Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0001873

Physical Location & Seq

Genomic Coordinates Reverse (-)

35644774 .. 35650785

Gene Structure 6012 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00413240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3267 bp

ATGGGGAAAGGAGGTGAGGATTTCGGTAAGCGAGAGAAGGCGGCGGGGCCTACGACGTCGTCGGATCGCGATGCGTTTCCGGCGTGGAGTAAGGAGATTCATGAGTGCGAGAAGCACTTCGGTGTGAGCAGGAAATTAGGGCTGACGTCGGCGGAGGTGGCGAAGCGGCGGGAGGAGTACGGGTGGAACGAGTTGGAGAAGCACGAGGGGCAGTCGATTTGGAGTCTGGTGCTCGACCAGTTCAACGATACTCTGGTGCGGATCCTGCTCGCGGCGGCGGTCATCTCGTTTGTGCTGGCGTGGTTGGACGGGGAGGAAGGCGGTGAGAAGGAGATCACGGCGTTTGTGGAGCCGTTGGTGATCTTTTTGATTCTGATTGTGAACGCGATTGTTGGAGTTTGGCAGGAGAGCAATGCCGAGAAGGCGCTGGAGGCGCTTAAGGAGATTCAGTCGGACCAGGCGACTGTGATTCGGAATGGGAGTAAGGTTCCGAATTTGCCGGCGAAGGAGCTCGTTCCCGGAGATATTGTGGAGCTTAAGGTTGGGGATAAGGTTCCGGCTGATATGCGTGTAGTGGAGTTGATTAGCTCGACCTTGAGGGTTGAGCAGGGTTCGTTGACTGGAGAGAGTGAGGCTGTGAATAAGACTAACAAGCCGGTTCCGGAGGATGCTGATATTCAGGGGAAGTGGTCTATGGTGTTTGCCGGGACGACTATTGTGAATGGGAATTGCATTTGCTTGGTTGCGCAGACCGGAATGGCCACCGAGATTGGGAAGGTGCATACGCAAATTCATGTGGCTTCACAGAGTGAGGAAGACACTCCGTTGAAGAAGAAGCTTAATGAGTTTGGGGAGATGCTCACTATGATAATTGGAGTGATTTGTGCATTGGTGTGGCTCATCAATGTCAAGTACTTCCTCACTTGGGAATATGTTGATGGGATGCCGAGTAACTTCAAGTTTTCATTTGAGAAGTGCACTTATTACTTCGAGATTGCAGTGGCACTGGCTGTGGCTGCTATTCCTGAAGGTTTGCCAGCAGTCATCACTACCTGCTTGGCACTTGGTACCCGCAAAATGGCTCAGAAGAATGCCTTGGTTAGAAAGCTTCCCAGTGTTGAAACTCTGGGTTGTACCACAGTCATTTGTTCTGACAAAACAGGGACCTTGACTACGAACCAAATGGCAGTTTCCAGGCTTGTAGCTTTCGGTCCTAGACCAACTATACTACGGAAGTTTAAGGTGGATGGGACTACTTACAATCCAGCAGATGGCAAAATTTATGGCTGGCCAAGTGGACGTATGGATGCTAATCTTCAAACGATTGCAAAGGTAGCTGCTGTCTGTAATGATGCTGGTATTACCCAATCAGAACAGAAGTATGTTGCCCATGGAATGCCTACCGAGGCAGCTCTTAAGGAGGATCCGTTTGTCAAGTATTTCTATCCTGTGAAAGCAAGTCATTTTGTTAACTCTGGCTCACTGTCATTTCAGGTTTTAGTTGAGAAGATGGGGCTTCCTGAAGCATCTCGAGGTGTGGGATCAAGTAAAACTGATTTACTAGGTTGTTGCAAGCACTGGAATGAATCTGAGCGCCGAGTGGCAACTCTTGAGTTTGATCGTGATAGGAAATCTATGGGTGTCATTGCCACCTCTCGTTCAGGGAAAAATTCTTTGCTAGTGAAGGGTGCTGTAGAAAATGTGTTGGAGAGAAGCACTCAGGTTCAGTTGCTTGATGGAACTGTTGTACCACTAGATAATAACTTGAGGAACTACATCTTACAAGCTCTTAATGAGATGTCATCTGATGCACTACGTTGTCTGGGATTTGCATATAAAGATGACTTGGCTGACTTTGAGTCCTATGATGGTGATGAGCATCCAGCTCACAAGCAATTACTTGATCCATCCAATTATTCATCAATAGAGAGCGAACTTGTTTTTGTTGGCTTGGTTGGACTGAGGGATCCACCAAGAGAGGAGGTTTTTGATGCAATTGAAGATTGCCGTGCAGCGGGAATTCGTGTTATGGTAATTACAGGAGATAACAAGAACACAGCAGAAGCTATATGTCGTGAAATAGGTGTTTTTGGTACTCATGAAGACATAAGTACAAGAAGCATAACAGGAAGAGAGTTTATGAATCTGCCTGATCAGAAAGACTTTCTGAGACAAGGTGGTGGGCTGCTCTTTTCAAGGGCTGAACCAAGGCACAAACAAGAGATTGTGAGGTTGCTGAAAGATGAAGGTGAGGTGGTTGCCATGACTGGTGATGGAGTAAATGATGCACCTGCATTGAAACTTGCTGATATTGGAATTGCAATGGGCATTGCTGGAACCGAGGTTGCAAAGGAAGCTTCCGACATGGTTTTGGCAGATGATAATTTCAGCACAATTGTTGCTGCTGTTGGTGAGGGCAGATCTATCTACAACAACATGAAGGCCTTTATCAGGTACATGATCTCCTCCAATATTGGTGAGGTTGCATCTATATTCCTTACAGCAGCATTGGGCATTCCTGAAGGGCTCATCCCTGTCCAGCTTTTGTGGGTCAATCTTGTCACTGATGGGCCACCTGCAACAGCATTAGGATTTAATCCTCCGGACAAGGACATAATGAAGAAACCCCCACGCAGAAGTGATGATTCACTGATCAGCGCGTGGATATTGTTCCGATATTTGGTAATTGGGTTGTATGTTGGAGTAGCAACAGTCGGCGTATTCATAATTTGGTATACTCATAGTTCCTTCATGGGTATCGACCTCAGTGGGGATGGCCACTCCCTTGTTACTTACTCCCAACTTGCCAACTGGGGACAGTGCTCAACTTGGCAGAACTTTACTGCTTCACCTTTCACAGCTGGAAGTGAGGTTATCTCGTTTGACAAAAACCCATGCGATTATTTCCATGGCGGCAAAGTTAAGGCTATGACCCTCTCCCTCTCTGTGTTGGTTGCCATCGAAATGTTCAACTCGCTCAATGCCCTTTCTGAGGATGGAAGCCTGTTGTCAATGCCTCCATGGGTCAACCCTTGGCTCCTTGTGGCCATGTCTGTTTCATTTGGCCTTCATTTCTTGATCCTGTATGTGCCTTGCCTAGCTCAAATATTCGGCATTGTACCCCTGAGTCTAAACGAATGGCTGTTGGTTTTGGCCGTTTCATTGCCGGTGATTCTCATTGATGAGATCCTCAAGTTAGTCGGCAGATGGACAAGCAAGTCTCATATATCCAGGAGGAGAAAACCATCGAAGCCCAAGGCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010042 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1088

Amino Acids

118.99

Weight (kDa)

5.38

Isoelectric Point (pI)

38.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	29 - 96	8.2e-21	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	149 - 263	1.4e-23	P-type ATPase actuator domain
Cation_ATPase	PF13246	439 - 661	9.6e-37	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	648 - 770	3.4e-15	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	842 - 1067	1.8e-49	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07670 AT1G07670 AT1G07810
fragaria_vesca	FvH4_3g34570 FvH4_3g34570 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760
malus_domestica	MD04G1038900.v1.1 MD04G1116400.v1.1 MD11G1117700.v1.1 MD12G1132100.v1.1 MD13G1142700.v1.1
prunus_persica	Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1
pyrus_communis	pycom03g12390 pycom03g12400 pycom04g10640 pycom07g10780 pycom11g09870 pycom12g12810 pycom16g18850
rosa_chinensis	RchiOBHm_Chr3g0467981 RchiOBHm_Chr5g0062231
rosa_laevigata	RLG00000024462 RLG00000035545
rosa_multiflora	Rmu_co8021382.1_g000001 Rmu_sc0001793.1_g000060 Rmu_ssc0000409.1_g000035
rosa_roxburghii	Rroxscaffold_1G00018460 Rroxscaffold_6G00413240
rosa_rugosa	Rorug03G0090700 Rorug03G0090800 Rorug05G0347300
rosa_samantha	Rh3AG139600 Rh3BG161100 Rh3CG161000 Rh3DG161500 Rh5AG407900 Rh5BG421600 Rh5CG445600 Rh5DG436600
rosa_wichuraiana	Rw3G013200 Rw5G038440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	2299, 2584
AasI	GACNNNNNNGTC	1 cut(s)	3217
AatII	GACGTC	2 cut(s)	59, 149
Acc16I	TGCGCA	1 cut(s)	747
Acc36I	ACCTGC	3 cut(s)	1061, 2299, 2584
Acc65I	GGTACC	1 cut(s)	1067
AccB1I	GGYRCC	1 cut(s)	1067
AccB7I	CCANNNNNTGG	1 cut(s)	1271
AccI	GTMKAC	1 cut(s)	2735
AccII	CGCG	4 cut(s)	69, 272, 386, 2660
AccIII	TCCGGA	2 cut(s)	661, 2602
AcoI	YGGCCR	5 cut(s)	759, 1289, 2776, 3045, 3153
AcsI	RAATTY	5 cut(s)	493, 789, 1278, 1669, 2019
AcuI	CTGAAG	3 cut(s)	1047, 1542, 2541
AcyI	GRCGYC	2 cut(s)	56, 146
AdeI	CACNNNGTG	1 cut(s)	809
AfaI	GTAC	9 cut(s)	179, 914, 1069, 1135, 1749, 2095, 2113, 2456, 3120
AflII	CTTAAG	3 cut(s)	437, 536, 1415
AgsI	TTSAA	9 cut(s)	244, 829, 958, 1121, 1319, 2000, 2196, 2299, 2971
AhdI	GACNNNNNGTC	1 cut(s)	1858
AjnI	CCWGG	3 cut(s)	456, 1193, 3230
AjuI	GAANNNNNNNTTGG	2 cut(s)	1079, 1111
AleI	CACNNNNGTG	1 cut(s)	120
Alw21I	GWGCWC	4 cut(s)	234, 513, 980, 2825
Alw26I	GTCTC	2 cut(s)	2164, 3225
Alw44I	GTGCAC	1 cut(s)	976
Ama87I	CYCGRG	1 cut(s)	1530
Aor13HI	TCCGGA	2 cut(s)	661, 2602
AoxI	GGCC	9 cut(s)	47, 759, 1289, 2442, 2570, 2776, 3045, 3064, 3153
ApaLI	GTGCAC	1 cut(s)	976
ApeKI	GCWGC	7 cut(s)	1016, 1337, 1409, 2012, 2185, 2402, 2504
ApoI	RAATTY	5 cut(s)	493, 789, 1278, 1669, 2019
Asp700I	GAANNNNTTC	1 cut(s)	116
Asp718I	GGTACC	1 cut(s)	1067
AspLEI	GCGC	5 cut(s)	427, 436, 748, 1596, 2660
AspS9I	GGNCC	5 cut(s)	47, 454, 1164, 1211, 2570
AsuC2I	CCSGG	2 cut(s)	519, 706
AvaI	CYCGRG	1 cut(s)	1530
AvaII	GGWCC	3 cut(s)	454, 1164, 1211
BaeGI	GKGCMC	1 cut(s)	980
BaeI	ACNNNNGTAYC	4 cut(s)	240, 273, 1731, 1764
BalI	TGGCCA	4 cut(s)	761, 1291, 2778, 3047
BamHI	GGATCC	3 cut(s)	261, 1423, 1966
BanI	GGYRCC	1 cut(s)	1067
BanII	GRGCYC	2 cut(s)	513, 2529
BauI	CACGAG	1 cut(s)	203
BbsI	GAAGAC	2 cut(s)	822, 2109
Bbv12I	GWGCWC	4 cut(s)	234, 513, 980, 2825
BbvI	GCAGC	7 cut(s)	1003, 1324, 1421, 2024, 2172, 2389, 2516
BceAI	ACGGC	4 cut(s)	337, 354, 1992, 3140
BciT130I	CCWGG	3 cut(s)	458, 1195, 3232
BclI	TGATCA	2 cut(s)	2152, 2652
BcnI	CCSGG	2 cut(s)	519, 706
BcoDI	GTCTC	2 cut(s)	2164, 3225
BfaI	CTAG	5 cut(s)	1215, 1562, 1679, 1754, 3098
BfmI	CTRYAG	1 cut(s)	1692
BfoI	RGCGCY	3 cut(s)	428, 437, 1597
BfrI	CTTAAG	3 cut(s)	437, 536, 1415
BfuAI	ACCTGC	3 cut(s)	1061, 2299, 2584
BglI	GCCNNNNNGGC	1 cut(s)	422
BglII	AGATCT	1 cut(s)	2420
BmcAI	AGTACT	1 cut(s)	914
Bme1390I	CCNGG	5 cut(s)	458, 519, 706, 1195, 3232
Bme18I	GGWCC	3 cut(s)	454, 1164, 1211
BmeRI	GACNNNNNGTC	1 cut(s)	1858
BmeT110I	CYCGRG	1 cut(s)	1530
BmgT120I	GGNCC	5 cut(s)	47, 454, 1164, 1211, 2570
BmrFI	CCNGG	5 cut(s)	458, 519, 706, 1195, 3232
BmrI	ACTGGG	2 cut(s)	1107, 2821
BmuI	ACTGGG	2 cut(s)	1107, 2821
BpiI	GAAGAC	2 cut(s)	822, 2109
BplI	GAGNNNNNCTC	4 cut(s)	1702, 1734, 2861, 2893
BpmI	CTGGAG	2 cut(s)	449, 642
BpuEI	CTTGAG	4 cut(s)	616, 1631, 1786, 3176
BpuMI	CCSGG	2 cut(s)	519, 706
BsaBI	GATNNNNATC	2 cut(s)	1311, 1878
BsaHI	GRCGYC	2 cut(s)	56, 146
BsaJI	CCNNGG	9 cut(s)	1095, 1390, 1404, 2207, 2340, 2908, 3020, 3032, 3255
BsaWI	WCCGGW	3 cut(s)	661, 752, 2602
Bse118I	RCCGGY	3 cut(s)	499, 655, 3166
Bse1I	ACTGG	7 cut(s)	238, 625, 1011, 1113, 1583, 2272, 2816
Bse3DI	GCAATG	5 cut(s)	418, 1644, 2328, 2328, 3161
Bse8I	GATNNNNATC	2 cut(s)	1311, 1878
BseAI	TCCGGA	2 cut(s)	661, 2602
BseBI	CCWGG	3 cut(s)	458, 1195, 3232
BseDI	CCNNGG	9 cut(s)	1095, 1390, 1404, 2207, 2340, 2908, 3020, 3032, 3255
BseGI	GGATG	9 cut(s)	673, 948, 1252, 1312, 1879, 1907, 2529, 2779, 3001
BseJI	GATNNNNATC	2 cut(s)	1311, 1878
BseMI	GCAATG	5 cut(s)	418, 1644, 2328, 2328, 3161
BseMII	CTCAG	8 cut(s)	1097, 1581, 1733, 1952, 2159, 2779, 2982, 3116
BseNI	ACTGG	7 cut(s)	238, 625, 1011, 1113, 1583, 2272, 2816
BseRI	GAGGAG	4 cut(s)	188, 1994, 2455, 3250
BseSI	GKGCMC	1 cut(s)	980
BseXI	GCAGC	7 cut(s)	1003, 1324, 1421, 2024, 2172, 2389, 2516
BsgI	GTGCAG	1 cut(s)	2031
Bsh1236I	CGCG	4 cut(s)	69, 272, 386, 2660
BshFI	GGCC	9 cut(s)	49, 761, 1291, 2444, 2572, 2778, 3047, 3066, 3155
BshNI	GGYRCC	1 cut(s)	1067
BsiHKAI	GWGCWC	4 cut(s)	234, 513, 980, 2825
BsiHKCI	CYCGRG	1 cut(s)	1530
BslFI	GGGAC	4 cut(s)	721, 1177, 1264, 2829
BsmAI	GTCTC	2 cut(s)	2164, 3225
BsmFI	GGGAC	4 cut(s)	721, 1177, 1264, 2829
BsmI	GAATGC	3 cut(s)	1096, 1401, 2514
BsnI	GGCC	9 cut(s)	49, 761, 1291, 2444, 2572, 2778, 3047, 3066, 3155
BsoBI	CYCGRG	1 cut(s)	1530
Bsp1286I	GDGCHC	5 cut(s)	234, 513, 980, 2529, 2825
Bsp13I	TCCGGA	2 cut(s)	661, 2602
Bsp19I	CCATGG	3 cut(s)	1390, 2908, 3020
Bsp68I	TCGCGA	1 cut(s)	69
BspANI	GGCC	9 cut(s)	49, 761, 1291, 2444, 2572, 2778, 3047, 3066, 3155
BspCNI	CTCAG	8 cut(s)	1096, 1582, 1732, 1953, 2160, 2778, 2983, 3117
BspEI	TCCGGA	2 cut(s)	661, 2602
BspFNI	CGCG	4 cut(s)	69, 272, 386, 2660
BspHI	TCATGA	2 cut(s)	100, 2098
BspMI	ACCTGC	3 cut(s)	1061, 2299, 2584
BspT107I	GGYRCC	1 cut(s)	1067
BspTI	CTTAAG	3 cut(s)	437, 536, 1415
BsrDI	GCAATG	5 cut(s)	418, 1644, 2328, 2328, 3161
BsrFI	RCCGGY	3 cut(s)	499, 655, 3166
BsrI	ACTGG	7 cut(s)	238, 625, 1011, 1113, 1583, 2272, 2816
BssAI	RCCGGY	3 cut(s)	499, 655, 3166
BssECI	CCNNGG	9 cut(s)	1095, 1390, 1404, 2207, 2340, 2908, 3020, 3032, 3255
BssNAI	GTATAC	1 cut(s)	2736
BssNI	GRCGYC	2 cut(s)	56, 146
BssSI	CACGAG	1 cut(s)	203
BssT1I	CCWWGG	7 cut(s)	1095, 1390, 2207, 2908, 3020, 3032, 3255
Bst1107I	GTATAC	1 cut(s)	2736
Bst2BI	CACGAG	1 cut(s)	203
Bst2UI	CCWGG	3 cut(s)	458, 1195, 3232
Bst4CI	ACNGT	6 cut(s)	466, 1141, 1485, 1744, 2713, 2820
Bst6I	CTCTTC	1 cut(s)	2125
BstACI	GRCGYC	2 cut(s)	56, 146
BstAFI	CTTAAG	3 cut(s)	437, 536, 1415
BstC8I	GCNNGC	6 cut(s)	270, 297, 501, 1038, 1289, 1574
BstDEI	CTNAG	8 cut(s)	1083, 1590, 1719, 1961, 2168, 2765, 2991, 3125
BstDSI	CCRYGG	3 cut(s)	1390, 2908, 3020
BstENI	CCTNNNNNAGG	2 cut(s)	2449, 2990
BstF5I	GGATG	9 cut(s)	673, 948, 1252, 1312, 1879, 1907, 2529, 2779, 3001
BstFNI	CGCG	4 cut(s)	69, 272, 386, 2660
BstH2I	RGCGCY	3 cut(s)	428, 437, 1597
BstHHI	GCGC	5 cut(s)	427, 436, 748, 1596, 2660
BstMAI	GTCTC	2 cut(s)	2164, 3225
BstNI	CCWGG	3 cut(s)	458, 1195, 3232
BstSCI	CCNGG	5 cut(s)	456, 517, 704, 1193, 3230
BstSFI	CTRYAG	1 cut(s)	1692
BstSLI	GKGCMC	1 cut(s)	980
BstUI	CGCG	4 cut(s)	69, 272, 386, 2660
BstV1I	GCAGC	7 cut(s)	1003, 1324, 1421, 2024, 2172, 2389, 2516
BstV2I	GAAGAC	2 cut(s)	822, 2109
BstX2I	RGATCY	5 cut(s)	261, 1423, 1966, 2420, 3186
BstYI	RGATCY	5 cut(s)	261, 1423, 1966, 2420, 3186
BstZ17I	GTATAC	1 cut(s)	2736
BsuRI	GGCC	9 cut(s)	49, 761, 1291, 2444, 2572, 2778, 3047, 3066, 3155
BtgI	CCRYGG	3 cut(s)	1390, 2908, 3020
BtgZI	GCGATG	1 cut(s)	84
BtsCI	GGATG	9 cut(s)	673, 948, 1252, 1312, 1879, 1907, 2529, 2779, 3001
BtsI	GCAGTG	1 cut(s)	1005
BtsIMutI	CAGTG	9 cut(s)	1004, 1005, 1120, 1481, 1576, 2562, 2648, 2773, 2825
BtuMI	TCGCGA	1 cut(s)	69
BveI	ACCTGC	3 cut(s)	1061, 2299, 2584
Cac8I	GCNNGC	6 cut(s)	270, 297, 501, 1038, 1289, 1574
CciI	TCATGA	2 cut(s)	100, 2098
CfoI	GCGC	5 cut(s)	427, 436, 748, 1596, 2660
Cfr10I	RCCGGY	3 cut(s)	499, 655, 3166
Cfr13I	GGNCC	5 cut(s)	47, 454, 1164, 1211, 2570
Csp6I	GTAC	9 cut(s)	178, 913, 1068, 1134, 1748, 2094, 2112, 2455, 3119
CspCI	CAANNNNNGTGG	2 cut(s)	751, 786
CviQI	GTAC	9 cut(s)	178, 913, 1068, 1134, 1748, 2094, 2112, 2455, 3119
DdeI	CTNAG	8 cut(s)	1083, 1590, 1719, 1961, 2168, 2765, 2991, 3125
DraIII	CACNNNGTG	1 cut(s)	809
DrdI	GACNNNNNNGTC	1 cut(s)	3217
DriI	GACNNNNNGTC	1 cut(s)	1858
DseDI	GACNNNNNNGTC	1 cut(s)	3217
EaeI	YGGCCR	5 cut(s)	759, 1289, 2776, 3045, 3153
Eam1104I	CTCTTC	1 cut(s)	2125
Eam1105I	GACNNNNNGTC	1 cut(s)	1858
EarI	CTCTTC	1 cut(s)	2125
EciI	GGCGGA	1 cut(s)	167
Ecl136II	GAGCTC	1 cut(s)	511
Eco130I	CCWWGG	7 cut(s)	1095, 1390, 2207, 2908, 3020, 3032, 3255
Eco147I	AGGCCT	1 cut(s)	2444
Eco24I	GRGCYC	2 cut(s)	513, 2529
Eco47I	GGWCC	3 cut(s)	454, 1164, 1211
Eco53kI	GAGCTC	1 cut(s)	511
Eco57I	CTGAAG	3 cut(s)	1047, 1542, 2541
Eco88I	CYCGRG	1 cut(s)	1530
EcoICRI	GAGCTC	1 cut(s)	511
EcoNI	CCTNNNNNAGG	2 cut(s)	2449, 2990
EcoO109I	RGGNCCY	2 cut(s)	47, 1164
EcoRI	GAATTC	1 cut(s)	2019
EcoRII	CCWGG	3 cut(s)	456, 1193, 3230
EcoT14I	CCWWGG	7 cut(s)	1095, 1390, 2207, 2908, 3020, 3032, 3255
EcoT38I	GRGCYC	2 cut(s)	513, 2529
ErhI	CCWWGG	7 cut(s)	1095, 1390, 2207, 2908, 3020, 3032, 3255
FalI	AAGNNNNNCTT	2 cut(s)	1079, 1111
FaqI	GGGAC	4 cut(s)	721, 1177, 1264, 2829
FauI	CCCGC	4 cut(s)	37, 162, 1079, 2008
FbaI	TGATCA	2 cut(s)	2152, 2652
FblI	GTMKAC	1 cut(s)	2735
FokI	GGATG	9 cut(s)	680, 955, 1259, 1319, 1866, 1894, 2516, 2786, 3008
FriOI	GRGCYC	2 cut(s)	513, 2529
FspBI	CTAG	5 cut(s)	1215, 1562, 1679, 1754, 3098
FspI	TGCGCA	1 cut(s)	747
GlaI	GCGC	5 cut(s)	426, 435, 747, 1595, 2659
GsuI	CTGGAG	2 cut(s)	449, 642
HaeII	RGCGCY	3 cut(s)	428, 437, 1597
HaeIII	GGCC	9 cut(s)	49, 761, 1291, 2444, 2572, 2778, 3047, 3066, 3155
HhaI	GCGC	5 cut(s)	427, 436, 748, 1596, 2660
Hin1I	GRCGYC	2 cut(s)	56, 146
Hin6I	GCGC	5 cut(s)	425, 434, 746, 1594, 2658
HinP1I	GCGC	5 cut(s)	425, 434, 746, 1594, 2658
HincII	GTYRAC	3 cut(s)	618, 1471, 3028
HindII	GTYRAC	3 cut(s)	618, 1471, 3028
HindIII	AAGCTT	3 cut(s)	836, 1106, 2355
HpaI	GTTAAC	1 cut(s)	1471
Hpy166II	GTNNAC	7 cut(s)	382, 618, 978, 1298, 1471, 2736, 3028
Hpy8I	GTNNAC	7 cut(s)	382, 618, 978, 1298, 1471, 2736, 3028
Hpy99I	CGWCG	4 cut(s)	58, 61, 64, 151
HpyCH4III	ACNGT	6 cut(s)	466, 1141, 1485, 1744, 2713, 2820
HpyCH4IV	ACGT	4 cut(s)	56, 146, 1300, 1816
HpyF3I	CTNAG	8 cut(s)	1083, 1590, 1719, 1961, 2168, 2765, 2991, 3125
HpySE526I	ACGT	4 cut(s)	56, 146, 1300, 1816
Hsp92I	GRCGYC	2 cut(s)	56, 146
HspAI	GCGC	5 cut(s)	425, 434, 746, 1594, 2658
Kpn2I	TCCGGA	2 cut(s)	661, 2602
KpnI	GGTACC	1 cut(s)	1071
KroI	GCCGGC	1 cut(s)	499
KroNI	GCCGGC	1 cut(s)	501
Ksp22I	TGATCA	2 cut(s)	2152, 2652
KspAI	GTTAAC	1 cut(s)	1471
LmnI	GCTCC	4 cut(s)	349, 508, 532, 3042
Lsp1109I	GCAGC	7 cut(s)	1003, 1324, 1421, 2024, 2172, 2389, 2516
MaeI	CTAG	5 cut(s)	1215, 1562, 1679, 1754, 3098
MaeII	ACGT	4 cut(s)	56, 146, 1300, 1816
MaeIII	GTNAC	3 cut(s)	950, 2560, 2788
MfeI	CAATTG	2 cut(s)	1995, 2394
MflI	RGATCY	5 cut(s)	261, 1423, 1966, 2420, 3186
MhlI	GDGCHC	5 cut(s)	234, 513, 980, 2529, 2825
MlsI	TGGCCA	4 cut(s)	761, 1291, 2778, 3047
MluNI	TGGCCA	4 cut(s)	761, 1291, 2778, 3047
MlyI	GAGTC	3 cut(s)	232, 1868, 3136
MmeI	TCCRAC	9 cut(s)	42, 174, 285, 373, 432, 1686, 1936, 2385, 2680
Mox20I	TGGCCA	4 cut(s)	761, 1291, 2778, 3047
MroI	TCCGGA	2 cut(s)	661, 2602
MroNI	GCCGGC	1 cut(s)	499
MroXI	GAANNNNTTC	1 cut(s)	116
MscI	TGGCCA	4 cut(s)	761, 1291, 2778, 3047
MseI	TTAA	9 cut(s)	438, 537, 840, 1239, 1416, 1470, 1791, 2595, 2922
MslI	CAYNNNNRTG	4 cut(s)	120, 892, 1581, 2963
Msp20I	TGGCCA	4 cut(s)	761, 1291, 2778, 3047
MspA1I	CMGCKG	2 cut(s)	2015, 2861
MspCI	CTTAAG	3 cut(s)	437, 536, 1415
MspR9I	CCNGG	5 cut(s)	458, 519, 706, 1195, 3232
MunI	CAATTG	2 cut(s)	1995, 2394
Mva1269I	GAATGC	3 cut(s)	1096, 1401, 2514
MvaI	CCWGG	3 cut(s)	458, 1195, 3232
MvnI	CGCG	4 cut(s)	69, 272, 386, 2660
NaeI	GCCGGC	1 cut(s)	501
NciI	CCSGG	2 cut(s)	519, 706
NcoI	CCATGG	3 cut(s)	1390, 2908, 3020
NgoMIV	GCCGGC	1 cut(s)	499
NmeAIII	GCCGAG	3 cut(s)	442, 972, 1622
NmuCI	GTSAC	1 cut(s)	2560
NruI	TCGCGA	1 cut(s)	69
NsbI	TGCGCA	1 cut(s)	747
OliI	CACNNNNGTG	1 cut(s)	120
PaeR7I	CTCGAG	1 cut(s)	1530
PagI	TCATGA	2 cut(s)	100, 2098
PaqCI	CACCTGC	2 cut(s)	2299, 2584
PceI	AGGCCT	1 cut(s)	2444
PcsI	WCGNNNNNNNCGW	2 cut(s)	186, 458
PctI	GAATGC	3 cut(s)	1096, 1401, 2514
PdiI	GCCGGC	1 cut(s)	501
PdmI	GAANNNNTTC	1 cut(s)	116
PfeI	GAWTC	8 cut(s)	97, 370, 445, 469, 1586, 2143, 2645, 3172
PflFI	GACNNNGTC	1 cut(s)	58
PflMI	CCANNNNNTGG	1 cut(s)	1271
PfoI	TCCNGGA	2 cut(s)	517, 3230
PleI	GAGTC	3 cut(s)	231, 1867, 3135
PpsI	GAGTC	3 cut(s)	231, 1867, 3135
PpuMI	RGGWCCY	1 cut(s)	1164
Psp124BI	GAGCTC	1 cut(s)	513
Psp5II	RGGWCCY	1 cut(s)	1164
Psp6I	CCWGG	3 cut(s)	456, 1193, 3230
PspGI	CCWGG	3 cut(s)	456, 1193, 3230
PspPI	GGNCC	5 cut(s)	47, 454, 1164, 1211, 2570
PspPPI	RGGWCCY	1 cut(s)	1164
PsuI	RGATCY	5 cut(s)	261, 1423, 1966, 2420, 3186
PsyI	GACNNNGTC	1 cut(s)	58
PvuII	CAGCTG	1 cut(s)	2861
RruI	TCGCGA	1 cut(s)	69
RsaI	GTAC	9 cut(s)	179, 914, 1069, 1135, 1749, 2095, 2113, 2456, 3120
RsaNI	GTAC	9 cut(s)	178, 913, 1068, 1134, 1748, 2094, 2112, 2455, 3119
RseI	CAYNNNNRTG	4 cut(s)	120, 892, 1581, 2963
SacI	GAGCTC	1 cut(s)	513
SaqAI	TTAA	9 cut(s)	438, 537, 840, 1239, 1416, 1470, 1791, 2595, 2922
Sau96I	GGNCC	5 cut(s)	47, 454, 1164, 1211, 2570
ScaI	AGTACT	1 cut(s)	914
SchI	GAGTC	3 cut(s)	232, 1868, 3136
ScrFI	CCNGG	5 cut(s)	458, 519, 706, 1195, 3232
SduI	GDGCHC	5 cut(s)	234, 513, 980, 2529, 2825
SfcI	CTRYAG	1 cut(s)	1692
Sfr274I	CTCGAG	1 cut(s)	1530
SinI	GGWCC	3 cut(s)	454, 1164, 1211
SlaI	CTCGAG	1 cut(s)	1530
SmiMI	CAYNNNNRTG	4 cut(s)	120, 892, 1581, 2963
SmlI	CTYRAG	8 cut(s)	437, 536, 595, 1415, 1530, 1610, 1765, 3191
SmoI	CTYRAG	8 cut(s)	437, 536, 595, 1415, 1530, 1610, 1765, 3191
SseBI	AGGCCT	1 cut(s)	2444
SspI	AATATT	2 cut(s)	2473, 3108
SspMI	CTAG	5 cut(s)	1215, 1562, 1679, 1754, 3098
SstI	GAGCTC	1 cut(s)	513
StuI	AGGCCT	1 cut(s)	2444
StyD4I	CCNGG	5 cut(s)	456, 517, 704, 1193, 3230
StyI	CCWWGG	7 cut(s)	1095, 1390, 2207, 2908, 3020, 3032, 3255
TaaI	ACNGT	6 cut(s)	466, 1141, 1485, 1744, 2713, 2820
TaiI	ACGT	4 cut(s)	59, 149, 1303, 1819
TaqI	TCGA	8 cut(s)	215, 234, 590, 990, 1531, 2760, 2961, 3248
TaqII	GACCGA	1 cut(s)	1199
TatI	WGTACW	2 cut(s)	912, 2111
TauI	GCSGC	5 cut(s)	44, 169, 275, 278, 2916
TfiI	GAWTC	8 cut(s)	97, 370, 445, 469, 1586, 2143, 2645, 3172
Tru1I	TTAA	9 cut(s)	438, 537, 840, 1239, 1416, 1470, 1791, 2595, 2922
Tru9I	TTAA	9 cut(s)	438, 537, 840, 1239, 1416, 1470, 1791, 2595, 2922
TscAI	CASTG	9 cut(s)	1005, 1011, 1120, 1488, 1583, 2569, 2655, 2773, 2825
TseFI	GTSAC	1 cut(s)	2560
TseI	GCWGC	7 cut(s)	1016, 1337, 1409, 2012, 2185, 2402, 2504
Tsp45I	GTSAC	1 cut(s)	2560
TspGWI	ACGGA	3 cut(s)	813, 1246, 1416
TspRI	CASTG	9 cut(s)	1005, 1011, 1120, 1488, 1583, 2569, 2655, 2773, 2825
Tth111I	GACNNNGTC	1 cut(s)	58
Van91I	CCANNNNNTGG	1 cut(s)	1271
Vha464I	CTTAAG	3 cut(s)	437, 536, 1415
VneI	GTGCAC	1 cut(s)	976
VpaK11BI	GGWCC	3 cut(s)	454, 1164, 1211
XagI	CCTNNNNNAGG	2 cut(s)	2449, 2990
XapI	RAATTY	5 cut(s)	493, 789, 1278, 1669, 2019
XhoI	CTCGAG	1 cut(s)	1530
XmiI	GTMKAC	1 cut(s)	2735
XmnI	GAANNNNTTC	1 cut(s)	116
XspI	CTAG	5 cut(s)	1215, 1562, 1679, 1754, 3098
ZraI	GACGTC	2 cut(s)	57, 147
ZrmI	AGTACT	1 cut(s)	914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.