FvH4_6g09470
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002297
Physical Location & Seq
Reverse (-)
5646896 .. 5654334
7439 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09470.t2

Sequence Viewer

Length: 2859 bp
ATGTTGAGGTTGAAATTTTGGGGTTGTCTCATTGCTCTGGCTTGTTCTTGGTTCTTCCTAGTTGCAGTGGCACAAGTTACAAATCCTTCTGAAGTTGATGCATTGAGGGCAGTGAAGGGTAGTTTGATTGATCCCATGAAGCATTTGAGGAATTGGGACAAAGGGGATCCATGTATAGCGAATTGGACCGGAGTTTTGTGTTTCGGTGATGTTGGGACTGATGGATACTTGCATGTCCGGGAGCTGCAACTGCTGAGTATGAATCTCTCAGGAAGCTTAGCACCAGAGCTTGGCAAACTATCTCAACTTCTGATATTAGATTTCATGTGGAATGACTTAAGTGGGACTATACCGAAGGAGATAGGAAACATTATGTCCTTGAAACTCTTGCTCTTGAATGGAAACAGACTATCAGGTTCCTTGCCTGATGAGCTTGGTTATCTTGAAAATCTAAATAGACTACAAGTTGACGAGAACCATCTTTCTGGTCCAGTACCAAAATCATTTGTTAACTTGCGCAGCTTAAAACACCTCCACTTGAATAACAACTCCTTCAGTGGCCAAATTCCATCTGAGATTTCTGGAATACCCACTCTTCTTCATGTGCTCTTGGATAATAATAACTTATCTGGATATCTTCCACCAGAATTCTCCAACATTTCAGGGTTGCGCATACTTCAACTCGATAACAACAACTTCATGGGAACTGAGATTCCAGCTACCTATGGAAACCTTTCCGGATTGGCAAAGTTAAGTCTCAGAAACTGCAGTTTGCAGGGAACAATTCCTGATCTTAGCCAGATACCTAACCTCAGTTATCTAGATCTAAGCTGGAATCAGCTTGCTGGACCAATAGCATCACATAGACTTTCTGACAATATGACAACAATTGATCTGTCAGACAACCATCTTAACGGATCTATTCCTGAAAGTTTCTCCAATCTTCCTCATCTTCAGAAATTGTCGCTCGAGAATAATTTGTTGAATGGTTCTGTCCCTGCTGTATGGAGGAACATATCTTTTAGTTCAAGAGCTAGACTTTCATTTGATCTCCGGAATAATTCACTCTCTAACATATTAGGAGAGCTGAATCCTCCAGCAAATGTCACCTTGAGGCTTGAAGGAAATCCTATTTGCAAGAATGCTAGCATAGAAAATATAATCCCGTTCTGTCAATCTGAAGTGAGAGATGGCATCCCTGAAGATTCAATAAATTCGACTCAACAGATGAACTGCCCTATTCAAGCATGTCCAGTGGATGATTTCTATGAATATGTTCCATCTTCCCCGGTGCCATGCTATTGTGCATCACCTATTATAATTGAATATCGTCTCAAAAGCCCAAGCTTTTCTTATTTTCCTCCATATATACAGCAGTTTGAAGAATATTTAACTAGTTATCTCAAATTGGAGATTTATCAGTTATCAATAAGTTCCTTTGTGTGGGAAAAGGGTCATCGCTTACGGATGCAGTTGAAGTTCTTCCCTGTGTATGGTGCTTCACACTCATATACATTTAACGAAAGTGAGGTTCTGCGGATTAGAGGCATCTTTACAGCATGGCTATTTCCTCAAAATGATTTCTTTGGACCTTATGAGCTACTCAAGTTCAATCTGCTGGGACCTTATTCAAATATGATTGTTAAGAGACAAAGGATGGTCATCAGTAAGGGTGTATTAGCAGCTGTTATAATAGGGGCCATTGCTTCGGTTGTCAGTGTATCTGCAACAGTCATGCTATTGTTCACAAGATGCCGCAGAAACAGATACCCACCACCTTCAAGGAGACACTCATCGTCGAAGATTTCTATGAGAATTGAAGGTGTAAAGTCTTTCACTTTCAAAGAAATGACGCTAGCCACCAGGAACTTTGATAGTTCAACTCAACTTGGACGAGGAGGTTATGGGAAAGTTTACAAAGGTATCTTATCTGATAACACAATTGTAGCCATAAAACGTGCAGAAGAAGGATCCCTACAGGGTGAAAAAGAATTCTTGACTGAGATAGAATTGTTATCAAGGCTACACCATCGAAACTTAGTCTCGTTGGTAGGATACTGTGAAGAAGAAGAGGAGCAGATGTTGGTTTATGAGTTCATGCCTAATGGTACCTTACGTGACTGGCTTTCTGATAAAATCAAGGGAAGCCTGGACTTCAGTATGAGGTTACGTATTGCATTGGGTTCAGCCAAAGGAATTCTTTATCTCCATACCGAGGCGAATCCTCCTATATTTCACCGAGATATTAAAGCCACCAACATACTTTTGGACTCCAACTATACTGCTAAGGTGGCTGATTTTGGACTCTCACGACTTGCTCCTGTCCAGGATGATGAAGGGACTGGCCCTGGATACGTATCTACAGTTGTGAGAGGAACACCGGGCTACCTGGATCCAGAGTATTTCTTGACCAGTAAGTTAACAGACAAAAGCGATGTGTATAGCCTTGGAATTGTGTTTCTGGAGCTCTTGACTGGTGTGCTGCCAATATCACATGGCAAAAACATTGTTCGGGAGGTGAATATGGCTCATCAGTCGGGCTTAATGTTCTCCATTATAGATACCAGAATGGGTTCATATCCATCTGAATGTGTCGAGAGGTTTGTAGCTTTGGCCCTCAGATGTTGCCAAGAAAAACAAGACGACCGGCCATCAATGTTAGATGTGGTGAGGGAGCTTGAAAACATCATCAAAATTATGCCGGGAGCTGATACAATATTCTCACCATCTGGTTCCTCATACAATGAAAAATCACCAACTTCTTCTTCGACTTTTACTGGGGACCCGTATATGTGCTCCTCCAGTGTACAAGGAAGTGATCTAAACAGTGGTGTGGTCCCTACTATCTTGCCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

953

Amino Acids

106.03

Weight (kDa)

6.11

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 50 - 88 1.9e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 110 - 202 3.8e-08 Leucine-rich repeat region
LRR_8 PF13855 171 - 230 2.3e-06 Leucine rich repeat
LRR_14 PF23598 190 - 348 2.1e-08 Leucine-rich repeat region
LRR_8 PF13855 314 - 349 5.8e-06 Leucine rich repeat
Pkinase PF00069 647 - 912 2.5e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 648 - 914 1.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1319, 1691
Acc16I TGCGCA 2 cut(s) 518, 671
Acc65I GGTACC 1 cut(s) 2108
AccB1I GGYRCC 2 cut(s) 1291, 2108
AccB7I CCANNNNNTGG 1 cut(s) 290
AccIII TCCGGA 2 cut(s) 737, 1053
AciI CCGC 2 cut(s) 1537, 1756
AclWI GGATC 8 cut(s) 125, 161, 174, 925, 1965, 1978, 2387, 2400
AcoI YGGCCR 2 cut(s) 559, 2648
AcsI RAATTY 6 cut(s) 14, 564, 647, 1213, 1991, 2196
AcuI CTGAAG 6 cut(s) 111, 538, 938, 1200, 1221, 2140
AfaI GTAC 3 cut(s) 495, 2110, 2808
AfiI CCNNNNNNNGG 4 cut(s) 290, 1443, 1493, 2215
AflII CTTAAG 1 cut(s) 337
AhlI ACTAGT 1 cut(s) 1394
AjnI CCWGG 5 cut(s) 1862, 2148, 2325, 2347, 2388
AjuI GAANNNNNNNTTGG 2 cut(s) 2749, 2781
AloI GAACNNNNNNTCC 2 cut(s) 697, 729
Alw21I GWGCWC 3 cut(s) 609, 2469, 2798
Alw26I GTCTC 6 cut(s) 32, 761, 1337, 1642, 1780, 2047
AlwI GGATC 8 cut(s) 125, 161, 174, 925, 1965, 1978, 2387, 2400
AlwNI CAGNNNCTG 1 cut(s) 765
Ama87I CYCGRG 1 cut(s) 968
Aor13HI TCCGGA 2 cut(s) 737, 1053
AoxI GGCC 5 cut(s) 559, 1698, 2344, 2613, 2648
ApeKI GCWGC 4 cut(s) 244, 519, 1682, 2482
ApoI RAATTY 6 cut(s) 14, 564, 647, 1213, 1991, 2196
ArsI GACNNNNNNTTYG 2 cut(s) 178, 210
Asp700I GAANNNNTTC 4 cut(s) 733, 1275, 1481, 2572
Asp718I GGTACC 1 cut(s) 2108
AspLEI GCGC 2 cut(s) 519, 672
AsuC2I CCSGG 4 cut(s) 239, 1289, 2382, 2703
AsuHPI GGTGA 9 cut(s) 218, 1099, 1302, 1994, 2228, 2530, 2680, 2715, 2745
AsuNHI GCTAGC 2 cut(s) 1145, 1855
AvaI CYCGRG 1 cut(s) 968
AvaII GGWCC 7 cut(s) 186, 488, 848, 1589, 1622, 2782, 2836
BaeI ACNNNNGTAYC 2 cut(s) 1906, 1939
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 3 cut(s) 166, 1970, 2392
BanI GGYRCC 2 cut(s) 1291, 2108
BanII GRGCYC 1 cut(s) 2469
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
Bbv12I GWGCWC 3 cut(s) 609, 2469, 2798
BbvI GCAGC 4 cut(s) 231, 531, 1694, 2469
BciT130I CCWGG 5 cut(s) 1864, 2150, 2327, 2349, 2390
BciVI GTATCC 3 cut(s) 218, 2048, 2345
BcnI CCSGG 4 cut(s) 239, 1289, 2382, 2703
BcoDI GTCTC 6 cut(s) 32, 761, 1337, 1642, 1780, 2047
BcuI ACTAGT 1 cut(s) 1394
BfaI CTAG 6 cut(s) 59, 821, 1035, 1146, 1395, 1856
BfmI CTRYAG 3 cut(s) 766, 1976, 2361
BfrI CTTAAG 1 cut(s) 337
BfuI GTATCC 3 cut(s) 218, 2048, 2345
BglII AGATCT 1 cut(s) 823
BisI GCNGC 5 cut(s) 245, 520, 1683, 1756, 2483
BlpI GCTNAGC 1 cut(s) 277
BlsI GCNGC 5 cut(s) 246, 521, 1684, 1757, 2484
Bme1390I CCNGG 9 cut(s) 239, 1289, 1864, 2150, 2327, 2349, 2382, 2390, 2703
Bme18I GGWCC 7 cut(s) 186, 488, 848, 1589, 1622, 2782, 2836
BmeT110I CYCGRG 1 cut(s) 968
BmrFI CCNGG 9 cut(s) 239, 1289, 1864, 2150, 2327, 2349, 2382, 2390, 2703
BmrI ACTGGG 1 cut(s) 2787
BmsI GCATC 7 cut(s) 88, 866, 1203, 1316, 1458, 1557, 1742
BmtI GCTAGC 2 cut(s) 1149, 1859
BmuI ACTGGG 1 cut(s) 2787
BpmI CTGGAG 3 cut(s) 1080, 2483, 2785
Bpu10I CCTNAGC 1 cut(s) 2286
Bpu1102I GCTNAGC 1 cut(s) 277
BpuEI CTTGAG 2 cut(s) 1132, 1589
BpuMI CCSGG 4 cut(s) 239, 1289, 2382, 2703
BsaAI YACGTR 3 cut(s) 2117, 2171, 2356
BsaBI GATNNNNATC 2 cut(s) 1661, 2356
BsaJI CCNNGG 4 cut(s) 1287, 2214, 2347, 2446
BsaWI WCCGGW 3 cut(s) 188, 737, 1053
BsaXI ACNNNNNCTCC 2 cut(s) 2066, 2096
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 1443, 1493, 2215
Bse118I RCCGGY 1 cut(s) 2646
Bse1I ACTGG 8 cut(s) 491, 1253, 2126, 2347, 2412, 2479, 2782, 2802
Bse3DI GCAATG 2 cut(s) 30, 1701
Bse8I GATNNNNATC 2 cut(s) 1661, 2356
BseAI TCCGGA 2 cut(s) 737, 1053
BseBI CCWGG 5 cut(s) 1864, 2150, 2327, 2349, 2390
BseDI CCNNGG 4 cut(s) 1287, 2214, 2347, 2446
BseGI GGATG 5 cut(s) 1194, 1264, 1473, 1662, 2335
BseJI GATNNNNATC 2 cut(s) 1661, 2356
BseLI CCNNNNNNNGG 4 cut(s) 290, 1443, 1493, 2215
BseMI GCAATG 2 cut(s) 30, 1701
BseMII CTCAG 8 cut(s) 245, 282, 564, 699, 772, 826, 1992, 2632
BseNI ACTGG 8 cut(s) 491, 1253, 2126, 2347, 2412, 2479, 2782, 2802
BseRI GAGGAG 3 cut(s) 1911, 2087, 2788
BseXI GCAGC 4 cut(s) 231, 531, 1694, 2469
BseYI CCCAGC 1 cut(s) 1618
BsgI GTGCAG 1 cut(s) 1980
Bsh1285I CGRYCG 1 cut(s) 2647
BshFI GGCC 5 cut(s) 561, 1700, 2346, 2615, 2650
BshNI GGYRCC 2 cut(s) 1291, 2108
BsiEI CGRYCG 1 cut(s) 2647
BsiHKAI GWGCWC 3 cut(s) 609, 2469, 2798
BsiHKCI CYCGRG 1 cut(s) 968
BsiSI CCGG 8 cut(s) 189, 238, 738, 1054, 1289, 2381, 2647, 2702
BslFI GGGAC 8 cut(s) 170, 229, 358, 980, 1635, 2353, 2795, 2822
BslI CCNNNNNNNGG 4 cut(s) 290, 1443, 1493, 2215
BsmAI GTCTC 6 cut(s) 32, 761, 1337, 1642, 1780, 2047
BsmBI CGTCTC 1 cut(s) 1337
BsmFI GGGAC 8 cut(s) 170, 229, 358, 980, 1635, 2353, 2795, 2822
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 5 cut(s) 561, 1700, 2346, 2615, 2650
BsoBI CYCGRG 1 cut(s) 968
Bsp1286I GDGCHC 3 cut(s) 609, 2469, 2798
Bsp13I TCCGGA 2 cut(s) 737, 1053
Bsp1407I TGTACA 1 cut(s) 2806
Bsp1720I GCTNAGC 1 cut(s) 277
BspACI CCGC 2 cut(s) 1537, 1756
BspANI GGCC 5 cut(s) 561, 1700, 2346, 2615, 2650
BspCNI CTCAG 8 cut(s) 246, 281, 565, 700, 771, 825, 1993, 2631
BspEI TCCGGA 2 cut(s) 737, 1053
BspMAI CTGCAG 1 cut(s) 770
BspOI GCTAGC 2 cut(s) 1149, 1859
BspPI GGATC 8 cut(s) 125, 161, 174, 925, 1965, 1978, 2387, 2400
BspT107I GGYRCC 2 cut(s) 1291, 2108
BspTI CTTAAG 1 cut(s) 337
BsrDI GCAATG 2 cut(s) 30, 1701
BsrFI RCCGGY 1 cut(s) 2646
BsrGI TGTACA 1 cut(s) 2806
BsrI ACTGG 8 cut(s) 491, 1253, 2126, 2347, 2412, 2479, 2782, 2802
BssAI RCCGGY 1 cut(s) 2646
BssECI CCNNGG 4 cut(s) 1287, 2214, 2347, 2446
BssT1I CCWWGG 1 cut(s) 2446
Bst2UI CCWGG 5 cut(s) 1864, 2150, 2327, 2349, 2390
Bst4CI ACNGT 4 cut(s) 1732, 2060, 2365, 2828
Bst6I CTCTTC 2 cut(s) 600, 2064
BstAFI CTTAAG 1 cut(s) 337
BstAUI TGTACA 1 cut(s) 2806
BstBAI YACGTR 3 cut(s) 2117, 2171, 2356
BstC8I GCNNGC 3 cut(s) 843, 1147, 1857
BstF5I GGATG 5 cut(s) 1194, 1264, 1473, 1662, 2335
BstHHI GCGC 2 cut(s) 519, 672
BstMAI GTCTC 6 cut(s) 32, 761, 1337, 1642, 1780, 2047
BstMCI CGRYCG 1 cut(s) 2647
BstMWI GCNNNNNNNGC 4 cut(s) 107, 250, 430, 2291
BstNI CCWGG 5 cut(s) 1864, 2150, 2327, 2349, 2390
BstNSI RCATGY 2 cut(s) 236, 1251
BstSCI CCNGG 9 cut(s) 237, 1287, 1862, 2148, 2325, 2347, 2380, 2388, 2701
BstSFI CTRYAG 3 cut(s) 766, 1976, 2361
BstSNI TACGTA 2 cut(s) 2171, 2356
BstV1I GCAGC 4 cut(s) 231, 531, 1694, 2469
BstX2I RGATCY 5 cut(s) 166, 823, 917, 1970, 2392
BstXI CCANNNNNNTGG 1 cut(s) 485
BstYI RGATCY 5 cut(s) 166, 823, 917, 1970, 2392
BsuI GTATCC 3 cut(s) 218, 2048, 2345
BsuRI GGCC 5 cut(s) 561, 1700, 2346, 2615, 2650
BtgZI GCGATG 2 cut(s) 1442, 2448
BtsCI GGATG 5 cut(s) 1194, 1264, 1473, 1662, 2335
BtsI GCAGTG 2 cut(s) 72, 117
BtsIMutI CAGTG 7 cut(s) 72, 117, 562, 1260, 1723, 2809, 2833
Cac8I GCNNGC 3 cut(s) 843, 1147, 1857
CaiI CAGNNNCTG 1 cut(s) 765
CfoI GCGC 2 cut(s) 519, 672
Cfr10I RCCGGY 1 cut(s) 2646
CseI GACGC 1 cut(s) 1861
Csp6I GTAC 3 cut(s) 494, 2109, 2807
CviQI GTAC 3 cut(s) 494, 2109, 2807
EaeI YGGCCR 2 cut(s) 559, 2648
Eam1104I CTCTTC 2 cut(s) 600, 2064
EarI CTCTTC 2 cut(s) 600, 2064
Ecl136II GAGCTC 1 cut(s) 2467
Eco105I TACGTA 2 cut(s) 2171, 2356
Eco130I CCWWGG 1 cut(s) 2446
Eco24I GRGCYC 1 cut(s) 2469
Eco32I GATATC 1 cut(s) 635
Eco47I GGWCC 7 cut(s) 186, 488, 848, 1589, 1622, 2782, 2836
Eco53kI GAGCTC 1 cut(s) 2467
Eco57I CTGAAG 6 cut(s) 111, 538, 938, 1200, 1221, 2140
Eco88I CYCGRG 1 cut(s) 968
EcoICRI GAGCTC 1 cut(s) 2467
EcoO109I RGGNCCY 2 cut(s) 1622, 2782
EcoRI GAATTC 3 cut(s) 647, 1991, 2196
EcoRII CCWGG 5 cut(s) 1862, 2148, 2325, 2347, 2388
EcoRV GATATC 1 cut(s) 635
EcoT14I CCWWGG 1 cut(s) 2446
EcoT22I ATGCAT 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 2469
ErhI CCWWGG 1 cut(s) 2446
Esp3I CGTCTC 1 cut(s) 1337
FalI AAGNNNNNCTT 4 cut(s) 1336, 1368, 2184, 2216
FaqI GGGAC 8 cut(s) 170, 229, 358, 980, 1635, 2353, 2795, 2822
Fnu4HI GCNGC 5 cut(s) 245, 520, 1683, 1756, 2483
FokI GGATG 5 cut(s) 1181, 1271, 1480, 1669, 2342
FriOI GRGCYC 1 cut(s) 2469
Fsp4HI GCNGC 5 cut(s) 245, 520, 1683, 1756, 2483
FspBI CTAG 6 cut(s) 59, 821, 1035, 1146, 1395, 1856
FspI TGCGCA 2 cut(s) 518, 671
GlaI GCGC 2 cut(s) 518, 671
GluI GCNGC 5 cut(s) 245, 520, 1683, 1756, 2483
GsaI CCCAGC 1 cut(s) 1622
GsuI CTGGAG 3 cut(s) 1080, 2483, 2785
HaeIII GGCC 5 cut(s) 561, 1700, 2346, 2615, 2650
HapII CCGG 8 cut(s) 189, 238, 738, 1054, 1289, 2381, 2647, 2702
HgaI GACGC 1 cut(s) 1861
HhaI GCGC 2 cut(s) 519, 672
Hin6I GCGC 2 cut(s) 517, 670
HinP1I GCGC 2 cut(s) 517, 670
HincII GTYRAC 3 cut(s) 469, 511, 2421
HindII GTYRAC 3 cut(s) 469, 511, 2421
HindIII AAGCTT 2 cut(s) 274, 1345
HinfI GANTC 9 cut(s) 262, 712, 835, 1090, 1205, 1219, 2221, 2270, 2304
HpaI GTTAAC 2 cut(s) 511, 2421
HpaII CCGG 8 cut(s) 189, 238, 738, 1054, 1289, 2381, 2647, 2702
HphI GGTGA 9 cut(s) 218, 1099, 1302, 1994, 2228, 2530, 2680, 2715, 2745
Hpy166II GTNNAC 6 cut(s) 469, 511, 1746, 1915, 2421, 2807
Hpy8I GTNNAC 6 cut(s) 469, 511, 1746, 1915, 2421, 2807
Hpy99I CGWCG 1 cut(s) 1801
HpyAV CCTTC 9 cut(s) 96, 109, 349, 562, 1115, 1788, 1814, 1961, 2330
HpyCH4III ACNGT 4 cut(s) 1732, 2060, 2365, 2828
HpyCH4IV ACGT 4 cut(s) 1957, 2116, 2170, 2355
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 250, 430, 2291
HpySE526I ACGT 4 cut(s) 1957, 2116, 2170, 2355
HspAI GCGC 2 cut(s) 517, 670
KflI GGGWCCC 1 cut(s) 2782
Kpn2I TCCGGA 2 cut(s) 737, 1053
KpnI GGTACC 1 cut(s) 2112
KspAI GTTAAC 2 cut(s) 511, 2421
LmnI GCTCC 7 cut(s) 241, 2074, 2323, 2464, 2674, 2705, 2801
Lsp1109I GCAGC 4 cut(s) 231, 531, 1694, 2469
LweI GCATC 7 cut(s) 88, 866, 1203, 1316, 1458, 1557, 1742
MaeI CTAG 6 cut(s) 59, 821, 1035, 1146, 1395, 1856
MaeII ACGT 4 cut(s) 1957, 2116, 2170, 2355
MaeIII GTNAC 4 cut(s) 76, 1105, 2117, 2166
MfeI CAATTG 2 cut(s) 888, 1941
MflI RGATCY 5 cut(s) 166, 823, 917, 1970, 2392
MhlI GDGCHC 3 cut(s) 609, 2469, 2798
MlsI TGGCCA 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 3 cut(s) 1213, 2264, 2298
MmeI TCCRAC 2 cut(s) 678, 2298
Mox20I TGGCCA 1 cut(s) 561
Mph1103I ATGCAT 1 cut(s) 103
MroI TCCGGA 2 cut(s) 737, 1053
MroXI GAANNNNTTC 4 cut(s) 733, 1275, 1481, 2572
MscI TGGCCA 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 2587
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 1 cut(s) 1685
MspCI CTTAAG 1 cut(s) 337
MspI CCGG 8 cut(s) 189, 238, 738, 1054, 1289, 2381, 2647, 2702
MspR9I CCNGG 9 cut(s) 239, 1289, 1864, 2150, 2327, 2349, 2382, 2390, 2703
MunI CAATTG 2 cut(s) 888, 1941
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 5 cut(s) 1864, 2150, 2327, 2349, 2390
MwoI GCNNNNNNNGC 4 cut(s) 107, 250, 430, 2291
NciI CCSGG 4 cut(s) 239, 1289, 2382, 2703
NheI GCTAGC 2 cut(s) 1145, 1855
NmuCI GTSAC 2 cut(s) 1105, 2117
NsbI TGCGCA 2 cut(s) 518, 671
NsiI ATGCAT 1 cut(s) 103
NspI RCATGY 2 cut(s) 236, 1251
PaeR7I CTCGAG 1 cut(s) 968
PctI GAATGC 1 cut(s) 1147
PdmI GAANNNNTTC 4 cut(s) 733, 1275, 1481, 2572
PfeI GAWTC 6 cut(s) 262, 712, 835, 1090, 1205, 2221
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 2 cut(s) 237, 2325
PkrI GCNGC 5 cut(s) 246, 521, 1684, 1757, 2484
PleI GAGTC 3 cut(s) 1213, 2264, 2298
PpsI GAGTC 3 cut(s) 1213, 2264, 2298
Ppu21I YACGTR 3 cut(s) 2117, 2171, 2356
PpuMI RGGWCCY 2 cut(s) 1622, 2782
PsiI TTATAA 2 cut(s) 1319, 1691
Psp124BI GAGCTC 1 cut(s) 2469
Psp5II RGGWCCY 2 cut(s) 1622, 2782
Psp6I CCWGG 5 cut(s) 1862, 2148, 2325, 2347, 2388
PspFI CCCAGC 1 cut(s) 1618
PspGI CCWGG 5 cut(s) 1862, 2148, 2325, 2347, 2388
PspPPI RGGWCCY 2 cut(s) 1622, 2782
PstI CTGCAG 1 cut(s) 770
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 5 cut(s) 166, 823, 917, 1970, 2392
PvuII CAGCTG 1 cut(s) 1685
RsaI GTAC 3 cut(s) 495, 2110, 2808
RsaNI GTAC 3 cut(s) 494, 2109, 2807
RseI CAYNNNNRTG 1 cut(s) 2587
SacI GAGCTC 1 cut(s) 2469
SatI GCNGC 5 cut(s) 245, 520, 1683, 1756, 2483
SchI GAGTC 3 cut(s) 1213, 2264, 2298
ScrFI CCNGG 9 cut(s) 239, 1289, 1864, 2150, 2327, 2349, 2382, 2390, 2703
SduI GDGCHC 3 cut(s) 609, 2469, 2798
SfaNI GCATC 7 cut(s) 88, 866, 1203, 1316, 1458, 1557, 1742
SfcI CTRYAG 3 cut(s) 766, 1976, 2361
Sfr274I CTCGAG 1 cut(s) 968
SinI GGWCC 7 cut(s) 186, 488, 848, 1589, 1622, 2782, 2836
SlaI CTCGAG 1 cut(s) 968
SmiMI CAYNNNNRTG 1 cut(s) 2587
SmlI CTYRAG 4 cut(s) 337, 968, 1111, 1604
SmoI CTYRAG 4 cut(s) 337, 968, 1111, 1604
SnaBI TACGTA 2 cut(s) 2171, 2356
SpeI ACTAGT 1 cut(s) 1394
SsiI CCGC 2 cut(s) 1537, 1756
SspI AATATT 2 cut(s) 1388, 2718
SspMI CTAG 6 cut(s) 59, 821, 1035, 1146, 1395, 1856
SstI GAGCTC 1 cut(s) 2469
StyD4I CCNGG 9 cut(s) 237, 1287, 1862, 2148, 2325, 2347, 2380, 2388, 2701
StyI CCWWGG 1 cut(s) 2446
TaaI ACNGT 4 cut(s) 1732, 2060, 2365, 2828
TaiI ACGT 4 cut(s) 1960, 2119, 2173, 2358
TaqI TCGA 8 cut(s) 684, 969, 1217, 1799, 2032, 2595, 2768, 2854
TatI WGTACW 1 cut(s) 2806
TauI GCSGC 1 cut(s) 1758
TfiI GAWTC 6 cut(s) 262, 712, 835, 1090, 1205, 2221
TscAI CASTG 7 cut(s) 72, 117, 562, 1260, 1723, 2809, 2833
TseFI GTSAC 2 cut(s) 1105, 2117
TseI GCWGC 4 cut(s) 244, 519, 1682, 2482
Tsp45I GTSAC 2 cut(s) 1105, 2117
TspGWI ACGGA 2 cut(s) 930, 1480
TspRI CASTG 7 cut(s) 72, 117, 562, 1260, 1723, 2809, 2833
Van91I CCANNNNNTGG 1 cut(s) 290
Vha464I CTTAAG 1 cut(s) 337
VpaK11BI GGWCC 7 cut(s) 186, 488, 848, 1589, 1622, 2782, 2836
XapI RAATTY 6 cut(s) 14, 564, 647, 1213, 1991, 2196
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 2 cut(s) 236, 1251
XcmI CCANNNNNNNNNTGG 1 cut(s) 2263
XhoI CTCGAG 1 cut(s) 968
XmnI GAANNNNTTC 4 cut(s) 733, 1275, 1481, 2572
XspI CTAG 6 cut(s) 59, 821, 1035, 1146, 1395, 1856
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.