Rh3BG100600
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002297
Physical Location & Seq
Reverse (-)
8030082 .. 8036931
6850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG100600.1

Sequence Viewer

Length: 2565 bp
ATGTTGAGACTGATATTTTGGGGTTGCCTCATTGGTCTGGCTTGTTCTTGCTTCTTCCTAGTTGCGGTTGCACAAGTTACTAATCCTTCTGAAGTGGATGCATTGAGAGCAGTGAAGGATAGTCTGATTGATTCCGGGAAGCATTTGGGGAATTGGGACAAAGGGGATCCATGTAAAGCGAATTGGACCGGAATTTTGTGCTCCGGTGATGTTGGGACTGATGGATACTTGCATGTCCAGGAGCTGCAACTGCTAAGAAAGAATCTCTCAGGAACGTTAGCGCCTGAGCTTGGCAAACTATCTCAACTTCTGATATTAGATTTCATGTGGAATAACTTAAGTGGGACTATACCGAAGGAGATAGGAAACATTACGTCCTTGAAACTCTTGCTCTTGAATGGAAACAAACTATCGGGTTCCTTGCCTGATGAGCTCGGTAATCTTGAAAATCTACATAGACTACAAGTTGACCAGAACCATCTTTCAGGTTCAATACCAAAATCATTTGTTAACTTGCGCGGCTTAAGACACCTCCATATGAACAACAACTCCTTCAGTGGCCAAATTCCATCTGAGATTTCTGAAATACCTGCTCTTCTTCATGTGCTCTTGGATAATAATAAATTATCTGGATATCTTCCACAAGAATTCTCCAACATTCCAGGGTTGCGCATACTTCAACTCGATAACAACAACTTCATGGGAACTGAGATTCCAGCTACTTATGGAAACCTTTCCAGATTGGCAAAGTTAAGTCTCAGGAACTGCAGTTTGCAGGGAGCAATTCCTGATCTTAGCCGGATACCTAACCTCAGTTATTTAGATCTAAGCTGGAATCATCTTGCTGGACCAATACCATCACATGGACTTTCTGACAATATGACAACAATTGACCTGTCAGATAATCATCTTAACGGATCTATTCCTGAAGGTTTCTCTCATTTTCCTCATCTTCAGAAATTGTCGCTCGAAAATAATTGGTTGAACGGTTCTGTCCCTGCTATATGGAGGAACATATCTTTTAGTACAAGAGCTAGACTTTCATTTGATCTCCGGAACAATTCACTCTCTAACATATTGGGAGAGCTGAATCCTCCAGCAAATGTCACATTGAGGCTAGAAGGAAATCCTATTTGCAAGAATGCTAGCATAGAAAATATAAGCCCGTTCTGTCAATCTGAAGCGAGAGATGGGATCCCCGAAGATTCAATAAATTCAACTCAACAGATGACCTGCCTTATTCAAGCATGTCCAGTGGATGATTTCTATGAATATGTTCCATCTTCCCCTGTGCCATGCTTTTGTGCATCACCTATTATAGTTGAATATCGTCTGAAAAGCCCGAGCTTTTCTTACTTTCTTCCATATATACAGCAGTTTGAAGAGTATTTAACTAGTTCTCTAAAATTGGAGATTTACCAGTTATCAATTAGTTCCTTTGTCTGGGAAAAAGGGCCTCGTTTACGGATGCATTTGAAGTTCTTCCCTGTGTATGGTGCTTCACACTCGCATACATTAAATGCAAGTGAGGTTCTGCGAATTAGAGGCATCTTTACATCATGGGAATTTCCTCAGAATGATTTCTTTGGACCATATGAACTACTCGACTTCAAACTGCTGGGACCTTATTCAAATATGATTGTCAAGAGACAAAAGATGGGCATCAATAAGGGTGTATTAGCAGCTGTTATAATAGGGGCCATTGCTTCTGTTGTCAGTATATCTGCAACAGTCATGCTTCTGTTCACAAGATGCACCCGAAACCGACACACACCACCTTCAAGGAGACACTCATCGTCGAAGATTTCTATGAGAATTGAAGGTGTAAAGGCGTTCACTTTCAAAGAAATGACACTAGCCACCAGGAACTTTGATAGTGCAACTCAACTTGGACGAGGAGGCTATGGGAAAGTTTATAAAGGTATCTTATCTGATAACACAGCTGTAGCCATAAAACGTGCAGAAGAAGGATCCCTACAGGGTGAAAAAGAATTCTTGACCGAGATAGAATTGTTATCAAGGCTACACCATCGAAACTTAGTCTCTTTGGTAGGATACTGTGAAGAAGAAGGGGAGCAGATGTTGGTTTATGAGTTCATGCCTAATGGTACCTTACGTGACTGGCTTTCTGATAAAACCAAGGGAAGCCTGGACTTCACACCTGTCCAGAATGATGAAGGGACTGGCCCTGCATACGTATCTACAGTCGTGAGAGGAACACCGGTGAATATGGCTCATCAGTCAGGCTTGATTTTCTCCATTATAGATAGCAGAATGGGTTCGTATCCATCTGAATGTGTTCAGAGGTTTGTAGCTTTGGCCCTCAGATGTTGCCATGATAAACAAGACGACCGGCCATCAATGTTGGATGTGGTGAGGGAGCTCGAGAACATAATCAAACTTATGCCGGGAGCTGAAACAATGTTTTCACCATCTGGTTCCTCATACACTGAAAAATCATTAACTTCATCATCGACTTTTACTGGGGACCCATATATGTCCTCCTCCAACGTGGAAGGAAGTGATCTAACCAGTGGTGTAATCCCTACCATCTCGCCTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

854

Amino Acids

94.62

Weight (kDa)

6.26

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 4.1e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 93 - 194 5e-09 Leucine-rich repeat region
LRR_14 PF23598 169 - 352 1.9e-07 Leucine-rich repeat region
Pkinase PF00069 626 - 713 2e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 628 - 713 5.5e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1691, 1917
Acc16I TGCGCA 1 cut(s) 671
Acc36I ACCTGC 2 cut(s) 598, 1241
Acc65I GGTACC 1 cut(s) 2108
AccB1I GGYRCC 1 cut(s) 2108
AccB7I CCANNNNNTGG 1 cut(s) 863
AccII CGCG 1 cut(s) 519
AccIII TCCGGA 1 cut(s) 1053
AciI CCGC 2 cut(s) 65, 519
AclI AACGTT 1 cut(s) 275
AclWI GGATC 7 cut(s) 161, 174, 925, 1189, 1202, 1965, 1978
AcoI YGGCCR 2 cut(s) 559, 2354
AcsI RAATTY 6 cut(s) 192, 564, 647, 1213, 1565, 1991
AcuI CTGAAG 5 cut(s) 111, 538, 938, 948, 1200
AfaI GTAC 2 cut(s) 1027, 2110
AfiI CCNNNNNNNGG 5 cut(s) 64, 290, 863, 1443, 1493
AflII CTTAAG 2 cut(s) 337, 523
AgeI ACCGGT 1 cut(s) 2221
AhlI ACTAGT 1 cut(s) 1394
AjnI CCWGG 4 cut(s) 237, 661, 1862, 2148
AloI GAACNNNNNNTCC 4 cut(s) 533, 565, 697, 729
Alw21I GWGCWC 4 cut(s) 203, 435, 609, 2385
Alw26I GTCTC 4 cut(s) 761, 1642, 1780, 2047
AlwI GGATC 7 cut(s) 161, 174, 925, 1189, 1202, 1965, 1978
AlwNI CAGNNNCTG 2 cut(s) 244, 765
Ama87I CYCGRG 2 cut(s) 1342, 2384
Aor13HI TCCGGA 1 cut(s) 1053
AoxI GGCC 6 cut(s) 559, 1454, 1698, 2185, 2319, 2354
ApeKI GCWGC 2 cut(s) 244, 1682
ApoI RAATTY 6 cut(s) 192, 564, 647, 1213, 1565, 1991
ArsI GACNNNNNNTTYG 3 cut(s) 32, 178, 210
AsiGI ACCGGT 1 cut(s) 2221
Asp700I GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2278, 2298
Asp718I GGTACC 1 cut(s) 2108
AspLEI GCGC 3 cut(s) 283, 519, 672
AspS9I GGNCC 9 cut(s) 186, 848, 1454, 1589, 1622, 1698, 2186, 2320, 2488
AsuC2I CCSGG 2 cut(s) 136, 2409
AsuHPI GGTGA 6 cut(s) 218, 1302, 1994, 2236, 2386, 2421
AsuNHI GCTAGC 1 cut(s) 1145
AvaI CYCGRG 2 cut(s) 1342, 2384
AvaII GGWCC 5 cut(s) 186, 848, 1589, 1622, 2488
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 3 cut(s) 166, 1194, 1970
BanI GGYRCC 1 cut(s) 2108
BanII GRGCYC 2 cut(s) 435, 2385
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
Bbv12I GWGCWC 4 cut(s) 203, 435, 609, 2385
BbvI GCAGC 2 cut(s) 231, 1694
BciT130I CCWGG 4 cut(s) 239, 663, 1864, 2150
BciVI GTATCC 4 cut(s) 218, 795, 2048, 2295
BcnI CCSGG 2 cut(s) 136, 2409
BcoDI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BcuI ACTAGT 1 cut(s) 1394
BfaI CTAG 6 cut(s) 59, 1035, 1118, 1146, 1395, 1856
BfmI CTRYAG 4 cut(s) 766, 1944, 1976, 2202
BfoI RGCGCY 1 cut(s) 284
BfrI CTTAAG 2 cut(s) 337, 523
BfuAI ACCTGC 2 cut(s) 598, 1241
BfuI GTATCC 4 cut(s) 218, 795, 2048, 2295
BglII AGATCT 1 cut(s) 823
BisI GCNGC 3 cut(s) 245, 520, 1683
BlsI GCNGC 3 cut(s) 246, 521, 1684
Bme1390I CCNGG 6 cut(s) 136, 239, 663, 1864, 2150, 2409
Bme18I GGWCC 5 cut(s) 186, 848, 1589, 1622, 2488
BmeT110I CYCGRG 2 cut(s) 1342, 2384
BmgT120I GGNCC 9 cut(s) 186, 848, 1454, 1589, 1622, 1698, 2186, 2320, 2488
BmrFI CCNGG 6 cut(s) 136, 239, 663, 1864, 2150, 2409
BmrI ACTGGG 1 cut(s) 2493
BmsI GCATC 6 cut(s) 88, 1316, 1458, 1557, 1671, 1742
BmtI GCTAGC 1 cut(s) 1149
BmuI ACTGGG 1 cut(s) 2493
BpmI CTGGAG 1 cut(s) 1080
Bpu10I CCTNAGC 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 136, 2409
BsaAI YACGTR 2 cut(s) 2117, 2197
BsaBI GATNNNNATC 2 cut(s) 906, 1661
BsaJI CCNNGG 3 cut(s) 662, 2139, 2558
BsaWI WCCGGW 4 cut(s) 188, 203, 1053, 2221
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 2066, 2096
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 290, 863, 1443, 1493
Bse118I RCCGGY 2 cut(s) 2221, 2352
Bse1I ACTGG 6 cut(s) 1253, 1420, 2126, 2188, 2488, 2532
Bse3DI GCAATG 1 cut(s) 1701
Bse8I GATNNNNATC 2 cut(s) 906, 1661
BseAI TCCGGA 1 cut(s) 1053
BseBI CCWGG 4 cut(s) 239, 663, 1864, 2150
BseDI CCNNGG 3 cut(s) 662, 2139, 2558
BseGI GGATG 4 cut(s) 103, 1264, 1473, 2374
BseJI GATNNNNATC 2 cut(s) 906, 1661
BseLI CCNNNNNNNGG 5 cut(s) 64, 290, 863, 1443, 1493
BseMI GCAATG 1 cut(s) 1701
BseMII CTCAG 8 cut(s) 276, 282, 564, 699, 772, 826, 1586, 2338
BseNI ACTGG 6 cut(s) 1253, 1420, 2126, 2188, 2488, 2532
BseRI GAGGAG 2 cut(s) 1911, 2494
BseXI GCAGC 2 cut(s) 231, 1694
BseYI CCCAGC 1 cut(s) 1618
BsgI GTGCAG 1 cut(s) 1980
Bsh1236I CGCG 1 cut(s) 519
Bsh1285I CGRYCG 1 cut(s) 2353
BshFI GGCC 6 cut(s) 561, 1456, 1700, 2187, 2321, 2356
BshNI GGYRCC 1 cut(s) 2108
BshTI ACCGGT 1 cut(s) 2221
BsiEI CGRYCG 1 cut(s) 2353
BsiHKAI GWGCWC 4 cut(s) 203, 435, 609, 2385
BsiHKCI CYCGRG 2 cut(s) 1342, 2384
BsiSI CCGG 8 cut(s) 135, 189, 204, 799, 1054, 2222, 2353, 2408
BslFI GGGAC 7 cut(s) 170, 229, 358, 980, 1635, 2194, 2501
BslI CCNNNNNNNGG 5 cut(s) 64, 290, 863, 1443, 1493
BsmAI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BsmFI GGGAC 7 cut(s) 170, 229, 358, 980, 1635, 2194, 2501
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 6 cut(s) 561, 1456, 1700, 2187, 2321, 2356
BsoBI CYCGRG 2 cut(s) 1342, 2384
Bsp1286I GDGCHC 4 cut(s) 203, 435, 609, 2385
Bsp13I TCCGGA 1 cut(s) 1053
Bsp143I GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
BspACI CCGC 2 cut(s) 65, 519
BspANI GGCC 6 cut(s) 561, 1456, 1700, 2187, 2321, 2356
BspCNI CTCAG 8 cut(s) 277, 281, 565, 700, 771, 825, 1585, 2337
BspEI TCCGGA 1 cut(s) 1053
BspFNI CGCG 1 cut(s) 519
BspMAI CTGCAG 1 cut(s) 770
BspMI ACCTGC 2 cut(s) 598, 1241
BspOI GCTAGC 1 cut(s) 1149
BspPI GGATC 7 cut(s) 161, 174, 925, 1189, 1202, 1965, 1978
BspQI GCTCTTC 1 cut(s) 600
BspT107I GGYRCC 1 cut(s) 2108
BspTI CTTAAG 2 cut(s) 337, 523
BsrDI GCAATG 1 cut(s) 1701
BsrFI RCCGGY 2 cut(s) 2221, 2352
BsrI ACTGG 6 cut(s) 1253, 1420, 2126, 2188, 2488, 2532
BssAI RCCGGY 2 cut(s) 2221, 2352
BssECI CCNNGG 3 cut(s) 662, 2139, 2558
BssMI GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
BssT1I CCWWGG 1 cut(s) 2139
Bst2UI CCWGG 4 cut(s) 239, 663, 1864, 2150
Bst4CI ACNGT 4 cut(s) 989, 1732, 2060, 2206
Bst6I CTCTTC 2 cut(s) 600, 1377
BstAFI CTTAAG 2 cut(s) 337, 523
BstBAI YACGTR 2 cut(s) 2117, 2197
BstC8I GCNNGC 1 cut(s) 1147
BstF5I GGATG 4 cut(s) 103, 1264, 1473, 2374
BstFNI CGCG 1 cut(s) 519
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 3 cut(s) 283, 519, 672
BstKTI GATC 8 cut(s) 169, 793, 826, 920, 1051, 1197, 1973, 2527
BstMAI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BstMBI GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
BstMCI CGRYCG 1 cut(s) 2353
BstMWI GCNNNNNNNGC 3 cut(s) 107, 250, 430
BstNI CCWGG 4 cut(s) 239, 663, 1864, 2150
BstNSI RCATGY 2 cut(s) 236, 1251
BstSCI CCNGG 6 cut(s) 134, 237, 661, 1862, 2148, 2407
BstSFI CTRYAG 4 cut(s) 766, 1944, 1976, 2202
BstSNI TACGTA 1 cut(s) 2197
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 2 cut(s) 231, 1694
BstX2I RGATCY 5 cut(s) 166, 823, 917, 1194, 1970
BstYI RGATCY 5 cut(s) 166, 823, 917, 1194, 1970
BsuI GTATCC 4 cut(s) 218, 795, 2048, 2295
BsuRI GGCC 6 cut(s) 561, 1456, 1700, 2187, 2321, 2356
BtsCI GGATG 4 cut(s) 103, 1264, 1473, 2374
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 5 cut(s) 117, 562, 1260, 2448, 2539
BveI ACCTGC 2 cut(s) 598, 1241
Cac8I GCNNGC 1 cut(s) 1147
CaiI CAGNNNCTG 2 cut(s) 244, 765
CfoI GCGC 3 cut(s) 283, 519, 672
Cfr10I RCCGGY 2 cut(s) 2221, 2352
Cfr13I GGNCC 9 cut(s) 186, 848, 1454, 1589, 1622, 1698, 2186, 2320, 2488
Csp6I GTAC 2 cut(s) 1026, 2109
CspAI ACCGGT 1 cut(s) 2221
CviQI GTAC 2 cut(s) 1026, 2109
DpnI GATC 8 cut(s) 168, 792, 825, 919, 1050, 1196, 1972, 2526
DpnII GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
EaeI YGGCCR 2 cut(s) 559, 2354
Eam1104I CTCTTC 2 cut(s) 600, 1377
EarI CTCTTC 2 cut(s) 600, 1377
Ecl136II GAGCTC 2 cut(s) 433, 2383
Eco105I TACGTA 1 cut(s) 2197
Eco130I CCWWGG 1 cut(s) 2139
Eco24I GRGCYC 2 cut(s) 435, 2385
Eco32I GATATC 1 cut(s) 635
Eco47I GGWCC 5 cut(s) 186, 848, 1589, 1622, 2488
Eco53kI GAGCTC 2 cut(s) 433, 2383
Eco57I CTGAAG 5 cut(s) 111, 538, 938, 948, 1200
Eco88I CYCGRG 2 cut(s) 1342, 2384
EcoICRI GAGCTC 2 cut(s) 433, 2383
EcoO109I RGGNCCY 3 cut(s) 1454, 1622, 2488
EcoRI GAATTC 2 cut(s) 647, 1991
EcoRII CCWGG 4 cut(s) 237, 661, 1862, 2148
EcoRV GATATC 1 cut(s) 635
EcoT14I CCWWGG 1 cut(s) 2139
EcoT22I ATGCAT 2 cut(s) 103, 1473
EcoT38I GRGCYC 2 cut(s) 435, 2385
ErhI CCWWGG 1 cut(s) 2139
FaqI GGGAC 7 cut(s) 170, 229, 358, 980, 1635, 2194, 2501
FauNDI CATATG 2 cut(s) 537, 1594
Fnu4HI GCNGC 3 cut(s) 245, 520, 1683
FokI GGATG 4 cut(s) 110, 1271, 1480, 2381
FriOI GRGCYC 2 cut(s) 435, 2385
Fsp4HI GCNGC 3 cut(s) 245, 520, 1683
FspBI CTAG 6 cut(s) 59, 1035, 1118, 1146, 1395, 1856
FspI TGCGCA 1 cut(s) 671
GlaI GCGC 3 cut(s) 282, 518, 671
GluI GCNGC 3 cut(s) 245, 520, 1683
GsaI CCCAGC 1 cut(s) 1622
GsuI CTGGAG 1 cut(s) 1080
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 6 cut(s) 561, 1456, 1700, 2187, 2321, 2356
HapII CCGG 8 cut(s) 135, 189, 204, 799, 1054, 2222, 2353, 2408
HhaI GCGC 3 cut(s) 283, 519, 672
Hin6I GCGC 3 cut(s) 281, 517, 670
HinP1I GCGC 3 cut(s) 281, 517, 670
HincII GTYRAC 2 cut(s) 469, 511
HindII GTYRAC 2 cut(s) 469, 511
HinfI GANTC 6 cut(s) 131, 262, 712, 835, 1090, 1205
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 8 cut(s) 135, 189, 204, 799, 1054, 2222, 2353, 2408
HphI GGTGA 6 cut(s) 218, 1302, 1994, 2236, 2386, 2421
Hpy166II GTNNAC 5 cut(s) 469, 511, 1463, 1746, 1836
Hpy8I GTNNAC 5 cut(s) 469, 511, 1463, 1746, 1836
Hpy99I CGWCG 1 cut(s) 1801
HpyCH4III ACNGT 4 cut(s) 989, 1732, 2060, 2206
HpyCH4IV ACGT 6 cut(s) 275, 374, 1957, 2116, 2196, 2511
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 250, 430
HpySE526I ACGT 6 cut(s) 275, 374, 1957, 2116, 2196, 2511
HspAI GCGC 3 cut(s) 281, 517, 670
KflI GGGWCCC 1 cut(s) 2488
Kpn2I TCCGGA 1 cut(s) 1053
KpnI GGTACC 1 cut(s) 2112
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 6 cut(s) 206, 241, 779, 2074, 2380, 2411
Lsp1109I GCAGC 2 cut(s) 231, 1694
LweI GCATC 6 cut(s) 88, 1316, 1458, 1557, 1671, 1742
MaeI CTAG 6 cut(s) 59, 1035, 1118, 1146, 1395, 1856
MaeII ACGT 6 cut(s) 275, 374, 1957, 2116, 2196, 2511
MaeIII GTNAC 3 cut(s) 76, 1105, 2117
MalI GATC 8 cut(s) 168, 792, 825, 919, 1050, 1196, 1972, 2526
MboI GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 5 cut(s) 166, 823, 917, 1194, 1970
MhlI GDGCHC 4 cut(s) 203, 435, 609, 2385
MlsI TGGCCA 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 561
MmeI TCCRAC 3 cut(s) 678, 2346, 2532
Mox20I TGGCCA 1 cut(s) 561
Mph1103I ATGCAT 2 cut(s) 103, 1473
MroI TCCGGA 1 cut(s) 1053
MroXI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2278, 2298
MscI TGGCCA 1 cut(s) 561
MseI TTAA 8 cut(s) 338, 510, 524, 752, 912, 1391, 1517, 2462
MslI CAYNNNNRTG 1 cut(s) 2293
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 2 cut(s) 1685, 1943
MspCI CTTAAG 2 cut(s) 337, 523
MspI CCGG 8 cut(s) 135, 189, 204, 799, 1054, 2222, 2353, 2408
MspR9I CCNGG 6 cut(s) 136, 239, 663, 1864, 2150, 2409
MunI CAATTG 1 cut(s) 888
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 4 cut(s) 239, 663, 1864, 2150
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 3 cut(s) 107, 250, 430
NciI CCSGG 2 cut(s) 136, 2409
NdeI CATATG 2 cut(s) 537, 1594
NdeII GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
NheI GCTAGC 1 cut(s) 1145
NmuCI GTSAC 2 cut(s) 1105, 2117
NsbI TGCGCA 1 cut(s) 671
NsiI ATGCAT 2 cut(s) 103, 1473
NspI RCATGY 2 cut(s) 236, 1251
PaeR7I CTCGAG 1 cut(s) 2384
PciSI GCTCTTC 1 cut(s) 600
PctI GAATGC 1 cut(s) 1147
PdmI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2278, 2298
PfeI GAWTC 6 cut(s) 131, 262, 712, 835, 1090, 1205
PflMI CCANNNNNTGG 1 cut(s) 863
PfoI TCCNGGA 2 cut(s) 134, 237
PinAI ACCGGT 1 cut(s) 2221
PkrI GCNGC 3 cut(s) 246, 521, 1684
Ppu21I YACGTR 2 cut(s) 2117, 2197
PpuMI RGGWCCY 2 cut(s) 1622, 2488
PsiI TTATAA 2 cut(s) 1691, 1917
Psp124BI GAGCTC 2 cut(s) 435, 2385
Psp1406I AACGTT 1 cut(s) 275
Psp5II RGGWCCY 2 cut(s) 1622, 2488
Psp6I CCWGG 4 cut(s) 237, 661, 1862, 2148
PspFI CCCAGC 1 cut(s) 1618
PspGI CCWGG 4 cut(s) 237, 661, 1862, 2148
PspPI GGNCC 9 cut(s) 186, 848, 1454, 1589, 1622, 1698, 2186, 2320, 2488
PspPPI RGGWCCY 2 cut(s) 1622, 2488
PstI CTGCAG 1 cut(s) 770
PstNI CAGNNNCTG 2 cut(s) 244, 765
PsuI RGATCY 5 cut(s) 166, 823, 917, 1194, 1970
PvuII CAGCTG 2 cut(s) 1685, 1943
RsaI GTAC 2 cut(s) 1027, 2110
RsaNI GTAC 2 cut(s) 1026, 2109
RseI CAYNNNNRTG 1 cut(s) 2293
SacI GAGCTC 2 cut(s) 435, 2385
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 8 cut(s) 338, 510, 524, 752, 912, 1391, 1517, 2462
SatI GCNGC 3 cut(s) 245, 520, 1683
Sau3AI GATC 8 cut(s) 166, 790, 823, 917, 1048, 1194, 1970, 2524
Sau96I GGNCC 9 cut(s) 186, 848, 1454, 1589, 1622, 1698, 2186, 2320, 2488
ScrFI CCNGG 6 cut(s) 136, 239, 663, 1864, 2150, 2409
SduI GDGCHC 4 cut(s) 203, 435, 609, 2385
SfaNI GCATC 6 cut(s) 88, 1316, 1458, 1557, 1671, 1742
SfcI CTRYAG 4 cut(s) 766, 1944, 1976, 2202
Sfr274I CTCGAG 1 cut(s) 2384
SgrAI CRCCGGYG 1 cut(s) 2221
SinI GGWCC 5 cut(s) 186, 848, 1589, 1622, 2488
SlaI CTCGAG 1 cut(s) 2384
SmiMI CAYNNNNRTG 1 cut(s) 2293
SmlI CTYRAG 3 cut(s) 337, 523, 2384
SmoI CTYRAG 3 cut(s) 337, 523, 2384
SnaBI TACGTA 1 cut(s) 2197
SpeI ACTAGT 1 cut(s) 1394
SsiI CCGC 2 cut(s) 65, 519
SspMI CTAG 6 cut(s) 59, 1035, 1118, 1146, 1395, 1856
SstI GAGCTC 2 cut(s) 435, 2385
StyD4I CCNGG 6 cut(s) 134, 237, 661, 1862, 2148, 2407
StyI CCWWGG 1 cut(s) 2139
TaaI ACNGT 4 cut(s) 989, 1732, 2060, 2206
TaiI ACGT 6 cut(s) 278, 377, 1960, 2119, 2199, 2514
TaqI TCGA 7 cut(s) 684, 969, 1605, 1799, 2032, 2385, 2474
TaqII GACCGA 1 cut(s) 2015
TatI WGTACW 1 cut(s) 1025
TauI GCSGC 1 cut(s) 522
TfiI GAWTC 6 cut(s) 131, 262, 712, 835, 1090, 1205
Tru1I TTAA 8 cut(s) 338, 510, 524, 752, 912, 1391, 1517, 2462
Tru9I TTAA 8 cut(s) 338, 510, 524, 752, 912, 1391, 1517, 2462
TscAI CASTG 5 cut(s) 117, 562, 1260, 2455, 2539
TseFI GTSAC 2 cut(s) 1105, 2117
TseI GCWGC 2 cut(s) 244, 1682
Tsp45I GTSAC 2 cut(s) 1105, 2117
TspGWI ACGGA 2 cut(s) 930, 1480
TspRI CASTG 5 cut(s) 117, 562, 1260, 2455, 2539
Van91I CCANNNNNTGG 1 cut(s) 863
Vha464I CTTAAG 2 cut(s) 337, 523
VpaK11BI GGWCC 5 cut(s) 186, 848, 1589, 1622, 2488
XapI RAATTY 6 cut(s) 192, 564, 647, 1213, 1565, 1991
XceI RCATGY 2 cut(s) 236, 1251
XcmI CCANNNNNNNNNTGG 1 cut(s) 2146
XhoI CTCGAG 1 cut(s) 2384
XmnI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2278, 2298
XspI CTAG 6 cut(s) 59, 1035, 1118, 1146, 1395, 1856
Zsp2I ATGCAT 2 cut(s) 103, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.