Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001873

Physical Location & Seq

Genomic Coordinates Forward (+)

9114431 .. 9120484

Gene Structure 6054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g14760.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3195 bp

ATGGGGAAAGGAGGTGAGGATTTCGGTAAGCGCGAGAAGACGGCGGCCGGTCCGGCGACGACGTCCGAGAGCGATGTGTTTCCGGCGTGGAGCAAGGAGATTCACGAGTGCGAGAAGCACTTCGGTGTGAACAGGAAGGTAGGGTTGACGTCGGATGAGGTGGCGAAGCGGCGGGAGGAGTATGGATTGAACGAGCTGGAGAAGCACGAGGGGCAGTCGATTTGGAGCCTGGTGCTGGAGCAGTTCAATGATACTCTGGTGAGGATCCTCCTCGCCGCGGCGGTGATCTCGTTTGTGCTGGCGTGGTTGGACGGCGATGAAGGCGGCGAGAAGGAGATCACGGCGTTTGTGGAGCCGTTGGTGATTTTTTTGATCTTGATTGTGAACGCGATTGTGGGAGTTTGGCAGGAGAGTAATGCCGAAAAGGCGCTGGAGGCGCTTAAGGAGATTCAGTCGGAGCAGGCCACTGTGATTCGGAATGGGAGTAAGATTCGGAATTTGCCTGCGAAGGAGCTTGTTCCTGGAGATATTGTGGAGCTTAAGGTTGGGGATAAGGTTCCTGCCGATATGCGTGTAGTGGAGTTGATTAGTTCGACTTTGAGGGTTGAGCAGGGCTCATTGACTGGGGAGAGTGAGGCTGTGAATAAGACTAACAAGCCGGTTTCGGAGGATGCTGATATTCAGGGGAAGTGGTCTATGGTGTTTGCGGGGACGACTATTGTGAATGGGAATTGCATTTGCTTGGTTGCGCAGACGGGGATGGCCACCGAGATTGGGAAGGTGCATATGCAAATTCATGTGGCGTCGCAGAGTGAGGAAGACACTCCGTTGAAGAAGAAGCTTAATGAGTTTGGGGAGATGCTCACTATGATAATTGGAGTGATTTGTGCATTGGTGTGGCTCATCAATGTCAAGTACTTCCTCACTTGGGATTATGTGGATGGGATGCCTAGGAACTTCAAGTTTTCATTTGAGAAGTGCACTTATTACTTCGAGATTGCAGTGGCATTGGCTGTGGCTGCTATCCCTGAAGGTCTGCCTGCAGTCATCACTACCTGCTTGGCTCTTGGTACCCGCAAAATGGCCCAGAAGAATGCCTTGGTTAGAAAGCTTCCCAGTGTTGAAACTCTGGGTTGTACCACAGTTATTTGTTCTGACAAAACAGGGACCTTGACTACGAACCAAATGGCAGTTTCCAGGCTTGTAGCTTTGGGTCCTAGACCTACTATACTACGGAAGTTTAAGGTGGACGGGACTACATACAATCCAGCAGATGGCAAAATTCATGACTGGCCAACTGGACGGATGGATACTAATCTTCAGACCATCGCAAAGGTAGCTGCTGTCTGTAATGATGCTGGTATTACCCAATCAGAACAGAAGTATGTTTCCCATGGAATGCCTACCGAGGCAGCTCTTAAGGTTTTAGTTGAGAAGATGGGGCTTCCTGAAGCATCTCGAGGTGTGGGATCAAATAAAACCGACTTATTAGGTTGTTGCCAGCAGTGGAATGAATCCGAGCGCCGAGTTGCAACACTTGAGTTTGATCGTGATAGGAAATCTATGGGTGTCATTGCCACCTCCCGTTCAGGGAAAAATTCTTTGCTGGTGAAGGGTGCTGTAGAAAATGTGTTGGAGAGAAGTACTCAGGTTCAGTTGCTTGATGGAACTGTTGTACCACTAGATAATAACTCAAGGAACTACATCTTACAAGCTCTTAATGAGATGTCATCTGAGGCACTACGTTGTCTGGGCTTTGCATACAAAGATGATTTGGGCGACTTTGAATCCTATGATGGTGATGAACATCCAGCTCACAAGCAATTACTTGATCCATCCAATTATTCATCGATAGAGAGCGAACTTGTTTTTGTGGGATTGGTTGGACTGAGGGATCCGCCAAGAGAGGAGGTTTTTGATGCAATCGAAGATTGCCGTGCAGCTGGAATTCGTGTTATGGTAATTACTGGAGATAACAAGAACACCGCTGAAGCAATATGTCGTGAAATAGGTGTTTTTGGTACTCATGAAGAGATCAAGTCAAGAAGCATAACAGGAAGAGAATTTATGAATCATGCTGATCAGAAAGGCTTTCTGAGACAAGGTGGTGGGCTACTTTTTTCACGGGCTGAGCCAAGACACAAACAAGAGATTGTGAGGTTGCTGAAAGAGGACGGTGAGGTGGTTGCCATGACTGGTGATGGAGTGAATGATGCTCCTGCTTTGAAACTTGCTGATATTGGAATTGCAATGGGCATTGCTGGAACCGAGGTTGCGAAGGAAGCTTCCGACATGGTTTTGGCTGATGATAATTTCAGCACAATTGTTGCTGCGGTTGGTGAGGGAAGATCAATCTACAACAACATGAAGGCCTTTATCAGGTATATGATATCTTCCAATATTGGTGAGGTTGCATCTATATTCCTAACAGCAGCATTGGGCATTCCTGAAGGGCTCATCCCTGTCCAGCTTTTGTGGGTCAATCTTGTCACTGATGGGCCACCTGCAACAGCATTAGGATTTAATCCTCCGGACAAGGACATAATGAAGAAACCCCCACGCAGAAGTGATGATTCACTTATCAGTGCTTGGATATTGTTCCGATATTTGGTAATTGGGTTGTATGTTGGAGTAGCAACAGTTGGTGTGTTCATCATTTGGTATACTCATGGTTCCTTCTTGGGTATTGACCTCAGTGGGGATGGCCACTCCCTTGTTACTTACTCCCAACTTTCCAACTGGGGACAGTGCTCAACATGGCAGAACTTTACTGCTTCACCCTTTACAGCAGGAAGCCAGGTTATCTCCTTTGACAACAACCCATGTGATTATTTCCATGGCGGCAAAGTTAAGGCTATGACCCTCTCCCTCTCTGTGTTGGTTGCAATCGAAATGTTCAACTCGCTTAATGCCCTTTCTGAGGATGGAAGCTTATTGTCAATGCCCCCGTGGGTCAACCCGTGGCTCCTTGTGGCTATGTCTGTTTCATTTGGCTCTCATTTCTTGATCTTGTATGTGCCTTTCCTGGCTCAAATATTCGGCATTGTGCCCCTGAGCCTAAACGAATGGCTGTTGGTTTTGGCTGTTTCATTGCCGGTGATACTGATCGATGAGGTCCTTAAGTTAGTTGGCAGATGGATACACAAGTCCGAAACGACCAGAAGGAGAAAACCATCAAAGCCCAAGGCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010042 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1065

Amino Acids

116.36

Weight (kDa)

5.35

Isoelectric Point (pI)

38.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	30 - 97	1.2e-20	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	150 - 262	1.1e-23	P-type ATPase actuator domain
Cation_ATPase	PF13246	440 - 637	1.1e-42	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	625 - 746	3.5e-15	haloacid dehalogenase-like hydrolase
HAD	PF12710	631 - 743	2.5e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	818 - 1043	5.8e-49	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07670 AT1G07670 AT1G07810
fragaria_vesca	FvH4_3g34570 FvH4_3g34570 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760
malus_domestica	MD04G1038900.v1.1 MD04G1116400.v1.1 MD11G1117700.v1.1 MD12G1132100.v1.1 MD13G1142700.v1.1
prunus_persica	Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1
pyrus_communis	pycom03g12390 pycom03g12400 pycom04g10640 pycom07g10780 pycom11g09870 pycom12g12810 pycom16g18850
rosa_chinensis	RchiOBHm_Chr3g0467981 RchiOBHm_Chr5g0062231
rosa_laevigata	RLG00000024462 RLG00000035545
rosa_multiflora	Rmu_co8021382.1_g000001 Rmu_sc0001793.1_g000060 Rmu_ssc0000409.1_g000035
rosa_roxburghii	Rroxscaffold_1G00018460 Rroxscaffold_6G00413240
rosa_rugosa	Rorug03G0090700 Rorug03G0090800 Rorug05G0347300
rosa_samantha	Rh3AG139600 Rh3BG161100 Rh3CG161000 Rh3DG161500 Rh5AG407900 Rh5BG421600 Rh5CG445600 Rh5DG436600
rosa_wichuraiana	Rw3G013200 Rw5G038440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2512
AatII	GACGTC	2 cut(s)	65, 152
Acc16I	TGCGCA	1 cut(s)	750
Acc36I	ACCTGC	2 cut(s)	1064, 2512
Acc65I	GGTACC	1 cut(s)	1070
AccB1I	GGYRCC	1 cut(s)	1070
AccB7I	CCANNNNNTGG	1 cut(s)	1274
AccI	GTMKAC	1 cut(s)	2663
AccII	CGCG	3 cut(s)	33, 278, 389
AccIII	TCCGGA	1 cut(s)	2530
AclWI	GGATC	6 cut(s)	259, 272, 1477, 1826, 1889, 1902
AcoI	YGGCCR	4 cut(s)	45, 762, 1292, 2704
AcsI	RAATTY	6 cut(s)	496, 792, 1281, 1597, 1947, 2063
AcuI	CTGAAG	5 cut(s)	1050, 1304, 1470, 2010, 2469
AcyI	GRCGYC	3 cut(s)	62, 149, 803
AfaI	GTAC	6 cut(s)	917, 1072, 1138, 1645, 1677, 2023
AflII	CTTAAG	4 cut(s)	440, 539, 1418, 3119
AgsI	TTSAA	8 cut(s)	190, 247, 832, 961, 1124, 1787, 2227, 2899
AjnI	CCWGG	5 cut(s)	228, 520, 1196, 2796, 3024
AjuI	GAANNNNNNNTTGG	2 cut(s)	1082, 1114
AleI	CACNNNNGTG	1 cut(s)	123
Alw21I	GWGCWC	2 cut(s)	983, 2753
Alw26I	GTCTC	1 cut(s)	2092
Alw44I	GTGCAC	1 cut(s)	979
AlwI	GGATC	6 cut(s)	259, 272, 1477, 1826, 1889, 1902
Ama87I	CYCGRG	1 cut(s)	1458
Aor13HI	TCCGGA	1 cut(s)	2530
AoxI	GGCC	8 cut(s)	45, 462, 762, 1083, 1292, 2370, 2498, 2704
ApaLI	GTGCAC	1 cut(s)	979
ApeKI	GCWGC	6 cut(s)	1019, 1340, 1412, 1940, 2330, 2432
ApoI	RAATTY	6 cut(s)	496, 792, 1281, 1597, 1947, 2063
Asp700I	GAANNNNTTC	1 cut(s)	119
Asp718I	GGTACC	1 cut(s)	1070
AspA2I	CCTAGG	1 cut(s)	950
AspLEI	GCGC	5 cut(s)	33, 430, 439, 751, 1524
AspS9I	GGNCC	6 cut(s)	50, 1084, 1167, 1214, 2498, 3115
AvaI	CYCGRG	1 cut(s)	1458
AvaII	GGWCC	4 cut(s)	50, 1167, 1214, 3115
AvrII	CCTAGG	1 cut(s)	950
BaeGI	GKGCMC	2 cut(s)	983, 3051
BaeI	ACNNNNGTAYC	6 cut(s)	243, 276, 1659, 1692, 3131, 3164
BalI	TGGCCA	3 cut(s)	764, 1294, 2706
BamHI	GGATCC	2 cut(s)	264, 1894
BanI	GGYRCC	1 cut(s)	1070
BanII	GRGCYC	2 cut(s)	617, 2457
BauI	CACGAG	2 cut(s)	104, 206
BbsI	GAAGAC	2 cut(s)	44, 825
Bbv12I	GWGCWC	2 cut(s)	983, 2753
BbvI	GCAGC	6 cut(s)	1006, 1327, 1424, 1952, 2317, 2444
BceAI	ACGGC	5 cut(s)	57, 328, 340, 357, 1920
BciT130I	CCWGG	5 cut(s)	230, 522, 1198, 2798, 3026
BciVI	GTATCC	2 cut(s)	1303, 3132
BclI	TGATCA	1 cut(s)	2080
BcoDI	GTCTC	1 cut(s)	2092
BfaI	CTAG	3 cut(s)	951, 1218, 1682
BfmI	CTRYAG	2 cut(s)	1041, 1620
BfoI	RGCGCY	3 cut(s)	431, 440, 1525
BfrI	CTTAAG	4 cut(s)	440, 539, 1418, 3119
BfuAI	ACCTGC	2 cut(s)	1064, 2512
BfuI	GTATCC	2 cut(s)	1303, 3132
BglI	GCCNNNNNGGC	2 cut(s)	53, 425
BlnI	CCTAGG	1 cut(s)	950
BlpI	GCTNAGC	1 cut(s)	2130
BmcAI	AGTACT	2 cut(s)	917, 1645
Bme1390I	CCNGG	5 cut(s)	230, 522, 1198, 2798, 3026
Bme18I	GGWCC	4 cut(s)	50, 1167, 1214, 3115
BmeT110I	CYCGRG	1 cut(s)	1458
BmgT120I	GGNCC	6 cut(s)	50, 1084, 1167, 1214, 2498, 3115
BmrFI	CCNGG	5 cut(s)	230, 522, 1198, 2798, 3026
BmrI	ACTGGG	3 cut(s)	633, 1110, 2749
BmsI	GCATC	8 cut(s)	661, 849, 936, 1345, 1463, 1909, 2203, 2423
BmuI	ACTGGG	3 cut(s)	633, 1110, 2749
BpiI	GAAGAC	2 cut(s)	44, 825
BplI	GAGNNNNNCTC	4 cut(s)	599, 631, 1630, 1662
BpmI	CTGGAG	5 cut(s)	218, 257, 452, 543, 1989
Bpu10I	CCTNAGC	1 cut(s)	3053
Bpu1102I	GCTNAGC	1 cut(s)	2130
BpuEI	CTTGAG	2 cut(s)	1559, 1678
Bsa29I	ATCGAT	2 cut(s)	1850, 3108
BsaBI	GATNNNNATC	3 cut(s)	1314, 1806, 3104
BsaHI	GRCGYC	3 cut(s)	62, 149, 803
BsaWI	WCCGGW	1 cut(s)	2530
BsaXI	ACNNNNNCTCC	4 cut(s)	437, 467, 516, 546
Bse118I	RCCGGY	3 cut(s)	47, 658, 3094
Bse1I	ACTGG	7 cut(s)	628, 1116, 1295, 1303, 1972, 2200, 2744
Bse3DI	GCAATG	4 cut(s)	1572, 2256, 2256, 3089
Bse8I	GATNNNNATC	3 cut(s)	1314, 1806, 3104
BseAI	TCCGGA	1 cut(s)	2530
BseBI	CCWGG	5 cut(s)	230, 522, 1198, 2798, 3026
BseCI	ATCGAT	2 cut(s)	1850, 3108
BseJI	GATNNNNATC	3 cut(s)	1314, 1806, 3104
BseMI	GCAATG	4 cut(s)	1572, 2256, 2256, 3089
BseMII	CTCAG	8 cut(s)	1661, 1725, 1880, 2087, 2121, 2707, 2910, 3044
BseNI	ACTGG	7 cut(s)	628, 1116, 1295, 1303, 1972, 2200, 2744
BseRI	GAGGAG	3 cut(s)	191, 260, 1922
BseSI	GKGCMC	2 cut(s)	983, 3051
BseX3I	CGGCCG	1 cut(s)	45
BseXI	GCAGC	6 cut(s)	1006, 1327, 1424, 1952, 2317, 2444
BsgI	GTGCAG	1 cut(s)	1959
Bsh1236I	CGCG	3 cut(s)	33, 278, 389
Bsh1285I	CGRYCG	1 cut(s)	48
BshFI	GGCC	8 cut(s)	47, 464, 764, 1085, 1294, 2372, 2500, 2706
BshNI	GGYRCC	1 cut(s)	1070
BshVI	ATCGAT	2 cut(s)	1850, 3108
BsiEI	CGRYCG	1 cut(s)	48
BsiHKAI	GWGCWC	2 cut(s)	983, 2753
BsiHKCI	CYCGRG	1 cut(s)	1458
BsiSI	CCGG	6 cut(s)	48, 53, 83, 659, 2531, 3095
BslFI	GGGAC	4 cut(s)	724, 1180, 1267, 2757
BsmAI	GTCTC	1 cut(s)	2092
BsmFI	GGGAC	4 cut(s)	724, 1180, 1267, 2757
BsmI	GAATGC	3 cut(s)	1099, 1404, 2442
BsnI	GGCC	8 cut(s)	47, 464, 764, 1085, 1294, 2372, 2500, 2706
BsoBI	CYCGRG	1 cut(s)	1458
Bsp1286I	GDGCHC	5 cut(s)	617, 983, 2457, 2753, 3051
Bsp13I	TCCGGA	1 cut(s)	2530
Bsp1720I	GCTNAGC	1 cut(s)	2130
Bsp19I	CCATGG	2 cut(s)	1393, 2836
BspANI	GGCC	8 cut(s)	47, 464, 764, 1085, 1294, 2372, 2500, 2706
BspCNI	CTCAG	8 cut(s)	1660, 1726, 1881, 2088, 2122, 2706, 2911, 3045
BspDI	ATCGAT	2 cut(s)	1850, 3108
BspEI	TCCGGA	1 cut(s)	2530
BspFNI	CGCG	3 cut(s)	33, 278, 389
BspHI	TCATGA	2 cut(s)	1285, 2026
BspMAI	CTGCAG	1 cut(s)	1045
BspMI	ACCTGC	2 cut(s)	1064, 2512
BspPI	GGATC	6 cut(s)	259, 272, 1477, 1826, 1889, 1902
BspT107I	GGYRCC	1 cut(s)	1070
BspTI	CTTAAG	4 cut(s)	440, 539, 1418, 3119
BsrDI	GCAATG	4 cut(s)	1572, 2256, 2256, 3089
BsrFI	RCCGGY	3 cut(s)	47, 658, 3094
BsrI	ACTGG	7 cut(s)	628, 1116, 1295, 1303, 1972, 2200, 2744
BssAI	RCCGGY	3 cut(s)	47, 658, 3094
BssNAI	GTATAC	1 cut(s)	2664
BssNI	GRCGYC	3 cut(s)	62, 149, 803
BssSI	CACGAG	2 cut(s)	104, 206
BssT1I	CCWWGG	5 cut(s)	950, 1098, 1393, 2836, 3183
Bst1107I	GTATAC	1 cut(s)	2664
Bst2BI	CACGAG	2 cut(s)	104, 206
Bst2UI	CCWGG	5 cut(s)	230, 522, 1198, 2798, 3026
Bst4CI	ACNGT	6 cut(s)	469, 1144, 1672, 2177, 2641, 2748
Bst6I	CTCTTC	2 cut(s)	2025, 2053
BstACI	GRCGYC	3 cut(s)	62, 149, 803
BstAFI	CTTAAG	4 cut(s)	440, 539, 1418, 3119
BstC8I	GCNNGC	5 cut(s)	300, 462, 504, 1041, 1502
BstDEI	CTNAG	8 cut(s)	1647, 1734, 1889, 2096, 2130, 2693, 2919, 3053
BstDSI	CCRYGG	5 cut(s)	276, 1393, 2836, 2948, 2960
BstENI	CCTNNNNNAGG	2 cut(s)	2377, 2918
BstFNI	CGCG	3 cut(s)	33, 278, 389
BstH2I	RGCGCY	3 cut(s)	431, 440, 1525
BstHHI	GCGC	5 cut(s)	33, 430, 439, 751, 1524
BstMAI	GTCTC	1 cut(s)	2092
BstMCI	CGRYCG	1 cut(s)	48
BstNI	CCWGG	5 cut(s)	230, 522, 1198, 2798, 3026
BstSCI	CCNGG	5 cut(s)	228, 520, 1196, 2796, 3024
BstSFI	CTRYAG	2 cut(s)	1041, 1620
BstSLI	GKGCMC	2 cut(s)	983, 3051
BstUI	CGCG	3 cut(s)	33, 278, 389
BstV1I	GCAGC	6 cut(s)	1006, 1327, 1424, 1952, 2317, 2444
BstV2I	GAAGAC	2 cut(s)	44, 825
BstX2I	RGATCY	2 cut(s)	264, 1894
BstYI	RGATCY	2 cut(s)	264, 1894
BstZ17I	GTATAC	1 cut(s)	2664
BstZI	CGGCCG	1 cut(s)	45
Bsu15I	ATCGAT	2 cut(s)	1850, 3108
BsuI	GTATCC	2 cut(s)	1303, 3132
BsuRI	GGCC	8 cut(s)	47, 464, 764, 1085, 1294, 2372, 2500, 2706
BsuTUI	ATCGAT	2 cut(s)	1850, 3108
BtgI	CCRYGG	5 cut(s)	276, 1393, 2836, 2948, 2960
BtgZI	GCGATG	3 cut(s)	87, 330, 1312
BtsI	GCAGTG	2 cut(s)	1008, 1511
BtsIMutI	CAGTG	8 cut(s)	465, 1008, 1123, 1511, 2490, 2590, 2701, 2753
BveI	ACCTGC	2 cut(s)	1064, 2512
Cac8I	GCNNGC	5 cut(s)	300, 462, 504, 1041, 1502
CciI	TCATGA	2 cut(s)	1285, 2026
CfoI	GCGC	5 cut(s)	33, 430, 439, 751, 1524
Cfr10I	RCCGGY	3 cut(s)	47, 658, 3094
Cfr13I	GGNCC	6 cut(s)	50, 1084, 1167, 1214, 2498, 3115
Cfr42I	CCGCGG	1 cut(s)	279
ClaI	ATCGAT	2 cut(s)	1850, 3108
CpoI	CGGWCCG	1 cut(s)	50
CseI	GACGC	1 cut(s)	792
Csp6I	GTAC	6 cut(s)	916, 1071, 1137, 1644, 1676, 2022
CspCI	CAANNNNNGTGG	2 cut(s)	754, 789
CspI	CGGWCCG	1 cut(s)	50
CviQI	GTAC	6 cut(s)	916, 1071, 1137, 1644, 1676, 2022
DdeI	CTNAG	8 cut(s)	1647, 1734, 1889, 2096, 2130, 2693, 2919, 3053
EaeI	YGGCCR	4 cut(s)	45, 762, 1292, 2704
EagI	CGGCCG	1 cut(s)	45
Eam1104I	CTCTTC	2 cut(s)	2025, 2053
EarI	CTCTTC	2 cut(s)	2025, 2053
EciI	GGCGGA	1 cut(s)	1887
EclXI	CGGCCG	1 cut(s)	45
Eco130I	CCWWGG	5 cut(s)	950, 1098, 1393, 2836, 3183
Eco147I	AGGCCT	1 cut(s)	2372
Eco24I	GRGCYC	2 cut(s)	617, 2457
Eco32I	GATATC	1 cut(s)	2391
Eco47I	GGWCC	4 cut(s)	50, 1167, 1214, 3115
Eco52I	CGGCCG	1 cut(s)	45
Eco57I	CTGAAG	5 cut(s)	1050, 1304, 1470, 2010, 2469
Eco88I	CYCGRG	1 cut(s)	1458
EcoNI	CCTNNNNNAGG	2 cut(s)	2377, 2918
EcoO109I	RGGNCCY	3 cut(s)	1167, 1214, 3115
EcoRI	GAATTC	1 cut(s)	1947
EcoRII	CCWGG	5 cut(s)	228, 520, 1196, 2796, 3024
EcoRV	GATATC	1 cut(s)	2391
EcoT14I	CCWWGG	5 cut(s)	950, 1098, 1393, 2836, 3183
EcoT38I	GRGCYC	2 cut(s)	617, 2457
ErhI	CCWWGG	5 cut(s)	950, 1098, 1393, 2836, 3183
FalI	AAGNNNNNCTT	2 cut(s)	1082, 1114
FaqI	GGGAC	4 cut(s)	724, 1180, 1267, 2757
FauI	CCCGC	3 cut(s)	165, 700, 1082
FauNDI	CATATG	1 cut(s)	786
FbaI	TGATCA	1 cut(s)	2080
FblI	GTMKAC	1 cut(s)	2663
FriOI	GRGCYC	2 cut(s)	617, 2457
FspBI	CTAG	3 cut(s)	951, 1218, 1682
FspI	TGCGCA	1 cut(s)	750
GlaI	GCGC	5 cut(s)	32, 429, 438, 750, 1523
GsuI	CTGGAG	5 cut(s)	218, 257, 452, 543, 1989
HaeII	RGCGCY	3 cut(s)	431, 440, 1525
HaeIII	GGCC	8 cut(s)	47, 464, 764, 1085, 1294, 2372, 2500, 2706
HapII	CCGG	6 cut(s)	48, 53, 83, 659, 2531, 3095
HgaI	GACGC	1 cut(s)	792
HhaI	GCGC	5 cut(s)	33, 430, 439, 751, 1524
Hin1I	GRCGYC	3 cut(s)	62, 149, 803
Hin6I	GCGC	5 cut(s)	31, 428, 437, 749, 1522
HinP1I	GCGC	5 cut(s)	31, 428, 437, 749, 1522
HincII	GTYRAC	2 cut(s)	147, 2956
HindII	GTYRAC	2 cut(s)	147, 2956
HindIII	AAGCTT	4 cut(s)	839, 1109, 2283, 2929
HinfI	GANTC	8 cut(s)	100, 448, 472, 490, 1514, 1787, 2071, 2573
HpaII	CCGG	6 cut(s)	48, 53, 83, 659, 2531, 3095
Hpy166II	GTNNAC	7 cut(s)	130, 147, 385, 981, 1249, 2664, 2956
Hpy8I	GTNNAC	7 cut(s)	130, 147, 385, 981, 1249, 2664, 2956
Hpy99I	CGWCG	4 cut(s)	61, 64, 154, 808
HpyCH4III	ACNGT	6 cut(s)	469, 1144, 1672, 2177, 2641, 2748
HpyCH4IV	ACGT	3 cut(s)	62, 149, 1744
HpyF3I	CTNAG	8 cut(s)	1647, 1734, 1889, 2096, 2130, 2693, 2919, 3053
HpySE526I	ACGT	3 cut(s)	62, 149, 1744
Hsp92I	GRCGYC	3 cut(s)	62, 149, 803
HspAI	GCGC	5 cut(s)	31, 428, 437, 749, 1522
Kpn2I	TCCGGA	1 cut(s)	2530
KpnI	GGTACC	1 cut(s)	1074
Ksp22I	TGATCA	1 cut(s)	2080
KspI	CCGCGG	1 cut(s)	279
LmnI	GCTCC	9 cut(s)	90, 225, 238, 352, 457, 511, 535, 2221, 2970
Lsp1109I	GCAGC	6 cut(s)	1006, 1327, 1424, 1952, 2317, 2444
LweI	GCATC	8 cut(s)	661, 849, 936, 1345, 1463, 1909, 2203, 2423
MaeI	CTAG	3 cut(s)	951, 1218, 1682
MaeII	ACGT	3 cut(s)	62, 149, 1744
MaeIII	GTNAC	2 cut(s)	2488, 2716
MfeI	CAATTG	1 cut(s)	2322
MflI	RGATCY	2 cut(s)	264, 1894
MhlI	GDGCHC	5 cut(s)	617, 983, 2457, 2753, 3051
MlsI	TGGCCA	3 cut(s)	764, 1294, 2706
MluNI	TGGCCA	3 cut(s)	764, 1294, 2706
MmeI	TCCRAC	8 cut(s)	132, 288, 435, 1614, 1864, 2313, 2608, 2760
Mox20I	TGGCCA	3 cut(s)	764, 1294, 2706
MroI	TCCGGA	1 cut(s)	2530
MroXI	GAANNNNTTC	1 cut(s)	119
MscI	TGGCCA	3 cut(s)	764, 1294, 2706
MslI	CAYNNNNRTG	2 cut(s)	123, 895
Msp20I	TGGCCA	3 cut(s)	764, 1294, 2706
MspA1I	CMGCKG	3 cut(s)	278, 1943, 1988
MspCI	CTTAAG	4 cut(s)	440, 539, 1418, 3119
MspI	CCGG	6 cut(s)	48, 53, 83, 659, 2531, 3095
MspR9I	CCNGG	5 cut(s)	230, 522, 1198, 2798, 3026
MunI	CAATTG	1 cut(s)	2322
Mva1269I	GAATGC	3 cut(s)	1099, 1404, 2442
MvaI	CCWGG	5 cut(s)	230, 522, 1198, 2798, 3026
MvnI	CGCG	3 cut(s)	33, 278, 389
NcoI	CCATGG	2 cut(s)	1393, 2836
NdeI	CATATG	1 cut(s)	786
NmeAIII	GCCGAG	1 cut(s)	1550
NmuCI	GTSAC	1 cut(s)	2488
NsbI	TGCGCA	1 cut(s)	750
OliI	CACNNNNGTG	1 cut(s)	123
PaeR7I	CTCGAG	1 cut(s)	1458
PagI	TCATGA	2 cut(s)	1285, 2026
PaqCI	CACCTGC	1 cut(s)	2512
PceI	AGGCCT	1 cut(s)	2372
PcsI	WCGNNNNNNNCGW	1 cut(s)	30
PctI	GAATGC	3 cut(s)	1099, 1404, 2442
PdmI	GAANNNNTTC	1 cut(s)	119
PfeI	GAWTC	8 cut(s)	100, 448, 472, 490, 1514, 1787, 2071, 2573
PflFI	GACNNNGTC	1 cut(s)	61
PflMI	CCANNNNNTGG	1 cut(s)	1274
PfoI	TCCNGGA	1 cut(s)	520
PpuMI	RGGWCCY	3 cut(s)	1167, 1214, 3115
Psp5II	RGGWCCY	3 cut(s)	1167, 1214, 3115
Psp6I	CCWGG	5 cut(s)	228, 520, 1196, 2796, 3024
PspGI	CCWGG	5 cut(s)	228, 520, 1196, 2796, 3024
PspPI	GGNCC	6 cut(s)	50, 1084, 1167, 1214, 2498, 3115
PspPPI	RGGWCCY	3 cut(s)	1167, 1214, 3115
PstI	CTGCAG	1 cut(s)	1045
PsuI	RGATCY	2 cut(s)	264, 1894
PsyI	GACNNNGTC	1 cut(s)	61
PvuII	CAGCTG	1 cut(s)	1943
RsaI	GTAC	6 cut(s)	917, 1072, 1138, 1645, 1677, 2023
RsaNI	GTAC	6 cut(s)	916, 1071, 1137, 1644, 1676, 2022
RseI	CAYNNNNRTG	2 cut(s)	123, 895
Rsr2I	CGGWCCG	1 cut(s)	50
RsrII	CGGWCCG	1 cut(s)	50
SacII	CCGCGG	1 cut(s)	279
Sau96I	GGNCC	6 cut(s)	50, 1084, 1167, 1214, 2498, 3115
ScaI	AGTACT	2 cut(s)	917, 1645
ScrFI	CCNGG	5 cut(s)	230, 522, 1198, 2798, 3026
SduI	GDGCHC	5 cut(s)	617, 983, 2457, 2753, 3051
SfaNI	GCATC	8 cut(s)	661, 849, 936, 1345, 1463, 1909, 2203, 2423
SfcI	CTRYAG	2 cut(s)	1041, 1620
Sfr274I	CTCGAG	1 cut(s)	1458
Sfr303I	CCGCGG	1 cut(s)	279
SgrBI	CCGCGG	1 cut(s)	279
SinI	GGWCC	4 cut(s)	50, 1167, 1214, 3115
SlaI	CTCGAG	1 cut(s)	1458
SmiMI	CAYNNNNRTG	2 cut(s)	123, 895
SmlI	CTYRAG	7 cut(s)	440, 539, 1418, 1458, 1538, 1693, 3119
SmoI	CTYRAG	7 cut(s)	440, 539, 1418, 1458, 1538, 1693, 3119
SseBI	AGGCCT	1 cut(s)	2372
SspI	AATATT	2 cut(s)	2401, 3036
SspMI	CTAG	3 cut(s)	951, 1218, 1682
StuI	AGGCCT	1 cut(s)	2372
StyD4I	CCNGG	5 cut(s)	228, 520, 1196, 2796, 3024
StyI	CCWWGG	5 cut(s)	950, 1098, 1393, 2836, 3183
TaaI	ACNGT	6 cut(s)	469, 1144, 1672, 2177, 2641, 2748
TaiI	ACGT	3 cut(s)	65, 152, 1747
TaqI	TCGA	8 cut(s)	218, 593, 993, 1459, 1850, 1926, 2889, 3108
TatI	WGTACW	2 cut(s)	915, 1643
TauI	GCSGC	6 cut(s)	47, 172, 278, 281, 327, 2844
TfiI	GAWTC	8 cut(s)	100, 448, 472, 490, 1514, 1787, 2071, 2573
TscAI	CASTG	8 cut(s)	472, 1008, 1123, 1511, 2497, 2590, 2701, 2753
TseFI	GTSAC	1 cut(s)	2488
TseI	GCWGC	6 cut(s)	1019, 1340, 1412, 1940, 2330, 2432
Tsp45I	GTSAC	1 cut(s)	2488
TspGWI	ACGGA	3 cut(s)	816, 1249, 1318
TspRI	CASTG	8 cut(s)	472, 1008, 1123, 1511, 2497, 2590, 2701, 2753
Tth111I	GACNNNGTC	1 cut(s)	61
Van91I	CCANNNNNTGG	1 cut(s)	1274
Vha464I	CTTAAG	4 cut(s)	440, 539, 1418, 3119
VneI	GTGCAC	1 cut(s)	979
VpaK11BI	GGWCC	4 cut(s)	50, 1167, 1214, 3115
XagI	CCTNNNNNAGG	2 cut(s)	2377, 2918
XapI	RAATTY	6 cut(s)	496, 792, 1281, 1597, 1947, 2063
XhoI	CTCGAG	1 cut(s)	1458
XmaJI	CCTAGG	1 cut(s)	950
XmiI	GTMKAC	1 cut(s)	2663
XmnI	GAANNNNTTC	1 cut(s)	119
XspI	CTAG	3 cut(s)	951, 1218, 1682
ZraI	GACGTC	2 cut(s)	63, 150
ZrmI	AGTACT	2 cut(s)	917, 1645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.