 cut(s) 770
BspMI ACCTGC 1 cut(s) 1241
BspOI GCTAGC 1 cut(s) 1149
BspPI GGATC 9 cut(s) 161, 174, 925, 1189, 1202, 1965, 1978, 2387, 2400
BspT104I TTCGAA 1 cut(s) 3004
BspT107I GGYRCC 1 cut(s) 2108
BspTI CTTAAG 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 1701
BsrFI RCCGGY 1 cut(s) 2646
BsrI ACTGG 8 cut(s) 1253, 1420, 2126, 2347, 2479, 2782, 2826, 3013
BssAI RCCGGY 1 cut(s) 2646
BssECI CCNNGG 6 cut(s) 2214, 2446, 2866, 2867, 2966, 3021
BssMI GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
BssSI CACGAG 1 cut(s) 2990
BssT1I CCWWGG 2 cut(s) 2446, 3021
Bst2BI CACGAG 1 cut(s) 2990
Bst2UI CCWGG 4 cut(s) 1864, 2150, 2390, 2967
Bst4CI ACNGT 4 cut(s) 989, 1732, 2060, 2365
Bst6I CTCTTC 1 cut(s) 1377
BstAFI CTTAAG 1 cut(s) 337
BstAPI GCANNNNNTGC 1 cut(s) 1520
BstBAI YACGTR 4 cut(s) 806, 2117, 2171, 2356
BstBI TTCGAA 1 cut(s) 3004
BstC8I GCNNGC 2 cut(s) 1147, 1512
BstENI CCTNNNNNAGG 1 cut(s) 1241
BstF5I GGATG 5 cut(s) 103, 1264, 1473, 2668, 3031
BstFNI CGCG 1 cut(s) 2514
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 3 cut(s) 283, 672, 3042
BstKTI GATC 9 cut(s) 169, 793, 826, 920, 1051, 1197, 1973, 2395, 2821
BstMAI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BstMBI GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
BstMCI CGRYCG 1 cut(s) 2647
BstMWI GCNNNNNNNGC 6 cut(s) 107, 250, 430, 1338, 1520, 2291
BstNI CCWGG 4 cut(s) 1864, 2150, 2390, 2967
BstNSI RCATGY 2 cut(s) 236, 1514
BstSCI CCNGG 8 cut(s) 134, 1862, 2148, 2380, 2388, 2866, 2867, 2965
BstSFI CTRYAG 5 cut(s) 766, 1944, 1976, 2361, 2440
BstSLI GKGCMC 1 cut(s) 2973
BstSNI TACGTA 3 cut(s) 806, 2171, 2356
BstUI CGCG 1 cut(s) 2514
BstV1I GCAGC 4 cut(s) 231, 1694, 2469, 3054
BstV2I GAAGAC 1 cut(s) 944
BstX2I RGATCY 6 cut(s) 166, 823, 917, 1194, 1970, 2392
BstYI RGATCY 6 cut(s) 166, 823, 917, 1194, 1970, 2392
BsuI GTATCC 4 cut(s) 218, 795, 2048, 2589
BsuRI GGCC 6 cut(s) 561, 1456, 2294, 2346, 2615, 2650
BtgZI GCGATG 1 cut(s) 2448
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 5 cut(s) 103, 1264, 1473, 2668, 3031
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 5 cut(s) 117, 562, 1260, 2742, 2833
BtuMI TCGCGA 1 cut(s) 2514
BveI ACCTGC 1 cut(s) 1241
Cac8I GCNNGC 2 cut(s) 1147, 1512
CaiI CAGNNNCTG 1 cut(s) 765
CfoI GCGC 3 cut(s) 283, 672, 3042
Cfr10I RCCGGY 1 cut(s) 2646
Cfr9I CCCGGG 1 cut(s) 2867
Csp6I GTAC 1 cut(s) 2109
CviQI GTAC 1 cut(s) 2109
DpnI GATC 9 cut(s) 168, 792, 825, 919, 1050, 1196, 1972, 2394, 2820
DpnII GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
DriI GACNNNNNGTC 1 cut(s) 2902
EaeI YGGCCR 3 cut(s) 559, 2292, 2648
Eam1104I CTCTTC 1 cut(s) 1377
Eam1105I GACNNNNNGTC 1 cut(s) 2902
EarI CTCTTC 1 cut(s) 1377
Ecl136II GAGCTC 3 cut(s) 433, 2467, 2677
Eco105I TACGTA 3 cut(s) 806, 2171, 2356
Eco130I CCWWGG 2 cut(s) 2446, 3021
Eco24I GRGCYC 3 cut(s) 435, 2469, 2679
Eco47I GGWCC 7 cut(s) 186, 848, 1361, 1589, 1622, 1698, 2782
Eco53kI GAGCTC 3 cut(s) 433, 2467, 2677
Eco57I CTGAAG 6 cut(s) 111, 538, 938, 948, 1200, 2140
Eco88I CYCGRG 3 cut(s) 1342, 2678, 2867
EcoICRI GAGCTC 3 cut(s) 433, 2467, 2677
EcoNI CCTNNNNNAGG 1 cut(s) 1241
EcoO109I RGGNCCY 3 cut(s) 1454, 1622, 2782
EcoRI GAATTC 4 cut(s) 647, 1991, 2093, 2196
EcoRII CCWGG 4 cut(s) 1862, 2148, 2388, 2965
EcoT14I CCWWGG 2 cut(s) 2446, 3021
EcoT22I ATGCAT 2 cut(s) 103, 1516
EcoT38I GRGCYC 3 cut(s) 435, 2469, 2679
ErhI CCWWGG 2 cut(s) 2446, 3021
FalI AAGNNNNNCTT 2 cut(s) 2184, 2216
FaqI GGGAC 9 cut(s) 170, 229, 358, 980, 1635, 1711, 2353, 2795, 2974
FauNDI CATATG 2 cut(s) 537, 1594
Fnu4HI GCNGC 4 cut(s) 245, 1683, 2483, 3043
FokI GGATG 5 cut(s) 110, 1271, 1480, 2675, 3038
FriOI GRGCYC 3 cut(s) 435, 2469, 2679
Fsp4HI GCNGC 4 cut(s) 245, 1683, 2483, 3043
FspBI CTAG 7 cut(s) 59, 821, 1035, 1118, 1146, 1856, 2315
FspI TGCGCA 1 cut(s) 671
GlaI GCGC 3 cut(s) 282, 671, 3041
GluI GCNGC 4 cut(s) 245, 1683, 2483, 3043
GsaI CCCAGC 1 cut(s) 1622
GsuI CTGGAG 3 cut(s) 1080, 2483, 3030
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 6 cut(s) 561, 1456, 2294, 2346, 2615, 2650
HapII CCGG 9 cut(s) 135, 189, 204, 799, 1054, 2381, 2647, 2702, 2868
HhaI GCGC 3 cut(s) 283, 672, 3042
Hin6I GCGC 3 cut(s) 281, 670, 3040
HinP1I GCGC 3 cut(s) 281, 670, 3040
HincII GTYRAC 3 cut(s) 469, 511, 2421
HindII GTYRAC 3 cut(s) 469, 511, 2421
HpaI GTTAAC 2 cut(s) 511, 2421
HpaII CCGG 9 cut(s) 135, 189, 204, 799, 1054, 2381, 2647, 2702, 2868
HphI GGTGA 7 cut(s) 218, 1099, 1302, 1994, 2530, 2680, 2715
Hpy166II GTNNAC 6 cut(s) 469, 511, 1463, 1746, 1836, 2421
Hpy8I GTNNAC 6 cut(s) 469, 511, 1463, 1746, 1836, 2421
HpyCH4III ACNGT 4 cut(s) 989, 1732, 2060, 2365
HpyCH4IV ACGT 9 cut(s) 374, 805, 1957, 2116, 2170, 2355, 2792, 2805, 2898
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 250, 430, 1338, 1520, 2291
HpySE526I ACGT 9 cut(s) 374, 805, 1957, 2116, 2170, 2355, 2792, 2805, 2898
HspAI GCGC 3 cut(s) 281, 670, 3040
Kpn2I TCCGGA 1 cut(s) 1053
KpnI GGTACC 1 cut(s) 2112
KspAI GTTAAC 2 cut(s) 511, 2421
Kzo9I GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
LmnI GCTCC 4 cut(s) 779, 2074, 2464, 2674
Lsp1109I GCAGC 4 cut(s) 231, 1694, 2469, 3054
LweI GCATC 7 cut(s) 88, 1316, 1458, 1557, 1671, 1742, 1889
MaeI CTAG 7 cut(s) 59, 821, 1035, 1118, 1146, 1856, 2315
MaeII ACGT 9 cut(s) 374, 805, 1957, 2116, 2170, 2355, 2792, 2805, 2898
MaeIII GTNAC 5 cut(s) 76, 806, 1105, 2117, 3016
MalI GATC 9 cut(s) 168, 792, 825, 919, 1050, 1196, 1972, 2394, 2820
MboI GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 6 cut(s) 166, 823, 917, 1194, 1970, 2392
MhlI GDGCHC 4 cut(s) 435, 2469, 2679, 2973
MlsI TGGCCA 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 3 cut(s) 2264, 2298, 2912
MmeI TCCRAC 4 cut(s) 678, 2298, 2640, 2826
Mox20I TGGCCA 1 cut(s) 561
Mph1103I ATGCAT 2 cut(s) 103, 1516
MroI TCCGGA 1 cut(s) 1053
MroXI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2572, 2592
MscI TGGCCA 1 cut(s) 561
MslI CAYNNNNRTG 2 cut(s) 1509, 2587
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 2 cut(s) 1685, 1943
MspCI CTTAAG 1 cut(s) 337
MspI CCGG 9 cut(s) 135, 189, 204, 799, 1054, 2381, 2647, 2702, 2868
MspR9I CCNGG 8 cut(s) 136, 1864, 2150, 2382, 2390, 2868, 2869, 2967
MunI CAATTG 1 cut(s) 888
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 4 cut(s) 1864, 2150, 2390, 2967
MvnI CGCG 1 cut(s) 2514
MwoI GCNNNNNNNGC 6 cut(s) 107, 250, 430, 1338, 1520, 2291
NciI CCSGG 4 cut(s) 136, 2382, 2868, 2869
NdeI CATATG 2 cut(s) 537, 1594
NdeII GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
NheI GCTAGC 1 cut(s) 1145
NmuCI GTSAC 3 cut(s) 1105, 2117, 3016
NruI TCGCGA 1 cut(s) 2514
NsbI TGCGCA 1 cut(s) 671
NsiI ATGCAT 2 cut(s) 103, 1516
NspI RCATGY 2 cut(s) 236, 1514
NspV TTCGAA 1 cut(s) 3004
PaeI GCATGC 1 cut(s) 1514
PaeR7I CTCGAG 1 cut(s) 2678
PctI GAATGC 1 cut(s) 1147
PdmI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2572, 2592
PfeI GAWTC 7 cut(s) 131, 262, 712, 835, 1090, 1205, 1576
PflMI CCANNNNNTGG 1 cut(s) 863
PfoI TCCNGGA 1 cut(s) 134
PkrI GCNGC 4 cut(s) 246, 1684, 2484, 3044
PleI GAGTC 3 cut(s) 2264, 2298, 2911
PpsI GAGTC 3 cut(s) 2264, 2298, 2911
Ppu21I YACGTR 4 cut(s) 806, 2117, 2171, 2356
PpuMI RGGWCCY 2 cut(s) 1622, 2782
PsiI TTATAA 2 cut(s) 1691, 1917
Psp124BI GAGCTC 3 cut(s) 435, 2469, 2679
Psp5II RGGWCCY 2 cut(s) 1622, 2782
Psp6I CCWGG 4 cut(s) 1862, 2148, 2388, 2965
PspFI CCCAGC 1 cut(s) 1618
PspGI CCWGG 4 cut(s) 1862, 2148, 2388, 2965
PspPPI RGGWCCY 2 cut(s) 1622, 2782
PstI CTGCAG 1 cut(s) 770
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 6 cut(s) 166, 823, 917, 1194, 1970, 2392
PvuII CAGCTG 2 cut(s) 1685, 1943
RruI TCGCGA 1 cut(s) 2514
RsaI GTAC 1 cut(s) 2110
RsaNI GTAC 1 cut(s) 2109
RseI CAYNNNNRTG 2 cut(s) 1509, 2587
SacI GAGCTC 3 cut(s) 435, 2469, 2679
SatI GCNGC 4 cut(s) 245, 1683, 2483, 3043
Sau3AI GATC 9 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2392, 2818
SchI GAGTC 3 cut(s) 2264, 2298, 2912
ScrFI CCNGG 8 cut(s) 136, 1864, 2150, 2382, 2390, 2868, 2869, 2967
SduI GDGCHC 4 cut(s) 435, 2469, 2679, 2973
SfaNI GCATC 7 cut(s) 88, 1316, 1458, 1557, 1671, 1742, 1889
SfcI CTRYAG 5 cut(s) 766, 1944, 1976, 2361, 2440
Sfr274I CTCGAG 1 cut(s) 2678
SfuI TTCGAA 1 cut(s) 3004
SinI GGWCC 7 cut(s) 186, 848, 1361, 1589, 1622, 1698, 2782
SlaI CTCGAG 1 cut(s) 2678
SmaI CCCGGG 1 cut(s) 2869
SmiMI CAYNNNNRTG 2 cut(s) 1509, 2587
SmlI CTYRAG 3 cut(s) 337, 1111, 2678
SmoI CTYRAG 3 cut(s) 337, 1111, 2678
SnaBI TACGTA 3 cut(s) 806, 2171, 2356
SphI GCATGC 1 cut(s) 1514
SspI AATATT 1 cut(s) 2402
SspMI CTAG 7 cut(s) 59, 821, 1035, 1118, 1146, 1856, 2315
SstI GAGCTC 3 cut(s) 435, 2469, 2679
StyD4I CCNGG 8 cut(s) 134, 1862, 2148, 2380, 2388, 2866, 2867, 2965
StyI CCWWGG 2 cut(s) 2446, 3021
TaaI ACNGT 4 cut(s) 989, 1732, 2060, 2365
TaiI ACGT 9 cut(s) 377, 808, 1960, 2119, 2173, 2358, 2795, 2808, 2901
TaqI TCGA 6 cut(s) 684, 1605, 2032, 2679, 2768, 3004
TaqII GACCGA 2 cut(s) 865, 2015
TfiI GAWTC 7 cut(s) 131, 262, 712, 835, 1090, 1205, 1576
TscAI CASTG 5 cut(s) 117, 562, 1260, 2749, 2833
TseFI GTSAC 3 cut(s) 1105, 2117, 3016
TseI GCWGC 4 cut(s) 244, 1682, 2482, 3042
Tsp45I GTSAC 3 cut(s) 1105, 2117, 3016
TspGWI ACGGA 2 cut(s) 930, 1480
TspMI CCCGGG 1 cut(s) 2867
TspRI CASTG 5 cut(s) 117, 562, 1260, 2749, 2833
Van91I CCANNNNNTGG 1 cut(s) 863
Vha464I CTTAAG 1 cut(s) 337
VpaK11BI GGWCC 7 cut(s) 186, 848, 1361, 1589, 1622, 1698, 2782
XagI CCTNNNNNAGG 1 cut(s) 1241
XapI RAATTY 6 cut(s) 647, 1213, 1565, 1991, 2093, 2196
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 2 cut(s) 236, 1514
XhoI CTCGAG 1 cut(s) 2678
XmaI CCCGGG 1 cut(s) 2867
XmnI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2572, 2592
XspI CTAG 7 cut(s) 59, 821, 1035, 1118, 1146, 1856, 2315
Zsp2I ATGCAT 2 cut(s) 103, 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.