Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002297

Physical Location & Seq

Genomic Coordinates Forward (+)

26873722 .. 26875667

Gene Structure 1946 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1177400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGTCACTCTGCGACCTCATTCAAGGGTTTTGTGGTTGTCAGTATTCTGTTTTGGTGGTGATAGTCTCGGAACTCGTTCTCGTTCATAGACCTAGATCCCGTGGGGATTTTGGACTCTCGCGACTTGCACCTCTGCAGGATGATGCAGGGATCGGGCCTGCTTATGTATCTACAATTGTGAGAGGAACGCCGGGTTACCTTGATCCAGAGTATTTCCTGACCAATAAGTTGACAGACAAAAGCGATGTCTATAGCCTAGGAATCGTGTTTCTGGAGCTCCTTACGGGCAATCAGCCAATTTCACACGGCAAAAACATAGTTCGAGAGGTGAATCTGGCTCATCAGGCAGGGCTGGTGTTCTCGATCATAGACAGCAGAATGGGTTCCTATCCATCTGAATGCGTTGAAAGGCTTCTAGATTTGGCCCTCAGATGTTGTAATGAAAAGCAAGACAAGCGGCCTCATATGCTGGAAGTGGTGAGGGAGCTCGAAAACATACTCAAAATCACGCCGGCAACTGACACCATATTTTCACCACCTACTTCCTCGTACAGTGACCAATCACCAACCTCATCGTCCTTATTGACTGGAGACCGATCCTACGTGTCCTCCAGCCTGGCAGGAAGTGATCTAACCAGTGGTACTGTCCCAAACATAGTGCCTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

223

Amino Acids

24.19

Weight (kDa)

5.35

Isoelectric Point (pI)

45.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	35 - 163	5.9e-12	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	36 - 161	1.6e-14	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002297)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01950 AT5G01950 AT5G01950 AT5G01950 AT5G01950 AT5G01950 AT5G01950 AT5G01950
fragaria_vesca	FvH4_6g09451 FvH4_6g09451 FvH4_6g09470 FvH4_6g09470
malus_domestica	MD04G1177400.v1.1 MD12G1192800.v1.1
prunus_persica	Prupe.6G297000_v2.0.a1 Prupe.6G297000_v2.0.a1 Prupe.6G297000_v2.0.a1 Prupe.6G297100_v2.0.a1 Prupe.6G297200_v2.0.a1 Prupe.6G297400_v2.0.a1
pyrus_communis	pycom12g17950
rosa_chinensis	RchiOBHm_Chr3g0459941 RchiOBHm_Chr3g0459991 RchiOBHm_Chr3g0460021 RchiOBHm_Chr3g0460051
rosa_laevigata	RLG00000025048 RLG00000025050
rosa_multiflora	Rmu_sc0000240.1_g000049 Rmu_sc0000240.1_g000076 Rmu_sc0013658.1_g000003 Rmu_sc0030602.1_g000001
rosa_roxburghii	Rroxscaffold_6G00419890 Rroxscaffold_6G00419900
rosa_rugosa	Rorug03G0038900 Rorug03G0039100
rosa_samantha	Rh3BG100400 Rh3BG100600 Rh3CG102200 Rh3CG102400 Rh3CG102700 Rh3DG101900
rosa_wichuraiana	Rw3G008220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	121
AciI	CCGC	1 cut(s)	457
AclWI	GGATC	4 cut(s)	90, 158, 197, 591
AfaI	GTAC	2 cut(s)	551, 643
AflIII	ACRYGT	1 cut(s)	603
AgsI	TTSAA	2 cut(s)	23, 407
AjnI	CCWGG	1 cut(s)	615
AluBI	AGCT	2 cut(s)	277, 487
AluI	AGCT	2 cut(s)	277, 487
Alw21I	GWGCWC	2 cut(s)	279, 489
Alw26I	GTCTC	2 cut(s)	70, 585
AlwI	GGATC	4 cut(s)	90, 158, 197, 591
AoxI	GGCC	3 cut(s)	155, 423, 458
ArsI	GACNNNNNNTTYG	2 cut(s)	231, 263
Asp700I	GAANNNNTTC	3 cut(s)	75, 382, 411
AspA2I	CCTAGG	1 cut(s)	256
AspS9I	GGNCC	2 cut(s)	155, 424
AsuC2I	CCSGG	1 cut(s)	192
AsuHPI	GGTGA	5 cut(s)	70, 340, 490, 525, 555
AvrII	CCTAGG	1 cut(s)	256
BanII	GRGCYC	2 cut(s)	279, 489
Bbv12I	GWGCWC	2 cut(s)	279, 489
BccI	CCATC	1 cut(s)	400
BceAI	ACGGC	1 cut(s)	322
BciT130I	CCWGG	1 cut(s)	617
BcnI	CCSGG	1 cut(s)	192
BcoDI	GTCTC	2 cut(s)	70, 585
BfaI	CTAG	3 cut(s)	93, 257, 416
BfmI	CTRYAG	2 cut(s)	134, 250
BisI	GCNGC	1 cut(s)	458
BlnI	CCTAGG	1 cut(s)	256
BlsI	GCNGC	1 cut(s)	459
Bme1390I	CCNGG	2 cut(s)	192, 617
BmgT120I	GGNCC	2 cut(s)	155, 424
BmiI	GGNNCC	1 cut(s)	385
BmrFI	CCNGG	2 cut(s)	192, 617
BmsI	GCATC	1 cut(s)	133
BpmI	CTGGAG	3 cut(s)	293, 595, 609
BpuMI	CCSGG	1 cut(s)	192
BsaAI	YACGTR	1 cut(s)	604
BsaI	GGTCTC	1 cut(s)	585
BsaJI	CCNNGG	2 cut(s)	100, 256
BsaXI	ACNNNNNCTCC	2 cut(s)	593, 623
Bse118I	RCCGGY	1 cut(s)	511
Bse1I	ACTGG	2 cut(s)	592, 636
BseBI	CCWGG	1 cut(s)	617
BseDI	CCNNGG	2 cut(s)	100, 256
BseGI	GGATG	1 cut(s)	145
BseMII	CTCAG	1 cut(s)	442
BseNI	ACTGG	2 cut(s)	592, 636
Bsh1236I	CGCG	1 cut(s)	121
BshFI	GGCC	3 cut(s)	157, 425, 460
BsiHKAI	GWGCWC	2 cut(s)	279, 489
BsiSI	CCGG	2 cut(s)	191, 512
BslFI	GGGAC	1 cut(s)	632
BsmAI	GTCTC	2 cut(s)	70, 585
BsmFI	GGGAC	1 cut(s)	632
BsmI	GAATGC	1 cut(s)	404
BsnI	GGCC	3 cut(s)	157, 425, 460
Bso31I	GGTCTC	1 cut(s)	585
Bsp1286I	GDGCHC	2 cut(s)	279, 489
Bsp143I	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
Bsp68I	TCGCGA	1 cut(s)	121
BspACI	CCGC	1 cut(s)	457
BspANI	GGCC	3 cut(s)	157, 425, 460
BspCNI	CTCAG	1 cut(s)	441
BspFNI	CGCG	1 cut(s)	121
BspLI	GGNNCC	1 cut(s)	385
BspMAI	CTGCAG	1 cut(s)	138
BspPI	GGATC	4 cut(s)	90, 158, 197, 591
BspTNI	GGTCTC	1 cut(s)	585
BsrFI	RCCGGY	1 cut(s)	511
BsrI	ACTGG	2 cut(s)	592, 636
BssAI	RCCGGY	1 cut(s)	511
BssECI	CCNNGG	2 cut(s)	100, 256
BssMI	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
BssT1I	CCWWGG	1 cut(s)	256
Bst2UI	CCWGG	1 cut(s)	617
Bst4CI	ACNGT	2 cut(s)	554, 646
BstBAI	YACGTR	1 cut(s)	604
BstC8I	GCNNGC	2 cut(s)	159, 513
BstDEI	CTNAG	1 cut(s)	428
BstDSI	CCRYGG	1 cut(s)	100
BstEII	GGTNACC	1 cut(s)	194
BstF5I	GGATG	1 cut(s)	145
BstFNI	CGCG	1 cut(s)	121
BstKTI	GATC	6 cut(s)	98, 153, 205, 366, 599, 631
BstMAI	GTCTC	2 cut(s)	70, 585
BstMBI	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
BstMWI	GCNNNNNNNGC	3 cut(s)	344, 454, 466
BstNI	CCWGG	1 cut(s)	617
BstPI	GGTNACC	1 cut(s)	194
BstSCI	CCNGG	2 cut(s)	190, 615
BstSFI	CTRYAG	2 cut(s)	134, 250
BstUI	CGCG	1 cut(s)	121
BstX2I	RGATCY	1 cut(s)	95
BstYI	RGATCY	1 cut(s)	95
BsuRI	GGCC	3 cut(s)	157, 425, 460
BtgI	CCRYGG	1 cut(s)	100
BtgZI	GCGATG	1 cut(s)	258
BtsCI	GGATG	1 cut(s)	145
BtsIMutI	CAGTG	2 cut(s)	559, 643
BtuMI	TCGCGA	1 cut(s)	121
Cac8I	GCNNGC	2 cut(s)	159, 513
Cfr10I	RCCGGY	1 cut(s)	511
Cfr13I	GGNCC	2 cut(s)	155, 424
Csp6I	GTAC	2 cut(s)	550, 642
CviQI	GTAC	2 cut(s)	550, 642
DdeI	CTNAG	1 cut(s)	428
DpnI	GATC	6 cut(s)	97, 152, 204, 365, 598, 630
DpnII	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
Ecl136II	GAGCTC	2 cut(s)	277, 487
Eco130I	CCWWGG	1 cut(s)	256
Eco24I	GRGCYC	2 cut(s)	279, 489
Eco31I	GGTCTC	1 cut(s)	585
Eco53kI	GAGCTC	2 cut(s)	277, 487
Eco91I	GGTNACC	1 cut(s)	194
EcoICRI	GAGCTC	2 cut(s)	277, 487
EcoO65I	GGTNACC	1 cut(s)	194
EcoRII	CCWGG	1 cut(s)	615
EcoT14I	CCWWGG	1 cut(s)	256
EcoT38I	GRGCYC	2 cut(s)	279, 489
ErhI	CCWWGG	1 cut(s)	256
FaqI	GGGAC	1 cut(s)	632
FauNDI	CATATG	1 cut(s)	465
Fnu4HI	GCNGC	1 cut(s)	458
FokI	GGATG	1 cut(s)	152
FriOI	GRGCYC	2 cut(s)	279, 489
Fsp4HI	GCNGC	1 cut(s)	458
FspBI	CTAG	3 cut(s)	93, 257, 416
GluI	GCNGC	1 cut(s)	458
GsuI	CTGGAG	3 cut(s)	293, 595, 609
HaeIII	GGCC	3 cut(s)	157, 425, 460
HapII	CCGG	2 cut(s)	191, 512
HincII	GTYRAC	1 cut(s)	231
HindII	GTYRAC	1 cut(s)	231
HinfI	GANTC	3 cut(s)	114, 261, 331
HpaII	CCGG	2 cut(s)	191, 512
HphI	GGTGA	5 cut(s)	70, 340, 490, 525, 555
Hpy166II	GTNNAC	1 cut(s)	231
Hpy188I	TCNGA	3 cut(s)	70, 397, 431
Hpy188III	TCNNGA	7 cut(s)	120, 206, 217, 272, 323, 361, 416
Hpy8I	GTNNAC	1 cut(s)	231
HpyCH4III	ACNGT	2 cut(s)	554, 646
HpyCH4IV	ACGT	1 cut(s)	603
HpyCH4V	TGCA	3 cut(s)	128, 136, 146
HpyF10VI	GCNNNNNNNGC	3 cut(s)	344, 454, 466
HpyF3I	CTNAG	1 cut(s)	428
HpySE526I	ACGT	1 cut(s)	603
KroI	GCCGGC	1 cut(s)	511
KroNI	GCCGGC	1 cut(s)	513
Kzo9I	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
LmnI	GCTCC	3 cut(s)	274, 282, 484
LweI	GCATC	1 cut(s)	133
MaeI	CTAG	3 cut(s)	93, 257, 416
MaeII	ACGT	1 cut(s)	603
MaeIII	GTNAC	3 cut(s)	3, 194, 554
MalI	GATC	6 cut(s)	97, 152, 204, 365, 598, 630
MboI	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
MfeI	CAATTG	1 cut(s)	174
MflI	RGATCY	1 cut(s)	95
MhlI	GDGCHC	2 cut(s)	279, 489
MluCI	AATT	2 cut(s)	174, 297
MlyI	GAGTC	1 cut(s)	108
MroNI	GCCGGC	1 cut(s)	511
MroXI	GAANNNNTTC	3 cut(s)	75, 382, 411
MslI	CAYNNNNRTG	1 cut(s)	397
MspI	CCGG	2 cut(s)	191, 512
MspR9I	CCNGG	2 cut(s)	192, 617
MunI	CAATTG	1 cut(s)	174
Mva1269I	GAATGC	1 cut(s)	404
MvaI	CCWGG	1 cut(s)	617
MvnI	CGCG	1 cut(s)	121
MwoI	GCNNNNNNNGC	3 cut(s)	344, 454, 466
NaeI	GCCGGC	1 cut(s)	513
NciI	CCSGG	1 cut(s)	192
NdeI	CATATG	1 cut(s)	465
NdeII	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
NgoMIV	GCCGGC	1 cut(s)	511
NlaIV	GGNNCC	1 cut(s)	385
NmuCI	GTSAC	2 cut(s)	3, 554
NruI	TCGCGA	1 cut(s)	121
PctI	GAATGC	1 cut(s)	404
PdiI	GCCGGC	1 cut(s)	513
PdmI	GAANNNNTTC	3 cut(s)	75, 382, 411
PfeI	GAWTC	2 cut(s)	261, 331
PkrI	GCNGC	1 cut(s)	459
PleI	GAGTC	1 cut(s)	108
PpsI	GAGTC	1 cut(s)	108
Ppu21I	YACGTR	1 cut(s)	604
Psp124BI	GAGCTC	2 cut(s)	279, 489
Psp6I	CCWGG	1 cut(s)	615
PspEI	GGTNACC	1 cut(s)	194
PspGI	CCWGG	1 cut(s)	615
PspN4I	GGNNCC	1 cut(s)	385
PspPI	GGNCC	2 cut(s)	155, 424
PstI	CTGCAG	1 cut(s)	138
PsuI	RGATCY	1 cut(s)	95
RruI	TCGCGA	1 cut(s)	121
RsaI	GTAC	2 cut(s)	551, 643
RsaNI	GTAC	2 cut(s)	550, 642
RseI	CAYNNNNRTG	1 cut(s)	397
SacI	GAGCTC	2 cut(s)	279, 489
SatI	GCNGC	1 cut(s)	458
Sau3AI	GATC	6 cut(s)	95, 150, 202, 363, 596, 628
Sau96I	GGNCC	2 cut(s)	155, 424
SchI	GAGTC	1 cut(s)	108
ScrFI	CCNGG	2 cut(s)	192, 617
SduI	GDGCHC	2 cut(s)	279, 489
SfaNI	GCATC	1 cut(s)	133
SfcI	CTRYAG	2 cut(s)	134, 250
SmiMI	CAYNNNNRTG	1 cut(s)	397
Sse9I	AATT	2 cut(s)	174, 297
SsiI	CCGC	1 cut(s)	457
SspMI	CTAG	3 cut(s)	93, 257, 416
SstI	GAGCTC	2 cut(s)	279, 489
StyD4I	CCNGG	2 cut(s)	190, 615
StyI	CCWWGG	1 cut(s)	256
TaaI	ACNGT	2 cut(s)	554, 646
TaiI	ACGT	1 cut(s)	606
TaqI	TCGA	4 cut(s)	322, 362, 489, 664
TaqII	GACCGA	1 cut(s)	609
TasI	AATT	2 cut(s)	174, 297
TauI	GCSGC	1 cut(s)	460
TfiI	GAWTC	2 cut(s)	261, 331
TscAI	CASTG	2 cut(s)	559, 643
TseFI	GTSAC	2 cut(s)	3, 554
Tsp45I	GTSAC	2 cut(s)	3, 554
TspDTI	ATGAA	2 cut(s)	74, 456
TspRI	CASTG	2 cut(s)	559, 643
XbaI	TCTAGA	1 cut(s)	415
XmaJI	CCTAGG	1 cut(s)	256
XmnI	GAANNNNTTC	3 cut(s)	75, 382, 411
XspI	CTAG	3 cut(s)	93, 257, 416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.