Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001873

Physical Location & Seq

Genomic Coordinates Reverse (-)

20702444 .. 20707324

Gene Structure 4881 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1132100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3192 bp

ATGGGGAAGGGAGGGCAGGATTTGGGTAGACGGAAGGAGGACGGAAACCCAAAGCTGGTGGACGGCGGTGTATTTCCGGCGTGGGCGAAGGAGATTTCGGAGTGCGAGAAGCACTTTGGGGTTAACAGAAAATTTGGGTTGGGATCCAAGGACGTTGAGAAGCGGCGGGAGAAGTACGGCTGGAACGAGTTGGAGAAGAACGAGGGGCAGTCGATTTGGAGTCTGGTTCTGGACCAGTTCAATGATACGCTGGTTCGAATCCTGCTTGCGGCGGCGGTCGTTTCTTTCGTTCTGGCTTGGCTGGACGGGAAGGAAGGCAATAAGAAGGAGATCACGGCGTTTGTGGAGCCGCTGGTGATCTTCTTGATCTTGGTTGTGAACGCAATTGTTGGTGTTTGGCAGGAGAGCGATGCCGAGAAGGCGTTGGAGGCGCTGAAGGAGATTCAATCAGAGCAGGCGACTGTGATTCGGGATGGGAGCAAGGTTCCGAAAATGGCAGCCAAGGAGCTTGTTCCTGGTGACATTGTGGAACTTAAGGTTGGGGATAAAGTGTCCGCTGATATGCGTGTTGTGGAATTGATTAGCTCGACTTTGAGGGTCGAGCAGGGTTCATTGACTGGAGAGAGTGAGGCGGTGAATAAGACTAACAAGCCGGTGTCTGAGGACGCGGACATTCAGGGGAAGAAAAGTATGGTTTTCGCAGGGACAACTATTGTGAATGGAAATTGCATATGCTTGGTTGCACAGACAGGAATGTCCACTGAAATCGGGAAGGTTCATATGCAAATCCATGTTGCCTCGCAGAGCGAAGAAGATACCCCACTTAAGAAGAAGCTCAATGAGTTTGGAGAGATGCTCACGATGGTAATTGGAGTTATTTGTGTATTAGTGTGGCTCATCAATTTTAAGTACTTCCTTACTTGGGAATATGTCAATGGACGGCCAGCGAATTTCAAGTTTTCGTTTGAGAAGTGCACATATTACTTTCAGATCGCAGTAGCGTTGGCTGTGGCTGCTATTCCTGAAGGTCTGCCTGCAGTCATTACGACATGCTTGGCGCTTGGTACCCGCAAAATGGCTCAGAAGAATGCTTTGGTCCGAAAGCTGCCTAGTGTTGAAACTCTGGGTTGTACAACTGTGATTTGCTCAGATAAAACAGGGACATTGACTACAAACCAGATGGCAGTGGCAAAACTTGTAGCTTTGGGTCCTATACCTACTATACTACGGAAGTTTAGGGTGGATGGAACTACATACAATCCACTTGATGGTAAAATTCACGACTGGCCAACTGGTCGTATGGATGCTAATCTTCAAATGATCGCAAAGGTAGCTGCTGTCTGCAATGATGCTGGCATTACACATGCAGAACAGAAGTATATTGCCAATGGAATGCCTACTGAGGCAGCTCTAAAGGTTCTAGTTGAGAAGATGGGCCTTCCCGAAGAGTCCAAGGGTGCGGAATCAACAAATGAAAGGGATTTTCTTGGTTGTTGCCAGAAGTGGAATGAATTTGAATGCCGAATTGCAACCCTTGAGTTTGATCGTGATAGGAAATCCATGGGTGTAATTGTCAATTCCCGTTCAGGGAAGAAGTCATTGCTAGTGAAGGGCGCTGTAGAGAATTTGCTGGAGAGAAGCACTAAGGTTCAATTGCTTGATGGCACTGTTGTACCACTGGATGATAGTTCAAGGAACTATATTGTGGAAGCTCTTCATGAGATGTCAACTATTGCATTACGCTGCTTGGGTTTTGCATACAAAGATGAGCTTGGTGAGTTTGAATCCTACGATGGTGATGAAGATCATCCAGCTCATAGGCTTTTACTTGATCCATCCAATTATTCATCAATTGAGCGCGACCTTGTTTTTGTCGGCCTTGTTGGACTGAGGGATCCCCCAAGAGAGGAGGTTTTTGATGCAATTGAAGACTGTCGAGCAGCTGGAATCCGTGTTATTGTAATCACCGGAGATCACCAGAACACAGCAGAAGCAATATGCCGTGAAATTGGCGTGTTTGGTGCTGATGAAGACATACGTTCAAGAAGCCTTACAGGAAGAGGGTTTATGGGTCTACATGATCAGAAAGCTCATCTTAGACAAGGTGGTGGCCTTCTGTTTTCAAGGGCCGAACCAACGCACAAACAAGAGATTGTGAGGTTACTCAAAGAGGATGGTGAAGTGGTTGCTATGACTGGTGATGGAGTGAATGATGCACCTGCTTTGAAACTTGCTGATATCGGGATTGCAATGGGCATTTCTGGAACTGAGGTTGCAAAGGAAGCTTCTGACATGGTTTTGGCTGATGATAATTTTAGCACAATTGTTGTAGCTATTGGTGAAGGCAGATCTATCTACAATAACATGAAAGCCTTTATCAGGTACATGATCTCATCCAACATTGGTGAGGTTGCCTCCATATTCTTAACAGCAGCATTAGGCATTCCTGAAGGCCTCATACCTGTTCACCTCTTGTGGGTGAATCTTGTTACCGATGGACCACCTGCAACAGCACTAGGATTTAATCCTCCAGACAAGGACATAATGAAGAAACCCCCACGCAGAAGCGACGATTCACTTATCAGCGCTTGGATATTATTCCGCTATCTGGTTATTGGTATGTATGTTGGACTGGCAACTGTAGGCGTATTCGTCATTTGGTATACACATGGTTCCTTCCTAGGTATTGACCTCAGTGGGGACGGCCACTCCCTTGTGACGTACTCCCAACTTGCCAACTGGGGACAATGCTCATCTTGGACAAATTTCACCGCGTCACCCTTCACAGCCGGAAATCAGGTCATGTCTTTTGATGACCCCTGTGATTATTTCCGTGCAGGCAAAGTGAAAGCTATGACACTCTCCCTCTCCGTGTTGGTTGCCATCGAAATGTTCAACTCTGTCAATGCCCTTTCCGAGGACGGAAGCCTGCTGAGCATGCCCCCATGGGTCAACCCCTGGCTCCTTGTGGCCATGTGCGTCTCATTTGGCCTGCACTTCGTGGTCCTGTATGTGCCATTCTTGGCTCAAGTATTTGGCATTGTACCCCTGAGCTTAAACGAATGGCTGCTGGTTTTGGCCGTTGCACAACCCGTGATTCTCATTGATGAGGTCCTCAAGTTTGTCGGAAGATGGGCAAGCGCTTCTCAAATATCCAGGAGGAGAAAACCATCCAAGCCCAAGACAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010042 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1064

Amino Acids

116.4

Weight (kDa)

5.5

Isoelectric Point (pI)

34.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	28 - 95	7.2e-19	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	148 - 260	5e-23	P-type ATPase actuator domain
Cation_ATPase	PF13246	438 - 637	1.7e-38	P-type ATPase, cytoplasmic domain N
HAD	PF12710	616 - 743	1.6e-06	haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	625 - 746	1.8e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	818 - 1042	2.1e-47	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07670 AT1G07670 AT1G07810
fragaria_vesca	FvH4_3g34570 FvH4_3g34570 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760
malus_domestica	MD04G1038900.v1.1 MD04G1116400.v1.1 MD11G1117700.v1.1 MD12G1132100.v1.1 MD13G1142700.v1.1
prunus_persica	Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1
pyrus_communis	pycom03g12390 pycom03g12400 pycom04g10640 pycom07g10780 pycom11g09870 pycom12g12810 pycom16g18850
rosa_chinensis	RchiOBHm_Chr3g0467981 RchiOBHm_Chr5g0062231
rosa_laevigata	RLG00000024462 RLG00000035545
rosa_multiflora	Rmu_co8021382.1_g000001 Rmu_sc0001793.1_g000060 Rmu_ssc0000409.1_g000035
rosa_roxburghii	Rroxscaffold_1G00018460 Rroxscaffold_6G00413240
rosa_rugosa	Rorug03G0090700 Rorug03G0090800 Rorug05G0347300
rosa_samantha	Rh3AG139600 Rh3BG161100 Rh3CG161000 Rh3DG161500 Rh5AG407900 Rh5BG421600 Rh5CG445600 Rh5DG436600
rosa_wichuraiana	Rw3G013200 Rw5G038440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	2227, 2512
AasI	GACNNNNNNGTC	1 cut(s)	754
Acc36I	ACCTGC	2 cut(s)	2227, 2512
Acc65I	GGTACC	1 cut(s)	1064
AccB1I	GGYRCC	1 cut(s)	1064
AccB7I	CCANNNNNTGG	1 cut(s)	1268
AccI	GTMKAC	3 cut(s)	28, 2074, 2663
AccII	CGCG	3 cut(s)	668, 1860, 2774
AclWI	GGATC	5 cut(s)	138, 151, 1826, 1889, 1902
AcoI	YGGCCR	5 cut(s)	941, 1286, 2704, 2970, 3078
AcsI	RAATTY	6 cut(s)	131, 949, 1275, 1511, 1624, 2764
AcuI	CTGAAG	3 cut(s)	455, 1044, 2469
AdeI	CACNNNGTG	1 cut(s)	3001
AfaI	GTAC	8 cut(s)	176, 911, 1066, 1132, 1674, 2384, 2723, 3045
AfeI	AGCGCT	2 cut(s)	2587, 3142
AflII	CTTAAG	2 cut(s)	533, 824
AjnI	CCWGG	3 cut(s)	514, 2957, 3155
AjuI	GAANNNNNNNTTGG	4 cut(s)	522, 554, 1076, 1108
Alw21I	GWGCWC	1 cut(s)	977
Alw26I	GTCTC	1 cut(s)	2986
Alw44I	GTGCAC	1 cut(s)	973
AlwI	GGATC	5 cut(s)	138, 151, 1826, 1889, 1902
Aor51HI	AGCGCT	2 cut(s)	2587, 3142
ApaLI	GTGCAC	1 cut(s)	973
ApeKI	GCWGC	9 cut(s)	497, 1013, 1105, 1334, 1406, 1743, 1940, 2432, 3067
ApoI	RAATTY	6 cut(s)	131, 949, 1275, 1511, 1624, 2764
ArsI	GACNNNNNNTTYG	2 cut(s)	1853, 1885
Asp700I	GAANNNNTTC	1 cut(s)	1713
Asp718I	GGTACC	1 cut(s)	1064
AspA2I	CCTAGG	1 cut(s)	2680
AspLEI	GCGC	6 cut(s)	433, 1060, 1616, 1860, 2588, 3143
AspS9I	GGNCC	8 cut(s)	232, 1096, 1208, 1435, 2127, 2498, 3004, 3112
AsuII	TTCGAA	1 cut(s)	256
AvaII	GGWCC	6 cut(s)	232, 1096, 1208, 2498, 3004, 3112
AvrII	CCTAGG	1 cut(s)	2680
BaeGI	GKGCMC	1 cut(s)	977
BaeI	ACNNNNGTAYC	4 cut(s)	237, 270, 1656, 1689
BalI	TGGCCA	2 cut(s)	1288, 2972
BamHI	GGATCC	2 cut(s)	143, 1894
BanI	GGYRCC	1 cut(s)	1064
BarI	GAAGNNNNNNTAC	2 cut(s)	2442, 2474
BbsI	GAAGAC	2 cut(s)	1935, 2037
Bbv12I	GWGCWC	1 cut(s)	977
BbvI	GCAGC	9 cut(s)	509, 1000, 1092, 1321, 1418, 1730, 1952, 2444, 3054
BceAI	ACGGC	7 cut(s)	79, 193, 351, 956, 1986, 2719, 3065
BciT130I	CCWGG	3 cut(s)	516, 2959, 3157
BclI	TGATCA	1 cut(s)	2080
BcoDI	GTCTC	1 cut(s)	2986
BfaI	CTAG	5 cut(s)	1110, 1421, 1604, 2516, 2681
BfmI	CTRYAG	3 cut(s)	1035, 1617, 2640
BfoI	RGCGCY	5 cut(s)	434, 1061, 1617, 2589, 3144
BfrI	CTTAAG	2 cut(s)	533, 824
BfuAI	ACCTGC	2 cut(s)	2227, 2512
BglI	GCCNNNNNGGC	1 cut(s)	419
BglII	AGATCT	1 cut(s)	2348
BlnI	CCTAGG	1 cut(s)	2680
BlpI	GCTNAGC	1 cut(s)	2933
BmcAI	AGTACT	1 cut(s)	911
Bme1390I	CCNGG	3 cut(s)	516, 2959, 3157
Bme18I	GGWCC	6 cut(s)	232, 1096, 1208, 2498, 3004, 3112
BmgT120I	GGNCC	8 cut(s)	232, 1096, 1208, 1435, 2127, 2498, 3004, 3112
BmiI	GGNNCC	8 cut(s)	145, 348, 486, 1066, 1209, 1896, 2674, 2963
BmrFI	CCNGG	3 cut(s)	516, 2959, 3157
BmrI	ACTGGG	1 cut(s)	2749
BmsI	GCATC	6 cut(s)	400, 843, 1294, 1339, 1909, 2203
BmuI	ACTGGG	1 cut(s)	2749
BpiI	GAAGAC	2 cut(s)	1935, 2037
BplI	GAGNNNNNCTC	4 cut(s)	840, 872, 2399, 2431
BpmI	CTGGAG	3 cut(s)	639, 1652, 2514
Bpu10I	CCTNAGC	1 cut(s)	3050
Bpu1102I	GCTNAGC	1 cut(s)	2933
Bpu14I	TTCGAA	1 cut(s)	256
BpuEI	CTTGAG	3 cut(s)	1556, 3012, 3101
BsaBI	GATNNNNATC	2 cut(s)	1308, 1803
BsaJI	CCNNGG	8 cut(s)	147, 501, 1452, 1560, 2680, 2916, 2945, 2957
BsaWI	WCCGGW	1 cut(s)	1967
BsaXI	ACNNNNNCTCC	2 cut(s)	2693, 2723
Bse118I	RCCGGY	1 cut(s)	652
Bse1I	ACTGG	8 cut(s)	235, 622, 1289, 1297, 1683, 2200, 2637, 2744
Bse3DI	GCAATG	3 cut(s)	1351, 1598, 2256
Bse8I	GATNNNNATC	2 cut(s)	1308, 1803
BseBI	CCWGG	3 cut(s)	516, 2959, 3157
BseDI	CCNNGG	8 cut(s)	147, 501, 1452, 1560, 2680, 2916, 2945, 2957
BseGI	GGATG	9 cut(s)	478, 1249, 1309, 1687, 1807, 1835, 2179, 2393, 3170
BseJI	GATNNNNATC	2 cut(s)	1308, 1803
BseMI	GCAATG	3 cut(s)	1351, 1598, 2256
BseMII	CTCAG	9 cut(s)	651, 1094, 1161, 1392, 1880, 2259, 2707, 2924, 3041
BseNI	ACTGG	8 cut(s)	235, 622, 1289, 1297, 1683, 2200, 2637, 2744
BseRI	GAGGAG	2 cut(s)	1922, 3175
BseSI	GKGCMC	1 cut(s)	977
BseXI	GCAGC	9 cut(s)	509, 1000, 1092, 1321, 1418, 1730, 1952, 2444, 3054
BsgI	GTGCAG	2 cut(s)	2856, 2978
Bsh1236I	CGCG	3 cut(s)	668, 1860, 2774
Bsh1285I	CGRYCG	1 cut(s)	279
BshNI	GGYRCC	1 cut(s)	1064
BsiEI	CGRYCG	1 cut(s)	279
BsiHKAI	GWGCWC	1 cut(s)	977
BsiSI	CCGG	4 cut(s)	77, 653, 1968, 2790
BslFI	GGGAC	4 cut(s)	718, 1174, 2714, 2757
BsmAI	GTCTC	1 cut(s)	2986
BsmBI	CGTCTC	1 cut(s)	2986
BsmFI	GGGAC	4 cut(s)	718, 1174, 2714, 2757
BsmI	GAATGC	4 cut(s)	1093, 1398, 1523, 2442
Bsp119I	TTCGAA	1 cut(s)	256
Bsp1286I	GDGCHC	1 cut(s)	977
Bsp1407I	TGTACA	1 cut(s)	1130
Bsp1720I	GCTNAGC	1 cut(s)	2933
Bsp19I	CCATGG	2 cut(s)	1560, 2945
BspCNI	CTCAG	9 cut(s)	652, 1093, 1160, 1393, 1881, 2260, 2706, 2925, 3042
BspFNI	CGCG	3 cut(s)	668, 1860, 2774
BspHI	TCATGA	1 cut(s)	1717
BspLI	GGNNCC	8 cut(s)	145, 348, 486, 1066, 1209, 1896, 2674, 2963
BspMAI	CTGCAG	1 cut(s)	1039
BspMI	ACCTGC	2 cut(s)	2227, 2512
BspPI	GGATC	5 cut(s)	138, 151, 1826, 1889, 1902
BspQI	GCTCTTC	1 cut(s)	1719
BspT104I	TTCGAA	1 cut(s)	256
BspT107I	GGYRCC	1 cut(s)	1064
BspTI	CTTAAG	2 cut(s)	533, 824
BsrDI	GCAATG	3 cut(s)	1351, 1598, 2256
BsrFI	RCCGGY	1 cut(s)	652
BsrGI	TGTACA	1 cut(s)	1130
BsrI	ACTGG	8 cut(s)	235, 622, 1289, 1297, 1683, 2200, 2637, 2744
BssAI	RCCGGY	1 cut(s)	652
BssECI	CCNNGG	8 cut(s)	147, 501, 1452, 1560, 2680, 2916, 2945, 2957
BssNAI	GTATAC	1 cut(s)	2664
BssT1I	CCWWGG	6 cut(s)	147, 501, 1452, 1560, 2680, 2945
Bst1107I	GTATAC	1 cut(s)	2664
Bst2UI	CCWGG	3 cut(s)	516, 2959, 3157
Bst4CI	ACNGT	5 cut(s)	463, 1138, 1669, 1934, 2641
Bst6I	CTCTTC	3 cut(s)	1440, 1719, 2053
BstAFI	CTTAAG	2 cut(s)	533, 824
BstAUI	TGTACA	1 cut(s)	1130
BstBI	TTCGAA	1 cut(s)	256
BstDSI	CCRYGG	2 cut(s)	1560, 2945
BstENI	CCTNNNNNAGG	2 cut(s)	2377, 2915
BstF5I	GGATG	9 cut(s)	478, 1249, 1309, 1687, 1807, 1835, 2179, 2393, 3170
BstFNI	CGCG	3 cut(s)	668, 1860, 2774
BstH2I	RGCGCY	5 cut(s)	434, 1061, 1617, 2589, 3144
BstHHI	GCGC	6 cut(s)	433, 1060, 1616, 1860, 2588, 3143
BstMAI	GTCTC	1 cut(s)	2986
BstMCI	CGRYCG	1 cut(s)	279
BstMWI	GCNNNNNNNGC	7 cut(s)	419, 428, 1013, 1331, 2282, 2934, 2938
BstNI	CCWGG	3 cut(s)	516, 2959, 3157
BstNSI	RCATGY	3 cut(s)	1053, 1367, 2941
BstSCI	CCNGG	3 cut(s)	514, 2957, 3155
BstSFI	CTRYAG	3 cut(s)	1035, 1617, 2640
BstSLI	GKGCMC	1 cut(s)	977
BstUI	CGCG	3 cut(s)	668, 1860, 2774
BstV1I	GCAGC	9 cut(s)	509, 1000, 1092, 1321, 1418, 1730, 1952, 2444, 3054
BstV2I	GAAGAC	2 cut(s)	1935, 2037
BstX2I	RGATCY	3 cut(s)	143, 1894, 2348
BstYI	RGATCY	3 cut(s)	143, 1894, 2348
BstZ17I	GTATAC	1 cut(s)	2664
BtgI	CCRYGG	2 cut(s)	1560, 2945
BtgZI	GCGATG	1 cut(s)	423
BtsCI	GGATG	9 cut(s)	478, 1249, 1309, 1687, 1807, 1835, 2179, 2393, 3170
BtsI	GCAGTG	1 cut(s)	1191
BtsIMutI	CAGTG	5 cut(s)	759, 1191, 1665, 1676, 2701
BveI	ACCTGC	2 cut(s)	2227, 2512
CciI	TCATGA	1 cut(s)	1717
CfoI	GCGC	6 cut(s)	433, 1060, 1616, 1860, 2588, 3143
Cfr10I	RCCGGY	1 cut(s)	652
Cfr13I	GGNCC	8 cut(s)	232, 1096, 1208, 1435, 2127, 2498, 3004, 3112
CseI	GACGC	3 cut(s)	674, 2763, 2968
Csp6I	GTAC	8 cut(s)	175, 910, 1065, 1131, 1673, 2383, 2722, 3044
CspCI	CAANNNNNGTGG	2 cut(s)	39, 74
CviQI	GTAC	8 cut(s)	175, 910, 1065, 1131, 1673, 2383, 2722, 3044
DraIII	CACNNNGTG	1 cut(s)	3001
DrdI	GACNNNNNNGTC	1 cut(s)	754
DseDI	GACNNNNNNGTC	1 cut(s)	754
EaeI	YGGCCR	5 cut(s)	941, 1286, 2704, 2970, 3078
Eam1104I	CTCTTC	3 cut(s)	1440, 1719, 2053
EarI	CTCTTC	3 cut(s)	1440, 1719, 2053
Eco130I	CCWWGG	6 cut(s)	147, 501, 1452, 1560, 2680, 2945
Eco147I	AGGCCT	1 cut(s)	2454
Eco32I	GATATC	1 cut(s)	2239
Eco47I	GGWCC	6 cut(s)	232, 1096, 1208, 2498, 3004, 3112
Eco47III	AGCGCT	2 cut(s)	2587, 3142
Eco57I	CTGAAG	3 cut(s)	455, 1044, 2469
EcoNI	CCTNNNNNAGG	2 cut(s)	2377, 2915
EcoO109I	RGGNCCY	2 cut(s)	1208, 3112
EcoRII	CCWGG	3 cut(s)	514, 2957, 3155
EcoRV	GATATC	1 cut(s)	2239
EcoT14I	CCWWGG	6 cut(s)	147, 501, 1452, 1560, 2680, 2945
ErhI	CCWWGG	6 cut(s)	147, 501, 1452, 1560, 2680, 2945
Esp3I	CGTCTC	1 cut(s)	2986
FalI	AAGNNNNNCTT	4 cut(s)	1755, 1787, 2079, 2111
FaqI	GGGAC	4 cut(s)	718, 1174, 2714, 2757
FauI	CCCGC	2 cut(s)	159, 1076
FauNDI	CATATG	2 cut(s)	731, 780
FbaI	TGATCA	1 cut(s)	2080
FblI	GTMKAC	3 cut(s)	28, 2074, 2663
FokI	GGATG	9 cut(s)	485, 1256, 1316, 1694, 1794, 1822, 2186, 2380, 3157
FspBI	CTAG	5 cut(s)	1110, 1421, 1604, 2516, 2681
GlaI	GCGC	6 cut(s)	432, 1059, 1615, 1859, 2587, 3142
GsuI	CTGGAG	3 cut(s)	639, 1652, 2514
HaeII	RGCGCY	5 cut(s)	434, 1061, 1617, 2589, 3144
HapII	CCGG	4 cut(s)	77, 653, 1968, 2790
HgaI	GACGC	3 cut(s)	674, 2763, 2968
HhaI	GCGC	6 cut(s)	433, 1060, 1616, 1860, 2588, 3143
Hin6I	GCGC	6 cut(s)	431, 1058, 1614, 1858, 2586, 3141
HinP1I	GCGC	6 cut(s)	431, 1058, 1614, 1858, 2586, 3141
HincII	GTYRAC	3 cut(s)	124, 1728, 2953
HindII	GTYRAC	3 cut(s)	124, 1728, 2953
HindIII	AAGCTT	1 cut(s)	2283
HpaI	GTTAAC	1 cut(s)	124
HpaII	CCGG	4 cut(s)	77, 653, 1968, 2790
Hpy99I	CGWCG	1 cut(s)	2573
HpyCH4III	ACNGT	5 cut(s)	463, 1138, 1669, 1934, 2641
HpyCH4IV	ACGT	3 cut(s)	153, 2038, 2720
HpyF10VI	GCNNNNNNNGC	7 cut(s)	419, 428, 1013, 1331, 2282, 2934, 2938
HpySE526I	ACGT	3 cut(s)	153, 2038, 2720
HspAI	GCGC	6 cut(s)	431, 1058, 1614, 1858, 2586, 3141
KpnI	GGTACC	1 cut(s)	1068
Ksp22I	TGATCA	1 cut(s)	2080
KspAI	GTTAAC	1 cut(s)	124
LguI	GCTCTTC	1 cut(s)	1719
LmnI	GCTCC	4 cut(s)	346, 477, 505, 2967
Lsp1109I	GCAGC	9 cut(s)	509, 1000, 1092, 1321, 1418, 1730, 1952, 2444, 3054
LweI	GCATC	6 cut(s)	400, 843, 1294, 1339, 1909, 2203
MaeI	CTAG	5 cut(s)	1110, 1421, 1604, 2516, 2681
MaeII	ACGT	3 cut(s)	153, 2038, 2720
MaeIII	GTNAC	5 cut(s)	518, 2160, 2488, 2716, 2775
MfeI	CAATTG	5 cut(s)	384, 1652, 1851, 1923, 2322
MflI	RGATCY	3 cut(s)	143, 1894, 2348
MhlI	GDGCHC	1 cut(s)	977
MlsI	TGGCCA	2 cut(s)	1288, 2972
MluNI	TGGCCA	2 cut(s)	1288, 2972
MlyI	GAGTC	2 cut(s)	229, 1457
MmeI	TCCRAC	6 cut(s)	171, 405, 1864, 2421, 2608, 3106
Mox20I	TGGCCA	2 cut(s)	1288, 2972
MroXI	GAANNNNTTC	1 cut(s)	1713
MscI	TGGCCA	2 cut(s)	1288, 2972
MseI	TTAA	7 cut(s)	123, 534, 825, 906, 2426, 2523, 3056
MslI	CAYNNNNRTG	1 cut(s)	2888
Msp20I	TGGCCA	2 cut(s)	1288, 2972
MspA1I	CMGCKG	3 cut(s)	352, 557, 1943
MspCI	CTTAAG	2 cut(s)	533, 824
MspI	CCGG	4 cut(s)	77, 653, 1968, 2790
MspR9I	CCNGG	3 cut(s)	516, 2959, 3157
MunI	CAATTG	5 cut(s)	384, 1652, 1851, 1923, 2322
Mva1269I	GAATGC	4 cut(s)	1093, 1398, 1523, 2442
MvaI	CCWGG	3 cut(s)	516, 2959, 3157
MvnI	CGCG	3 cut(s)	668, 1860, 2774
MwoI	GCNNNNNNNGC	7 cut(s)	419, 428, 1013, 1331, 2282, 2934, 2938
NcoI	CCATGG	2 cut(s)	1560, 2945
NdeI	CATATG	2 cut(s)	731, 780
NlaIV	GGNNCC	8 cut(s)	145, 348, 486, 1066, 1209, 1896, 2674, 2963
NmeAIII	GCCGAG	1 cut(s)	439
NmuCI	GTSAC	3 cut(s)	518, 2716, 2775
NspI	RCATGY	3 cut(s)	1053, 1367, 2941
NspV	TTCGAA	1 cut(s)	256
PaeI	GCATGC	1 cut(s)	2941
PagI	TCATGA	1 cut(s)	1717
PaqCI	CACCTGC	2 cut(s)	2227, 2512
PceI	AGGCCT	1 cut(s)	2454
PciSI	GCTCTTC	1 cut(s)	1719
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 285
PctI	GAATGC	4 cut(s)	1093, 1398, 1523, 2442
PdmI	GAANNNNTTC	1 cut(s)	1713
PfeI	GAWTC	9 cut(s)	258, 442, 466, 1463, 1784, 1947, 2482, 2573, 3097
PflMI	CCANNNNNTGG	1 cut(s)	1268
PfoI	TCCNGGA	1 cut(s)	3155
PleI	GAGTC	2 cut(s)	228, 1456
PpsI	GAGTC	2 cut(s)	228, 1456
PpuMI	RGGWCCY	2 cut(s)	1208, 3112
Psp5II	RGGWCCY	2 cut(s)	1208, 3112
Psp6I	CCWGG	3 cut(s)	514, 2957, 3155
PspGI	CCWGG	3 cut(s)	514, 2957, 3155
PspN4I	GGNNCC	8 cut(s)	145, 348, 486, 1066, 1209, 1896, 2674, 2963
PspPI	GGNCC	8 cut(s)	232, 1096, 1208, 1435, 2127, 2498, 3004, 3112
PspPPI	RGGWCCY	2 cut(s)	1208, 3112
PstI	CTGCAG	1 cut(s)	1039
PsuI	RGATCY	3 cut(s)	143, 1894, 2348
PvuII	CAGCTG	1 cut(s)	1943
RsaI	GTAC	8 cut(s)	176, 911, 1066, 1132, 1674, 2384, 2723, 3045
RsaNI	GTAC	8 cut(s)	175, 910, 1065, 1131, 1673, 2383, 2722, 3044
RseI	CAYNNNNRTG	1 cut(s)	2888
SapI	GCTCTTC	1 cut(s)	1719
SaqAI	TTAA	7 cut(s)	123, 534, 825, 906, 2426, 2523, 3056
Sau96I	GGNCC	8 cut(s)	232, 1096, 1208, 1435, 2127, 2498, 3004, 3112
ScaI	AGTACT	1 cut(s)	911
SchI	GAGTC	2 cut(s)	229, 1457
ScrFI	CCNGG	3 cut(s)	516, 2959, 3157
SduI	GDGCHC	1 cut(s)	977
SfaNI	GCATC	6 cut(s)	400, 843, 1294, 1339, 1909, 2203
SfcI	CTRYAG	3 cut(s)	1035, 1617, 2640
SfuI	TTCGAA	1 cut(s)	256
SinI	GGWCC	6 cut(s)	232, 1096, 1208, 2498, 3004, 3112
SmiMI	CAYNNNNRTG	1 cut(s)	2888
SmlI	CTYRAG	5 cut(s)	533, 824, 1535, 3027, 3116
SmoI	CTYRAG	5 cut(s)	533, 824, 1535, 3027, 3116
SphI	GCATGC	1 cut(s)	2941
SseBI	AGGCCT	1 cut(s)	2454
SspMI	CTAG	5 cut(s)	1110, 1421, 1604, 2516, 2681
StuI	AGGCCT	1 cut(s)	2454
StyD4I	CCNGG	3 cut(s)	514, 2957, 3155
StyI	CCWWGG	6 cut(s)	147, 501, 1452, 1560, 2680, 2945
TaaI	ACNGT	5 cut(s)	463, 1138, 1669, 1934, 2641
TaiI	ACGT	3 cut(s)	156, 2041, 2723
TaqI	TCGA	6 cut(s)	212, 256, 587, 600, 1936, 2886
TatI	WGTACW	2 cut(s)	909, 1130
TauI	GCSGC	4 cut(s)	166, 272, 275, 352
TfiI	GAWTC	9 cut(s)	258, 442, 466, 1463, 1784, 1947, 2482, 2573, 3097
Tru1I	TTAA	7 cut(s)	123, 534, 825, 906, 2426, 2523, 3056
Tru9I	TTAA	7 cut(s)	123, 534, 825, 906, 2426, 2523, 3056
TscAI	CASTG	5 cut(s)	766, 1191, 1672, 1683, 2701
TseFI	GTSAC	3 cut(s)	518, 2716, 2775
TseI	GCWGC	9 cut(s)	497, 1013, 1105, 1334, 1406, 1743, 1940, 2432, 3067
Tsp45I	GTSAC	3 cut(s)	518, 2716, 2775
TspGWI	ACGGA	7 cut(s)	46, 57, 1243, 1940, 2822, 2860, 2937
TspRI	CASTG	5 cut(s)	766, 1191, 1672, 1683, 2701
Van91I	CCANNNNNTGG	1 cut(s)	1268
Vha464I	CTTAAG	2 cut(s)	533, 824
VneI	GTGCAC	1 cut(s)	973
VpaK11BI	GGWCC	6 cut(s)	232, 1096, 1208, 2498, 3004, 3112
XagI	CCTNNNNNAGG	2 cut(s)	2377, 2915
XapI	RAATTY	6 cut(s)	131, 949, 1275, 1511, 1624, 2764
XceI	RCATGY	3 cut(s)	1053, 1367, 2941
XmaJI	CCTAGG	1 cut(s)	2680
XmiI	GTMKAC	3 cut(s)	28, 2074, 2663
XmnI	GAANNNNTTC	1 cut(s)	1713
XspI	CTAG	5 cut(s)	1110, 1421, 1604, 2516, 2681
ZrmI	AGTACT	1 cut(s)	911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.